Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F1 I0 R1
|
4577 |
57.4 |
3436498 |
77.7% |
2670158 |
63.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
464,269 |
A→G |
40.9% |
L42L (CTA→CTG) |
sdhB → |
succinate dehydrogenase, FeS subunit |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 464,269 | 0 | A | G | 40.9%
| 22.2
/ 47.9
| 66 | L42L (CTA→CTG) | sdhB | succinate dehydrogenase, FeS subunit |
| Reads supporting (aligned to +/- strand): ref base A (17/22); new base G (10/17); total (27/39) |
| Fisher's exact test for biased strand distribution p-value = 6.21e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.11e-01 |
CAGGATTACACCCTGGAAGCGGATGAAGGTCGCGACATGATGCTGCTGGATGCGCTTATCCAGCTAAAAGAGAAAGATCCCAGCCTGTCGTTCCGCCGCTCCTGCCGTGAAGGTGTGTGCGGTTCCGACGG > minE/464204‑464334
|
cAGGATTACACCCTGGAAGCGGATGAAGGTCGCGACATGATGCTGCTGGATGCGCTTATCCAGCTaa > 1:974140/1‑67 (MQ=255)
cAGGATTACACCCTGGAAGCGGATGAAGGTCGCGACATGATGCTGCTGGATGCGCTTATCCAGCTaa > 1:96890/1‑67 (MQ=255)
cAGGATTACACCCTGGAAGCGGATGAAGGTCGCGACATGATGCTGCTGGATGCGCTTATCCAGCTaa > 1:610379/1‑67 (MQ=255)
cAGGATTACACCCTGGAAGCGGATGAAGGTCGCGACATGATGCTGCTGGATGCGCTTATCCAGCTaa > 1:431558/1‑67 (MQ=255)
cAGGATTACACCCTGGAAGCGGATGAAGGTCGCGACATGATGCTGCTGGATGCGCTTATCCAGCTaa > 1:398070/1‑67 (MQ=255)
cAGGATTACACCCTGGAAGCGGATGAAGGTCGCGACATGATGCTGCTGGATGCGCTTATCCAGCTaa > 1:3377841/1‑67 (MQ=255)
cAGGATTACACCCTGGAAGCGGATGAAGGTCGCGACATGATGCTGCTGGATGCGCTTATCCAGCTaa > 1:2234041/1‑67 (MQ=255)
cAGGATTACACCCTGGAAGCGGATGAAGGTCGCGACATGATGCTGCTGGATGCGCTTATCCAGCTaa > 1:1480450/1‑67 (MQ=255)
cAGGATTACACCCTGGAAGCGGATGAAGGTCGCGACATGATGCTGCTGGATGCGCTTATCCAGCTa > 1:1055595/1‑66 (MQ=255)
cAGGATTACACCCTGGAAGCGGATGAAGGTCGCGACATGATGCTGCTGGATGCGCTTATCCAGATaa > 1:2544083/1‑67 (MQ=255)
cAGGATTACACCCTAGAAGCGGATGAAGGTCGCGACATGATGCTGCTGGATGCGCTTATCCAGCTaa > 1:1262063/1‑67 (MQ=255)
ttACACCCTGGAAGCGGATGAAGGTCGCGACATGATGCTGCTGGATGCGCTTATCCAGCTAAAaga > 1:288879/1‑66 (MQ=255)
ttACACCCTGGAAGCGGATGAAGGTCGCGACATGATGCTGCTGGATGCGCTTATCCAGCTAAAaga > 1:2362094/1‑66 (MQ=255)
ttACACCCTGGAAGCGGATGAAGGTCGCGACATGATGCTGCTGGATGCGCTTATCCAGCTAAAaga > 1:2349463/1‑66 (MQ=255)
ttACACCCTGGAAGCGGATGAAGGTCGCGACATGATGCTGCTGGATGCGCTTATCCAGCTAAAaga > 1:3271268/1‑66 (MQ=255)
ttACACCCTGGAAGCGGATGAAGGTCGCGACATGATGCTGCTGGATGCGCTTATCCAGCTAAAaga > 1:330986/1‑66 (MQ=255)
ttACACCCTGGAAGCGGATGAAGGTCGCGACATGATGCTGCTGGATGCGCTTATCCAGCTAAAaga > 1:1650151/1‑66 (MQ=255)
ttACACCCTGGAAGCGGATGAAGGTCGCGACATGATGCTGCTGGATGCGCTTATCCAGCTAAAaga > 1:1718589/1‑66 (MQ=255)
acacCCTGGAAGCGGATGAAGGTCGCGACATGATGCTGCTGGATGCGCTTATCCAGCTaa > 1:2594964/1‑60 (MQ=255)
acacCCTGGAAGCGGATGAAGGTCGCGACATGATGCTGCTGGATGCGCTTATCCAGCTaa > 1:1830372/1‑60 (MQ=255)
acacCCTGGAAGCGGATGAAGGTCGCGACATGATGCTGCTGGATGCGCTTATCCAGCTaa > 1:1971804/1‑60 (MQ=255)
acacCCTGGAAGCGGATGAAGGTCGCGACATGATGCTGCTGGATGCGCTTATCCAGCTaa > 1:1746848/1‑60 (MQ=255)
acacCCTGGAAGCGGATGAAGGTCGCGACATGATGCTGCTGGATGCGCTTATCCAGCTaa > 1:398401/1‑60 (MQ=255)
cccTGGAAGCGGAAGAAGGTCGCGACATGATGCTGCTGGATGCGCTTATTCAGCTGAAAGAGGAAGAt > 1:3375203/1‑68 (MQ=255)
cccTGGAAGCGGAAGAAGGTCGCGACATGATGCTGCTGGATGCGCTTATTCAGCTGAAAGAGAAAGAt > 1:2165846/1‑68 (MQ=255)
cccTGGAAGCGGAAGAAGGTCGCGACATGATGCTGCTGGATGCGCTTATTCAGCTGAAAGAGAAAGAt > 1:840843/1‑68 (MQ=255)
cccTGGAAGCGGAAGAAGGTCGCGACATGATGCTGCTGGATGCGCTTATTCAGCTGAAAGAGAAAGAt > 1:2192077/1‑68 (MQ=255)
cccTGGAAGCGGAAGAAGGTCGCGACATGATGCTGCTGGATGCGCTTATTCAGCTGAAAGAGAAAGAt > 1:872885/1‑68 (MQ=255)
cccTGGAAGCGGAAGAAGGTCGCGACATGATGCTGCTGGATGCGCTTATTCAGCTGAAAGAGAAAGAt > 1:3191684/1‑68 (MQ=255)
cccTGGAAGCGGAAGAAGGTCGCGACATGATGCTGCTGGATGCGCTTATTCAGCTGAAAGAGAAAGAt > 1:959612/1‑68 (MQ=255)
cccTGGAAGCGGAAGAAGGTCGCGACATGATGCTGCTGGATGCGCTTATTCAGCTGAAAGAGAAAGAt > 1:315961/1‑68 (MQ=255)
cccTGGAAGCGGAAGAAGGTCGCGACATGATGCTGCTGGATGCGCTTATTCAGATGAAAGAGAAAGAt > 1:1921481/1‑68 (MQ=255)
cccTGCAAGCGGAAGAAGGTCGCGACATGATGCTGCTGGATGCGCTTATTCAGCTGAAAGAGAAAGAt > 1:2239057/1‑68 (MQ=255)
aGCGGAAGATGGTCGCGACATGATGCTGCTGGATGCGCTTATTCAGCTGAAAGTGAAAGATCCCAGc < 1:3390465/67‑1 (MQ=255)
aGCGGAAGAAGGTCGCGACATGATGCTGCTGGATGCGCTTATTCAGCTGAAAGAGAAAGATCCCAGc < 1:2874834/67‑1 (MQ=255)
aGCGGAAGAAGGTCGCGACATGATGCTGCTGGATGCGCTTATTCAGCTGAAAGAGAAAGATCCCAGc < 1:1037290/67‑1 (MQ=255)
aGCGGAAGAAGGTCGCGACATGATGCTGCTGGATGCGCTTATTCAGCTGAAAGAGAAAGATCCCAGc < 1:2825482/67‑1 (MQ=255)
aGCGGAAGAAGGTCGCGACATGATGCTGCTGGATGCGCTTATTCAGCTGAAAGAGAAAGATCCCAGc < 1:1157106/67‑1 (MQ=255)
aGCGGAAGAAGGTCGCGACATGATGCTGCTGGATGCGCTTATTCAGCTGAAAGAGAAAGATCCCAGc < 1:769529/67‑1 (MQ=255)
aGCGGAAGAAGGTCGCGACATGATGCTGCTGGATGCGCTTATTCAGCTGAAAGAGAAAGATCCCAGc < 1:81480/67‑1 (MQ=255)
aGCGGAAGAAGGTCGCGACATGATGCTGCTGGATGCGCTTATTCAGCTGAAAGAGAAAGATCCCAGc < 1:1097082/67‑1 (MQ=255)
aGCGGAAGAAGGTCGCGACATGATGCTGCTGGATGCGCTTATTCAGCTGAAAGAGAAAGATCCCAGc < 1:2799478/67‑1 (MQ=255)
aGCGGAAGAAGGTCGCGACATGATGCTGCTGGATGCGCTTATTCAGCTGAAAGAGAAAGATCCCAGc < 1:1493266/67‑1 (MQ=255)
aGCGGAAGAAGGTCGCGACATGATGCTGCTGGATGCGCTTATTCAGCTGAAAGAGAAAGATCCCAGc < 1:1570583/67‑1 (MQ=255)
aGCGGAAGAAGGTCGCGACATGATGCTGCTGGATGCGCTTATTCAGCTGAAAGAGAAAGATCCCAGc < 1:2154083/67‑1 (MQ=255)
aGCGGAAGAAGGTCGCGACATGATGCTGCTGGATGCGCTTATTCAGCTGAAAGAGAAAGATCCCAGc < 1:2099582/67‑1 (MQ=255)
aGCGGAAGAAGGTCGCGACATGATGCTGCTGGATGCGCTTATTCAGCTGAAAGAGAAAGATCCCAGc < 1:1756769/67‑1 (MQ=255)
aGCGGAAGAAGGTCGCGACATGATGCTGCTGGATGCGCTTATTCAGCTGAAAGAGAAAGATCCCAGc < 1:2226435/67‑1 (MQ=255)
gCGGAAGAAGGTCGCGACATGATGCTGCTGGATGCGCTTATTCAGCTGAAAGAGAAAGATCCCAGc < 1:342497/66‑1 (MQ=255)
cGGAAGAAGGTCGCGACATGATGCTGCTGGATGCGCTTATTCAGCTGAAAGAGAAAGATCCCAGc < 1:839980/65‑1 (MQ=255)
aGGTCGCGACATGATGCTGCTGGATGCGCTTATCCAGCTAAAAGAGAAAGATCCCAGCCTGTCGTTccg > 1:709383/1‑69 (MQ=255)
aGGTCGCGACATGATGCTGCTGGATGCGCTTATCCAGCTAAAAGAGAAAGATCCCAGCCTGTCGTTccg > 1:2436739/1‑69 (MQ=255)
aGGTCGCGACATGATGCTGCTGGATGCGCTTATCCAGCTAAAAGAGAAAGATCCCAGCCTGTCGTTccg > 1:49810/1‑69 (MQ=255)
aGGTCGCGACATGATGCTGCTGGATGCGCTTATCCAGCTAAAAGAGAAAGATCCCAGCCTGTCGTTccg > 1:3369704/1‑69 (MQ=255)
aGGTCGCGACATGATGCTGCTGGATGCGCTTATCCAGCTAAAAGAGAAAGATCCCAGCCTGTCGTTccg > 1:2107991/1‑69 (MQ=255)
atgatgCTGCTGGATGCGCTTATCCAGCTAAAAGAGAAAGATCCCAGCCTGTCGTTCCGCCGCTCCTGc < 1:60508/69‑1 (MQ=255)
atgatgCTGCTGGATGCGCTTATCCAGCTAAAAGAGAAAGATCCCAGCCTGTCGTTCCGCCGCTCCTGc < 1:791994/69‑1 (MQ=255)
atgatgCTGCTGGATGCGCTTATCCAGCTAAAAGAGAAAGATCCCAGCCTGTCGTTCCGCCGCTCCTGc < 1:558553/69‑1 (MQ=255)
atgatgCTGCTGGATGCGCTTATCCAGCTAAAAGAGAAAGATCCCAGCCTGTCGTTCCGCCGCTCCTGc < 1:1145782/69‑1 (MQ=255)
atgatgCTGCTGGATGCGCTTATCCAGCTAAAAGAGAAAGATCCCAGCCTGTCGTTCCGCCGCTCCTGc < 1:946886/69‑1 (MQ=255)
atgatgCTGCTGGATGCGCTTATCCAGCTAAAAGAGAAAGATCCCAGCCTGTCGTTCCGCCGCTCCTGc < 1:1458258/69‑1 (MQ=255)
atgatgCTGCTGGATGCGCTTATCCAGCTAAAAGAGAAAGATCCCAGCCTGTCGTTCCGCCGCTCCTGc < 1:3319099/69‑1 (MQ=255)
atgatgCTGCTGGATGCGCTTATCCAGCTAAAAGAGAAAGATCCCAGCCTGTCGTTCCGCCGCTCCTGc < 1:3003922/69‑1 (MQ=255)
atgatgCTGCTGGATGCGCTTATCCAGCTAAAAGAGAAAGATCCCAGCCTGTCGTTCCGCCGCTCCTGc < 1:2347163/69‑1 (MQ=255)
tgatgCTGCTGGATGCGCTTATCCAGCTAAAAGAGAAAGATCCCAGCCTGTCGTTCCGCCGCTCCTGc < 1:2516886/68‑1 (MQ=255)
atgCTGCTGGATGCGCTTATCCAGCTAAAAGAGAAAGATCCCAGCCTGTCGTTCCGCCGCTCCTGCCg > 1:1161396/1‑68 (MQ=255)
atgCTGCTGGATGCGCTTATCCAGCTAAAAGAGAAAGATCCCAGCCTGTCGTTCCGCCGCTCCTGCCg > 1:2080452/1‑68 (MQ=255)
gctgctGGATGCGCTTATCCAGCTAAAAGAGAAAGATCCCAGCCTGTCGTTCCGCCGCTCCTGCCGTg < 1:3383054/68‑1 (MQ=255)
gctgctGGATGCGCTTATCCAGCTAAAAGAGAAAGATCCCAGCCTGTCGTTCCGCCGCTCCTGCCGTGa > 1:1107433/1‑69 (MQ=255)
gctgctGGATGCGCTTATCCAGCTAAAAGAGAAAGATCCCAGCCTGTCGTTCCGCCGCTCCTGCCGTGa > 1:1625766/1‑69 (MQ=255)
gctgctGGATGCGCTTATCCAGCTAAAAGAGAAAGATCCCAGCCTGTCGTTCCGCCGCTCCTGCCGTGa > 1:3041074/1‑69 (MQ=255)
gcgcTTATCCAGCTAAAAGAGAAAGATCCCAGCCTGTCGTTCCGCCGCTCCTGCCGTg < 1:578626/58‑1 (MQ=255)
gcgcTTATCCAGCTAAAAGAGAAAGATCCCAGCCTGTCGTTCCGCCGCTCCTGCCGTg < 1:2189278/58‑1 (MQ=255)
cTTATCCAGCTAAAAGAGAAAGATCCCAGCCTGTCGTTCCGCCGCTCCAGCCGTg < 1:3127780/55‑1 (MQ=255)
gCTAAAAGAGAAAGATCCCAGCCTGTCTTTCCGCCGCTCCTGCCGTTAAGGTGTGTGCGGTTCCTACgg < 1:2895926/69‑1 (MQ=255)
gCTAAAAGAGAAAGATCCCAGCCTGTCGTTCCGCCGCTCCTGCCGTGAAGGTGTGTGCGGTTCCGACgg < 1:2611890/69‑1 (MQ=255)
gCTAAAAGAGAAAGATCCCAGCCTGTCGTTCCGCCGCTCCTGCCGTGAAGGTGTGTGCGGTTCCGACgg < 1:2882030/69‑1 (MQ=255)
gCTAAAAGAGAAAGATCCCAGCCTGTCGTTCCGCCGCTCCTGCCGTGAAGGTGTGTGCGGTTCCGACgg < 1:2568207/69‑1 (MQ=255)
gCTAAAAGAGAAAGATCCCAGCCTGTCGTTCCGCCGCTCCTGCCGTGAAGGTGTGTGCGGTTCCGACgg < 1:3417463/69‑1 (MQ=255)
gCTAAAAGAGAAAGATCCCAGCCTGTCGTTCCGCCGCTCCTGCCGTGAAGGTGTGTGCGGTTCCGACgg < 1:16741/69‑1 (MQ=255)
gCTAAAAGAGAAAGATCCCAGCCTGTCGTTCCGCCGCTCCTGCCGTGAAGGTGTGTGCGGTTCCGACgg < 1:2166574/69‑1 (MQ=255)
gCTAAAAGAGAAAGATCCCAGCCTGTCGTTCCGCCGCTCCTGCCGTGAAGGTGTGTGCGGTTCCGACgg < 1:351788/69‑1 (MQ=255)
|
CAGGATTACACCCTGGAAGCGGATGAAGGTCGCGACATGATGCTGCTGGATGCGCTTATCCAGCTAAAAGAGAAAGATCCCAGCCTGTCGTTCCGCCGCTCCTGCCGTGAAGGTGTGTGCGGTTCCGACGG > minE/464204‑464334
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
