Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F1 I0 R1
|
4577 |
57.4 |
3436498 |
77.7% |
2670158 |
63.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
739,219 |
C→T |
29.5% |
A530T (GCT→ACT) |
fhuE ← |
ferric‑rhodotorulic acid outer membrane transporter |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 739,219 | 0 | C | T | 29.5%
| 92.2
/ 39.2
| 92 | A530T (GCT→ACT) | fhuE | ferric‑rhodotorulic acid outer membrane transporter |
| Reads supporting (aligned to +/- strand): ref base C (20/44); new base T (6/21); total (26/66) |
| Fisher's exact test for biased strand distribution p-value = 4.54e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
CAGACGGCTATTCATCCAGTCCGATTTCAGACCCAGCTCGTAGTTGTTACCGGTGATTGGAGCCAGATATTTGCCTGAACTGTCACGATCATTTTGCGGCTGGAAAATAGAGGTATAGCTGGC > minE/739157‑739279
|
cAGACGGCTATTCATCCAGTCCGATTTCAGACGCAGCTCGTAGTTGTTACCGGTGATTGGAGCCAGat < 1:2237713/68‑1 (MQ=255)
cAGACGGCTATTCATCCAGTCCGATTTCAGACCCAGCTCGTAGTTGTTACGGGTGATTGGAGCCAGat < 1:3228232/68‑1 (MQ=255)
cAGACGGCTATTCATCCAGTCCGATTTCAGACCCAGCTCGTAGTTGTTACCGGTGATTGGAGCCAGata < 1:2796813/69‑1 (MQ=255)
cAGACGGCTATTCATCCAGTCCGATTTCAGACCCAGCTCGTAGTTGTTACCGGTGATTGGAGCCAGata < 1:900883/69‑1 (MQ=255)
cAGACGGCTATTCATCCAGTCCGATTTCAGACCCAGCTCGTAGTTGTTACCGGTGATTGGAGCCAGata < 1:3246615/69‑1 (MQ=255)
cAGACGGCTATTCATCCAGTCCGATTTCAGACCCAGCTCGTAGTTGTTACCGGTGATTGGAGCCAGat < 1:2812247/68‑1 (MQ=255)
cAGACGGCTATTCATCCAGTCCGATTTCAGACCCAGCTCGTAGTTGTTACCGGTGATTGGAGCCAGat < 1:3015586/68‑1 (MQ=255)
cAGACGGCTATTCATCCAGTCCGATTTCAGACCCAGCTCGTAGTTGTTACCGGTGATTGGAGCCAGat < 1:3179915/68‑1 (MQ=255)
aGACGGCTATTCATCCAGTCCGATTTCAGACCCAGCTCTTAGTTGTTACCGGTGATTGTAGCCAGat < 1:302404/67‑1 (MQ=255)
aTTCATCCAGTCCGATTTCAGACCCAGGTCGTAGTTGTTACCGGTGATTGGAGTCAGATATTTGCCTGa > 1:842695/1‑69 (MQ=255)
aTTCATCCAGTCCGATTTCAGACCCAGCTCGTAGTTGTTACCGGTGATTGGAGTCAGATATTTGCCTg > 1:936668/1‑68 (MQ=255)
aTTCATCCAGTCCGATTTCAGACCCAGCTCGTAGTTGTTACCGGTGATTGGAGTCAGATATTTGCCTGa > 1:2244985/1‑69 (MQ=255)
aTTCATCCAGTCCGATTTCAGACCCAGCTCGTAGTTGTTACCGGTGATTGGAGTCAGATATTTGCCTGa > 1:847883/1‑69 (MQ=255)
aTTCATCCAGTCCGATTTCAGACCCAGCTCGTAGTTGTTACCGGTGATTGGAGTCAGATATTTGCCTGa > 1:3007428/1‑69 (MQ=255)
aTTCATCCAGTCCGATTTCAGACCCAGCTCGTAGTTGTTACCGGTGATTGGAGTCAGATATTTGCCTGa > 1:1651534/1‑69 (MQ=255)
cAGTCCGATTTCAGACCCAGCTCGTAGTTGTTACCGGTGATTGGAGCCAGATATTTGCCTGAACTGTc > 1:3335276/1‑68 (MQ=255)
cAGTCCGATTTCAGACCCAGCTCGTAGTTGTTACCGGTGATTGGAGCCAGATATTTGCCTGAACTGTc > 1:979564/1‑68 (MQ=255)
cAGTCCGATTTCAGACCCAGCTCGTAGTTGTTACCGGTGATTGGAGCCAGATATTTGCCTGAACTGTc > 1:1853433/1‑68 (MQ=255)
cAGTCCGATTTCAGACCCAGCTCGTAGTTGTTACCGGTGATTGGAGCCAGATATTTGCCTGAACTGTc > 1:1714325/1‑68 (MQ=255)
cAGTCCGATTTCAGACCCAGCTCGTAGTTGTTACCGGTGATTGGAGCCAGATATTTGCCTGAACTGTc > 1:1487318/1‑68 (MQ=255)
cAGTCCGATTTCAGACCCAGCTCGTAGTTGTTACCGGTGATTGGAGCCAGATATTTGCCTGAACTGTc > 1:3362385/1‑68 (MQ=255)
cAGTCCGATTTCAGACCCAGCTCGTAGTTGTTACCGGTGATTGGAGCCAGATATTTGCCTGAACTGTc > 1:1328277/1‑68 (MQ=255)
cccAGCTCGTAGTTGTTACCGGTGATTGGAGCCAGATATTTGCCTGAACTGTCACGATCATTTTGCGGc < 1:2898520/69‑1 (MQ=255)
cccAGCTCGTAGTTGTTACCGGTGATTGGAGCCAGATATTTGCCTGAACTGTCACGATCATTTTGCGGc < 1:2905731/69‑1 (MQ=255)
cccAGCTCGTAGTTGTTACCGGTGATTGGAGCCAGATATTTGCCTGAACTGTCACGATCATTTTGCGGc < 1:1050185/69‑1 (MQ=255)
cccAGCTCGTAGTTGTTACCGGTGATTGGAGCCAGATATTTGCCTGAACTGTCACGATCATTTTGCGGc < 1:3411348/69‑1 (MQ=255)
cccAGCTCGTAGTTGTTACCGGTGATTGGAGCCAGATATTTGCCTGAACTGTCACGATCATTTTGCGGc < 1:2794004/69‑1 (MQ=255)
cccAGCTCGTAGTTGTTACCGGTGATTGGAGCCAGATATTTGCCTGAACTGTCACGATCATTTTGCGGc < 1:3402095/69‑1 (MQ=255)
cccAGCTCGTAGTTGTTACCGGTGATTGGAGCCAGATATTTGCCTGAACTGTCACGATCATTTTGCGGc < 1:2790096/69‑1 (MQ=255)
cccAGCTCGTAGTTGTTACCGGTGATTGGAGCCAGATATTTGCCTGAACTGTCACGATCATTTTGCGGc < 1:1316827/69‑1 (MQ=255)
cTCGTAGTTGTTACCGGTGATTGGAGCCAGATATTTGCCTGAACTGTCACGATCATTTTGCGGCTGGaa < 1:21686/69‑1 (MQ=255)
cGTAGTTGTTACCGGTGATTGGAGCCAGATATTTGGCTGa < 1:3316653/40‑1 (MQ=255)
cGTAGTTGTTACCGGTGATTGGAGCCAGATATTTGCCTGa < 1:1570544/40‑1 (MQ=255)
cGTAGTTGTTACCGGTGATTGGAGCCAGATATTTGCCTGa < 1:3004109/40‑1 (MQ=255)
cGTAGTTGTTACCGGTGATTGGAGCCAGATATTTGCCTGa < 1:709036/40‑1 (MQ=255)
aGTTGTTACCGGTGATTGGAGCCAGATATTTGCCTGAACTGTCACGATCATTTTGCGGCTGGAAAATat < 1:1776899/69‑2 (MQ=255)
aGTTGTTACCGGTGATTGGAGCCAGATATTTGCCTGAACTGTCACGATCATTTTGCGGCTGGAAAATag < 1:2254841/69‑1 (MQ=255)
aGTTGTTACCGGTGATTGGAGCCAGATATTTGCCTGAACTGTCACGATCATTTTGCGGCTGGAAAATag < 1:1120393/69‑1 (MQ=255)
aGTTGTTACCGGTGATTGGAGCCAGATATTTGCCTGAACTGTCACGATCATTTTGCGGCTGGAAAATag < 1:2611423/69‑1 (MQ=255)
aGTTGTTACCGGTGATTGGAGCCAGATATTTGCCTGAACTGTCACGATCATTTTGCGGCTGGAAAATag < 1:2654630/69‑1 (MQ=255)
aGTTGTTACCGGTGATTGGAGCCAGATATTTGCCTGAACTGTCACGATCATTTTGCGGCTGGAAAATag < 1:711988/69‑1 (MQ=255)
aGTTGTTACCGGTGATTGGAGCCAGATATTTGCCTGAACTGTCACGATCATTTTGCGGCTGGAAAATag < 1:1366789/69‑1 (MQ=255)
aGTTGTTACCGGTGATTGGAGCCAGATATTTGCCTGAACTGTCACGATCATTTTGCGGCTGGAAAATag < 1:348450/69‑1 (MQ=255)
aGTTGTTACCGGTGATTGGAGCCAGATATTTGCCTGAACTGTCACGATCATTTTGCGGCTGGAAAATa < 1:1032852/68‑1 (MQ=255)
gttgttACCGGTGATTGGAGCCAGATATTTGCCTGAACTGTCACGATCATTTTGCGGCTGGAAAATag < 1:2746927/68‑1 (MQ=255)
tgttACCGGTGATTGGAGCCAGATATTTGCCTGAACTGTCACGATCATTTTGCGGCTGGAAAATAGAgg < 1:3000209/69‑1 (MQ=255)
tgttACCGGTGATTGGAGCCAGATATTTGCCTGAACTGTCACGATCATTTTGCGGCTGGAAAATAGAgg < 1:13221/69‑1 (MQ=255)
tgttACCGGTGATTGGAGCCAGATATTTGCCTGAACTGTCACGATCATTTTGCGGCTGGAAAATAGAgg < 1:1375689/69‑1 (MQ=255)
tgttACCGGTGATTGGAGCCAGATATTTGCCTGAACTGTCACGATCATTTTGCGGCTGGAAAATAGAgg < 1:3430374/69‑1 (MQ=255)
tgttACCGGTGATTGGAGCCAGATATTTGCCTGAACTGTCACGATCATTTTGCGGCTGGAAAATAGAgg < 1:2817257/69‑1 (MQ=255)
tgttACCGGTGATTGGAGCCAGATATTTGCCTGAACTGTCACGATCATTTTGCGGCTGGAAAATAGAgg < 1:618457/69‑1 (MQ=255)
tgttACCGGTGATTGGAGCCAGATATTTGCCTGAACTGTCACGATCATTTTGCGGCTGGAAAATAGAgg < 1:846970/69‑1 (MQ=255)
tgttACCGGTGATTGGAGCCAGATATTTGCCTGAACTGTCACGATCATTTTGCGGCTGGAAAATAGAgg < 1:2489577/69‑1 (MQ=255)
gttACCGGTGATTGGAGCCAGATATTTGCCTGAACTGTCACGATCATTTTGCGGCTGGAAAATAGAgg > 1:3393086/1‑68 (MQ=255)
gttACCGGTGATTGGAGCCAGATATTTGCCTGAACTGTCACGATCATTTTGCGGCTGGAAAATAGAgg > 1:1232695/1‑68 (MQ=255)
gttACCGGTGATTGGAGCCAGATATTTGCCTGAACTGTCACGATCATTTTGCGGCTGGAAAATAGAgg > 1:2095748/1‑68 (MQ=255)
gttACCGGTGATTGGAGCCAGATATTTGCCTGAACTGTCACGATCATTTTGCGGCTGGAAAATAGAgg > 1:252781/1‑68 (MQ=255)
gttACCGGTGATTGGAGCCAGATATTTGCCTGAACTGTCACGATCATTTTGCGGCTGGAAAATAGAgg > 1:2556158/1‑68 (MQ=255)
gttACCGGTGATTGGAGCCAGATATTTGCCTGAACTGTCACGATCATTTTGCGGCTGGAAAATAGAgg > 1:1793371/1‑68 (MQ=255)
gttACCGGTGATTGGAGCCAGATATTTGCCTGAACTGTCACGATCATTTTGCGGCTGGAAAATAGAgg > 1:2560110/1‑68 (MQ=255)
aCCGGTGATTGGAGCCAGATATTTGCCTGAACTGTCACGATCATTTTGCGGCTGGAAAATAGAGGtat < 1:1531837/68‑1 (MQ=255)
aCCGGTGATTGGAGCCAGATATTTGCCTGAACTGTCACGATCATTTTGCGGCTGGAAAATAGAGGtat < 1:3323238/68‑1 (MQ=255)
ggTGATTGGAGCCAGATATTTGCCTGAACTGTCACGATCATTTTGCGGCTGGAAAATAGAGGTGTAGCt < 1:2908996/69‑1 (MQ=255)
gTGATTGGAGCCAGATATTTGCCTGAACTGTCACGATCATTTTGCGGc < 1:1079661/48‑1 (MQ=255)
gATTGGAGTCAGATATTTGCCTGAACTGTCACGATCATTTTGCGGCTGGAAAATAGAGGTATAGCTGGc < 1:2716324/69‑1 (MQ=255)
gATTGGAGTCAGATATTTGCCTGAACTGTCACGATCATTTTGCGGCTGGAAAATAGAGGTATAGCTGGc < 1:2862964/69‑1 (MQ=255)
gATTGGAGTCAGATATTTGCCTGAACTGTCACGATCATTTTGCGGCTGGAAAATAGAGGTATAGCTGGc < 1:3036523/69‑1 (MQ=255)
gATTGGAGTCAGATATTTGCCTGAACTGTCACGATCATTTTGCGGCTGGAAAATAGAGGTATAGCTGGc < 1:1833541/69‑1 (MQ=255)
gATTGGAGTCAGATATTTGCCTGAACTGTCACGATCATTTTGCGGCTGGAAAATAGAGGTATAGCTGGc < 1:199973/69‑1 (MQ=255)
gATTGGAGTCAGATATTTGCCTGAACTGTCACGATCATTTTGCGGCTGGAAAATAGAGGTATAGCTGGc < 1:128798/69‑1 (MQ=255)
gATTGGAGTCAGATATTTGCCTGAACTGTCACGATCATTTTGCGGCTGGAAAATAGAGGTATAGCTGGc < 1:844346/69‑1 (MQ=255)
gATTGGAGTCAGATATTTGCCTGAACTGTCACGATCATTTTGCGGCTGGAAAATAGAGGTATAGCTGGc < 1:1231105/69‑1 (MQ=255)
gATTGGAGTCAGATATTTGCCTGAACTGTCACGATCATTTTGCGGCTGGAAAATAGAGGTATAGCTGGc < 1:230880/69‑1 (MQ=255)
gATTGGAGTCAGATATTTGCCTGAACTGTCACGATCATTTTGCGGCTGGAAAATAGAGGTATAGCTGGc < 1:326492/69‑1 (MQ=255)
gATTGGAGTCAGATATTTGCCTGAACTGTCACGATCATTTTGCGGCTGGAAAATAGAGGTATAGCTGGc < 1:3116025/69‑1 (MQ=255)
gATTGGAGTCAGATATTTGCCTGAACTGTCACGATCATTTTGCGGCTGGAAAATAGAGGTATAGCTGGc < 1:1441403/69‑1 (MQ=255)
gATTGGAGTCAGATATTTGCCTGAACTGTCACGATCATTTTGCGGCTGGAAAATAGAGGTATAGCTGGc < 1:1452347/69‑1 (MQ=255)
gATTGGAGTCAGATATTTGCCTGAACTGTCACGATCATTTTGCGGCTGGAAAATAGAGGTATAGCTGGc < 1:324977/69‑1 (MQ=255)
gATTGGAGTCAGATATTTGCCTGAACTGTCACGATCATTTTGCGGCTGGAAAATAGAGGTATAGCTGGc < 1:3271228/69‑1 (MQ=255)
gATTGGAGTCAGATATTTGCCTGAACTGTCACGATCATTTTGCGGCTGGAAAATAGAGGTATAGCTGGc < 1:3331189/69‑1 (MQ=255)
gATTGGAGTCAGATATTTGCCTGAACTGTCACGATCATTTTGCGGCTGGAAAATAGAGGTATAGCTGGc < 1:15938/69‑1 (MQ=255)
gATTGGAGTCAGATATTTGCCTGAACTGTCACGATCATTTTGCGGCTGGAAAATAGAGGTATAGCTGGc < 1:3362653/69‑1 (MQ=255)
gATTGGAGTCAGATATTTGCCTGAACTGTCACGATCATTTTGCGGCTGGAAAATAGAGGTATAGCTGGc < 1:3371959/69‑1 (MQ=255)
gATTGGAGGCAGATATTTGCCTGAACTGTCACGATCATTTTGCGGCTGGAAAATAGAGGGATAGCTGGc < 1:2847131/69‑1 (MQ=255)
aTTGGAGTCAGATATTTGCCTGAACTGTCACGATCATTTTGCGGCTGGAAAATAGAGGTATAGCTGGc < 1:1616778/68‑1 (MQ=255)
aTTGGAGTCAGATATTTGCCTGAACTGTCACGATCATTTTGCGGCTGGAAAATAGAGGTATAGCTGGc < 1:3425790/68‑1 (MQ=255)
aTTGGAGCCAGATATTTGCCTGAACTGTCACGATCATTTTGCGGCTGGAAAATAGAGGTATAGCTgg > 1:420801/1‑67 (MQ=255)
aTTGGAGCCAGATATTTGCCTGAACTGTCACGATCATTTTGCGGCTGGAAAATAGAGGTATAGCTgg > 1:1436130/1‑67 (MQ=255)
aTTGGAGCCAGATATTTGCCTGAACTGTCACGATCATTTTGCGGCTGGAAAATAGAGGTATAGCTgg > 1:863512/1‑67 (MQ=255)
aTTGGAGCCAGATATTTGCCTGAACTGTCACGATCATTTTGCGGCTGGAAAATAGAGGTATAGCTgg > 1:918262/1‑67 (MQ=255)
aTTGGAGCCAGATATTTGCCTGAACTGTCACGATCATTTTGCGGCTGGAAAATAGAGGTATAGCTgg > 1:3150560/1‑67 (MQ=255)
aTTGGAGCCAGATATTTGCCTGAACTGTCACGATCATTTTGCGGCTGGAAAATAGAGGTATAGCTgg > 1:951958/1‑67 (MQ=255)
|
CAGACGGCTATTCATCCAGTCCGATTTCAGACCCAGCTCGTAGTTGTTACCGGTGATTGGAGCCAGATATTTGCCTGAACTGTCACGATCATTTTGCGGCTGGAAAATAGAGGTATAGCTGGC > minE/739157‑739279
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
