Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F1 I0 R1
|
4577 |
57.4 |
3436498 |
77.7% |
2670158 |
63.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
744,026 |
G→C |
38.6% |
G226A (GGT→GCT) |
nagZ → |
beta N‑acetyl‑glucosaminidase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 744,026 | 0 | G | C | 38.6%
| 38.0
/ 48.7
| 57 | G226A (GGT→GCT) | nagZ | beta N‑acetyl‑glucosaminidase |
| Reads supporting (aligned to +/- strand): ref base G (29/6); new base C (9/13); total (38/19) |
| Fisher's exact test for biased strand distribution p-value = 1.61e-03 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
AAATAAACTCGACGCCATTATGCCTGCGCATGTGATCTACAGTGATGTTGATCCGCGTCCGGCGAGCGGTTCTCCCTACTGGCTGAAAACCGTTTTGCGTCAGGAACTGGGTTTTGA > minE/743958‑744074
|
aaaTAAACTCGACGCCATTATGCCTGCGCATGTGATCTACAGTGATGTTGATCCGCGTCCGGCGAGCgg < 1:1052595/69‑1 (MQ=255)
aaaTAAACTCGACGCCATTATGCCTGCGCATGTGATCTACAGTGATGTTGATCCGCGTCCGGCGAGCgg < 1:2038634/69‑1 (MQ=255)
aaaTAAACTCGACGCCATTATGCCTGCGCATGTGATCTACAGTGATGTTGATCCGCGTCCGGCGAGCgg < 1:581824/69‑1 (MQ=255)
aaaTAAACTCGACGCCATTATGCCTGCGCATGTGATCTACAGTGATGTTGATCCGCGTCCGGCGAGCgg < 1:2555192/69‑1 (MQ=255)
aaaTAAACTCGACGCCATTATGCCTGCGCATGTGATCTACAGTGATGTTGATCCGCGTCCGGCGAGCgg < 1:498184/69‑1 (MQ=255)
aTAAACTCGACTCCATTATGCCTGCGCATGTGATCTACAGTGATGTTGATCCGCGTCCGGCGAGCgg < 1:2657376/67‑1 (MQ=255)
cTCGACGCCATTATGCCTGCGCATGTGATCTACAGTGATGTTGATCCGCGTCCGGCGAGCgg < 1:143267/62‑1 (MQ=255)
cTCGACGCCATTATGCCTGCGCATGTGATCTACAGTGATGTTGATCCGCGTCCGGCGAGCGGTTCTccc < 1:710391/69‑1 (MQ=255)
cTCGACGCCATTATGCCTGCGCATGTGATCTACAGTGATGTTGATCCGCGTCCGGCGAGCGGTTCTccc < 1:2687083/69‑1 (MQ=255)
aTGCCTGCGCATGTGATCTACAGTGATGTTGATCCGCGTCCGGCGAGCGGTTCTCCCTACTGGCTGaaa > 1:1537655/1‑69 (MQ=255)
aTGCCTGCGCATGTGATCTACAGTGATGTTGATCCGCGTCCGGCGAGCGGTTCTCCCTACTGGCTGaaa > 1:2110800/1‑69 (MQ=255)
aTGCCTGCGCATGTGATCTACAGTGATGTTGATCCGCGTCCGGCGAGCGGTTCTCCCTACTGGCTGaaa > 1:600667/1‑69 (MQ=255)
aTGCCTGCGCATGTGATCTACAGTGATGTTGATCCGCGTCCGGCGAGCGGTTCTCCCTACTGGCTGaaa > 1:1962219/1‑69 (MQ=255)
aTGCCTGCGCATGTGATCTACAGTGATGTTGATCCGCGTCCGGCGAGCGGTTCTCCCTACTGGCTGaaa > 1:1937982/1‑69 (MQ=255)
aTGCCTGCGCATGTGATCTACAGTGATGTTGATCCGCGTCCGGCGAGCGGTTCTCCCTACTGGCTGaaa > 1:1002015/1‑69 (MQ=255)
aTGCCTGCGCATGTGATCTACAGTGATGTTGATCCGCGTCCGGCGAGCGGTTCTCCCTACTGGCTGaaa > 1:446154/1‑69 (MQ=255)
aTGCCTGCGCATGTGATCTACAGTGATGTTGATCCGCGTCCGGCGAGCGGTTCTCCCTACTGGCTGaaa > 1:2774724/1‑69 (MQ=255)
aTGCCTGCGCATGTGATCTACAGTGATGTTGATCCGCGTCCGGCGAGCGGTTCTCCCTACTGGCTGaaa > 1:2875372/1‑69 (MQ=255)
aTGCCTGCGCATGTGATCTACAGTGATGTTGATCCGCGTCCGGCGAGCGGTTCTCCCTACTGGCTGaaa > 1:3405107/1‑69 (MQ=255)
aTGCCTGCGCATGTGATCTACAGTGATGTTGATCCGCGTCCGGCGAGCGGTTCTCCCTACTGGCTGaaa > 1:758119/1‑69 (MQ=255)
cTACAGTGATGTTGATCCGCGTCCGGCGAGCGGTTCTCCCTACTGGCTGAAAACCGTTTTGCGTCAgg < 1:871351/68‑1 (MQ=255)
cTACAGTGATGTTGATCCGCGTCCGGCGAGCGGTTCTCCCTACTGGCTGAAAACCGTTTTGCGTCAgg < 1:2839318/68‑1 (MQ=255)
cTACAGTGATGTTGATCCGCGTCCGGCGAGCGGTTCTCCCTACTGGCTGAAAACCGTTTTGCGTCAgg < 1:3286625/68‑1 (MQ=255)
cTACAGTGATGTTGATCCGCGTCCGGCGAGCGGTTCTCCCTACTGGCTGAAAACCGTTTTGCGTCAgg < 1:1479140/68‑1 (MQ=255)
cAGTGATGTTGATCCGCGTCCGGCGAGCGGTTCTCCCTACTGGCTGAAAACCGTTTTGCGTCAGGAACt > 1:2300647/1‑69 (MQ=255)
cAGTGATGTTGATCCGCGTCCGGCGAGCGGTTCTCCCTACTGGCTGAAAACCGTTTTGCGTCAGGAACt > 1:1319731/1‑69 (MQ=255)
cAGTGATGTTGATCCGCGTCCGGCGAGCGGTTCTCCCTACTGGCTGAAAACCGTTTTGCGTCAGGAACt > 1:3357488/1‑69 (MQ=255)
cAGTGATGTTGATCCGCGTCCGGCGAGCGGTTCTCCCTACTGGCTGAAAACCGTTTTGCGTCAGGAACt > 1:1759735/1‑69 (MQ=255)
cAGTGATGTTGATCCGCGTCCGGCGAGCGGTTCTCCCTACTGGCTGAAAACCGTTTTGCGTCAGGAACt > 1:1516556/1‑69 (MQ=255)
gTGATGTTGATCCGCGTCCGGCGAGCGCTTCTCCCTACTGGCTGAAAGCCGTTTTGCGTCAGGaa < 1:656377/65‑1 (MQ=255)
gTGATGTTGATCCGCGTCCGGCGAGCGCTTCTCCCTACTGGCTGAAAGCCGTTTTGCGTCAGGaa < 1:3399937/65‑1 (MQ=255)
gTGATGTTGATCCGCGTCCGGCGAGCGCTTCTCCCTACTGGCTGAAAGCCGTTTTGCGTCAGGaa < 1:3027927/65‑1 (MQ=255)
gTGATGTTGATCCGCGTCCGGCGAGCGCTTCTCCCTACTGGCTGAAAGCCGTTTTGCGTCAGGaa < 1:1421375/65‑1 (MQ=255)
gTGATGTTGATCCGCGTCCGGCGAGCGCTTCTCCCTACTGGCTGAAAGCCGTTTTGCGTCAGGaa < 1:1487598/65‑1 (MQ=255)
gTGATGTTGATCCGCGTCCGGCGAGCGCTTCTCCCTACTGGCTGAAAGCCGTTTTGCGTCAGGaa < 1:1621789/65‑1 (MQ=255)
gTGATGTTGATCCGCGTCCGGCGAGCGCTTCTCCCTACTGGCTGAAAGCCGTTTTGCGTCAGGaa < 1:670702/65‑1 (MQ=255)
gTGATGTTGATCCGCGTCCGGCGAGCGCTTCTCCCTACTGGCTGAAAGCCGTTTTGCGTCAGGaa < 1:637812/65‑1 (MQ=255)
gTGATGTTGATCCGCGTCCGGCGAGCGCTTCTCCCTACTGGCTGAAAGCCGTTTTGCGTCAGGaa < 1:201991/65‑1 (MQ=255)
gTGATGTTGATCCGCGTCCGGCGAGCGCTTCTCCCTACTGGCTGAAAGCCGTTTTGCGTCAGGaa < 1:203900/65‑1 (MQ=255)
gTGATGTTGATCCGCGTCCGGCGAGCGCTTCTCCCTACTGGCTGAAAGCCGTTTTGCGTCAGGaa < 1:2341893/65‑1 (MQ=255)
gTGATGTTGATCCGCGTCCGGCGAGCGCTTCTCCCTACTGGCTGAAAGCCGTTTTGCGTCAGGaa < 1:2083788/65‑1 (MQ=255)
gTGATGTTGATCCGCGTCCGGCGAGCGCTTCTCCCTAATGGCTGAAAGCCGTTTTGCGTCAGGaa < 1:62069/65‑1 (MQ=255)
aTGTTGATCCGCGTCCGGCGAGCGGTTCTCCCTACTGGCTGAAAACCGTTTTGCGTCAGGAACTGGGtt > 1:57566/1‑69 (MQ=255)
aTGTTGATCCGCGTCCGGCGAGCGGTTCTCCCTACTGGCTGAAAACCGTTTTGCGTCAGGAACTGGGtt > 1:3311075/1‑69 (MQ=255)
aTGTTGATCCGCGTCCGGCGAGCGGTTCTCCCTACTGGCTGAAAACCGTTTTGCGTCAGGAACTGGGtt > 1:2604424/1‑69 (MQ=255)
aTGTTGATCCGCGTCCGGCGAGCGGTTCTCCCTACTGGCTGAAAACCGTTTTGCGTCAGGAACTGGGtt > 1:2465361/1‑69 (MQ=255)
aTGTTGATCCGCGTCCGGCGAGCGGTTCTCCCTACTGGCTGAAAACCGTTTTGCGTCAGGAACTGGGtt > 1:1040451/1‑69 (MQ=255)
aTGTTGATCCGCGTCCGGCGAGCGGTTCTCCCTAATGGCTGAAAACCGTTTTGCGTCAGGAACTGGGtt > 1:1646673/1‑69 (MQ=255)
ttGATGCGCGTCCGGCGAGCGGTTCTCACTACTGGCTGAAAACCGTTTTGCGTCAGGAACTGGGTTTTg > 1:2590086/1‑69 (MQ=255)
ttGATCCGCGTCCGGCGAGCGGTTCTCCCTACTGGCTGAAAACCGTTTTGCGTCAGGAACTGGGTTTTg > 1:3387991/1‑69 (MQ=255)
ttGATCCGCGTCCGGCGAGCGGTTCTCCCTACTGGCTGAAAACCGTTTTGCGTCAGGAACTGGGTTTTg > 1:306386/1‑69 (MQ=255)
ttGATCCGCGTCCGGCGAGCGGTTCTCCCTACTGGCTGAAAACCGTTTTGCGTCAGGAACTGGGTTTTg > 1:855217/1‑69 (MQ=255)
ttGATCCGCGTCCGGCGAGCGGTTCTCCCTACTGGCTGAAAACCGTTTTGCGTCAGGAACTGGGTTTTg > 1:1511340/1‑69 (MQ=255)
ttGATCCGCGTCCGGCGAGCGGTTCTCCCTACTGGCTGAAAACCGTTTTGCGTCAGGAACTGGGTTTTg > 1:3257963/1‑69 (MQ=255)
ttGATCCGCGTCCGGCGAGCGGTTCTCCCTACTGGCTGAAAACCGTTTTGCGTCAGGAACTGGGTTTTg > 1:2150274/1‑69 (MQ=255)
tGATCCGCGTCCGGCGAGCGCTTCTCCCTACTGGCTGAAAGCCGTTTTGCGTCAGGAATTGGGTTTTGa > 1:313918/1‑69 (MQ=255)
tGATCCGCGTCCGGCGAGCGCTTCTCCCTACTGGCTGAAAGCCGTTTTGCGTCAGGAATTGGGTTTTGa > 1:1110897/1‑69 (MQ=255)
tGATCCGCGTCCGGCGAGCGCTTCTCCCTACTGGCTGAAAGCCGTTTTGCGTCAGGAATTGGGTTTTGa > 1:1638043/1‑69 (MQ=255)
tGATCCGCGTCCGGCGAGCGCTTCTCCCTACTGGCTGAAAGCCGTTTTGCGTCAGGAATTGGGTTTTGa > 1:1751925/1‑69 (MQ=255)
tGATCCGCGTCCGGCGAGCGCTTCTCCCTACTGGCTGAAAGCCGTTTTGCGTCAGGAATTGGGTTTTGa > 1:235777/1‑69 (MQ=255)
tGATCCGCGTCCGGCGAGCGCTTCTCCCTACTGGCTGAAAGCCGTTTTGCGTCAGGAATTGGGTTTTGa > 1:2493275/1‑69 (MQ=255)
tGATCCGCGTCCGGCGAGCGCTTCTCCCTACTGGCTGAAAGCCGTTTTGCGTCAGGAATTGGGTTTTGa > 1:2663203/1‑69 (MQ=255)
tGATCCGCGTCCGGCGAGCGCTTCTCCCTACTGGCTGAAAGCCGTTTTGCGTCAGGAATTGGGTTTTGa > 1:3027576/1‑69 (MQ=255)
tGATCCGCGTCCGGCGAGCGCTTCTCCCTACTGGCTGAAAGCCGTTTTGCGTCAGGAATTGGGGTTTGa > 1:3112835/1‑69 (MQ=255)
|
AAATAAACTCGACGCCATTATGCCTGCGCATGTGATCTACAGTGATGTTGATCCGCGTCCGGCGAGCGGTTCTCCCTACTGGCTGAAAACCGTTTTGCGTCAGGAACTGGGTTTTGA > minE/743958‑744074
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
