Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A2 F1 I0 R1 4577 57.4 3436498 77.7% 2670158 63.4

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 761,758 A→G 24.4% N109N (AAT→AAC)  potD ← polyamine transporter subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE761,7580AG24.4% 114.3 / 32.5 94N109N (AAT→AAC) potDpolyamine transporter subunit
Reads supporting (aligned to +/- strand):  ref base A (34/37);  new base G (12/11);  total (46/48)
Fisher's exact test for biased strand distribution p-value = 8.12e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.80e-01

Only 100 of 105 total aligned reads displayed.

ATACGGAATGGAGTAGTCGTTATTCGGGTCAAAAGGCTTGTTGAGCATGTCTGGATCGAGATTGCTGAAGTTTGTTAACTTCGACTTGTCGATCTTCTGGATCATCCCTTCTTTACGCATTTTATCAA  >  minE/761698‑761825
                                                            |                                                                   
ataCGGAATGGAGTAGTCGTTATTCGGGTCAAAAGGCTTGTTGAGCATGTCTGGATCGAGATTGCTGaa                                                             >  1:2326747/1‑69 (MQ=255)
   cGGAATGGAGTAGTCGTTATTCGGGTCAAAAGGCTTGTTGAGCATGTCTGGATCGAGATTGc                                                                 >  1:2105395/1‑62 (MQ=255)
   cGGAATGGAGTAGTCGTTATTCGGGTCAAAAGGCTTGTTGAGCATGTCTGGATCGAGATTGc                                                                 >  1:2847574/1‑62 (MQ=255)
   cGGAATGGAGTAGTCGTTATTCGGGTCAAAAGGCTTGTTGAGCATGTCTGGATCGAGATTGCTGAAGtt                                                          >  1:209892/1‑69 (MQ=255)
   cGGAATGGAGTAGTCGTTATTCGGGTCAAAAGGCTTGTTGAGCATGTCTGGATCGAGATTGCTGAAGtt                                                          >  1:2657949/1‑69 (MQ=255)
   cGGAATGGAGTAGTCGTTATTCGGGTCAAAAGGCTTGTTGAGCATGTCTGGATCGAGATTGCTGAAGtt                                                          >  1:2861902/1‑69 (MQ=255)
   cGGAATGGAGTAGTCGTTATTCGGGTCAAAAGGCTTGTTGAGCATGTCTGGATCGAGATTGCTGAAGtt                                                          >  1:165339/1‑69 (MQ=255)
   cGGAATGGAGTAGTCGTTATTCGGGTCAAAAGGCTTGTTGAGCATGTCTGGATCGAGATTGCTGAAGtt                                                          >  1:2303452/1‑69 (MQ=255)
   cGGAATGGAGTAGTCGTTATTCGGGTCAAAAGGCTTGTTGAGCATGTCTGGATCGAGATTGCTGAAGtt                                                          >  1:3119929/1‑69 (MQ=255)
   cGGAATGGAGTAGTCGTTATTCGGGTCAAAAGGCTTGTTGAGCATGTCTGGATCGAGATTGCTGAAGtt                                                          >  1:3299474/1‑69 (MQ=255)
   cGGAATGGAGTAGTCGTTATTCGGGTCAAAAGGCTTGTTGAGCATGTCTGGATCGAGATTGCTGAAGtt                                                          >  1:1732132/1‑69 (MQ=255)
   cGGAATGGAGTAGTCGTTATTCGGGTCAAAAGGCTTGTTGAGCATGTCTGGATCGAGATTGCTGAAGt                                                           >  1:628967/1‑68 (MQ=255)
   cGGAATGGAGTAGTCGTTATTCGGGTCAAAAGGCTTGTTGAGCATGTATGGATCGAGATTGCTGAAGtt                                                          >  1:360349/1‑69 (MQ=255)
   cGGAATGGAGTAGTCGTTATTCGGGTCAAAAGGCTTGTTGAGCATGACTGGATCGAGATTGCTGAAGtt                                                          >  1:3422211/1‑69 (MQ=255)
             tagtCGTTATTCGGGTCAAAAGGCTTGTTGAGCATGTCTGGATCGAGATTGCTGAAGTTTGTTAACTTc                                                <  1:1317799/69‑1 (MQ=255)
             tagtCGTTATTCGGGTCAAAAGGCTTGTTGAGCATGTCTGGATCGAGATTGCTGAAGTTTGTTAACTTc                                                <  1:3026476/69‑1 (MQ=255)
             tagtCGTTATTCGGGTCAAAAGGCTTGTTGAGCATGTCTGGATCGAGATTGCTGAAGTTTGTTAACTTc                                                <  1:1368355/69‑1 (MQ=255)
               gtCGTTATTCGGGTCAAAAGGCTTGTTGAGCATGTCTGGATCGAGATTGCTGAAGTTTGTTAACTTCGa                                              >  1:2224168/1‑69 (MQ=255)
               gtCGTTATTCGGGTCAAAAGGCTTGTTGAGCATGTCTGGATCGAGATTGCTGAAGTTTGTTAACTTCGa                                              <  1:2189237/69‑1 (MQ=255)
               gtCGTTATTCGGGTCAAAAGGCTTGTTGAGCATGTCTGGATCGAGATTGCTGAAGTTTGTTAACTTCGa                                              >  1:2955516/1‑69 (MQ=255)
               gtCGTTATTCGGGTCAAAAGGCTTGTTGAGCATGTCTGGATCGAGATTGCTGAAGTTTGTTAACTTCGa                                              >  1:2537871/1‑69 (MQ=255)
               gtCGTTATTCGGGTCAAAAGGCTTGTTGAGCATGTCTGGATCGAGATTGCTGAAGTTTGTTAACTTCGa                                              >  1:2584461/1‑69 (MQ=255)
               gtCGTTATTCGGGTCAAAAGGCTTGTTGAGCATGTCTGGATCGAGATTGCTGAAGTTTGTTAACTTCGa                                              >  1:2602920/1‑69 (MQ=255)
               gtCGTTATTCGGGTCAAAAGGCTTGTTGAGCATGTCTGGATCGAGATTGCTGAAGTTTGTTAACTTCGa                                              <  1:2925035/69‑1 (MQ=255)
               gtCGTTATTCGGGTCAAAAGGCTTGTTGAGCATGTCTGGATCGAGATTGCTGAAGTTTGTTAACTTCGa                                              <  1:2482938/69‑1 (MQ=255)
               gtCGTTATTCGGGTCAAAAGGCTTGTTGAGCATGTCTGGATCGAGATTGCTGAAGTTTGTTAACTTCGa                                              >  1:3027859/1‑69 (MQ=255)
               gtCGTTATTCGGGTCAAAAGGCTTGTTGAGCATGTCTGGATCGAGATTGCTGAAGTTTGTTAACTTCGa                                              <  1:3168255/69‑1 (MQ=255)
               gtCGTTATTCGGGTCAAAAGGCTTGTTGAGCATGTCTGGATCGAGATTGCTGAAGTTTGTTAACTTCGa                                              <  1:623926/69‑1 (MQ=255)
               gtCGTTATTCGGGTCAAAAGGCTTGTTGAGCATGTCTGGATCGAGATTGCTGAAGTTTGTTAACTTCGa                                              >  1:825383/1‑69 (MQ=255)
               gtCGTTATTCGGGTCAAAAGGCTTGTTGAGCATGTCTGGATCGAGATTGCTGAAGTTTGTTAACTTCGa                                              >  1:870117/1‑69 (MQ=255)
               gtCGTTATTCGGGTCAAAAGGCTTGTTGAGCATGTCTGGATCGAGATTGCTGAAGTTTGTTAACTTCGa                                              >  1:908845/1‑69 (MQ=255)
               gtCGTTATTCGGGTCAAAAGGCTTGTTGAGCATGTCTGGATCGAGATTGCTGAAGTTTGTTAACTTCGa                                              <  1:1102860/69‑1 (MQ=255)
               gtCGTTATTCGGGTCAAAAGGCTTGTTGAGCATGTCTGGATCGAGATTGCTGAAGTTTGTTAACTTCGa                                              <  1:1164297/69‑1 (MQ=255)
               gtCGTTATTCGGGTCAAAAGGCTTGTTGAGCATGTCTGGATCGAGATTGCTGAAGTTTGTTAACTTCGa                                              >  1:1710143/1‑69 (MQ=255)
               gtCGTTATTCGGGTCAAAAGGCTTGTTGAGCATGTCTGGATCGAGATTGCTGAAGTTTGTTAACTTCGa                                              >  1:1387812/1‑69 (MQ=255)
               gtCGTTATTCGGGTCAAAAGGCTTGTTGAGCATGTCTGGATCGAGATTGCTGAAGTTTGTTAACTTCGa                                              >  1:1750513/1‑69 (MQ=255)
               gtCGTTATTCGGGTCAAAAGGCTTGTTGAGCATGTCTGGATCGAGATTGCTGAAGTTTGTTAACTTCGa                                              >  1:1383924/1‑69 (MQ=255)
               gtCGTTATTCGGGTCAAAAGGCTTGTTGAGCATGTCTGGATCGAGATTGCTGAAGTTTGTTAACTTCGa                                              <  1:1278815/69‑1 (MQ=255)
               gtCGTTATTCGGGTCAAAAGGCTTGTTGAGCATGTCTGGATCGAGATTGCTGAACTTTGTTAACTTCGa                                              >  1:47423/1‑69 (MQ=255)
               gtCGTTATTCGGGTCAAAAGGCTTGTTGAGCATGTCT‑GATCGAGATTGCTGAAGTTTGTTAACtt                                                 >  1:1233588/1‑65 (MQ=255)
                  gTTATTCGGGTCAAAAGGCTTGTTGAGCATGTCTGGATCGAGATTGCTGAAGTTTGTTAACTTCGACtt                                           >  1:2937574/1‑69 (MQ=255)
                    tATTCGGGTCAAACGGCTTGTTGAGCATGTCTGGATCGAGGTTGCTGAAATTGCTTAACTTCGACTTg                                          >  1:2975767/1‑68 (MQ=255)
                    tATTCGGGTCAAACGGCTTGTTGAGCATGTCTGGATCGAGGTTGCTGAAATTGCTTAACTTCGACTTg                                          >  1:1358238/1‑68 (MQ=255)
                    tATTCGGGTCAAACGGCTTGTTGAGCATGTCTGGATCGAGGTTGCTGAAATTGCTTAACTTCGACTTg                                          >  1:2941695/1‑68 (MQ=255)
                    tATTCGGGTCAAACGGCTTGTTGAGCATGTCTGGATCGAGGTTGCTGAAATTGCTTAACTTCGACTTg                                          >  1:1479742/1‑68 (MQ=255)
                    tATTCGGGTCAAACGGCTTGTTGAGCATGTCTGGATCGAGGTTGCTGAAATTGCTTAACTTCGACTTg                                          >  1:3247060/1‑68 (MQ=255)
                    tATTCGGGTCAAACGGCTTGTTGAGCATGTCTGGATCGAGGTTGCTGAAATTGCTTAACTTCGACTTg                                          >  1:3281035/1‑68 (MQ=255)
                    tATTCGGGTCAAACGGCTTGTTGAGCATGTCTGGATCGAGGTTGCTGAAATTGCTTAACTTCGACTTg                                          >  1:2369511/1‑68 (MQ=255)
                    tATTCGGGTCAAACGGCTTGTTGAGCATGTCTGGATCGAGGTTGCTGAAATTGCTTAACTTCGACTTg                                          >  1:8046/1‑68 (MQ=255)
                    tATTCGGGTCAAACGGCTTGTTGAGCATGTCTGGATCGAGGTTGCTGAAATTGCTTAACTTCGACTTg                                          >  1:1608403/1‑68 (MQ=255)
                    tATTCGGGTCAAACGGCTTGTTGAGCATGTCTGGATCGAGGTTGCTGAAATTGCTTAACTTCGACTTg                                          >  1:2158540/1‑68 (MQ=255)
                    tATTCGGGTCAAACGGCTTGTTGAGCATGTCTGGATCGAGGTTGCTGAAATTGCTTAACTTCGACTTg                                          >  1:1738296/1‑68 (MQ=255)
                     aTTCGGGTCAAACGGCTTGTTGAGCATGTCTGGATCGAGGTTGCTGAAATTGCTTAACTTCGACTTGTc                                        <  1:3282052/69‑1 (MQ=255)
                     aTTCGGGTCAAACGGCTTGTTGAGCATGTCTGGATCGAGGTTGCTGAAATTGCTTAACTTCGACTTGTc                                        <  1:3424297/69‑1 (MQ=255)
                     aTTCGGGTCAAACGGCTTGTTGAGCATGTCTGGATCGAGGTTGCTGAAATTGCTTAACTTCGACTTGTc                                        <  1:3434855/69‑1 (MQ=255)
                     aTTCGGGTCAAACGGCTTGTTGAGCATGTCTGGATCGAGGTTGCTGAAATTGCTTAACTTCGACTTGTc                                        <  1:2802177/69‑1 (MQ=255)
                     aTTCGGGTCAAACGGCTTGTTGAGCATGTCTGGATCGAGGTTGCTGAAATTGCTTAACTTCGACTTGTc                                        <  1:1189094/69‑1 (MQ=255)
                     aTTCGGGTCAAACGGCTTGTTGAGCATGTCTGGATCGAGGTTGCTGAAATTGCTTAACTTCGACTTGTc                                        <  1:1513831/69‑1 (MQ=255)
                     aTTCGGGTCAAACGGCTTGTTGAGCATGTCTGGATCGAGGTTGCTGAAATTGCTTAACTTCGACTTGTc                                        <  1:1597225/69‑1 (MQ=255)
                     aTTCGGGTCAAACGGCTTGTTGAGCATGTCTGGATCGAGGTTGCTGAAATTGCTTAACTTCGACTTGTc                                        <  1:1020402/69‑1 (MQ=255)
                     aTTCGGGTCAAACGGCTTGTTGAGCATGTCTGGATCGAGGTTGCTGAAATTGCTTAACTTCGACTTGTc                                        <  1:2377084/69‑1 (MQ=255)
                     aTTCGGGTCAAAAGGCTTGTTGAGCATGTCTGGATCGAGATTGCTGAAGTTTGTTAACTTCGACTTGTc                                        >  1:367988/1‑69 (MQ=255)
                     aTTCGGGTCAAAAGGCTTGTTGAGCATGTCTGGATCGAGATTGCTGAAGTTTGTTAACTTCGACTTGTc                                        >  1:1837535/1‑69 (MQ=255)
                     aTTCGGGTCAAAAGGCTTGTTGAGCATGTCTGGATCGAGATTGCTGAAGTTTGTTAACTTCGACTTGTc                                        >  1:1262362/1‑69 (MQ=255)
                                   gCTTGTTGATCATGTCTGGATCGAGATTGCTGAAGTTTGTTAACTTCGACTTGTCGATCTTCTGGatca                          <  1:2173769/69‑1 (MQ=255)
                                   gCTTGTTGAGCATGTCTGGATCGAGATTGCTGAAGTTTGTTAACTTCGACTTGTCGATCTTCTGGatca                          <  1:2197324/69‑1 (MQ=255)
                                   gCTTGTTGAGCATGTCTGGATCGAGATTGCTGAAGTTTGTTAACTTCGACTTGTCGATCTTCTGGatca                          <  1:3218071/69‑1 (MQ=255)
                                   gCTTGTTGAGCATGTCTGGATCGAGATTGCTGAAGTTTGTTAACTTCGACTTGTCGATCTTCTGGatca                          <  1:1932738/69‑1 (MQ=255)
                                   gCTTGTTGAGCATGTCTGGATCGAGATTGCTGAAGTTTGTTAACTTCGACTTGTCGATCTTCTGGatca                          <  1:1266996/69‑1 (MQ=255)
                                   gCTTGTTGAGCATGTCTGGATCGAGATTGCTGAAGTTTGTTAACTTCGACTTGTCGATCTTCTGGatca                          <  1:339074/69‑1 (MQ=255)
                                   gCTTGTTGAGCATGTCTGGATCGAGATTGCTGAAGTTTGTTAACTTCGACTTGTCGATCTTCTGGatca                          <  1:2026666/69‑1 (MQ=255)
                                   gCTTGTTGAGCATGTCTGGATCGAGATTGCTGAAGTTTGTTAACTTCGACTTGTCGATCTTCTGGatca                          <  1:2982322/69‑1 (MQ=255)
                                   gCTTGTTGAGCATGTCTGGATCGAGATTGCTGAAGTTTGTTAACTTCGACTTGTCGATCTTCTGGatca                          <  1:1591955/69‑1 (MQ=255)
                                   gCTTGTTGAGCATGTCTGGATCGAGATTGCTGAAGTTTGTTAACTTCGACTTGTCGATCTTCTGGatca                          <  1:1436369/69‑1 (MQ=255)
                                   gCTTGTTGAGCATGTCTGGATCGAGATTGCTGAAGTTTGTTAACTTCGACTTGTCGATCTTCTGGatca                          <  1:2281200/69‑1 (MQ=255)
                                         tgAGCATGTCTGGATCGAGATTGCTGAAGTTTGTTAACTTCGACTTGTCGATCTTCTGGATCATccc                      <  1:1047339/67‑1 (MQ=255)
                                         tgAGCATGTCTGGATCGAGATTGCTGAAGTTTGTTAACTTCGACTTGTCGATCTTCTGGATCATccc                      <  1:8284/67‑1 (MQ=255)
                                         tgAGCATGTCTGGATCGAGATTGCTGAAGTTTGTTAACTTCGACTTGTCGATCTTCTGGATCATccc                      <  1:669537/67‑1 (MQ=255)
                                         tgAGCATGTCTGGATCGAGATTGCTGAAGTTTGTTAACTTCGACTTGTCGATCTTCTGGATCATccc                      <  1:3233066/67‑1 (MQ=255)
                                         tgAGCATGTCTGGATCGAGATTGCTGAAGTTTGTTAACTTCGACTTGTCGATCTTCTGGATCATccc                      <  1:2054376/67‑1 (MQ=255)
                                         tgAGCATGTCTGGATCGAGATTGCTGAAGTTTGTTAACTTCGACTTGTCGATCTTCTGGATCATccc                      <  1:2955122/67‑1 (MQ=255)
                                             cATGTCTGGATCGAGATTGCTGAAGTTTGTTAACTTCGACTTGTCGATCTTCTGGATCATCCcttctt                 <  1:2031952/68‑1 (MQ=255)
                                              aTGTCTGGATCGAGATTGCTGAAGTTTGTTAACTTCGGCTTGTCGATCTTCTGGATCATCCCTTCTTTa               <  1:3367027/69‑1 (MQ=255)
                                              aTGTCTGGATCGAGATTGCTGAAGTTTGTTAACTTCGACTTGTCGATCTTCTGGATCATCCCTTCTTTa               <  1:1304521/69‑1 (MQ=255)
                                              aTGTCTGGATCGAGATTGCTGAAGTTTGTTAACTTCGACTTGTCGATCTTCTGGATCATCCCTTCTTTa               <  1:3062952/69‑1 (MQ=255)
                                              aTGTCTGGATCGAGATTGCTGAAGTTTGTTAACTTCGACTTGTCGATCTTCTGGATCATCCCTTCTTTa               <  1:3032035/69‑1 (MQ=255)
                                              aTGTCTGGATCGAGATTGCTGAAGTTTGTTAACTTCGACTTGTCGATCTTCTGGATCATCCCTTCTTTa               <  1:2665351/69‑1 (MQ=255)
                                              aTGTCTGGATCGAGATTGCTGAAGTTTGTTAACTTCGACTTGTCGATCTTCTGGATCATCCCTTCTTTa               <  1:1774301/69‑1 (MQ=255)
                                                   tGGATCGAGGTTGCTGAAATTGCTTAACTTCGACTTGTc                                        <  1:681038/39‑1 (MQ=25)
                                                           gaTTGCTGAAGTTTGTTAACTTCGACTTGTCGATCTTCTGGATCATCCCTTCTTTACGCATTTTATCaa  <  1:2911851/69‑1 (MQ=255)
                                                           gaTTGCTGAAGTTTGTTAACTTCGACTTGTCGATCTTCTGGATCATCCCTTCTTTACGCATTTTATCaa  <  1:1808225/69‑1 (MQ=255)
                                                           gaTTGCTGAAGTTTGTTAACTTCGACTTGTCGATCTTCTGGATCATCCCTTCTTTACGCATTTTATCaa  <  1:1153701/69‑1 (MQ=255)
                                                           gaTTGCTGAAGTTTGTTAACTTCGACTTGTCGATCTTCTGGATCATCCCTTCTTTACGCATTTTATCaa  <  1:1700187/69‑1 (MQ=255)
                                                           gaTTGCTGAAGTTTGTTAACTTCGACTTGTCGATCTTCTGGATCATCCCTTCTTTACGCATTTTATCaa  <  1:1491151/69‑1 (MQ=255)
                                                           gaTTGCTGAAGTTTGTTAACTTCGACTTGTCGATCTTCTGGATCATCCCTTCTTTACGCATTTTATCaa  <  1:3259014/69‑1 (MQ=255)
                                                           gaTTGCTGAAGTTTGTTAACTTCGACTTGTCGATCTTCTGGATCATCCCTTCTTTACGCATTTTATCaa  <  1:1353980/69‑1 (MQ=255)
                                                           gaTTGCTGAAGTTTGTTAACTTCGACTTGTCGATCTTCTGGATCATCCCTTCTTTACGCATTTTATCaa  <  1:342518/69‑1 (MQ=255)
                                                           gaTTGCTGAAGTTTGTTAACTTCGACTTGTCGATCTTCTGGATCATCCCTTCTTTACGCATTTTATCaa  <  1:3419579/69‑1 (MQ=255)
                                                           gaTTGCTGAAGTTTGTTAACTTCGACTTGTCGATCTTCTGGATCATCCCTTCTTTACGCATTTTATCaa  <  1:3140345/69‑1 (MQ=255)
                                                           gaTTGCGGAAGTTTGTTAACTTCGACTTGTCGATCTTCTGGATCATCCCTTCTTTACGCATTTTATCaa  <  1:1801773/69‑1 (MQ=255)
                                                            |                                                                   
ATACGGAATGGAGTAGTCGTTATTCGGGTCAAAAGGCTTGTTGAGCATGTCTGGATCGAGATTGCTGAAGTTTGTTAACTTCGACTTGTCGATCTTCTGGATCATCCCTTCTTTACGCATTTTATCAA  >  minE/761698‑761825

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A