Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A2 F1 I0 R1 4577 57.4 3436498 77.7% 2670158 63.4

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 783,256 C→T 51.5% G92G (GGC→GGT)  umuD → DNA polymerase V, subunit D

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE783,2560CT51.5% ‑3.0 / 119.7 128G92G (GGC→GGT) umuDDNA polymerase V, subunit D
Reads supporting (aligned to +/- strand):  ref base C (56/6);  new base T (24/42);  total (80/48)
Fisher's exact test for biased strand distribution p-value = 1.29e-10
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.14e-01

Only 100 of 129 total aligned reads displayed.

TTACTGATTGTCGATAGCGCTATTACCGCCAGCCATGGTGATATTGTCATCGCTGCTGTTGACGGCGAGTTTACGGTGAAAAAATTGCAACTACGCCCGACGGTACAGCTTATTCCCATGAACAGCGCGTA  >  minE/783191‑783321
                                                                 |                                                                 
ttACTGATTGTCGATAGCGCTATTGCCGCCAGCCATGGTGATATTGTCATCGCTGCTGTTGACGGCGAg                                                                >  1:2550544/1‑69 (MQ=255)
ttACTGATTGTCGATAGCGCTATTACCGCCAGCCATGGTGATATTGTCATCGCTGCTGTTGACGGCGAg                                                                >  1:345587/1‑69 (MQ=255)
ttACTGATTGTCGATAGCGCTATTACCGCCAGCCATGGTGATATTGTCATCGCTGCTGTTGACGGCGAg                                                                >  1:3136351/1‑69 (MQ=255)
ttACTGATTGTCGATAGCGCTATTACCGCCAGCCATGGTGATATTGTCATCGCTGCTGTTGACGGCGAg                                                                >  1:1593336/1‑69 (MQ=255)
ttACTGATTGTCGATAGCGCTATTACCGCCAGCCATGGTGATATTGTCATCGCTGCTGTTGACGGCGAg                                                                >  1:2678064/1‑69 (MQ=255)
ttACTGATTGTCGATAGCGCTATTACCGCCAGCCATGGTGATATTGTCATCGCTGCTGTTGACGGCGAg                                                                >  1:2556477/1‑69 (MQ=255)
ttACTGATTGTCGATAGCGCTATTACCGCCAGCCATGGTGATATTGTCATCGCTGCTGTTGACGGCGAg                                                                >  1:1683913/1‑69 (MQ=255)
ttACTGATTGTCGATAGCGCTATTACCGCCAGCCATGGTGATATTGTCATCGCTGCTGTTGACGGCGAg                                                                >  1:1852473/1‑69 (MQ=255)
        tGTCGATAGCGCTATTACCGCCAGCCATGGTGATATTGTCATCGCTGCTTTTGACGGTGAGTTTACg                                                          <  1:64699/67‑1 (MQ=255)
        tGTCGATAGCGCTATTACCGCCAGCCATGGTGATATTGTCATCGCTGCTGTTGACGGTGAGTTTACg                                                          <  1:2432772/67‑1 (MQ=255)
        tGTCGATAGCGCTATTACCGCCAGCCATGGTGATATTGTCATCGCTGCTGTTGACGGTGAGTTTACg                                                          <  1:1433487/67‑1 (MQ=255)
        tGTCGATAGCGCTATTACCGCCAGCCATGGTGATATTGTCATCGCTGCTGTTGACGGTGAGTTTACg                                                          <  1:1843100/67‑1 (MQ=255)
        tGTCGATAGCGCTATTACCGCCAGCCATGGTGATATTGTCATCGCTGCTGTTGACGGTGAGTTTACg                                                          <  1:2182606/67‑1 (MQ=255)
        tGTCGATAGCGCTATTACCGCCAGCCATGGTGATATTGTCATCGCTGCTGTTGACGGTGAGTTTACg                                                          <  1:2188408/67‑1 (MQ=255)
        tGTCGATAGCGCTATTACCGCCAGCCATGGTGATATTGTCATCGCTGCTGTTGACGGTGAGTTTACg                                                          <  1:2415617/67‑1 (MQ=255)
        tGTCGATAGCGCTATTACCGCCAGCCATGGTGATATTGTCATCGCTGCTGTTGACGGTGAGTTTACg                                                          <  1:1025438/67‑1 (MQ=255)
        tGTCGATAGCGCTATTACCGCCAGCCATGGTGATATTGTCATCGCTGCTGTTGACGGTGAGTTTACg                                                          <  1:3034246/67‑1 (MQ=255)
        tGTCGATAGCGCTATTACCGCCAGCCATGGTGATATTGTCATCGCTGCTGTTGACGGTGAGTTTACg                                                          <  1:677842/67‑1 (MQ=255)
               aGCGCTATTACCGCCAGCCATGGTGATATTGTCATCGCTGCTGTTGACGGCGAGTTTACGGTGaaaa                                                   >  1:874939/1‑67 (MQ=255)
               aGCGCTATTACCGCCAGCCATGGTGATATTGTCATCGCTGCTGTTGACGGCGAGTTTACGGTGaaaa                                                   >  1:1124945/1‑67 (MQ=255)
               aGCGCTATTACCGCCAGCCATGGTGATATTGTCATCGCTGCTGTTGACGGCGAGTTTACGGTGaaaa                                                   <  1:1565137/67‑1 (MQ=255)
               aGCGCTATTACCGCCAGCCATGGTGATATTGTCATCGCTGCTGTTGACGGCGAGTTTACGGTGaaaa                                                   <  1:1202215/67‑1 (MQ=255)
               aGCGCTATTACCGCCAGCCATGGTGATATTGTCATCGCTGCTGTTGACGGCGAGTTTACGGTGaaaa                                                   >  1:3185032/1‑67 (MQ=255)
               aGCGCTATTACCGCCAGCCATGGTGATATTGTCATCGCTGCTGTTGACGGCGAGTTTACGGTGaaaa                                                   >  1:1690221/1‑67 (MQ=255)
               aGCGCTATTACCGCCAGCCATGGTGATATTGTCATCGCTGCTGTTGACGGCGAGTTTACGGTGaaaa                                                   >  1:376077/1‑67 (MQ=255)
                     aTTACCGCCAGCCATGGTGATATTGTCATCGCTGCTGTTGACGGCGAGTTTACGGTGAAAAAATTGCa                                            >  1:1128669/1‑68 (MQ=255)
                     aTTACCGCCAGCCATGGTGATATTGTCATCGCTGCTGTTGACGGCGAGTTTACGGTGAAAAAATTGCa                                            >  1:2061008/1‑68 (MQ=255)
                        aCCGCCAGCCATGGTGATATTGTCATCGCTGCTGTTGACGGCGAGTTTACGGTGAAAAAATTGCAACt                                         >  1:2614328/1‑68 (MQ=255)
                        aCCGCCAGCCATGGTGATATTGTCATCGCTGCTGTTGACGGCGAGTTTACGGTGAAAAAATTGCAACTa                                        >  1:914426/1‑69 (MQ=255)
                        aCCGCCAGCCATGGTGATATTGTCATCGCTGCTGTTGACGGCGAGTTTACGGTGAAAAAATTGCAACTa                                        >  1:2651528/1‑69 (MQ=255)
                        aCCGCCAGCCATGGTGATATTGTCATCGCTGCTGTTGACGGCGAGTTTACGGTGAAAAAATTGCAACTa                                        >  1:251195/1‑69 (MQ=255)
                        aCCGCCAGCCATGGTGATATTGTCATCGCTGCTGTTGACGGCGAGTTTACGGTGAAAAAATTGCAACTa                                        >  1:1978876/1‑69 (MQ=255)
                          cGCCAGCCATGGTGATATTGTCATCGCTGCTGTTGACGGTGAGTTTACgg                                                         >  1:1030024/1‑50 (MQ=255)
                          cGCCAGCCATGGTGATATTGTCATCGCTGCTGTTGACGGTGAGTTTACGGTGAAAAAATTGCAACTa                                        >  1:1819438/1‑67 (MQ=255)
                          cGCCAGCCATGGTGATATTGTCATCGCTGCTGTTGACGGTGAGTTTACGGTGAAAAAATTGCAACTACg                                      >  1:1101095/1‑69 (MQ=255)
                          cGCCAGCCATGGTGATATTGTCATCGCTGCTGTTGACGGTGAGTTTACGGTGAAAAAATTGCAACTACg                                      >  1:1802668/1‑69 (MQ=255)
                          cGCCAGCCATGGTGATATTGTCATCGCTGCTGTTGACGGTGAGTTTACGGTGAAAAAATTGCAACTACg                                      >  1:2637132/1‑69 (MQ=255)
                          cGCCAGCCATGGTGATATTGTCATCGCTGCTGTTGACGGTGAGTTTACGGTGAAAAAATTGCAACTACg                                      >  1:768725/1‑69 (MQ=255)
                          cGCCAGCCATGGTGATATTGTCATCGCTGCTGTTGACGGTGAGTTTACGGTGAAAAAATTGCAACTACg                                      >  1:2684585/1‑69 (MQ=255)
                          cGCCAGCCATGGTGATATTGTCATCGCTGCTGTTGACGGTGAGTTTACGGTGAAAAAATTGCAACTACg                                      >  1:1586759/1‑69 (MQ=255)
                          cGCCAGCCATGGTGATATTGTCATCGCTGCTGTTGACGGTGAGTTTACGGTGAAAAAATTGCAACTACg                                      >  1:1196811/1‑69 (MQ=255)
                          cGCCAGCCATGGTGATATTGTCATCGCTGCTGTTGACGGTGAGTTTACGGTGAAAAAATTGCAACTACg                                      >  1:2816955/1‑69 (MQ=255)
                          cGCCAGCCATGGTGATATTGTCATCGCTGCTGTTGACGGTGAGTTTACGGTGAAAAAATTGCAACTACg                                      >  1:2246841/1‑69 (MQ=255)
                          cGCCAGCCATGGTGATATTGTCATCGCTGCTGTTGACGGTGAGTTTACGGTGAAAAAATTGCAACTACg                                      >  1:2242786/1‑69 (MQ=255)
                          cGCCAGCCATGGTGATATTGTCATCGCTGCTGTTGACGGTGAGTTTACGGTGAAAAAATTGCAACTACg                                      >  1:2140607/1‑69 (MQ=255)
                          cGCCAGCCATGGTGATATTGTCATCGCTGCTGTTGACGGTGAGTTTACGGTGAAAAAATTGCAACTACg                                      >  1:1823162/1‑69 (MQ=255)
                          cGCCAGCCATGGTGATATTGTCATCGCTGCTGTTGACGGTGAGTTTACGGTGAAAAAATTGCAACTACg                                      >  1:457810/1‑69 (MQ=255)
                          cGCCAGCCATGGTGATATTGTCATCGCTGCTGTTGACGGTGAGTTTACGGTGAAAAAATTGCAACTACg                                      >  1:1954413/1‑69 (MQ=255)
                          cGCCAGCCATGGTGATATTGTCATCGCTGCTGTTGACGGTGAGTTTACGGTGAAAAAATTGCAACTACg                                      >  1:1971645/1‑69 (MQ=255)
                          cGCCAGCCATGGTGATATTGTCATCGCTGCTGTTGACGGTGAGTTTACGGTGAAAAAATTGCAACTACg                                      >  1:1265192/1‑69 (MQ=255)
                          cGCCAGCCATGGTGATATTGTCATCGCTGCTGTTGACGGTGAGTTTACGGTGAAAAAATAGCAACTACg                                      >  1:1226056/1‑69 (MQ=255)
                          cGCCAGCCATGGTGAT‑TTGTCATCGCTGCTGTTGACGGTGAGTTTACGGTGAAAAAATTGCAACTACGc                                     >  1:2107157/1‑69 (MQ=255)
                                ccaTGGTGATATTGTCATCGCTGCTTTTGACGGTGAGTTTACg                                                          <  1:2091119/43‑1 (MQ=38)
                                    ggTGATATTGTCATCGCTGCTGTTGACGGCGAGTTTACGGTGAAAAAATTGCAACTACGCCCGACgg                              >  1:1020767/1‑67 (MQ=255)
                                     gTGATATTGTCATCGCTGCTGTTGACGGCGAGTTTACGGTGAAAAAATTGCAACTACGCCGGACGGt                             >  1:2887942/1‑67 (MQ=255)
                                     gTGATATTGTCATCGCTGCTGTTGACGGCGAGTTTACGGTGAAAAAATTGCAACTACGCCCGACGGt                             >  1:2977915/1‑67 (MQ=255)
                                     gTGATATTGTCATCGCTGCTGTTGACGGCGAGTTTACGGTGAAAAAATTGCAACTACGCCCGACGGt                             >  1:491939/1‑67 (MQ=255)
                                     gTGATATTGTCATCGCTGCTGTTGACGGCGAGTTTACGGTGAAAAAATTGCAACTACGCCCGACGGt                             >  1:2444709/1‑67 (MQ=255)
                                     gTGATATTGTCATCGCTGCTGTTGACGGCGAGTTTACGGTGAAAAAATTGCAACTACGCCCGACGGt                             >  1:1496599/1‑67 (MQ=255)
                                     gTGATATTGTCATCGCTGCTGTTGACGGCGAGTTTACGGTGAAAAAATTGCAACTACGCCCGACGGt                             >  1:1648416/1‑67 (MQ=255)
                                     gTGATATTGTCATCGCTGCTGTTGACGGCGAGTTTACGGTGAAAAAATTGCAACTACGCCCGACGGt                             >  1:796995/1‑67 (MQ=255)
                                     gTGATATTGTCATCGCTGCTGTTGACGGCGAGTTTACGGTGAAAAAATTGCAACTACGCCCGACGGt                             >  1:458464/1‑67 (MQ=255)
                                     gTGATATTGTCATCGCTGCTGTTGACGGCGAGTTTACGGTGAAAAAATTGCAACTACGCCCGACGGt                             >  1:335933/1‑67 (MQ=255)
                                     gTGATATTGTCATCGCTGCTGTTGACGGCGAGTTTACGGTGAAAAAATTGCAACTACGCCCGACGGTa                            >  1:2015510/1‑68 (MQ=255)
                                     gTGATATTGTCATCGCTGCTGTTGACGGCGAGTTTACGGTGAAAAAATTGCAACTACGCCCGACGGTAc                           >  1:2744391/1‑69 (MQ=255)
                                     gTGATATTGTCATCGCTGCTGTTGACGGCGAGTTTACGGTGAAAAAATTGCAACTACGCCCGACGGTAc                           >  1:1898365/1‑69 (MQ=255)
                                            tGTCATCGCTGCTGTTGACGGCGTGTTTACGGTGAAAAAATTGCAACTACGCCCGACGGt                             <  1:968540/60‑1 (MQ=255)
                                            tGTCATCGCTGCTGTTGACGGCGAGTTTAGGGTGAAAAAATTGCAACTACGCCCGACGGt                             <  1:3368257/60‑1 (MQ=255)
                                             gTCATCGCTGCTGTTGACGGCGAGTTTACGGTGAAAAAATTGCAACTACGCCCGACGGTACAGCTTAtt                   >  1:51732/1‑69 (MQ=255)
                                             gTCATCGCTGCTGTTGACGGCGAGTTTACGGTGAAAAAATTGCAACTACGCCCGACGGTACAGCTTAtt                   >  1:1261939/1‑69 (MQ=255)
                                             gTCATCGCTGCTGTTGACGGCGAGTTTACGGTGAAAAAATTGCAACTACGCCCGACGGTACAGCTTAtt                   >  1:2225894/1‑69 (MQ=255)
                                             gTCATCGCTGCTGTTGACGGCGAGTTTACGGTGAAAAAATTGCAACTACGCCCGACGGTACAGCTTAtt                   >  1:2967067/1‑69 (MQ=255)
                                             gTCATCGCTGCTGTTGACGGCGAGTTTACGGTGAAAAAATTGCAACTACGCCCGACGGTACAGCTTAtt                   >  1:147507/1‑69 (MQ=255)
                                             gTCATCGCTGCTGTTGACCGCGAGTTTACCGTGAAAAAATTGc                                             >  1:1678957/1‑43 (MQ=38)
                                                  cGCTGCTGTTGACGGCGAGTTTACGGTGAAAAAATTGCAACTACg                                      >  1:528991/1‑45 (MQ=255)
                                                  cGCTGCTGTTGACGGCGAGTTTACGGTGAAAAAATTGCAACTACGCCCGACGGTACAGCTTATTccc                >  1:1080002/1‑67 (MQ=255)
                                                  cGCTGCTGTTGACGGCGAGTTTACGGTGAAAAAATTGCAACTACGCCCGACGGTACAGCTTATTCCCa               >  1:3154183/1‑68 (MQ=255)
                                                  cGCTGCTGTTGACGGCGAGTTTACGGTGAAAAAATTGCAACTACGCCCGACGGTACAGCTTATTCCCa               >  1:446071/1‑68 (MQ=255)
                                                  cGCTGCTGTTGACGGCGAGTTTACGGTGAAAAAATTGCAACTACGCCCGACGGTACAGCTTATTCCCa               >  1:647699/1‑68 (MQ=255)
                                                  cGCTGCTGTTGACGGCGAGTTTACGGTGAAAAAATTGCAACTACGCCCGACGGTACAGCTTATTCCCa               >  1:3303389/1‑68 (MQ=255)
                                                  cGCTGCTGTTGACGGCGAGTTTACGGTGAAAAAATTGCAACTACGCCCGACGGTACAGCTTATTCCCa               >  1:2266191/1‑68 (MQ=255)
                                                  cGCTGCTGTTGACGGCGAGTTTACGGTGAAAAAATTGCAACTACGCCCGACGGTACAGCTTATTCCCa               >  1:940232/1‑68 (MQ=255)
                                                  cGCTGCTGTTGACGGCGAGTTTACGGTGAAAAAATTGCAACTACGCCCGACGGTACAGCTTATTCCCa               >  1:1391602/1‑68 (MQ=255)
                                                   gctgctGTTGACGGTGAGTTTACGGTGAAAAAATTGCAACTACGCCCGa                                 <  1:1317172/49‑1 (MQ=255)
                                                   gctgctGTTGACGGTGAGTTTACGGTGAAAAAATTGCAACTACGCCCGa                                 <  1:1953415/49‑1 (MQ=255)
                                                   gctgctGTTGACGGTGAGTTTACGGTGAAAAAATTGCAACTACGCCCGa                                 <  1:1444750/49‑1 (MQ=255)
                                                   gctgctGTTGACGGTGAGTTTACGGTGAAAAAATTGCAACTACGCCCGa                                 <  1:1438148/49‑1 (MQ=255)
                                                   gctgctGTTGACGGTGAGTTTACGGTGAAAAAATTGCAACTACGCCCGa                                 <  1:1318019/49‑1 (MQ=255)
                                                   gctgctGTTGACGGTGAGTTTACGGTGAAAAAATTGCAACTACGCCCGa                                 <  1:1760222/49‑1 (MQ=255)
                                                   gctgctGTTGACGGTGAGTTTACGGTGAAAAAATTGCAACTACGCCCGa                                 <  1:1927196/49‑1 (MQ=255)
                                                   gctgctGTTGACGGTGAGTTTACGGTGAAAAAATTGCAACTACGCCCGa                                 <  1:2550342/49‑1 (MQ=255)
                                                   gctgctGTTGACGGTGAGTTTACGGTGAAAAAATTGCAACTACGCCCGa                                 <  1:1031745/49‑1 (MQ=255)
                                                   gctgctGTTGACGGTGAGTTTACGGTGAAAAAATTGCAACTACGCCCGa                                 <  1:2075069/49‑1 (MQ=255)
                                                   gctgctGTTGACGGTGAGTTTACGGTGAAAAAATTGCAACTACGCCCGa                                 <  1:20997/49‑1 (MQ=255)
                                                   gctgctGTTGACGGTGAGTTTACGGTGAAAAAATTGCAACTACGCCCGa                                 <  1:1052884/49‑1 (MQ=255)
                                                   gctgctGTTGACGGTGAGTTTACGGTGAAAAAATTGCAACTACGCCCGa                                 <  1:3038074/49‑1 (MQ=255)
                                                   gctgctGTTGACGGTGAGTTTACGGTGAAAAAATTGCAACTACGCCCGa                                 <  1:1214669/49‑1 (MQ=255)
                                                   gctgctGTTGACGGTGAGTTTACGGTGAAAAAATTGCAACTACGCCCGa                                 <  1:1194095/49‑1 (MQ=255)
                                                   gctgctGTTGACGGTGAGTTTACGGTGAAAAAATTGCAACTACGCCCGa                                 <  1:2714741/49‑1 (MQ=255)
                                                                 cGAGTTTACGGTGAAAAAATTGCAACTACGCCCGACGGTACAGCTTATTCCCATGAACAGCGCGTa  <  1:578929/66‑1 (MQ=255)
                                                                 |                                                                 
TTACTGATTGTCGATAGCGCTATTACCGCCAGCCATGGTGATATTGTCATCGCTGCTGTTGACGGCGAGTTTACGGTGAAAAAATTGCAACTACGCCCGACGGTACAGCTTATTCCCATGAACAGCGCGTA  >  minE/783191‑783321

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A