Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F1 I0 R1
|
4577 |
57.4 |
3436498 |
77.7% |
2670158 |
63.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
800,666 |
C→T |
36.9% |
G221G (GGG→GGA) |
dhaH ← |
fused predicted dihydroxyacetone‑specific PTS enzyme HPr component and EI component |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 800,666 | 0 | C | T | 36.9%
| 51.1
/ 47.9
| 81 | G221G (GGG→GGA) | dhaH | fused predicted dihydroxyacetone‑specific PTS enzyme HPr component and EI component |
| Reads supporting (aligned to +/- strand): ref base C (26/25); new base T (14/16); total (40/41) |
| Fisher's exact test for biased strand distribution p-value = 8.19e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.06e-01 |
TCCTCCGTTTCACCAAAGTTATCTTCAGCCAGCTGACGGAAAGCGATCAGTGCCTCTTCAGCTTCTGGCCCTTTCGCAATCAGGCGCAGCGTATCGTTATAGCGAACTTGTAGTAACGCAATCTGGTTAATA > minE/800598‑800729
|
tcctccGTTTCACCAAAGTTATCTTCAGCCAGCTGACGGAAAGCGATCAGTGCCTCTTCAGCTTCTGGc < 1:1488058/69‑1 (MQ=255)
ttCACCAAAGTTATCTTCAGCCAGCTGACGGAAAGCGATCAGTGCCTCTTCAGCTTCTGGCCCTTTCGc < 1:2748415/69‑1 (MQ=255)
aCCAAAGTTATCTTCAGCCAGCTGACGGAAAGCGATCAGTGCCTCTTCAGCTTCTGGCCCTTTCGCaa < 1:1180429/68‑1 (MQ=255)
ttCAGCCAGCTGACGGAAAGCGATCAGTGCCTCTTCAGCTTCTGGTCCTTTCGCAATCAGGCGCAGcc < 1:2863186/68‑2 (MQ=255)
ttCAGCCAGCTGACGGAAAGCGATCAGTGCCTCTTCAGCTTCTGGTCCTTTCGCAATCAGGCGCAGCg < 1:20176/68‑1 (MQ=255)
ttCAGCCAGCTGACGGAAAGCGATCAGTGCCTCTTCAGCTTCTGGTCCTTTCGCAATCAGGCGCAGCg < 1:2030303/68‑1 (MQ=255)
ttCAGCCAGCTGACGGAAAGCGATCAGTGCCTCTTCAGCTTCTGGTCCTTTCGCAATCAGGCGCAGCg < 1:2049702/68‑1 (MQ=255)
ttCAGCCAGCTGACGGAAAGCGATCAGTGCCTCTTCAGCTTCTGGTCCTTTCGCAATCAGGCGCAGCg < 1:172245/68‑1 (MQ=255)
ttCAGCCAGCTGACGGAAAGCGATCAGTGCCTCTTCAGCTTCTGGTCCTTTCGCAATCAGGCGCAGCg < 1:2377722/68‑1 (MQ=255)
ttCAGCCAGCTGACGGAAAGCGATCAGTGCCTCTTCAGCTTCTGGTCCTTTCGCAATCAGGCGCAGCg < 1:2443062/68‑1 (MQ=255)
ttCAGCCAGCTGACGGAAAGCGATCAGTGCCTCTTCAGCTTCTGGTCCTTTCGCAATCAGGCGCAGCg < 1:2592635/68‑1 (MQ=255)
ttCAGCCAGCTGACGGAAAGCGATCAGTGCCTCTTCAGCTTCTGGTCCTTTCGCAATCAGGCGCAGCg < 1:2646498/68‑1 (MQ=255)
ttCAGCCAGCTGACGGAAAGCGATCAGTGCCTCTTCAGCTTCTGGTCCTTTCGCAATCAGGCGCAGCg < 1:1345051/68‑1 (MQ=255)
ttCAGCCAGCTGACGGAAAGCGATCAGTGCCTCTTCAGCTTCTGGTCCTTTCGCAATCAGGCGCAGCg < 1:1337067/68‑1 (MQ=255)
ttCAGCCAGCTGACGGAAAGCGATCAGTGCCTCTTCAGCTTCTGGTCCTTTCGCAATCAGGCGCAGCg < 1:1331440/68‑1 (MQ=255)
ttCAGCCAGCTGACGGAAAGCGATCAGTGCCTCTTCAGCTTCTGGTCCTTTCGCAATCAGGCGCAGCg < 1:2950304/68‑1 (MQ=255)
ttCAGCCAGCTGACGGAAAGCGATCAGTGCCTCTTCAGCTTCTGGTCCTTTCGCAATCAGGCGCAGCg < 1:3303494/68‑1 (MQ=255)
ttCAGCCAGCTGACGGAAAGCGATCAGTGCCTCTTCAGCTTCTGGTCCTTTCGCAATCAGGCGCAGCg < 1:690354/68‑1 (MQ=255)
tCAGCCAGCTGACGGAAAGCGATCAGTGCCTCTTCAGCTTCTGGCCCTTTCGCAATCAGGCGCAGCGt < 1:1302530/68‑1 (MQ=255)
gccagcTGACGGAAAGCGATCAGTGCTTCTTCAGCTTCTGGCCCTTTCGCAATCAGGCGCAGCGTAt < 1:3131887/67‑1 (MQ=255)
gccagcTGACGGAAAGCGATCAGTGCCTCTTCAGCTTCTGGCCCTTTCGCAATCAGGCGCAGCGt < 1:657805/65‑1 (MQ=255)
gccagcTGACGGAAAGCGATCAGTGCCTCTTCAGCTTCTGGCCCTTTCGCAATCAGGCGCAGCGt < 1:216088/65‑1 (MQ=255)
gccagcTGACGGAAAGCGATCAGTGCCTCTTCAGCTTCTGGCCCTTTCGCAATCAGGCGCAGCGt < 1:796020/65‑1 (MQ=255)
gccagcTGACGGAAAGCGATCAGTGCCTCTTCAGCTTCTGGCCCTTTCGCAATCAGGCGCAGCGt < 1:992531/65‑1 (MQ=255)
gccagcTGACGGAAAGCGATCAGTGCCTCTTCAGCTTCTGGCCCTTTCGCAATCAGGCGCAGCGt < 1:1603421/65‑1 (MQ=255)
ccagcTGACGGAAAGCGATCAGTGCCTCTTCAGCTTCTGGCCCTTTCGCAATCAGGCGCAGCGt < 1:1872696/64‑1 (MQ=255)
ccagcTGACGGAAAGCGATCAGTGCCTCTTCAGCTTCTGGCCCTTTCGCAATCAGGCGCAGCGTATCGt < 1:1684866/69‑1 (MQ=255)
ccagcTGACGGAAAGCGATCAGTGCATCTTCAGCTTCTGGCCCTTTCGCAATCAGGCGCAGCGTATCGt < 1:2906203/69‑1 (MQ=255)
aGCGATCAGTGCCTCTTCAGCTTCTGGCCCTTTCGCAATCAGGCGCAGCGTATCGTTATAGCGAACTTg < 1:3205199/69‑1 (MQ=255)
aGCGATCAGTGCATCTTCAGCTTCTGGCCCTTTCGCAATCAGGCGCAGCGTATCGTTATAGCGAACTTg < 1:951448/69‑1 (MQ=255)
gCGATCAGTGCCTCTTCAGCTTCTGGCCCTTTCGCAATCAGGCGCAGCGTATCGTTATAGCGAACTTg < 1:2562522/68‑1 (MQ=255)
aTCAGTGCCTCTTCAGCTTCTGGTCCTTTCGCAATCAGGCGCAGCg < 1:3041843/46‑1 (MQ=255)
cAGTGCCTCTTCAGCTTCTGGCCCTTTCGCAATCAGGCGCAGCGTATCGTTATAGCg < 1:242628/57‑1 (MQ=255)
aGTGCCTCTTTAGCTTCTGGCCCTTTCGCAATCAGGCGCAGCGTATCGTTATAGCGAACTTgtagt > 1:3004697/1‑66 (MQ=255)
aGTGCCTCTTCAGCTTCTGGCCCTTTCGCAATCAGGCGCAGCGTATCGTTATAGCGAACTTgtagt > 1:2732034/1‑66 (MQ=255)
aGTGCCTCTTCAGCTTCTGGCCCTTTCGCAATCAGGCGCAGCGTATCGTTATAGCGAACTTgtagt > 1:27070/1‑66 (MQ=255)
aGTGCCTCTTCAGCTTCTGGCCCTTTCGCAATCAGGCGCAGCGTATCGTTATAGCGAACTTgtagt > 1:2865226/1‑66 (MQ=255)
aGTGCCTCTTCAGCTTCTGGCCCTTTCGCAATCAGGCGCAGCGTATCGTTATAGCGAACTTgtagt > 1:1261778/1‑66 (MQ=255)
aGTGCCTCTTCAGCTTCTGGCCCTTTCGCAATCAGGCGCAGCGTATCGTTATAGCGAACTTgtagt > 1:2967217/1‑66 (MQ=255)
aGTGCCTCTTCAGCTTCTGGCCCTTTCGCAATCAGGCGCAGCGTATCGTTATAGCGAACTTgtagt > 1:2994995/1‑66 (MQ=255)
aGTGCCTCTTCAGCTTCTGGCCCTTTCGCAATCAGGCGCAGCGTATCGTTATAGCGAACTTgtagt > 1:3087548/1‑66 (MQ=255)
aGTGCCTCTTCAGCTTCTGGCCCTTTCGCAATCAGGCGCAGCGTATCGTTATAGCGAACTTgtagt > 1:2202601/1‑66 (MQ=255)
aGTGCCTCTTCAGCTTCTGGCCCTTTCGCAATCAGGCGCAGCGTATCGTTATAGCGAACTTgtagt > 1:3418887/1‑66 (MQ=255)
gCCTCTTCAGCTTCTGGCCCTTTCGCAATCAGGCGCAGCGTATCGTTATAGCGAACTTGTAGTAACGCa < 1:2988502/69‑1 (MQ=255)
gCCTCTTCAGCTTCTGGCCCTTTCGCAATCAGGCGCAGCGTATCGTTATAGCGAACTTGTAGTAACGCa < 1:2266185/69‑1 (MQ=255)
gCCTCTTCAGCTTCTGGCCCTTTCGCAATCAGGCGCAGCGTATCGTTATAGCGAACTTGTAGTAACGCa < 1:2882896/69‑1 (MQ=255)
ccTCTTCAGCTTCTGGCCCTTTCGCAATCAGGCGCAGCGTATCGTTATAGCGAACTTGTAGTAACGCaa > 1:3156922/1‑69 (MQ=255)
ccTCTTCAGCTTCTGGCCCTTTCGCAATCAGGCGCAGCGTATCGTTATAGCGAACTTGTAGTAACGCaa > 1:3387166/1‑69 (MQ=255)
ccTCTTCAGCTTCTGGCCCTTTCGCAATCAGGCGCAGCGTATCGTTATAGCGAACTTGTAGTAACGCaa > 1:1494252/1‑69 (MQ=255)
ccTCTTCAGCTTCTGGCCCTTTCGCAATCAGGCGCAGCGTATCGTTATAGCGAACTTGTAGTAACGCaa > 1:2044782/1‑69 (MQ=255)
ccTCTTCAGCTTCTGGCCCTTTCGCAATCAGGCGCAGCGTATCGTTATAGCGAACTTGTAGTAACGCaa > 1:2258429/1‑69 (MQ=255)
cAGCTTCTGGCCCTTTCGCAATCAGGCGCAGCGTATCGTTATAGCGAACTTGTAGTAACGCAATCTgg > 1:2721440/1‑68 (MQ=255)
cAGCTTCTGGCCCTTTCGCAATCAGGCGCAGCGTATCGTTATAGCGAACTTGTAGTAACGCAATCTgg > 1:2724056/1‑68 (MQ=255)
cAGCTTCTGGCCCTTTCGCAATCAGGCGCAGCGTATCGTTATAGCGAACTTGTAGTAACGCAATCTgg > 1:2626703/1‑68 (MQ=255)
cAGCTTCTGGCCCTTTCGCAATCAGGCGCAGCGTATCGTTATAGCGAACTTGTAGTAACGCAATCTgg > 1:2097217/1‑68 (MQ=255)
cAGCTTCTGGCCCTTTCGCAATCAGGCGCAGCGTATCGTTATAGCGAACTTGTAGTAACGCAATCTgg > 1:1134057/1‑68 (MQ=255)
cAGCTTCTGGCCCTTTCGCAATCAGGCGCAGCGTATCGTTATAGCGAACTTGTAGTAACGCAATCTgg > 1:1123125/1‑68 (MQ=255)
cAGCTTCTGGCCCTTTCGCAATCAGGCGCAGCGTATCGTTATAGCGAACTTGTAGTAACGCAATCTgg > 1:2369143/1‑68 (MQ=255)
cAGCTTCTGGCCCTTTCGCAATCAGGCGCAGCGTATCGTTATAGCGAACTTGTAGTAACGCAATCTg > 1:2363585/1‑67 (MQ=255)
cAGCTTCTGGCCCTTTCGCAATCAGGCGCAGCGTATCGTTATAGCGAACTTGTAGTAACGCAATCTg > 1:3423869/1‑67 (MQ=255)
cAGCTTCTGGCCCTTTCGCAATCAGGCGCAGCGTATCGTTATAGCGAACTTGTAGTAACGCAATCTg > 1:2076108/1‑67 (MQ=255)
cAGCTTCTGGCCCTTTCGCAATCAGGCGCAGCGTATCGTTATAGCGAACTTGTAGTAACGCAATCTg > 1:1393026/1‑67 (MQ=255)
gCTTCTGGCCCTTTCGCAATCAGGCGCAGCGTATCGTTATAGCGAACTTGTAGTAACGCAATCTGGtt < 1:2131304/68‑1 (MQ=255)
gCTTCTGGCCCTTTCGCAATCAGGCGCAGCGTATCGTTATAGCGAACTTGTAGTAACGCAATCTGGtt < 1:3343861/68‑1 (MQ=255)
gCTTCTGGCCCTTTCGCAATCAGGCGCAGCGTATCGTTATAGCGAACTTGTAGTAACGCAATCTGGt < 1:115688/67‑1 (MQ=255)
gCTTCTGGCCCTTTCGCAATCAGGCGCAGCGTATCGTTATAGCGAACTTGTAGTAACGCAATCTGGt < 1:1778835/67‑1 (MQ=255)
gCTTCTGGCCCTTTCGCAATCAGGCGCAGCGTATCGTTATAGCGAACTTGTAGTAACGCAATCTGGt < 1:2643515/67‑1 (MQ=255)
tCTGGTCCTTTCGCAATCAGGCGCAGCGTATCGTTATAGCGAACTTg > 1:1057596/1‑47 (MQ=255)
tCTGGTCCTTTCGCAATCAGGCGCAGCGTATCGTTATAGCGAACTTg > 1:762522/1‑47 (MQ=255)
tCTGGTCCTTTCGCAATCAGGCGCAGCGTATCGTTATAGCGAACTTg > 1:1107719/1‑47 (MQ=255)
tCTGGTCCTTTCGCAATCAGGCGCAGCGTATCGTTATAGCGAACTTg > 1:1091136/1‑47 (MQ=255)
tCTGGTCCTTTCGCAATCAGGCGCAGCGTATCGTTATAGCGAACTTg > 1:973072/1‑47 (MQ=255)
tCTGGTCCTTTCGCAATCAGGCGCAGCGTATCGTTATAGCGAACTTg > 1:621116/1‑47 (MQ=255)
tCTGGTCCTTTCGCAATCAGGCGCAGCGTATCGTTATAGCGAACTTg > 1:392418/1‑47 (MQ=255)
tCTGGTCCTTTCGCAATCAGGCGCAGCGTATCGTTATAGCGAACTTg > 1:3247342/1‑47 (MQ=255)
tCTGGTCCTTTCGCAATCAGGCGCAGCGTATCGTTATAGCGAACTTg > 1:1766277/1‑47 (MQ=255)
tCTGGTCCTTTCGCAATCAGGCGCAGCGTATCGTTATAGCGAACTTg > 1:2387674/1‑47 (MQ=255)
tCTGGTCCTTTCGCAATCAGGCGCAGCGTATCGTTATAGCGAACTTg > 1:2424795/1‑47 (MQ=255)
tCTGGTCCTTTCGCAATCAGGCGCAGCGTATCGTTATAGCGAACTTg > 1:2433031/1‑47 (MQ=255)
tCTGGTCCTTTCGCAATCAGGCGCAGCGTATCGTTATAGCGAACTTg > 1:1567584/1‑47 (MQ=255)
tCTGGTCCTTTCGCAATCAGGCGCAGCGTATCGTTATAGCGAACTTg > 1:1559646/1‑47 (MQ=255)
tCTGGCCCTTTCGCAATCAGGCGCAGCGTATCGTTATAGCGAACTTGTAGTAACGCAATCTGGTTAATa < 1:428910/69‑1 (MQ=255)
|
TCCTCCGTTTCACCAAAGTTATCTTCAGCCAGCTGACGGAAAGCGATCAGTGCCTCTTCAGCTTCTGGCCCTTTCGCAATCAGGCGCAGCGTATCGTTATAGCGAACTTGTAGTAACGCAATCTGGTTAATA > minE/800598‑800729
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
