Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F1 I0 R1
|
4577 |
57.4 |
3436498 |
77.7% |
2670158 |
63.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
801,843 |
G→A |
32.0% |
N43N (AAC→AAT) |
dhaL ← |
dihydroxyacetone kinase, C‑terminal domain |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 801,843 | 0 | G | A | 32.0%
| 72.4
/ 38.8
| 81 | N43N (AAC→AAT) | dhaL | dihydroxyacetone kinase, C‑terminal domain |
| Reads supporting (aligned to +/- strand): ref base G (16/39); new base A (9/17); total (25/56) |
| Fisher's exact test for biased strand distribution p-value = 6.17e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
GAAACCGATATCTTTATCTGCGATAGCAGGGAGTTTTTCCACCACTTTGCTAAAGCCTCGGTTCATATTTAGCCCGTGGTCAGCATCGCCAATTTCGCGATCCAGTCCGGTAAGA > minE/801783‑801897
|
gAAACCGATATCTTTATCTGCGATAGCAGGGAGTTTTTCCACCACTTTGCTAAAGCCTCGGTTCata > 1:1044270/1‑67 (MQ=255)
gAAACCGATATCTTTATCTGCGATAGCAGGGAGTTTTTCCACCACTTTGCTAAAGCCTCGGTTCata > 1:692258/1‑67 (MQ=255)
aaaCCGATATCTTTATCTGCGATAGCAGGGAGTTTTTCCACCACTTTGCTAAAGCCTCGGTTCATAttt < 1:3055151/69‑1 (MQ=255)
aaaCCGATATCTTTATCTGCGATAGCAGGGAGTTTTTCCACCACTTTGCTAAAGCCTCGGTTCATAttt < 1:496528/69‑1 (MQ=255)
aaaCCGATATCTTTATCTGCGATAGCAGGGAGTTTTTCCACCACTTTGCTAAAGCCTCGGTTCATAttt < 1:2246450/69‑1 (MQ=255)
aaaCCGATATCTTTATCTGCGATAGCAGGGAGTTTTTCCACCACTTTGCTAAAGCCTCGGTTCATAttt < 1:189799/69‑1 (MQ=255)
aaaCCGATATCTTTATCTGCGATAGCAGGGAGTTTTTCCACCACTTTGCTAAAGCCTCGGTTCATAttt < 1:33377/69‑1 (MQ=255)
aaaCCGATATCTTTATCTGCGATAGCAGGGAGTTTTTCCACCACTTTGCTAAAGCCTCGGTTCATAttt > 1:3282847/1‑69 (MQ=255)
aaaCCGATATCTTTATCTGCGATAGCAGGGAGTTTTTCCACCACTTTGCTAAAGCCTCGGTTCATAttt < 1:1607565/69‑1 (MQ=255)
aaaCCGATATCTTTATCTGCGATAGCAGGGAGTTTTTCCACCACTTTGCTAAAGCCTCGGTTCATAttt > 1:3225136/1‑69 (MQ=255)
aaaCCGATATCTTTATCTGCGATAGCAGGGAGTTTTTCCACCACTTTGCTAAAGCCTCGGTTCATAttt < 1:155148/69‑1 (MQ=255)
aaaCCGATATCTTTATCTGCGATAGCAGGGAGTTTTTCCACCACTTTGCTAAAGCCTCGGTTCATAttt > 1:1495967/1‑69 (MQ=255)
aaaCCGATATCTTTATCTGCGATAGCAGGGAGTTTTTCCACCACTTTGCTAAAGCCTCGGTTCATAttt < 1:1349768/69‑1 (MQ=255)
aaaCCGATATCTTTATCTGCGATAGCAGGGAGTTTTTCCACCACTTTGCTAAAGCCTCGGTTCATAttt > 1:1335837/1‑69 (MQ=255)
aaaCCGATATCTTTATCTGCGATAGCAGGGAGTTTTTCCACCACTTTGCTAAAGCCTCGGTTCATAttt < 1:810471/69‑1 (MQ=255)
aaaCCGATATCTTTATCTGCGATAGCAGGGAGTTTTTCCACCACTTTGCTAAAGCCTCGGTTCATAttt > 1:3189269/1‑69 (MQ=255)
aaaCCGATATCTTTATCTGCGATAGCAGGGAGTTTTTCCACCACTTTGCTAAAGCCTCGGTTCATAttt < 1:2871361/69‑1 (MQ=255)
aaaCCGATATCTTTATCTGCGATAGCAGGGAGTTTTTCCACCACTTTGCTAAAGCCTCGGTTCATAttt < 1:1240578/69‑1 (MQ=255)
aaaCCGATATCTTTATCTGCGATAGCAGGGAGTTTTTCCACCACTTTGCTAAAGCCTCGGTTCATAttt < 1:3058413/69‑1 (MQ=255)
aaaCCGATATCTTTATCTGCGATAGCAGGGAGTTTTTCCACCACTTTGCTAAAGCCTCGGTTCATAttt > 1:1118008/1‑69 (MQ=255)
aaaCCGATATCTTTATCTGCGATAGCAGGGAGTTTTTCCACCACTTTGCTAAAGCCTCGGTTCATAttt < 1:296916/69‑1 (MQ=255)
atCTTTATCTGCGATAGCAGGGAGTTTTTCCACCACTTTGCTAAAGCCTCGGTTCATATTTAGCCCGTg < 1:75613/69‑1 (MQ=255)
tCTTTATCTGCGATAGCAGGGAGTTTTTCCACCACTTTGCTAAAGCCTCGGTTCATATTTAGCCCGTg < 1:3108239/68‑1 (MQ=255)
cTTTATCTGCGATAGCAGGGAGTTTTTCCACCACTTTGCTAAAGCCTCGATTCATATTTAGCCCGTg > 1:2756380/1‑67 (MQ=255)
cTTTATCTGCGATAGCAGGGAGTTTTTCCACCACTTTGCTAAAGCCTCGATTCATATTTAGCCCGTg > 1:2928973/1‑67 (MQ=255)
cTTTATCTGCGATAGCAGGGAGTTTTTCCACCACTTTGCTAAAGCCTCGATTCATATTTAGCCCGTg > 1:1036781/1‑67 (MQ=255)
cTTTATCTGCGATAGCAGGGAGTTTTTCCACCACTTTGCTAAAGCCTCGATTCATATTTAGCCCGTg > 1:406567/1‑67 (MQ=255)
cTTTATCTGCGATAGCAGGGAGTTTTTCCACCACTTTGCTAAAGCCTCGATTCATATTTAGCCCGTg > 1:426788/1‑67 (MQ=255)
cTTTATCTGCGATAGCAGGGAGTTTTTCCACCACTTTGCTAAAGCCTCGATTCATATTTAGCCCGTg > 1:517286/1‑67 (MQ=255)
cTTTATCTGCGATAGCAGGGAGTTTTTCCACCACTTTGCTAAAGCCTCGATTCATATTTAGCCCGTg > 1:605007/1‑67 (MQ=255)
cTTTATCTGCGATAGCAGGGAGTTTTTCCACCACTTTGCTAAAGCCTCGATTCATATTTAGCCCGTg > 1:1274429/1‑67 (MQ=255)
cTTTATCTGCGATAGCAGGGAGTTTTTCCACAACTTTGCTAAAGCCTCGATTCATATTTAGCCCGTg > 1:656336/1‑67 (MQ=255)
tCTGCGATAGCAGGGAGTTTTTCCACCACTTTGCTAAAGCCTCGGTTCATATTTAGCCCGTGGTCAg < 1:2963315/67‑1 (MQ=255)
tCTGCGATAGCAGGGAGTTTTTCCACCACTTTGCTAAAGCCTCGGTTCATATTTAGCCCGTGGTCAg < 1:3155693/67‑1 (MQ=255)
tCTGCGATAGCAGGGAGTTTTTCCACCACTTTGCTAAAGCCTCGGTTCATATTTAGCCCGTGGTCAGc < 1:2805308/68‑1 (MQ=255)
tCTGCGATAGCAGGGAGTTTTTCCACCACTTTGCTAAAGCCTCGGTTCATATTTAGCCCGTGGTCAGc < 1:1103830/68‑1 (MQ=255)
tCTGCGATAGCAGGGAGTTTTTCCACCACTTTGCTAAAGCCTCGGTTCATATTTAGCCCGTGGTCAGc < 1:114698/68‑1 (MQ=255)
tCTGCGATAGCAGGGAGTTTTTCCACCACTTTGCTAAAGCCTCGGTTCATATTTAGCCCGTGGTCAGc < 1:838937/68‑1 (MQ=255)
tCTGCGATAGCAGGGAGTTTTTCCACCACTTTGCTAAAGCCTCGGTTCATATTTAGCCCGTGGTCAGc < 1:1215110/68‑1 (MQ=255)
tCTGCGATAGCAGGGAGTTTTTCCACCACTTTGCTAAAGCCTCGGTTCATATTTAGCCCGTGGTCAGc < 1:127420/68‑1 (MQ=255)
tCTGCGATAGCAGGGAGTTTTTCCACCACTTTGCTAAAGCCTCGGTTCATATTTAGCCCGTGGTCAGc < 1:2722483/68‑1 (MQ=255)
tCTGCGATAGCAGGGAGTTTTTCCACCACTTTGCTAAAGCCTCGGTTCATATTTAGCCCGTGGTCAGc < 1:591906/68‑1 (MQ=255)
tCTGCGATAGCAGGGAGTTTTTCCACCACTTTGCTAAAGCCTCGGTTCATATTTAGCCCGTGGTCAGc < 1:3291983/68‑1 (MQ=255)
tCTGCGATAGCAGGGAGTTTTTCCACCACTTTGCTAAAGCCTCGGTTCATATTTAGCCCGTGGTCAGc < 1:1940841/68‑1 (MQ=255)
tCTGCGATAGCAGGGAGTTTTTCCACCACTTTGCTAAAGCCTCGGTTCATATTTAGCCCGTGGTCAGc < 1:2178116/68‑1 (MQ=255)
gCAGGGAGTTTTTCCACCACTTTGCTAAAGCCTCGGTTCATATTTAGCCCGTGGTCAGCATCGCCAAtt > 1:3123347/1‑69 (MQ=255)
gCAGGGAGTTTTTCCACCACTTTGCTAAAGCCTCGGTTCATATTTAGCCCGTGGTCAGCATCGCCAAt > 1:2100897/1‑68 (MQ=255)
tttttCCACCACTTTGCTAAAGCCTCGGTTCATATTTAGCCCGTGGTCAGCATCGCCAATTTCGCGATc < 1:2870184/69‑1 (MQ=255)
tttttCCACCACTTTGCTAAAGCCTCGGTTCATATTTAGCCCGTGGTCAGCATCGCCAATTTCGCGATc < 1:1858456/69‑1 (MQ=255)
tttttCCACCACTTTGCTAAAGCCTCGGTTCATATTTAGCCCGTGGTCAGCATCGCCAATTTCGCGATc < 1:1325984/69‑1 (MQ=255)
tttCCACCACTTTGCTAAAGCCTCGGTTCATATTTAGCCCGTGGTCAGCATCGCCAATTTCGCGATCCa > 1:305652/1‑69 (MQ=255)
tttCCACCACTTTGCTAAAGCCTCGGTTCATATTTAGCCCGTGGTCAGCATCGCCAATTTCGCGATCCa > 1:3077090/1‑69 (MQ=255)
ccacTTTGCTAAAGCCTCGGTTCATATTTAGCCCGTGGTCAGCATCGCCAAt > 1:2501877/1‑52 (MQ=255)
ccacTTTGCTAAAGCCTCGGTTCATATTTAGCCCGTGGTCAGCATCGCCAATTTCGCGATCCAGTCCg > 1:1315207/1‑68 (MQ=255)
ccacTTTGCTAAAGCCTCGGTTCATATTTAGCCCGTGGTCAGCATCGCCAATTTCGCGATCCAGTCCg > 1:2911026/1‑68 (MQ=255)
ccacTTTGCTAAAGCCTCGGTTCATATTTAGCCCGTGGTCAGCATCGCCAATTTAGCGATCCAGTcc > 1:2862321/1‑67 (MQ=255)
cacTTTGCTAAAGCCTCGGTTCATATTTAGCCCGTGGTCAGCATCGCCAATTTCGCGATCCAGTCCGGt < 1:1167752/69‑1 (MQ=255)
cacTTTGCTAAAGCCTCGGTTCATATTTAGCCCGTGGTCAGCATCGCCAATTTCGCGATCCAGTCCGGt < 1:1677831/69‑1 (MQ=255)
cTTTGCTAAAGCCTCGGTTCATATTTAGCCCGTGGTCAGCATCGCCAATTTCGCGATCCAGTCCGGTaa < 1:848233/69‑1 (MQ=255)
cTTTGCTAAAGCCTCGATTCATATTTAGCCCGTGGTCAGCGTCGCCAATCTCGCGATccagtccagt < 1:896930/67‑1 (MQ=255)
cTTTGCTAAAGCCTCGATTCATATTTAGCCCGTGGTCAGCGTCGCCAATCTCGCGATccagtccagt < 1:1293883/67‑1 (MQ=255)
cTTTGCTAAAGCCTCGATTCATATTTAGCCCGTGGTCAGCGTCGCCAATCTCGCGATccagtccagt < 1:155932/67‑1 (MQ=255)
cTTTGCTAAAGCCTCGATTCATATTTAGCCCGTGGTCAGCGTCGCCAATCTCGCGATccagtccagt < 1:59896/67‑1 (MQ=255)
cTTTGCTAAAGCCTCGATTCATATTTAGCCCGTGGTCAGCGTCGCCAATCTCGCGATccagtccagt < 1:574073/67‑1 (MQ=255)
cTTTGCTAAAGCCTCGATTCATATTTAGCCCGTGGTCAGCGTCGCCAATCTCGCGATccagtccagt < 1:906946/67‑1 (MQ=255)
cTTTGCTAAAGCCTCGATTCATATTTAGCCCGTGGTCAGCGTCGCCAATCTCGCGATccagtccagt < 1:2077842/67‑1 (MQ=255)
cTTTGCTAAAGCCTCGATTCATATTTAGCCCGTGGTCAGCGTCGCCAATCTCGCGATccagtccagt < 1:2145183/67‑1 (MQ=255)
cTTTGCTAAAGCCTCGATTCATATTTAGCCCGTGGTCAGCGTCGCCAATCTCGCGATccagtccagt < 1:2493110/67‑1 (MQ=255)
cTTTGCTAAAGCCTCGATTCATATTTAGCCCGTGGTCAGCGTCGCCAATCTCGCGATccagtccagt < 1:3244665/67‑1 (MQ=255)
cTTTGCTAAAGCCTCGATTCATATTTAGCCCGTGGTCAGCGTCGCCAATCTCGCGATccagtccagt < 1:2717444/67‑1 (MQ=255)
cTTTGCTAAAGCCTCGATTCATATTTAGCCCGTGGTCAGCGTCGCCAATCTCGCGATccagtccagt < 1:3207741/67‑1 (MQ=255)
cTTTGCTAAAGCCTCGATTCATATTTAGCCCGTGGTCAGCGTCGCCAATCTCGCGATccagtccagt < 1:2828593/67‑1 (MQ=255)
cTTTGCTAAAGCCTCGATTCATATTTAGCCCGTGGTCAGCGTCGCCAATCTCGCGATccagtccagt < 1:3176461/67‑1 (MQ=255)
cTTTGCTAAAGCCTCGATTCATATTTAGCCCGTGGTCAGCGTCGCCAATCTCGCGATccagtccagt < 1:1019241/67‑1 (MQ=255)
cTTTGCTAAAGCCTCGATTCATATTTAGCCCGTGGTCAGCGTCGCCAATCTCGCGATccagtccagt < 1:2904497/67‑1 (MQ=255)
tttGCTAAAGCCTCGATTCATATTTAGCCCGTGGTCAGCGTCGCCAATCTCGCGATCCAGTCCTGt < 1:880809/66‑1 (MQ=255)
ttGCTAAAGCCTCGGTTCATATTTAGCCCGTGGTCAGc < 1:2994650/38‑1 (MQ=255)
ttGCTAAAGCCTCGGTTCATATTTAGCCCGTGGTCAGCATCGCCAATTTCGCGATCCAGTCCGGTAAGa < 1:2043236/69‑1 (MQ=255)
ttGCTAAAGCCTCGGTTCATATTTAGCCCGTGGTCAGCATCGCCAATTTCGCGATCCAGTCCGGTAAGa < 1:2434993/69‑1 (MQ=255)
ttGCTAAAGCCTCGGTTCATATTTAGCCCGTGGTCAGCATCGCCAATTTCGCGATCCAGTCCGGTAAGa < 1:3151014/69‑1 (MQ=255)
cTAAAGCCTCGGTTCATATTTAGCCCGTGGTCAGc < 1:156647/35‑1 (MQ=255)
|
GAAACCGATATCTTTATCTGCGATAGCAGGGAGTTTTTCCACCACTTTGCTAAAGCCTCGGTTCATATTTAGCCCGTGGTCAGCATCGCCAATTTCGCGATCCAGTCCGGTAAGA > minE/801783‑801897
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
