Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F1 I0 R1
|
4577 |
57.4 |
3436498 |
77.7% |
2670158 |
63.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
817,435 |
T→C |
36.9% |
D70D (GAT→GAC) |
prfA → |
peptide chain release factor RF‑1 |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 817,435 | 0 | T | C | 36.9%
| 70.0
/ 75.5
| 119 | D70D (GAT→GAC) | prfA | peptide chain release factor RF‑1 |
| Reads supporting (aligned to +/- strand): ref base T (28/47); new base C (8/36); total (36/83) |
| Fisher's exact test for biased strand distribution p-value = 3.82e-02 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.21e-01 |
Only 100 of 122 total aligned reads displayed.
CTGTTTTACCGACTGGCAACAGGTTCAGGAAGATATCGAAACCGCACAGATGATGCTCGATGATCCTGAAATGCGTGAGATGGCGCAGGATGAACTGCGCGAAGCTAAAGAAAAAAGCGAGCAACTGG > minE/817375‑817502
|
cTGTTTTACCGACTGGCAACAGGTTCAGGAAGATATCGAAACCGCTCAGATGATGCTCGACGATCCTGa < 1:2662384/69‑1 (MQ=255)
cTGTTTTACCGACTGGCAACAGGTTCAGGAAGATATCGAAACCGCACAGATGATGCTCGATGATCCTGa < 1:903987/69‑1 (MQ=255)
cTGTTTTACCGACTGGCAACAGGTTCAGGAAGATATCGAAACCGCACAGATGATGCTCGATGATCCTGa < 1:808893/69‑1 (MQ=255)
cTGTTTTACCGACTGGCAACAGGTTCAGGAAGATATCGAAACCGCACAGATGATGCTCGATGATCCTGa < 1:2153266/69‑1 (MQ=255)
cTGTTTTACCGACTGGCAACAGGTTCAGGAAGATATCGAAACCGCACAGATGATGCTCGATGATCCTGa < 1:2945916/69‑1 (MQ=255)
cTGTTTTACCGACTGGCAACAGGTTCAGGAAGATATCGAAACCGCACAGATGATGCTCGATGATCCTGa < 1:3075161/69‑1 (MQ=255)
cTGTTTTACCGACTGGCAACAGGTTCAGGAAGATATCGAAACCGCACAGATGATGCTCGACGATCCTGa < 1:2137582/69‑1 (MQ=255)
cTGTTTTACCGACTGGCAACAGGTTCAGGAAGATATCGAAACCGCACAGATGATGCTCGACGATCCTGa < 1:2140487/69‑1 (MQ=255)
cTGTTTTACCGACTGGCAACAGGTTCAGGAAGATATCGAAACCGCACAGATGATGCTCGACGATCCTGa < 1:2055166/69‑1 (MQ=255)
cTGTTTTACCGACTGGCAACAGGTTCAGGAAGATATCGAAACCGCACAGATGATGCTCGACGATCCTGa < 1:2578725/69‑1 (MQ=255)
cTGTTTTACCGACTGGCAACAGGTTCAGGAAGATATCGAAACCGCACAGATGATGCTCGACGATCCTGa < 1:263663/69‑1 (MQ=255)
cTGTTTTACCGACTGGCAACAGGTTCAGGAAGATATCGAAACCGCACAGATGATGCTCGACGATCCTGa < 1:1581511/69‑1 (MQ=255)
cTGTTTTACCGACTGGCAACAGGTTCAGGAAGATATCGAAACCGCACAGATGATGCTCGACGATCCTGa < 1:3342929/69‑1 (MQ=255)
cTGTTTTACCGACTGGCAACAGGTTCAGGAAGATATCGAAACCGCACAGATGATGCTCGACGATCCTGa < 1:373790/69‑1 (MQ=255)
cTGTTTTACCGACTGGCAACAGGTTCAGGAAGATATCGAAACCGCACAGATGATGCTCGACGATCCTGa < 1:799851/69‑1 (MQ=255)
cTGTTTTACCGACTGGCAACAGGTTCAGGAAGATATCGAAACCGCACAGATGATGCTCGACGATCCTGa < 1:442705/69‑1 (MQ=255)
cTGTTTTACCGACTGGCAACAGGTTCAGGAAGATATCGAAACCGCACAGATGATGCTCGACGATCCTGa < 1:692862/69‑1 (MQ=255)
tGTTTTACCGACTGGCAACAGGTTCAGGAAGATATCGAAACCGCACAGATGATGCTCGATGATCCTGa < 1:1569639/68‑1 (MQ=255)
gCAACAGGTTCAGGAAGATATCGAAACCGCACAGATGATGCTCGATGATCCTGAAATGCGTga > 1:3353357/1‑63 (MQ=255)
gCAACAGGTTCAGGAAGATATAGAAACCGCACAGATGATGCTCGATGATCCTGAAATGCGTga > 1:2788263/1‑63 (MQ=255)
ttCAGGAAGATATCGAAACCGCACAGATGATGCTCGATGATCCTGAAATGCGTGAGATGGCGCAGGATg < 1:1045175/69‑1 (MQ=255)
ttCAGGAAGATATCGAAACCGCACAGATGATGCTCGATGATCCTGAAATGCGTGAGATGGCGCAGGATg < 1:2683527/69‑1 (MQ=255)
gAAGATATCGAAACCGCACAGATGATGCTCGATGATCCTGAAATGCGTGAGATGGCGCAGGATGAACTg < 1:2845085/69‑1 (MQ=255)
gAAGATATCGAAACCGCACAGATGATGCTCGACGATCCTGAAATGCGTGAGATGGCGCAGGATGAACTg < 1:972262/69‑1 (MQ=255)
gAAGATATCGAAACCGCACAGATGATGCTCGACGATCCTGAAATGCGTGAGATGGCGCAGGATGAACTg < 1:2358678/69‑1 (MQ=255)
gAAGATATCGAAACCGCACAGATGATGCTCGACGATCCTGAAATGCGTGAGATGGCGCAGGATGAACTg < 1:768584/69‑1 (MQ=255)
gAAGATATCGAAACCGCACAGATGATGCTCGACGATCCTGAAATGCGTGAGATGGCGCAGGATGAACTg < 1:2387392/69‑1 (MQ=255)
gAAGATATCGAAACCGCACAGATGATGCTCGACGATCCTGAAATGCGTGAGATGGCGCAGGATGAACTg < 1:2575815/69‑1 (MQ=255)
gAAGATATCGAAACCGCACAGATGATGCTCGACGATCCTGAAATGCGTGAGATGGCGCAGGATGAACTg < 1:672414/69‑1 (MQ=255)
gAAGATATCGAAACCGCACAGATGATGCTCGACGATCCTGAAATGCGTGAGATGGCGCAGGATGAACTg < 1:2853219/69‑1 (MQ=255)
gAAGATATCGAAACCGCACAGATGATGCTCGACGATCCTGAAATGCGTGAGATGGCGCAGGATGAACTg < 1:399397/69‑1 (MQ=255)
gAAGATATCGAAACCGCACAGATGATGCTCGACGATCCTGAAATGCGTGAGATGGCGCAGGATGAACTg < 1:3067172/69‑1 (MQ=255)
gAAGATATCGAAACCGCACAGATGATGCTCGACGATCCTGAAATGCGTGAGATGGCGCAGGATGAACTg < 1:3130503/69‑1 (MQ=255)
gAAGATATCGAAACCGCACAGATGATGCTCGACGATCCTGAAATGCGTGAGATGGCGCAGGATGAACTg < 1:1186842/69‑1 (MQ=255)
gAAGATATCGAAACCGCACAGATGATGCTCGACGATCCTGAAATGCGTGAGATGGCGCAGGATGAACTg < 1:1524411/69‑1 (MQ=255)
gAAGATATCGAAACCGCACAGATGATGCTCGACGATCCTGAAATGCGTGAGATGGCGCAGGATGAACTg < 1:1529481/69‑1 (MQ=255)
gAAGATATCGAAACCGCACAGATGATGCTCGACGATCCTGAAATGCGTGAGATGGCGCAGGATGAACTg < 1:1550912/69‑1 (MQ=255)
aaGATATCGAAACCGCACAGATGATGCTCGACGATCCTGAAATGCGTGAGATGGCGCAGGATGAACTg < 1:3065529/68‑1 (MQ=255)
aaGATATCGAAACCGCACAGATGATGCTCGACGATCCTGAAATGCGTGAGATCGCGCAGGATGAACTg < 1:3133367/68‑1 (MQ=255)
aGATATCGAAACCGCACAGATGATGCTCGATGATCCTGAAATGCGTGAGATGGCGCAGGATg < 1:1054113/62‑1 (MQ=255)
aGATATCGAAACCGCACAGATGATGCTCGATGATCCTGAAATGCGTGAGATGGCGCAGGATg < 1:769050/62‑1 (MQ=255)
aGATATCGAAACCGCACAGATGATGCTCGATGATCCTGAAATGCGTGAGATGGCGCAGGATg < 1:1461765/62‑1 (MQ=255)
aGATATCGAAACCGCACAGATGATGCTCGATGATCCTGAAATGCGTGAGATGGCGCAGGATg < 1:280706/62‑1 (MQ=255)
aGATATCGAAACCGCACAGATGATGCTCGATGATCCTGAAATGCGTGAGATGGCGCAGGATg < 1:2081704/62‑1 (MQ=255)
aGATATCGAAACCGCACAGATGATGCTCGATGATCCTGAAATGCGTGAGATGGCGCAGGATg < 1:238825/62‑1 (MQ=255)
atatCGAAACCGCACAGATGATGCTCGATGATCCTGAAATGCGTGAGATGGCGCAGGATGAACTGcgcg < 1:351885/69‑1 (MQ=255)
atatCGAAACCGCACAGATGATGCTCGATGATCCTGAAATGCGTGAGATGGCGCAGGATGAACTGcgcg < 1:1274191/69‑1 (MQ=255)
atatCGAAACCGCACAGATGATGCTCGATGATCCTGAAATGCGTGAGATGGCGCAGGATGAACTGcgcg < 1:3233761/69‑1 (MQ=255)
atatCGAAACCGCACAGATGATGCTCGATGATCCTGAAATGCGTGAGATGGCGCAGGATGAACTGcgcg < 1:1853658/69‑1 (MQ=255)
atatCGAAACCGCACAGATGATGCTCGACGATCCTGa < 1:740647/37‑1 (MQ=255)
tatCGAAACCGCACAGATGATGCTCGATGATCCTGAAATGCGTGAGATGGCGCAGGATGAACTGCGCGa < 1:3014912/69‑1 (MQ=255)
tatCGAAACCGCACAGATGATGCTCGATGATCCTGAAATGCGTGAGATGGCGCAGGATGAACTGCGCGa < 1:3012188/69‑1 (MQ=255)
tatCGAAACCGCACAGATGATGCTCGATGATCCTGAAATGCGTGAGATGGCGCAGGATGAACTGCGCGa > 1:2008554/1‑69 (MQ=255)
tatCGAAACCGCACAGATGATGCTCGATGATCCTGAAATGCGTGAGATGGCGCAGGATGAACTGCGCGa < 1:1186089/69‑1 (MQ=255)
tatCGAAACCGCACAGATGATGCTCGATGATCCTGAAATGCGTGAGATGGCGCAGGATGAACTGCGCGa < 1:3301938/69‑1 (MQ=255)
tatCGAAACCGCACAGATGATGCTCGATGATCCTGAAATGCGTGAGATGGCGCAGGATGAACTGCGCGa < 1:2643742/69‑1 (MQ=255)
tatCGAAACCGCACAGATGATGCTCGATGATCCTGAAATGCGTGAGATGGCGCAGGATGAACTGCGCGa > 1:3305161/1‑69 (MQ=255)
tatCGAAACCGCACAGATGATGCTCGATGATCCTGAAATGCGTGAGATGGCGCAGGATGAACTGCGCGa < 1:3337347/69‑1 (MQ=255)
tatCGAAACCGCACAGATGATGCTCGATGATCCTGAAATGCGTGAGATGGCGCAGGATGAACTGCGCGa < 1:67195/69‑1 (MQ=255)
tatCGAAACCGCACAGATGATGATCGATGATCCTGAAATGCGTGAGATGGCGCAGGATGAACTGCGCGa > 1:2160115/1‑69 (MQ=255)
atCGAAACCGCACAGATGATGCTCGATGATCCTGAAATGCGTGAGATGGCGCAGGATGAACTGCGCGa < 1:525107/68‑1 (MQ=255)
gAAACCGCACAGATGATGCTCGATGATCCTGAAATGCGTGAGATGGCGCAGGATGAACTGCGCGAAGCt < 1:1426760/69‑1 (MQ=255)
gAAACCGCACAGATGATGCTCGATGATCCTGAAATGCGTGAGATGGCGCAGGATGAACTGCGCGAAGCt < 1:1320835/69‑1 (MQ=255)
gAAACCGCACAGATGATGCTCGATGATCCTGAAATGCGTGAGATGGCGCAGGATGAACTGCGCGAAGCt < 1:2078572/69‑1 (MQ=255)
gAAACCGCACAGATGATGCTCGATGATCCTGAAATGCGTGAGATGGCGCAGGATGAACTGCGCGAAGCt < 1:115989/69‑1 (MQ=255)
gAAACCGCACAGATGATGCTCGATGATCCTGAAATGCGTGAGATGGCGCAGGATGAACTGCGCGAAGCt < 1:1996942/69‑1 (MQ=255)
gAAACCGCACAGATGATGCTCGATGATCCTGAAATGCGTGAGATGGCGCAGGATGAACTGCGCGAAGCt < 1:2596371/69‑1 (MQ=255)
gAAACCGCACAGATGATGCTCGATGATCCTGAAATGCGTGAGATGGCGCAGGATGAACTGCGCGAAGCt < 1:2610073/69‑1 (MQ=255)
gAAACCGCACAGATGATGCTCGACGATCCTGAAATGCGTGAGATGGCGCAGGATGAACTGCGCGAAGc > 1:2392748/1‑68 (MQ=255)
gAAACCGCACAGATGATGCTCGACGATCCTGAAATGCGTGAGATGGCGCAGGATGAACTGCGCGAAGc > 1:3012106/1‑68 (MQ=255)
gAAACCGCACAGATGATGCTCGACGATCCTGAAATGCGTGAGATGGCGCAGGATGAACTGCGCGAAGc > 1:1396947/1‑68 (MQ=255)
gAAACCGCACAGATGATGCTCGACGATCCTGAAATGCGTGAGATGGCGCAGGATGAACTGCGCGAAGc > 1:2906302/1‑68 (MQ=255)
gAAACCGCACAGATGATGCTCGACGATCCTGAAATGCGTGAGATGGCGCAGGATGAACTGCGCGAAGc > 1:167987/1‑68 (MQ=255)
gAAACCGCACAGATGATGCTCGACGATCCTGAAATGCGTGAGATGGCGCAGGATGAACTGCGCGAAGc > 1:1684350/1‑68 (MQ=255)
gAAACAGCACAGATGATGCTCGATGATCCTGAAATGCGTGAGATGGCGCAGGATGAACTGCGCGAAGCt < 1:681798/69‑1 (MQ=255)
cGCACAGATGATGCTCGATGATCCTGAAATGCGTGAGATGGCGCAGGATGAACTGCGCGAAGCTAAAg > 1:2142165/1‑68 (MQ=255)
cGCACAGATGATGCTCGATGATCCTGAAATGCGTGAGATGGCGCAGGATGAACTGCGCGAAGCTAAAg > 1:3277861/1‑68 (MQ=255)
atgatgCTCGATGATCCTGAAATGCGTGAGATGGCGCAGGATGAACTGCGCGAAGCTAAAGaaaaaa > 1:2661044/1‑67 (MQ=255)
atgatgCTCGATGATCCTGAAATGCGTGAGATGGCGCAGGATGAACTGCGCGAAGCTAAAGaaaaaa > 1:2449889/1‑67 (MQ=255)
atgCTCGATGATCCTGAAATGCGTGAGATGGCGCAGGATGAACTGCGCGAAGCTAAAGTAAAAAGCg < 1:364902/67‑1 (MQ=255)
atgCTCGATGATCCTGAAATGCGTGAGATGGCGCAGGATGAACTGCGCGAAGCTAAAGAAAAAAGc > 1:950939/1‑66 (MQ=255)
atgCTCGATGATCCTGAAATGCGTGAGATGGCGCAGGATGAACTGCGCGAAGCTAAAGAAAAAAGCg < 1:1293680/67‑1 (MQ=255)
atgCTCGATGATCCTGAAATGCGTGAGATGGCGCAGGATGAACTGCGCGAAGCTAAAGAAAAAAGCg > 1:2082651/1‑67 (MQ=255)
atgCTCGATGATCCTGAAATGCGTGAGATGGCGCAGGATGAACTGCGCGAAGCTAAAGAAAAAAGCg > 1:1206606/1‑67 (MQ=255)
atgCTCGATGATCCTGAAATGCGTGAGATGGCGCAGGATGAACTGCGCGAAGCTAAAGAAAAAAGCg < 1:243630/67‑1 (MQ=255)
atgCTCGATGATCCTGAAATGCGTGAGATGGCGCAGGATGAACTGCGCGAAGCTAAAGAAAAAAGCg > 1:140514/1‑67 (MQ=255)
atgCTCGATGATCCTGAAATGCGTGAGATGGCGCAGGATGAACTGCGCGAAGCTAAAGAAAAAAGCg > 1:1452431/1‑67 (MQ=255)
atgCTCGATGATCCTGAAATGCGTGAGATGGCGCAGGATGAACTGCGCGAAGCTAAAGAAAAAAGCg < 1:220786/67‑1 (MQ=255)
atgCTCGATGATCCTGAAATGCGTGAGATGGCGCAGGATGAACTGCGCGAAGCTAAAGAAAAAAGCGa > 1:459576/1‑68 (MQ=255)
atgCTCGATGATCCTGAAATGCGTGAGATGGCGCAGGATGAACTGCGCGAAGCTAAAGAAAAAAGCGa > 1:353303/1‑68 (MQ=255)
atgCTCGATGATCCTGAAATGCGTGAGATGGCGCAGGATGAACTGCGCGAAGCTAAAGAAAAAAGCGa > 1:2285030/1‑68 (MQ=255)
gCTCGATGATCCTGAAATGCGTGAGATGGCGCAGGATGAACTGCGCGAAGCTAAAGAAAAAAGCGAGc < 1:317890/68‑1 (MQ=255)
gCTCGATGATCCTGAAATGCGTGAGATGGCGCAGGATGAACTGCGCGAAGCTAAAGAAAAAAGCGAGc < 1:1960740/68‑1 (MQ=255)
cGATGATCCTGAAATGCGTGAGATGGCGCAGGATGAACTGCGCGAAGCTAAAGAAAAAAGCGAGCAAc > 1:2330143/1‑68 (MQ=255)
cGATGATCCTGAAATGCGTGAGATGGCGCAGGATGAACTGCGCGAAGCTAAAGAAAAAAGCGAGCAAc > 1:1643984/1‑68 (MQ=255)
cGATGATCCTGAAATGCGTGAGATGGCGCAGGATGAACTGCGCGAAGCTAAAGAAAAAAGCGAGCAAc > 1:1751039/1‑68 (MQ=255)
cGATGATCCTGAAATGCGTGAGATGGCGCAGGATGAACTGCGCGAAGCTAAAGAAAAAAGCGAGCAAc > 1:1947190/1‑68 (MQ=255)
tgatCCTGAAATGCGTGAGATGGCGCAGGATGAACTGCGCGAAGCTAAAGAAAAAAGCGAGCAACTgg < 1:3231040/68‑1 (MQ=255)
tgatCCTGAAATGCGTGAGATGGCGCAGGATGAACTGCGCGAAGCTAAAGAAAAAAGCGAGCAACTgg < 1:3148373/68‑1 (MQ=255)
tgatCCTGAAATGCGTGAGATGGCGCAGGATGAACTGCGCGAAGCTAAAGAAAAAAGCGAGCAACTgg < 1:900633/68‑1 (MQ=255)
|
CTGTTTTACCGACTGGCAACAGGTTCAGGAAGATATCGAAACCGCACAGATGATGCTCGATGATCCTGAAATGCGTGAGATGGCGCAGGATGAACTGCGCGAAGCTAAAGAAAAAAGCGAGCAACTGG > minE/817375‑817502
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
