Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F1 I0 R1
|
4577 |
57.4 |
3436498 |
77.7% |
2670158 |
63.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
828,872 |
G→A |
35.7% |
D142D (GAC→GAT) |
narX ← |
sensory histidine kinase in two‑component regulatory system with NarL |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 828,872 | 0 | G | A | 35.7%
| 54.2
/ 47.1
| 81 | D142D (GAC→GAT) | narX | sensory histidine kinase in two‑component regulatory system with NarL |
| Reads supporting (aligned to +/- strand): ref base G (25/27); new base A (21/8); total (46/35) |
| Fisher's exact test for biased strand distribution p-value = 3.86e-02 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.68e-01 |
AATACCGCCATTACCCGATGGACCAGTACCACTGTCTCGATGCGCATTTCCGTGGTGCGGTCAAAACCAGATACCAGTTGATCAAGCCCGGCAACAAACTGGCTGACATCCGCTGACAC > minE/828813‑828931
|
aaTACCGCCATTACCCGATGTACCAGTACCACTGTCTCGATGCGCATTTCCGTGGTGCGGTCaaa > 1:544883/1‑65 (MQ=255)
aaTACCGCCATTACCCGATGGGCCAGCACCACTGTCTCAATGCGCATTTCCGTGGTGCGATCAAAACCa < 1:372156/69‑1 (MQ=255)
aaTACCGCCATTACCCGATGGACCAGTACCACTGTCTCGATGCGCATTTCCGTGGTGCGGTCaaa > 1:959331/1‑65 (MQ=255)
aaTACCGCCATTACCCGATGGACCAGTACCACTGTCTCGATGCGCATTTCCGTGGTGCGGTCaaa > 1:1031696/1‑65 (MQ=255)
aaTACCGCCATTACCCGATGGACCAGTACCACTGTCTCGATGCGCATTTCCGTGGTGCGGTCaaa > 1:1043842/1‑65 (MQ=255)
aaTACCGCCATTACCCGATGGACCAGTACCACTGTCTCGATGCGCATTTCCGTGGTGCGGTCaaa > 1:910841/1‑65 (MQ=255)
aaTACCGCCATTACCCGATGGACCAGTACCACTGTCTCGATGCGCATTTCCGTGGTGCGGTCaaa > 1:906150/1‑65 (MQ=255)
aaTACCGCCATTACCCGATGGACCAGTACCACTGTCTCGATGCGCATTTCCGTGGTGCGGTCaaa > 1:728700/1‑65 (MQ=255)
aaTACCGCCATTACCCGATGGACCAGTACCACTGTCTCGATGCGCATTTCCGTGGTGCGGTCaaa > 1:712874/1‑65 (MQ=255)
aaTACCGCCATTACCCGATGGACCAGTACCACTGTCTCGATGCGCATTTCCGTGGTGCGGTCaaa > 1:1582046/1‑65 (MQ=255)
aaTACCGCCATTACCCGATGGACCAGTACCACTGTCTCGATGCGCATTTCCGTGGTGCGGTCaaa > 1:2324725/1‑65 (MQ=255)
aaTACCGCCATTACCCGATGGACCAGTACCACTGTCTCGATGCGCATTTCCGTGGTGCGGTCAAAACCa < 1:2001434/69‑1 (MQ=255)
aaTACCGCCATTACCCGATGGACCAGTACCACTGTCTCGATGCGCATTTCCGTGGTGCGGTCAAAACCa < 1:1025059/69‑1 (MQ=255)
aaTACCGCCATTACCCGATGGACCAGTACCACTGTCTCGATGCGCATTTCCGTGGTGCGGTCAAAACCa < 1:2149395/69‑1 (MQ=255)
aaTACCGCCATTACCCGATGGACCAGTACCACTGTCTCGATGCGCATTTCCGTGGTGCGGTCAAAACCa < 1:23023/69‑1 (MQ=255)
aaTACCGCCATTACCCGATGGACCAGTACCACTGTCTCGATGCGCATTTCCGTGGTGCGGTCAAAACCa < 1:2951080/69‑1 (MQ=255)
aaTACCGCCATTACCCGATGGACCAGTACCACTGTCTCGATGCGCATTTCCGTGGTGCGGTCAAAACCa < 1:456633/69‑1 (MQ=255)
aaTACCGCCATTACCCGATGGACCAGTACCACTGTCTCGATGCGCATTTCCGTGGTGCGGTCAAAACCa < 1:498404/69‑1 (MQ=255)
aaTACCGCCATTACCCGATGGACCAGTACCACTGTCTCGATGCGCATTTCCGTGGTGCGGTCAAAACCa < 1:1301016/69‑1 (MQ=255)
aaTACCGCCATTACCCGATGGACCAGTACCACTGTCTCGATGCGCATTTCCGTGGTGCGGTCAAAACCa < 1:1274542/69‑1 (MQ=255)
aaTACCGCCATTACCCGATGGACCAGTACCACTGTCTCGATGCGCATTTCCGTGGTGCGGTCAAAACCa < 1:1091536/69‑1 (MQ=255)
aaTACCGCCATTACCCGATGGACCAGTACCACCGTCTCGATGCGCATTTCCGTGGTGCGGTCaaa > 1:1198166/1‑65 (MQ=255)
aaTACCGCCATTACCCGATGGACCAGCACCACTGTCTCAATGCGCATTTCCGTGGTGCGATCAAAACCa < 1:590732/69‑1 (MQ=255)
aaTACCGCCATTACCCGATGGACCAGCACCACTGTCTCAATGCGCATTTCCGTGGTGCGATCAAAACCa < 1:3410403/69‑1 (MQ=255)
aaTACCGCCATTACCCGATGGACCAGCACCACTGTCTCAATGCGCATTTCCGTGGTGCGATCAAAACCa < 1:818402/69‑1 (MQ=255)
aaTACCGCCATTACCCGATGGACCAGCACCACTGTCTCAATGCGCATTTCCGTGGTGCGATCAAAACCa < 1:2758094/69‑1 (MQ=255)
aaTACCGCCATTACCCGATGGACCAGCACCACTGTCTCAATGCGCATTTCCGTGGTGCGATCAAAACCa < 1:2393523/69‑1 (MQ=255)
aaTACCGCCATTACCCGATGGACCAGCACCACTGTCTCAATGCGCATTTCCGTGGTGCGATCAAAACCa < 1:1490870/69‑1 (MQ=255)
aaTACCGCCATTACCCGATGGACCAGCACCACTGTCTCAATGCGCATTTCCGTGGTGCGATCAAAACCa < 1:156445/69‑1 (MQ=255)
tACCGCCATTACCCGATGGACCAGTACCACTGTCTCGATGCGCATTTCCGTGGTGCGGTCAAAACCAGa > 1:842809/1‑69 (MQ=255)
ccACTGTCTCGATGCGCATTTCCGTGGTGCGGTCAAAACCAGATACCAGTTGATCAAGCCCGGcaacaa > 1:1261007/1‑69 (MQ=255)
ccACTGTCTCGATGCGCATTTCCGTGGTGCGGTCAAAACCAGATACCAGTTGATCAAGCCCGGcaacaa > 1:2761735/1‑69 (MQ=255)
tctcGATGCGCATTTCCGTGGTGCGGTCAAAACCa < 1:3385662/35‑1 (MQ=255)
caaTGCGCATTTCCGTGGTGCGATCAAAACCAGATACCAGCTGATCAAGCCCGGCAACAAACTGGc > 1:387007/3‑66 (MQ=255)
caaTGCGCATTTCCGTGGTGCGATCAAAACCAGATACCAGCTGATCAAGCCCGGCAACAAACTGGCt > 1:2189712/3‑67 (MQ=255)
caaTGCGCATTTCCGTGGTGCGATCAAAACCAGATACCAGCTGATCAAGCCCGGCAACAAACTGGCt > 1:395204/3‑67 (MQ=255)
caaTGCGCATTTCCGTGGTGCGATCAAAACCAGATACCAGCTGATCAAGCCCGGCAACAAACTGGCt > 1:390171/3‑67 (MQ=255)
caaTGCGCATTTCCGTGGTGCGATCAAAACCAGATACCAGCTGATCAAGCCCGGCAACAAACTGGCt > 1:1661839/3‑67 (MQ=255)
caaTGCGCATTTCCGTGGTGCGATCAAAACCAGATACCAGCTGATCAAGCCCGGCAACAAACTGGCt > 1:652/3‑67 (MQ=255)
caaTGCGCATTTCCGTGGTGCGATCAAAACCAGATACCAGCTGATCAAGCCCGGCAACAAACTGGCt > 1:1237648/3‑67 (MQ=255)
caaTGCGCATTTCCGTGGTGCGATCAAAACCAGATACCAGCTGATCAAGCCCGGCAACAAACTGGCt > 1:481498/3‑67 (MQ=255)
caaTGCGCATTTCCGTGGTGCGATCAAAACCAGATACCAGCTGATCAAGCCCGGCAACAAACTGGCt > 1:3226876/3‑67 (MQ=255)
caaTGCGCATTTCCGTGGTGCGATCAAAACCAGATACCAGCTGATCAAGCCCGGCAACAAACTGGCt > 1:2956849/3‑67 (MQ=255)
caaTGCGCATTTCCGTGGTGCGATCAAAACCAGATACCAGCTGATCAAGCCCGGCAACAAACTGGCt > 1:1462493/3‑67 (MQ=255)
caaTGCGCATTTCCGTGGTGCGATCAAAACCAGATACCAGCTGATCAAGCCCGGCAACAAACTGGCt > 1:280329/3‑67 (MQ=255)
caaTGCGCATTTCCGTGGTGCGATCAAAACCAGATACCAGCTGATCAAGCCCGGCAACAAACTGGCt > 1:2214000/3‑67 (MQ=255)
caaTGCGCATTTCCGTGGTGCGATCAAAACCAGATACCAGCTGATCAAGCCCGGCAACAAACTGGCt > 1:520867/3‑67 (MQ=255)
caaTGCGCATTTCCGTGGTGCGATCAAAACCAGATACCAGCTGATCAAGCCCGGCAACAAACTGGCt > 1:1914636/3‑67 (MQ=255)
caaTGCGCATTTCCGTGGTGCGATCAAAACCAGATACCAGCTGATCAAGCCCGGCAACAAACTGGCt > 1:907139/3‑67 (MQ=255)
caaTGCGCATTTCCGTGGTGCGATCAAAACCAGATACCAGCTGATCAAGCCCGGCAACAAACTGGCt > 1:1968924/3‑67 (MQ=255)
caaTGCGCATTTCCGTGGTGCGATCAAAACCAGATACCAGCTGATCAAGCCCGGCAACAAACTGGCt > 1:2488755/3‑67 (MQ=255)
caaTGCGCATTTCCGTGGTGCGATCAAAACCAGATACCAGCTGATCAAGCCCGGCAACAAACTGGCt > 1:196919/3‑67 (MQ=255)
caaTGCGCATTTCCGTGGTGCGATCAAAACCAGATACCAGCTGATCAAGCCCGGCAACAAACTGGCt > 1:2370810/3‑67 (MQ=255)
caaTGCGCATTTCCGTGGTGCGATCAAAACCAGATACCAGCTGATCAAGCCCGGCAACAAACTGGCt > 1:1614491/3‑67 (MQ=255)
tCCGTGGTGCGGTCAAAACCAGATACCAGTTGATCAAGCCCGGCAACAAACTGGCTGAc > 1:1818002/1‑59 (MQ=255)
tCCGTGGTGCGGTCAAAACCAGATACCAGTTGATCAAGCCCGGCAACAAACTGGCTGACa > 1:2907663/1‑60 (MQ=255)
tCCGTGGTGCGGTCAAAACCAGATACCAGTTGATCAAGCCCGGCAACAAACTGGCTGACa > 1:1418012/1‑60 (MQ=255)
tCCGTGGTGCGGTCAAAACCAGATACCAGTTGATCAAGCCCGGCAACAAACTGGCTGACa > 1:2340030/1‑60 (MQ=255)
tCCGTGGTGCGGTCAAAACCAGATACCAGTTGATCAAGCCCGGCAACAAACTGGCTGACa > 1:2665768/1‑60 (MQ=255)
tCCGTGGTGCGGTCAAAACCAGATACCAGTTGATCAAGCCCGGCAACAAACTGGCTGACa > 1:1884907/1‑60 (MQ=255)
tCCGTGGTGCGGTCAAAACCAGATACCAGTTGATCAAGCCCGGCAACAAACTGGCTGACATCCGCTGac > 1:169605/1‑69 (MQ=255)
tCCGTGGTGCGGTCAAAACCAGATACCAGTTGATCAAGCCCGGCAACAAACTGGCTGACATCCGCTGac > 1:649190/1‑69 (MQ=255)
tCCGTGGTGCGGTCAAAACCAGATACCAGTTGATCAAGCCCGGCAACAAACTGGCTGACATCCGCTGac > 1:1235025/1‑69 (MQ=255)
tCCGTGGTGCGGTCAAAACCAGATACCAGTTGATCAAGCCCGGCAACAAACTGGCTGACATCCGCTGac > 1:761797/1‑69 (MQ=255)
tCCGTGGTGCGGTCAAAACCAGATACCAGTTGATCAAGCCCGGCAACAAACTGGCTGACATCCGCTGac > 1:2284958/1‑69 (MQ=255)
gtggtgCGGTCAAAACCAGATACCAGTTGATCAAGCCCGGCAACAAACTGGCTGACATCCGCTGacac < 1:832267/68‑1 (MQ=255)
gtggtgCGGTCAAAACCAGATACCAGTTGATCAAGCCCGGCAACAAACTGGCTGACATCCGCTGacac < 1:640613/68‑1 (MQ=255)
gtggtgCGGTCAAAACCAGATACCAGTTGATCAAGCCCGGCAACAAACTGGCTGACATCCGCTGacac < 1:1019945/68‑1 (MQ=255)
gtggtgCGGTCAAAACCAGATACCAGTTGATCAAGCCCGGCAACAAACTGGCTGACATCCGCTGacac < 1:534655/68‑1 (MQ=255)
gtggtgCGGTCAAAACCAGATACCAGTTGATCAAGCCCGGCAACAAACTGGCTGACATCCGCTGacac < 1:462992/68‑1 (MQ=255)
gtggtgCGGTCAAAACCAGATACCAGTTGATCAAGCCCGGCAACAAACTGGCTGACATCCGCTGacac < 1:3406964/68‑1 (MQ=255)
gtggtgCGGTCAAAACCAGATACCAGTTGATCAAGCCCGGCAACAAACTGGCTGACATCCGCTGacac < 1:3395031/68‑1 (MQ=255)
gtggtgCGGTCAAAACCAGATACCAGTTGATCAAGCCCGGCAACAAACTGGCTGACATCCGCTGacac < 1:3327939/68‑1 (MQ=255)
gtggtgCGGTCAAAACCAGATACCAGTTGATCAAGCCCGGCAACAAACTGGCTGACATCCGCTGacac < 1:3021768/68‑1 (MQ=255)
gtggtgCGGTCAAAACCAGATACCAGTTGATCAAGCCCGGCAACAAACTGGCTGACATCCGCTGacac < 1:2945437/68‑1 (MQ=255)
gtggtgCGGTCAAAACCAGATACCAGTTGATCAAGCCCGGCAACAAACTGGCTGACATCCGCTGacac < 1:2795371/68‑1 (MQ=255)
gtggtgCGGTCAAAACCAGATACCAGTTGATCAAGCCCGGCAACAAACTGGCTGACATCCGCTGacac < 1:2661363/68‑1 (MQ=255)
gtggtgCGGTCAAAACCAGATACCAGTTGATCAAGCCCGGCAACAAACTGGCTGACATCCGCTGacac < 1:2535423/68‑1 (MQ=255)
gtggtgCGGTCAAAACCAGATACCAGTTGATCAAGCCCGGCAACAAACTGGCTGACATCCGCTGacac < 1:1492151/68‑1 (MQ=255)
gtggtgCGGTCAAAACCAGATACCAGTTGATCAAGCCCGGCAACAAACTGGCTGACATCCGCTGacac < 1:1704726/68‑1 (MQ=255)
tggtgCGGTCAAAACCAGATACCAGTTGATCAAGCCCGGCAACAAACTGGCTGACATCCGCTGacac < 1:2128502/67‑1 (MQ=255)
|
AATACCGCCATTACCCGATGGACCAGTACCACTGTCTCGATGCGCATTTCCGTGGTGCGGTCAAAACCAGATACCAGTTGATCAAGCCCGGCAACAAACTGGCTGACATCCGCTGACAC > minE/828813‑828931
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
