Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F1 I0 R1
|
4577 |
57.4 |
3436498 |
77.7% |
2670158 |
63.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
834,062 |
C→T |
51.8% |
G840G (GGC→GGT) |
narG → |
nitrate reductase 1, alpha subunit |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 834,062 | 0 | C | T | 51.8%
| ‑2.2
/ 72.8
| 81 | G840G (GGC→GGT) | narG | nitrate reductase 1, alpha subunit |
| Reads supporting (aligned to +/- strand): ref base C (18/21); new base T (26/16); total (44/37) |
| Fisher's exact test for biased strand distribution p-value = 1.84e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.14e-01 |
AAGCGAAAAGCGACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCATGACTCTGCCGCTGAA > minE/833998‑834122
|
aaGCGAAAAGCGACTGTGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTTGGTCAt < 1:2179751/68‑1 (MQ=255)
aaGCGAAAAGCGACTGGGAAATCTACAAAGCCATCGCTAAGAAATTCTCCGAAGTGTGCGTTGGTCAt < 1:373424/68‑1 (MQ=255)
aaGCGAAAAGCGACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTTGGTCAt < 1:1972254/68‑1 (MQ=255)
aaGCGAAAAGCGACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTTGGTCAt < 1:2002614/68‑1 (MQ=255)
aaGCGAAAAGCGACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTTGGTCAt < 1:1636959/68‑1 (MQ=255)
aaGCGAAAAGCGACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTTGGTCAt < 1:2572561/68‑1 (MQ=255)
aaGCGAAAAGCGACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTTGGTCAt < 1:3048937/68‑1 (MQ=255)
aaGCGAAAAGCGACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTTGGTCAt < 1:2517925/68‑1 (MQ=255)
aaGCGAAAAGCGACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTTGGTCAt < 1:144576/68‑1 (MQ=255)
aaGCGAAAAGCGACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTTGGTCAt < 1:3411540/68‑1 (MQ=255)
aaGCGAAAAGCGACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTTGGTCAt < 1:442802/68‑1 (MQ=255)
aaGCGAAAAGCGACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTTGGTCAt < 1:484687/68‑1 (MQ=255)
aaGCGAAAAGCGACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTTGGTCAt < 1:2459416/68‑1 (MQ=255)
aaGCGAAAAGCGACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTTGGTCAt < 1:827457/68‑1 (MQ=255)
aaGCGAAAAGCGACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTTGGTCAt < 1:2323190/68‑1 (MQ=255)
aaGCGAAAAGCGACTGGGAAATCTACAAAGCCATCGCGAACAAATTCTCCGAAGTGTGCGTTGGTCAt < 1:2515061/68‑1 (MQ=255)
tCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTTGGTCATc > 1:3280213/1‑48 (MQ=255)
tCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTTGGTCATCTGGGTAAAGAAAGTGACATc > 1:3391382/1‑68 (MQ=255)
tCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTTGGTCATCTGGGTAAAGAAACTGACATc > 1:2616834/1‑68 (MQ=255)
tCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTTGGTCATCTGGGTAAAGAAACTGACATc > 1:2341592/1‑68 (MQ=255)
tCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTTGGTCATCTGGGTAAAGAAACTGACATc > 1:2800266/1‑68 (MQ=255)
tCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTTGGTCATCTGGGTAAAGAAACTGACATc > 1:2908151/1‑68 (MQ=255)
tCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTTGGTCATCTGGGTAAAGAAACTGACATc > 1:2880597/1‑68 (MQ=255)
tCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTTGGTCATCTGGGTAAAGAAACTGACATc > 1:289969/1‑68 (MQ=255)
tCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTTGGTCATCTGGGTAAAGAAACTGACATc > 1:2900156/1‑68 (MQ=255)
tCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTTGGTCATCTGGGTAAAGAAACTGACATc > 1:938117/1‑68 (MQ=255)
tCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTTGGTCATCTGGGTAAAGAAACTGACATc > 1:3155589/1‑68 (MQ=255)
tCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTTGGTCATCTGGGTAAAGAAACTGACATc > 1:519947/1‑68 (MQ=255)
tCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTTGGTCATCTGGGTAAAGAAACTGACATc > 1:808160/1‑68 (MQ=255)
tCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTTGGTCATCTGGGTAAAGAAACTGACATc > 1:90610/1‑68 (MQ=255)
tCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTTGGTCATCTGGGTAAAGAAACTGACATc > 1:934978/1‑68 (MQ=255)
tCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTTGGTCATCTGGGTAAAGAAACTGACATc > 1:1057876/1‑68 (MQ=255)
tCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTTGGTCATCTGGGTAAAGAAACTGACATc > 1:2088851/1‑68 (MQ=255)
tCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTTGGTCATCTGGGTAAAGAAACTGACATc > 1:1173125/1‑68 (MQ=255)
tCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTTGGTCATCTGGGTAAAGAAACTGACATc > 1:1196444/1‑68 (MQ=255)
tCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTTGGTCATCTGGGTAAAGAAACTGACATc > 1:1371294/1‑68 (MQ=255)
tCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTTGGTCATCTGGGTAAAGAAACTGACATc > 1:1462947/1‑68 (MQ=255)
tCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTTGGTCATCTGGGTAAAGAAACTGACATc > 1:1897517/1‑68 (MQ=255)
tCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTTGGTCATCTGGGTAAAGAAACTGACATc > 1:1973169/1‑68 (MQ=255)
tCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTTGGTCATCTGGGTAAAGAAACTGACATc > 1:204195/1‑68 (MQ=255)
tCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTTGGTCATCTGGGTAAAGAAACTGACATc > 1:2045779/1‑68 (MQ=255)
tCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTAGGTCATCTGGGTAAAGAAACTGACATc > 1:1064542/1‑68 (MQ=255)
cTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGt < 1:2550409/69‑1 (MQ=255)
cTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGt < 1:1068048/69‑1 (MQ=255)
cTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGt < 1:671584/69‑1 (MQ=255)
cTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGt < 1:667372/69‑1 (MQ=255)
cTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGt < 1:1740803/69‑1 (MQ=255)
cTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGGCGGCCATCTGGGTAAAGAAACCGACATCGt < 1:3277857/69‑1 (MQ=255)
gcgAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTa > 1:2111572/1‑67 (MQ=255)
gcgAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTa > 1:792549/1‑67 (MQ=255)
gcgAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTa > 1:599474/1‑67 (MQ=255)
gcgAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTa > 1:1314562/1‑67 (MQ=255)
gcgAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTa > 1:478556/1‑67 (MQ=255)
gcgAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTa > 1:2060349/1‑67 (MQ=255)
gcgAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTa > 1:1490452/1‑67 (MQ=255)
gcgAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTa > 1:1581622/1‑67 (MQ=255)
gcgAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTa > 1:2038018/1‑67 (MQ=255)
gcgAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTa > 1:1596059/1‑67 (MQ=255)
gcgAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTa > 1:2710378/1‑67 (MQ=255)
gcgAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTa > 1:2457491/1‑67 (MQ=255)
gcgAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTa > 1:2855102/1‑67 (MQ=255)
gcgAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTa > 1:2857191/1‑67 (MQ=255)
gcgAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTa > 1:2447417/1‑67 (MQ=255)
aagaaATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAg < 1:204585/69‑1 (MQ=255)
aagaaATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAg < 1:2965654/69‑1 (MQ=255)
aagaaATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAg < 1:1333334/69‑1 (MQ=255)
aagaaATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAg < 1:1578441/69‑1 (MQ=255)
tgCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCATGACTCTGCCGCTg > 1:523068/1‑67 (MQ=255)
tgCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCATGACTCTGCCGCTGaa > 1:2262736/1‑69 (MQ=255)
tgCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCATGACTCTGCCGCTGaa > 1:2202709/1‑69 (MQ=255)
cGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGcc < 1:45671/42‑1 (MQ=255)
cGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGcc < 1:1349511/42‑1 (MQ=255)
cGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGcc < 1:2087097/42‑1 (MQ=255)
cGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGcc < 1:1223076/42‑1 (MQ=255)
cGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGcc < 1:3407953/42‑1 (MQ=255)
cGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGcc < 1:639227/42‑1 (MQ=255)
cGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGcc < 1:3406009/42‑1 (MQ=255)
cGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGcc < 1:1620945/42‑1 (MQ=255)
cGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGcc < 1:294348/42‑1 (MQ=255)
cGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGcc < 1:111287/42‑1 (MQ=255)
cGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGcc < 1:2055849/42‑1 (MQ=255)
|
AAGCGAAAAGCGACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCATGACTCTGCCGCTGAA > minE/833998‑834122
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
