Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F1 I0 R1
|
4577 |
57.4 |
3436498 |
77.7% |
2670158 |
63.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
835,957 |
T→C |
47.0% |
T225T (ACT→ACC) |
narH → |
nitrate reductase 1, beta (Fe‑S) subunit |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 835,957 | 0 | T | C | 47.0%
| 4.2
/ 72.0
| 83 | T225T (ACT→ACC) | narH | nitrate reductase 1, beta (Fe‑S) subunit |
| Reads supporting (aligned to +/- strand): ref base T (28/16); new base C (20/19); total (48/35) |
| Fisher's exact test for biased strand distribution p-value = 2.75e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.66e-01 |
AGCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGATAAATGCCGTGGCTGGCGTATGTGCATCACTGGATGCCCGTACAAAAAAATCTACTTCAACTGGAAGAGCGGTAAGTCTGAGAAGTGCATCTTCTGC > minE/835890‑836023
|
aGCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGATAAATGCCGTGGCTGGCGTATGTGCATCACTg < 1:2387346/69‑1 (MQ=255)
aGCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGATAAATGCCGTGGCTGGCGTATGTGCATCACTg < 1:632221/69‑1 (MQ=255)
aGCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGATAAATGCCGTGGCTGGCGTATGTGCATCACTg < 1:614342/69‑1 (MQ=255)
aGCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGATAAATGCCGTGGCTGGCGTATGTGCATCACTg < 1:2303718/69‑1 (MQ=255)
aGCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGATAAATGCCGTGGCTGGCGTATGTGCATCACTg < 1:1006738/69‑1 (MQ=255)
aGCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGATAAATGCCGTGGCTGGCGTATGTGCATCACTg < 1:2230782/69‑1 (MQ=255)
aagaagATGGCATCGTCCTGATCGACCAGGATAAATGCCGTGGCTGGCGTATGTGCATCACTg < 1:3034073/63‑1 (MQ=255)
gaTGGCATCGTCCTGATCGACCAGGATAAATGCCGTGGCTGGCGTATGTGCATCACTGGATGCCCGTa > 1:1260928/1‑68 (MQ=255)
gaTGGCATCGTCCTGATCGACCAGGATAAATGCCGTGGCTGGCGTATGTGCATCACTGGATGCCCGTa > 1:1268773/1‑68 (MQ=255)
gaTGGCATCGTCCTGATCGACCAGGATAAATGCCGTGGCTGGCGTATGTGCATCACTGGATGCCCGTa > 1:591325/1‑68 (MQ=255)
gaTGGCATCGTCCTGATCGACCAGGATAAATGCCGTGGCTGGCGTATGTGCATCACTGGATGCCCGTa > 1:1859093/1‑68 (MQ=255)
gTCCTGATCGACCAGGATAAATGCCGTGGCTGGCGTATGTGCATCACTGGATGCCCGTACAAAAAAAt > 1:3072649/1‑68 (MQ=255)
gTCCTGATCGACCAGGATAAATGCCGTGGCTGGCGTATGTGCATCACTGGATGCCCGTACAAAAAAATc > 1:41865/1‑69 (MQ=255)
gTCCTGATCGACCAGGATAAATGCCGTGGCTGGCGTATGTGCATCACTGGATGCCCGTACAAAAAAATc > 1:1678988/1‑69 (MQ=255)
cTGATCGACCAGGATAAATGCCGTGGCTGGCGTATGTGCATCACTgg > 1:539026/1‑47 (MQ=255)
cTGATCGACCAGGATAAATGCCGTGGCTGGCGTATGTGCATCACTGGATGCCCGTACAAAAAAAt > 1:3239597/1‑65 (MQ=255)
cTGATCGACCAGGATAAATGCCGTGGCTGGCGTATGTGCATCACTGGATGCCCGTACAAAAAAATc > 1:3210532/1‑66 (MQ=255)
cTGATCGACCAGGATAAATGCCGTGGCTGGCGTATGTGCATCACTGGATGCCCGTACAAAAAAATc > 1:2205119/1‑66 (MQ=255)
cTGATCGACCAGGATAAATGCCGTGGCTGGCGTATGTGCATCACTGGATGCCCGTACAAAAAAATc > 1:2399534/1‑66 (MQ=255)
cTGATCGACCAGGATAAATGCCGTGGCTGGCGTATGTGCATCACTGGATGCCCGTACAAAAAAATc > 1:2635207/1‑66 (MQ=255)
cTGATCGACCAGGATAAATGCCGTGGCTGGCGTATGTGCATCACTGGATGCCCGTACAAAAAAATc > 1:2474735/1‑66 (MQ=255)
cTGATCGACCAGGATAAATGCCGTGGCTGGCGTATGTGCATCACTGGATGCCCGTACAAAAAAATc > 1:445598/1‑66 (MQ=255)
cTGATCGACCAGGATAAATGCCGTGGCTGGCGTATGTGCATCACTGGATGCCCGTACAAAAAAATc > 1:996096/1‑66 (MQ=255)
cTGATCGACCAGGATAAATGCCGTGGCTGGCGTATGTGCATCACTGGATGCCCGTACAAAAAAATc > 1:1745873/1‑66 (MQ=255)
cTGATCGACCAGGATAAATGCCGTGGCTGGCGTATGTGCATCACTGGATGCCCGTACAAAAAAATc > 1:1661322/1‑66 (MQ=255)
cTGATCGACCAGGATAAATGCCGTGGCTGGCGTATGTGCATCACTGGATGCCCGTACAAAAAAATCTAc > 1:2621855/1‑69 (MQ=255)
gATCGACCAGGATAAATGCCGTGGCTGGCGTATGTGCATCACTGGATGCCCGTACAAAAAAATCTACt > 1:2118148/1‑68 (MQ=255)
gATCGACCAGGATAAATGCCGTGGCTGGCGTATGTGCATCACTGGATGCCCGTACAAAAAAATCTACt > 1:233324/1‑68 (MQ=255)
gATCGACCAGGATAAATGCCGTGGCTGGCGTATGTGCATCACTGGATGCCCGTACAAAAAAATCTACt > 1:2415787/1‑68 (MQ=255)
cGATCAGGATAAATGCCGTGGCTGGCGTTTGTGCATTAGCTGTTGTCCGTACAAAAAAATCTACTTCaa > 1:2503906/1‑69 (MQ=255)
cGATCAGGATAAATGCCGTGGCTGGCGTTTGTGCATTAGCGGTTGTCCGTACAAAAAAATCTACTTCaa > 1:3377566/1‑69 (MQ=255)
cGATCAGGATAAATGCCGTGGCTGGCGTTTGTGCATTAGCGGTTGTCCGTACAAAAAAATCTACTTCaa > 1:2863268/1‑69 (MQ=255)
cGATCAGGATAAATGCCGTGGCTGGCGTTTGTGCATTAGCGGTTGTCCGTACAAAAAAATCTACTTCaa > 1:268636/1‑69 (MQ=255)
cGATCAGGATAAATGCCGTGGCTGGCGTTTGTGCATTAGCGGTTGTCCGTACAAAAAAATCTACTTCaa > 1:2914853/1‑69 (MQ=255)
cGATCAGGATAAATGCCGTGGCTGGCGTTTGTGCATTAGCGGTTGTCCGTACAAAAAAATCTACTTCaa > 1:296109/1‑69 (MQ=255)
cGATCAGGATAAATGCCGTGGCTGGCGTTTGTGCATTAGCGGTTGTCCGTACAAAAAAATCTACTTCaa > 1:3110189/1‑69 (MQ=255)
cGATCAGGATAAATGCCGTGGCTGGCGTTTGTGCATTAGCGGTTGTCCGTACAAAAAAATCTACTTCaa > 1:3195430/1‑69 (MQ=255)
cGATCAGGATAAATGCCGTGGCTGGCGTTTGTGCATTAGCGGTTGTCCGTACAAAAAAATCTACTTCaa > 1:3217106/1‑69 (MQ=255)
cGATCAGGATAAATGCCGTGGCTGGCGTTTGTGCATTAGCGGTTGTCCGTACAAAAAAATCTACTTCaa > 1:1615436/1‑69 (MQ=255)
cGATCAGGATAAATGCCGTGGCTGGCGTTTGTGCATTAGCGGTTGTCCGTACAAAAAAATCTACTTCaa > 1:1655519/1‑69 (MQ=255)
cGATCAGGATAAATGCCGTGGCTGGCGTTTGTGCATTAGCGGTTGTCCGTACAAAAAAATCTACTTCaa > 1:2347448/1‑69 (MQ=255)
cGATCAGGATAAATGCCGTGGCTGGCGTTTGTGCATTAGCGGTTGTCCGTACAAAAAAATCTACTTCaa > 1:3422146/1‑69 (MQ=255)
cGATCAGGATAAATGCCGTGGCTGGCGTTTGTGCATTAGCGGTTGTCCGTACAAAAAAATCTACTTCaa > 1:2257433/1‑69 (MQ=255)
cGATCAGGATAAATGCCGTGGCTGGCGTTTGTGCATTAGCGGTTGTCCGTACAAAAAAATCTACTTCaa > 1:485639/1‑69 (MQ=255)
cGATCAGGATAAATGCCGTGGCTGGCGTTTGTGCATTAGCGGTTGTCCGTACAAAAAAATCTACTTCaa > 1:2203306/1‑69 (MQ=255)
cGATCAGGATAAATGCCGTGGCTGGCGTTTGTGCATTAGCGGTTGTCCGTACAAAAAAATCTACTTCaa > 1:1346399/1‑69 (MQ=255)
cGATCAGGATAAATGCCGTGGCTGGCGTTTGTGCATTAGCGGTTGTCCGTACAAAAAAATCTACTTCaa > 1:623630/1‑69 (MQ=255)
cGATCAGGATAAATGCCGTGGCTGGCGTTTGTGCATTAGCGGTTGTCCGTACAAAAAAATCTACTTCaa > 1:1916758/1‑69 (MQ=255)
cGATCAGGATAAATGCCGTGGCTGGCGTTTGTGCATTAGCGGTTGTCCGTACAAAAAAATCTACTTCa > 1:2042869/1‑68 (MQ=255)
aGGATAAATGCCGTGGCTGGCGTATGTGCATCACTGGATGCCCGTACAAAAAAATCTACTTCAACTGGa < 1:1110335/69‑1 (MQ=255)
aGGATAAATGCCGTGGCTGGCGTATGTGCATCACTGGATGCCCGTACAAAAAAATCTACTTCAACTGGa < 1:2637181/69‑1 (MQ=255)
tggcGTATGTGCATCACCGGATGCCCGTACAAAAAAATCTACTTCAACTgg < 1:1399420/51‑1 (MQ=255)
tggcGTATGTGCATCACCGGATGCCCGTACAAAAAAATCTACTTCAACTgg < 1:1268685/51‑1 (MQ=255)
tggcGTATGTGCATCACCGGATGCCCGTACAAAAAAATCTACTTCAACTgg < 1:206763/51‑1 (MQ=255)
tggcGTATGTGCATCACCGGATGCCCGTACAAAAAAATCTACTTCAACTgg < 1:534812/51‑1 (MQ=255)
tggcGTATGTGCATCACCGGATGCCCGTACAAAAAAATCTACTTCAACTgg < 1:509049/51‑1 (MQ=255)
tggcGTATGTGCATCACCGGATGCCCGTACAAAAAAATCTACTTCAACTgg < 1:1902704/51‑1 (MQ=255)
tggcGTATGTGCATCACCGGATGCCCGTACAAAAAAATCTACTTCAACTgg < 1:1352304/51‑1 (MQ=255)
tggcGTATGTGCATCACCGGATGCCCGTACAAAAAAATCTACTTCAACTgg < 1:433195/51‑1 (MQ=255)
tggcGTATGTGCATCACCGGATGCCCGTACAAAAAAATCTACTTCAACTgg < 1:2891493/51‑1 (MQ=255)
tggcGTATGTGCATCACCGGATGCCCGTACAAAAAAATCTACTTCAACTgg < 1:2343997/51‑1 (MQ=255)
tggcGTATGTGCATCACCGGATGCCCGTACAAAAAAATCTACTTCAACTgg < 1:3262679/51‑1 (MQ=255)
tggcGTATGTGCATCACCGGATGCCCGTACAAAAAAATCTACTTCAACTgg < 1:1577879/51‑1 (MQ=255)
tggcGTATGTGCATCACCGGATGCCCGTACAAAAAAATCTACTTCAACTgg < 1:1413475/51‑1 (MQ=255)
tggcGTATGTGCATCACCGGATGCCCGTACAAAAAAATCTACTTCAACTgg < 1:1224244/51‑1 (MQ=255)
tggcGTATGTGCATCACCGGATGCCCGTACAAAAAAATCTACTTCAACTgg < 1:731635/51‑1 (MQ=255)
tggcGTATGTGCATCACCGGATGCCCGTACAAAAAAATCTACTTCAACTgg < 1:1453819/51‑1 (MQ=255)
tggcGTATGTGCATCACCGGATGCCCGTACAAAAAAATCTACTTCAACTgg < 1:1489207/51‑1 (MQ=255)
tggcGTATGTGCATCACCGGATGCCCGTACAAAAAAATCTACTTCAACTgg < 1:2914771/51‑1 (MQ=255)
tggcGTATGTGCATCAACGGATGCCCGTACAAAAAAATCTACTTCAACTgg < 1:1378344/51‑1 (MQ=255)
tgtgCATCACTGGATGCCCGTACAAAAAAATCTACTTCAACTgg > 1:2591720/1‑44 (MQ=255)
tgtgCATCACTGGATGCCCGTACAAAAAAATCTACTTCAACTgg > 1:1462942/1‑44 (MQ=255)
tgtgCATCACTGGATGCCCGTACAAAAAAATCTACTTCAACTgg < 1:2968074/44‑1 (MQ=255)
tgtgCATCACTGGATGCCCGTACAAAAAAATCTACTTCAACTgg > 1:217486/1‑44 (MQ=255)
tgCATCACTGGATGCCCGTACAAAAAAATCTACTTc < 1:2296006/36‑1 (MQ=255)
tgCATCACTGGATGCCCGTACAAAAAAATCTACTTc < 1:848541/36‑1 (MQ=255)
tgCATCACTGGATGCCCGTACAAAAAAATCTACTTc < 1:621931/36‑1 (MQ=255)
tgCATCACTGGATGCCCGTACAAAAAAATCTACTTc < 1:1332468/36‑1 (MQ=255)
tgCATCACTGGATGCCCGTACAAAAAAATCTACTTc < 1:3208100/36‑1 (MQ=255)
tgCATCACTGGATGCCCGTACAAAAAAATCTACTTc < 1:2504409/36‑1 (MQ=255)
aCTGGATGCCCGTACAAAAAAATCTACTTCAACTGGAAGAGCGGTAAGTCTGAGAAGTGCATCTTCTGc > 1:569719/1‑69 (MQ=255)
aCTGGATGCCCGTACAAAAAAATCTACTTCAACTGGAAGAGCGGTAAGTCTGAGAAGTGCATCTTCTGc > 1:3209292/1‑69 (MQ=255)
aCTGGATGCCCGTACAAAAAAATCTACTTCAACTGGAAGAGCGGTAAGTCTGAGAAGTGCATCTTCTGc > 1:2017355/1‑69 (MQ=255)
|
AGCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGATAAATGCCGTGGCTGGCGTATGTGCATCACTGGATGCCCGTACAAAAAAATCTACTTCAACTGGAAGAGCGGTAAGTCTGAGAAGTGCATCTTCTGC > minE/835890‑836023
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
