Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A2 F1 I0 R1 4577 57.4 3436498 77.7% 2670158 63.4

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 857,834 C→T 54.8% A302A (GCG→GCA)  trpC ← fused indole‑3‑glycerolphosphate synthetase and N‑(5‑phosphoribosyl)anthranilate isomerase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE857,8340CT54.8% 23.1 / 115.7 137A302A (GCG→GCA) trpCfused indole‑3‑glycerolphosphate synthetase and N‑(5‑phosphoribosyl)anthranilate isomerase
Reads supporting (aligned to +/- strand):  ref base C (24/38);  new base T (26/49);  total (50/87)
Fisher's exact test for biased strand distribution p-value = 7.22e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.88e-01

Only 100 of 152 total aligned reads displayed.

AGCTTTGTCCACCACATCGGCAATATCGTGATTGCGGAACACGCCAACATACTGCAACGGTGCCGCAGCCATCACTTCCTGCGCCTGTTCAACGTTGACGCAACGCGGTGATGTCGCAACAAAAATCAA  >  minE/857771‑857899
                                                               |                                                                 
  cTTTGTCCACCACATCGGCAATATCTTGATTGCGGAACACGCCAACATACTGCAACGGTGCCGCAGc                                                              <  1:1604625/67‑1 (MQ=255)
  cTTTGTCCACCACATCGGCAATATCGTGATTGCGGAACACGCCAACATACTGCAACGGTGCCGCAGc                                                              <  1:546709/67‑1 (MQ=255)
  cTTTGTCCACCACATCGGCAATATCGTGATTGCGGAACACGCCAACATACTGCAACGGTGCCGCAGc                                                              <  1:713457/67‑1 (MQ=255)
  cTTTGTCCACCACATCGGCAATATCGTGATTGCGGAACACGCCAACATACTGCAACGGTGCCGCAGc                                                              <  1:2188430/67‑1 (MQ=255)
  cTTTGTCCACCACATCGGCAATATCGTGATTGCGGAACACGCCAACATACTGCAACGGTGCCGCAGc                                                              <  1:804682/67‑1 (MQ=255)
  cTTTGTCCACCACATCGGCAATATCGTGATTGCGGAACACGCCAACATACTGCAACGGTGCCGCAGc                                                              <  1:931505/67‑1 (MQ=255)
   tttGTCCACCACATCGGCAATATCGTGATTGCGGAACACGCCAACATACTGCAACGGTGCCGCAGCCAt                                                           <  1:1298419/69‑1 (MQ=255)
   tttGTCCACCACATCGGCAATATCGTGATTGCGGAACACGCCAACATACTGCAACGGTGCCGCAGCCAt                                                           <  1:3070401/69‑1 (MQ=255)
   tttGTCCACCACATCGGCAATATCGTGATTGCGGAACACGCCAACATACTGCAACGGTGCCGCAGCCAt                                                           <  1:1269569/69‑1 (MQ=255)
   tttGTCCACCACATCGGCAATATCGTGATTGCGGAACACGCCAACATACTGCAACGGTGCCGCAGCCAt                                                           <  1:3282746/69‑1 (MQ=255)
   tttGTCCACCACATCGGCAATATCGTGATTGCGGAACACGCCAACATACTGCAACGGTGCCGCAGCCAt                                                           <  1:2113108/69‑1 (MQ=255)
             acaTCGGCAATATCGTGATTGCGGACCACGCCAACATACTGCAACGGTGCCGCAGCCATCACTTcc                                                    >  1:2245667/1‑66 (MQ=255)
             acaTCGGCAATATCGTGATTGCGGAACACGCCAACATACTGCAACGGTGCCGCAGCCATCACTTcc                                                    >  1:2336075/1‑66 (MQ=255)
             acaTCGGCAATATCGTGATTGCGGAACACGCCAACATACTGCAACGGTGCCGCAGCCATCACTTcc                                                    >  1:1814657/1‑66 (MQ=255)
             acaTCGGCAATATCGTGATTGCGGAACACGCCAACATACTGCAACGGTGCCGCAGCCATCACTTcc                                                    >  1:61687/1‑66 (MQ=255)
             acaTCGGCAATATCGTGATTGCGGAACACGCCAACATACTGCAACGGTGCCGCAGCCATCACTTcc                                                    >  1:3191736/1‑66 (MQ=255)
             acaTCGGCAATATCGTGATTGCGGAACACGCCAACATACTGCAACGGTGCCGCAGCCATCACTTcc                                                    >  1:2944613/1‑66 (MQ=255)
             acaTCGGCAATATCGTGATTGCGGAACACGCCAACATACTGCAACGGTGCCGCAGCCATCACTTcc                                                    >  1:2816334/1‑66 (MQ=255)
             acaTCGGCAATATCGTGATTGCGGAACACGCCAACATACTGCAACGGTGCCGCAGCCATCACTTcc                                                    >  1:776492/1‑66 (MQ=255)
             acaTCGGCAATATCGTGATTGCGGAACACGCCAACATACTGCAACGGTGCCGCAGCCATCACTTcc                                                    >  1:2676570/1‑66 (MQ=255)
             acaTCGGCAATATCGTGATTGCGGAACACGCCAACATACTGCAACGGTGACGCAGCCATCACTTcc                                                    >  1:1428927/1‑66 (MQ=255)
                  ggCAATATCGTGATTGCGGAACACGCCAACATACTGCAACGGTGCTGCAGCCATCACTTCCTGCGCCTg                                            <  1:2873141/69‑1 (MQ=255)
                  ggCAATATCGTGATTGCGGAACACGCCAACATACTGCAACGGTGCTGCAGCCATCACTTCCTGCGCCTg                                            <  1:322203/69‑1 (MQ=255)
                  ggCAATATCGTGATTGCGGAACACGCCAACATACTGCAACGGTGCTGCAGCCATCACTTCCTGCGCCTg                                            <  1:2422192/69‑1 (MQ=255)
                  ggCAATATCGTGATTGCGGAACACGCCAACATACTGCAACGGTGCTGCAGCCATCACTTCCTGCGCCTg                                            <  1:2452429/69‑1 (MQ=255)
                  ggCAATATCGTGATTGCGGAACACGCCAACATACTGCAACGGTGCTGCAGCCATCACTTCCTGCGCCTg                                            <  1:2193495/69‑1 (MQ=255)
                  ggCAATATCGTGATTGCGGAACACGCCAACATACTGCAACGGTGCTGCAGCCATCACTTCCTGCGCCTg                                            <  1:275759/69‑1 (MQ=255)
                  ggCAATATCGTGATTGCGGAACACGCCAACATACTGCAACGGTGCTGCAGCCATCACTTCCTGCGCCTg                                            <  1:1004328/69‑1 (MQ=255)
                  ggCAATATCGTGATTGCGGAACACGCCAACATACTGCAACGGTGCTGCAGCCATCACTTCCTGCGCCTg                                            <  1:3107858/69‑1 (MQ=255)
                  ggCAATATCGTGATTGCGGAACACGCCAACATACTGCAACGGTGCTGCAGCCATCACTTCCTGCGCCTg                                            <  1:2563850/69‑1 (MQ=255)
                  ggCAATATCGTGATTGCGGAACACGCCAACATACTGCAACGGTGCTGCAGCCATCACTTCCTGCGCCTg                                            <  1:2082948/69‑1 (MQ=255)
                  ggCAATATCGTGATTGCGGAACACGCCAACATACTGCAACGGTGCTGCAGCCATCACTTCCTGCGCCTg                                            <  1:1106198/69‑1 (MQ=255)
                  ggCAATATCGTGATTGCGGAACACGCCAACATACTGCAACGGTGCTGCAGCCATCACTTCCTGCGCCTg                                            <  1:3400687/69‑1 (MQ=255)
                  ggCAATATCGTGATTGCGGAACACGCCAACATACTGCAACGGTGCTGCAGCCATCACTTCCTGCGCCTg                                            <  1:116704/69‑1 (MQ=255)
                  ggCAATATCGTGATTGCGGAACACGCCAACATACTGCAACGGTGCTGCAGCCATCACTTCCTGCGCCTg                                            <  1:1856363/69‑1 (MQ=255)
                  ggCAATATCGTGATTGCGGAACACGCCAACATACTGCAACGGTGCTGCAGCCATCACTTCCTGCGCCTg                                            <  1:91251/69‑1 (MQ=255)
                  ggCAATATCGTGATTGCGGAACACGCCAACATACTGCAACGGTGCTGCAGCCATCACTTCCTGCGCCTg                                            <  1:1574255/69‑1 (MQ=255)
                  ggCAATATCGTGATTGCGGAACACGCCAACATACTGCAACGGTGCTGCAGCCATCACTTCCTGCGCCTg                                            <  1:673673/69‑1 (MQ=255)
                  ggCAATATCGTGATTGCGGAACACGCCAACATACTGCAACGGTGCTGCAGCCATCACTTCCTGCGCCTg                                            <  1:1681015/69‑1 (MQ=255)
                  ggCAATATCGTGATTGCGGAACACGCCAACATACTGCAACGGTGACGCAGCCATCACTTCCTg                                                  >  1:2823837/1‑63 (MQ=255)
                   gCAATATCGTGATTGCGGAACACGCCAACATACTGCAACGGTGCTGCAGCCATCACTTCCTGCGCCTg                                            <  1:2712615/68‑1 (MQ=255)
                        atCGTGATTGCGGAACACGCCAACATACTGCAACGGTGCCGCAGCCATCACTTCCTg                                                  >  1:1071544/1‑57 (MQ=255)
                        atCGTGATTGCGGAACACGCCAACATACTGCAACGGTGCCGCAGCCATCACTTCCTGCGCCTGTTCAAc                                      >  1:3157072/1‑69 (MQ=255)
                        atCGTGATTGCGGAACACGCCAACATACTGCAACGGTGCCGCAGCCATCACTTCCTGCGCCTGTTCAAc                                      >  1:2762139/1‑69 (MQ=255)
                        atCGTGATTGCGGAACACGCCAACATACTGCAACGGTGCCGCAGCCATCACTTCCTGCGCCTGTTCAAc                                      >  1:1548979/1‑69 (MQ=255)
                         tCGTGATTGCGGAACACGCCAACATACTGCAACGGTGCTGCAGCCATCACTTCCTGCGCCTGTTCATCg                                     <  1:2574370/69‑1 (MQ=255)
                         tCGTGATTGCGGAACACGCCAACATACTGCAACGGTGCTGCAGCCATCACTTCCTGCGCCTGTTCAACg                                     >  1:709664/1‑69 (MQ=255)
                         tCGTGATTGCGGAACACGCCAACATACTGCAACGGTGCTGCAGCCATCACTTCCTGCGCCTGTTCAACg                                     >  1:2920084/1‑69 (MQ=255)
                         tCGTGATTGCGGAACACGCCAACATACTGCAACGGTGCTGCAGCCATCACTTCCTGCGCCTGTTCAACg                                     >  1:729298/1‑69 (MQ=255)
                         tCGTGATTGCGGAACACGCCAACATACTGCAACGGTGCTGCAGCCATCACTTCCTGCGCCTGTTCAACg                                     >  1:773755/1‑69 (MQ=255)
                         tCGTGATTGCGGAACACGCCAACATACTGCAACGGTGCTGCAGCCATCACTTCCTGCGCCTGTTCAACg                                     <  1:2989097/69‑1 (MQ=255)
                         tCGTGATTGCGGAACACGCCAACATACTGCAACGGTGCTGCAGCCATCACTTCCTGCGCCTGTTCAACg                                     >  1:611366/1‑69 (MQ=255)
                         tCGTGATTGCGGAACACGCCAACATACTGCAACGGTGCTGCAGCCATCACTTCCTGCGCCTGTTCAACg                                     >  1:2722961/1‑69 (MQ=255)
                         tCGTGATTGCGGAACACGCCAACATACTGCAACGGTGCTGCAGCCATCACTTCCTGCGCCTGTTCAACg                                     <  1:3065026/69‑1 (MQ=255)
                         tCGTGATTGCGGAACACGCCAACATACTGCAACGGTGCTGCAGCCATCACTTCCTGCGCCTGTTCAACg                                     >  1:2511631/1‑69 (MQ=255)
                         tCGTGATTGCGGAACACGCCAACATACTGCAACGGTGCTGCAGCCATCACTTCCTGCGCCTGTTCAACg                                     >  1:2511046/1‑69 (MQ=255)
                         tCGTGATTGCGGAACACGCCAACATACTGCAACGGTGCTGCAGCCATCACTTCCTGCGCCTGTTCAACg                                     >  1:1842178/1‑69 (MQ=255)
                         tCGTGATTGCGGAACACGCCAACATACTGCAACGGTGCTGCAGCCATCACTTCCTGCGCCTGTTCAACg                                     <  1:1765363/69‑1 (MQ=255)
                         tCGTGATTGCGGAACACGCCAACATACTGCAACGGTGCTGCAGCCATCACTTCCTGCGCCTGTTCAACg                                     >  1:2356930/1‑69 (MQ=255)
                         tCGTGATTGCGGAACACGCCAACATACTGCAACGGTGCTGCAGCCATCACTTCCTGCGCCTGTTCAACg                                     >  1:230152/1‑69 (MQ=255)
                         tCGTGATTGCGGAACACGCCAACATACTGCAACGGTGCTGCAGCCATCACTTCCTGCGCCTGTTCAACg                                     >  1:2031812/1‑69 (MQ=255)
                         tCGTGATTGCGGAACACGCCAACATACTGCAACGGTGCTGCAGCCATCACTTCCTGCGCCTGTTCAACg                                     >  1:1633744/1‑69 (MQ=255)
                         tCGTGATTGCGGAACACGCCAACATACTGCAACGGTGCTGCAGCCATCACTTCCTGCGCCTGTTCAACg                                     <  1:2114667/69‑1 (MQ=255)
                         tCGTGATTGCGGAACACGCCAACATACTGCAACGGTGCTGCAGCCATCACTTCCTGCGCCTGTTCAACg                                     >  1:2155043/1‑69 (MQ=255)
                         tCGTGATTGCGGAACACGCCAACATACTGCAACGGTGCTGCAGCCATCACTTCCTGCGCCTGTTCAACg                                     >  1:2177000/1‑69 (MQ=255)
                         tCGTGATTGCGGAACACGCCAACATACTGCAACGGTGCTGCAGCCATCACTTCCTGCGCCTGTTCAACg                                     >  1:143454/1‑69 (MQ=255)
                         tCGTGATTGCGGAACACGCCAACATACTGCAACGGTGCTGCAGCCATCACTTCCTGCGCCTGTTCAACg                                     <  1:146420/69‑1 (MQ=255)
                         tCGTGATTGCGGAACACGCCAACATACTGCAACGGTGCTGCAGCCATCACTTCCTGCGCCTGTTCAACg                                     <  1:1621447/69‑1 (MQ=255)
                         tCGTGATTGCGGAACACGCCAACATACTGCAACGGTGCCGCAGCCATCACTTCCTGCGCCTGTTCAACg                                     >  1:456201/1‑69 (MQ=255)
                         tCGTGATTGCGGAACACGCCAACATACTGCAACGCTGCTGCAGCCATCACTTCCTGCGCCTGTTCAACg                                     >  1:2664568/1‑69 (MQ=255)
                          cGTGATTGCGGAACACGCCAACATACTGCAACGGTGCTGCAGCCATCACTTCCTGCGCCTGTTCAACg                                     <  1:3140070/68‑1 (MQ=255)
                          cGTGATTGCGGAACACGCCAACATACTGCAACGGTGCTGCAGCCATCACTTCCTGCGCCTGTTCAACg                                     <  1:20048/68‑1 (MQ=255)
                          cGTGATTGCGGAACACGCCAACATACTGCAACGGTGCTGCAGCCATCACTTCCTGCGCCTGTTCAACg                                     <  1:2404715/68‑1 (MQ=255)
                          cGTGATTGCGGAACACGCCAACATAATGCAACGGTGCTGCAGCCATCACTTCCTGCGCCTGTTCAACg                                     <  1:1675715/68‑1 (MQ=255)
                           gTGATTGCGGAACACGCCAACATACTGCAACGGTGCTGCAGCCATCACTTCCTGCGCCTGTTCAACg                                     <  1:654904/67‑1 (MQ=255)
                            tGATTGCGGAACACGCCAACATACTGCAACGGTGCCGCAGCCATCACTTCCTGCGCCTGTTCAACGTTg                                  >  1:97858/1‑69 (MQ=255)
                                tGCGGAACACGCCAACATACTGCAACGGTGCCGCAGc                                                              <  1:3094118/37‑1 (MQ=255)
                                     aaCACGCCAACATACTGCAACGGTGCCGCAGCCATCACTTCCTGCGCCTGTTCAACGTTGACGCAAcgc                         <  1:2225877/69‑1 (MQ=255)
                                        acGCCAACATACTGCAACGGTGCCGCAGCCATCACTTCCTGCGCCTGTTCAACGTTGACGCAACGCGGt                      <  1:870315/69‑1 (MQ=255)
                                        acGCCAACATACTGCAACGGTGCCGCAGCCATCACTTCCTGCGCCTGTTCAACGTTGACGCAACGCGGt                      <  1:289468/69‑1 (MQ=255)
                                        acGCCAACATACTGCAACGGTGCCGCAGCCATCACTTCCTGCGCCTGTTCAACGTTGACGCAACGCGGt                      <  1:2292488/69‑1 (MQ=255)
                                        acGCCAACATACTGCAACGGTGCCGCAGCCATCACTTCCTGCGCCTGTTCAACGTTGACGCAACGCGGt                      <  1:72491/69‑1 (MQ=255)
                                        acGCCAACATACTGCAACGGTGCCGCAGCCATCACTTCCTGCGCCTGTTCAACGTTGACGCAACGCGGt                      <  1:1929653/69‑1 (MQ=255)
                                                aTACTGCAACGGTGCCGCAGCCATCACTTCCTGCGCCTGTTCAACGTTGACGCAACGCGGTGATGTCGc              <  1:1286722/69‑1 (MQ=255)
                                                aTACTGCAACGGTGCCGCAGCCATCACTTCCTGCGCCTGTTCAACGTTGACGCAACGCGGTGATGTCGc              <  1:2590511/69‑1 (MQ=255)
                                                aTACTGCAACGGTGCCGCAGCCATCACTTCCTGCGCCTGTTCAACGTTGACGCAACGCGGTGATGTCGc              <  1:3223841/69‑1 (MQ=255)
                                                aTACTGCAACGGTGCCGCAGCCATCACTTCCTGCGCCTGTTCAACGTTGACGCAACGCGGTGATGTCGc              <  1:295697/69‑1 (MQ=255)
                                                   cTGCAACGGTGCCGCAGCCATCACTTCCTGCGCCTGTTCAACGTTGACGCAACGCGGTGATGTCGcaa            >  1:2452450/1‑68 (MQ=255)
                                                          ggTGCCGCAGCCATCACTTCCTGCGCCTGTTCAACGTTGACGCAACGCGGTGa                    <  1:1781013/53‑1 (MQ=255)
                                                            tgctgcATCCATCACTTCCTGCGCCTGTTCAACGTTGACGCAACGCGGTGATGTCGCAACAAAAATCaa  >  1:2240213/1‑69 (MQ=255)
                                                            tgctgcAGCCATCACTTCCTGCGCCTGTTCAACGTTGACGCAACGCGGTGATGTCGCAACAAAAATCaa  >  1:3401765/1‑69 (MQ=255)
                                                            tgctgcAGCCATCACTTCCTGCGCCTGTTCAACGTTGACGCAACGCGGTGATGTCGCAACAAAAATCaa  >  1:2001170/1‑69 (MQ=255)
                                                            tgctgcAGCCATCACTTCCTGCGCCTGTTCAACGTTGACGCAACGCGGTGATGTCGCAACAAAAATCaa  >  1:3338632/1‑69 (MQ=255)
                                                            tgctgcAGCCATCACTTCCTGCGCCTGTTCAACGTTGACGCAACGCGGTGATGTCGCAACAAAAATCaa  >  1:1715015/1‑69 (MQ=255)
                                                            tgctgcAGCCATCACTTCCTGCGCCTGTTCAACGTTGACGCAACGCGGTGATGTCGCAACAAAAATCaa  >  1:2023894/1‑69 (MQ=255)
                                                            tgctgcAGCCATCACTTCCTGCGCCTGTTCAACGTTGACGCAACGCGGTGATGTCGCAACAAAAATCaa  >  1:2084567/1‑69 (MQ=255)
                                                            tgctgcAGCCATCACTTCCTGCGCCTGTTCAACGTTGACGCAACGCGGTGATGTCGCAACAAAAATCaa  >  1:3002372/1‑69 (MQ=255)
                                                            tgctgcAGCCATCACTTCCTGCGCCTGTTCAACGTTGACGCAACGCGGTGATGTCGCAACAAAAATCaa  >  1:2218289/1‑69 (MQ=255)
                                                            tgctgcAGCCATCACTTCCTGCGCCTGTTCAACGTTGACGCAACGCGGTGATGTCGCAACAAAAATCaa  >  1:2923645/1‑69 (MQ=255)
                                                            tgctgcAGCCATCACTTCCTGCGCCTGTTCAACGTTGACGCAACGCGGTGATGTCGCAACAAAAATCa   >  1:1334826/1‑68 (MQ=255)
                                                               |                                                                 
AGCTTTGTCCACCACATCGGCAATATCGTGATTGCGGAACACGCCAACATACTGCAACGGTGCCGCAGCCATCACTTCCTGCGCCTGTTCAACGTTGACGCAACGCGGTGATGTCGCAACAAAAATCAA  >  minE/857771‑857899

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A