Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F1 I0 R1
|
4577 |
57.4 |
3436498 |
77.7% |
2670158 |
63.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
860,344 |
A→C |
57.6% |
T519T (ACT→ACG) |
trpE ← |
component I of anthranilate synthase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 860,344 | 0 | A | C | 57.6%
| 34.8
/ 60.3
| 66 | T519T (ACT→ACG) | trpE | component I of anthranilate synthase |
| Reads supporting (aligned to +/- strand): ref base A (9/19); new base C (18/20); total (27/39) |
| Fisher's exact test for biased strand distribution p-value = 3.11e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.15e-01 |
TGATCTGCCAGGTTGTACGTAAAAGAGTCGATATTATCGAGCAGCAGAATGTCAGCCATCAGAAAGTCTCCTGTGCATGATGCGCGGTGGCAATAGCGCGCAGTACAGCGCGGGCTTTGTTAC > minE/860280‑860402
|
tGATCTGCCAGGTTGTACGTAAAAGAGTCGATATTATCGAGCAGCAGAATGTCAGCCATCAGAAAGtct < 1:2829279/69‑1 (MQ=255)
tGATCTGCCAGGTTGTACGTAAAAGAGTCGATATTATCGAGCAGCAGAATGTCAGCCATCAGAAAGtct < 1:3202866/69‑1 (MQ=255)
tGATCTGCCAGGTTGTACGTAAAAGAGTCGATATTATCGAGCAGCAGAATGTCAGCCATCAGAAAGtct < 1:2070492/69‑1 (MQ=255)
tGATCTGCCAGGTTGTACGTAAAAGAGTCGATATTATCGAGCAGCAGAATGTCAGCCATCAGAAAGtct < 1:523122/69‑1 (MQ=255)
tGATCTGCCAGGTTGTACGTAAAAGAGTCGATATTATCGAGCAGCAGAATGTCAGCCATCAGAAAGtct < 1:1955262/69‑1 (MQ=255)
tGATCTGCCAGGTTGTACGTAAAAGAGTCGATATTATCGAGCAGCAGAATGTCAGCCATCAGAAAGtct < 1:1273756/69‑1 (MQ=255)
gTTGTACGTAAAAGAGTCGATATTATCGAGCAGCAGAATGTCAGCCATCAGAAAGTCTCCTGTGCatga > 1:3213245/1‑69 (MQ=255)
gTTGTACGTAAAAGAGTCGATATTATCGAGCAGCAGAATGTCAGCCATCAGAAAGTCTCCTGTGCatga > 1:272295/1‑69 (MQ=255)
gTTGTACGTAAAAGAGTCGATATTATCGAGCAGCAGAATGTCAGCCATCAGAAAGTCTCCTGTGCatga > 1:2024916/1‑69 (MQ=255)
tGTACGTAAAAGAGTCGATATTATCGAGCAGCAGAATGTCAGCCATTAGAACGTCTCCTGTGc > 1:1729004/1‑63 (MQ=255)
tGTACGTAAAAGAGTCGATATTATCGAGCAGCAGAATGTCAGCCATTAGAACGTCTCCTGTGCa > 1:2498542/1‑64 (MQ=255)
tGTACGTAAAAGAGTCGATATTATCGAGCAGCAGAATGTCAGCCATTAGAACGTCTCCTGTGCa > 1:2347816/1‑64 (MQ=255)
tGTACGTAAAAGAGTCGATATTATCGAGCAGCAGAATGTCAGCCATTAGAACGTCTCCTGTGCa > 1:2123767/1‑64 (MQ=255)
tGTACGTAAAAGAGTCGATATTATCGAGCAGCAGAATGTCAGCCATTAGAACGTCTCCTGTGCa > 1:2777144/1‑64 (MQ=255)
tGTACGTAAAAGAGTCGATATTATCGAGCAGCAGAATGTCAGCCATTAGAACGTCTCCTGTGCa > 1:2262597/1‑64 (MQ=255)
tGTACGTAAAAGAGTCGATATTATCGAGCAGCAGAATGTCAGCCATTAGAACGTCTCCTGTGCa > 1:1750978/1‑64 (MQ=255)
tGTACGTAAAAGAGTCGATATTATCGAGCAGCAGAATGTCAGCCATTAGAACGTCTCCTGTGCa > 1:1577276/1‑64 (MQ=255)
tGTACGTAAAAGAGTCGATATTATCGAGCAGCAGAATGTCAGCCATTAGAACGTCTCCTGTGCa > 1:1682718/1‑64 (MQ=255)
tGTACGTAAAAGAGTCGATATTATCGAGCAGCAGAATGTCAGCCATTAGAACGTCTCCTGTGCa > 1:161617/1‑64 (MQ=255)
tGTACGTAAAAGAGTCGATATTATCGAGCAGCAGAATGTCAGCCATTAGAACGTCTCCTGTGCa > 1:3113416/1‑64 (MQ=255)
tGTACGTAAAAGAGTCGATATTATCGAGCAGCAGAATGTCAGCCATTAGAACGTCTCCTGTGCa > 1:1274510/1‑64 (MQ=255)
tGTACGTAAAAGAGTCGATATTATCGAGCAGCAGAATGTCAGCCATTAGAACGTCTCCTGTGCa > 1:379127/1‑64 (MQ=255)
tGTACGTAAAAGAGTCGATATTATCGAGCAGCAGAATGTCAGCCATTAGAACGTCTCCTGTGCa > 1:1216900/1‑64 (MQ=255)
tGTACGTAAAAGAGTCGATATTATCGAGCAGCAGAATGTCAGCCATTAGAACGTCTCCTGTGCa > 1:712608/1‑64 (MQ=255)
tGTACGTAAAAGAGTCGATATTATCGAGCAGCAGAATGTCAGCCATTAGAACGTCTCCTGTGCa > 1:760115/1‑64 (MQ=255)
tGTACGTAAAAGAGTCGATATTATCGAGCAGCAGAATGTCAGCCATTAGAACGTCTCCTGAGCa > 1:498992/1‑64 (MQ=255)
tGTACGTAAAAGAGTCGATATTATCGAGCAGCAGAATGTCAGCCATTAGAACGTCCCCTGTGCa > 1:115433/1‑64 (MQ=255)
gTACGTAAAAGAGTCGATATTATCGAGCAGCAGAATGTCAGCCATCAGAAAGTCTCCTGTGCatgatg > 1:760473/1‑68 (MQ=255)
gTACGTAAAAGAGTCGATATTATCGAGCAGCAGAATGTCAGCCATCAGAAAGTCTCCTGTGCATGATgc > 1:808009/1‑69 (MQ=255)
cGTAAAAGAGTCGATATTATCGAGCAGCAGAATGTCAGCCATCAGAAAGTCTCCTGTGCATGATGcgcg > 1:1058352/1‑69 (MQ=255)
cGTAAAAGAGTCGATATTATCGAGCAGCAGAATGTCAGCCATCAGAAAGTCTCCTGTGCATGATGcgcg > 1:597961/1‑69 (MQ=255)
cGTAAAAGAGTCGATATTATCGAGCAGCAGAATGTCAGCCATCAGAAAGTCTCCTGTGCATGATGcgcg > 1:1448499/1‑69 (MQ=255)
gTAAAAGAGTCGATATTATCGAGCAGCAGAATGTCAGCCATCAGAAAGTCTCCTGTGCATGATgcgc < 1:797916/67‑1 (MQ=255)
agagTCGATATTATCGAGCAGCAGAATGTCAGCCATCAGAAAGTCTCCTGTGCATGATGCGCGGTGGc < 1:3211059/68‑1 (MQ=255)
agagTCGATATTATCGAGCAGCAGAATGTCAGCCATCAGAAAGTCTCCTGTGCATGATGCGCGGTGGc < 1:1138000/68‑1 (MQ=255)
agagTCGATATTATCGAGCAGCAGAATGTCAGCCATCAGAAAGTCTCCTGTGCATGATGCGCGGTGGc < 1:1505209/68‑1 (MQ=255)
gagTCGATATTATCGAGCAGCAGAATGTCAGCCATCAGAAAGTCTCCTGTGCATGATGCGCGGTGGCaa < 1:1117111/69‑1 (MQ=255)
tCGATATTATCGAGCAGCAGAATGTCAGCCATCAGAAAGtct < 1:1510488/42‑1 (MQ=255)
gAATGTCAGCCATTAGAACGTCTCCTGTGCATGATGCGCGGTGGCAATAGCGCGCTGTACAGCGCGGGc < 1:1261639/69‑1 (MQ=255)
gAATGTCAGCCATTAGAACGTCTCCTGTGCATGATGCGCGGTGGCAATAGCGCGCAGTACAGCGCGGGc < 1:2079218/69‑1 (MQ=255)
gAATGTCAGCCATTAGAACGTCTCCTGTGCATGATGCGCGGTGGCAATAGCGCGCAGTACAGCGCGGGc < 1:2708357/69‑1 (MQ=255)
gAATGTCAGCCATTAGAACGTCTCCTGTGCATGATGCGCGGTGGCAATAGCGCGCAGTACAGCGCGGGc < 1:23537/69‑1 (MQ=255)
gAATGTCAGCCATTAGAACGTCTCCTGTGCATGATGCGCGGTGGCAATAGCGCGCAGTACAGCGCGGGc < 1:78283/69‑1 (MQ=255)
gAATGTCAGCCATTAGAACGTCTCCTGTGCATGATGCGCGGTGGCAATAGCGCGCAGTACAGCGCGGGc < 1:1095178/69‑1 (MQ=255)
gAATGTCAGCCATTAGAACGTCTCCTGTGCATGATGCGCGGTGGCAATAGCGCGCAGTACAGCGCGGGc < 1:2447646/69‑1 (MQ=255)
gAATGTCAGCCATTAGAACGTCTCCTGTGCATGATGCGCGGTGGCAATAGCGCGCAGTACAGCGCGGGc < 1:688358/69‑1 (MQ=255)
gAATGTCAGCCATTAGAACGTCTCCTGTGCATGATGCGCGGTGGCAATAGCGCGCAGTACAGCGCGGGc < 1:2027311/69‑1 (MQ=255)
gAATGTCAGCCATTAGAACGTCTCCTGTGCATGATGCGCGGTGGCAATAGCGCGCAGTACAGCGCGGGc < 1:534621/69‑1 (MQ=255)
gAATGTCAGCCATTAGAACGTCTCCTGTGCATGATGCGCGGTGGCAATAGCGCGCAGTACAGCGCGGGc < 1:118840/69‑1 (MQ=255)
gAATGTCAGCCATTAGAACGTCTCCTGTGCATGATGCGCGGTGGCAATAGCGCGCAGTACAGCGCGGGc < 1:470647/69‑1 (MQ=255)
gAATGTCAGCCATTAGAACGTCTCCTGTGCATGATGCGCGGTGGCAATAGCGCGCAGTACAGCGCGGGc < 1:328834/69‑1 (MQ=255)
gAATGTCAGCCATTAGAACGTCTCCTGTGCATGATGCGCGGTGGCAATAGCGCGCAGTACAGCGCGGGc < 1:2625131/69‑1 (MQ=255)
gAATGTCAGCCATTAGAACGTCTCCTGTGCATGATGCGCGGTGGCAATAGCGCGCAGTACAGCGCGGGc < 1:2251513/69‑1 (MQ=255)
gAATGTCAGCCATTAGAACGTCTCCTGTGCATGATGCGCGGTGGCAATAGCGCGCAGTACAGCGCGGGc < 1:3003568/69‑1 (MQ=255)
gAATGTCAGCCATTAGAACGTCTCCTGTGCATGATGCGCGGTGGCAATAGCGCGCAGTACAGCGCGGGc < 1:1915957/69‑1 (MQ=255)
gAATGTCAGCCATTAGAACGTCTCCTGTGCATGATGCGCGGTGGCAATAGCGCGCAGTACAGCGCGGGc < 1:2769890/69‑1 (MQ=255)
aaTGTCAGCCATTAGAACGTCTCCTGTGCATGATGCGCGGTGGCAATAGCGCGCAGTACAGCGCGGGc < 1:1201076/68‑1 (MQ=255)
aaTGTCAGCCATTAGAACGTCTCCTGTGCATGATGCGCGGTGGCAATAGCGCGCAGTACAGCGCGGGc < 1:3061788/68‑1 (MQ=255)
tCAGCCATCAGAAAGTCTCCTGTGCATGATGCGCGGTGGCAATAGCGCGCAGTACAGCGCGGGCTTTGt < 1:354856/69‑1 (MQ=255)
tCAGCCATCAGAAAGTCTCCTGTGCATGATGCGCGGTGGCAATAGCGCGCAGTACAGCGCGGGCTTTGt < 1:2665921/69‑1 (MQ=255)
tCAGCCATCAGAAAGTCTCCTGTGCATGATGCGCGGTGGCAATAGCGCGCAGTACAGCGCGGGCTTTGt < 1:571651/69‑1 (MQ=255)
tCAGCCATCAGAAAGTCTCCTGTGCATGATGCGCGGTGGCAATAGCGCGCAGTACAGCGCGGGCTTTGt < 1:590950/69‑1 (MQ=255)
tCAGCCATCAGAAAGTCTCCTGTGCATGATGCGCGGTGGCAATAGCGCGCAGTACAGCGCGGGCTTTGt < 1:3162311/69‑1 (MQ=255)
tCAGCCATCAGAAAGTCTCCTGTGCATGATGCGCGGTGGCAATAGCGCGCAGTACAGCGCGGGCTTTGt < 1:2902089/69‑1 (MQ=255)
tCAGCCATCAGAAAGTCTCCTGTGCATGATGCGCGGTGGCAATAGCGCGCAGTACAGCGCGGGCTTTGt < 1:1935637/69‑1 (MQ=255)
gCCATCAGAAAGTCTCCTGTGCATGATGCGCGGTGGCAATAGCGCGCAGTACAGCGCGGGCTTTGTTAc > 1:1885557/1‑69 (MQ=255)
|
TGATCTGCCAGGTTGTACGTAAAAGAGTCGATATTATCGAGCAGCAGAATGTCAGCCATCAGAAAGTCTCCTGTGCATGATGCGCGGTGGCAATAGCGCGCAGTACAGCGCGGGCTTTGTTAC > minE/860280‑860402
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
