Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F1 I0 R1
|
4577 |
57.4 |
3436498 |
77.7% |
2670158 |
63.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
869,449 |
T→A |
33.3% |
T58T (ACA→ACT) |
yciN ← |
hypothetical protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 869,449 | 0 | T | A | 33.3%
| 88.2
/ 70.5
| 93 | T58T (ACA→ACT) | yciN | hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base T (27/35); new base A (15/16); total (42/51) |
| Fisher's exact test for biased strand distribution p-value = 6.66e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.72e-01 |
AGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACATATTAAACACCGCGGTGCTTTTGGCTGTTGGCAATCCTTGTTCATCTAAAAAGTAGTCGCCGGTAAATACCAGCACGC > minE/869381‑869501
|
atatgatATTTTTCTTAAAGCACATGGGCCAGGTGTTTGAACATATTAAACACCGCGGTGCTTTTGGCt < 1:2609807/67‑1 (MQ=255)
aGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAATATATTAAACACCGCGGTGCTTTTGGCt < 1:1600314/69‑1 (MQ=255)
aGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACATATTAAACACCGCGGTGCTTTTGGCt < 1:2665216/69‑1 (MQ=255)
gtgatATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACATATTAAACACCGCGGTGCTTTTGGCAGt < 1:2281396/68‑1 (MQ=255)
gtgatATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACATATTAAACACCGCGGTGCTTTTGGCAGt < 1:2421942/68‑1 (MQ=255)
gtgatATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACATATTAAACACCGCGGTGCTTTTGGCAGt < 1:1368089/68‑1 (MQ=255)
gtgatATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACATATTAAACACCGCGGTGCTTTTGGCAGt < 1:135173/68‑1 (MQ=255)
gtgatATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACATATTAAACACCGCGGTGCTTTTGGCAGt < 1:2943197/68‑1 (MQ=255)
gtgatATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACATATTAAACACCGCGGTGCTTTTGGCAGt < 1:3151016/68‑1 (MQ=255)
gtgatATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACATATTAAACACCGCGGTGCTTTTGGCAGt < 1:2170479/68‑1 (MQ=255)
gtgatATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACATATTAAACACCGCGGTGCTTTTGGCAGt < 1:3420861/68‑1 (MQ=255)
gtgatATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACATATTAAACACCGCGGTGCTTTTGGCAGt < 1:440171/68‑1 (MQ=255)
gtgatATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACATATTAAACACCGCGGTGCTTTTGGCAGt < 1:456493/68‑1 (MQ=255)
gtgatATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACATATTAAACACCGCGGTGCTTTTGGCAGt < 1:977200/68‑1 (MQ=255)
gtgatATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACATATTAAACACCGCGGTGCTTTTGGCAGt < 1:2153259/68‑1 (MQ=255)
gtgatATTTTTCTAAAAGCACATGGGCCAGGTGTTTGAACATATTAAACACCGCGGTGCTTTTGGCAGt < 1:1577174/68‑1 (MQ=255)
gtgatATTTATCTGAAAGCACATGGGCCAGGTGTTTGAACATATTAAACACCGCGGTGCTTTTGGCAGt < 1:80878/68‑1 (MQ=255)
tgatATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACATATTAAACACCGCGGTGCTTTTGGCAGt < 1:1777644/68‑1 (MQ=255)
gatATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACATATTAAACACCGCGGTGCTTTTGGCAGt < 1:1663102/67‑1 (MQ=255)
gCCAGGTGTTTGAACATATTAAACACCGCGGTGCTTTTGGCt < 1:2942652/42‑1 (MQ=255)
gtgtTTGAACATATTAAACACCGCGGTGCTTTTGGCTGTTGGCAATCCTTGTTCATCTAAAAAGTAGTc > 1:1043630/1‑69 (MQ=255)
tgtTTGAACATATTAAACACCGCGGTGCTTTTGGCTGTTGGCAATCCTTGTTCATCTAAAAAGTAGTCg > 1:251224/1‑69 (MQ=255)
tgtTTGAACATATTAAACACCGCGGTGCTTTTGGCTGTTGGCAATCCTTGTTCATCTAAAAAGTAGTCg > 1:2956517/1‑69 (MQ=255)
tgtTTGAACATATTAAACACCGCGGTGCTTTTGGCTGTTGGCAATCCTTGTTCATCTAAAAAGTAGTCg > 1:3287090/1‑69 (MQ=255)
tgtTTGAACATATTAAACACCGCGGTGCTTTTGGCTGTTGGCAATCCTTGTTCATCTAAAAAGTAGTCg > 1:2081097/1‑69 (MQ=255)
tgtTTGAACATATTAAACACCGCGGTGCTTTTGGCTGTTGGCAATCCTTGTTCATCTAAAAAGTAGTCg > 1:1078983/1‑69 (MQ=255)
tgtTTGAACATATTAAACACCGCGGTGCTTTTGGCTGTTGGCAATCCTTGTTCATCTAAAAAGTAGTCg > 1:1304604/1‑69 (MQ=255)
tGAACATATTAAACACCGCGGTGCTTTTGGCTGTTGGCAATCCTTGTTCATCTaaaaatt < 1:1724163/60‑3 (MQ=255)
tGAACATATTAAACACCGCGGTGCTTTTGGCTGTTGGCAATCCTTGTTCATCTa < 1:2695767/54‑1 (MQ=255)
tGAACATATTAAACACCGCGGTGCTTTTGGCTGTTGGCAATCCTTGTTCATCTa > 1:3431370/1‑54 (MQ=255)
tGAACATATTAAACACCGCGGTGCTTTTGGCTGTTGGCAATCCTTGTTCATCTa > 1:2486252/1‑54 (MQ=255)
tGAACATATTAAACACCGCGGTGCTTTTGGCTGTTGGCAATCCTTGTTCATCTa < 1:2792024/54‑1 (MQ=255)
tGAACATATTAAACACCGCGGTGCTTTTGGCTGTTGGCAATCCTTGTTCATCTa < 1:2346674/54‑1 (MQ=255)
tGAACATATTAAACACCGCGGTGCTTTTGGCTGTTGGCAATCCTTGTTCATCTa > 1:3380750/1‑54 (MQ=255)
tGAACATATTAAACACCGCGGTGCTTTTGGCTGTTGGCAATCCTTGTTCATCTa > 1:527434/1‑54 (MQ=255)
tGAACATATTAAACACCGCGGTGCTTTTGGCTGTTGGCAATCCTTGTTCATCTa < 1:1996800/54‑1 (MQ=255)
tGAACATATTAAACACCGCGGTGCTTTTGGCTGTTGGCAATCCTTGTTCATCTa > 1:1937560/1‑54 (MQ=255)
tGAACATATTAAACACCGCGGTGCTTTTGGCTGTTGGCAATCCTTGTTCATCTa < 1:1216259/54‑1 (MQ=255)
tGAACATATTAAACACCGCGGTGCTTTTGGCTGTTGGCAATCCTTGTTCATCTa > 1:3001904/1‑54 (MQ=255)
tGAACATATTAAACACCGCGGTGCTTTTGGCTGTTGGCAATCCTTGTTCATCTa > 1:1834157/1‑54 (MQ=255)
tGAACATATTAAACACCGCGGTGCTTTTGGCTGTTGGCAATCCTTGTTCATCTAAAAagt < 1:2019434/60‑1 (MQ=255)
tGAACATATTAAACACCGCGGTGCTTTTGGCTGTTGGCAATCCTTGTTCATCTAAAAagt < 1:2772463/60‑1 (MQ=255)
tGAACATATTAAACACCGCGGTGCTTTTGGCTGTTGGCAATCCTTGTTCATCTAAAAagt < 1:1763922/60‑1 (MQ=255)
tGAACATATTAAACACCGCGGTGCTTTTGGCTGTTGGCAATCCTTGTTCATCTAAAAagt < 1:61402/60‑1 (MQ=255)
tGAACATATTAAACACCGCGGTGCTTTTGGCTGTTGGCAATCCTTGTTCATCTAAAAagt < 1:525185/60‑1 (MQ=255)
tGAACATATTAAACACCGCGGTGCTTTTGGCTGTTGGCAATCCTTGTTCATCTAAAAagt < 1:2533742/60‑1 (MQ=255)
tGAACATATTAAACACCGCGGTGCTTTTGGCTGTTGGCAATCCTTGTTCATCTAAAAagt < 1:2219155/60‑1 (MQ=255)
tGAACATATTAAACACCGCGGTGCTTTTGGCTGTTGGCAATCCTTGTTCATCTAAAAagt < 1:1436396/60‑1 (MQ=255)
tGAACATATTAAACACCGCGGTGCTTTTGGCTGTTGGCAATCCTTGTTCATCTAAAAagt < 1:2784841/60‑1 (MQ=255)
tGAACATATTAAACACCGCGGTGCTTTTGGCTGTTGGCAATCCTTGTTCATCTAAAAAGTAGTCGCCg > 1:1904153/1‑68 (MQ=255)
tGAACATATTAAACACCGCGGTGCTTTTGGCTGTTGGCAATCCTTGTTCATCTAAAAAGTAGTCGCCg > 1:3263192/1‑68 (MQ=255)
tGAACATATTAAACACCGCGGTGCTTTTGGCAGTTTGTAATCCTTGTTCATCTAAAAagta > 1:2985217/1‑61 (MQ=255)
tGAACATATTAAACACCGCGGTGCTTTTGGCAGTTGGTAATCCTTGTTCATCTAAAAagta > 1:3118441/1‑61 (MQ=255)
tGAACATATTAAACACCGCGGTGCTTTTGGCAGTTGGTAATCCTTGTTCATCTAAAAagta > 1:2935950/1‑61 (MQ=255)
tGAACATATTAAACACCGCGGTGCTTTTGGCAGTTGGTAATCCTTGTTCATCTAAAAagta > 1:1310409/1‑61 (MQ=255)
tGAACATATTAAACACCGCGGTGCTTTTGGCAGTTGGTAATCCTTGTTCATCTAAAAagta > 1:1270133/1‑61 (MQ=255)
tGAACATATTAAACACCGCGGTGCTTTTGGCAGTTGGTAATCCTTGTTCATCTAAAAagta > 1:3191846/1‑61 (MQ=255)
tGAACATATTAAACACCGCGGTGCTTTTGGCAGTTGGTAATCCTTGTTCATCTAAAAagta > 1:353891/1‑61 (MQ=255)
tGAACATATTAAACACCGCGGTGCTTTTGGCAGTTGGTAATCCTTGTTCATCTAAAAagta > 1:1554745/1‑61 (MQ=255)
tGAACATATTAAACACCGCGGTGCTTTTGGCAGTTGGTAATCCTTGTTCATCTAAAAagta > 1:124759/1‑61 (MQ=255)
tGAACATATTAAACACCGCGGTGCTTTTGGCAGTTGGTAATCCTTGTTCATCTAAAAagta > 1:1242459/1‑61 (MQ=255)
tGAACATATTAAACACCGCGGTGCTTTTGGCAGTTGGTAATCCTTGTTCATCTAAAAagta > 1:2187092/1‑61 (MQ=255)
tGAACATATTAAACACCGCGGTGCTTTTGGCAGTTGGTAATCCTTGTTCATCTAAAAagta > 1:2097284/1‑61 (MQ=255)
tGAACATATTAAACACCGCGGTGCTTTTGGCAGTTGGTAATCCTTGTTCATCTAAAAagta > 1:2071971/1‑61 (MQ=255)
tGAACATATTAAACACCGCGGTGCTTTTGGCAGTTGGTAATCCTTGTTCATCTAAAAagta > 1:720381/1‑61 (MQ=255)
tGAACATATTAAACACCGCGGTGCTTTTGGCAGTTGGTAATCCTTGTTCATCTAAAAagta > 1:1842757/1‑61 (MQ=255)
tGAACATATTAAACACCGAGGTGCTTTTGGCTGTTGGCAATCCTTGTTCATCTa > 1:1264201/1‑54 (MQ=255)
aCATATTAAACACCGCGGTGCTTTTGGCTGTTGGCAATCCTTGTTCATCTAAAAAGTAGTCTCCGGTa < 1:3272/68‑1 (MQ=255)
aCATATTAAACACCGCGGTGCTTTTGGCTGTTGGCAATCCTTGTTCATCTAAAAAGTAGTCGCCGGTa < 1:3389822/68‑1 (MQ=255)
aCATATTAAACACCGCGGTGCTTTTGGCTGTTGGCAATCCTTGTTCATCTAAAAAGTAGTCGCCGGTa < 1:574899/68‑1 (MQ=255)
aCATATTAAACACCGCGGTGCTTTTGGCTGTTGGCAATCCTTGTTCATCTAAAAAGTAGTCGCCGGTa < 1:63669/68‑1 (MQ=255)
aCATATTAAACACCGCGGTGCTTTTGGCTGTTGGCAATCCTTGTTCATCTAAAAAGTAGTCGCCGGTa < 1:3264472/68‑1 (MQ=255)
aCATATTAAACACCGCGGTGCTTTTGGCTGTTGGCAATCCTTGTTCATCTAAAAAGTAGTCGCCGGTa < 1:1796072/68‑1 (MQ=255)
aCATATTAAACACCGCGGTGCTTTTGGCTGTTGGCAATCCTTGTTCATCTAAAAAGTAGTCGCCGGTa < 1:3152350/68‑1 (MQ=255)
aCATATTAAACACCGCGGTGCTTTTGGCTGTTGGCAATCCTTGTTCATCTAAAAAGTAGTCGCCGGTa < 1:1286120/68‑1 (MQ=255)
aCATATTAAACACCGCGGTGCTTTTGGCTGTTGGCAATCCTTGTTCATCTAAAAAGTAGTCGCCGGTa < 1:3064270/68‑1 (MQ=255)
aCATATTAAACACCGCGGTGCTTTTGGCTGTTGGCAATCCTTGTTCATCTAAAAAGTAGTCGCCGGTa < 1:1884327/68‑1 (MQ=255)
aCATATTAAACACCGCGGTGCTTTTGGCTGTTGGCAATCCTTGTTCATCTAAAAAGTAGTCGCCGGTa < 1:135252/68‑1 (MQ=255)
aCATATTAAACACCGCGGTGCTTTTGGCTGTTGGCAATCCTTGTTCATCTAAAAAGTAGTCGCCGGTa < 1:2495774/68‑1 (MQ=255)
aCATATTAAACACCGCGGTGCTTTTGGCTGTTGGCAATCCTTGTTCATCTAAAAAGTAGTCGCCGGTa < 1:1653344/68‑1 (MQ=255)
cATATTAAACACCGCGGTGCTTTTGGCTGTTGGCAATCCTTGTTCATCTAAAAAGTAGTCGCCGGTa < 1:1848398/67‑1 (MQ=255)
tatTAAACACCGCGGTGCTTTTGGCTGTTGGCAATCCTTGTTCATCTAAAAAGTAGTCGCCGGTa < 1:1219429/65‑1 (MQ=255)
atTAAACACCGCGGTGCTTTTGGCTGTTGGCAATCCTTGTTCATCTAAAAAGTAGTCGCCGGTAGAt > 1:943436/1‑67 (MQ=255)
atTAAACACCGCGGTGCTTTTGGCTGTTGGCAATCCTTGTTCATCTAAAAAGTAGTCGCCGGTAAAt > 1:3333754/1‑67 (MQ=255)
atTAAACACCGCGGTGCTTTTGGCTGTTGGCAATCCTTGTTCATCTAAAAAGTAGTCGCCGGTAAAt > 1:2885144/1‑67 (MQ=255)
atTAAACACCGCGGTGCTTTTGGCTGTTGGCAATCCTTGTTCATCTAAAAAGTAGTCGCCGGTAAAt > 1:1675085/1‑67 (MQ=255)
atTAAACACCGCGGTGCTTTTGGCTGTTGGCAATCCTTGTTCATCTAAAAAGTAGTCGCCGGTAAAt > 1:220917/1‑67 (MQ=255)
aaaCACCGCGGTGCTTTTGGCTGTTGGCAATCCTTGTTCATCTAAAAAGTAGTCGCCGGTAAATACCAg < 1:1908038/69‑1 (MQ=255)
aaaCACCGCGGTGCTTTTGGCTGTTGGCAATCCTTGTTCATCTAAAAAGTAGTCGCCGGTAAATACCAg < 1:1332116/69‑1 (MQ=255)
aaCACCGCGGTGCTTTTGGCTGTTGGCAATCCTTGTTCATCTa < 1:2000827/43‑1 (MQ=255)
cacCGCGGTGCTTTTGGCTGTTGGCAATCCTTGTTCATCTAAAAAGTAGTCGCCGGTa > 1:1795159/1‑58 (MQ=255)
cacCGCGGTGCTTTTGGCTGTTGGCAATCCTTGTTCATCTAAAAAGTAGTCGCCGGTa > 1:1933677/1‑58 (MQ=255)
cacCGCGGTGCTTTTGGCTGTTGGCAATCCTTGTTCATCTAAAAAGTAGTCGCCGGTa > 1:1300728/1‑58 (MQ=255)
cacCGCGGTGCTTTTGGCTGTTGGCAATCCTTGTTCATCTAAAAAGTAGTCGCCGGTa > 1:3112032/1‑58 (MQ=255)
cacCGCGGTGCTTTTGGCTGTTGGCAATCCTTGTTCATCTAAAAAGTAGTCGCCGGTa > 1:3184723/1‑58 (MQ=255)
ccGCGGTGCTTTTGGCTGTTGGCAATCCTTGTTCATCTAAAAAGTAGTCGCCGGTAAATACCAGCACGc < 1:3080970/69‑1 (MQ=255)
ccGCGGTGCTTTTGGCTGTTGGCAATCCTTGTTCATCTAAAAAGTAGGCGCCGGTAAATACCAGCACGc < 1:2649947/69‑1 (MQ=255)
|
AGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACATATTAAACACCGCGGTGCTTTTGGCTGTTGGCAATCCTTGTTCATCTAAAAAGTAGTCGCCGGTAAATACCAGCACGC > minE/869381‑869501
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
