Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F1 I0 R1
|
4577 |
57.4 |
3436498 |
77.7% |
2670158 |
63.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
876,263 |
G→A |
29.9% |
G493G (GGG→GGA) |
acnA → |
aconitate hydratase 1 |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 876,263 | 0 | G | A | 29.9%
| 79.6
/ 35.0
| 80 | G493G (GGG→GGA) | acnA | aconitate hydratase 1 |
| Reads supporting (aligned to +/- strand): ref base G (17/39); new base A (17/7); total (34/46) |
| Fisher's exact test for biased strand distribution p-value = 1.20e-03 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.92e-01 |
GGTTCGAAAGTCGTTTCTGATTATCTGGCAAAAGCGAAACTGACACCGTATCTCGACGAACTGGGGTTTAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGA > minE/876198‑876331
|
ggTTCGAAAGTCGTTTCTGATTATCTGGCAAAAGCGAAACTGACACCGTATCTCGACGAACTGGGGttt < 1:3303645/69‑1 (MQ=255)
ggTTCGAAAGTCGTTTCTGATTATCTGGCAAAAGCGAAACTGACACCGTATCTCGACGAACTGGGGttt < 1:2268351/69‑1 (MQ=255)
ggTTCGAAAGTCGTTTCTGATTATCTGGCAAAAGCGAAACTGACACCGTATCTCGACGAACTGGGGttt < 1:2552332/69‑1 (MQ=255)
ggTTCGAAAGTCGTTTCTGATTATCTGGCAAAAGCGAAACTGACACCGTATCTCGACGAACTGGGGttt < 1:721667/69‑1 (MQ=255)
tCGTTTCTGATTATCTGGCAAAAGCGAAACTGACACCGTATCTCGACGAACTGGGGTTTAACCTTGTgg < 1:412675/69‑1 (MQ=255)
tCGTTTCTGATTATCTGGCAAAAGCGAAACTGACACCGTATCTCGACGAACTGGGGTTTAACCTTGTgg < 1:3073527/69‑1 (MQ=255)
tCGTTTATGATTATCTGGCAAAAGCGAAACTGACACCGTATCTCGACGAACTGGGGTTTAACCTTGTgg < 1:1327335/69‑1 (MQ=255)
gTTTCTGATTATCTGGCAAAAGCGAAACTGACTCCGTATCTCGATGAACTGGGATTTAACCTTGTGGGa > 1:1132208/1‑69 (MQ=255)
gTTTCTGATTATCTGGCAAAAGCGAAACTGACACCGTATCTCGATGAACTGGGATTTAACCTTGTggg > 1:460025/1‑68 (MQ=255)
gTTTCTGATTATCTGGCAAAAGCGAAACTGACACCGTATCTCGATGAACTGGGATTTAACCTTGTGGGa > 1:1615814/1‑69 (MQ=255)
gTTTCTGATTATCTGGCAAAAGCGAAACTGACACCGTATCTCGATGAACTGGGATTTAACCTTGTGGGa > 1:1594954/1‑69 (MQ=255)
gTTTCTGATTATCTGGCAAAAGCGAAACTGACACCGTATCTCGATGAACTGGGATTTAACCTTGTGGGa > 1:2802219/1‑69 (MQ=255)
gTTTCTGATTATCTGGCAAAAGCGAAACTGACACCGTATCTCGATGAACTGGGATTTAACCTTGTGGGa > 1:446739/1‑69 (MQ=255)
gTTTCTGATTATCTGGCAAAAGCGAAACTGACACCGTATCTCGATGAACTGGGATTTAACCTTGTGGGa > 1:1724466/1‑69 (MQ=255)
gTTTCTGATTATCTGGCAAAAGCGAAACTGACACCGTATCTCGATGAACTGGGATTTAACCTTGTGGGa > 1:1778759/1‑69 (MQ=255)
gTTTCTGATTATCTGGCAAAAGCGAAACTGACACCGTATCTCGATGAACTGGGATTTAACCTTGTGGGa > 1:2311240/1‑69 (MQ=255)
gTTTCTGATTATCTGGCAAAAGCGAAACTGACACCGTATCTCGATGAACTGGGATTTAACCTTGTGGGa > 1:2050867/1‑69 (MQ=255)
gTTTCTGATTATCTGGCAAAAGCGAAACTGACACCGTATCTCGATGAACTGGGATTTAACCTTGTGGGa > 1:2154143/1‑69 (MQ=255)
gTTTCTGATTATCTGGCAAAAGCGAAACTGACACCGTATCTCGATGAACTGGGATTTAACCTTGTGGGa > 1:1010506/1‑69 (MQ=255)
gTTTCTGATTATCTGGCAAAAGCGAAACTGACACCGTATCTCGATGAACTGGGATTTAACCTTGTGGGa > 1:1159566/1‑69 (MQ=255)
gTTTCTGATTATCTGGCAAAAGCGAAACTGACACCGTATCTCGATGAACTGGGATTTAACCTTGTGGGa > 1:156093/1‑69 (MQ=255)
gTTTCTGATTATCTGGCAAAAGCGAAACTGACACCGTATCTCGATGAACTGGGATTTAACCTTGTGGGa > 1:3114323/1‑69 (MQ=255)
gTTTCTGATTATCTGGCAAAAGCGAAACTGACACCGTATCTCGATGAACTGGGATTTAACCTTGTGGGa > 1:944064/1‑69 (MQ=255)
gTTTCTGATTATCTGGCAAAAGCGAAACTGACACCGTATCTCGATGAACTGGGATTTAACCTTGTGGGa > 1:304942/1‑69 (MQ=255)
gTTTCTGATTATCTGGCAAAAGCGAAACTGACACCGTATCTCGACGAACTGGGGTTTAACCTTGTGGGa > 1:2882007/1‑69 (MQ=255)
gTTTCTGATTATCTGGCAAAAGCGAAACTGACACCGTATCTCGACGAACTGGGGTTTAACCTTGTGGGa > 1:824851/1‑69 (MQ=255)
tATCTGGCAAAAGCGAAACTTACACCGTATCTCGACGAACTGGGGTTTAACCTTGTGGGATACGGTTGt < 1:484118/69‑1 (MQ=255)
tATCTGGCAAAAGCGAAACTGACACCGTATCTCGACGAACTGGGGTTTAACCTTGTGGGATACGGt < 1:922244/66‑1 (MQ=255)
tATCTGGCAAAAGCGAAACTGACACCGTATCTCGACGAACTGGGGTTTAACCTTGTGGGATACGGt < 1:1377784/66‑1 (MQ=255)
tATCTGGCAAAAGCGAAACTGACACCGTATCTCGACGAACTGGGGTTTAACCTTGTGGGATACGGt < 1:1311753/66‑1 (MQ=255)
tATCTGGCAAAAGCGAAACTGACACCGTATCTCGACGAACTGGGGTTTAACCTTGTGGGATACGGt < 1:1974412/66‑1 (MQ=255)
tATCTGGCAAAAGCGAAACTGACACCGTATCTCGACGAACTGGGGTTTAACCTTGTGGGATACGGt < 1:467665/66‑1 (MQ=255)
tATCTGGCAAAAGCGAAACTGACACCGTATCTCGACGAACTGGGGTTTAACCTTGTGGGATACGGt < 1:2637498/66‑1 (MQ=255)
tATCTGGCAAAAGCGAAACTGACACCGTATCTCGACGAACTGGGGTTTAACCTTGTGGGATACGGTTGt < 1:3011809/69‑1 (MQ=255)
tATCTGGCAAAAGCGAAACTGACACCGTATCTCGACGAACTGGGGTTTAACCTTGTGGGATACGGTTGt < 1:3175654/69‑1 (MQ=255)
tATCTGGCAAAAGCGAAACTGACACCGTATCTCGACGAACTGGGGTTTAACCTTGTGGGATACGGTTGt < 1:1152599/69‑1 (MQ=255)
tATCTGGCAAAAGCGAAACTGACACCGTATCTCGACGAACTGGGGTTTAACCTTGTGGGATACGGTTGt < 1:121897/69‑1 (MQ=255)
tATCTGGCAAAAGCGAAACTGACACCGTATCTCGACGAACTGGGGTTTAACCTTGTGGGATACGGTTGt < 1:1903493/69‑1 (MQ=255)
tATCTGGCAAAAGCGAAACTGACACCGTATCTCGACGAACTGGGGTTTAACCTTGTGGGATACGGTTGt < 1:165820/69‑1 (MQ=255)
tATCTGGCAAAAGCGAAACTGACACCGTATCTCGACGAACTGGGG‑TTAACCTTGTGGGa > 1:1680618/1‑59 (MQ=255)
tATCTGGCAAAAGCGAAACTGACACCGTATCTCGACGAACTGGGG‑TTAACCTTGTGGGATACGGTTg > 1:1322761/1‑67 (MQ=255)
tATCTGGCAAAAGCGAAACTGACACCGTATCTCGACGAACTGGGG‑TTAACCTTGTGGGATACGGTTGt > 1:2047967/1‑68 (MQ=255)
tATCTGGCAAAAGCGAAACTGACACCGTATCTCGACGAACTGGGG‑TTAACCTTGTGGGATACGGTTGt > 1:1458512/1‑68 (MQ=255)
tATCTGGCAAAAGCGAAACTGACACCGTATCTCGACGAACTGGGG‑TTAACCTTGTGGGATACGGTTGt > 1:167346/1‑68 (MQ=255)
tATCTGGCAAAAGCGAAACTGACACCGTATCTCGACGAACTGGGG‑TTAACCTTGTGGGATACGGTTGt > 1:2442836/1‑68 (MQ=255)
tATCAGGCAAAAGCGAAACTGACACCGTATCTCGACGAACTGGGG‑TTAACCTTGTGGGATACGGTTGt > 1:1725104/1‑68 (MQ=255)
aTCTGGCAAAAGCGAAACTGACACCGTATCTCGACGAACTGGGGTTTAACCTTGTGGGATACGGTTGt < 1:1910633/68‑1 (MQ=255)
tCTGGCAAAAGCGAAACTGACACCGTATCTCGACGAACTGGGGTTTAACCTTGTGGGATACGGt < 1:2399914/64‑1 (MQ=255)
aaaaGCGAAACTGACACCGTATCTCGACGAACTGGGGTTTAACCTTGTGGGATACGGTTGTACCACCTg < 1:2774411/69‑1 (MQ=255)
aaaaGCGAAACTGACACCGTATCTCGACGAACTGGGGTTTAACCTTGTGGGATACGGTTGTACCACCTg < 1:2246190/69‑1 (MQ=255)
aaaaGCGAAACTGACACCGTATCTCGACGAACTGGGGTTTAACCTTGTGGGATACGGTTGTACCACCTg < 1:2359411/69‑1 (MQ=255)
aaaaGCGAAACTGACACCGTATCTCGACGAACTGGGGTTTAACCTTGTGGGATACGGTTGTACCACCTg < 1:1008022/69‑1 (MQ=255)
aaaaGCGAAACTGACACCGTATCTCGACGAACTGGGGTTTAACCTTGTGGGATACGGTTGTACCACCTg < 1:2593231/69‑1 (MQ=255)
aaaaGCGAAACTGACACCGTATCTCGACGAACTGGGGTTTAACCTTGTGGGATACGGTTGTACCACCTg < 1:2583051/69‑1 (MQ=255)
aaaaGCGAAACTGACACCGTATCTCGACGAACTGGGGTTTAACCTTGTGGGATACGGTTGTACCACCTg < 1:2494610/69‑1 (MQ=255)
aaaGCGAAACTGACACCGTATCTCGACGAACTGGGGGTTAACCTTGTGGGATACGGTTGTACCACCTGt < 1:3418912/69‑1 (MQ=255)
aaGCGAAACTGACACCGTATCTCGACGAACTGGGGTTTAACCTTGTGGGATACGGTTGTACCACCTGt < 1:1592279/68‑1 (MQ=255)
gCGAAACTGACACCGTATCTCGACGAACTGGGGttt < 1:2611971/36‑1 (MQ=255)
cTGACACCGTATCTCGACGAACTGGGGTTTAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAAc > 1:764086/1‑69 (MQ=255)
cTGACACCGTATCTCGACGAACTGGGGTTTAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAAc > 1:1428313/1‑69 (MQ=255)
tATCTCGACGAACTGGGGTTTAACCTTGTGGGATACGGTTGTACCACCTg < 1:1672745/50‑1 (MQ=255)
tcGACGAACTGGGGTTTAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGc > 1:2385924/1‑69 (MQ=255)
tcGACGAACTGGGGTTTAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGc > 1:3154823/1‑69 (MQ=255)
tcGACGAACTGGGGTTTAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGc > 1:3055483/1‑69 (MQ=255)
tcGACGAACTGGGGTTTAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGc > 1:176844/1‑69 (MQ=255)
tcGACGAACTGGGGTTTAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGc > 1:216869/1‑69 (MQ=255)
tcGACGAACTGGGGTTTAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGc > 1:2153826/1‑69 (MQ=255)
tgaACTGGGATTTAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGACCGTTGccc < 1:508288/66‑1 (MQ=255)
tgaACTGGGATTTAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGACCGTTGccc < 1:1390605/66‑1 (MQ=255)
tgaACTGGGATTTAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGACCGTTGccc < 1:1523588/66‑1 (MQ=255)
tgaACTGGGATTTAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGACCGTTGccc < 1:770327/66‑1 (MQ=255)
tgaACTGGGATTTAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGACCGTTGccc < 1:3160903/66‑1 (MQ=255)
tgaACTGGGATTTAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGACCGTTGccc < 1:2459569/66‑1 (MQ=255)
tgaACTGGGATTTAACCTTGTGGGATACGGTTGTACCACCTGTAATGGTAACTCTGGACCGTTGccc < 1:96470/66‑1 (MQ=255)
cTGGGGTTTAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCt < 1:584074/69‑1 (MQ=255)
cTGGGGTTTAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCt < 1:2339486/69‑1 (MQ=255)
cTGGGGTTTAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCt < 1:89091/69‑1 (MQ=255)
cTGGGGTTTAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCt < 1:2589979/69‑1 (MQ=255)
cTGGGGTTTAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCt < 1:1252034/69‑1 (MQ=255)
tGGGGTTTAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCt < 1:1620482/68‑1 (MQ=255)
gTTTAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGa < 1:492048/69‑1 (MQ=255)
gTTTAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGa < 1:454357/69‑1 (MQ=255)
|
GGTTCGAAAGTCGTTTCTGATTATCTGGCAAAAGCGAAACTGACACCGTATCTCGACGAACTGGGGTTTAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGA > minE/876198‑876331
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
