Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A2 F1 I0 R1 4577 57.4 3436498 77.7% 2670158 63.4

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 889,529 G→A 28.2% N155N (AAC→AAT)  fabI ← enoyl‑[acyl‑carrier‑protein] reductase, NADH‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE889,5290GA28.2% 138.9 / 54.2 127N155N (AAC→AAT) fabIenoyl‑[acyl‑carrier‑protein] reductase, NADH‑dependent
Reads supporting (aligned to +/- strand):  ref base G (63/28);  new base A (24/12);  total (87/40)
Fisher's exact test for biased strand distribution p-value = 8.33e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.06e-01

Only 100 of 134 total aligned reads displayed.

GCGTTCGCCATATAGCGCACGTTCGCTTCCAGAGACGCTTTTGCCAGACCCATAACGTTGTAGTTCGGGATAGCGCGCTCAGCGCCAAGGTAGGAAAGGGTCAGCAGGGCAGAACCCGGATTCAGCATGGA  >  minE/889467‑889597
                                                              |                                                                    
gcgTTCGCCATATAGCGCACGTTCGCTTCCAGAGACGCTTTTGCCAGACCCATAACGTTGTAGTTCgg                                                                 >  1:2113487/1‑68 (MQ=255)
gcgTTCGCCATATAGCGCACGTTCGCTTCCAGAGACGCTTTTGCCAGACCCATAACGTTGTAGTTCgg                                                                 >  1:2139649/1‑68 (MQ=255)
gcgTTCGCCATATAGCGCACGTTCGCTTCCAGAGACGCTTTTGCCAGACCCATAACGTTGTAGTTCgg                                                                 >  1:3327944/1‑68 (MQ=255)
gcgTTCGCCATATAGCGCACGTTCGCTTCCAGAGACGCTTTTGCCAGACCCATAACGTTGTAGTTCgg                                                                 >  1:2510018/1‑68 (MQ=255)
gcgTTCGCCATATAGCGCACGTTCGCTTCCAGAGACGCTTTTGCCAGACCCATAACGTTGTAGTTCgg                                                                 >  1:367650/1‑68 (MQ=255)
gcgTTCGCCATATAGCGCACGTTCGCTTCCAGAGACGCTTTTGCCAGACCCATAACGTTGTAGTTCgg                                                                 >  1:23794/1‑68 (MQ=255)
gcgTTCGCCATATAGCGCACGTTCGCTTCCAGAGACGCTTTTGCCAGACCCATAACGTTGTAGTTCg                                                                  >  1:2205618/1‑67 (MQ=255)
gcgTTCGCCATATAGCGCACGTTCGCTTCCAGAGACGCTTTTGCCAGACCCATAACGTT‑TAGTTCgg                                                                 >  1:2748327/1‑67 (MQ=255)
            taGCGCACGTTCGCTTCGAGAGACGCTTTTGCCAGACCCATAACGTTGTAGTTCGGGATAGCGCGCTCa                                                    >  1:712115/1‑69 (MQ=255)
            taGCGCACGTTCGCTTCCAGAGACGCTTTTGCCAGACCCATAACGTTGTAGTTCGGGa                                                               >  1:2621516/1‑58 (MQ=255)
            taGCGCACGTTCGCTTCCAGAGACGCTTTTGCCAGACCCATAACGTTGTAGTTCGGGa                                                               >  1:2135470/1‑58 (MQ=255)
            taGCGCACGTTCGCTTCCAGAGACGCTTTTGCCAGACCCATAACGTTGTAGTTCGGGa                                                               >  1:1875198/1‑58 (MQ=255)
            taGCGCACGTTCGCTTCCAGAGACGCTTTTGCCAGACCCATAACGTTGTAGTTCGGGa                                                               >  1:341977/1‑58 (MQ=255)
            taGCGCACGTTCGCTTCCAGAGACGCTTTTGCCAGACCCATAACGTTGTAGTTCGGGa                                                               >  1:2879609/1‑58 (MQ=255)
            taGCGCACGTTCGCTTCCAGAGACGCTTTTGCCAGACCCATAACGTTGTAGTTCGGGa                                                               >  1:2367535/1‑58 (MQ=255)
            taGCGCACGTTCGCTTCCAGAGACGCTTTTGCCAGACCCATAACGTTGTAGTTCGGGa                                                               >  1:1588754/1‑58 (MQ=255)
            taGCGCACGTTCGCTTCCAGAGACGCTTTTGCCAGACCCATAACGTTGTAGTTCGGGa                                                               >  1:1462897/1‑58 (MQ=255)
            taGCGCACGTTCGCTTCCAGAGACGCTTTTGCCAGACCCATAACGTTGTAGTTCGGGa                                                               >  1:2835451/1‑58 (MQ=255)
            taGCGCACGTTCGCTTCCAGAGACGCTTTTGCCAGACCCATAACGTTGTAGTTCGGGa                                                               >  1:1176390/1‑58 (MQ=255)
            taGCGCACGTTCGCTTCCAGAGACGCTTTTGCCAGACCCATAACGTTGTAGTTCGGGa                                                               >  1:2794966/1‑58 (MQ=255)
            taGCGCACGTTCGCTTCCAGAGACGCTTTTGCCAGACCCATAACGTTGTAGTTCGGGa                                                               >  1:1116476/1‑58 (MQ=255)
            taGCGCACGTTCGCTTCCAGAGACGCTTTTGCCAGACCCATAACGTTGTAGTTCGGGATAGCGCGCTc                                                     >  1:1549620/1‑68 (MQ=255)
            taGCGCACGTTCGCTTCCAGAGACGCTTTTGCCAGACCCATAACGTTGTAGTTCGGGATAGCGCGCTc                                                     >  1:656596/1‑68 (MQ=255)
            taGCGCACGTTCGCTTCCAGAGACGCTTTTGCCAGACCCATAACGTTGTAGTTCGGGATAGCGCGCTc                                                     >  1:3305376/1‑68 (MQ=255)
            taGCGCACGTTCGCTTCCAGAGACGCTTTTGCCAGACCCATAACGTTGTAGTTCGGGATAGCGCGCTCa                                                    >  1:2869783/1‑69 (MQ=255)
            taGCGCACGTTCGCTTCCAGAGACGCTTTTGCCAGACCCATAACGTTGTAGTTCGGGATAGCGCGCTCa                                                    >  1:2460114/1‑69 (MQ=255)
            taGCGCACGTTCGCTTCCAGAGACGCTTTTGCCAGACCCATAACGTTGTAGTTCGGGATAGCGCGCTCa                                                    >  1:1351769/1‑69 (MQ=255)
            taGCGCACGTTCGCTTCCAGAGACGCTTTTGCCAGACCCATAACGTTGTAGTTCGGGATAGCGCGCTCa                                                    >  1:1808591/1‑69 (MQ=255)
                    gTTCGCTTCCAGAGACGCTTTTGCCAGACCCATAACGTTGTAGTTCGGGATAGCGCGCTCAGCGCCaa                                             >  1:2575197/1‑68 (MQ=255)
                                 gaCGCTTTTGCCAGACCCATAACGTTGTAGTTCGGGATAGCGCGCTCAGCGCCAAGGTAGGAAAGGGTc                               >  1:2545406/1‑69 (MQ=255)
                                 gaCGCTTTTGCCAGACCCATAACGTTGTAGTTCGGGATAGCGCGCTCAGCGCCAAGGTAGGAAAGGGTc                               >  1:938005/1‑69 (MQ=255)
                                 gaCGCTTTTGCCAGACCCATAACGTTGTAGTTCGGGATAGCGCGCTCAGCGCCAAGGTAGGAAAGGGTc                               >  1:67369/1‑69 (MQ=255)
                                                    tAACGTTGTAATTCGGGATAGCGCGCTCAGCGCCAAGGTAGGAAAGGGTCAGCAGGGCAGAACCCGGa             <  1:1039381/68‑1 (MQ=255)
                                                    tAACGTTGTAATTCGGGATAGCGCGCTCAGCGCCAAGGTAGGAAAGGGTCAGCAGGGCAGAACCCGGa             <  1:1152524/68‑1 (MQ=255)
                                                    tAACGTTGTAATTCGGGATAGCGCGCTCAGCGCCAAGGTAGGAAAGGGTCAGCAGGGCAGAACCCGGa             <  1:878739/68‑1 (MQ=255)
                                                    tAACGTTGTAATTCGGGATAGCGCGCTCAGCGCCAAGGTAGGAAAGGGTCAGCAGGGCAGAACCCGGa             <  1:1354352/68‑1 (MQ=255)
                                                    tAACGTTGTAATTCGGGATAGCGCGCTCAGCGCCAAGGTAGGAAAGGGTCAGCAGGGCAGAACCCGGa             <  1:1424618/68‑1 (MQ=255)
                                                    tAACGTTGTAATTCGGGATAGCGCGCTCAGCGCCAAGGTAGGAAAGGGTCAGCAGGGCAGAACCCGGa             <  1:1926055/68‑1 (MQ=255)
                                                    tAACGTTGTAATTCGGGATAGCGCGCTCAGCGCCAAGGTAGGAAAGGGTCAGCAGGGCAGAACCCGGa             <  1:1810958/68‑1 (MQ=255)
                                                    tAACGTTGTAATTCGGGATAGCGCGCTCAGCGCCAAGGTAGGAAAGGGTCAGCAGGGCAGAACCCGGa             <  1:2906771/68‑1 (MQ=255)
                                                    tAACGTTGTAATTCGGGATAGCGCGCTCAGCGCCAAGGTAGGAAAGGGTCAGCAGGGCAGAACCCGGa             <  1:3358972/68‑1 (MQ=255)
                                                    tAACGTTGTAATTCGGGATAGCGCGCTCAGCGCCAAGGTAGGAAAGGGTCAGCAGGGCAGAACCCGGa             <  1:3421759/68‑1 (MQ=255)
                                                    tAACGTTGTAATTCGGGATAGCGCGCTCAGCGCCAAGGTAGGAAAGGGTCAGCAGGGCAGAACCCGGa             <  1:2866685/68‑1 (MQ=255)
                                                      tcGTTGTAATTCGGGATAGCGCGCTCAGCGCCAAGGTAGGAAAGGGTCAGCAGGGCAGAACCCGGATTc          >  1:274504/2‑69 (MQ=255)
                                                      aCGTTGTAGTTCGGGATAGCGCGCTCAGCGCCAAGGTAGGAAAgg                                  <  1:1631919/45‑1 (MQ=255)
                                                      aCGTTGTAGTTCGGGATAGCGCGCTCAGCGCCAAGGTAGGAAAgg                                  <  1:618101/45‑1 (MQ=255)
                                                      aCGTTGTAGTTCGGGATAGCGCGCTCAGCGCCAAGGTAGGAAAgg                                  <  1:3242802/45‑1 (MQ=255)
                                                      aCGTTGTAGTTCGGGATAGCGCGCTCAGCGCCAAGGTAGGAAAgg                                  <  1:2003532/45‑1 (MQ=255)
                                                      aCGTTGTAGTTCGGGATAGCGCGCTCAGCGCCAAGGTAGGAAAgg                                  <  1:3120017/45‑1 (MQ=255)
                                                      aCGTTGTAGTTCGGGATAGCGCGCTCAGCGCCAAGGTAGGAAAGGGTCAGCAGGGCAGAACCCGGATTc          >  1:3209968/1‑69 (MQ=255)
                                                      aCGTTGTAGTTCGGGATAGCGCGCTCAGCGCCAAGGTAGGAAAGGGTCAGCAGGGCAGAACCCGGATTc          <  1:3118729/69‑1 (MQ=255)
                                                      aCGTTGTAGTTCGGGATAGCGCGCTCAGCGCCAAGGTAGGAAAGGGTCAGCAGGGCAGAACCCGGATTc          >  1:722972/1‑69 (MQ=255)
                                                      aCGTTGTAGTTCGGGATAGCGCGCTCAGCGCCAAGGTAGGAAAGGGTCAGCAGGGCAGAACCCGGATTc          <  1:752839/69‑1 (MQ=255)
                                                      aCGTTGTAGTTCGGGATAGCGCGCTCAGCGCCAAGGTAGGAAAGGGTCAGCAGGGCAGAACCCGGATTc          <  1:3175201/69‑1 (MQ=255)
                                                      aCGTTGTAGTTCGGGATAGCGCGCTCAGCGCCAAGGTAGGAAAGGGTCAGCAGGGCAGAACCCGGATTc          >  1:1027080/1‑69 (MQ=255)
                                                      aCGTTGTAGTTCGGGATAGCGCGCTCAGCGCCAAGGTAGGAAAGGGTCAGCAGGGCAGAACCCGGATTc          <  1:3177376/69‑1 (MQ=255)
                                                      aCGTTGTAGTTCGGGATAGCGCGCTCAGCGCCAAGGTAGGAAAGGGTCAGCAGGGCAGAACCCGGATTc          >  1:2616391/1‑69 (MQ=255)
                                                      aCGTTGTAGTTCGGGATAGCGCGCTCAGCGCCAAGGTAGGAAAGGGTCAGCAGGGCAGAACCCGGATTc          <  1:1303887/69‑1 (MQ=255)
                                                      aCGTTGTAGTTCGGGATAGCGCGCTCAGCGCCAAGGTAGGAAAGGGTCAGCAGGGCAGAACCCGGATTc          >  1:1741534/1‑69 (MQ=255)
                                                      aCGTTGTAGTTCGGGATAGCGCGCTCAGCGCCAAGGTAGGAAAGGGTCAGCAGGGCAGAACCCGGATTc          >  1:171155/1‑69 (MQ=255)
                                                      aCGTTGTAGTTCGGGATAGCGCGCTCAGCGCCAAGGTAGGAAAGGGTCAGCAGGGCAGAACCCGGATTc          >  1:1675357/1‑69 (MQ=255)
                                                      aCGTTGTAGTTCGGGATAGCGCGCTCAGCGCCAAGGTAGGAAAGGGTCAGCAGGGCAGAACCCGGATTc          <  1:2069905/69‑1 (MQ=255)
                                                      aCGTTGTAATTCGGGATAGCGCGCTCAGCGCCAAGGTAGGAAAGGGTCAGTAGGGCAGAACCCGGATTc          >  1:2231674/1‑69 (MQ=255)
                                                      aCGTTGTAATTCGGGATAGCGCGCTCAGCGCCAAGGTAGGAAAGGGTCAGCAGGGCAGAACCCGGATTc          >  1:1621976/1‑69 (MQ=255)
                                                      aCGTTGTAATTCGGGATAGCGCGCTCAGCGCCAAGGTAGGAAAGGGTCAGCAGGGCAGAACCCGGATTc          >  1:3317858/1‑69 (MQ=255)
                                                      aCGTTGTAATTCGGGATAGCGCGCTCAGCGCCAAGGTAGGAAAGGGTCAGCAGGGCAGAACCCGGATTc          >  1:3355234/1‑69 (MQ=255)
                                                      aCGTTGTAATTCGGGATAGCGCGCTCAGCGCCAAGGTAGGAAAGGGTCAGCAGGGCAGAACCCGGATTc          >  1:3369561/1‑69 (MQ=255)
                                                      aCGTTGTAATTCGGGATAGCGCGCTCAGCGCCAAGGTAGGAAAGGGTCAGCAGGGCAGAACCCGGATTc          >  1:1685316/1‑69 (MQ=255)
                                                      aCGTTGTAATTCGGGATAGCGCGCTCAGCGCCAAGGTAGGAAAGGGTCAGCAGGGCAGAACCCGGATTc          >  1:1014257/1‑69 (MQ=255)
                                                      aCGTTGTAATTCGGGATAGCGCGCTCAGCGCCAAGGTAGGAAAGGGTCAGCAGGGCAGAACCCGGATTc          >  1:624649/1‑69 (MQ=255)
                                                      aCGTTGTAATTCGGGATAGCGCGCTCAGCGCCAAGGTAGGAAAGGGTCAGCAGGGCAGAACCCGGATTc          >  1:1502499/1‑69 (MQ=255)
                                                      aCGTTGTAATTCGGGATAGCGCGCTCAGCGCCAAGGTAGGAAAGGGTCAGCAGGGCAGAACCCGGATTc          >  1:827734/1‑69 (MQ=255)
                                                      aCGTTGTAATTCGGGATAGCGCGCTCAGCGCCAAGGTAGGAAAGGGTCAGCAGGGCAGAACCCGGATTc          >  1:1243757/1‑69 (MQ=255)
                                                      aCGTTGTAATTCGGGATAGCGCGCTCAGCGCCAAGGTAGGAAAGGGTCAGCAGGGCAGAACCCGGATTc          >  1:3237427/1‑69 (MQ=255)
                                                      aCGTTGTAATTCGGGATAGCGCGCTCAGCGCCAAGGTAGGAAAGGGTCAGCAGGGCAGAACCCGGATTc          >  1:1965849/1‑69 (MQ=255)
                                                      aCGTTGTAATTCGGGATAGCGCGCTCAGCGCCAAGGTAGGAAAGGGTCAGCAGGGCAGAACCCGGATTc          >  1:2568033/1‑69 (MQ=255)
                                                      aCGTTGTAATTCGGGATAGCGCGCTCAGCGCCAAGGTAGGAAAGGGTCAGCAGGGCAGAACCCGGATTc          >  1:1966922/1‑69 (MQ=255)
                                                      aCGTTGTAATTCGGGATAGCGCGCTCAGCGCCAAGGTAGGAAAGGGTCAGCAGGGCAGAACCCGGATTc          >  1:1998768/1‑69 (MQ=255)
                                                      aCGTTGTAATTCGGGATAGCGCGCTCAGCGCCAAGGTAGGAAAGGGTCAGCAGGGCAGAACCCGGATTc          >  1:2000061/1‑69 (MQ=255)
                                                      aCGTTGTAATTCGGGATAGCGCGCTCAGCGCCAAGGTAGGAAAGGGTCAGCAGGGCAGAACCCGGATTc          >  1:3105820/1‑69 (MQ=255)
                                                      aCGTTGTAATTCGGGATAGCGCGCTCAGCGCCAAGGTAGGAAAGGGTCAGCAGGGCAGAACCCGGATTc          >  1:2306912/1‑69 (MQ=255)
                                                      aCGTTGTAATTCGGGATAGCGCGCTCAGCGCCAAGGTAGGAAAGGGTCAGCAGGGCAGAACCCGGATTc          >  1:2584071/1‑69 (MQ=255)
                                                      aCGTTGTAATTCGGGATAGCGCGCTCAGCGCCAAGGTAGGAAAGGGTCAGCAGGGCAGAACACGGATTc          >  1:478611/1‑69 (MQ=255)
                                                        gTTGTAGTTCGGGATAGCGCGCTCAGCGCCAAGGTAGGAAAGGGTCAGCAGGGCAGAACCCGGATTCAg        <  1:468754/69‑1 (MQ=255)
                                                        gTTGTAGTTCGGGATAGCGCGCTCAGCGCCAAGGTAGGAAAGGGTCAGCAGGGCAGAACCCGGATTCAg        <  1:1346109/69‑1 (MQ=255)
                                                        gTTGTAGTTCGGGATAGCGCGCTCAGCGCCAAGGTAGGAAAGGGTCAGCAGGGCAGAACCCGGATTCAg        <  1:6912/69‑1 (MQ=255)
                                                        gTTGTAGTTCGGGATAGCGCGCTCAGCGCCAAGGTAGGAAAGGGTCAGCAGGGCAGAACCCGGATTCAg        <  1:872328/69‑1 (MQ=255)
                                                        gTTGTAGTTCGGGATAGCGCGCTCAGCGCCAAGGTAGGAAAGGGTCAGCAGGGCAGAACCCGGATTCAg        <  1:490853/69‑1 (MQ=255)
                                                        gTTGTAGTTCGGGATAGCGCGCTCAGCGCCAAGGTAGGAAAGGGTCAGCAGGGCAGAACCCGGATTCAg        <  1:3193455/69‑1 (MQ=255)
                                                        gTTGTAGTTCGGGATAGCGCGCTCAGCGCCAAGGTAGGAAAGGGTCAGCAGGGCAGAACCCGGATTCAg        <  1:179115/69‑1 (MQ=255)
                                                            tAGTTCGGGATAGCGCGCTCAGCGCCAAGGTAGGAAAGGGTCAGCAGGGCAGAACCCGGATTCAGCa      >  1:1280402/1‑67 (MQ=255)
                                                            tAGTTCGGGATAGCGCGCTCAGCGCCAAGGTAGGAAAGGGTCAGCAGGGCAGAACCCGGATTCAGCa      >  1:1874328/1‑67 (MQ=255)
                                                            tAGTTCGGGATAGCGCGCTCAGCGCCAAGGTAGGAAAGGGTCAGCAGGGCAGAACCCGGATTCAGCa      >  1:2646116/1‑67 (MQ=255)
                                                            tAGTTCGGGATAGCGCGCTCAGCGCCAAGGTAGGAAAGGGTCAGCAGGGCAGAACCCGGATTCAGCa      >  1:1444190/1‑67 (MQ=255)
                                                            tAGTTCGGGATAGCGCGCTCAGCGCCAAGGTAGGAAAGGGTCAGCAGGGCAGAACCCGGATTCAGCa      >  1:2149138/1‑67 (MQ=255)
                                                            tAGTTCGGGATAGCGCGCTCAGCGCCAAGGTAGGAAAGGGTCAGCAGGGCAGAACCCGGATTCAGCa      >  1:196048/1‑67 (MQ=255)
                                                            tAGTTCGGGATAGCGCGCTCAGCGCCAAGGTAGGAAAGGATCAGCAGGGCAGAACCCGGATTCAGCa      >  1:2712522/1‑67 (MQ=255)
                                                              gTTCGGGATAGCGCGCTCAGCGCCAAGGTAGGAAAGGGTCAGCAggg                        >  1:91869/1‑47 (MQ=255)
                                                              gTTCGGGATAGCGCGCTCAGCGCCAAGGTAGGAAAGGGTCAGCAGGGCAGAACCCGGATTCAGCATgg   <  1:2272413/68‑1 (MQ=255)
                                                              gTTCGGGATAGCGCGCTCAGCGCCAAGGTAGGAAAGGGTCAGCAGGGCAGAACCCGGATTCAGCATGGa  >  1:64606/1‑69 (MQ=255)
                                                              |                                                                    
GCGTTCGCCATATAGCGCACGTTCGCTTCCAGAGACGCTTTTGCCAGACCCATAACGTTGTAGTTCGGGATAGCGCGCTCAGCGCCAAGGTAGGAAAGGGTCAGCAGGGCAGAACCCGGATTCAGCATGGA  >  minE/889467‑889597

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A