Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F1 I0 R1
|
4577 |
57.4 |
3436498 |
77.7% |
2670158 |
63.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
889,796 |
T→G |
47.9% |
A66A (GCA→GCC) |
fabI ← |
enoyl‑[acyl‑carrier‑protein] reductase, NADH‑dependent |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 889,796 | 0 | T | G | 47.9%
| ‑3.3
/ 85.3
| 69 | A66A (GCA→GCC) | fabI | enoyl‑[acyl‑carrier‑protein] reductase, NADH‑dependent |
| Reads supporting (aligned to +/- strand): ref base T (19/17); new base G (25/8); total (44/25) |
| Fisher's exact test for biased strand distribution p-value = 7.84e-02 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
AACCGTCAAATTTCGGCCAAACTTTCCCCAGTTCAGCGAACATGGTGTCGATGCTGGCATCTTCTGCAACATCGCACTGCAGAACGATGTCAGAACCCAATTGAGCGGCAAATTCTTCTACGCGGCCT > minE/889732‑889859
|
aaCCGTCAAATTTCGGCCAAACTTTCCCCAGTTCAGCGAACATGGTGTCGATGCTGGCATCTTCTGCaa < 1:869366/69‑1 (MQ=255)
aCCGTCAAATTTCGGCCAAACTTTCCCCAGTTCAGCGAACATGGTGTCGATGCTGGCATCTTCTGCAAc < 1:2917772/69‑1 (MQ=255)
aCCGTCAAATTTCGGCCAAACTTTCCCCAGTTCAGCGAACATGGTGTCGATGCTGGCATCTTCTGCAAc < 1:464317/69‑1 (MQ=255)
aaaTTTCGGCCAAACTTTCCCCAGTTCAGCGAACATGGTGTCGATGCTGGCATCTTCTGCAACATCGCa < 1:1006793/69‑1 (MQ=255)
aaaTTTCGGCCAAACTTTCCCCAGTTCAGCGAACATGGTGTCGATGCTGGCATCTTCTGCAACATCGCa < 1:2110659/69‑1 (MQ=255)
aaaTTTCGGCCAAACTTTCCCCAGTTCAGCGAACATGGTGTCGATGCTGGCATCTTCTGCAACATCGCa < 1:352618/69‑1 (MQ=255)
aaaTTTCGGCCAAACTTTCCCCAGTTCAGCGAACATGGTGTCGATGCTGGCATCTTCTGCAACATCGCa < 1:2016248/69‑1 (MQ=255)
aaaTTTCGGCCAAACTTTCCCCAGTTCAGCGAACATGGTGTCGATGCTGGCATCTTCTGCAACATCGCa < 1:1896667/69‑1 (MQ=255)
aaaTTTCGGCCAAACTTTCCCCAGTTCAGCGAACATGGTGTCGATGCTGGCATCTTCTGCAACATCGCa < 1:116362/69‑1 (MQ=255)
aaaCTTTCCCCAGTTCATCGAACATGGTGTCGATGCTGGTATCTTCGGCAACATCGCACTGCAGAACGa > 1:3130281/1‑69 (MQ=255)
aaaCTTTCCCCAGTTCAGCGATCATGGTGTCGATGCTGGTATCTTCGGCAACATCGCACTGCAGAACGa > 1:1522266/1‑69 (MQ=255)
aaaCTTTCCCCAGTTCAGCGAACATGGTGTCGATGCTGGTATCTTCGGCAACAt > 1:1242833/1‑54 (MQ=255)
aaaCTTTCCCCAGTTCAGCGAACATGGTGTCGATGCTGGTATCTTCGGCAACATCGCACt > 1:276150/1‑60 (MQ=255)
aaaCTTTCCCCAGTTCAGCGAACATGGTGTCGATGCTGGTATCTTCGGCAACATCGCACTTCAGAACGa > 1:2563975/1‑69 (MQ=255)
aaaCTTTCCCCAGTTCAGCGAACATGGTGTCGATGCTGGTATCTTCGGCAACATCGCACTGCAGAACGa > 1:561661/1‑69 (MQ=255)
aaaCTTTCCCCAGTTCAGCGAACATGGTGTCGATGCTGGTATCTTCGGCAACATCGCACTGCAGAACGa > 1:3050890/1‑69 (MQ=255)
aaaCTTTCCCCAGTTCAGCGAACATGGTGTCGATGCTGGTATCTTCGGCAACATCGCACTGCAGAACGa > 1:2850506/1‑69 (MQ=255)
aaaCTTTCCCCAGTTCAGCGAACATGGTGTCGATGCTGGTATCTTCGGCAACATCGCACTGCAGAACGa > 1:2953023/1‑69 (MQ=255)
aaaCTTTCCCCAGTTCAGCGAACATGGTGTCGATGCTGGTATCTTCGGCAACATCGCACTGCAGAACGa < 1:2363410/69‑1 (MQ=255)
aaaCTTTCCCCAGTTCAGCGAACATGGTGTCGATGCTGGTATCTTCGGCAACATCGCACTGCAGAACGa > 1:2410700/1‑69 (MQ=255)
aaaCTTTCCCCAGTTCAGCGAACATGGTGTCGATGCTGGTATCTTCGGCAACATCGCACTGCAGAACGa > 1:2831509/1‑69 (MQ=255)
aaaCTTTCCCCAGTTCAGCGAACATGGTGTCGATGCTGGTATCTTCGGCAACATCGCACTGCAGAACGa < 1:242215/69‑1 (MQ=255)
aaaCTTTCCCCAGTTCAGCGAACATGGTGTCGATGCTGGTATCTTCGGCAACATCGCACTGCAGAACGa > 1:2807076/1‑69 (MQ=255)
aaaCTTTCCCCAGTTCAGCGAACATGGTGTCGATGCTGGTATCTTCGGCAACATCGCACTGCAGAACGa > 1:3256744/1‑69 (MQ=255)
aaaCTTTCCCCAGTTCAGCGAACATGGTGTCGATGCTGGTATCTTCGGCAACATCGCACTGCAGAACGa > 1:2636983/1‑69 (MQ=255)
aaaCTTTCCCCAGTTCAGCGAACATGGTGTCGATGCTGGTATCTTCGGCAACATCGCACTGCAGAACGa < 1:2671601/69‑1 (MQ=255)
aaaCTTTCCCCAGTTCAGCGAACATGGTGTCGATGCTGGTATCTTCGGCAACATCGCACTGCAGAACGa > 1:595883/1‑69 (MQ=255)
aaaCTTTCCCCAGTTCAGCGAACATGGTGTCGATGCTGGTATCTTCGGCAACATCGCACTGCAGAACGa < 1:1236764/69‑1 (MQ=255)
aaaCTTTCCCCAGTTCAGCGAACATGGTGTCGATGCTGGTATCTTCGGCAACATCGCACTGCAGAACGa < 1:3330384/69‑1 (MQ=255)
aaaCTTTCCCCAGTTCAGCGAACATGGTGTCGATGCTGGTATCTTCGGCAACATCGCACTGCAGAACGa < 1:3353945/69‑1 (MQ=255)
aaaCTTTCCCCAGTTCAGCGAACATGGTGTCGATGCTGGTATCTTCGGCAACATCGCACTGCAGAACGa > 1:164207/1‑69 (MQ=255)
aaaCTTTCCCCAGTTCAGCGAACATGGTGTCGATGCTGGTATCTTCGGCAACATCGCACTGCAGAACGa > 1:867180/1‑69 (MQ=255)
aaaCTTTCCCCAGTTCAGCGAACATGGTGTCGATGCTGGTATCTTCGGCAACATCGCACTGCAGAACGa < 1:1687085/69‑1 (MQ=255)
aaaCTTTCCCCAGTTCAGCGAACATGGTGTCGATGCTGGTATCTTCGGCAACATCGCACTGCAGAACGa > 1:1708393/1‑69 (MQ=255)
aaaCTTTCCCCAGTTCAGCGAACATGGTGTCGATGCTGGTATCTTCGGCAACATCGCACTGCAGAACGa < 1:1785693/69‑1 (MQ=255)
aaaCTTTCCCCAGTTCAGCGAACATGGTGTCGATGCTGGTATCTTCGGCAACATCGCACTGCAGAACGa > 1:1871937/1‑69 (MQ=255)
aaaCTTTCCCCAGTTCAGCGAACATGGTGTCGATGCTGGTATCTTCGGCAACATCGCACTGCAGAACGa > 1:831865/1‑69 (MQ=255)
aaaCTTTCCCCAGTTCAGCGAACATGGTGTCGATGCTGGTATCTTCGGCAACATCGCACTGCAGAACGa > 1:1995353/1‑69 (MQ=255)
aaaCTTTCCCCAGTTCAGCGAACATGGTGTCGATGCTGGTATCTTCGGCAACATCGCACTGCAGAACGa > 1:2110739/1‑69 (MQ=255)
aaaCTTTCCCCAGTTCAGCGAACATGGTGTCGATGCAGGTATCTTCGGCAACATCGCACTGCAGAACGa > 1:1614268/1‑69 (MQ=255)
aaaCTTT‑CCCAGTTCAGCGAACATGGTGTCGATGCTGGTATCTTCGGCAACATCGCACTGCAGAACGAt > 1:1995042/1‑69 (MQ=255)
aaaCTTACCCCAGTTCAGCGAACATGGTGTCGATGCTGGTATCTTCGGCAACATCGCACTGCAGAACGa > 1:4953/1‑69 (MQ=255)
cTTTCCCCAGTTCAGCGAACATGGTGTCGATGCTGGCATCTTCTGCAACATCGCACTGCAGAACGAt > 1:3108377/1‑67 (MQ=255)
cTTTCCCCAGTTCAGCGAACATGGTGTCGATGCTGGCATCTTCTGCAACATCGCACTGCAGAACGAt > 1:2936172/1‑67 (MQ=255)
cTTTCCCCAGTTCAGCGAACATGGTGTCGATGCTGGCATCTTCTGCAACATCGCACTGCAGAACGAt > 1:2325537/1‑67 (MQ=255)
cTTTCCCCAGTTCAGCGAACATGGTGTCGATGCTGGCATCTTCTGCAACATCGCACTGCAGAACGAt > 1:2874749/1‑67 (MQ=255)
cTTTCCCCAGTTCAGCGAACATGGTGTCGATGCTGGCATCTTCTGCAACATCGCACTGCAGAACGAt > 1:2501789/1‑67 (MQ=255)
cTTTCCCCAGTTCAGCGAACATGGTGTCGATGCTGGCATCTTCTGCAACATCGCACTGCAGAACGAt > 1:2261933/1‑67 (MQ=255)
cTTTCCCCAGTTCAGCGAACATGGTGTCGATGCTGGCATCTTCTGCAACATCGCACTGCAGAACGAt > 1:2251660/1‑67 (MQ=255)
cTTTCCCCAGTTCAGCGAACATGGTGTCGATGCTGGCATCTTCTGCAACATCGCACTGCAGAACGAt > 1:2073323/1‑67 (MQ=255)
cTTTCCCCAGTTCAGCGAACATGGTGTCGATGCTGGCATCTTCTGCAACATCGCACTGCAGAACGAt > 1:1597131/1‑67 (MQ=255)
cTTTCCCCAGTTCAGCGAACATGGTGTCGATGCTGGCATCTTCTGCAACATCGCACTGCAGAACGAt > 1:12453/1‑67 (MQ=255)
tCAGCGAACATGGTGTCGATGCTGGCATCTTCTGCAACAt > 1:3023272/1‑40 (MQ=255)
tCAGCGAACATGGTGTCGATGCTGGCATCTTCTGCAACATCGCACTGCATAACGATGTCAGAACCCaa > 1:997852/1‑68 (MQ=255)
tCAGCGAACATGGTGTCGATGCTGGCATCTTCTGCAACATCGCACTGCAGAACGATGTCAGAACCCaa > 1:1466271/1‑68 (MQ=255)
cAGCGAACATGGTTTCGATGCTGTGATCTTCTGCAACATGGCa < 1:2899841/43‑1 (MQ=25)
gATGCTGGCATCTTCTGCAACATCGCACTGCAGAACGATGTCAGAACCCAATTGAGCGGCAAAttcttc > 1:2601049/1‑69 (MQ=255)
gATGCTGGCATCTTCTGCAACATCGCACTGCAGAACGATGTCAGAACCCAATTGAGCGGCAAAttcttc > 1:3435165/1‑69 (MQ=255)
gATGCTGGCATCTTCTGCAACATCGCACTGCAGAACGATGTCAGAACCCAATTGAGCGGCAAAttcttc > 1:3226571/1‑69 (MQ=255)
gATGCTGGCATCTTCTGCAACATCGCACTGCAGAACGATGTCAGAACCCAATTGAGCGGCAAAttcttc > 1:296156/1‑69 (MQ=255)
gATGCTGGCATCTTCTGCAACATCGCACTGCAGAACGATGTCAGAACCCAATTGAGCGGCAAAttcttc > 1:2474899/1‑69 (MQ=255)
gATGCTGGCATCTTCTGCAACATCGCACTGCAGAACGATGTCAGAACCCAATTGAGCGGCAAAttcttc > 1:2078304/1‑69 (MQ=255)
aTCTTCTGCAACATCGCACTGCAGAACGATGTCAGAACCCAATTGAGCGGCAAATTCTTCTACGCGGc < 1:3170132/68‑1 (MQ=255)
aTCTTCTGCAACATCGCACTGCAGAACGATGTCAGAACCCAATTGAGCGGCAAATTCTTCTACGCGGc < 1:3154693/68‑1 (MQ=255)
aTCTTCTGCAACATCGCACTGCAGAACGATGTCAGAACCCAATTGAGCGGCAAATTCTTCTACGCGGc < 1:4855/68‑1 (MQ=255)
aTCTTCTGCAACATCGCACTGCAGAACGATGTCAGAACCCAATTGAGCGGCAAATTCTTCTACGCGGc < 1:2124527/68‑1 (MQ=255)
aTCTTCTGCAACATCGCACTGCAGAACGATGTCAGAACCCAATTGAGCGGCAAATTCTTCTACGCGGc < 1:797009/68‑1 (MQ=255)
aTCTTCTGCAACATCGCACTGCAGAACGATGTCAGAACCCAATTGAGCGGCAAATTCTTCTACGCGGc < 1:1936643/68‑1 (MQ=255)
aTCTTCTGCAACATCGCACTGCAGAACGATGTCAGAACCCAATTGAGCGGCAAATTCTTCTACGCGGc < 1:1054898/68‑1 (MQ=255)
ttctGCAACATCGCACTGCAGAACGATGTCAGAACCCAATTGAGCGGCaaa < 1:2952464/51‑1 (MQ=255)
ttctGCAACATCGCACTGCAGAACGATGTCAGAACCCAATTGAGCGGCAAATTCTTCTACGCGGCCt > 1:1666301/1‑67 (MQ=255)
ttctGCAACATCGCACTGCAGAACGATGTCAGAACCCAATTGAGCGGCAAATTCTTCTACGCGGCCt > 1:1083064/1‑67 (MQ=255)
|
AACCGTCAAATTTCGGCCAAACTTTCCCCAGTTCAGCGAACATGGTGTCGATGCTGGCATCTTCTGCAACATCGCACTGCAGAACGATGTCAGAACCCAATTGAGCGGCAAATTCTTCTACGCGGCCT > minE/889732‑889859
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
