Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F1 I0 R1
|
4577 |
57.4 |
3436498 |
77.7% |
2670158 |
63.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
2,722,403 |
G→A |
65.9% |
intergenic (‑152/‑150) |
crp ← / → yhfA |
DNA‑binding transcriptional dual regulator/conserved hypothetical protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 2,722,403 | 0 | G | A | 65.9%
| 82.8
/ 65.6
| 114 | intergenic (‑152/‑150) | crp/yhfA | DNA‑binding transcriptional dual regulator/conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base G (25/14); new base A (29/46); total (54/60) |
| Fisher's exact test for biased strand distribution p-value = 1.11e-02 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
Only 100 of 115 total aligned reads displayed.
AAGGGGCTATCAACTGTACTGCACGGTAATGTGACGTCCTTTGCATACATGCAGTACATCAATGTATTACTGTAGCATCCTGACTGTTTTAGCATAGCTTTCGCTTTGTGTCTCCTGGTGTCTCGCTTCAG > minE/2722340‑2722470
|
aaGGGGCTATCAACTGTACTGCACGGTAATGTGACGTCCTTTGCATACATGCAGTACATCAATGTAtt < 1:1789184/68‑1 (MQ=255)
ggggCTATCAACTGTACTGCACGGTAATGTGGCGTCCTTTGCATACATGCAGTACATCAATATATTACt < 1:2987332/69‑1 (MQ=255)
ggggCTATCAACTGTACTGCACGGTAATGTGGCGTCCTTTGCATACATGCAGTACATCAATATATTACt < 1:1663509/69‑1 (MQ=255)
ggggCTATCAACTGTACTGCACGGTAATGTGGCGTCCTTTGCATACATGCAGTACATCAATATATTACt < 1:1948900/69‑1 (MQ=255)
ggggCTATCAACTGTACTGCACGGTAATGTGGCGTCCTTTGCATACATGCAGTACATCAATATATTACt < 1:1982234/69‑1 (MQ=255)
ggggCTATCAACTGTACTGCACGGTAATGTGGCGTCCTTTGCATACATGCAGTACATCAATATATTACt < 1:2031030/69‑1 (MQ=255)
ggggCTATCAACTGTACTGCACGGTAATGTGGCGTCCTTTGCATACATGCAGTACATCAATATATTACt < 1:2056944/69‑1 (MQ=255)
ggggCTATCAACTGTACTGCACGGTAATGTGGCGTCCTTTGCATACATGCAGTACATCAATATATTACt < 1:2331643/69‑1 (MQ=255)
ggggCTATCAACTGTACTGCACGGTAATGTGGCGTCCTTTGCATACATGCAGTACATCAATATATTACt < 1:2484715/69‑1 (MQ=255)
ggggCTATCAACTGTACTGCACGGTAATGTGGCGTCCTTTGCATACATGCAGTACATCAATATATTACt < 1:251033/69‑1 (MQ=255)
ggggCTATCAACTGTACTGCACGGTAATGTGGCGTCCTTTGCATACATGCAGTACATCAATATATTACt < 1:2649550/69‑1 (MQ=255)
ggggCTATCAACTGTACTGCACGGTAATGTGGCGTCCTTTGCATACATGCAGTACATCAATATATTACt < 1:2517657/69‑1 (MQ=255)
ggggCTATCAACTGTACTGCACGGTAATGTGGCGTCCTTTGCATACATGCAGTACATCAATATATTACt < 1:3397777/69‑1 (MQ=255)
ggggCTATCAACTGTACTGCACGGTAATGTGGCGTCCTTTGCATACATGCAGTACATCAATATATTACt < 1:358576/69‑1 (MQ=255)
ggggCTATCAACTGTACTGCACGGTAATGTGGCGTCCTTTGCATACATGCAGTACATCAATATATTACt < 1:3713/69‑1 (MQ=255)
ggggCTATCAACTGTACTGCACGGTAATGTGGCGTCCTTTGCATACATGCAGTACATCAATATATTACt < 1:399689/69‑1 (MQ=255)
ggggCTATCAACTGTACTGCACGGTAATGTGGCGTCCTTTGCATACATGCAGTACATCAATATATTACt < 1:551815/69‑1 (MQ=255)
ggggCTATCAACTGTACTGCACGGTAATGTGGCGTCCTTTGCATACATGCAGTACATCAATATATTACt < 1:679161/69‑1 (MQ=255)
ggggCTATCAACTGTACTGCACGGTAATGTGGCGTCCTTTGCATACATGCAGTACATCAATATATTACt < 1:710024/69‑1 (MQ=255)
ggggCTATCAACTGTACTGCACGGTAATGTGGCGTCCTTTGCATACATGCAGTACATCAATATATTACt < 1:935127/69‑1 (MQ=255)
ggggCTATCAACTGTACTGCACGGTAATGTGGCGTCCTTTGCATACATGCAGTACATCAATATATTACt < 1:986841/69‑1 (MQ=255)
ggggCTATCAACTGTACTGCACGGTAATGTGGCGTCCTTTGCATACATGCAGTACATCAATATATTACt < 1:1546612/69‑1 (MQ=255)
aaCTGTACTGCACGGTAATGTGACGTCCTTTGCATACATGCAGTACATCAATGTATTACTGTAGCATc > 1:2563186/1‑68 (MQ=255)
aaCTGTACTGCACGGTAATGTGACGTCCTTTGCATACATGCAGTACATCAATGTATTACTGTAGCATc > 1:3159764/1‑68 (MQ=255)
aaCTGTACTGCACGGTAATGTGACGTCCTTTGCATACATGCAGTACATCAATGTATTACTGTAGCATc > 1:1085225/1‑68 (MQ=255)
cTGTACTGCACGGTAATGTGACGTCCTTTGCATACATGCAGTACATCAATGTATTACTGTAGCATCCt < 1:2773581/68‑1 (MQ=255)
cTGTACTGCACGGTAATGTGACGTCCTTTGCATACATGCAGTACATCAATGTATTACTGTAGCATCCt < 1:2831998/68‑1 (MQ=255)
gTACTGCACGGTAATGTGACGTCCTTTGCATACATGCAGTACATCAATGTATTACTGTAGCATCCTGAc > 1:3357231/1‑69 (MQ=255)
gTACTGCACGGTAATGTGACGTCCTTTGCATACATGCAGTACATCAATGTATTACTGTAGCATCCTGAc > 1:1711441/1‑69 (MQ=255)
gTACTGCACGGTAATGTGACGTCCTTTGCATACATGCAGTACATCAATGTATTACTGTAGCATCCTGAc > 1:2922184/1‑69 (MQ=255)
cTGCACGGTAATGTGACGTCCTTTGCATACATGCAGTACATCAATGTATTACTGTAGCATCCTGACTGt < 1:1598348/69‑1 (MQ=255)
cTGCACGGTAATGTGACGTCCTTTGCATACATGCAGTACATCAATGTATTACTGTAGCATCCTGACTGt < 1:202315/69‑1 (MQ=255)
tGCACGGTAATGTGACGTCCTTTGCATACATGCAGTACATCAATGTATTACTGTAGCATCCTGACTGt < 1:1323737/68‑1 (MQ=255)
ggTAATGTGGCGTCCTTTGCATACATGCAGTACATCAATATATTACTGTAGCATCCTGACTGTTTTa > 1:956484/1‑67 (MQ=255)
ggTAATGTGGCGTCCTTTGCATACATGCAGTACATCAATATATTACTGTAGCATCCTGACTGTTTTa > 1:1740807/1‑67 (MQ=255)
ggTAATGTGGCGTCCTTTGCATACATGCAGTACATCAATATATTACTGTAGCATCCTGACTGTTTTa > 1:2599275/1‑67 (MQ=255)
ggTAATGTGGCGTCCTTTGCATACATGCAGTACATCAATATATTACTGTAGCATCCTGACTGTTTTAg > 1:2883145/1‑68 (MQ=255)
ggTAATGTGGCGTCCTTTGCATACATGCAGTACATCAATATATTACTGTAGCATCCTGACTGTTTTAg > 1:2898545/1‑68 (MQ=255)
ggTAATGTGGCGTCCTTTGCATACATGCAGTACATCAATATATTACTGTAGCATCCTGACTGTTTTAg > 1:3157599/1‑68 (MQ=255)
ggTAATGTGGCGTCCTTTGCATACATGCAGTACATCAATATATTACTGTAGCATCCTGACTGTTTTAg > 1:2752711/1‑68 (MQ=255)
ggTAATGTGGCGTCCTTTGCATACATGCAGTACATCAATATATTACTGTAGCATCCTGACTGTTTTAg > 1:3179071/1‑68 (MQ=255)
ggTAATGTGGCGTCCTTTGCATACATGCAGTACATCAATATATTACTGTAGCATCCTGACTGTTTTAg > 1:3105634/1‑68 (MQ=255)
ggTAATGTGGCGTCCTTTGCATACATGCAGTACATCAATATATTACTGTAGCATCCTGACTGTTTTAg > 1:3092179/1‑68 (MQ=255)
ggTAATGTGGCGTCCTTTGCATACATGCAGTACATCAATATATTACTGTAGCATCCTGACTGTTTTAg > 1:3393633/1‑68 (MQ=255)
ggTAATGTGGCGTCCTTTGCATACATGCAGTACATCAATATATTACTGTAGCATCCTGACTGTTTTAg > 1:2507093/1‑68 (MQ=255)
ggTAATGTGGCGTCCTTTGCATACATGCAGTACATCAATATATTACTGTAGCATCCTGACTGTTTTAg > 1:1416604/1‑68 (MQ=255)
ggTAATGTGGCGTCCTTTGCATACATGCAGTACATCAATATATTACTGTAGCATCCTGACTGTTTTAg > 1:1321802/1‑68 (MQ=255)
ggTAATGTGGCGTCCTTTGCATACATGCAGTACATCAATATATTACTGTAGCATCCTGACTGTTTTAg > 1:1725968/1‑68 (MQ=255)
ggTAATGTGGCGTCCTTTGCATACATGCAGTACATCAATATATTACTGTAGCATCCTGACTGTTTTAg > 1:971212/1‑68 (MQ=255)
ggTAATGTGGCGTCCTTTGCATACATGCAGTACATCAATATATTACTGTAGCATCCTGACTGTTTTAg > 1:748165/1‑68 (MQ=255)
ggTAATGTGGCGTCCTTTGCATACATGCAGTACATCAATATATTACTGTAGCATCCTGACTGTTTTAg > 1:1560250/1‑68 (MQ=255)
ggTAATGTGGCGTCCTTTGCATACATGCAGTACATCAATATATTACTGTAGCATCCTGACTGTTTTAg > 1:630320/1‑68 (MQ=255)
ggTAATGTGGCGTCCTTTGCATACATGCAGTACATCAATATATTACTGTAGCATCCTGACTGTTTTAg > 1:151340/1‑68 (MQ=255)
ggTAATGTGGCGTCCTTTGCATACATGCAGTACATCAATATATTACTGTAGCATCCTGACTGTTTTAg > 1:2110988/1‑68 (MQ=255)
ggTAATGTGGCGTCCTTTGCATACATGCAGTACATCAATATATTACTGTAGCATCCTGACTGTTTTAg > 1:606949/1‑68 (MQ=255)
ggTAATGTGGCGTCCTTTGCATACATGCAGTACATCAATATATTACTGTAGCATCCTGACTGTTTTAg > 1:2157312/1‑68 (MQ=255)
ggTAATGTGGCGTCCTTTGCATACATGCAGTACATCAATATATTACTGTAGCATCCTGACTGTTTTAg > 1:1146762/1‑68 (MQ=255)
ggTAATGTGGCGTCCTTTGCATACATGCAGTACATCAATATATTACTGTAGCATCCTGACTGTTTTAg > 1:2332169/1‑68 (MQ=255)
ggTAATGTGGCGTCCTTTGCATACATGCAGTACATCAATATATTACTGTAGCATCCTGACTGTTTTAg > 1:2459951/1‑68 (MQ=255)
aTGTGACGTCCTTTGCATACATGCAGTACATCAATGTATTACTGTAGCATCCTGACTGt < 1:1093741/59‑1 (MQ=255)
ttGCATACATGCAGTACATCAATATATTACTGTAGCATCCTGACTGTTTTAGCATAGCTTTCGCTTtgt < 1:3292994/69‑1 (MQ=255)
ttGCATACATGCAGTACATCAATATATTACTGTAGCATCCTGACTGTTTTAGCATAGCTTTCGCTTtgt < 1:592761/69‑1 (MQ=255)
ttGCATACATGCAGTACATCAATATATTACTGTAGCATCCTGACTGTTTTAGCATAGCTTTCGCTTtgt < 1:3328509/69‑1 (MQ=255)
ttGCATACATGCAGTACATCAATATATTACTGTAGCATCCTGACTGTTTTAGCATAGCTTTCGCTTtgt < 1:1211838/69‑1 (MQ=255)
ttGCATACATGCAGTACATCAATATATTACTGTAGCATCCTGACTGTTTTAGCATAGCTTTCGCTTtgt < 1:655104/69‑1 (MQ=255)
ttGCATACATGCAGTACATCAATATATTACTGTAGCATCCTGACTGTTTTAGCATAGCTTTCGCTTtgt < 1:3368618/69‑1 (MQ=255)
ttGCATACATGCAGTACATCAATATATTACTGTAGCATCCTGACTGTTTTAGCATAGCTTTCGCTTtgt < 1:1225071/69‑1 (MQ=255)
ttGCATACATGCAGTACATCAATATATTACTGTAGCATCCTGACTGTTTTAGCATAGCTTTCGCTTtgt < 1:1056569/69‑1 (MQ=255)
ttGCATACATGCAGTACATCAATATATTACTGTAGCATCCTGACTGTTTTAGCATAGCTTTCGCTTtgt < 1:1726230/69‑1 (MQ=255)
ttGCATACATGCAGTACATCAATATATTACTGTAGCATCCTGACTGTTTTAGCATAGCTTTCGCTTtgt < 1:1563100/69‑1 (MQ=255)
ttGCATACATGCAGTACATCAATATATTACTGTAGCATCCTGACTGTTTTAGCATAGCTTTCGCTTtgt < 1:2699895/69‑1 (MQ=255)
ttGCATACATGCAGTACATCAATATATTACTGTAGCATCCTGACTGTTTTAGCATAGCTTTCGCTTtgt < 1:2009771/69‑1 (MQ=255)
ttGCATACATGCAGTACATCAATATATTACTGTAGCATCCTGACTGTTTTAGCATAGCTTTCGCTTtgt < 1:2575183/69‑1 (MQ=255)
ttGCATACATGCAGTACATCAATATATTACTGTAGCATCCTGACTGTTTTAGCATAGCTTTCGCTTtgt < 1:1883501/69‑1 (MQ=255)
ttGCATACATGCAGTACATCAATATATTACTGTAGCATCCTGACTGTTTTAGCATAGCTTTCGCTTtgt < 1:1015329/69‑1 (MQ=255)
ttGCATACATGCAGTACATCAATATATTACTGTAGCATCCTGACTGTTTTAGCATAGCTTTCGCTTtgt < 1:2288717/69‑1 (MQ=255)
ttGCATACATGCAGTACATCAATATATTACTGTAGCATCCTGACTGTTTTAGCATAGCTTTCGCTTtgt < 1:1842161/69‑1 (MQ=255)
ttGCATAAATGCAGTACATCAATATATTACTGTAGCATCCTGACTGTTTTAGCATAGCTTTCGCTTtgt < 1:2821145/69‑1 (MQ=255)
cATACATGCAGTACATCAATATATTACTGTAGCATCCTGACTGTTTTAGCATAGCTTTCGCTTtgt < 1:1862710/66‑1 (MQ=255)
aCATGCAGTACATCAATGTATTACTGTAGCATCCTGACTGTTTTAGCATAGCTTTCGCTTTGTGTCTcc > 1:1520481/1‑69 (MQ=255)
aCATGCAGTACATCAATGTATTACTGTAGCATCCTGACTGTTTTAGCATAGCTTTCGCTTTGTGTCTcc > 1:1146626/1‑69 (MQ=255)
aCATGCAGTACATCAATGTATTACTGTAGCATCCTGACTGTTTTAGCATAGCTTTCGCTTTGTGTCTcc > 1:886695/1‑69 (MQ=255)
aCATGCAGTACATCAATGTATTACTGTAGCATCCTGACTGTTTTAGCATAGCTTTCGCTTTGTGTCTcc > 1:934011/1‑69 (MQ=255)
aCATGCAGTACATCAATGTATTACTGTAGCATCCTGACTGTTTTAGCATAGCTTTCGCTTTGTGTCTcc > 1:1057631/1‑69 (MQ=255)
aCATGCAGTACATCAATGTATTACTGTAGCATCCTGACTGTTTTAGCATAGCTTTCGCTTTGTGTCTcc > 1:952160/1‑69 (MQ=255)
aCATGCAGTACATCAATGTATTACTGTAGCATCCTGACTGTTTTAGCATAGCTTTCGCTTTGTGTCTcc > 1:1615336/1‑69 (MQ=255)
aCATGCAGTACATCAATGTATTACTGTAGCATCCTGACTGTTTTAGCATAGCTTTCGCTTTGTGTCTcc > 1:2136394/1‑69 (MQ=255)
aCATGCAGTACATCAATGTATTACTGTAGCATCCTGACTGTTTTAGCATAGCTTTCGCTTTGTGTCTcc > 1:2240476/1‑69 (MQ=255)
aCATGCAGTACATCAATGTATTACTGTAGCATCCTGACTGTTTTAGCATAGCTTTCGCTTTGTGTCTcc > 1:544161/1‑69 (MQ=255)
aCATGCAGTACATCAATGTATTACTGTAGCATCCTGACTGTTTTAGCATAGCTTTCGCTTTGTGTCTcc > 1:1375842/1‑69 (MQ=255)
aCATGCAGTACATCAATGTATTACTGTAGCATCCTGACTGTTTTAGCATAGCTTTCGCTTTGTGTCTcc > 1:3136168/1‑69 (MQ=255)
aCATGCAGTACATCAATGTATTACTGTAGCATCCTGACTGTTTTAGCATAGCTTTCGCTTTGTGTCTcc > 1:2991858/1‑69 (MQ=255)
cATGCAGTACATCAATGTATTACTGTAGCATCCTGACTGTTTTAGCATAGCTTTCGCTTTGTGtctc < 1:2390147/67‑1 (MQ=255)
tGCAGTACATCAATGTATTACTGTAGCATCCTGACTGTTTTAGCATAGCTTTCGCTTTGTGTCTCCTgg > 1:227808/1‑69 (MQ=255)
tGCAGTACATCAATGTATTACTGTAGCATCCTGACTGTTTTAGCATAGCTTTCGCTTTGTGTCTCCTgg > 1:2757681/1‑69 (MQ=255)
cAGTACATCAATGTATTACTGTAGCATCCTGACTGTTTTAGCATAGCTTTCGCTTTGTGTCTCCTGgtg > 1:885937/1‑69 (MQ=255)
cAGTACATCAATGTATTACTGTAGCATCCTGACTGTTTTAGCATAGCTTTCGCTTTGTGTCTCCTGgtg > 1:2533752/1‑69 (MQ=255)
tGTATTACTGTAGCATCCTGACTGTTTTAGCATAGCTTTCGCTTTGTGTCTCCTGGTGTCTCGCTTCAg < 1:3078869/69‑1 (MQ=255)
tGTATTACTGTAGCATCCTGACTGTTTTAGCATAGCTTTCGCTTTGTGTCTCCTGGTGTCTCGCTTCAg < 1:1364334/69‑1 (MQ=255)
gTATTACTGTAGCATCCTGACTGTTTTAGCATAGCTTTCGCTTTGTGTCTCCTGGTGTCTCGCTTCAg < 1:1481875/68‑1 (MQ=255)
|
AAGGGGCTATCAACTGTACTGCACGGTAATGTGACGTCCTTTGCATACATGCAGTACATCAATGTATTACTGTAGCATCCTGACTGTTTTAGCATAGCTTTCGCTTTGTGTCTCCTGGTGTCTCGCTTCAG > minE/2722340‑2722470
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
