Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F1 I0 R1
|
4577 |
57.4 |
3436498 |
77.7% |
2670158 |
63.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
2,726,336 |
G→A |
48.9% |
Y249Y (TAC→TAT) |
yheS ← |
fused predicted transporter subunits and ATP‑binding components of ABC superfamily |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 2,726,336 | 0 | G | A | 48.9%
| ‑2.2
/ 39.8
| 47 | Y249Y (TAC→TAT) | yheS | fused predicted transporter subunits and ATP‑binding components of ABC superfamily |
| Reads supporting (aligned to +/- strand): ref base G (3/21); new base A (6/17); total (9/38) |
| Fisher's exact test for biased strand distribution p-value = 2.86e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
GCTTTGGCACGGAAACGGTCGATATAACTTTGCAGATGCGCTACGCGTTCCTGCTGGCTTTCGTACATCGCTTGTTGCTGCGCCAGACGGGTGGCGCGCTGTACTTCAAACGAACTGTA > minE/2726274‑2726392
|
gCTTTGGCACGGAAACGGTCGATATAACTTTGCAGATGCGCTACGCGTTCCTGCTGGCTTTCGTACATc > 1:1330587/1‑69 (MQ=255)
gCTTTGGCACGGAAACGGTCGATATAACTTTGCAGATGCGCTACGCGTTCCTGCTGGCTTTCGTACATc > 1:3170867/1‑69 (MQ=255)
gCTTTGGCACGGAAACGGTCGATATAACTTTGCAGATGCGCTACGCGGTCCTGCTGGCTTTCGTACATc > 1:1960098/1‑69 (MQ=255)
ggAAACGGTCGATATAACTTTGCAAATGCGCCACGCGTTCCTGCTGGGTTTCATACATCGCTTGTtgct > 1:698749/1‑69 (MQ=255)
ggAAACGGTCGATATAACTTTGCAAATGCGCCACGCGTTCCTGCTGGCTTTCATACATCGCttgttg > 1:3048808/1‑67 (MQ=255)
ggAAACGGTCGATATAACTTTGCAAATGCGCCACGCGTTCCTGCTGGCTTTCATACATCGCTTGTtgct > 1:1313723/1‑69 (MQ=255)
ggAAACGGTCGATATAACTTTGCAAATGCGCCACGCGTTCCTGCTGGCTTTCATACATCGCTTGTtgct > 1:3172843/1‑69 (MQ=255)
ggAAACGGTCGATATAACTTTGCAAATGCGCCACGCGTTCCTGCTGGCTTTCATACATCGCTTGTtgct > 1:215335/1‑69 (MQ=255)
ggAAACGGTCGATATAACTTTGCAAATGCGCCACGCGTTCCTGCTGGCTTTCATACATCGCTTGTtgct > 1:2252193/1‑69 (MQ=255)
aGATGCGCTACGCGTTCCTGCTGGCTTTCGTACATCGCTTGTTGCTGCGCCAGACGGGTGGCGCGCTg < 1:3050790/68‑1 (MQ=255)
aGATGCGCTACGCGTTCCTGCTGGCTTTCGTACATCGCTTGTTGCTGCGCCAGACGGGTGGCGCGCTg < 1:954889/68‑1 (MQ=255)
aGATGCGCTACGCGTTCCTGCTGGCTTTCGTACATCGCTTGTTGCTGCGCCAGACGGGTGGCGCGCTg < 1:336798/68‑1 (MQ=255)
aGATGCGCTACGCGTTCCTGCTGGCTTTCGTACATCGCTTGTTGCTGCGCCAGACGGGTGGCGCGCTg < 1:3170832/68‑1 (MQ=255)
aGATGCGCTACGCGTTCCTGCTGGCTTTCGTACATCGCTTGTTGCTGCGCCAGACGGGTGGCGCGCTg < 1:2989531/68‑1 (MQ=255)
aGATGCGCTACGCGTTCCTGCTGGCTTTCGTACATCGCTTGTTGCTGCGCCAGACGGGTGGCGCGCTg < 1:2556849/68‑1 (MQ=255)
gATGCGCTACGCGTTCCTGCTGGCTTTCGTACATCGCTTGTTGCTGCGCCAGACGGGTGGCGCGCTg < 1:3321919/67‑1 (MQ=255)
ccTGCTGGCTTTCGTACATCGCTTGTTGCTGCGCCAGACGGGTGGCGCTCTGTACTTCAAACGAACTGt < 1:2891604/69‑1 (MQ=255)
ccTGCTGGCTTTCGTACATCGCTTGTTGCTGCGCCAGACGGGTGGCGCGCTGTACTTCAAACGAACTGt < 1:959026/69‑1 (MQ=255)
ccTGCTGGCTTTCGTACATCGCTTGTTGCTGCGCCAGACGGGTGGCGCGCTGTACTTCAAACGAACTGt < 1:88076/69‑1 (MQ=255)
ccTGCTGGCTTTCGTACATCGCTTGTTGCTGCGCCAGACGGGTGGCGCGCTGTACTTCAAACGAACTGt < 1:60838/69‑1 (MQ=255)
ccTGCTGGCTTTCGTACATCGCTTGTTGCTGCGCCAGACGGGTGGCGCGCTGTACTTCAAACGAACTGt < 1:372950/69‑1 (MQ=255)
ccTGCTGGCTTTCGTACATCGCTTGTTGCTGCGCCAGACGGGTGGCGCGCTGTACTTCAAACGAACTGt < 1:521386/69‑1 (MQ=255)
ccTGCTGGCTTTCGTACATCGCTTGTTGCTGCGCCAGACGGGTGGCGCGCTGTACTTCAAACGAACTGt < 1:369040/69‑1 (MQ=255)
ctgctgGCTTTCGTACATCGCTTGTTGCTGCGCCAGACGGGTGGCGCGCTGTACTTCAAACGAACTGTa < 1:2018835/69‑1 (MQ=255)
tgctgGCTTTCGTACATCGCTTGTTGCTGCGCCAGACGGGTGGCGCGCTGTACTTCAAACGAACTGt < 1:410559/67‑1 (MQ=255)
tgctgGCTTTCGTACATCGCTTGTTGCTGCGCCAGACGGGTGGCGCGCTGTACTTCAAACGAACTGTa < 1:41464/68‑1 (MQ=255)
tgctgGCTTTCGTACATCGCTTGTTGCTGCGCCAGACGGGTGGCGCGCTGTACTTCAAACGAACTGTa < 1:1514849/68‑1 (MQ=255)
tgctgGCTTTCGTACATCGCTTGTTGCTGCGCCAGACGGGTGGCGCGCTGTACTTCAAACGAACTGTa < 1:1261972/68‑1 (MQ=255)
tgctgGCTTTCGTACATCGCTTGTTGCTGCGCCAGACGGGTGGCGCGCTGTACTTCAAACGAACTGTa < 1:2325775/68‑1 (MQ=255)
gctgGCTTTCGTACATCGCTTGTTGCTGCGCCAGACGGGTGGCGCGCTGTACTTCAAACGAACTGTa < 1:1625377/67‑1 (MQ=255)
tgGCTTTCATACATCGCTTGTTGCTGCGCCAGACgg < 1:602231/36‑1 (MQ=255)
tgGCTTTCATACATCGCTTGTTGCTGCGCCAGACgg < 1:469091/36‑1 (MQ=255)
tgGCTTTCATACATCGCTTGTTGCTGCGCCAGACgg < 1:513897/36‑1 (MQ=255)
tgGCTTTCATACATCGCTTGTTGCTGCGCCAGACgg < 1:1614167/36‑1 (MQ=255)
tgGCTTTCATACATCGCTTGTTGCTGCGCCAGACgg < 1:1357886/36‑1 (MQ=255)
tgGCTTTCATACATCGCTTGTTGCTGCGCCAGACgg < 1:978163/36‑1 (MQ=255)
tgGCTTTCATACATCGCTTGTTGCTGCGCCAGACgg < 1:3333083/36‑1 (MQ=255)
tgGCTTTCATACATCGCTTGTTGCTGCGCCAGACgg < 1:1661036/36‑1 (MQ=255)
tgGCTTTCATACATCGCTTGTTGCTGCGCCAGACgg < 1:2001414/36‑1 (MQ=255)
tgGCTTTCATACATCGCTTGTTGCTGCGCCAGACgg < 1:3164835/36‑1 (MQ=255)
tgGCTTTCATACATCGCTTGTTGCTGCGCCAGACgg < 1:3116960/36‑1 (MQ=255)
tgGCTTTCATACATCGCTTGTTGCTGCGCCAGACgg < 1:2232293/36‑1 (MQ=255)
tgGCTTTCATACATCGCTTGTTGCTGCGCCAGACgg < 1:2974180/36‑1 (MQ=255)
tgGCTTTCATACATCGCTTGTTGCTGCGCCAGACgg < 1:2813563/36‑1 (MQ=255)
tgGCTTTCATACATCGCTTGTTGCTGCGCCAGACgg < 1:2246694/36‑1 (MQ=255)
tgGCTTTCATACATCGCTTGTTGCTGCGCCAGACgg < 1:2535485/36‑1 (MQ=255)
ggCTTTCATACATCGATTGTTGCTGCGCCAGACgg < 1:846806/35‑1 (MQ=37)
|
GCTTTGGCACGGAAACGGTCGATATAACTTTGCAGATGCGCTACGCGTTCCTGCTGGCTTTCGTACATCGCTTGTTGCTGCGCCAGACGGGTGGCGCGCTGTACTTCAAACGAACTGTA > minE/2726274‑2726392
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
