Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A3 F50 I3 R1 283 58.3 1819865 98.8% 1798026 150.1

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,266,451 A→G T120A (ACC→GCC)  prmC → RF‑1 and RF‑2 N5‑glutamine methyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,266,4510AG100.0% 151.0 / NA 44T120A (ACC→GCC) prmCRF‑1 and RF‑2 N5‑glutamine methyltransferase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (18/26);  total (18/26)

TAACCGGGGTGCGAGAATTCTGGTCGTTGCCGTTATTTGTTTCGCCAGCGACCTTAATTCCGCGCCCGGATACGGAGTGTCTGGTGGAGCAGGCACTGGCGCGGTTGCCTGAACAACCTTGCCGTATTCTCGATCTCGGGACGGGTACCGGGGCGATTGCGCTTGCGCTGGCTAGCGAGCGCCCGGACTGCGAAATTATCGCTGTAGATCGTATGCCTGATGCTGTCTCCCTGGCACAACGTAATGCCCAGCATCTGGCGATCAAAAATATCCACATTCTGCAAAGCGACTG  >  NC_000913/1266305‑1266596
                                                                                                                                                  |                                                                                                                                                 
tAACCGGGGTGCGAGAATTCTGGTCGTTGCCGTTATTTGTTTCGCCAGCGACCTTAATTCCGCGCCCGGATACGGAGTGTCTGGTGGAGCAGGCACTGGCGCGGTTGCCTGAACAACCTTGCCGTATTCTCGATCTCGGGACGGGTGCCg                                                                                                                                                <  2:121494/150‑1 (MQ=255)
     ggggTGCGAGAATTCTGGTCGTTGCCGTTATTTGTTTCGCCAGCGACCTTAATTCCGCGCCCGGATACGGAGTGTCTGGTGGAGCAGGCACTGGCGCGGTTGCCTGAACAACCTTGCCATATTCTCGATCTCGGGACGGGTGCCGGGGCg                                                                                                                                           >  1:575413/1‑150 (MQ=255)
      gggTGCGAGAATTCTTGGCGTTGCCGTTATTTGTTTCGCCAGCGGCCTTAATTCCGGGCCCGGATACGGGGTTTCTGGTGGAGGAGGCACTGGCGGGGTTGGCTGAACAACCTTGCCCTTTTCCCGATCCCGGGACGGGTGCCGGGGCGAt                                                                                                                                         <  2:628119/151‑1 (MQ=255)
             agaATTCTGGTCGTTGCCGTTATTTGTTTCGCCAGCGACCTTAATTCCGCGCCCGGATACGGAGTGTCTGGTGGAGCAGGCACTGGCGCGGTTGCCTGAACAACCTTGCCGTATTCTCGATCTCGGGACGGGTGCCGGGGCGAttgcgctt                                                                                                                                  <  2:900642/151‑1 (MQ=255)
             agaATTCTGGTCGTTGCCGTTATTTGTTTCGCCAGCGACCTTAATTCCGCGCCCGGATACGGAGTGTCTGGTGGAGCAGGCACTGGCGCGGTTGCCTGAACAACCTTGCCGTATTCTCGATCTCGGGACGGGTGCCGGGGCGAttgcgctt                                                                                                                                  >  2:511438/1‑151 (MQ=255)
             agaATTCTGGTCGTTGCCGTTATTTGTTTCGCCAGCGACCTTAACTCCGCGCCCGCATGCGGAGAGTGTGGAGGAGCAGGCACTGGCGCGCGTGCCGGAACAACCGTGCCGTCTTCTCGCTCTCGGGGTGGGGGCCGGGGGGAttgcgctt                                                                                                                                  >  2:248214/1‑151 (MQ=255)
                     ggTCGTTGCCGTTATTTGTTTCGCCAGCGACCTTAATTCCGCGCCCGGATACGGAGTGTCTGGTGGAGCAGGCACTGGCGCGGTTGCCTGAACAACCTTGCCGTATTCTCGATCTCGGGACGGGTGCCGGGGCGATTGCGCTTGCGCTGGc                                                                                                                          <  1:43004/151‑1 (MQ=255)
                        cGTTGCCGTTATTTGTTTCGCCAGCGACCTTAATTCCGCGCCCGGATACGGAGTGTCTGGTGGAGCAGGCACTGGCGCGGTTGCCTGAACAACCTTGCCGTATTCTCGATCTCGGGACGGGTGCCGGGGCGATTGCGCTTGCGCTGGCTag                                                                                                                       <  2:846307/151‑1 (MQ=255)
                                    ttGTTTCGCCAGCGACCTTAATTCCGCGCCCGGATACGGAGTGTCTGGTGGAGCAGGCACTGGCGCGGTTGCCTGAACAACCTTGCCGTATTCTCGATCTCGGGACGGGTGCCGGGGCGATTGCGCTTGCGCTGGCTAGCGAGCGCCCgg                                                                                                            >  2:453431/1‑150 (MQ=255)
                                        ttCGCCAGCGACCTTAATTCCGCGCCCGGATACGGAGTGTCTGGTGGAGCAGGCACTGGCGCGGTTGCCTGAACAACCTTGCCGTATTCTCGATCTCGGGACGGGTGCCGGGGCGATTGCGCTTGCGCTGGCTAGCGAGCGCCCGGACTg                                                                                                        <  2:438700/150‑1 (MQ=255)
                                        ttCGCCAGCGACCTTAATTCCGCGCCCGGATACGGAGTGTCTGGTGGAGCAGGCACTGGCGCGGTTGCCTGAACAACCTTGCCGTATTCTCGATCTCGGGACGGGTGCCGGGGCGATTGCGCTTGCGCTGGCTAGCGAGCGCCCGGACTg                                                                                                        <  2:909890/150‑1 (MQ=255)
                                              aGCGACCTTAATTCCGCGCCCGGATACGGAGTGTCTGGTGGAGCAGGCACTGGCGCGGTTGCCTGAACAACCTTGCCGTATTCTCGATCTCGGGACGGGTGCCGGGGCGATTGCGCTTGCGCTGGCTAGCGAGCGCCCGGACTGCGAAAtt                                                                                                 <  2:579419/151‑1 (MQ=255)
                                                 gACCTTAATTCCGCGCCCGGATACGGAGTGTCTGGTGGAGCAGGCACTGGCGCGGTTGCCTGAACAACCTTGCCGTATTCTCGATCTCGGGACGGGTGCCGGGGCGATTGCGCTTGCGCTGGCTAGCGAGCGCCCGGACTGCGAAATTATc                                                                                              >  2:470200/1‑151 (MQ=255)
                                                 gACCTTAATTCCGCGCCCGGATACGGAGTGTCTGGTGGAGCAGGCACTGGCGCGGTTGCCTGAACAACCTTGCCGTATTCTCGATCTCGGGACGGGTGCCGGGGCGATTGCGCTTGCGCTGGCTAGCGAGCGCCCGGACTGCGAAATTATc                                                                                              >  1:178680/1‑151 (MQ=255)
                                                            cgcgCCCGGATACGGAGTGTCTGGTGGAGCAGGCACTGGCGCGGTTGCCTGAACAACCTTGCCGTATTCTCGATCTCGGGACGGGTGCCGGGGCGATTGCGCTTGCGCTGGCTAGCGAGCGCCCGGACTGCGAAATTATCGCTGt                                                                                         >  2:53546/1‑145 (MQ=255)
                                                            cgcgCCCGGATACGGAGTGTCTGGTGGAGCAGGCACTGGCGCGGTTGCCTGAACAACCTTGCCGTATTCTCGATCTCGGGACGGGTGCCGGGGCGATTGCGCTTGCGCTGGCTAGCGAGCGCCCGGACTGCGAAATTATCGCTGTAGATCg                                                                                   <  1:560800/151‑1 (MQ=255)
                                                                   ggATACGGAGTGTCTGGTGGAGCAGGCACTGGCGCGGTTGCCTGAACAACCTTGCCGTATTCTCGATCTCGGGACGGGTGCCGGGGCGATTGCGCTTGCGCTGGCTAGCGAGCGCCCGGACTGCGAAATTATCGCTGTAGATCGTATGCCt                                                                            >  1:129357/1‑151 (MQ=255)
                                                                    gATACGGAGTGTCTGGTGGAGCAGGCACTGGCGCGGTTGCCTGAACAACCTTGCCGTATTCTCGATCTCGGGACGGGTGCCGGGGCGATTGCGCTTGCGCTGGCTAGCGAGCGCCCGGACTGCGAAATTATCGCTGTAGATCGTATGCCTg                                                                           >  1:369250/1‑151 (MQ=255)
                                                                      tACGGAGTGTCTGGTGGAGCAGGCACTGGCGCGGTTGCCTGAACAACCTTGCCGTATTCTCGATCTCGGGACGGGTGCCGGGGCGATTGCGCTTGCGCTGGCTAGCGAGCGCCCGGACTGCGAAATTATCGCTGTAGATCGTATGcc                                                                             >  2:221445/1‑147 (MQ=255)
                                                                         ggAGTGTCTGGTGGAGCAGGCACTGGCGCGGTTGCCTGAACAACCTTGCCGTATTCTCGATCTCGGGACGGGTGCCGGGGCGATTGCGCTTGCGCTGGCTAGCGAGCGCCCGGACTGCGAAATTATCGCTGTAGATCGTATGCCTGATGCt                                                                      <  1:323004/151‑1 (MQ=255)
                                                                              gtCTGGTGGAGCAGGCACTGGCGCGGTTGCCTGAACAACCTTGCCGTATTCTCGATCTCGGGACGGGTGCCGGGGCGATTGCGCTTGCGCTGGCTAGCGAGCGCCCGGACTGCGAAATTATCGCTGTAGATCGTATGCCTGATGCTGtctc                                                                 <  1:768690/151‑1 (MQ=255)
                                                                                  ggtggAGCAGGCACTGGCGCGGTTGCCTGAACAACCTTGCCGTATTCTCGATCTCGGGACGGGTGCCGGGGCGATTGCGCTTGCGCTGGCTAGCGAGCGCCCGGACTGCGAAATTATCGCTGTAGATCGTATGCCTGATGCTGTCTccc                                                               <  1:567042/149‑1 (MQ=255)
                                                                                     ggAGCAGGCACTGGCGCGGTTGCCTGAACAACCTTGCCGTATTCTCGATCTCGGGACGGGTGCCGGGGCGATTGCGCTTGCGCTGGCTAGCGAGCGCCCGGACTGCGAAATTATCGCTGTAGATCGTATGCCTGATGCTGTCTCCCTGGc                                                           >  2:731476/1‑150 (MQ=255)
                                                                                         cAGGCACTGGCGCGGTTGCCTGAACAACCTTGCCGTATTCTCGATCTCGGGACGGGTGCCGGGGCGATTGCGCTTGCGCTGGCTAGCGAGCGCCCGGACTGCGAAATTATCGCTGTAGATCGTATGCCTGATGCTGTCTCCCTGGCACAAc                                                      >  2:564322/1‑151 (MQ=255)
                                                                                                  gcgcGGTTGCCTGAACAACCTTGCCGTATTCTCGATCTCGGGACGGGTGCCGGGGCGATTGCGCTTGCGCTGGCTAGCGAGCGCCCGGACTGCGAAATTATCGCTGTAGATCGTATGCCTGATGCTGTCTCCCTGGCACAACGTAATGccc                                             <  2:172773/151‑1 (MQ=255)
                                                                                                       gTTGCCTGAACAACCTTGCCGTATTCTCGATCTCGGGACGGGTGCCGGGGCGATTGCGCTTGCGCTGGCTAGCGAGCGCCCGGACTGCGAAATTATCGCTGTAGATCGTATGCCTGATGCTGTCTCCCTGGCACAACGTAATGCCCAGCAt                                        >  1:351625/1‑151 (MQ=255)
                                                                                                            cTGAACAACCTTGCCGTATTCTCGATCTCGGGACGGGTGCCGGGGCGATTGCGCTTGCGCTGGCTAGCGAGCGCCCGGACTGCGAAATTATCGCTGTAGATCGTATGCCTGATGCTGTCTCCCTGGCACAACGTAATGCCCAGCATCTgg                                    >  2:508256/1‑150 (MQ=255)
                                                                                                             tGAACAACCTTGCCGTATTCTCGATCTCGGGACGGGTGCCGGGGCGATTGCGCTTGCGCTGGCTAGCGAGCGCCCGGACTGCGAAATTATCGCTGTAGATCGTATGCCTGATGCTGTCTCCCTGGCACAACGTAATGCCCAGCATCTGGCg                                  >  2:407118/1‑151 (MQ=255)
                                                                                                              gAACAACCTTGCCGTATTCTCGATCTCGGGACGGGTGCCGGGGCGATTGCGCTTGCGCTGGCTAGCGAGCGCCCGGACTGCGAAATTATCGCTGTAGATCGTATGCCTGATGCTGTCTCCCTGGCACAACGTAATGCCCAGCATCTGGCg                                  <  2:200459/150‑1 (MQ=255)
                                                                                                              gAACAACCTTGCCGTATTCTCGATCTCGGGACGGGTGCCGGGGCGATTGCGCTTGCGCTGGCTAGCGAGCGCCCGGACTGCGAAATTATCGCTGTAGATCGTATGCCTGATGCTGTCTCCCTGGCACAACGTAATGCCCAGCATCTGGCGa                                 <  1:807864/151‑1 (MQ=255)
                                                                                                                       tGCCGTATTCTCGATCTCGGGACGGGTGCCGGGGCGATTGCGCTTGCGCTGGCTAGCGAGCGCCCGGACTGCGAAATTATCGCTGTAGATCGTATGCCTGATGCTGTCTCCCTGGCACAACGTAATGCCCAGCATCTGGCGATCaaaaa                          <  2:532848/149‑1 (MQ=255)
                                                                                                                       tGCCGTATTCTCGATCTCGGGACGGGTGCCGGGGCGATTGCGCTTGCGCTGGCTAGCGAGCGCCCGGACTGCGAAATTATCGCTGTAGATCGTATGCCTGATGCTGTCTCCCTGGCACAACGTAATGCCCAGCATCTGGCGATCAAAAat                         <  1:161391/150‑1 (MQ=255)
                                                                                                                        gCCGTATTCTCGATCTCGGGACGGGTGCCGGGGCGATTGCGCTTGCGCTGGCTAGCGAGCGCCCGGACTGCGAAATTATCGCTGTAGATCGTATGCCTGATGCTGTCTCCCTGGCACAACGTAATGCCCAGCATCTGGCGATCAAAAatat                       <  2:526069/151‑1 (MQ=255)
                                                                                                                             aTTCTCGATCTCGGGACGGGTGCCGGGGCGATTGCGCTTGCGCTGGCTAGCGAGCGCCCGGACTGCGAAATTATCGCTGTAGATCGTATGCCTGATGCTGTCTCCCTGGCACAACGTAATGCCCAGCATCTGGCGATCAAAAATATCcac                   <  2:324886/150‑1 (MQ=255)
                                                                                                                                ctcGATCTCGGGACGGGTGCCGGGGCGATTGCGCTTGCGCTGGCTAGCGAGCGCCCGGACTGCGAAATTATCGCTGTAGATCGTATGCCTGATGCTGTCTCCCTGGCACAACGTAATGCCCAGCATCTGGCGATCAAAAATATCCACATTc               >  2:333844/1‑151 (MQ=255)
                                                                                                                                  cGATCTCGGGACGGGTGCCGGGGCGATTGCGCTTGCGCTGGCTAGCGAGCGCCCGGACTGCGAAATTATCGCTGTAGATCGTATGCCTGATGCTGTCTCCCTGGCACAACGTAATGCCCAGCATCTGGCGATCAAAAATATCCACAtt                <  1:810773/148‑1 (MQ=255)
                                                                                                                                  cGATCTCGGGACGGGTGCCGGGGCGATTGCGCTTGCGCTGGCTAGCGAGCGCCCGGACTGCGAAATTATCGCTGTAGATCGTATGCCTGATGCTGTCTCCCTGGCACAACGTAATGCCCAGCATCTGGCGATCAAAAATATCCACAtt                <  1:453478/148‑1 (MQ=255)
                                                                                                                                        cGGGACGGGTGCCGGGGCGATTGCGCTTGCGCTGGCTAGCGAGCGCCCGGACTGCGAAATTATCGCTGTAGATCGTATGCCTGATGCTGTCTCCCTGGCACAACGTAATGCCCAGCATCTGGCGATCAAAAATATCCACATTCTg             <  2:563896/145‑1 (MQ=255)
                                                                                                                                        cGGGACGGGTGCCGGGGCGATTGCGCTTGCGCTGGCTAGCGAGCGCCCGGACTGCGAAATTATCGCTGTAGATCGTATGCCTGATGCTGTCTCCCTGGCACAACGTAATGCCCAGCATCTGGCGATCAAAAATATCCACATTCTGCaa          <  2:306230/148‑1 (MQ=255)
                                                                                                                                        cGGGACGGGTGCCGGGGCGATTGCGCTTGCGCTGGCTAGCGAGCGCCCGGACTGCGAAATTATCGCTGTAGATCGTATGCCTGATGCTGTCTCCCTGGCACAACGTAATGCCCAGCATCTGGCGATCAAAAATATCCACATTCTGCAAAg        <  2:515594/150‑1 (MQ=255)
                                                                                                                                         gggACGGGTGCCGGGGCGATTGCGCTTGCGCTGGCTAGCGAGCGCCCGGACTGCGAAATTATCGCTGTAGATCGTATGCCTGATGCTGTCTCCCTGGCACAACGTAATGCCCAGCATCTGGCGATCAAAAATATCCACATTCTg             >  2:648217/1‑144 (MQ=255)
                                                                                                                                         gggACGGGTGCCGGGGCGATTGCGCTTGCGCTGGCTAGCGAGCGCCCGGACTGCGAAATTATCGCTGTAGATCGTATGCCTGATGCTGTCTCCCTGGCACAACGTAATGCCCAGCATCTGGCGATCAAAAATATCCACATTCTg             <  1:648266/144‑1 (MQ=255)
                                                                                                                                         gggACGGGTGCCGGGGCGATTGCGCTTGCGCTGGCTAGCGAGCGCCCGGACTGCGAAATTATCGCTGTAGATCGTATGCCTGATGCTGTCTCCCTGGCACAACGTAATGCCCAGCATCTGGCGATCAAAAATATCCACATTCTGCAAAGCg      >  1:455672/1‑151 (MQ=255)
                                                                                                                                             cGGGTGCCGGGGCGATTGCGCTTGCGCTGGCTAGCGAGCGCCCGGACTGCGAAATTATCGCTGTAGATCGTATGCCTGATGCTGTCTCCCTGGCACAACGTAATGCCCAGCATCTGGCGATCAAAAATATCCACATTCTGCAAAGCGACTg  <  1:615631/151‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                 
TAACCGGGGTGCGAGAATTCTGGTCGTTGCCGTTATTTGTTTCGCCAGCGACCTTAATTCCGCGCCCGGATACGGAGTGTCTGGTGGAGCAGGCACTGGCGCGGTTGCCTGAACAACCTTGCCGTATTCTCGATCTCGGGACGGGTACCGGGGCGATTGCGCTTGCGCTGGCTAGCGAGCGCCCGGACTGCGAAATTATCGCTGTAGATCGTATGCCTGATGCTGTCTCCCTGGCACAACGTAATGCCCAGCATCTGGCGATCAAAAATATCCACATTCTGCAAAGCGACTG  >  NC_000913/1266305‑1266596

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A