Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A3 F50 I3 R1	283	58.3	1819865	98.8%	1798026	150.1

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,678,816	T→C	E184E (GAA→GAG)	yphF ←	putative sugar ABC transporter periplasmic binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,678,816	0	T	C	100.0%	213.3 / NA	58	E184E (GAA→GAG)	yphF	putative sugar ABC transporter periplasmic binding protein
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (25/33); total (25/33)

GAGTGTCGCACCGCCCGATTCCCCCATAATGGCGTTGAGATCCGGCGTGGAGATAATTAGTTTTTCACCAACGGAAATCGCTTTATCTAAAACAGTCCCTTCCTGATTAGCGACAATTTGCGCGCCGGGAACGCGGGATTTTAAAACTTCTTCAAATCCTTTACGTCGCTGCACACAAACTTCAAAGGCTTCGCAATTGATGACGGCAATTTTCGGCTGGTCAATTTTATTGGCAATAAAATAATCAGCGGCAGCGTTACCCAGTTTTTTACCAAATTCCAGCGGATCG  >  NC_000913/2678669‑2678957

gAGTGTCGCACCGCCCGATTCCCCCATAATGGCGTTGAGATCCGGCGTGGAGATAATTAGTTTTTCACCAACGGAAATCGCTTTATCTAAAACAGTCCCTTCCTGATTAGCGACAATTTGCGCGCCGGGAACGCGGGATTTTAAAAcctct                                                                                                                                            >  1:647485/1‑151 (MQ=255)

 aGTGTCGCACCGCCCGATTCCCCCATAATGGCGTTGAGATCCGGCGTGGAGATAATTAGTTTTTCACCAACGGAAATCGCTTTATCTAAAACAGTCCCTTCCTGATTAGCGACAATTTGCGCGCCGGGAACGCGGGATTTTAAAAcctctt                                                                                                                                           <  1:164658/151‑1 (MQ=255)

    gtCGCACCGCCCGATTCCCCCATAATGGCGTTGAGATCCGGCGTGGAGATAATTAGTTTTTCACCAACGGAAATCGCTTTATCTAAAACAGTCCCTTCCTGATTAGCGACAATTTGCGCGCCGGGAACGCGGGATTTTAAAACCTCTTCa                                                                                                                                         <  2:561236/150‑1 (MQ=255)

         aCCGCCCGATTCCCCCATAATGGCGTTGAGATCCGGCGTGGAGATAATTAGTTTTTCACCAACGGAAATCGCTTTATCTAAAACAGTCCCTTCCTGATTAGCGACAATTTGCGCGCCGGGAACGCGGGATTTTAAAACCTCTTCAAATCCt                                                                                                                                   >  2:77925/1‑151 (MQ=255)

            gCCCGATTCCCCCATAATGGCGTTGAGATCCGGCGTGGAGATAATTAGTTTTTCACCAACGGAAATCGCTTTATCTAAAACAGTCCCTTCCTGATTAGCGACAATTTGCGCGCCGGGAACGCGGGATTTTAAAACCTCTTCAAATCCttt                                                                                                                                 >  2:563305/1‑150 (MQ=255)

            gCCCGATTCCCCCATAATGGCGTTGAGATCCGGCGTGGAGATAATTAGTTTTTCACCAACGGAAATCGCTTTATCTAAAACAGTCCCTTCCTGATTAGCGACAATTTGCGCGCCGGGAACGCGGGATTTTAAAACCTCTTCAAATCCttt                                                                                                                                 >  1:708795/1‑150 (MQ=255)

            gCCCGATTCCCCCATAATGGCGTTGAGATCCGGCGTGGAGATAATTAGTTTTTCACCAACGGAAATCGCTTTATCTAAAACAGTCCCTTCCTGATTAGCGACAATTTGCGCGCCGGGAACGCGGGATTTTAAAACCTCTTCAAATCCTTTa                                                                                                                                <  1:251183/151‑1 (MQ=255)

                gATTCCCCCATAATGGCGTTGAGATCCGGCGTGGAGATAATTAGTTTTTCACCAACGGAAATCGCTTTATCTAAAACAGTCCCTTCCTGATTAGCGACAATTTGCGCGCCGGGAACGCGGGATTTTAAAACCTCTTCAAATCCTTTACGTc                                                                                                                            <  2:282149/151‑1 (MQ=255)

                gATTCCCCCATAATGGCGTTGAGATCCGGCGTGGAGATAATTAGTTTTTCACCAACGGAAATCGCTTTATCTAAAACAGTCCCTTCCTGATTAGCGACAATTTGCGCGCCGGGAACGCGGGATTTTAAAACCTCTTCAAATCCTTTACGTc                                                                                                                            >  2:634680/1‑151 (MQ=255)

                 aTTCCCCCATAATGGCGTTGAGATCCGGCGTGGAGATAATTAGTTTTTCACCAACGGAAATCGCTTTATCTAAAACAGTCCCTTCCTGATTAGCGACAATTTGCGCGCCGGGAACGCGGGATTTTAAAACCTCTTCAAATCCTTTACGTCg                                                                                                                           <  2:595036/151‑1 (MQ=255)

                   tCCCCCATAATGGCGTTGAGATCCGGCGTGGAGATAATTAGTTTTTCACCAACGGAAATCGCTTTATCTAAAACAGTCCCTTCCTGATTAGCGACAATTTGCGCGCCGGGAACGCGGGATTTTAAAACCTCTTCAAATCCTTTACGTCGc                                                                                                                          <  1:845221/150‑1 (MQ=255)

                   tCCCCCATAATGGCGTTGAGATCCGGCGTGGAGATAATTAGTTTTTCACCAACGGAAATCGCTTTATCTAAAACAGTCCCTTCCTGATTAGCGACAATTTGCGCGCCGGGAACGCGGGATTTTAAAACCTCTTCAAATCCTTTACGTCGc                                                                                                                          <  1:336931/150‑1 (MQ=255)

                   tCCCCCATAATGGCGTTGAGATCCGGCGTGGAGATAATTAGTTTTTCACCAACGGAAATCGCTTTATCTAAAACAGTCCCTTCCTGATTAGCGACAATTTGCGCGCCGGGAACGCGGGATTTTAAAACCTCTTCAAATCCTTTACGTCGCt                                                                                                                         >  1:81113/1‑151 (MQ=255)

                    cccccATAATGGCGTTGAGATCCGGCGTGGAGATAATTAGTTTTTCACCAACGGAAATCGCTTTATCTAAAACAGTCCCTTCCTGATTAGCGACAATTTGCGCGCCGGGAACGCGGGATTTTAAAACCTCTTCAAATCCTTTACGTCGCTg                                                                                                                        >  2:699151/1‑151 (MQ=255)

                    cccccATAATGGCGTTGAGATCCGGCGTGGAGATAATTAGTTTTTCACCAACGGAAATCGCTTTATCTAAAACAGTCCCTTCCTGATTAGCGACAATTTGCGCGCCGGGAACGCGGGATTTTAAAACCTCTTCAAATCCTTTACGTCGCTg                                                                                                                        >  2:490245/1‑151 (MQ=255)

                        cATAATGGCGTTGAGATCCGGCGTGGAGATAATTAGTTTTTCACCAACGGAAATCGCTTTATCTAAAACAGTCCCTTCCTGATTAGCGACAATTTGCGCGCCGGGAACGCGGGATTTTAAAACCTCTTCAAATCCTTTACGTCGCTGcac                                                                                                                     >  2:755070/1‑150 (MQ=255)

                          tAATGGCGTTGAGATCCGGCGTGGAGATAATTAGTTTTTCACCAACGGAAATCGCTTTATCTAAAACAGTCCCTTCCTGATTAGCGACAATTTGCGCGCCGGGAACGCGGGATTTTAAAACCTCTTCAAATCCTTTACGTCGCTGcacac                                                                                                                   >  1:309643/1‑150 (MQ=255)

                                 gTTGAGATCCGGCGTGGAGATAATTAGTTTTTCACCAACGGAAATCGCTTTATCTAAAACAGTCCCTTCCTGATTAGCGACAATTTGCGCGCCGGGAACGCGGGATTTTAAAACCTCTTCAAATCCTTTACGTCGCTGCACACAAACTTCa                                                                                                           <  2:275810/151‑1 (MQ=255)

                                         ccGGCGTGGAGATAATTAGTTTTTCACCAACGGAAATCGCTTTATCTAAAACAGTCCCTTCCTGATTAGCGACAATTTGCGCGCCGGGAACGCGGGATTTTAAAACCTCTTCAAATCCTTTACGTCGCTGCACACAAACTTCAAAGGCTTc                                                                                                   <  2:553950/151‑1 (MQ=255)

                                                ggAGATAATTAGTTTTTCACCAACGGAAATCGCTTTATCTAAAACAGTCCCTTCCTGATTAGCGACAATTTGCGCGCCGGGAACGCGGGATTTTAAAACCTCTTCAAATCCTTTACGTCGCTGCACACAAACTTCAAAGGCTTCGCAAtt                                                                                             <  2:807900/150‑1 (MQ=255)

                                                ggAGATAATTAGTTTTTCACCAACGGAAATCGCTTTATCTAAAACAGTCCCTTCCTGATTAGCGACAATTTGCGCGCCGGGAACGCGGGATTTTAAAACCTCTTCAAATCCTTTACGTCGCTGCACACAAACTTCAAAGGCTTCGCAATtg                                                                                            <  1:577333/151‑1 (MQ=255)

                                                   gaTAATTAGTTTTTCACCAACGGAAATCGCTTTATCTAAAACAGTCCCTTCCTGATTAGCGACAATTTGCGCGCCGGGAACGCGGGATTTTAAAACCTCTTCAAATCCTTTACGTCGCTGCACACAAACTTCAAAGGCTTCGCAATtgatg                                                                                         <  2:890136/151‑1 (MQ=255)

                                                       aTTAGTTTTTCACCAACGGAAATCGCTTTATCTAAAACAGTCCCTTCCTGATTAGCGACAATTTGCGCGCCGGGAACGCGGGATTTTAAAACCTCTTCAAATCCTTTACGTCGCTGCACACAAACTTCAAAGGCTTCGCAATTGATGACgg                                                                                     <  1:706502/151‑1 (MQ=255)

                                                          aGTTTTTCACCAACGGAAATCGCTTTATCTAAAACAGTCCCTTCCTGATTAGCGACAATTTGCGCGCCGGGAACGCGGGATTTTAAAACCTCTTCAAATCCTTTACGTCGCTGCACACAAACTTCAAAGGCTTCGCAATTGATGACGGCa                                                                                   >  1:870247/1‑150 (MQ=255)

                                                          aGTTTTTCACCAACGGAAATCGCTTTATCTAAAACAGTCCCTACCTGATTAGCGACAATTTGCGCGCCGGGAACGCGGGATTTTAAAACCTCTTCAAATCCTTTACGTCGCTGCACACAAACTTCAAAGGCTTCGCAATTGATGACGGCa                                                                                   <  1:260002/150‑1 (MQ=255)

                                                           gTTTTTCACCAACGGAAATCGCTTTATCTAAAACAGTCCCTTCCTGATTAGCGACAATTTGCGCGCCGGGAACGCGGGATTTTAAAACCTCTTCAAATCCTTTACGTCGCTGCACACAAACTTCAAAGGCTTCGCAATTGATGACGGCAAt                                                                                 >  1:321424/1‑151 (MQ=255)

                                                           gTTTTTCACCAACGGAAATCGCTTTATCTAAAACAGTCCCTTCCTGATTAGCGACAATTTGCGCGCCGGGAACGCGGGATTTTAAAACCTCTTCAAATCCTTTACGTCGCTGCACACAAACTTCAAAGGCTTCGCAATTGATGACGGCAAt                                                                                 >  2:241218/1‑151 (MQ=255)

                                                              tttCACCAACGGAAATCGCTTTATCTAAAACAGTCCCTTCCTGATTAGCGACAATTTGCGCGCCGGGAACGCGGGATTTTAAAACCTCTTCAAATCCTTTACGTCGCTGCACACAAACTTCAAAGGCTTCGCAATTGATGACGGCAAttt                                                                               <  1:148945/150‑1 (MQ=255)

                                                              tttCACCAACGGAAATCGCTTTATCTAAAACAGTCCCTTCCTGATTAGCGACAATTTGCGCGCCGGGAACGCGGGATTTTAAAACCTCTTCAAATCCTTTACGTCGCTGCACACAAACTTCAAAGGCTTCGCAATTGATGACGGCAAttt                                                                               <  1:36194/150‑1 (MQ=255)

                                                                tCACCAACGGAAATCGCTTTATCTAAAACAGTCCCTTCCTGATTAGCGACAATTTGCGCGCCGGGAACGCGGGATTTTAAAACCTCTTCAAATCCTTTACGTCGCTGCACACAGACTTCAAAGGCTTCGCAATTGATGACGGCAATTTTCg                                                                            >  1:493065/1‑151 (MQ=255)

                                                                tCACCAACGGAAATCGCTTTATCTAAAACAGTCCCTTCCTGATTAGCGACAATTTGCGCGCCGGGAACGCGGGATTTTAAAACCTCTTCAAATCCTTTACGTCGCTGCACACAAACTTCAAAGGCTTCGCAATTGATGACGGCAATTTTc                                                                             <  2:117682/150‑1 (MQ=255)

                                                                tCACCAACGGAAATCGCTTTATCTAAAACAGTCCCTTCCTGATTAGCGACAATTTGCGCGCCGGGAACGCGGGATTTTAAAACCTCTTCAAATCCTTTACGTCGCTGCACACAAACTTCAAAGGCTTCGCAATTGATGACGGCAATTTTc                                                                             <  1:103656/150‑1 (MQ=255)

                                                                   ccAACGGAAATCGCTTTATCTAAAACAGTCCCTTCCTGATTAGCGACAATTTGCGCGCCGGGAACGCGGGATTTTAAAACCTCTTCAAATCCTTTACGTCGCTGCACACAAACTTCAAAGGCTTCGCAATTGATGACGGCAATTTTCGGCt                                                                         >  1:386061/1‑151 (MQ=255)

                                                                           aaTCGCTTTATCTAAAACAGTCCCTTCCTGATTAGCGACAATTTGCGCGCCGGGAACGCGGGATTTTAAAACCTCTTCAAATCCTTTACGTCGCTGCACACAAACTTCAAAGGCTTCGCAATTGATGACGGCAATTTTCGGCTGGTCAAtt                                                                 >  2:530933/1‑151 (MQ=255)

                                                                             tCGCTTTATCTAAAACAGTCCCTTCCTGATTAGCGACAATTTGCGCGCCGGGAACGCGGGATTTTAAAACCTCTTCAAATCCTTTACGTCGCTGCACACAAACTTCAAAGGCTTCGCAATTGATGACGGCAATTTTCGGCTGGTCAAtttt                                                               >  2:793898/1‑151 (MQ=255)

                                                                               gCTTTATCTAAAACAGTCCCTTCCTGATTAGCGACAATTTGCGCGCCGGGAACGCGGGATTTTAAAACCTCTTCAAATCCTTTACGTCGCTGCACACAAACTTCAAAGGCTTCGCAATTGATGACGGCAATTTTCGGCTGGTCAATTTTAt                                                             >  1:537054/1‑151 (MQ=255)

                                                                                   tATCTAAAACAGTCCCTTCCTGATTAGCGACAATTTGCGCGCCGGGAACGCGGGATTTTAAAACCTCTTCAAATCCTTTACGTCGCTGCACACAAACTTCAAAGGCTTCGCAATTGATGACGGCAATTTTCGGCTGGTCAATTTTATTGGc                                                         >  2:111921/1‑151 (MQ=255)

                                                                                     tCTAAAACAGTCCCTTCCTGATTAGCGACAATTTGCGCGCCGGGAACGCGGGATTTTAAAACCTCTTCAAATCCTTTACGTCGCTGCACACAAACTTCAAAGGCTTCGCAATTGATGACGGCAATTTTCGGCTGGTCAATTTTATTGGCa                                                        >  2:176939/1‑150 (MQ=255)

                                                                                        aaaaCAGTCCCTTCCTGATTAGCGACAATTTGCGCGCCGGGAACGCGGGATTTTAAAACCTCTTCAAATCCTTTACGTCGCTGCACACAAACTTCAAAGGCTTCGCAATTGATGACGGCAATTTTCGGCTGGTCAATTTTATTGGCaataa                                                    <  1:165896/151‑1 (MQ=255)

                                                                                             aGTCCCTTCCTGATTAGCGACAATTTGCGCGCCGGGAACGCGGGATTTTAAAACCTCTTCAAATCCTTTACGTCGCTGCACACAAACTTCAAAGGCTTCGCAATTGATGACGGCAATTTTCGGCTGGTCAATTTTATTGGCaataaaataa                                               <  2:712157/151‑1 (MQ=255)

                                                                                              gTCCCTTCCTGATTAGCGACAATTTGCGCGCCGGGAACGCGGGATTTTAAAACCTCTTCAAATCCTTTACGTCGCTGCACACAAACTTCAAAGGCTTCGCAATTGATGACGGCAATTTTCGGCTGGTCAATTTTATTGGCAATAAaataat                                              >  2:104447/1‑151 (MQ=255)

                                                                                                cccTTCCTGATTAGCGACAATTTGCGCGCCGGGAACGCGGGATTTTGAAACCTCTTCAAATCCTTTACGTCGCTGCACACAAACTTCAAAGGCTTCGCAATTGATGACGGCAATTTTCGGCTGGTCAATTTTATTGGCAATAAAATAATc                                             <  1:763849/150‑1 (MQ=255)

                                                                                                   ttCCTGATTAGCGACAATTTGCGCGCCGGGAACGCGGGATTTTAAAACCTCTTCAAATCCTTTACGTCGCTGCACACAAACTTCAAAGGCTTCGCAATTGATGACGGCAATTTTCGGCTGGTCAATTTTATTGGCAATAAAATAATCAGCg                                         >  2:781369/1‑151 (MQ=255)

                                                                                                   ttCCTGATTAGCGACAATTTGCGCGCCGGGAACGCGGGATTTTAAAACCTCTTCAAATCCTTTACGTCGCTGCACACAAACTTCAAAGGCTTCGCAATTGATGACGGCAATTTTCGACTGGTCAATTTTATTGGCAATAAAATAATCAGCg                                         >  2:210708/1‑151 (MQ=255)

                                                                                                        gATTAGCGACAATTTGCGCGCCGGGAACGCGGGATTTTAAAACCTCTTCAAATCCTTTACGTCGCTGCACACAAACTTCAAAGGCTTCGCAATTGATGACGGCAATTTTCGGCTGGTCAATTTTATTGGCAATAAAATAATCAGCGGCa                                      <  2:153468/149‑1 (MQ=255)

                                                                                                        gATTAGCGACAATTTGCGCGCCGGGAACGCGGGATTTTAAAACCTCTTCAAATCCTTTACGTCGCTGCACACAAACTTCAAAGGCTTCGCAATTGATGACGGCAATTTTCGGCTGGTCAATTTTATTGGCAATAAAATAATCAGCGGCAGc                                    >  2:211615/1‑151 (MQ=255)

                                                                                                        gATTAGCGACAATTTGCGCGCCGGGAACGCGGGATTTTAAAACCTCTTCAAATCCTTTACGTCGCTGCACACAAACTTCAAAGGCTTCGCAATTGATGACGGCAATTTTCGGCTGGTCAATTTTATTGGCAATAAAATAATCAGCGGCAGc                                    >  2:824158/1‑151 (MQ=255)

                                                                                                            aGCGACAATTTGCGCGCCGGGAACGCGGGATTTTAAAACCTCTTCAAATCCTTTACGTCGCTGCACACAAACTTCAAAGGCTTCGCAATTGATGACGGCAATTTTCGGCTGGTCAATTTTATTGGCAATAAAATAATCAGCGGCAGCGTTa                                <  2:165670/151‑1 (MQ=255)

                                                                                                            aGCGACAATTTGCGCGCCGGGAACGCGGGATTTTAAAACCTCTTCAAATCCTTTACGTCGCTGCACACAAACTTCAAAGGCTTCGCAATTGATGACGGCAATTTTCGGCTGGTCAATTTTATTGGCAATAAAATAATCAGCGGCAGCGTTa                                <  2:17626/151‑1 (MQ=255)

                                                                                                              cGACAATTTGCGCGCCGGGAACGCGGGATTTTAAAACCTCTTCAAATCCTTTACGTCGCTGCACACAAACTTCAAAGGCTTCGCAATTGATGACGGCAATTTTCGGCTGGTCAATTTTATTGGCAATAAAATAATCAGCGGCAGCGTTAcc                              <  2:489685/151‑1 (MQ=255)

                                                                                                                       gcgcgcCGGGAACGCGGGATTTTAAAACCTCTTCAAATCCTTTACGTCGCTGCACACAAACTTCAAAGGCTTCGCAATTGATGACGGCAATTTTCGGCTGGTCAATTTTATTGGCAATAAAATAATCAGCGGCAGCGTTACCCAGtttttt                     <  2:265896/151‑1 (MQ=255)

                                                                                                                       gcgcgcCGGGAACGCGGGATTTTAAAACCTCTTCAAATCCTTTACGTCGCTGCACACAAACTTCAAAGGCTTCGCAATTGATGACGGCAATTTTCGGCTGGTCAATTTTATTGGCAATAAAATAATCAGCGGCAGCGTTACCCAGttttt                      <  1:96687/150‑1 (MQ=255)

                                                                                                                        cgcgcCGGGAACGCGGGATTTTAAAACCTCTTCAAATCCTTTACGTCGCTGCACACAAACTTCAAAGGCTTCGCAATTGATGACGGCAATTTTCGGCTGGTCAATTTTATTGGCAATAAAATAATCAGCGGCAGCGTTACCCAGTTTTTTa                    <  1:460531/151‑1 (MQ=255)

                                                                                                                           gcCGGGAACGCGGGATTTTAAAACCTCTTCAAATCCTTTACGTCGCTGCACACAAACTTCAAAGGCTTCGCAATTGATGACGGCAATTTTCGGCTGGTCAATTTTATTGGCAATAAAATAATCAGCGGCAGCGTTACCCAGTTTTTTAcc                  >  2:321202/1‑150 (MQ=255)

                                                                                                                              gggAACGCGGGATTTTAAAACCTCTTCAAATCCTTTACGTCGCTGCACACAAACTTCAAAGGCTTCGCAATTGATGACGGCAATTTTCGGCTGGTCAATTTTATTGGCAATAAAATAATCAGCGGCAGCGTTACCCAGTTTTTTACCAAAt              <  1:207222/151‑1 (MQ=255)

                                                                                                                               ggAACGCGGGATTTTAAAACCTCTTCAAATCCTTTACGTCGCTGCACACAAACTTCAAAGGCTTCGCAATTGATGACGGCAATTTTCGGCTGGTCAATTTTATTGGCAATAAAATAATCAGCGGCAGCGTTACCCAGTTTTTTACCAAAtt             <  1:542209/151‑1 (MQ=255)

                                                                                                                                    gcgGGATTTTAAAACCTCTTCAAATCCTTTACGTCGCTGCACACAAACTTCAAAGGCTTCGCAATTGATGACGGCAATTTTCGGCTGGTCAATTTTATTGGCAATAAAATAATCAGCGGCAGCGTTACCCAGTTTTTTACCAAATTCCAGc        <  2:472778/151‑1 (MQ=255)

                                                                                                                                     cgGGATTTTAAAACCTCTTCAAATCCTTTACGTCGCTGCACACAAACTTCAAAGGCTTCGCAATTGATGACGGCAATTTTCGGCTGGTCAATTTTATTGGCAATAAAATAATCAGCGGCAGCGTTACCCAGTTTTTTACCAAATTCCAgag       <  2:895031/151‑3 (MQ=255)

                                                                                                                                         aTTTTAAAGCCTCTTCAAATCCTTTACGTCGCTGCACACAAACTTCAAAGGCTTCGCAATTGATGACGGCAATTTTCGGCTGGTCAATTTTATTGGCAATAAAATAATCAGCGGCAGCGTTACCCAGTTTTTTACCAAATTCCAGCGGATc   <  1:629104/151‑1 (MQ=255)

                                                                                                                                           tttAAAACCTCTTCAAATCCTTTACGTCGCTGCACACAAACTTCAAAGGCTTCGCAATTGATGACGGCAATTTTCGGCTGGTCAATTTTATTGGCAATAAAATAATCAGCGGCAGCGTTACCCAGTTTTTTACCAAATTCCAGCGGATCg  <  2:287910/150‑1 (MQ=255)

GAGTGTCGCACCGCCCGATTCCCCCATAATGGCGTTGAGATCCGGCGTGGAGATAATTAGTTTTTCACCAACGGAAATCGCTTTATCTAAAACAGTCCCTTCCTGATTAGCGACAATTTGCGCGCCGGGAACGCGGGATTTTAAAACTTCTTCAAATCCTTTACGTCGCTGCACACAAACTTCAAAGGCTTCGCAATTGATGACGGCAATTTTCGGCTGGTCAATTTTATTGGCAATAAAATAATCAGCGGCAGCGTTACCCAGTTTTTTACCAAATTCCAGCGGATCG  >  NC_000913/2678669‑2678957

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A