Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A3 F50 I3 R1	283	58.3	1819865	98.8%	1798026	150.1

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,714,456	T→C	S289G (AGT→GGT)	yfiE ←	putative DNA‑binding transcriptional regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,714,456	0	T	C	100.0%	203.7 / NA	57	S289G (AGT→GGT)	yfiE	putative DNA‑binding transcriptional regulator
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (33/24); total (33/24)

CGTGGCTCATGCAATATATTGAATTTGCACGATCTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAATAAAGCGCACTTTGTCGACAAGTTAAATGCCGGGTTTAATCCCGACATTATTTTTATCCAGCCACAAAACTCTCTTCAACACACTGAATAAAAGTGTGCATCGCCGGGCTAACCGCTTTTCCAGCATGATGTGCGCACATGGCCGTAATGGTCTGCGACTGTTCGCCAAAAGGCAATTCAATTAATTCTCCGCACTCCAACTCTTTTGCCACCGCA  >  NC_000913/2714311‑2714601

cGTGGCTCATGCAATATATTGAATTTGCACGATCTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAATAAAGCGCACTTTGTCGACAAGTTAAATGCCGGGTTTAATCCCGACATTATTTTTATCCAGCCACAAAACCCTCtt                                                                                                                                              >  1:715306/1‑151 (MQ=255)

  tGGCTCATGCAATATATTGAATTTGCACGATCTTGTAGGGGGGATAAGGGGTGCAGGCCGCATCCGGCATGAATAAAGCGCACTTTGTCGGCAAGTTAAATGCCGGGTTTAAGCCCGACATTATTTTTATCCAGCCACAAAACCCTCtt                                                                                                                                              <  2:166881/149‑1 (MQ=37)

     cTCATGCAATATATTGAATTTGCACGATCTTGTAGGCCGGATAAGGCGCTCACGCCGCATCCGGCATGAATAAAGCGCACTTTGTCGACAAGTTAAATGCCGGGTTTAATCCCGACATTATTTTTATCCAGCCACAAAACCCTCTTCAac                                                                                                                                          >  2:748533/1‑150 (MQ=255)

              tatatTGAATTTGCACGATCTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAATAAAGCGCACTTTGTCGACAAGTTAAATGCCGGGTTTAATCCCGACATTATTTTTATCCAGCCACAAAACCCTCTTCAACACACTGAAt                                                                                                                                 >  2:268671/1‑150 (MQ=255)

               atatTGAATTTGCACGATCTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAATAAAGCGCACTTTGTCGACAAGTTAAATGCCGGGTTTAATCCCGACATTATTTTTATCCAGCCACAAAACCCTCTTCAACACACTGAATa                                                                                                                                >  2:630140/1‑150 (MQ=255)

                 atTGAATTTGCACGATCTTGAAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAATAAAGCGCACTTTGTCGACAAGTTAAATGCCGGGTTTAATCCCGACATTATTTTTATCCAGCCACAAAACCCTCTTCAACACACTGAATaaaa                                                                                                                             <  2:765260/151‑1 (MQ=255)

                  ttGAATTTGCACGATCTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAATAAAGCGCACTTTGTCGACAAGTTAAATGCCGGGTTTAATCCCGACATTATTTTTATCCAGCCACAAAACCCTCTTCAACACACTGAATaaa                                                                                                                              >  1:126405/1‑149 (MQ=255)

                    gAATTTGCACGATCTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAATAAAGCGCACTTTGTCGACAAGTTAAATGCCGGGTTTAATCCCGACATTATTTTTATCCAGCCACAAAACCCTCTTCAACACACTGAATAAAAgt                                                                                                                           <  1:110750/150‑1 (MQ=255)

                     aaTTTGCACGATCTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAATAAAGCGCACTTTGTCGACAAGTTAAATGCCGGGTTTAATCCCGACATTATTTTTATCCAGCCACAAAACCCTCTTCAACACACTGAATAAAAgtgt                                                                                                                         >  2:270122/1‑151 (MQ=255)

                     aaTTTGCACGATCTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAATAAAGCGCACTTTGTCGACAAGTTAAATGCCGGGTTTAATCCCGACATTATTTTTATCCAGCCACAAAACCCTCTTCAACACACTGAATAAAAgtgt                                                                                                                         >  1:799241/1‑151 (MQ=255)

                      aTTTGCACGATCTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAATAAAGCGCACTTTGTCGACAAGTTAAATGCCGGGTTTAATCCCGACATTATTTTTATCCAGCCACAAAACCCTCTTCAACACACTGAATAAAAGtgtg                                                                                                                        <  2:348449/151‑1 (MQ=255)

                         tGCACGATCTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAATAAAGCGCACTTTGTCGACAAGTTAAATGCCGGGTTTAATCCCGACATTATTTTTATCCAGCCACAAAACCCTCTTCAACACACTGAATAAAAGTGTGCAt                                                                                                                     >  1:273359/1‑151 (MQ=255)

                          gCACGATCTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAATAAAGCGCACTTTGTCGACAAGTTAAATGCCGGGTTTAATCCCGACATTATTTTTATCCAGCCACAAAACCCTCTTCAACACACTGAATAAAAGTGTGCa                                                                                                                      <  1:434063/149‑1 (MQ=255)

                          gCACGATCTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAATAAAGCGCACTTTGTCGACAAGTTAAATGCCGGGTTTAATCCCGACATTATTTTTATCCAGCCACAAAACCCTCTTCAACACACTGAATAAAAGTGTGCa                                                                                                                      <  2:739511/149‑1 (MQ=255)

                           cACGATCTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAATAAAGCGCACTTTGTCGACAAGTTAAATGCCGGGTTTAATCCCGACATTATTTTTATCCAGCCACAAAACCCTCTTCAACACACTGAATAAAAGTGTGCATCg                                                                                                                   <  1:276565/151‑1 (MQ=255)

                              gATCTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAATAAAGCGCACTTTGTCGACAAGTTAAATGCCGGGTTTAATCCCGACATTATTTTTATCCAGCCACAAAACCCTCTTCAACACACTGAATAAAAGTGTGCATCGCCg                                                                                                                >  2:545178/1‑151 (MQ=255)

                               aTCTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAATAAAGCGCACTTTGTCGACAAGTTAAATGCCGGGTTTAATCCCGACATTATTTTTATCCAGCCACAAAACCCTCTTCAACACACTGAATAAAAGTGTGCATCGCCgg                                                                                                               >  2:586091/1‑151 (MQ=255)

                                         ccGGATAAGGCGTTCACGCCGCATCCGGCATGAATAAAGCGCACTTTGTCGACAAGTTAAATGCCGGGTTTAATCCCGACATTATTTTTATCCAGCCACAAAACCCTCTTCAACACACTGAATAAAAGTGTGCATCGCCGGGCTAACCGCt                                                                                                     <  2:303727/151‑1 (MQ=255)

                                                  gCGTTCACGCCGCATCCGGCATGAATAAAGCGCACTTTGTCGACAAGTTAAATGCCGGGTTTAATCCCGACATTATTTTTATCCAGCCACAAAACCCTCTTCAACACACTGAATAAAAGTGTGCATCGCCGGGCTAACCGCTTTTCCAGc                                                                                             >  2:297219/1‑150 (MQ=255)

                                                    gTTCACGCCGCATCCGGCATGAATAAAGCGCACTTTGTCGACAAGTTAAATGCCGGGTTTAATCCCGACATTATTTTTATCCAGCCACAAAACCCTCTTCAACACACTGAATAAAAGTGTGCATCGCCGGGCTAACCGCTTTTCCAGCatg                                                                                          >  1:139653/1‑151 (MQ=255)

                                                      tCACGCCGCATCCGGCATGAATAAAGCGCACTTTGTCGACAAGTTAAATGCCGGGTTTAATCCCGACATTATTTTTATCCAGCCACAAAACCCTCTTCAACACACTGAATAAAAGTGTGCATCGCCGGGCTAACCGCTTTTCCAGCatgat                                                                                        >  2:843582/1‑151 (MQ=255)

                                                       cACGCCGCATCCGGCATGAATAAAGCGCACTTTGTCGACAAGTTAAATGCCGGGTTTAATCCCGACATTATTTTTATCCAGCCACAAAACCCTCTTCAACACACTGAATAAAAGTGTGCATCGCCGGGCTAACCGCTTTTCCAGCatga                                                                                         <  1:56029/149‑1 (MQ=255)

                                                               aTCCGGCATGAATAAAGCGCACTTTGTCGACAAGTTAAATGCCGGGTTTAATCCCGACATTATTTTTATCCAGCCACAAAACCCTCTTCAACACACTGAATAAAAGTGTGCATCGCCGGGCTAACCGCTTTTCCAGCATGATGTGCGcac                                                                                >  1:219046/1‑150 (MQ=255)

                                                                   ggCATGAATAAAGCGCACTTTGTCGACAAGTTAAATGCCGGGTTTAATCCCGACATTATTTTTATCCAGCCACAAAACCCTCTTCAACACACTGAATAAAAGTGTGCATCGCCGGGCTAACCGCTTTTCCAGCATGATGTGCGCACATGGc                                                                           <  1:752085/151‑1 (MQ=255)

                                                                     cATGAATAAAGCGCACTTTGTCGACAAGTTAAATGCCGGGTTTAATCCCGACATTATTTTTATCCAGCCACAAAACCCTCTTCAACACACTGAATAAAAGTGTGCATCGCCGGGGTAACCGCTTTTCCAGCATGATGTGCGCACATGGCCg                                                                         >  1:726201/1‑151 (MQ=255)

                                                                         aaTAAAGCGCACTTTGTCGACAAGTTAAATGCCGGGTTTAATCCCGACATTATTTTTATCCAGCCACAAAACCCTCTTCAACACACTGAATAAAAGTGTGCATCGCCGGGCTAACCGCTTTTCCAGCATGATGTGCGCACATGGCCGTaa                                                                      <  1:907587/150‑1 (MQ=255)

                                                                         aaTAAAGCGCACTTTGTCGACAAGTTAAATGCCGGGTTTAATCCCGACATTATTTTTATCCAGCCACAAAACCCTCTTCAACACACTGAATAAAAGTGTGCATCGCCGGGCTAACCGCTTTTCCAGCATGATGTGCGCACATGGCCGTAAt                                                                     <  1:554032/151‑1 (MQ=255)

                                                                             aaGCGCACTTTGTCGACAAGTTAAATGCCGGGTTTAATCCCGACATTATTTTTATCCAGCCACAAAACCCTCTTCAACACACTGAATAAAAGTGTGCATCGCCGGGCTAACCGCTTTTCCAGCATGATGTGCGCACATGGCCGTAATGGTc                                                                 >  1:429410/1‑151 (MQ=255)

                                                                               gcgcACTTTGTCGACAAGTTAAATGCCGGGTTTAATCCCGACATTATTTTTATCCAGCCACAAAACCCTCTTCAACACACTGAATAAAAGTGTGCATCGCCGGGCTAACCGCTTTTCCAGCATGATGTGCGCACATGGCCGTAATGGTCTg                                                               >  2:777046/1‑151 (MQ=255)

                                                                                 gcACTTTGTCGACAAGTTAAATGCCGGGTTTAATCCCGACATTATTTTTATCCAGCCACAAAACCCTCTTCAACACACTGAATAAAAGTGTGCATCGCCGGGCTAACCGCTTTTCCAGCATGATGTGCGCACATGGCCGTAATGGTCTGCg                                                             <  1:317106/151‑1 (MQ=255)

                                                                                     tttGTCGACAAGTTAAATGCCGGGTTTAATCCCGACATTATTTTTATCCAGCCACAAAACCCTCTTCAACACACTGAATAAAAGTGTGCATCGCCGGGCTAACCGCTTTTCCAGCATGATGTGCGCACATGGCCGTAATGGTCTGCGACTg                                                         >  1:708298/1‑151 (MQ=255)

                                                                                     tttGTCGACAAGTTAAATGCCGGGTTTAATCCCGACATTATTTTTATCCAGCCACAAAACCCTCTTCAACACACTGAATAAAAGTGTGCATCGCCGGGCTAACCGCTTTTCCAGCATGATGTGCGCACATGGCCGTAATGGTCTGCGACTg                                                         >  2:195955/1‑151 (MQ=255)

                                                                                       tGTCGACAAGTTAAATGCCGGGTTTAATCCCGACATTATTTTTATCCAGCCACAAAACCCTCTTCAACACACTGAATAAAAGTGTGCATCGCCGGGCTAACCGCTTTTCCAGCATGATGTGCGCACATGGCCGTAATGGTCTGCGACTGtt                                                       >  1:501430/1‑151 (MQ=255)

                                                                                        gTCGACAAGTTAAATGCCGGGTTTAATCCCGACATTATTTTTATCCAGCCACAAAACCCTCTTTAACACACTGAATAAAAGTGTGCATCGCCGGGCTAACCGCTTTTCCAGCATGATGTGCGCACATGGCCGTAATGGTCTGCGACTGtt                                                       <  1:490548/150‑1 (MQ=255)

                                                                                           gACAAGTTAAATGCCGGGTTTAATCCCGACATTATTTTTATCCAGCCACAAAACCCTCTTCAACACACTGAATAAAAGTGTGCATCGCCGGGCTAACCGCTTTTCCAGCATGATGTGCGCACATGGCCGTAATGGTCTGCGACTGTTCGcc                                                   >  2:727587/1‑151 (MQ=255)

                                                                                             cAAGTTAAATGCCGGGTTTAATCCCGACATTATTTTTATCCAGCCACAAAACCCTCTTCAACACACTGAATAAAAGTGTGCATCGCCGGGCTAACCGCTTTTCCAGCATGATGTGCGCACATGGCCGTAATGGTCTGCGACTGTTCGCCa                                                  >  2:310344/1‑150 (MQ=255)

                                                                                                       gCCGGGTTTAATCCCGACATTATTTTTATCCAGCCACAAAACCCTCTTCAACACACTGAATAAAAGTGTGCATCGCCGGGCTAACCGCTTTTCCAGCATGATGTGCGCACATGGCCGTAATGGTCTGCGACTGTTCGCCAAAAGGcaattc                                       >  2:491883/1‑151 (MQ=255)

                                                                                                       gCCGGGTTTAATCCCGACATTATTTTTATCCAGCCACAAAACCCTCTTCAACACACTGAATAAAAGTGTGCATCGCCGGGCTAACCGCTTTTCCAGCATGATGTGCGCACATGGCCGTAATGGTCTGCGACTGTTCGCCAAAAGGcaattc                                       >  2:612027/1‑151 (MQ=255)

                                                                                                       gCCGGGTTTAATCCCGACATTATTTTTATCCAGCCACAAAACCCTCTTCAACACACTGAATAAAAGTGTGCATCGCCGGGCTAACCGCTTTTCCAGCATGATGTGCGCACATGGCCGTAATGGTCTGCGACTGTTCGCCAAAAGGcaatt                                        <  1:105594/150‑1 (MQ=255)

                                                                                                        ccGGGTTTAATCCCGACATTATTTTTATCCAGCCACAAAACCCTCTTCAACACACTGAATAAAAGTGTGCATCGCCGGGCTAACCGCTTTTCCAGCATGATGTGCGCACATGGCCGTAATGGTCTGCGACTGTTCGCCAAAAGGcaattca                                      <  1:837105/151‑1 (MQ=255)

                                                                                                              ttAATCCCGACATTATTTTTATCCAGCCACAAAACCCTCTTCAACACACTGAATAAAAGTGTGCATCGCCGGGCTAACCGCTTTTCCAGCATGATGTGCGCACATGGCCGTAATGGTCTGCGACTGTTCGCCAAAAGGCAATTCaattaat                                >  1:544924/1‑151 (MQ=255)

                                                                                                                aaTCCCGACATTATTTTTATCCAGCCACAAAACCCTCTTCAACACACTGAATAAAAGTGTGCATCGCCGGGCTAACCGCTTTTCCAGCATGATGTGCGCACATGGCCGTAATGGTCTGCGACTGTTCGCCAAAAGGCAATTCaattaatt                               <  1:624784/150‑1 (MQ=255)

                                                                                                                aaTCCCGACATTATTTTTATCCAGCCACAAAACCCTCTTCAACACACTGAATAAAAGTGTGCATCGCCGGGCTAACCGCTTTTCCAGCATGATGTGCGCACATGGCCGTAATGGTCTGCGACTGTTCGCCAAAAGGCAATTCaattaatt                               <  2:811948/150‑1 (MQ=255)

                                                                                                                aaTCCCGACATTATTTTTATCCAGCCACAAAACCCTCTTCAACACACTGAATAAAAGTGTGCATCGCCGGGCTAACCGCTTTTCCAGCATGATGTGCGCACATGGCCGTAATGGTCTGCGACTGTTCGCCAAAAGGCAATTCAATTAATtc                              <  1:257036/151‑1 (MQ=255)

                                                                                                                   cccGACATTATTTTTATCCAGCCACAAAACCCTCTTCAACACACTGAATAAAAGTGTGCATCGCCGGGCTAACCGCTTTTCCAGCATGATGTGCGCACATGGCCGTAATGGTCTGCGACTGTTCGCCAAAAGGCAATTCAATTAATTCTcc                           >  2:118359/1‑151 (MQ=255)

                                                                                                                    ccGACATTATTTTTATCCAGCCACAAAACCCTCTTCAACACACTGAATAAAAGTGTGCATCGCCGGGCTAACCGCTTTTCCAGCATGATGTGCGCACATGGCCGTAATGGTCTGCGACTGTTCGCCAAAAGGCAATTCAATTAATTCTCCg                          <  1:245666/151‑1 (MQ=255)

                                                                                                                      gACATTATTTTTATCCAGCCACAAAACCCTCTTCAACACACTGAATAAAAGTGTGCATCGCCGGGCTAACCGCTTTTCCAGCATGATGTGCGCACATGGCCGTAATGGTCTGCGACTGTTCGCCAAAAGGCAATTCAATTAATTCTCCGc                         >  1:416893/1‑150 (MQ=255)

                                                                                                                       aCATTATTTTTATCCAGCCACAAAACCCTCTTCAACACACTGAATAAAAGTGTGCATCGCCGGGCTAACCGCTTTTCCAGCATGATGTGCGCACATGGCCGTAATGGTCTGCGACTGTTCGCCAAAAGGCAATTCAATTAATTCTCCGCAc                       <  2:196586/151‑1 (MQ=255)

                                                                                                                          ttattTTTATCCAGCCACAAAACCCTCTTCAACACACTGAATAAAAGTGTGCATCGCCGGGCTAACCGCTTTTCCAGCATGATGTGCGCACATGGCCGTAATGGTCTGCGACTGTTCGCCAAAAGGCAATTCAATTAATTCTCCGCACTcc                    >  1:550189/1‑151 (MQ=255)

                                                                                                                           tattTTTATCCAGCCACAAAACCCTCTTCAACACACTGAATAAAAGTGTGCATCGCCGGGCTAACCGCTTTTCCAGCATGATGTGCGCACATGGCCGTAATGGTCTGCGACTGTTCGCCAAAAGGCAATTCAATTAATTCTCCGCACTcc                    >  2:63283/1‑150 (MQ=255)

                                                                                                                            attTTTATCCAGCCACAAAACCCTCTTCAACACACTGAATAAAAGTGTGCATCGCCGGGCTAACCGCTTTTCCAGCATGATGTGCGCACATGGCCGTAATGGTCTGCGACTGTTCGCCAAAAGGCAATTCAATTAATTCTCCGCACTCCa                   >  1:423644/1‑150 (MQ=255)

                                                                                                                               tttATCCAGCCACAAAACCCTCTTCAACACACTGAATAAAAGTGTGCATCGCCGGGCTAACCGCTTTTCCAGCATGATGTGCGCACATGGCCGTAATGGTCTGCGACTGTTCGCCAAAAGGCAATTCAATTAATTCTCCGCACTCCAActc               >  1:433607/1‑151 (MQ=255)

                                                                                                                                 tATCCAGCCACAAAACCCTCTTCAACACACTGAATAAAAGTGTGCATCGCCGGGCTAACCGCTTTTCCAGCATGATGTGCGCACATGGCCGTAATGGTCTGCGACTGTTCGCCAAAAGGCAATTCAATTAATTCTCCGCACTCCAActct              <  1:111923/150‑1 (MQ=255)

                                                                                                                                  agccAGCCACAAAACCCTCTTCAACCCACTGAATAAAAGTGTGCATCGCCGGGCTAACCGCTTTTCCAGCATGATGTGCGCACATGGCCGTAGTGGTCTGCGACTGTTCGCCAAAACGCAATTCAATTAAGTCTCCGCACTCCAAc                 <  2:61319/144‑1 (MQ=255)

                                                                                                                                           caAAACCCTCTTCAACACACTGAATAAAAGTGTGCATCGCCGGGCTAACCGCTTTTCCAGCATGATGTGCGCACATGGCCGTAATGGTCTGCGACTGTTCGCCAAAAGGCAATTCAATTAATTCTCCGCACTCCAACTCTTTTGCCACCGc   >  1:695964/1‑151 (MQ=255)

                                                                                                                                            aaaaCCCTCTTCAACACACTGAATAAAAGTGTGCATCGCCGGGCTAACCGCTTTTCCAGCATGATGTGCGCACATGGCCGTAATGGTCTGCGACTGTTCGCCAAAAGGCAATTCAATTAATTCTCCGCACTCCAACTCTTTTGCCACCGCa  <  1:630189/151‑1 (MQ=255)

                                                                                                                                             aaaCCCTCTTCAACACACTGAATAAAAGTGTGCATCGCCGGGCTAACCGCTTTTCCAGCATGATGTGCGCACATGGCCGTAATGGTCTGCGACTGTTCGCCAAAAGGCAATTCAATTAATTCTCCGCACTCCAACTCTTTTGCCACCGCa  >  1:706969/1‑150 (MQ=255)

CGTGGCTCATGCAATATATTGAATTTGCACGATCTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAATAAAGCGCACTTTGTCGACAAGTTAAATGCCGGGTTTAATCCCGACATTATTTTTATCCAGCCACAAAACTCTCTTCAACACACTGAATAAAAGTGTGCATCGCCGGGCTAACCGCTTTTCCAGCATGATGTGCGCACATGGCCGTAATGGTCTGCGACTGTTCGCCAAAAGGCAATTCAATTAATTCTCCGCACTCCAACTCTTTTGCCACCGCA  >  NC_000913/2714311‑2714601

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 17 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A