Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A3 F50 I3 R1	283	58.3	1819865	98.8%	1798026	150.1

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,813,385	A→G	N257S (AAC→AGC)	emrB →	multidrug efflux system protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,813,385	0	A	G	100.0%	148.9 / NA	44	N257S (AAC→AGC)	emrB	multidrug efflux system protein
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (18/26); total (18/26)

GGTTATTGGTATCGGCAGCCTGCAGATTATGCTCGACCGCGGTAAAGAGCTGGACTGGTTTTCATCACAGGAAATTATCATCCTTACCGTGGTGGCGGTGGTGGCTATCTGCTTCCTGATTGTCTGGGAGCTGACCGACGATAACCCGATAGTCGATCTGTCGTTGTTTAAGTCGCGCAACTTCACCATCGGCTGCTTGTGTATCAGCCTCGCGTATATGCTCTACTTCGGCGCTATTGTTCTGCTGCCGCAGTTGTTGCAGGAGGTCTACGGTTACACGGCGACCTGGG  >  NC_000913/2813242‑2813531

ggTTATTGGTATCGGCAGCCTGCAGATTATGCTCGACCGCGGTAAAGAGCTGGACTGGTTTTCATCACAGGAAATTATCATCCTTACCGTGGTGGCGGTGGTGGCTATCTGCTTCCTGATTGTCTGGGAGCTGACCGACGATAGCCCGAt                                                                                                                                              <  2:395736/150‑1 (MQ=255)

   tATTGGTATCGGCAGCCTGCAGATTATGCTCGACCGCGGTAAAGAGCTGGACTGGTTTTCATCACAGGAAATTATCATCCTTACCGTGGTGGCGGTGGTGGCTATCTGCTTCCTGATTGTCTGGGAGCTGACCGACGATAGCCCGATAGt                                                                                                                                           <  1:194184/150‑1 (MQ=255)

       ggTATCGGCAGCCTGCAGATTATGCTCGACCGCGGTAAAGAGCTGGACTGGTTTTCATCACAGGAAATTATCATCCTTACCGTGGTGGCGGTGGTGGCTATCTGCTTCCTGATTGTCTGGGAGCTGACCGACGATAGCCCGATAGTCGAt                                                                                                                                       <  1:76757/150‑1 (MQ=255)

          aTCGGCAGCCTGCAGATTATGCTCGACCGCGGTAAAGAGCTGGACTGGTTTTCATCACAGGAAATTATCATCCTTACCGTGGTGGCGGTGGTGGCTATCTGCTTCCTGATTGTCTGGGAGCTGACCGACGATAGCCCGATAGTCGATCTGt                                                                                                                                   >  1:12064/1‑151 (MQ=255)

               cAGCCTGCAGATTATGCTCGACCGCGGTAAAGAGCTGGACTGGTTTTCATCACAGGAAATTATCATCCTTACCGTGGTGGCGGTGGTGGCTATCTGCTTCCTGATTGTCTGGGAGCTGACCGACGATAGCCCGATAGTCGATCTGTCgttg                                                                                                                              <  1:59170/151‑1 (MQ=255)

                aGCCTGCAGATTATGCTCGACCGCGGTAAAGAGCTGGACTGGTTTTCATCACAGGAAATTATCATCCTTACCGTGGTGGCGGTGGTGGCTATCTGCTTCCTGATTGTCTGGGAGCTGACCGACGATAGCCCGATAGTCGATCTGTCgttgt                                                                                                                             >  1:257322/1‑151 (MQ=255)

                 gCCTGCAGATTATGCTCGACCGCGGTAAAGAGCTGGACTGGTTTTCATCACAGGAAATTATCATCCTTACCGTGGTGGCGGTGGTGGCTATCTGCTTCCTGATTGTCTGGGAGCTGACCGACGATAGCCCGATAGTCGATCTGTCgttgtt                                                                                                                            <  2:163495/151‑1 (MQ=255)

                    tGCAGATTATGCTCGACCGCGGTAAAGAGCTGGACTGGTTTTCATCACAGGAAATTATCATCCTTACCGTGGTGGCGGTGGTGGCTATCTGCTTCCTGATTGTCTGGGAGCTGACCGACGATAGCCCGATAGTCGATCTGTCGTTGTTTa                                                                                                                          >  1:581988/1‑150 (MQ=255)

                            aTGCTCGACCGCGGTAAAGAGCTGGACTGGTTTTCATCACAGGAAATTATCATCCTTACCGTGGTGGCGGTGGTGGCTATCTGCTTCCTGATTGTCTGGGAGCTGACCGACGATAGCCCGATAGTCGATCTGTCGTTGTTTAAGTCGCGCa                                                                                                                 <  1:484815/151‑1 (MQ=255)

                                 cGACCGCGGTAAAGAGCTGGACTGGTTTTCATCACAGGAAATTATCATCCTTACCGTGGTGGCGGTGGTGGCTATCTGCTTCCTGATTGTCTGGGAGCTGACCGACGATAGCCCGATAGTCGATCTGTCGTTGTTTAAGTCGCGCAACtt                                                                                                             <  1:422280/150‑1 (MQ=255)

                                 cGACCGCGGTAAAGAGCTGGACTGGTTTTCATCACAGGAAATTATCATCCTTACCGTGGTGGCGGTGGTGGCTATCTGCTTCCTGATTGTCTGGGAGCTGACCGACGATAGCCCGATAGTCGATCTGTCGTTGTTTAAGTCGCGCAACt                                                                                                              <  1:627418/149‑1 (MQ=255)

                                  gACCGCGGTAAAGAGCTGGACTGGTTTTCATCACAGGAAATTATCATCCTTACCGTGGTGGCGGTGGTGGCTATCTGCTTCCTGATTGTCTGGGAGCTGACCGACGATAGCCCGATAGTCGATCTGTCGTTGTTTAAGTCGCGCAACTTc                                                                                                            >  1:882102/1‑150 (MQ=255)

                                    ccGCGGTAAAGAGCTGGACTGGTTTTCATCACAGGAAATTATCATCCTTACCGTGGTGGCGGTGGTGGCTATCTGCTTCCTGATTGTCTGGGAGCTGACCGACGATAGCCCGATAGTCGATCTGTCGTTGTTTAAGTCGCGCAACTTCAcc                                                                                                         >  2:101996/1‑151 (MQ=255)

                                          tAAAGAGCTGGACTGGTTTTCATCACAGGAAATTATCATCCTTACCGTGGTGGCGGTGGTGGCTATCTGCTTCCTGATTGTCTGGGAGCTGACCGACGATAGCCCGATAGTCGATCTGTCGTTGTTTAAGTCGCGCAACTTCACCATCgg                                                                                                    >  2:785649/1‑150 (MQ=255)

                                                gCTGGACTGGTTTTCATCACAGGAAATTATCATCCTTACCGTGGTGGCGGTGGTGGCTATCTGCTTCCTGATTGTCTGGGAGCTGACCGACGATAGCCCGATAGTCGATCTGTCGTTGTTTAAGTCGCGCAACTTCACCATCGGCTGCtt                                                                                              <  2:756235/150‑1 (MQ=255)

                                                gCTGGACTGGTTTTCATCACAGGAAATTATCATCCTTACCGTGGTGGCGGTGGTGGCTATCTGCTTCCTGATTGTCTGGGAGCTGACCGACGATAGCCCGATAGTCGATCTGTCGTTGTTTAAGTCGCGCAACTTCACCATCGGCTGCTtg                                                                                             <  2:784105/151‑1 (MQ=255)

                                                          ttttCATCACAGGAAATTATCATCCTTACCGTGGTGGCGGTGGTGGCTATCTGCTTCCTGATTGTCTGGGAGCTGACCGACGATAGCCCGATAGTCGATCTGTCGTTGTTTAAGTCGCGCAACTTCACCATCGGCTGCTTGTGTATCAGcc                                                                                   >  1:302794/1‑151 (MQ=255)

                                                            ttCATCACAGGAAATTATCATCCTTACCGTGGTGGCGGTGGTGGCTATCTGCTTCCTGATTGTCTGGGAGCTGACCGACGATAGCCCGATAGTCGATCTGTCGTTGTTTAAGTCGCGCAACTTCACCATCGGCTGCTTGTGTATCAGCCt                                                                                  >  2:723539/1‑150 (MQ=255)

                                                               atcaCAGGAAATGATCATCCTTACCGTGGTGGCGGTGGTGGCTATCTGCTTCCTGATTGTCTGGGAGCTGACCGACGATAGCCCGATAGTCGATCTGTCGTTGTTTAAGTCGCGCAACTTCACCATCGGCTGCTTGTGTATCAGCCTcgcg                                                                              <  2:413917/151‑1 (MQ=255)

                                                                   caGGAAATTATCATCCTTACCGTGGTGGCGGTGGTGGCTATCTGCTTCCTGATTGTCTGGGAGCTGACCGACGATAGCCCGATAGTCGATCTGTCGTTGTTTAAGTCGCGCAACTTCACCATCGGCTGCTTGTATATCAGCCTCGCGtata                                                                          <  2:83/151‑1 (MQ=255)

                                                                     ggAAATTATCATCCTTACCGTGGTGGCGGTGGTGGCTATCTGCTTCCTGATTGTCTGGGAGCTGACCGACGATAGCCCGATAGTCGATCTGTCGTTGTTTAAGTCGCGCAACTTCACCATCGGCTGCTTGTGTATCAGCCTCGCGTATATg                                                                        >  1:324719/1‑151 (MQ=255)

                                                                      gAAATTATCATCCTTACCGTGGTGGCGGTGGTGGCTATCTGCTTCCTGATTGTCTGGGAGCTGACCGACGATAGCCCGATAGTCGATCTGTCGTTGTTTAAGTCGCGCAACTTCACCATCGGCTGCTTGTGTATCAGCCTCGCGTATATGc                                                                       <  1:473573/151‑1 (MQ=255)

                                                                           tATCATCCTTACCGTGGTGGCGGTGGTGGCTATCTGCTTCCTGATTGTCTGGGAGCTGACCGACGATAGCCCGATAGTCGATCTGTCGTTGTTTAAGTCGCGCAACTTCACCATCGGCTGCTTGTGTATCAGCCTCGCGTATATGCTCTAc                                                                  >  1:634877/1‑151 (MQ=255)

                                                                              catcCTTACCGTGGTGGCGGTGGTGGCTATCTGCTTCCTGATTGTCTGGGAGCTGACCGACGATAGCCCGATAGTCGATCTGTCGTTGTTTAAGTCGCGCAACTTCACCATCGGCTGCTTGTGTATCAGCCTCGCGTATATGCTCTACTTc                                                               >  1:27309/1‑151 (MQ=255)

                                                                              catcCTTACCGTGGTGGCGGTGGTGGCTATCTGCTTCCTGATTGTCTGGGAGCTGACCGACGATAGCCCGATAGTCGATCTGTCGTTGTTTAAGTCGCGCAACTTCACCATCGGCTGCTTGTGTATCAGCCTCGCGTATATGCTCTACTTc                                                               <  1:629493/151‑1 (MQ=255)

                                                                               atcCTTACCGTGGTGGCGGTGGTGGCTATCTGCTTCCTGATTGTCTGGGAGCTGACCGACGATAGCCCGATAGTCGATCTGTCGTTGTTTAAGTCGCGCAACTTCACCATCGGCTGCTTGTGTATCAGCCTCGCGTATATGCTCTACTTCg                                                              <  1:855816/151‑1 (MQ=255)

                                                                                    tACCGTGGTGGCGGTGGTGGCTATCTGCTTCCTGATTGTCTGGGAGCTGACCGACGATAGCCCGATAGTCGATCTGTCGTTGTTTAAGTCGCGCAACTTCACCATCGGCTGCTTGTGTATCAGCCTCGCGTATATGCTCTACTTCGGCGCt                                                         >  1:102931/1‑151 (MQ=255)

                                                                                          ggtggCGGTGGTGGCTATCTGCTTCCTGATTGTCTGGGAGCTGACCGACGATAGCCCGATAGTCGATCTGTCGTTGTTTAAGTCGCGCAACTTCACCATCGGCTGCTTGTGTATCAGCCTCGCGTATATGCTCTACTTCGGCGCTATTGt                                                    <  2:169806/150‑1 (MQ=255)

                                                                                              gCGGTGGTGGCTATCTGCTTCCTGATTGTCTGGGAGCTGACCGACGATAGCCCGATAGTCGATCTGTCGTTGTTTAAGTCGCGCAACTTCACCATCGGCTGCTTGTGTATCAGCCTCGCGTATATGCTCTACTTCGGCGCTATTGTTctgc                                               <  1:153263/151‑1 (MQ=255)

                                                                                              gCGGTGGTGGCTATCTGCTTCCTGATTGTCTGGGAGCTGACCGACGATAGCCCGATAGTCGATCTGTCGTTGTTTAAGTCGCGCAACTTCACCATCGGCTGCTTGTGTATCAGCCTCGCGTATATGCTCTACTTCGGCGCTATTGTTctgc                                               <  1:454657/151‑1 (MQ=255)

                                                                                                ggtggtGGCTATCTGCTTCCTGATTGTCTGGGAGCTGACCGACGATAGCCCGATAGTCGATCTGTCGTTGTTTAAGTCGCGCAACTTCACCATCGGCTGCTTGTGTATCAGCCTCGCGTATATGCTCTACTTCGGCGCTATTGTTctgctg                                             >  1:786572/1‑151 (MQ=255)

                                                                                                       gCTATCCGGCTCCTGATTGGCTGGGAACTGTCCGACGATAGATATATAGTCGATCTAACGTTGTTTAATTCGCGCAACTTCCCCATCGGCTGCTTGTGTAACAACCGCGCGTATATGCTCTACTTCGGCGTTTTTGTACTGCTGCCGCAgt                                      <  2:756132/151‑1 (MQ=255)

                                                                                                         tATCTGCTTCCTGATTGTCTGGGAGCTGACCGACGATAGCCCGATAGTCGATCTGTCGTTGTTTAAGTCGCGCAACTTCACCATCGGCTGCTTGTGTATCAGCCTCGCGTATATGCTCTACTTCGGCGCTATTGTTCTGCTGCCGCAgttg                                    >  1:828854/1‑151 (MQ=255)

                                                                                                              gCTTCCTGATTGTCTGGGAGCTGACCGACGATAGCCCGATAGTCGATCTGTCGTTGTTTAAGTCGCGCAACTTCACCATCGGCTGCTTGTGTATCAGCCTCGCGTATATGCTCTACTTCGGCGCTATTGTTCTGCTGCCGCAGttgttg                                 <  2:297776/149‑1 (MQ=255)

                                                                                                              gCTTCCTGATTGTCTGGGAGCTGACCGACGATAGCCCGATAGTCGATCTGTCGTTGTTTAAGTCGCGCAACTTCACCATCGGCTGCTTGTGTATCAGCCTCGCGTATATGCTCTACTTCGGCGCTATTGTTCTGCTGCCGCAGTTGTTGc                                >  1:85663/1‑150 (MQ=255)

                                                                                                              gCTTCCTGATTGTCTGGGAGCTGACCGACGATAGCCCGATAGTCGATCTGTCGTTGTTTAAGTCGCGCAACTTCACCATCGGCTGCTTGTGTATCAGCCTCGCGTATATGCTCTACTTCGGCGCTATTGTTCTGCTGCCGCAGTTGTTGCa                               <  2:884350/151‑1 (MQ=255)

                                                                                                                             gggAGCTGACCGACGATAGCCCGATAGTCGATCTGTCGTTGTTTAAGTCGCGCAACTTCACCATCGGCTGCTTGTGTATCAGCCTCGCGTATATGCTCTACTTCGGCGCTATTGTTCTGCTGCCGCAGTTGTTGCAGGAGGTCTACgg                   <  1:609065/148‑1 (MQ=255)

                                                                                                                             gggAGCTGACCGACGATAGCCCGATAGTCGATCTGTCGTTGTTTAAGTCGCGCAACTTCACCATCGGCTGCTTGTGTATCAGCCTCGCGTATATGCTCTACTTCGGCGCTATTGTTCTGCTGCCGCAGTTGTTGCAGGAGGTCTACGGtt                 <  2:302019/150‑1 (MQ=255)

                                                                                                                              ggAGCTGACCGACGATAGCCCGATAGTCGATCTGTCGTTGTTTAAGTCGCGCAACTTCACCATCGGCTGCTTGTGTATCAGCCTCGCGTATATGCTCTACTTCGGCGCTATTGTTCTGCTGCCGCAGTTGTTGCAGGAGGTCTACGGTTac               <  2:634828/151‑1 (MQ=255)

                                                                                                                               gAGCTGACCGACGATAGCCCGATAGTCGATCTGTCGTTGTTTAAGTCGCGCAACTTCACCATCGGCTGCTTGTGTATCAGCCTCGCGTATATGCTCTACTTCGGCGCTATTGTTCTGCTGCCGCAGTTGTTGCAGGAGGTCTACGGTTaca              <  1:490732/151‑1 (MQ=255)

                                                                                                                                 gCTGACCGACGATAGCCCGATAGTCGATCTGTCGTTGTTTAAGTCGCGCAACTTCACCATCGGCTGCTTGTGTATCAGCCTCGCGTATATGCTCTACTTCGGCGCTATTGTTCTGCTGCCGCAGTTGTTGCAGGAGGTCTACGGTTACACg            >  2:635020/1‑151 (MQ=255)

                                                                                                                                    gACCGACGATAGCCCGATAGTCGATCTGTCGTTGTTTAAGTCGCGCAACTTCACCATCGGCTGCTTGTGTATCAGCCTCGCGTATATGCTCTACTTCGGCGCTATTGTTCTGCTGCCGCAGTTGTTGCAGGAGGTCTACGGTTACACGGc          <  2:365709/150‑1 (MQ=255)

                                                                                                                                       cgacgaTAGCCCGATAGTCGATCTGTCGTTGTTTAAGTCGCGCAACTTCACCATCGGCTGCTTGTGTATCAGCCTCGCGTATATGCTCTACTTCGGCGCTATTGTTCTGCTGCCGCAGTTGTTGCAGGAGGTCTACGGTTACACGGCGAcc      >  1:513625/1‑151 (MQ=255)

                                                                                                                                           gaTAGCCCGATAGTCGATCTGTCGTTGTTTAAGTCGCGCAACTTCACCATCGGCTGCTTGTGTATCAGCCTCGCGTATATGCTCTACTTCGGCGCTATTGTTCTGCTGCCGCAGTTGTTGCAGGAGGTCTACGGTTACACGGCGACCTggg  >  2:906383/1‑151 (MQ=255)

GGTTATTGGTATCGGCAGCCTGCAGATTATGCTCGACCGCGGTAAAGAGCTGGACTGGTTTTCATCACAGGAAATTATCATCCTTACCGTGGTGGCGGTGGTGGCTATCTGCTTCCTGATTGTCTGGGAGCTGACCGACGATAACCCGATAGTCGATCTGTCGTTGTTTAAGTCGCGCAACTTCACCATCGGCTGCTTGTGTATCAGCCTCGCGTATATGCTCTACTTCGGCGCTATTGTTCTGCTGCCGCAGTTGTTGCAGGAGGTCTACGGTTACACGGCGACCTGGG  >  NC_000913/2813242‑2813531

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A