Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A3 F50 I3 R1	283	58.3	1819865	98.8%	1798026	150.1

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,244,516	T→C	T76A (ACC→GCC)	uxaC ←	uronate isomerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,244,516	0	T	C	100.0%	179.3 / NA	50	T76A (ACC→GCC)	uxaC	uronate isomerase
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (27/23); total (27/23)

GCAGTTGACGGAGAAAGCAATTTGCCAGTGATACCAAACGGACGGCGCAGTTCGAGGTGCGTCCAGTGGTATAACGGGTTGCCGATAGTGTGCGGAACAGTCGCCGCCCAGGCGTCAAATTTTTCACGGTCAGACGCATCACCGGTACACAGACGCTCGGCCACACCGTTGGTACGCATAGCGCGCCATTTGTAGTGATCGCCTTTCAGCCAGATGTCATACAGGTTTTTAAAACGATAGTCTTCCGCAATCTGCTGCGGCGGCAAATGGCAATGGTAATCGAAAATCGGCTG  >  NC_000913/3244371‑3244663

gCAGTTGACGGAGAAAGCAATTTGCCAGTGATACCAAACGGACGGCGCAGTTCGAGGTGCGTCCAGTGGTATAACGGGTTGCCGATAGTGTGCGGAACAGTCGCCGCCCAGGCGTCAAATTTTTCACGGTCAGACGCATCACCGGCacac                                                                                                                                                 >  1:802431/1‑150 (MQ=255)

   gTTGACGGAGAAAGCAATTTGCCAGTGATACCAAACGGACGGCGCAGTTCGAGGTGCGTCCAGTGGTATAACGGGTTGCCGATAGTGTGCGGAACAGTCGCCGCCCAGGCGTCAAATTTTTCACGGTCAGACGCATCACCGGCACACAGAc                                                                                                                                             <  2:229512/151‑1 (MQ=255)

      gACGGAGAAAGCAATTTGCCAGTGATACCAAACGGACGGCGCAGTTCGAGGTGCGTCCAGTGGTATAACGGGTTGCCGATAGTGTGCGGAACAGTCGCCGCCCAGGCGTCAAATTTTTCACGGTCAGACGCATCACCGGCACACAGACGc                                                                                                                                           >  2:713316/1‑150 (MQ=255)

         ggAGAAAGCAATTTGCCAGTGATACCAAACGGACGGCGCAGTTCGAGGTGCGTCCAGTGGTATAACGGGTTGCCGATAGTGTGCGGAACAGTCGCCGCCCAGGCGTCAAATTTTTCACGGTCAGACGCATCACCGGCACACAGACGCTCgg                                                                                                                                       <  1:856308/151‑1 (MQ=255)

             aaaGCAATTTGCCAGTGATACCAAACGGACGGCGCAGTTCGAGGTGCGTCCAGTGGTATAACGGGTTGCCGATAGTGTGCGGAACAGTCGCCGCCCAGGCGTCAAATTTTTCACGGTCAGACGCATCACCGGCACACAGACGCTCGGCcac                                                                                                                                   >  1:310749/1‑151 (MQ=255)

                     ttGCCAGTGATACCAAACGGACGGCGCAGTTCGAGGTGCGTCCAGTGGTATAACGGGTTGCCGATAGTGTGCGGAACAGTCGCCGCCCAGGCGTCAAATTTTTCACGGTCAGACGCATCACCGGCACACAGACGCTCGGCCACACCGTTgg                                                                                                                           >  1:276016/1‑151 (MQ=255)

                     ttGCCAGTGATACCAAACGGACGGCGCAGTTCGAGGTGCGTCCAGTGGTATAACGGGTTGCCGATAGTGTGCGGAACAGACGCCGCCCAGGCGTCAAATTTTTCACGGTCAGACGCATCACCGGCACACAGACGCTCGGCCACACCGTTgg                                                                                                                           >  1:883184/1‑151 (MQ=255)

                        ccAGTGATACCAAACGGACGGCGCAGTTCGAGGTGCGTCCAGTGGTATAACGGGTTGCCGATAGTGTGCGGAACAGTCGCCGCCCAGGCGTCAAATTTTTCACGGTCAGACGCATCACCGGCACACAGACGCTCGGCCACACCGTTGGTAc                                                                                                                        >  2:113372/1‑151 (MQ=255)

                        ccAGTGATACCAAACGGACGGCGCAGTTCGAGGTGCGTCCAGTGGTATAACGGGTTGCCGATAGTGTGCGGAACAGTCGCCGCCCAGGCGTCAAATTTTTCACGGTCAGACGCATCACCGGCACACAGACGCCCGGCCACACCGTTGGTAc                                                                                                                        >  2:149452/1‑151 (MQ=255)

                           gTGATACCAAACGGACGGCGCAGTTCGAGGTGCGTCCAGTGGTATAACGGGTTGCCGATAGTGTGCGGAACAGTCGCCGCCCAGGCGTCAAATTTTTCACGGTCAGACGCATCACCGGCACACAGACGCTCGGCCACACCGTTGGTACGCa                                                                                                                     <  2:742470/151‑1 (MQ=255)

                                aCCAAACGGACGGCGCAGTTCGAGGTGCGTCCAGTGGTATAACGGGTTGCCGATAGTGTGCGGAACAGTCGCCGCCCAGGCGTCAAATTTTTCACGGTCAGACGCATCACCGGCACACAGACGCTCGGCCACACCGTTGGTACGCATAgc                                                                                                                 <  1:615815/150‑1 (MQ=255)

                                       ggacggCGCAGTTCGAGGTGCGTCCAGTGGTATAACGGGTTGCCGATAGTGTGCGGAACAGTCGCCGCCCAGGCGTCAAATTTTTCACGGTCAGACGCATCACCGGCACACAGACGCTCGGCCACACCGTTGGTACGCATAGCGCGCCAtt                                                                                                         <  1:854799/151‑1 (MQ=255)

                                         acggCGCAGTTCGAGGTGCGTCCAGTGGTATAACGGGTTGCCGATAGTGTGCGGAACAGTCGCCGCCCAGGCGTCAAATTTTTCACGGTCAGACGCATCACCGGCACACAGACGCTCGGCCACACCGTTGGTACGCATAGCGCGCCATTTg                                                                                                       <  1:577431/151‑1 (MQ=255)

                                                   tCGAGGTGCGTCCAGTGGTATAACGGGTTGCCGATAGTGTGCGGAACAGTCGCCGCCCAGGCGTCAAATTTTTCACGGTCAGACGCATCACCGGCACACAGACGCTCGGCCACACCGTTGGTACGCATAGCGCGCCATTTGTAGTGATCGc                                                                                             >  2:745543/1‑151 (MQ=255)

                                                    cGAGGTGCGTCCAGTGGTATAACGGGTTGCCGATAGTGTGCGGAACAGTCGCCGCCCAGGCGTCAAATTTTTCACGGTCAGACGCATCACCGGCACACAGACGCTCGGCCACACCGTTGGTACGCATAGCGCGCCATTTGTAGTGATCGcc                                                                                            >  1:151301/1‑151 (MQ=255)

                                                      aGGTGCGTCCAGTGGTATAACGGGTTGCCGATAGTGTGCGGAACAGTCGCCGCCCAGGCGTCAAATTTTTCACGGTCAGACGCATCACCGGCACACAGACGCTCGGCCACACCGTTGGTACGCATAGCGCGCCATTTGTAGTGATCGCCtt                                                                                          >  1:370922/1‑151 (MQ=255)

                                                          gCGTCCAGTGGTATAACGGGTTGCCGATAGTGTGCGGAACAGTCGCCGCCCAGGCGTCAAATTTTTCACGGTCAGACGCATCACCGGCACACAGACGCTCGGCCACACCGTTGGTACGCATAGCGCGCCATTTGTAGTGATCGCCTTTCa                                                                                       <  2:706312/150‑1 (MQ=255)

                                                            gTCCAGTGGTATAACGGGTTGCCGATAGTGTGCGGAACAGTCGCCGCCCAGGCGTCAAATTTTTCACGGTCAGACGCATCACCGGCACACAGACGCTCGGCCACACCGTTGGTACGCATAGCGCGCCATTTGTAGTGATCGCCTTTCAGcc                                                                                    <  1:177809/151‑1 (MQ=255)

                                                              ccAGTGGTATAACGGGTTGCCGATAGTGTGCGGAACAGTCGCCGCCCAGGCGTCAAATTTTTCACGGTCAGACGCATCACCGGCACACAGACGCTCGGCCACACCGTTGGTACGCATAGCGCGCCATTTGTAGTGATCGCCTTTCAGcc                                                                                    >  1:746838/1‑149 (MQ=255)

                                                              ccAGTGGTATAACGGGTTGCCGATAGTGTGCGGAACAGTCGCCGCCCAGGCGTCAAATTTTTCACGGTCAGACGCATCACCGGCACACAGACGCTCGGCCACACCGTTGGTACGCATAGCGCGCCATTTGTAGTGATCGCCTTTCAGCCa                                                                                   <  1:55986/150‑1 (MQ=255)

                                                              ccAGTGGTATAACGGGTTGCCGATAGTGTGCGGAACAGTCGCCGCCCAGGCGTCAAATTTTTCACGGTCAGACGCATCACCGGCACACAGACGCTCGGCCACACCGTTGGTACGCATAGCGCGCCATTTGTAGTGATCGCCTTTCAGCCAg                                                                                  <  2:66142/151‑1 (MQ=255)

                                                                     tataACGGGTTGCCGATAGTGTGCGGAACAGTCGCCGCCCAGGCGTCAAATTTTTCACGGTCAGACGCATCACCGGCACACAGACGCTCGGCCACACCGTTGGTACGCATAGCGCGCCATTTGTAGTGATCGCCTTTCAGCCAGATGTCAt                                                                           >  1:348909/1‑151 (MQ=255)

                                                                       taACGGGTTGCCGATAGTGTGCGGAACAGTCGCCGCCCAGGCGTCAAATTTTTCACGGTCAGACGCATCACCGGCACACAGACGCTCGGCCACACCGTTGGTACGCATAGCGCGCCATTTGTAGTGATCGCCTTTCAGCCAGATGTCATAc                                                                         >  2:61103/1‑151 (MQ=255)

                                                                        aaCGGGTTGCCGATAGTGTGCGGAACAGTCGCCGCCCAGGCGTCAAATTTTTCACGGTCAGACGCATCACCGGCACACAGACGCTCGGCCACACCGTTGGTACGCATAGCGCGCCATTTGTAGTGATCGCCTTTCAGCCAGATGTCATAc                                                                         >  2:861530/1‑150 (MQ=255)

                                                                           gggTTGCCGATAGTGTGCGGAACAGTCGCCGCCCAGGCGTCAAATTTTTCACGGTCAGACGCATCACCGGCACACAGACGCTCGGCCACACCGTTGGTACGCATAGCGCGCCATTTGTAGTGATCGCCTTTCAGCCAGATGTCATACAGGt                                                                     <  2:83404/151‑1 (MQ=255)

                                                                            ggTTGCCGATAGTGTGCGGAACAGTCGCCGCCCAGGCGTCAAATTTTTCACGGTCAGACGCATCACCGGCACACAGACGCTCGGCCACACCGTTGGTACGCATAGCGCGCCATTTGTAGTGATCGCCTTTCAGCCAGATGTCATACAGGtt                                                                    >  1:244622/1‑151 (MQ=255)

                                                                            ggTTGCCGATAGTGTGCGGAACAGTCGCCGCCCAGGCGTCAAATTTTTCACGGTCAGACGCATCACCGGCACACAGACGCTCGGCCACACCGTTGGTACGCATAGCGCGCCATTTGTAGTGATCGCCTTTCAGCCAGATGTCATACAGGtt                                                                    >  2:528645/1‑151 (MQ=255)

                                                                            ggTTGCCGATAGTGTGCGGAACAGTCGCCGCCCAGGCGTCAAATTTTTCACGGTCAGACGCATCACCGGCACACAGACGCTCGGCCACACCGTTGGTACGCATAGCGCGCCATTTGTAGTGATCGCCTTTCAGCCAGATGTCATACAGGtt                                                                    >  1:642136/1‑151 (MQ=255)

                                                                              ttGCCGATAGTGTGCGGAACAGTCGCCGCCCAGGCGTCAAATTTTTCACGGTCAGACGCATCACCGGCACACAGACGCTCGGCCACACCGTTGGTACGCATAGCGCGCCATTTGTAGTGATCGCCTTTCAGCCAGATGTCATACAGGtttt                                                                  >  1:160472/1‑151 (MQ=255)

                                                                               tGCCGATAGTGTGCGGAACAGTCGCCGCCCAGGCGTCAAATTTTTCACGGTCAGACGCATCACCGGCACACAGACGCTCGGCCACACCTTTGGTACGCATAGCGCGCCATTTGTAGTGATCGCCTTTCAGCCAGATGTCATACAGGtttt                                                                  <  2:588936/150‑1 (MQ=255)

                                                                                  cGATAGTGTGCGGAACAGTCGCCGCCCAGGCGTCAAATTTTTCACGGTCAGACGCATCACCGGCACACAGACGCTCGGCCACACCGTTGGTACGCATAGCGCGCCATTTGTAGTGATCGCCTTTCAGCCAGATGTCATACAGGTTTTTaaa                                                              <  1:581920/151‑1 (MQ=255)

                                                                                  cGATAGTGTGCGGAACAGTCGCCGCCCAGGCGTCAAATTTTTCACGGTCAGACGCATCACCGGCACACAGACGCTCGGCCACACCGTTGGTACGCATAGCGCGCCATTTGTAGTGATCGCCTTTCAGCCAGATGTCATACAGGTTTTTaaa                                                              <  1:231842/151‑1 (MQ=255)

                                                                                    aTAGTGTGCGGAACAGTCGCCGCCCAGGCGTCAAATTTTTCACGGTCAGACGCATCACCGGCACACAGACGCTCGGCCACACCGTTGGTACGCATAGCGCGCCATTTGTAGTGATCGCCTTTCAGCCAGATGTCATACAGGTTTTTAAAAc                                                            >  1:351946/1‑151 (MQ=255)

                                                                                     tAGTGTGCGGAACAGTCGCCGCCCAGGCGTCAAATTTTTCACGGTCAGACGCATCACCGGCACACAGACGCTCGGCCACACCGTTGGTACGCATAGCGCGCCATTTGTAGTGATCGCCTTTCAGCCAGATGTCATACAGGTTTTTAAAACg                                                           >  1:546253/1‑151 (MQ=255)

                                                                                      aGTGTGCGGAACAGTCGCCGCCCAGGCGTCAAATTTTTCACGGTCAGACGCATCACCGGCACACAGACGCTCGGCCACACCGTTGGTACGCATAGCGCGCCATTTGTAGTGATCGCCTTTCAGCCAGATGTCATACAGGTTTTTAAAACg                                                           <  2:392270/150‑1 (MQ=255)

                                                                                       gtgtGCGGAACAGTCGCCGCCCAGGCGTCAAATTTTTCACGGTCAGACGCATCACCGGCACACAGACGCTCGGCCACACCGTTGGTACGCATAGCGCGCCATTTGTAGTGATCGCCTTTCAGCCAGATGTCATACAGGTTTTTAAAACGAt                                                         <  1:571997/151‑1 (MQ=255)

                                                                                               aaCAGTCGCCGCCCAGGCGTCAAATTTTTCACGGTCAGACGCATCACCGGCACACAGACGCTCGGCCACACCGTTGGTACGCATAGCGCGCCATTTGTAGTGATCGCCTTTCAGCCAGATGTCATACAGGTTTTTAAAACGATAGTCTTcc                                                 >  1:703102/1‑151 (MQ=255)

                                                                                                 cAGTCGCCGCCCAGGCGTCAAATTTTTCACGGTCAGACGCATCACCGGCACACAGACGCTCGGCCACACCGTTGGTACGCATAGCGCGCCATTTGTAGTGATCGCCTTTCAGCCAGATGTCATACAGGTTTTTAAAACGATAGTCTTCCGc                                               <  2:684326/151‑1 (MQ=255)

                                                                                                    tCGCCGCCCAGGCGTCAAATTTTTCACGGTCAGACGCATCACCGGCACACAGACGCTCGGCCACACCGTTGGTACGCATAGCGCGCCATTTGTAGTGATCGCCTTTCAGCCAGATGTCATACAGGTTTTTAAAACGATAGTCTTCCGCAAt                                            >  2:400326/1‑151 (MQ=255)

                                                                                                                   cAAATTTTTCACGGTCAGACGCATCACCGGCACACAGACGCTCGGCCACACCGTTGGTACGCATAGCGCGCCATTTGTAGTGATCGCCTTTCAGCCAGATGTCATACAGGTTTTTAAAACGATAGTCTTCCGCAATCTGCTGCGGCGGCa                              >  2:424660/1‑150 (MQ=255)

                                                                                                                    aaaTTTTTCACGGTCAGACGCATCACCGGCACACAGACGCTCGGCCACACCGTTGGTACGCATAGCGCGCCATTTGTAGTGATCGCCTTTCAGCCAGATGTCATACAGGTTTTTAAAACGATAGTCTTCCGCAATCTGCTGCGGCGGCaa                             >  1:322969/1‑150 (MQ=255)

                                                                                                                      aTTTTTCACGGTCAGACGCATCACCGGCACACAGACGCTCGGCCACACCGTTGGTACGCATAGCGCGCCATTTGTAGTGATCGCCTTTCAGCCAGATGTCATACAGGTTTTTAAAACGATAGTCTTCCGCAATCTGCTGCGGCGGCAAATg                          >  2:776310/1‑151 (MQ=255)

                                                                                                                        ttttCACGGTCAGACGCATCACCGGCACACAGACGCTCGGCCACACCGTTGGTACGCATAGCGCGCCATTTGTAGTGATCGCCTTTCAGCCAGATGTCATACAGGTTTTTAAAACGATAGTCTTCCGCAATCTGCTGCGGCGGCAAATgg                         <  1:766668/150‑1 (MQ=255)

                                                                                                                             aCGGTCAGACGCATCACCGGCACACAGACGCTCGGCCACACCGTTGGTACGCATAGCGCGCCATTTGTAGTGATCGCCTTTCAGCCAGATGTCATACAGGTTTTTAAAACGATAGTCTTCCGCAATCTGCTGCGGCGGCAAATGGCAATgg                   <  2:263827/151‑1 (MQ=255)

                                                                                                                               ggTCAGACGCATCACCGGCACACAGACGCTCGGCCACACCGTTGGTACGCATAGCGCGCCATTTGTAGTGATCGCCTTTCAGCCAGATGTCATACAGGTTTTTAAAACGTTAGTCTTCCGCAATCTGCTGCGGCGGCAAATGGCAATGGt                  >  1:669134/1‑150 (MQ=255)

                                                                                                                               ggTCAGACGCATCACCGGCACACAGACGCTCGGCCACACCGTTGGTACGCATAGCGCGCCATTTGTAGTGATCGCCTTTCAGCCAGATGTCATACAGGTTTTTAAAACGATAGTCTTCCGCAATCTGCTGCGGCGGCAAATGGCAATGGTa                 <  2:746788/151‑1 (MQ=255)

                                                                                                                                gTCAGACGCATCACCGGCACACAGACGCTCGGCCACACCGTTGGTACGCATAGCGCGCCATTTGTAGTGATCGCCTTTCAGCCAGATGTCATACAGGTTTTTAAAACGATAGTCTTCCGCAATCTGCTGCGGCGGCAAATGGCAATGGTaa                <  1:440510/151‑1 (MQ=255)

                                                                                                                                        cATCACCGGCACACAGACGCTCGGCCACACCGTTGGTACGCATAGCGCGCCATTTGTAGTGATCGCCTTTCAGCCAGATGTCATACAGGTTTTTAAAACGATAGTCTTCCGCAATCTGCTGCGGCGGCAAATGGCAATGGTAATCGAAAAt        <  1:889510/151‑1 (MQ=255)

                                                                                                                                         aTCACCGGCACACAGACGCTCGGCCACACCGTTGGTACGCATAGCGCGCCATTTGTAGTGATCGCCTTTCAGCCAGATGTCATACAGGTTTTTAAAACGATAGTCTTCCGCAATCTGCTGCGGCGGCAAATGGCAATGGTAATCGAAAATc       <  2:322943/151‑1 (MQ=255)

                                                                                                                                              cGGCACACAGACGCTCGGCCACACCGTTGGTACGCATAGCGCGCCATTTGTAGTGATCGCCTTTCAGCCAGATGTCATACAGGTTTTTAAAACGATAGTCTTCCGCAATCTGCTGCGGCGGCAAATGGCAATGGTAATCGAAAATCGGCTg  >  1:705670/1‑151 (MQ=255)

GCAGTTGACGGAGAAAGCAATTTGCCAGTGATACCAAACGGACGGCGCAGTTCGAGGTGCGTCCAGTGGTATAACGGGTTGCCGATAGTGTGCGGAACAGTCGCCGCCCAGGCGTCAAATTTTTCACGGTCAGACGCATCACCGGTACACAGACGCTCGGCCACACCGTTGGTACGCATAGCGCGCCATTTGTAGTGATCGCCTTTCAGCCAGATGTCATACAGGTTTTTAAAACGATAGTCTTCCGCAATCTGCTGCGGCGGCAAATGGCAATGGTAATCGAAAATCGGCTG  >  NC_000913/3244371‑3244663

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A