Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A3 F50 I2 R1	257	49.9	1554294	99.0%	1538751	150.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,099,158	G→A	D94N (GAT→AAT)	ycdX →	alkaline phosphatase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,099,158	0	G	A	100.0%	140.4 / NA	37	D94N (GAT→AAT)	ycdX	alkaline phosphatase
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (23/14); total (23/14)

AAGATGCGCCGCATCACTGGCACTTCATTAACATGCGTATCTGGCCGCGAGTGGTTGATGGGGTAGGGATCCTGCGCGGCATCGAAGCTAACATTAAAAATGTTGATGGTGAAATTGACTGCAGCGGTAAAATGTTTGACTCGCTGGATCTAATTATTGCCGGTTTTCATGAGCCGGTTTTTGCGCCACATGACAAAGCGACCAATACACAAGCGATGATCGCCACTATCGCCAGCGGCAATGTGCATATAATAAGCCATCCCGGAAATCCCAAATATGAAATAGATGTGAAA  >  NC_000913/1099012‑1099304

aaGATGCGCCGCATCACTGGCACTTCATTAACATGCGTATCTGGCCGCGAGTGGTTGATGGGGTAGGGATCCTGCGCGGCATCGAAGCTAACATTAAAAATGTTGATGGTGAAATTGACTGCAGCGGTAAAATGTTTGACTCGCTGAATCt                                                                                                                                                <  2:360288/151‑1 (MQ=255)

 aGATGCGCCGCATCACTGGCACTTCATTAACATGCGTATCTGGCCGCGAGTGGTTGATGGGGTAGGGATCCTGCGCGGCATCGAAGCTAACATTAAAAATGTTGATGGTGAAATTGACTGCAGCGGTAAAATGTTTGACTCGCTGAATCt                                                                                                                                                >  1:46219/1‑150 (MQ=255)

       gccgcATCACTGGCACTTCATTAACATGCGTATCTGGCCGCGAGTGGTTGATGGGGTAGGGATCCTGCGCGGCATCGAAGCTAACATTAAAAATGTTGATGGTGAAATTGACTGCAGCGGTAAAATGTTTGACTCGCTGAATCTAattatt                                                                                                                                         >  2:749014/1‑151 (MQ=255)

            aTCACTGGCACTTCATTAACATGCGTATCTGGCCGCGAGTGGTTGATGGGGTAGGGATCCTGCGCGGCATCGAAGCTAACATTAAAAATGTTGATGGTGAAATTGACTGCAGCGGTAAAATGTTTGACTCGCTGAATCTAATTATTGCCgg                                                                                                                                    >  2:422716/1‑151 (MQ=255)

            aTCACTGGCACTTCATTAACATGCGTATCTGGCCGCGAGTGGTTGATGGGGTAGGGATCCTGCGCGGCATCGAAGCTAACATTAAAAATGTTGATGGTGAAATTGACTGCAGCGGTAAAATGTTTGACTCGCTGAATCTAATTATTGCCgg                                                                                                                                    >  1:20089/1‑151 (MQ=255)

                cTGGCACTTCATTAACATGCGTATCTGGCCGCGAGTGGTTGATGGGGTAGGGATCCTGCGCGGCATCGAAGCTAACATTAAAAATGTTGATGGTGAAATTGACTGCAGCGGTAAAATGTTTGACTCGCTGAATCTAATTATTGCCGGtttt                                                                                                                                >  2:723357/1‑151 (MQ=255)

                    cACTTCATTAACATGCGTATCTGGCCGCGAGTGGTTGATGGGGTAGGGATCCTGCGCGGCATCGAAGCTAACATTAAAAATGTTGATGGTGAAATTGACTGCAGCGGTAAAATGTTTGACTCGCTGAATCTAATTATTGCCGGTTTTCATg                                                                                                                            <  1:256471/151‑1 (MQ=255)

                             aaCATGCGTATCTGGCCGCGAGTGGTTGATGGGGTAGGGATCCTGCGCGGCATCGAAGCTAACATTAAAAATGTTGATGGTGAAATTGACTGCAGCGGTAAAATGTTTGACTCGCTGAATCTAATTATTGCCGGTTTTCATGAGCCGGttt                                                                                                                   >  1:665375/1‑151 (MQ=255)

                                            ccGCGAGTGGTTGATGGGGTAGGGATCCTGCGCGGCATCGAAGCTAACATTAAAAATGTTGATGGTGAAATTGACTGCAGCGGTAAAATGTTTGACTCGCTGAATCTAATTATTGCCGGTTTTCATGAGCCGGTTTTTGCGCCACATGACa                                                                                                    <  2:16127/151‑1 (MQ=255)

                                              gcgAGTGGTTGATGGGGTAGGGATCCTGCGCGGCATCGAAGCTAACATTAAAAATGTTGATGGTGAAATTGACTGCAGCGGTAAAATGTTTGACTCGCTGAATCTAATTATTGCCGGTTTTCATGAGCCGGTTTTTGCGCCACATGACaa                                                                                                   >  2:414585/1‑150 (MQ=255)

                                                    ggTTGATGGGGTAGGGATCCTGCGCGGCATCGAAGCTAACATTAAAAATGTTGATGGTGAAATTGACTGCAGCGGTAAAATGTTTGACTCGCTGAATCTAATTATTGCCGGTTTTCATGAGCCGGTTTTTGCGCCACATGACAAAGCGAc                                                                                             <  2:257938/150‑1 (MQ=255)

                                                            gggTAGGGATCCTGCGCGGCATCGAAGCTAACATTAAAAATGTTGATGGTGAAATTGACTGCAGCGGTAAAATGTTTGACTCGCTGAATCTAATTATTGCCGGTTTTCATGAGCCGGTTTTTGCGCCACATGACAAAGCGACCAATAcaca                                                                                    <  1:596611/151‑1 (MQ=255)

                                                              gTAGGGATCCTGCGCGGCATCGAAGCTAACATTAAAAATGTTGATGGTGAAATTGACTGCAGCGGTAAAATGTTTGACTCGCTGAATCTAATTATTGCCGGTTTTCATGAGCCGGTTTTTGCGCCACATGACAAAGCGACCAATAcaca                                                                                    >  2:397997/1‑149 (MQ=255)

                                                              gTAGGGATCCTGCGCGGCATCGAAGCTAACATTAAAAATGTTGATGGTGAAATTGACTGCAGCGGTAAAATGTTTGACTCGCTGAATCTAATTATTGCCGGTTTTCATGAGCCGGTTTTTGCGCCACATGACAAAGCGACCAATACACAAg                                                                                  <  1:719407/151‑1 (MQ=255)

                                                                          cgcgGCATCGAAGCTAACATTAAAAATGTTGATGGTGAAATTGACTGCAGCGGTAAAATGTTTGACTCGCTGAATCTAATTATTGCCGGTTTTCATGAGCCGGTTTTTGCGCCACATGACAAAGCGACCAATACACAAGCGATGATCGCCa                                                                      <  1:434423/151‑1 (MQ=255)

                                                                             ggCATCGAAGCTAACATTAAAAATGTTGATGGTGAAATTGACTGCAGCGGTAAAATGTTTGACTCGCTGAATCTAATTATTGCCGGTTTTCATGAGCCGGTTTTTGCGCCACATGACAAAGCGACCAATACACAAGCGATGATCGCCACt                                                                    >  2:63243/1‑150 (MQ=255)

                                                                             ggCATCGAAGCTAACATTAAAAATGTTGATGGTGAAATTGACTGCAGCGGTAAAATGTTTGACTCGCTGAATCTAATTATTGCCGGTTTTCATGAGCCGGTTTTTGCGCCACATAACAAAGCGACCAATACACAAGCGATGATCGCCACt                                                                    >  2:468598/1‑150 (MQ=255)

                                                                                aTCGAAGCTAACATTAAAAATGTTGATGGTGAAATTGACTGCAGCGGTAAAATGTTTGACTCGCTGAATCTAATTATTGCCGGTTTTCATGAGCCGGTTTTTGCGCCACATGACAAAGCGACCAATACACAAGCGATGATCGCCACTATCg                                                                >  1:200854/1‑151 (MQ=255)

                                                                                  cGAAGCTAACATTAAAAATGTTGATGGTGAAATTGACTGCAGCGGTAAAATGTTTGACTCGCTGAATCTAATTATTGCCGGTTTTCATGAGCCGGTTTTTGCGCCACATGACAAAGCGACCAATACACAAGCGATGATCGCCACTATCGcc                                                              <  1:284849/151‑1 (MQ=255)

                                                                                        tAACATTAAAAATGTTGATGGTGAAATTGACTGCAGCGGTAAAATGTTTGACTCGCTGAATCTAATTATTGCCGGTTTTCATGAGCCGGTTTTTGCGCCACATGACAAAGCGACCAATACACAAGCGATGATCGCCACTATCGCCAGCGGc                                                        >  2:552217/1‑151 (MQ=255)

                                                                                        tAACATTAAAAATGTTGATGGTGAAATTGACTGCAGCGGTAAAATGTTTGACTCGCTGAATCTAATTATTGCCGGTTTTCATGAGCCGGTTTTTGCGCCACATGACAAAGCGACCAATACACAAGCGATGATCGCCACTATCGCCAGCGGc                                                        >  1:196292/1‑151 (MQ=255)

                                                                                        tAACATTAAAAATGTTGATGGTGAAATTGACTGCAGCGGTAAAATGTTTGACTCGCTGAATCTAATTATTGCCGGTTTTCATGAGCCGGTTTTTGCGCCACATGACAAAGCGACCAATACACAAGCGATGATCGCCACTATCGCCAGCGGc                                                        >  1:169590/1‑151 (MQ=255)

                                                                                         aaCATTAAAAATGTTGATGGTGAAATTGACTGCAGCGGTAAAATGTTTGACTCGCTGAATCTAATTATTGCCGGTTTTCATGAGCCGGTTTTTGCGCCACATGACAAAGCGACCAATACACAAGCGATGATCGCCACTATCGCCAGCgg                                                         <  2:20118/149‑1 (MQ=255)

                                                                                         aaCATTAAAAATGTTGATGGTGAAATTGACTGCAGCGGTAAAATGTTTGACTCGCTGAATCTAATTATTGCCGGTTTTCATGAGCCGGTTTTTGCGCCACATGACAAAGCGACCAATACACAAGCGATGATCGCCACTATCGCCAGCgg                                                         <  1:228008/149‑1 (MQ=255)

                                                                                                 aaaTGTTGATGGTGAAATTGACTGCAGCGGTAAAATGTTTGACTCGCTGAATCTAATTATTGCCGGTTTTCATGAGCCGGTTTTTGCGCCACATGACAAAGCGACCAATACACAAGCGATGATCGCCACTATCGCCAGCGGCAATGTGCat                                               >  2:548870/1‑151 (MQ=255)

                                                                                                     gTTGATGGTGAAATTGACTGCAGCGGTAAAATGTTTGACTCGCTGAATCTAATTATTGCCGGTTTTCATGAGCCGGTTTTTGCGCCACATGACAAAGCGACCAATACACAAGCGATGATCGCCACTATCGCCAGCGGCAATGTGCAtata                                            <  2:304348/150‑1 (MQ=255)

                                                                                                         aTGGTGAAATTGACTGCAGCGGTAAAATGTTTGACTCGCTGAATCTAATTATTGCCGGTTTTCATGAGCCGGTTTTTGCGCCACATGACAAAGCGACCAATACACAAGCGATGATCGCCACTATCGCCAGCGGCAATGTGCATATAATAAg                                       <  2:245948/151‑1 (MQ=255)

                                                                                                             tGAAATTGACTGCAGCGGTAAAATGTTTGACTCGCTGAATCTAATTATTGCCGGTTTTCATGAGCCGGTTTTTGCGCCACATGACAAAGCGACCAATACACAAGCGATGATCGCCACTATCGCCAGCGGCAATGTGCATATAATAAGCCAt                                   >  1:579158/1‑151 (MQ=255)

                                                                                                                aaTTTACTGCAGCGGTCCAATGTTTGACTCGCTGAATCTAATTATTGCCGGTTTGCATGAGCCGGTTTTTGCGACACATGACAAAGCGACCAATACACATGAGATGACCACCCCTATAGCCAAAGGCAAAGTGCATATAATAAGCCATccc                                >  1:467908/1‑151 (MQ=255)

                                                                                                                 aTTGACTGCAGCGGTAAAATGTTTGACTCGCTGAATCTAATTATTGCCGGTTTTCATGAGCCGGTTTTTGCGCCACATGACAAAGCGACCAATACACAAGCGATGATCGCCACTATCGCCAGCGGCAATGTGCATATAATAAGCCATCCCg                               >  1:761162/1‑151 (MQ=255)

                                                                                                                      cTGCAGCGGTAAAATGTTTGACTCGCTGAATCTAATTATTGCCGGTTTTCATGAGCCGGTTTTTGCGCCACATGACAAAGCGACCAATACACAAGCGATGATCGCCACTATCGCCAGCGGCAATGTGCATATAATAAGCCATCCCGGAAAt                          >  1:115906/1‑151 (MQ=255)

                                                                                                                                  aaTGTTTGACTCGCTGAATCTAATTATTGCCGGTTTTCATGAGCCGGTTTTTGCGCCACATGACAAAGCGACCAATACACAAGCGATGATCGCCACTATCGCCAGCGGCAATGTGCATATAATAAGCCATCCCGGAAATCCCAAATATGaa              <  2:196325/151‑1 (MQ=255)

                                                                                                                                   aTGTTTGACTCGCTGAATCTAATTATTGCCGGTTTTCATGAGCCGGTTTTTGCGCCACATGACAAAGCGACCAATACACAAGCGATGATCGCCACTATCGCCAGCGGCAATGTGCATATAATAAGCCATCCCGGAAATCCCAAATATGa               >  2:634285/1‑149 (MQ=255)

                                                                                                                                          aCTCGCTGAATCTAATTATTGCCGGTTTTCATGAGCCGGTTTTTGCGCCACATGACAAAGCGACCAATACACAAGCGATGATCGCCACTATCGCCAGCGGCAATGTGCATATAATAAGCCATCCCGGAAATCCCAAATATGAAATAGAtgt      >  1:105387/1‑151 (MQ=255)

                                                                                                                                            tCGCTGAATCTAATTATTGCCGGTTTTCATGAGCCGGTTTTTGCGCCACATGACAAAGCGACCAATACACAAGCGATGATCGCCACTATCGCCAGCGGCAATGTGCATATAATAAGCCATCCCGGAAATCCCAAATATGAAATAGAtgtg     >  2:429731/1‑150 (MQ=255)

                                                                                                                                            ccGCTGAATCTACTTATTGCCGGTTTTCATGAGCCGGTGTTTGCGCCACATGACAAATCGACCAATACACAAGCGATGATCGCCACTATCGCCAGCGGCAAAGTGCATATAATAAACCATCCCGGAAATCCCAAATATGAAATAGAtgt      >  1:539546/2‑149 (MQ=255)

                                                                                                                                              gCTGAATCTAATTATTGCCGGTTTTCATGAGCCGGTTTTTGCGCCACATGACAAAGCGACCAATACACAAGCGATGATCGCCACTATCGCCAGCGGCAATGTGCATTTAATAAGCCATCCCGGAAATCCCAAATATGAAATAGATGTGaaa  <  1:460341/151‑1 (MQ=255)

AAGATGCGCCGCATCACTGGCACTTCATTAACATGCGTATCTGGCCGCGAGTGGTTGATGGGGTAGGGATCCTGCGCGGCATCGAAGCTAACATTAAAAATGTTGATGGTGAAATTGACTGCAGCGGTAAAATGTTTGACTCGCTGGATCTAATTATTGCCGGTTTTCATGAGCCGGTTTTTGCGCCACATGACAAAGCGACCAATACACAAGCGATGATCGCCACTATCGCCAGCGGCAATGTGCATATAATAAGCCATCCCGGAAATCCCAAATATGAAATAGATGTGAAA  >  NC_000913/1099012‑1099304

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A