Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A3 F50 I2 R1	257	49.9	1554294	99.0%	1538751	150.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,119,351	C→T	G33R (GGA→AGA)	yceO ←	uncharacterized protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,119,351	0	C	T	100.0%	164.0 / NA	43	G33R (GGA→AGA)	yceO	uncharacterized protein
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (26/17); total (26/17)

GAGATGTCTGGTGAGTGCTGTAGAAGCAGGAATAATCTCATTCAATTTTTCACGCTAAATGAAAGGGAGATTAAATATTTGTCAGTTTATGTCAGTGCGGAAAACTAAAAATAAAACACATAGATCAGATCCATAATTGCCAACAATCCCCAGAGAAAAAGATAAAGCATCAGATGCTCGCGAATATTATTGATGAGATAGTGCAACAGCCGACGCATTAAATACTCCTGTAAAAACGGCCTCATTCTGGAGGCCGTAATGATGATTATTGGAAGCGGGAAAGCCTGAATGGCGT  >  NC_000913/1119203‑1119497

gagaTGTCTGGTGAGTGCTGTAGAAGCAGGAATAATCTCATTCAATTTTTCACGCTAAATGAAAGGGAGATTAAATATTTGTCAGTTTATGTCAGTGCGGAAAACTAAAAATAAAACACATAGATCAGATCCATAATTGCCAACAATctcc                                                                                                                                                  >  1:271831/1‑151 (MQ=255)

     gTCTGGTGAGTGCTGTAGAAGCAGGAATAATCTCATTCAATTTTTCACGCTAAATGAAAGGGAGATTAAATATTTGTCAGTTTATGTCAGTGCGGAAAACTAAAAATAAAACACATAGATCAGATCCATAATTGCCAACAATCTCCagag                                                                                                                                              <  2:528404/150‑1 (MQ=255)

         ggTGAGTGCTGTAGAAGCAGGAATAATCTCATTCAATTTTTCACGCTAAATGAAAGGGAGATTAAATATTTGTCAGTTTATGTCAGTGCGGAAAACTAAAAATAAAACACATAGATCAGATCCATAATTGCCAACAATCTCCAGAGaaa                                                                                                                                           <  1:486918/149‑1 (MQ=255)

          gTGAGTGCTGTAGAAGCAGGAATAATCTCATTCAATTTTTCACGCTAAATGAAAGGGAGATTAAATATTTGTCAGTTTATGTCAGTGCGGAAAACTAAAAATAAAACACATAGATCAGATCCATAATTGCCAACAATCTCCAGAGAAAAAg                                                                                                                                        <  1:302437/151‑1 (MQ=255)

          gTGAGTGCTGTAGAAGCAGGAATAATCTCATTCAATTTTTCACGCTAAATGAAAGGGAGATTAAATATTTGTCAGTTTATGTCAGTGCGGAAAACTAAAAATAAAACACATAGATCAGATCCATAATTGCCAACAATCTCCAGAGAAAAAg                                                                                                                                        <  2:365722/151‑1 (MQ=255)

                gCTGTAGAAGCAGGAATAATCTCATTCAATTTTTCACGCTAAATGAAAGGGAGATTAAATATTTGTCAGTTTATGTCAGTGCGGAAAACTAAAAATAAAACACATAGATCAGATCCATAATTGCCAACAATCTCCAGAGAAAAAGATaaa                                                                                                                                   <  2:7502/150‑1 (MQ=255)

                gCTGTAGAAGCAGGAATAATCTCATTCAATTTTTCACGCTAAATGAAAGGGAGATTAAATATTTGTCAGTTTATGTCAGTGCGGAAAACTAAAAATAAAACACATAGATCAGATCCATAATTGCCAACAATCTCCAGAGAAAAAGATaaa                                                                                                                                   <  2:650459/150‑1 (MQ=255)

                   gTAGAAGCAGGAATAATCTCATTCAATTTTTCACGCTAAATGAAAGGGAGATTAAATATTTGTCAGTTTATGTCAGTGCGGAAAACTAAAAATAAAACACATAGATCAGATCCATAATTGCCAACAATCTCCAGAGAAAAAGATAAAGCAt                                                                                                                               >  1:545690/1‑151 (MQ=255)

                   gTAGAAGCAGGAATAATCTCATTCAATTTTTCACGCTAAATGAAAGGGAGATTAAATATTTGTCAGTTTATGTCAGTGCGGAAAACTAAAAATAAAACACATAGATCAGATCCATAATTGCCAACAATCTCCAGAGAAAAAGATAAAGCAt                                                                                                                               <  2:678694/151‑1 (MQ=255)

                    tAGAAGCAGGAATAATCTCATTCAATTTTTCACGCTAAATGAAAGGGAGATTAAATATTTGTCAGTTTATGTCAGTGCGGAAAACTAAAAATAAAACACATAGATCAGATCCATAATTGCCAACAATCTCCAGAGAAAAAGATAAAGCATc                                                                                                                              >  2:603258/1‑151 (MQ=255)

                          caagAATAATCTCATTCAATTTTTCACGCTAAATGAAAGGGAGATTAAATATTTGTCAGTTTATGTCAGTGCGGAAAACTAAAAATAAAACACATAGATCAGATCCATAATTGCCAACAATCTCCAGAGAAAAAGATAAAGCATCAGATGc                                                                                                                        >  1:324067/4‑151 (MQ=255)

                              aataatCTCATTCAATTTTTCACGCTAAATGAAAGGGAGATTAAATATTTGTCAGTTTATGTCAGTGCGGAAAACTAAAAATAAAACACATAGATCAGATCCATAATTGCCAACAATCTCCAGAGAAAAAGATAAAGCATCAGATGCTcgc                                                                                                                    >  1:459831/1‑151 (MQ=255)

                                   tctcATTCAATTTTTCACGCTAAATGAAAGGGAGATTAAATATTTGTCAGTTTATGTCAGTGCGGAAAACTAAAAATAAAACACATAGATCAGATCCATAATTGCCAACAATCTCCAGAGAAAAAGATAAAGCATCAGATGCTCGCGAat                                                                                                                >  2:775757/1‑150 (MQ=255)

                                      cATTCAATTTTTCACGCTAAATGAAAGGGAGATTAAATATTTGTCAGTTTATGTCAGTGCGGAAAACTAAAAATAAAACACATAGATCAGATCCATAATTGCCAACAATCTCCAGAGAAAAAGATAAAGCATCAGATGCTCGCGAAtatt                                                                                                             <  2:157797/150‑1 (MQ=255)

                                       aTTCAATTTTTCACGCTAAATGAAAGGGAGATTAAATATTTGTCAGTTTATGTCAGTGCGGAAAACTAAAAATAAAACACATAGATCAGATCCATAATTGCCAACAATCTCCAGAGAAAAAGATAAAGCATCAGATGCTCGCGAAtatta                                                                                                            <  2:543523/150‑1 (MQ=255)

                                         tCAATTTTTCACGCTAAATGAAAGGGAGATTAAATATTTGTCAGTTTATGTCAGTGCGGAAAACTAAAAATAAAACACATAGATCAGATCCATAATTGCCAACAATCTCCAGAGAAAAAGATAAAGCATCAGATGCTCGCGAATATTATtg                                                                                                         >  1:44602/1‑151 (MQ=255)

                                              ttttCACGCTAAATGAAAGGGAGATTAAATATTTGTCAGTTTATGTCAGTGCGGAAAACTAAAAATAAAACACATAGATCAGATCCATAATTGCCAACAATCTCCAGAGAAAAAGATAAAGCATCAGATGCTCGCGAATATTATtgatg                                                                                                      >  2:156560/1‑149 (MQ=255)

                                                      cTAAATGAAAGGGAGATTAAATATTTGTCAGTTTATGTCAGTGCGGAAAACTAAAAATAAAACACATAGATCAGATCCATAATTGCCAACAATCTCCAGAGAAAAAGATAAAGCATCAGATGCTCGCGAATATTATTGATGAGATAGTGc                                                                                             >  1:461465/1‑150 (MQ=255)

                                                            gAAAGGGAGATTAAATATTTGTCAGTTTATGTCAGTGCGGAAAACTAAAAATAAAACACATAGATCAGATCCATAATTGCCAACAATCTCCAGAGAAAAAGATAAAGCATCAGATGCTCGCGAATATTATTGATGAGATAGTGCAACAGcc                                                                                      <  1:507547/151‑1 (MQ=255)

                                                            gAAAGGGAGATTAAATATTTGTCAGTTTATGTCAGTGCGGAAAACTAAAAATAAAACACATAGATCAGATCCATAATTGCCAACAATCTCCAGAGAAAAAGATAAAGCATCAGATGCTCGCGAATATTATTGATGAGATAGTGCAACAGcc                                                                                      <  1:775681/151‑1 (MQ=255)

                                                             aaaGGGAGATTAAATATTTGTCAGTTTATGTCAGTGCGGAAAACTAAAAATAAAACACATAGATCAGATCCATAATTGCCAACAATCTCCAGAGAAAAAGATAAAGCATCAGATGCTCGCGAATATTATTGATGAGATAGTGCAACAGCCg                                                                                     >  2:96160/1‑151 (MQ=255)

                                                                 ggAGATTAAATATTTGTCAGTTTATGTCAGTGCGGAAAACTAAAAATAAAACACATAGATCAGATCCATAATTGCCAACAATCTCCAGAGAAAAAGATAAAGCATCAGATGCTCGCGAATATTATTGATGAGATAGTGCAACAGCCGACGc                                                                                 >  2:350411/1‑151 (MQ=255)

                                                                   agaTTAAATATTTGTCAGTTTATGTCAGTGCGGAAAACTAAAAATAAAACACATAGATCAGATCCATAATTGCCAACAATCTCCAGAGAAAAAGATAAAGCATCAGATGCTCGCGAATATTATTGATGAGATAGTGCAACAGCCGACGCAt                                                                               >  1:375227/1‑151 (MQ=255)

                                                                       tAAATATTTGTCAGTTTATGTCAGTGCGGAAAACTAAAAATAAAACACATAGATCAGATCCATAATTGCCAACAATCTCCAGAGAAAAAGATAAAGCATCAGATGCTCGCGAATATTATTGATGAGATAGTGCAACAGCCGACGCATTaa                                                                            >  2:16396/1‑150 (MQ=255)

                                                                        aaaTATTTGTCAGTTTATGTCAGTGCGGAAAACTAAAAATAAAACACATAGATCAGATCCATAATTGCCAACAATCTCCAGAGAAAAAGATAAAGCATCAGATGCTCGCGAATATTATTGATGAGATAGTGc                                                                                             >  2:70533/1‑132 (MQ=255)

                                                                        aaaTATTTGTCAGTTTATGTCAGTGCGGAAAACTAAAAATAAAACACATAGATCAGATCCATAATTGCCAACAATCTCCAGAGAAAAAGATAAAGCATCAGATGCTCGCGAATATTATTGATGAGATAGTGc                                                                                             <  1:70502/132‑1 (MQ=255)

                                                                               tGTCAGTTTATGTCAGTGCGGAAAACTAAAAATAAAACACATAGATCAGATCCATAATTGCCAACAATCTCCAGAGAAAAAGATAAAGCATCAGATGCTCGCGAATATTATTGATGAGATAGTGCAACAGCCGACGCATTAAATACTCCTg                                                                   >  2:206732/1‑151 (MQ=255)

                                                                               tGTCAGTTTATGTCAGTGCGGAAAACTAAAAATAAAACACATAGATCAGATCCATAATTGCCAACAATCTCCAGAGAAAAAGATAAAGCATCAGATGCTCGCGAATATTATTGATGAGATAGTGCAACAGCCGACGCATTAAATACCCCTg                                                                   >  1:632110/1‑151 (MQ=255)

                                                                                    gTTTATGTCAGTGCGGAAAACTAAAAATAAAACACATAGATCAGATCCATAATTGCCAACAATCTCCAGAGAAAAAGATAAAGCATCAGATGCTCGCGAATATTATTGATGAGATAGTGCAACAGCCGACGCATTAAATACTCCTGTaaa                                                               >  2:671645/1‑150 (MQ=255)

                                                                                     tttATGTCAGTGCGGAAAACTAAAAATAAAACACATAGATCAGATCCATAATTGCCAACAATCTCCAGAGAAAAAGATAAAGCATCAGATGCTCGCGAATATTATTGATGAGATAGTGCAACAGCCGACGCATTAAATACTCCTGTaaaa                                                              >  1:305965/1‑150 (MQ=255)

                                                                                        aTGTCAGTGCGGAAAACTAAAAATAAAACACATAGATCAGATCCATAATTGCCAACAATCTCCAGAGAAAAAGATAAAGCATCAGATGCTCGCGAATATTATTGATGAGATAGTGCAACAGCCGACGCATTAAATACTCCTGTAAAAACgg                                                          >  2:366650/1‑151 (MQ=255)

                                                                                            cAGTGCGGAAAACTAAAAATAAAACACATAGATCAGATCCATAATTGCCAACAATCTCCAGAGAAAAAGATAAAGCATCAGATGCTCGCGAATATTATTGATGAGATAGTGCAACAGCCGACGCATTAAATACTCCTGTAAAAACGGCCTc                                                      <  2:332377/151‑1 (MQ=255)

                                                                                             aGTGCGGAAAACTAAAAATAAAACCCATAGATCAGATCCATAATTGCCAACAATCTCCAGAGAAAAAGATAAAGCATCAGATGCTCGCGAATATTATTGATGAGATAGTGCAACAGCCGACGCATTAAATACTCCTGTAAAAACGGCCTCa                                                     <  2:250651/151‑1 (MQ=255)

                                                                                                   gAAAACTAAAAATAAAACACATAGATCAGATCCATAATTGCCAACAATCTCCAGAGAAAAAGCTAAAGCATCAGATACTCGCGACTATTATTGCTGAGATAGTGCAACAGCCGACGCATTAAATACTCCTGTAAAAACGGCCTCATTCTg                                                >  2:570158/1‑150 (MQ=255)

                                                                                                      aaCTAAAAATAAAACACATAGATCAGATCCATAATTGCCAACAATCTCCAGAGAAAAAGATAAAGCATCAGATGCTCGCGAATATTATTGATGAGATAGTGCAACAGCCGACGCATTAAATACTCCTGTAAAAACGGCCTCATTCTGGAgg                                            >  2:43575/1‑151 (MQ=255)

                                                                                                       aCTAAAAATAAAACACATAGATCAGATCCATAATTGCCAACAATCTCCAGAGAAAAAGATAAAGCATCAGATGCTCGCGAATATTATTGATGAGATAGTGCAACAGCCGACGCATTAAATACTCCTGTAAAAACGGCCTCATTCTGGAGGc                                           >  1:464956/1‑151 (MQ=255)

                                                                                                                           atcagatcCATAATTGCCAACAATCTCCAGAGAAAAAGATAAAGCATCAGATGCTCGCGAATATTATTGATGAGATAGTGCAACAGCCGACGCATTAAATACTCCTGTAAAAACGGCCTCATTCTGGAGGCCGTAATGATGATTATTGGa                        >  2:500876/1‑150 (MQ=255)

                                                                                                                                atcCATAATTGCCAACAATCTCCAGAGAAAAAGATAAAGCATCAGATGCTCGCGAATATTATTGATGAGATAGTGCAACAGCCGACGCATTAAATACTCCTGTAAAAACGGCCTCATTCTGGAGGCCGTAATGATGATTATTGGAAGCggg                  <  2:346555/151‑1 (MQ=255)

                                                                                                                                  ccATAATTGCCAACAATCTCCAGAGAAAAAGATAAAGCATCAGATGCTCGCGAATATTATTGATGAGATAGTGCAACAGCCGACGCATTAAATACTCCTGTAAAAACGGCCTCATTCTGGAGGCCGTAATGATGATTATTGGAAGCGGGa                 >  2:68083/1‑150 (MQ=255)

                                                                                                                                   cATAATTGCCAACAATCTCCAGAGAAAAAGATAAAGCATCAGATGCTCGCGAATATTATTGATGAGATAGTGCAACAGCCGACGCATTAAATACTCCTGTAAAAACGGCCTCATTCTGGAGGCCGTAATGATGATTATTGGAAGCGGGaa                >  1:584285/1‑150 (MQ=255)

                                                                                                                                    aTAATTGCCAACAATCTCCAGAGAAAAAGATAAAGCATCAGATGCTCGCGAATATTATTGATGAGATAGTGCAACAGCCGACGCATTAAATACTCCTGTAAAAACGGCCTCATTCTGGAGGCCGTAATGATGATTATTGGAAGCGGGaa                >  2:653529/1‑149 (MQ=255)

                                                                                                                                        ttGCCAACAATCTCCAGAGAAAAAGATAAAGCATCAGATGCTCGCGAATATTATTGATGAGATAGTGCAACAGCCGACGCATTAAATACTCCTGTAAAAACGGCCTCATTCTGGAGGCCGTAATGATGATTATTGGAAGCGGGAAAGCCTg          >  2:57884/1‑151 (MQ=255)

                                                                                                                                          gCCAACAATCTCCAGAGAAAAAGATAAAGCATCAGATGCTCGCGAATATTATTGATGAGATAGTGCAACAGCCGACGCATTAAATACTCCTGTAAAAACGGCCTCATTCTGGAGGCCGTAATGATGATTATTGGAAGCGGGAAAGCCTGa         <  1:79080/150‑1 (MQ=255)

                                                                                                                                                aaTCTCCAGAGAAAAAGATAAAGCATCAGATGCTCGCGAATATTATTGATGAGATAGTGCAACAGCCGACGCATTAAATACTCCTGTAAAAACGGCCTCATTCTGGAGGCCGTAATGATGATTATTGGAAGCGGGAAAGCCTGAATGGCGt  <  2:442891/151‑1 (MQ=255)

GAGATGTCTGGTGAGTGCTGTAGAAGCAGGAATAATCTCATTCAATTTTTCACGCTAAATGAAAGGGAGATTAAATATTTGTCAGTTTATGTCAGTGCGGAAAACTAAAAATAAAACACATAGATCAGATCCATAATTGCCAACAATCCCCAGAGAAAAAGATAAAGCATCAGATGCTCGCGAATATTATTGATGAGATAGTGCAACAGCCGACGCATTAAATACTCCTGTAAAAACGGCCTCATTCTGGAGGCCGTAATGATGATTATTGGAAGCGGGAAAGCCTGAATGGCGT  >  NC_000913/1119203‑1119497

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A