Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A3 F50 I2 R1	257	49.9	1554294	99.0%	1538751	150.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,151,418	A→G	intergenic (+14/‑197)	fabG → / → acpP	3‑oxoacyl‑[acyl‑carrier‑protein] reductase/acyl carrier protein (ACP)

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,151,418	0	A	G	100.0%	157.0 / NA	43	intergenic (+14/‑197)	fabG/acpP	3‑oxoacyl‑[acyl‑carrier‑protein] reductase/acyl carrier protein (ACP)
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (19/24); total (19/24)

GCAGGTTCCTGCGGGTCGCCTCGGCGGCGCACAGGAAATCGCCAACGCGGTTGCATTCCTGGCATCCGACGAAGCAGCTTACATCACGGGTGAAACTTTGCATGTGAACGGCGGGATGTACATGGTCTGACCGCGATTTGCACAAAATGCTCATGTTGCGCGCAGTCTGCGTGGTTATGAGTAATAATTAGTGCAAAATGATTTGCGTTATTGGGGGGTAAGGCCTCAAAATAACGTAAAATCGTGGTAAGACCTGCCGGGATTTAGTTGCAAATTTTTCAACATTTTATA  >  NC_000913/1151275‑1151565

gcAGGTTCCTGCGGGTCGCCTCGGCGGCGCACAGGAAATCGCCAACGCGGTTGCATTCCTGGCATCCGACGAAGCAGCTTACATCACGGGTGAAACTTTGCATGTGAACGGCGGGATGTACATGGTCTGACCGCGATTTGCACGAAATGCt                                                                                                                                              <  2:243015/151‑1 (MQ=255)

 cAGGTTCCTGCGGGTCGCCTCGGCGGCGCACAGGAAATCGCCAACGCGGTTGCATTCCTGGCATCCGACGAAGCAGCTTACATCACGGGTGAAACTTTGCATGTGAACGGCGGGATGTACATGGTCTGACCGCGATTTGCACGAAATGCTc                                                                                                                                             <  2:435551/151‑1 (MQ=255)

     ttCCTGCGGGTCGCCTCGGCGGCGCACAGGAAATCGCCAACGCGGTTGCATTCCTGGCATCCGACGAAGCAGCTTACATCACGGGTGAAACTTTGCATGTGAACGGCGGGATGTACATGGTCTGACCGCGATTTGCACGAAATGCTCATg                                                                                                                                          >  2:725055/1‑150 (MQ=255)

          gCGGGTCGCCTCGGCGGCGCACAGGAAATCGCCAACGCGGTTGCATTCCTGGCATCCGACGAAGCAGCTTACATCACGGGTGAAACTTTGCATGTGAACGGCGGGATGTACATGGTCTGACCGCGATTTGCACGAAATGCTCATGTTgc                                                                                                                                      <  1:325994/149‑1 (MQ=255)

              atCGCCACGACGGCGCACAGGAAATCGCCAACGCGGTTGCATTCCTGGCATCCGACGAAGCAGCTTACATCACGGGTGAAACTTTGCATGTGAACGGCGGGATGTACAAGGTCTGACCCGGATTTGCACGAAATGCTCATGTTgcg                                                                                                                                     >  1:374833/2‑146 (MQ=255)

                  ccTCGGCGGCGCACAGGAAATCGCCAACGCGGTTGCATTCCTGGCATCCGACGAAGCAGCTTACATCACGGGTGAAACTTTGCATGTGAACGGCGGGATGTACATGGTCTGACCGCGATTTGCACGAAATGCTCATGTTGCGCGCAGTCTg                                                                                                                            >  2:693509/1‑151 (MQ=255)

                        cggcgCACAGGAAATCGCCAACGCGGTTGCATTCCTGGCATCCGACGAAGCAGCTTACATCACGGGTGAAACTTTGCATGTGAACGGCGGGATGTACATGGTCTGACCGCGATTTGCACGAAATGCTCATGTTGCGCGCAGTCTGCGTGGt                                                                                                                      >  2:11483/1‑151 (MQ=255)

                           cgcACAGGAAATCGCCAACGCGGTTGCATTCCTGGCATCCGACGAAGCAGCTTACATCACGGGTGAAACTTTGCATGTGAACGGCGGGATGTACATGGTCTGACCGCGATTTGCACGAAATGCTCATGTTGCGCGCAGTCTGCGTGGTTAt                                                                                                                   <  2:242070/151‑1 (MQ=255)

                            gcACAGGAAATCGCCAACGCGGTTGCATTCCTGGCATCCGACGAAGCAGCTTACATCACGGGTGAAACTTTGCATGTGAACGGCGGGATGTACATGGTCTGACCGCGATTTGCACGAAATGCTCATGTTGCGCGCAGTCTGCGTGGTTATg                                                                                                                  <  2:46097/151‑1 (MQ=255)

                            gcACAGGAAATCGCCAACGCGGTTGCATTCCTGGCATCCGACGAAGCAGCTTACATCACGGGTGAAACTTTGCATGTGAACGGCGGGATGTACATGGTCTGACCGCGATTTGCACGAAATGCTCATGTTGCGCGCAGTCTGCGTGGTTATg                                                                                                                  <  1:176171/151‑1 (MQ=255)

                                aGGAAATCGCCAACGCGGTTGCATTCCTGGCATCCGACGAAGCAGCTTACATCACGGGTGAAACTTTGCATGTGAACGGCGGGATGTACATGGTCTGACCTCGATTTGCACGAAATGCTCATGTTGCGCGCAGTCTGCGTGGTTATGAGta                                                                                                              <  1:747853/151‑1 (MQ=255)

                                 ggAAATCGCCAACGCGGTTGCATTCCTGGCATCCGACGAAGCAGCTTACATCACGGGTGAAACTTTGCATGTGAACGGCGGGATGTACATGGTCTGACCGCGATTTGCACGAAATGCTCATGTTGCGCGCAGTCTGCGTGGTTATGAGtaa                                                                                                             <  1:243063/151‑1 (MQ=255)

                                 ggAAATCGCCAACGCGGTTGCATTCCTGGCATCCGACGAAGCAGCTTACATCACGGGTGAAACTTTGCATGTGAACGGCGGGATGTACATGGTCTGACCGCGATTTGCACGAAATGCTCATGTTGCGCGCAGTCTGCGTGGTTATGAGtaa                                                                                                             <  2:585234/151‑1 (MQ=255)

                                   aaaTCGCCAACGCGGTTGCATTCCTGGCATCCGACGAAGCAGCTTACATCACGGGTGAAACTTTGCATGTGAACGGCGGGATGTACATGGTCTGACCGCGATTTGCACGAAATGCTCATGTTGCGCGCAGTCTGCGTGGTTATGAGtaata                                                                                                           <  1:348965/151‑1 (MQ=255)

                                   aaaTCGCCAACGCGGTTGCATTCCTGGCATCCGACGAAGCAGCTTACATCACGGGTGAAACTTTGCATGTGAACGGCGGGATGTACATGGTCTGACCGCGATTTGCACGAAATGCTCATGTTGCGCGCAGTCTGCGTGGTTATGAGtaat                                                                                                            >  1:595936/1‑150 (MQ=255)

                                        gCCAACGCGGTTGCATTCCTGGCATCCGACGAAGCAGCTTACATCACGGGTGAAACTTTGCATGTGAACGGCGGGATGTACATGGTCTGACCGCGATTTGCACGAAATGCTCATGTTGCGCGCAGTCTGCGTGGTTATGAGTAATAAtt                                                                                                        >  2:567890/1‑149 (MQ=255)

                                           aaCGCGGTTGCATTCCTGGCATCCGACGAAGCAGCTTACATCACGGGTGAAACTTTGCATGTGAACGGCGGGATGTACATGGTCTGACCGCGATTTGCACGAAATGCTCATGTTGCGCGCAGTCTGCGTGGTTATGAGTAATAATTAGTg                                                                                                    >  1:294008/1‑150 (MQ=255)

                                                    gCATTCCTGGCATCCGACGAAGCAGCTTACATCACGGGTGAAACTTTGCATGTGAACGGCGGGATGTACATGGTCTGACCGCGATTTGCACGAAATGCTCATGTTGCGCGCAGTCTGCGTGGTTATGAGTAATAATTAGTGCAAAATGAt                                                                                           <  2:294819/150‑1 (MQ=255)

                                                    gCATTCCTGGCATCCGACGAAGCAGCTTACATCACGGGTGAAACTTTGCATGTGAACGGCGGGATGTACATGGTCTGACCGCGATTTGCACGAAATGCTCATGTTGCGCGCAGTCTGCGTGGTTATGAGTAATAATTAGTGCAAAATGAt                                                                                           <  1:481114/150‑1 (MQ=255)

                                                    gCATTCCTGGCATCCGACGAAGCAGCTTACATCACGGGTGAAACTTTGCATGTGAACGGCGGGATGTACATGGTCTGACCGCGATTTGCACGAAATGCTCATGTTGCGCGCAGTCTGCGTGGTTATGAGTAATAATTAGTGCAAAATGAt                                                                                           <  1:675334/150‑1 (MQ=255)

                                                          cTGGCATCCGACGAAGCAGCTTACATCACGGGTGAAACTTTGCATGTGAACGGCGGGATGTACATGGTCTGACCGCGATTTGCACGAAATGCTCATGTTGCGCGCAGTCTGCGGGGTTATGAGTAATAATTAGTGCAAAATGATTTGCGtt                                                                                    >  1:323744/1‑151 (MQ=255)

                                                              cATCCGACGAAGCAGCTTACATCACGGGTGAAACTTTGCATGTGAACGGCGGGATGTACATGGTCTGACCGCGATTTGCACGAAATGCTCATGTTGCGCGCAGTCTGCGTGGTTATGAGTAATAATTAGTGCAAAATGATTTGCGTTATTg                                                                                <  2:282748/151‑1 (MQ=255)

                                                               aTCCGACGAAGCAGCTTACATCACGGGTGAAACTTTGCATGTGAACGGCGGGATGTACATGGTCTGACCGCGATTTGCACGAAATGCTCATGTTGCGCGCAGTCTGCGTGGTTATGAGTAATAATTAGTGCAAAATGATTTGCGTTATTgg                                                                               <  1:17664/151‑1 (MQ=255)

                                                               aTCCGACGAAGCAGCTTACATCACGGGTGAAACTTTGCATGTGAACGGCGGGATGTACATGGTCTGACCGCGATTTGCACGAAATGCTCATGTTGCGCGCAGTCTGCGTGGTTATGAGTAATAATTAGTGCAAAATGATTTGCGTTATTgg                                                                               <  2:25853/151‑1 (MQ=255)

                                                                  cgacgaAGCAGCTTACATCACGGGTGAAACTTTGCATGTGAACGGCGGGATGTACATGGTCTGACCGCGATTTGCACGAAATGCTCATGTTGCGCGCAGTCTGCGTGGTTATGAGTAATAATTAGTGCAAAATGATTTGCGTTATTggggg                                                                            <  2:112813/151‑1 (MQ=255)

                                                                   gacgaAGCAGCTTACATCACGGGTGAAACTTTGCATGTGAACGGCGGGATGTACATGGTCTGACCGCGATTTGCACGAAATGCTCATGTTGCGCGCAGTCTGCGTGGTTATGAGTAATAATTAGTGCAAAATGATTTGCGTTATTgggggg                                                                           >  2:306403/1‑151 (MQ=255)

                                                                          cagcTTACATCACGGGTGAAACTTTGCATGTGAACGGCGGGATGTACATGGTCTGACCGCGATTTGCACGAAATGCTCATGTTGCGCGCAGTCTGCGTGGTTATGAGTAATAATTAGTGCAAAATGATTTGCGTTATTGGGGGGTAAGGcc                                                                    <  2:706552/151‑1 (MQ=255)

                                                                           agcTTACATCACGGGTGAAACTTTGCATGTGAACGGCGGGATGTACATGGTCTGACCGCGATTTGCACGAAATGCTCATGTTGCGCGCAGTCTGCGTGGTTATGAGTAATAATTAGTGCAAAATGATTTGCGTTATTGGGGGGTAAGGCCt                                                                   <  2:357128/151‑1 (MQ=255)

                                                                                  aTCACGGGTGAAACTTTGCATGTGAACGGCGGGATGTACATGGTCTGACCGCGATTTGCACGAAATGCTCATGTTGCGCGCAGTCTGCGTGGTTATGAGTAATAATTAGTGCAAAATGATTTGCGTTATTGGGGGGTAAGGCCTCAAAAt                                                             >  1:37201/1‑150 (MQ=255)

                                                                                    cACGGGTGAAACTTTGCATGTGAACGGCGGGATGTACATGGTCTGACCGCGATTTGCACGAAATGCTCATGTTGCGCGCAGTCTGCGTGGTTATGAGTAATAATTAGTGCAAAATGATTTGCGTTATTGGGGGGTAAGGCCTCAAAATAAc                                                          <  2:220410/151‑1 (MQ=255)

                                                                                       gggTGAAACTTTGCATGTGAACGGCGGGATGTACATGGTCTGACCGCGATTTGCACGAAATGCTCATGTTGCGCGCAGTCTGCGTGGTTATGAGTAATAATTAGTGCAAAATGATTTGCGTTATTGGGGGGTAAGGCCTCAAAATAACGTa                                                       <  1:121459/151‑1 (MQ=255)

                                                                                          tGAAACTTTGCATGTGAACGGCGGGATGTACATGGTCTGACCGCGATTTGCACGAAATGCTCATGTTGCGCGCAGTCTGCGTGGTTATGAGTAATAATTAGTGCAAAATGATTTGCGTTATTGGGGGGTAAGGCCTCAAAATAACGTaaa                                                     >  2:138631/1‑150 (MQ=255)

                                                                                             aaCTTTGCATGTGAACGGCGGGATGTACATGGTCTGACCGCGATTTGCACGAAATGCTCATGTTGCGCGCAGTCTGCGTGGTTATGAGTAATAATTAGTGCAAAATGATTTGCGTTATTGGGGGGTAAGGCCTCAAAATAACGTAAAATCg                                                 >  2:456154/1‑151 (MQ=255)

                                                                                              aCTTTGCATGTGAACGGCGGGATGTACATGGTCTGACCGCGATTTGCACGAAATGCTCATGTTGCGCGCAGTCTGCGTGGTTATGAGTAATAATTAGTGCAAAATGATTTGCGTTATTGGGGGGTAAGGCCTCAAAATAACGTAAAATCGt                                                <  2:472837/151‑1 (MQ=255)

                                                                                              aCTTTGCATGTGAACGGCGGGATGTACATGGTCTGAACGCGATTTGCACGAAATGCTCATGTTGCGCGCAGTCTGCGTGGTTATGAGTAATAATTAGTGCAAAATGATTTGCGTTATTGGGGGGTAAGGCCTCAAAATAACGTAAAATCg                                                 >  2:365186/1‑150 (MQ=255)

                                                                                                 ttGCATGTGAACGGCGGGATGTACATGGTCTGACCGCGATTTGCACGAAATGCTCATGTTGCGCGCAGTCTGCGTGGTTATGAGTAATAATTAGTGCAAAATGATTTGCGTTATTGGGGGGTAAGGCCTCAAAATAACGTAAAATCGTg                                               <  1:636246/149‑1 (MQ=255)

                                                                                                             agcggGATGTACATGGTCTGACCGCGATTTGCACGAAATGCTCATGTTGCGCGCAGTCTGCGTGGTTATGAGTAATAATTAGTGCAAAATGATTTGCGTTATTGGGGGGTAAGGCCTCAAAATAACGTAAAATCGTGGTAAGACCTGCCgg                                 >  1:191433/2‑151 (MQ=255)

                                                                                                                gggATGTACATGGTCTGACCGCGATTTGCACGAAATGCTCATGTTGCGCGCAGTCTGCGTGGTTATGAGTAATAATTAGTGCAAAATGATTTGCGTTATTGGGGGGTAAGGCCTCAAAATAACGTAAAATCGTGGTAAGACCTGCCGGGAt                              >  1:156866/1‑151 (MQ=255)

                                                                                                                 ggATGTACATGGTCTGACCGCGATTTGCACGAAATGCTCATGTTGCGCGCAGTCTGCGTGGTTATGAGTAATAATTAGTGCAAAATGATTTGCGTTATTGGGGGGTAAGGCCTCAAAATAACGTAAAATCGTGGTAAGACCTGCCGGGAtt                             >  2:269970/1‑151 (MQ=255)

                                                                                                                   aTGTACATGGTCTGACCGCGATTTGCACGAAATGCTCATGTTGCGCGCAGTCTGCGTGGTTATGAGTAATAATTAGTGCAAAATGATTTGCGTTATTGGGGGGTAAGGCCTCAAAATAACGTAAAATCGTGGTAAGACCTGCCGGGAttt                            >  1:361228/1‑150 (MQ=255)

                                                                                                                                gACCGCGATTTGCACGAAATGCTCATGTTGCGCGCAGTCTGCGTGGTTATGAGTAATAATTAGTGCAAAATGATTTGCGTTATTGGGGGGTAAGGCCTCAAAATAACGTAAAATCGTGGTAAGACCTGCCGGGATTTAGTTGCAAAtttt               <  2:189282/150‑1 (MQ=255)

                                                                                                                                  ccGCGATTTGCACGAAATGCTCATGTTGCGCGCAGTCTGCGTGGTTATGAGTAATAATTAGTGCAAAATGATTTGCGTTATTGGGGGGTAAGGCCTCAAAATAACGTAAAATCGTGGTAAGACCTGCCGGGATTTAGTTGCAAATTTTTc             >  1:297120/1‑150 (MQ=255)

                                                                                                                                  ccGCGATTTGCACGAAATGCTCATGTTGCGCGCAGTCTGCGTGGTTATGAGTAATAATTAGTGCAAAATGATTTGCGTTATTGGGGGGTAAGGCCTCAAAATAACGTAAAATCGTGGTAAGACCTGCCGGGATTTAGTTGCAAATTTTTc             >  1:8480/1‑150 (MQ=255)

                                                                                                                                            cacgAAATGCTCATGTTGCGCGCAGTCTGCGTGGTTATGAGTAATAATTAGTGCAAAATGATTTGCGTTATTGGGGGGTAAGGCCTCAAAATAACGTAAAATCGTGGTAAGACCTGCCGGGATTTAGTTGCAAATTTTTCAACATTTtata  <  1:443201/151‑1 (MQ=255)

GCAGGTTCCTGCGGGTCGCCTCGGCGGCGCACAGGAAATCGCCAACGCGGTTGCATTCCTGGCATCCGACGAAGCAGCTTACATCACGGGTGAAACTTTGCATGTGAACGGCGGGATGTACATGGTCTGACCGCGATTTGCACAAAATGCTCATGTTGCGCGCAGTCTGCGTGGTTATGAGTAATAATTAGTGCAAAATGATTTGCGTTATTGGGGGGTAAGGCCTCAAAATAACGTAAAATCGTGGTAAGACCTGCCGGGATTTAGTTGCAAATTTTTCAACATTTTATA  >  NC_000913/1151275‑1151565

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 17 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A