Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A3 F50 I2 R1	257	49.9	1554294	99.0%	1538751	150.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,417,189	A→G	A66A (GCT→GCC)	recE ←	Rac prophage; exonuclease VIII, 5' to 3' specific dsDNA exonuclease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,417,189	0	A	G	100.0%	190.0 / NA	53	A66A (GCT→GCC)	recE	Rac prophage; exonuclease VIII, 5' to 3' specific dsDNA exonuclease
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (27/26); total (27/26)

CCTCAATCTCAGTCATGTCTTCGCCGTTGACGTTGGTATTGCCTTGATAGTGAGCGTTGTCTGGTGCTGCTCCCGGTTTTAGTTCCCATGTCATGGAGTCTTTGCTGAGTTGATAGCGTTCACTCCAGGTAAAATCGATCTCACCTTCAGCGGGCAGGTCATTAACGACAGGAAAATTCGTGGCAACAGCTTTAAAATAGCTGCTCAGTTTTTTACCTGACTTAACGATCAGGTAGTCCAGAGTGGCACAGGTCGATTCAAAATCGTTGCTTGCCCACAGGACGACGTCA  >  NC_000913/1417041‑1417330

cctcAATCTCAGTCATGTCTTCGCCGTTGACGTTGGTATTGCCTTGATAGTGAGCGTTGTCTGGTGCTGCTCCCGGTTTTAGTTCCCATGTCATGGAGTCTTTGCTGAGTTGATAGCGTTCACTCCAGGTAAAATCGATCTCACCTTCGGc                                                                                                                                             <  1:58797/151‑1 (MQ=255)

      tctcAGTCATGTCTTCGCCGTTGACGTTGGTATTGCCTTGATAGTGAGCGTTGTCTGGTGCTGCTCCCGGTTTTAGTTCCCATGTCATGGAGTCTTTGCTGAGTTGATAGCGTTCACTCCAGGTAAAATCGATCTCACCTTCGGCGGGc                                                                                                                                         >  1:579622/1‑149 (MQ=255)

           gTCATGTCTTCGCCGTTGACGTTGTTATTGCCTTGATAGTGAGCGTTGTCTGGTGCTGCTCCCGGTTTTAGTTCCCATGTCATGGAGTCTTTGCTGAGTTGATAGCGTTCACTCCAGGTAAAATCGATCTCACCTTCGGCGGGCAGGTCAt                                                                                                                                  >  2:246849/1‑151 (MQ=255)

           gTCATGTCTTCGCCGTTGACGTTGGTATTGCCTTGATAGTGAGCGTTGTCTGGTGCTGCTCCCGGTTTTAGTTCCCATGTCATGGAGTCTTTGCTGAGTTGATAGCGTTCACTCCAGGTAAAATCGATCTCACCTTCGGCGGGCAGGTCAt                                                                                                                                  <  2:145293/151‑1 (MQ=255)

           gTCATGTCTTCGCCGTTGACGTTGGTATTGCCTTGATAGTGAGCGTTGTCTGGTGCTGCTCCCGGTTTTAGTTCCCATGTCATGGAGTCTTTGCTGAGTTGATAGCGTTCACTCCAGGTAAAATCGATCTCACCTTCGGCGGGCAGGTCAt                                                                                                                                  >  2:32844/1‑151 (MQ=255)

                gTCTTCGCCGTTGACGTTGGTATTGCCTTGATAGTGAGCGTTGTCTGGTGCTGCTCCCGGTTTTAGTTCCCATGTCATGGAGTCTTTGCTGAGTTGATAGCGTTCACTCCAGGTAAAATCGATCTCACCTTCGGCGGGCAGGTCATTAACg                                                                                                                             <  2:420059/151‑1 (MQ=255)

                        cGTTGACGTTGGTATTGCCTTGATAGTGAGCGTTGTCTGGTGCTGCTCCCGGTTTTAGTTCCCATGTCATGGAGTCTTTGCTGAGTTGATAGCGTTCACTCCAGGTAAAATCGATCTCACCTTCGGCGGGCAGGTCATTAACGACAGGaa                                                                                                                      <  2:474121/150‑1 (MQ=255)

                          ttGACGTTGGTATTGCCTTGATAGTGAGCGTTGTCTGGTGCTGCTCCCGGTTTTAGTTCCCATGTCATGGAGTCTTTGCTGAGTTGATAGCGTTCACTCCAGGTAAAATCGATCTCACCTTCGGCGGGCAGGTCATTAACGACAGGaaaa                                                                                                                    <  2:541893/150‑1 (MQ=255)

                                    tATTGCCTTGATAGTGAGCGTTGTCTGGTGCTGCTCCCGGTTTTAGTTCCCATGTCATGGAGTCTTTGCTGAGTTGATAGCGTTCACTCCAGGTAAAAGCGATCTCACCTTCGGCGGGCAGGTCATTAACGCCAGGAAAATTCGTGGcg                                                                                                           <  1:149021/149‑2 (MQ=255)

                                     aTTGCCTTGATAGTGAGCGTTGTCTGGTGCTGCTCCCGGTTTTAGTTCCCATGTCATGGAGTCTTTGCTGAGTTGATAGCGTTCACTCCAGGTAAAATCGATCTCACCTTCGGCGGGCAGGTCATTAACGACAGGAAAATTCGTGGCAAc                                                                                                         <  2:386294/150‑1 (MQ=255)

                                     aTTGCCTTGATAGTGAGCGTTGTCTGGTGCTGCTCCCGGTTTTAGTTCCCATGTCATGGAGTCTTTGCTGAGTTGATAGCGTTCACTCCAGGTAAAATCGATCTCACCTTCGGCGGGCAGGTCATTAACGACAGGAAAATTCGTGGCAAc                                                                                                         <  2:481039/150‑1 (MQ=255)

                                        gCCTTGATAGTGAGCGTTGTCTGGTGCTGCTCCCGGTTTTAGTTCCCATGTCATGGAGTCTTTGCTGAGTTGATAGCGTTCACTCCAGGTAAAATCGATCTCACCTTCGGCGGGCAGGTCATTAACGACAGGAAAATTCGTGGCAACAGc                                                                                                      <  2:366336/150‑1 (MQ=255)

                                        gCCTTGATAGTGAGCGTTGTCTGGTGCTGCTCCCGGTTTTAGTTCCCATGTCATGGAGTCTTTGCTGAGTTGATAGCGTTCACTCCAGGTAAAATCGATCTCACCTTCGGCGGGCAGGTCATTAACGACAGGAAAATTCGTGGCAACAGCt                                                                                                     <  1:271568/151‑1 (MQ=255)

                                         ccTTGATAGTGAGCGTTGTCTGGTGCTGCTCCCGGTTTTAGTTCCCATGTCATGGAGTCTTTGCTGAGTTGATAGCGTTCACTCCAGGTAAAATCGATCTCACCTTCGGCGGGCAGGTCATTAACGACAGGAAAATTCGTGGCAACAGCtt                                                                                                    <  1:454267/151‑1 (MQ=255)

                                           ttGATAGTGAGCGTTGTCTGGTGCTGCTCCCGGTTTTAGTTCCCATGTCATGGAGTCTTTGCTGAGTTGATAGCGTTCACTCCAGGTAAAATCGATCTCACCTTCGGCGGGCAGGTCATTAACGACAGGAAAATTCGTGGCAACAGCttt                                                                                                   >  2:714/1‑150 (MQ=255)

                                             gATAGTGAGCGTTGTCTGGTGCTGCTCCCGGTTTTAGTTCCCATGTCATGGAGTCTTTGCTGAGTTGATAGCGTTCACTCCAGGTAAAATCGATCTCACCTTCGGCGGGCAGGTCATTAACGACAGGAAAATTCGTGGCAACAGCTTTaaa                                                                                                <  1:182597/151‑1 (MQ=255)

                                                aGTGAGCGTTGTCTGGTGCTGCTCCCGGTTTTAGTTCCCATGTCATGGAGTCTTTGCTGAGTTGATAGCGTTCACTCCAGGTAAAATCGATCTCACCTTCGGCGGGCAGGTCATTAACGACAGGAAAATTCGTGGCAACAGCTTTAAAAt                                                                                              >  1:132939/1‑150 (MQ=255)

                                                      cGTTGTCTGGTGCTGCTCCCGGTTTTAGTTCCCATGTCATGGAGTCTTTGCTGAGTTGATAGCGTTCACTCCAGGTAAAATCGATCTCACCTTCGGCGGGCAGGTCATTAACGACAGGAAAATTCGTGGCAACAGCTTTAAAATAgctgct                                                                                       <  1:427681/151‑1 (MQ=255)

                                                                 gctgctCCCGGTTTTAGTTCCCATGTCATGGAGTCTTTGCTGAGTTGATAGCGTTCACTCCAGGTAAAATCGATCTCACCTTCGGCGGGCAGGTCATTAACGACAGGAAAATTCGTGGCAACAGCTTTAAAATAGCTGCTCAGTTTTTTAc                                                                            >  1:48471/1‑151 (MQ=255)

                                                                       cccGGTTTTAGTTCCCATGTCATGGAGTCTTTGCTGAGTTGATAGCGTTCACTCCAGGTAAAATCGATCTCACCTTCGGCGGGCAGGTCATTAACGACAGGAAAATTCGTGGCAACAGCTTTAAAATAGCTGCTCAGTTTTTTACCTGACt                                                                      <  1:539148/151‑1 (MQ=255)

                                                                        ccGGTTTTAGTTCCCATGTCATGGAGTCTTTGCTGAGTTGATAGCGTTCACTCCAGGTAAAATCGATCTCACCTTCGGCGGGCAGGTCATTAACGACAGGAAAATTCGTGGCAACAGCTTTAAAATAGCTGCTCAGTTTTTTACCTGACtt                                                                     <  2:753780/151‑1 (MQ=255)

                                                                         cGGTTTTAGTTCCCATGTCATGGAGTCTTTGCTGAGTTGATAGCGTTCACTCCAGGTAAAATCGATCTCACCTTCGGCGGGCAGGTCATTAACGACAGGAAAATTCGTGGCAACAGCTTTAAAATAGCTGCTCAGTTTTTTACCTGACTTa                                                                    <  2:307739/151‑1 (MQ=255)

                                                                              ttAGTTCCCATGTCATGGAGTCTTTGCTGAGTTGATAGCGTTCACTCCAGGTAAAATCGATCTCACCTTCGGCGGGCAGGTCATTAACGACAGGAAAATTCGTGGCAACAGCTTTAAAATAGCTGCTCAGTTTTTTACCTGACTTAACGa                                                                <  1:240309/150‑1 (MQ=255)

                                                                              ttAGTTCCCATGTCATGGAGTCTTTGCTGAGTTGATAGCGTTCACTCCAGGTAAAATCGATCTCACCTTCGGCGGGCAGGTCATTAACGACAGGAAAATTCGTGGCAACAGCTTTAAAATAGCTGCTCAGTTTTTTACCTGACTTAACGa                                                                <  2:445838/150‑1 (MQ=255)

                                                                              ttAGTTCCCATGTCATGGAGTCTTTGCTGAGTTGATAGCGTTCACTCCAGGTAAAATCGATCTCACCTTCGGCGGGCAGGTCATTAACGACAGGAAAATTCGTGGCAACAGCTTTAAAATAGCTGCTCAGTTTTTTACCTGACTTAACGAt                                                               >  1:421209/1‑151 (MQ=255)

                                                                                  ttCCCATGTCATGGAGTCTTTGCTGAGTTGATAGCGTTCACTCCAGGTAAAATCGATCTCACCTTCGGCGGGCAGGTCATTAACGACAGGAAAATTCGTGGCAACAGCTTTAAAATAGCTGCTCAGTTTTTTACCTGACTTAACGATCAgg                                                           >  1:184421/1‑151 (MQ=255)

                                                                                      cATGTCATGGAGTCTTTGCTGAGTTGATAGCGTTCACTCCAGGTAAAATCGATCTCACCTTCGGCGGGCAGGTCATTAACGACAGGAAAATTCGTGGCAACAGCTTTAAAATAGCTGCTCAGTTTTTTACCTGACTTAACGATCAGGTAGt                                                       >  1:396439/1‑151 (MQ=255)

                                                                                          tCATGGAGTCTTTGCTGAGTTGATAGCGTTCACTCCAGGTAAAATCGATCTCACCTTCGGCGGGCAGGTCATTAACGACAGGAAAATTCGTGGCAACAGCTTTAAAATAGCTGCTCAGTTTTTTACCTGACTTAACGATCAGGTAGTcc                                                     >  1:344660/1‑149 (MQ=255)

                                                                                          tCATGGAGTCTTTGCTGAGTTGATAGCGTTCACTCCAGGTAAAATCGATCTCACCTTCGGCGGGCAGGTCATTAACGACAGGAAAATTCGTGGCAACAGCTTTAAAATAGCTGCTCAGTTTTTTACCTGACTTAACGATCAGGTAGTcc                                                     >  2:521519/1‑149 (MQ=255)

                                                                                           cATGGAGTCTTTGCTGAGTTGATAGCGTTCACTCCAGGTAAAATCGATCTCACCTTCGGCGGGCAGGTCATTAACGACAGGAAAATTCGTGGCAACAGCTTTAAAATAGCTGCTCAGTTTTTTACCTGACTTAACGATCAGGTAGTCCaga                                                  <  1:400424/151‑1 (MQ=255)

                                                                                           cATGGAGTCTTTGCTGAGTTGATAGCGTTCACTCCAGGTAAAATCGACCTCACCTTCGGCGGGCAGGTCATTAACGACAGGAAAATTCGTGGCAACAGCTTTAAAATAGCTGCTCAGTTTTTTACCTGACTTAACGATCAGGTAGTcc                                                     >  2:566648/1‑148 (MQ=255)

                                                                                             tGGAGTCTTTGCTGAGTTGATAGCGTTCACTCCAGGTAAAATCGATCTCACCTTCGGCGGGCAGGTCATTAACGACAGGAAAATTCGTGGCAACAGCTTTAAAATAGCTGCTCAGTTTTTTACCTGACTTAACGATCAGGTAGTCCagag                                                 >  2:668473/1‑150 (MQ=255)

                                                                                                    tttGCTGAGTTGATAGCGTTCACTCCAGGTAAAATCGATCTCACCTTCGGCGGGCAGGTCATTAACGACAGGAAAATTCGTGGCAACAGCTTTAAAATAGCTGCTCAGTTTTTTACCTGACTTAACGATCAGGTAGTCCAGAGTGGcac                                           >  1:215493/1‑149 (MQ=255)

                                                                                                      tGCTGAGTTGATAGCGTTCACTCCAGGTAAAATCGATCTCACCTTCGGCGGGCAGGTCATTAACGACAGGAAAATTCGTGGCAACAGCTTTAAAATAGCTGCTCAGTTTTTTACCTGACTTAACGATCAGGTAGTCCAGAGTGGCACAGGt                                       >  2:703794/1‑151 (MQ=255)

                                                                                                       gCTGAGTTGATAGCGTTCACTCCAGGTAAAATCGATCTCACCTTCGGCGGGCAGGTCATTAACGACAGGAAAATTCGTGGCAACAGCTTTAAAATAGCTGCTCAGTTTTTTACCTGACTTAACGATCAGGTAGTCCAGAGTGGcacacg                                        <  1:644264/149‑3 (MQ=255)

                                                                                                         tGAGTTGATAGCGTTCACTCCAGGTAAAATCGATCTCACCTTCGGCGGGCAGGTCATTAACGACAGGAAAATTCGTGGCAACAGCTTTAAAATAGCTGCTCAGTTTTTTACCTGACTTAACGATCAGGTAGTCCAGAGTGGCACAGGTCg                                     >  2:742033/1‑150 (MQ=255)

                                                                                                             ttGATAGCGTTCACTCCAGGTAAAATCGATCTCACCTTCGGCGGGCAGGTCATTAACGACAGGAAAATTCGTGGCAACAGCTTTAAAATAGCTGCTCAGTTTTTTACCTGACTTAACGATCAGGTAGTCCAGAGTGGCACAGGTCGATTc                                 >  1:491075/1‑150 (MQ=255)

                                                                                                               gATAGCGTTCACTCCAGGTAAAATCGATCTCACCTTCGGCGGGCAGGTCATTAACGACAGGAAAATTCGTGGCAACAGCTTTAAAATAGCTGCTCAGTTTTTTACCTGACTTAACGATCAGGTAGTCCAGAGTGGCACAGGTCGATTCaa                               >  1:72004/1‑150 (MQ=255)

                                                                                                                aTAGCGTTCACTCCAGGTAAAATCGATCTCACCTTCGGCGGGCAGGTCATTAACGACAGGAAAATTCGTGGCAACAGCTTTAAAATAGCTGCTCAGTTTTTTACCTGACTTAACGATCAGGTAGTCCAGAGTGGCACAGGTCGATTCaaa                              >  2:234679/1‑150 (MQ=255)

                                                                                                                    cGTTCACTCCAGGTAAAATCGATCTCACCTTCGGCGGGCAGGTCATTAACGACAGGAAAATTCGTGGCAACAGCTTTAAAATAGCTGCTCAGTTTTTTACCTGACTTAACGATCAGGTAGTCCAGAGTGGCACAGGTCGATTCAAAATCGt                         >  2:226718/1‑151 (MQ=255)

                                                                                                                        cACTCCAGGTAAAATCGATCTCACCTTCGGCGGGCAGGTCATTAACGACAGGAAAATTCGTGGCAACAGCTTTAAAATAGCTGCTCAGTTTTTTACCTGACTTAACGATCAGGTAGTCCAGAGTGGCACAGGTCGATTCAAAATCGttgct                     >  2:683048/1‑151 (MQ=255)

                                                                                                                         aCTCCAGGTAAAATCGATCTCACCTTCGGCGGGCAGGTCATTAACGACAGGAAAATTCGTGGCAACAGCTTTAAAATAGCTGCTCAGTTTTTTACCTGACTTAACGATCAGGTAGTCCAGAGTGGCACAGGTCGATTCAAAATCGttgctt                    <  2:104835/151‑1 (MQ=255)

                                                                                                                          atCCAGGTAAAATCGATCTCACCTTCGGAGGGCAGGTCATTAACGACAGGAAAATTCGTGGCAACAGCTTTAAAATAGCTGCTCAGTTTTTTAACTGACTTAACGAAAAGGTAGTCCAGAGTGGCACAGGTCGATTCAAAATCGttgct                     >  1:207130/2‑149 (MQ=255)

                                                                                                                            ccAGGTAAAATCGATCTCACCTTCGGCGGGCAGGTCATTAACGACAGGAAAATTCGTGGCAACAGCTTTAAAATAGCTGCTCAGTTTTTTACCTGACTTAACGATCAGGTAGTCCAGAGTGGCACAGGTCGATTCAAAATCGttgcttgc                  <  1:662898/150‑1 (MQ=255)

                                                                                                                            ccAGGTAAAATCGATCTCACCTTCGGCGGGCAGGTCATTAACGACAGGAAAATTCGTGGCAACAGCTTTAAAATAGCTGCTCAGTTTTTTACCTGACTTAACGATCAGGTAGTCCAGAGTGGCACAGGTCGATTCAAAATCGttgcttg                   <  1:297562/149‑1 (MQ=255)

                                                                                                                              aGGTAAAATCGATCTCACCTTCGGCGGGCAGGTCATTAACGACAGGAAAATTCGTGGCAACAGCTTTAAAATAGCTGCTCAGTTTTTTACCTGACTTAACGATCAGGTAGTCCAGAGTGGCACAGGTCGATTCAAAATCGTTGCTTGccc                >  1:569690/1‑150 (MQ=255)

                                                                                                                              aGGTAAAATCGATCTCACCTTCGGCGGGCAGGTCATTAACGACAGGAAAATTCGTGGCAACAGCTTTAAAATAGCTGCTCAGTTTTTTACCTGACTTAACGATCAGGTAGTCCAGAGTGGCACAGGTCGATTCAAAATCGTTGCTTGccc                >  2:576774/1‑150 (MQ=255)

                                                                                                                              aGGTAAAATCGATCTCACCTTCGGCGGGCAGGTCATTAACGACAGGAAAATTCGTGGCAACAGCTTTAAAATAGCTGCTCAGTTTTTTACCTGACTTAACGATCAGGTAGTCCAGAGTGGCACAGGTCGATTCAAAATCGTTGCTTGCCca               <  1:63479/151‑1 (MQ=255)

                                                                                                                                  aaaaTCGATCTCACCTTCGGCGGGCAGGTCATTAACGACAGGAAAATTCGTGGCAACAGCTTTAAAATAGCTGCTCAGTTTTTTACCTGACTTAACGATCAGGTAGTCCAGAGTGGCACAGGTCGATTCAAAATCGTTGCTTGCCCACAgg           >  1:665572/1‑151 (MQ=255)

                                                                                                                                    aaTCGATCTCACCTTCGGCGGGCAGGTCATTAACGACAGGAAAATTCGTGGCAACAGCTTTAAAATAGCTGCTCAGTTTTTTACCTGACTTAACGATCAGGTAGTCCAGAGTGGCACAGGTCGATTCAAAATCGTTGCTTGCCCACAGgac         <  2:343505/151‑1 (MQ=255)

                                                                                                                                        gATCTCACCTTCGGCGGGCAGGTCATTAACGACAGGAAAATTCGTGGCAACAGCTTTAAAATAGCTGCTCAGTTTTTTACCTGACTTAACGATCAGGTAGTCCAGAGTGGCACAGGTCGATTCAAAATCGTTGCTTGCCCACAGGacgacg     >  1:19281/1‑151 (MQ=255)

                                                                                                                                         aTCTCACCTTCGGCGGGCCGGTCATTAACGACAGGAAACTTCGTGGCAACAGCTTTAAAATAGCTGCTCAGTTTTTTACCTGACTTAACGATCAGGTAGTCCAGAGTGGCACAGGTCGATTCAAAATCGTTGCTTGCCCACAGGACGACGt    >  1:396931/1‑151 (MQ=255)

                                                                                                                                             ctccTTCGGCGGGCAGGTCATTAACGACAGGAAAATTCGTGGCAACAGCTTTAAAATAGCTGCTCAGTTTTTTACCTGACTTAACGATCAGGTAGTCCAGAGTGGCACAGGTCGATTCAAAATCGTTGCTTGCCCACAGGACGACGTCa  <  2:461967/147‑1 (MQ=255)

CCTCAATCTCAGTCATGTCTTCGCCGTTGACGTTGGTATTGCCTTGATAGTGAGCGTTGTCTGGTGCTGCTCCCGGTTTTAGTTCCCATGTCATGGAGTCTTTGCTGAGTTGATAGCGTTCACTCCAGGTAAAATCGATCTCACCTTCAGCGGGCAGGTCATTAACGACAGGAAAATTCGTGGCAACAGCTTTAAAATAGCTGCTCAGTTTTTTACCTGACTTAACGATCAGGTAGTCCAGAGTGGCACAGGTCGATTCAAAATCGTTGCTTGCCCACAGGACGACGTCA  >  NC_000913/1417041‑1417330

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A