Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A3 F50 I2 R1	257	49.9	1554294	99.0%	1538751	150.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,602,723	G→A	R302R (CGG→CGA)	hyfB →	hydrogenase 4, membrane subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,602,723	0	G	A	100.0%	162.0 / NA	45	R302R (CGG→CGA)	hyfB	hydrogenase 4, membrane subunit
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (19/26); total (19/26)

GTATTTTCGGCATCCTGAAAGTAGCGATGGATCTGCTGGCGCAAACGGGTTTGCCTCTGTGGTGGGGCATTCTGGTGATGGCGATCGGCGCAATCTCCGCGCTCCTGGGCGTGCTATATGCGCTGGCGGAACAGGATATCAAACGGCTGCTGGCCTGGAGTACCGTCGAAAACGTCGGCATTATTTTGCTGGCAGTCGGTGTGGCGATGGTCGGTCTGTCACTGCACGACCCGCTGCTCACCGTGGTTGGACTGCTCGGCGCACTGTTTCATCTGCTCAACCATGCGCT  >  NC_000913/2602578‑2602866

gTATTTTCGGCATCCTGAAAGTAGCGATGGATCTGCTGGCGCAAACGGGTTTGCCTCTGTGGTGGGGCATTCTGGTGATGGCGATCGGCGCAATCTCCGCGCTCCTGGGCGTGCTATATGCGCTGGCGGAACAGGATATCAAACGactgct                                                                                                                                            >  2:223738/1‑151 (MQ=255)

        ggCATCCTGAAAGTAGCGATGGATCTGCTGGCGCAAACGGGTTTGCCTCTGTGGTGGGGCATTCTGGTGATGGCGATCGGCGCAATCTCCGCGCTCCTGGGCGTGCTATATGCGCTGGCGGAACAGGATATCAAACGACTGCTGGCCTGGa                                                                                                                                    <  1:470255/151‑1 (MQ=255)

         gCATCCTGAAAGTAGCGATGGATCTGCTGGCGCAAACGGGTTTGCCTCTGTGGTGGGGCATTCTGGTGATGGCGATCGGCGCAATCTCCGCGCTCCTGGGCGTGCTATATGCGCTGGCGGAACAGGATATCAAACGACTGCTGGCCTGGAg                                                                                                                                   <  1:168380/151‑1 (MQ=255)

           aTCCTGAAAGTAGCGATGGATCTGCTGGCGCAAACGGGTTTGCCTCTGTGGTGGGGCATTCTGGTGATGGCGATCGGCGCAATCTCCGCGCTCCTGGGCGTGCTATATGCGCTGGCGGAACAGGATATCAAACGACTGCTGGCCTGGAGTa                                                                                                                                 <  2:584324/151‑1 (MQ=255)

                gAAAGTAGCGATGGATCTGCTGGCGCAAACGGGTTTGCCTCTGTGGTGGGGCATTCTGGTGATGGCGGTCGGCGCAATCTCCGCGCTCCTGGGCGTGCTATATGCGCTGGCGGAACAGGATATCAAACGACTGCTGGCCTGGAGTGCCGTc                                                                                                                            <  1:63300/151‑1 (MQ=255)

                gAAAGTAGCGATGGATCTGCTGGCGCAAACGGGTTTGCCTCTGTGGTGGGGCATTCTGGTGATGGCGATCGGCGCAATCTCCGCGCTCCTGGGCGTGCTATATGCGCTGGCGGAACAGGATATCAAACGACTGCTGGCCTGGAGTACCg                                                                                                                              <  2:421478/149‑1 (MQ=255)

                gAAAGTAGCGATGGATCTGCTGGCGCAAACGGGTTTGCCTCTGTGGTGGGGCATTCTGGTGATGGCGATCGGCGCAATCTCCGCGCTCCTGGGCGTGCTATATGCGCTGGCGGAACAGGATATCAAACGACTGCTGGCCTGGAGTACCGTc                                                                                                                            >  1:29600/1‑151 (MQ=255)

                           tGGATCTGCTGGCGCAAACGGGTTTGCCTCTGTGGTGGGGCATTCTGGTGATGGCGATCGGCGCAATCTCCGCGCTCCTGGGCGTGCTATATGCGCTGGCGGAACAGGATATCAAACGACTGCTGGCCTGGAGTACCGTCGAAAACGTCg                                                                                                                  >  1:727613/1‑150 (MQ=255)

                            ggctCTGCTGGCGCAAACGGGTTTGCCTCTGTGGTGGGGCATTCTGGTGGTGGCGATCGGCGCAATCTCCGCGCTCCTGGGCGTGCTATATGCGCTGGCGGAACAGGATATCAAACGACTGCGGGCCTGGAGGACCGTCGAAAACg                                                                                                                     >  1:68219/4‑146 (MQ=255)

                             gATCTGCTGGCGCAAACGGGTTTGCCTCTGTGGTGGGGCATTCTGGTGATGGCGATCGGCGCAATCTCCGCGCTCCTGGGCGTGCTATATGCGCTGGCGGAACAGGATATCAAACGACTGCTGGCCTGGAGTACCGTCGAAAACGTCGGc                                                                                                                >  1:168068/1‑150 (MQ=255)

                                ctgctgGCGCAAACGGGTTTGCCTCTGTGGTGGGGCATTCTGGTGATGGCGATCGGCGCAATCTCCGCGCTCCTGGGCGTGCTATATGCGCTGGCGGAACAGGATATCAAACGACTGCTGGCCTGGAGTACCGTCGAAAACGTCGGCatt                                                                                                             >  2:87946/1‑150 (MQ=255)

                                  gctgGCGCAAACGGGTTTGCCTCTGTGGTGGGGCATTCTGGTGATGGCGATCGGCGCAATCTCCGCGCTCCTGGGCGTGCTATATGCGCTGGCGGAACAGGATATCAAACGACTGCTGGCCTGGAGTACCGTCGAAAACGTCGGCattatt                                                                                                          <  1:76931/151‑1 (MQ=255)

                                  gctgGCGCAAACGGGTTTGCCTCTGTGGTGGGGCATTCTGGTGATGGCGATCGGCGCAATCTCCGCGCTCCTGGGCGTGCTATATGCGCTGGCGGAACAGGATATCAAACGACTGCTGGCCTGGAGTACCGTCGAAAACGTCGGCattat                                                                                                           <  2:615454/150‑1 (MQ=255)

                                  gctgGCGCAAACGGGTTTGCCTCTGTGGTGGGGCATTCTGGTGATGGCGATCGGCGCAATCTCCGCGCTCCTGGGCGTGCTATATGCGCTGGCGGAACAGGATATCAAACGACTGCTGGCCTGGAGTACCGTCGAAAACGTCGGCatt                                                                                                             <  2:303543/148‑1 (MQ=255)

                                      gcgcAAACGGGTTTGCCTCTGTGGTGGGGCATTCTGGTGATGGCGATCGGCGCAATCTCCGCGCTCCTGGGCGTGCTATATGCGCTGGCGGAACAGGATATCAAACGACTGCTGGCCTGGAGTACCGTCGAAAACGTCGGCATTATTTTGc                                                                                                      >  1:579654/1‑151 (MQ=255)

                                         cAAACGGGTTTGCCTCTGTGGTGGGGCATTCTGGTGATGGCGATCGGCGCAATCTCCGCGCTCCTGGGCGTGCTATATGCGCTGGCGGAACAGGATATCAAACGACTGCTGGCCTGGAGTACCGTCGAAAACGTCGGCATTATTTTGCTgg                                                                                                   >  1:435930/1‑151 (MQ=255)

                                           aaCGGGTTTGCCTCTGTGGTGGGGCATTCTGGTGATGGCGATCGGCGCAATCTCCGCGCTCCTGGGCGTGCTATATGCGCTGGCGGAACAGGATATCAAACGACTGCTGGCCTGGAGTACCGTCGAAAACGTCGGCATTATTTTGCTGGCa                                                                                                 <  1:253378/151‑1 (MQ=255)

                                              gggTTTGCCTCTGTGGTGGGGCATTCTGGTGATGGCGATCGGCGCAATCTCCGCGCTCCTGGGCGTGCTATATGCGCTGGCGGAACAGGATATCAAACGACTGCTGGCCTGGAGTACCGTCGAAAACGTCGGCATTATTTTGCTGGCAGTc                                                                                              >  1:449649/1‑151 (MQ=255)

                                                  ttGCCTCTGTGGTGGGGCATTCTGGTGATGGCGATCGGCGCAATCTCCGCGCTCCTGGGCGTGCTATATGCGCTGGCGGAACAGGATATCAAACGACTGCTGGCCTGGAGTACCGTCGAAAACGTCGGCATTATTTTGCTGGCAGTCGgtg                                                                                          >  1:285467/1‑151 (MQ=255)

                                                    gCCTCTGTGGTGGGGCATTCTGGTGATGGCGATCGGCGCAATCTCCGCGCTCCTGGGCGTGCTATATGCGCTGGCGGAACAGGATATCAAACGACTGCTGGCCTGGAGTACCGTCGAAAACGTCGGCATTATTTTGCTGGCAGTCGgtgt                                                                                         <  2:234223/150‑1 (MQ=255)

                                                            ggtggGGCATTCTGGTGATGGCGATCGGCGCAATCTCCGCGCTCCTGGGCGTGCTATATGCGCTGGCGGAACAGGATATCAAACGACTGCTGGCCTGGAGTACCGTCGAAAACGTCGGCATTATTTTGCTGGCAGTCGGTGTGGCGATgg                                                                                 <  2:459603/150‑1 (MQ=255)

                                                               ggggCATTCTGGTGATGGCGATCGGCGCAATCTCCGCGCTCCTGGGCGTGCTATATGCGCTGGCGGAACAGGATATCAAACGACTGCTGGCCTGGAGTACCGTCGAAAACGTCGGCATTATTTTGCTGGCAGTCGGTGTGGCGATggtcg                                                                              <  2:493430/150‑1 (MQ=255)

                                                                gggCATTCTGGTGATGGCGATCGGCGCAATCTCCGCGCTCCTGGGCGTGCTATATGCGCTGGCGGAACAGGATATCAAACGACTGCTGGCCTGGAGTACCGTCGAAAACGTCGGCATTATTTTGCTGGCAGGCGGTGTGGAGATggt                                                                                <  1:448852/147‑1 (MQ=255)

                                                                 ggCATTCTGGTGATGGCGATCGGCGCAATCTCCGCGCTCCTGGGCGTGCTATATGCGCTGGCGGAACAGGATATCAAACGACTGCTGGCCTGGAGTACCGTCGAAAACGTCGGCATTATTTTGCTGGCAGTCGGTGTGGCGATggtcggtc                                                                           <  2:291821/151‑1 (MQ=255)

                                                                        tGGTGATGGCGATCGGCGCAATCTCCGCGCTCCTGGGCGTGCTATATGCGCTGGCGGAACAGGATATCAAACGACTGCTGGCCTGGAGTACCGTCGAAAACGTCGGCATTATTTTGCTGGCAGTCGGTGTGGCGATGGTCGGTCTGTCACt                                                                    >  2:230674/1‑151 (MQ=255)

                                                                            gATGGAGATCGTCGCAATCTCCGCGCTCCTGGGCGAGCTATATGAGCTGGCGGGACAGGATATCAAACGACTGCTGGCCTGGTGTACCGTCGAAAACGTCGGCATTATTTTGCTGGCAGTCGGTGTGGCGATGGTAGGTCTGTCACTGctc                                                                >  1:522540/1‑149 (MQ=255)

                                                                                 cGATCGGCGCAATCTCCGCGCTCCTGGGCGTGCTATATGCGCTGGCGGAACAGGATATCAAACGACTGCTGGCCTGGAGTACCGTCGAAAACGTCGGCATTATTTTGCTGGCAGTCGGTGTGGCGATGGTCGGTCTGTCACTGCACGAccc                                                           >  1:118754/1‑151 (MQ=255)

                                                                                 cGATCGGCGCAATCTCCGCGCTCCTGGGCGTGCTATATGCGCTGGCGGAACAGGATATCAAACGACTGCTGGCCTGGAGTACCGTCGAAAACGTCGGCATTATTTTGCTGGCAGTCGGTGTGGCGATGGTCGGTCTGTCACTGCACGAccc                                                           >  2:411886/1‑151 (MQ=255)

                                                                                  gATCGGCGCAATCTCCGCGCTCCTGGGCGTGCTATATGCGCTGGCGGAACAGGATATCAAACGACTGCTGGCCTGGAGTACCGTCGAAAACGTCGGCATTATTTTGCTGGCAGTCGGTGTGGCGATGGTCGGTCTGTCACTGCACGACCCg                                                          <  2:266311/151‑1 (MQ=255)

                                                                                  gATCGGCGCAATCTCCGCGCTCCTGGGCGTGCTATATGCGCTGGCGGAACAGGATATCAAACGACTGCTGGCCTGGAGTACCGTCGAAAACGTCGGCATTATTTTGCTGGCAGTCGGTGTGGCGATGGTCGGTCTGTCACTGCACGACCCg                                                          >  2:750431/1‑151 (MQ=255)

                                                                                  gATCGGCGCAATCTCCGCGCTCCTGGGCGTGCTATATGCGCTGGCGGAACAGGATATCAAACGACTGCTGGCCTGGAGTACCGTCGAAAACGTCGGCATTATTTTGCTGGCAGTCGGTGTGGCGATGGTCGGTCTGTCACTGCACGACCCg                                                          >  1:307993/1‑151 (MQ=255)

                                                                                         gcAATCTCCGCGCTCCTGGGCGTGCTATATGCGCTGGCGGAACAGGATATCAAACGACTGCTGGCCTGGAGTACCGTCGAAAACGTCGGCATTATTTTGCTGGCAGTCGGTGTGGCGATGGTCGGTCTGTCACTGCACGACCCGCTGCTCa                                                   <  1:718143/151‑1 (MQ=255)

                                                                                         gcAATCTCCGCGCTCCTGGGCGTGCTATATGCGCTGGCGGAACAGGATATCAAACGACTGCTGGCCTGGAGTACCGTCGAAAACGTCGGCATTATTTTGCTGGCAGTCGGTGTGGCGATGGTCGGTCTGTCACTGCACGACCCGCTGCTCa                                                   <  2:435981/151‑1 (MQ=255)

                                                                                                       ccTGGGCGCGCTATATGCGCTGGCGGAACAGGATATCAAACGACTGCTGGCCTGGAGTACCGTCGAAAACGTCGGCATTATTTTGCTGGCAGTCGGTGTGGCGATGGTCGGTCTGTCACTGCACGACCCGCTGCTCACCGTGGTTGGACTg                                     >  2:230276/1‑151 (MQ=255)

                                                                                                           ggCGTGCTATATGCGCTGGCGGGACAGGATATCAAACGACTGCTGGCCTGTAGTACCGTCGAAAACGTCGGCATTATTTTGCTGGCAGTCGGTGTGGCGATGGTCGGTCTGTCACTGCACGACCCGCTGCTCACCGTGGTTGGACTGCt                                   <  2:617997/149‑1 (MQ=255)

                                                                                                            gCGTGCTATATGCGCTGGCGGAACAGGATATCAAACGACTGCTGGCCTGGAGTACCGTCGAAAACGTCGGCATTATTTTGCTGGCAGTCGGTGTGGCGATGGTCGGTCTGTCACTGCACGACCCGCTGCTCACCGTGGTTGGACTGCTCgg                                <  2:278875/151‑1 (MQ=255)

                                                                                                             cGTGCTATATGCGCTGGCGGAACAGGATATCAAACGACTGCTGGCCTGGAGTACCGTCGAAAACGTCGGCATTATTTTGCTGGCAGTCGGTGTGGCGATGGTCGGTCTGTCACTGCACGACCCGCTGCTCACCGTGGTTGGACTGCTCGgc                               >  2:427324/1‑151 (MQ=255)

                                                                                                             cGTGCTATATGCGCTGGCGGAACAGGATATCAAACGACTGCTGGCCTGGAGTACCGTCGAAAACGTCGGCATTATTTTGCTGGCAGTCGGTGTGGCGATGGTCGGTCTGTCACTGCACGACCCGCTGCTCACCGTGGTTGGACTGCTCGgc                               <  1:566412/151‑1 (MQ=255)

                                                                                                                  tataTGCGCTGGCGGAACAGGATATCAAACGACTGCTGGCCTGGAGTACCGTCGAAAACGTCGGCATTATTTTGCTGGCAGTCGGTGTGGCGATGGTCGGTCTGTCACTGCACGACCCGCTGCTCACCGTGGTTGGACTGCTCGGCGCACt                          >  2:489890/1‑151 (MQ=255)

                                                                                                                  tataTGCGCTGGCGGAACAGGATATCAAACGACTGCTGGCCTGGAGTACCGTCGAAAACGTCGGCATTATTTTGCTGGCAGTCGGTGTGGCGATGGTCGGTCTGTCACTGCACGACCCGCTGCTCACCGTGGTTGGACTGCTCGGCGCACt                          >  2:96519/1‑151 (MQ=255)

                                                                                                                   atatGCGCTGGCGGAACAGGATATCAAACGACTGCTGGCCTGGAGTACCGTCGAAAACGTCGGCATTATTTTGCTGGCAGTCGGTGTGGCGATGGTCGGTCTGTCACTGCACGACCCGCTGCTCACCGTGGTTGGACTGCTCGGCGCACt                          <  2:614107/150‑1 (MQ=255)

                                                                                                                   atatGCGCTGGCGGAACAGGATATCAAACGACTGCTGGCCTGGAGTACCGTCGAAAACGTCGGCATTATTTTGCTGGCAGTCGGTGTGGCGATGGTCGGTCTGTCACTGCACGACCCGCTGCTCACCGTGGTTGGACTGCTCGGCGCACTg                         <  1:75022/151‑1 (MQ=255)

                                                                                                                     atGCGCTGGCGGAACAGGATATCAAACGACTGCTGGCCTGGAGTACCGTCGAAAACGTCGGCATTATTTTGCTGGCAGTCGGTGTGGCGATGGTCGGTCTGTCACTGCACGACCCGCTGCTCACCGTGGTTGGACTGCTCGGCGCACTGt                        <  1:740721/150‑1 (MQ=255)

                                                                                                                                      gATATCAAACGACTGCTGGCCTGGAGTACCGTCGAAAACGTCGGCATTATTTTGCTGGCAGTCGGTGTGGCGATGGTCGGTCTGTCACTGCACGACCCGCTGCTCACCGTGGTTGGACTGCTCGGCGCACTGTTTCATCTGCTCAACCATg      <  2:100523/151‑1 (MQ=255)

                                                                                                                                           cAAACGACTGCTGGCCTGGAGTACCGTCGAAAACGTCGGCATTATTTTGCTGGCAGTCGGTGTGGCGATGGTCGGTCTGTCACTGCTCGACCCGCTGCTCACCGTGGTTGGACTGCTCGGCGCACTGTTTCATCTGCTCAACCATGCGCt  <  2:412715/150‑1 (MQ=255)

                                                                                                                                           cAAACGACTGCTGGCCTGGAGTACCGTCGAAAACGTCGGCATTATTTTGCTGGCAGTCGGTGTGGCGATGGTCGGTCTGTCACTGCACGACCCGCTGCTCACCGTGGTTGGACTGCTCGGCGCACTGTTTCATCTGCTCAACCATGCGCt  <  2:183399/150‑1 (MQ=255)

GTATTTTCGGCATCCTGAAAGTAGCGATGGATCTGCTGGCGCAAACGGGTTTGCCTCTGTGGTGGGGCATTCTGGTGATGGCGATCGGCGCAATCTCCGCGCTCCTGGGCGTGCTATATGCGCTGGCGGAACAGGATATCAAACGGCTGCTGGCCTGGAGTACCGTCGAAAACGTCGGCATTATTTTGCTGGCAGTCGGTGTGGCGATGGTCGGTCTGTCACTGCACGACCCGCTGCTCACCGTGGTTGGACTGCTCGGCGCACTGTTTCATCTGCTCAACCATGCGCT  >  NC_000913/2602578‑2602866

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A