Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A3 F50 I2 R1	257	49.9	1554294	99.0%	1538751	150.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,862,827	C→T	R165C (CGC→TGC)	ygbK →	FliA‑regulated DUF1537 family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,862,827	0	C	T	100.0%	140.5 / NA	39	R165C (CGC→TGC)	ygbK	FliA‑regulated DUF1537 family protein
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (21/18); total (21/18)

CCTGCCGGTCAACGGACGTACGGTTTATCAGGGGTATTTGTTCGTAATGAATCAACTGCTGGCCGAATCCGGGATGCGCCATCACCCGGTAAATCCCATGACCGACAGCTATCTTCCCCGTCTGGTTGAAGCGCAATCCACAGGGCGCTGCGGCGTCGTTTCGGCACATGTTTTCGAACAAGGTGTGGATGCCGTTCGTCAAGAGCTGGCTCGCTTACAGCAAGAGGGCTACCGCTACGCGGTGCTTGATGCGCTGACCGAACACCATCTGGAAATTCAGGGAGAAGCCTTG  >  NC_000913/2862682‑2862973

ccTGCCGGTCAACGGACGTACGGTTTATCAGGGGTATTTGTTCGTAATGAATCAACTGCTGGCCGAATCCGGGATGCGCCATCACCCGGTAAATCCCATGACCGACAGCCATCTTCCCCGTCTGGTTGAAGCGCAATCCACAGGGtgctgc                                                                                                                                               <  2:25577/151‑1 (MQ=255)

      ggTCAACGGACGTACGGTTTATCAGGGGTATTTGTTCGTAATGAATCAACTGCTGGCCGAATCCGGGATGCGCCATCACCCGGTAAATCCCATGACCGACAGCCATCTTCCCCGTCTGGTTGAAGCGCAATCCACAGGGTGCTGCGGcgtc                                                                                                                                         >  2:392789/1‑151 (MQ=255)

          aaCGGACGTACGGTTTATCAGGGGTATTTGTTCGTAATGAATCAACTGCTGGCCGAATCCGGGATGCGCCATCACCCGGTAAATCCCATGACCGACAGCCATCTTCCCCGTCTGGTTGAAGCGCAATCCACAGGGTGCTGCGGCGTCGttt                                                                                                                                     >  1:585627/1‑151 (MQ=255)

                      gTTTATCAGGGGTATTTGTTCGTAATGAATCAACTGCTGGCCGAATCCGGGATGCGCCATCACCCGGTAAATCCCATGACCGACAGCCATCTTCCCCGTCTGGTTGAAGCGCAATCCACAGGGTGCTGCGGCGTCGTTTCGGCACATGttt                                                                                                                         <  2:452465/151‑1 (MQ=255)

                           tCAGGGGTATTTGTTCGTAATGAATCAACTGCTGGCCGAATCCGGGATGCGCCATCACCCGGTAAATCCCATGACCGACAGCCATCTTCCCCGTCTGGTTGAAGCGCAATCCACAGGGTGCTGCGGCGTCGTTTCGGCACATGTTTTCGa                                                                                                                     >  1:168851/1‑150 (MQ=255)

                            cAGGGGTATTTGTTCGTAATGAATCAACTGCTGGCCGAATCCGGGATGCGCCATCACCCGGTAAATCCCATGACCGACAGCCATCTTCCCCGTCTGGTTGAAGCGCAATCCACAGGGTGCTGCGGCGTCGTTTCGGCACATGTTTTCGAAc                                                                                                                   >  1:277803/1‑151 (MQ=255)

                              ggggTATTTGTTCGTAATGAATCAACTGCTGGCCGAATCCGGGATGCGCCATCACCCGGTAAATCCCATGACCGACAGCCATCTTCCCCGTCTGGTTGAAGCGCAATCCACAGGGTGCTGCGGCGTCGTTTCGGCACATGTTTTCGAACa                                                                                                                  >  2:740500/1‑150 (MQ=255)

                                 gTATTTGTTCGTAATGAATCAACTGCTGGCCGAATCCGGGATGCGCCATCACCCGGTAAATCCCATGACCGACAGCCATCTTCCCCGTCTGGTTGAAGCGCAATCCACAGGGTGCTGCGGCGTCGTTTCGGCACATGTTTTCGAACAAGgt                                                                                                              <  2:78019/151‑1 (MQ=255)

                                  tATTTGTTCGTAATGAATCAACTGCTGGCCGAATCCGGGATGCGCCATCACCCGGTAAATCCCATGACCGACAGCCATCTTCCCCGTCTGGTTGAAGCGCAATCCACAGGGTGCTGCGGCGTCGTTTCGGCACATGTTTTCGAACAAGgtg                                                                                                             >  2:530585/1‑151 (MQ=255)

                                      tGTTCGTAATGAATCAACTGCTGGCCGAATCCGGGATGCGCCATCACCCGGTAAATCCCATGACCGACAGCCATCTTCCCCGTCTGGTTGAAGCGCAATCCACAGGGTGCTGCGGCGTCGTTTCGGCACATGTTTTCGAACAAGGTGTgg                                                                                                          >  1:205667/1‑150 (MQ=255)

                                         tCGTAATGAATCAACTGCTGGCCGAATCCGGGATGCGCCATCACCCGGTAAATCCCATGACCGACAGCCATCTTCCCCGTCTGGTTGAAGCGCAATCCACAGGGTGCTGCGGCGTCGTTTCGGCACATGTTTTCGAACAAGGTGTGGATGc                                                                                                      >  1:457853/1‑151 (MQ=255)

                                         tCGTAATGAATCAACTGCTGGCCGAATCCGGGATGCGCCATCACCCGGTAAATCCCATGACCGACAGCCATCTTCCCCGTCTGGTTGAAGCGCAATCCACAGGGTGCTGCGGCGTCGTTTCGGCACATGTTTTCGAACAAGGTGTGGATGc                                                                                                      >  2:166928/1‑151 (MQ=255)

                                           gTAATGAATCAACTGCTGGCCGAATCCGGGATGCGCCATCACCCGGTAAATCCCATGACCGACAGCCATCTTCCCCGTCTGGTTGAAGCGCAATCCACAGGGTGCTGCGGCGTCGTTTCGGCACATGTTTTCGAACAAGGTGTGGATGCCg                                                                                                    >  2:314345/1‑151 (MQ=255)

                                            tAATGAATCAACTGCTGGCCGAATCCGGGATGCGCCATCACCCGGTAAATCCCATGACCGACAGCCATCTTCCCCGTCTGGTTGAAGCGCAATCCACAGGGTGCTGCGGCGTCGTTTCGGCACATGTTTTCGAACAAGGTGTGGATGCCGt                                                                                                   >  2:261037/1‑151 (MQ=255)

                                                gAATCAACTGCTGGCCGAATCCGGGATGCGCCATCACCCGGTAAATCCCATGACCGACAGCCATCTTCCCCGTCTGGTTGAAGCGCAATCCACAGGGTGCTGCGGCGTCGTTTCGGCACATGTTTTCGAACAAGGTGTGGACGCCGTTCGt                                                                                               >  2:688644/1‑151 (MQ=255)

                                                 aaTCAACTGCTGGCCGAATCCGGGATGCGCCATCACCCGGTAAATCCCATGACCGACAGCCATCTTCCCCGTCTGGTTGAAGCGCAATCCACAGGGTGCTGCGGCGTCGTTTCGGCACATGTTTTCGAACAAGGTGTGGATGCCGTTCGTc                                                                                              <  1:87262/151‑1 (MQ=255)

                                                     aaCTGCTGGCCGAATCCGGGATGCGCCATCACCCGGTAAATCCCATGACCGACAGCCATCTTCCCCGTCTGGTTGAAGCGCAATCCACAGGGTGCTGCGGCGTCGTTTCGGCACATGTTTTCGAACAAGGTGTGGATGCCGTTCGTCa                                                                                             >  1:672692/1‑148 (MQ=255)

                                                     aaCTGCTGGCCGAATCCGGGATGCGCCATCACCCGGTAAATCCCATGACCGACAGCCATCTTCCCCGTCTGGTTGAAGCGCAATCCACAGGGTGCTGCGGCGTCGTTTCGGCACATGTTTTCGAACAAGGTGTGGATGCCGTTCGTCa                                                                                             >  1:27114/1‑148 (MQ=255)

                                                              ccGAATCCGGGATGCGCCATCACCCGGTAAATCCCATGACCGACAGCCATCTTCCCCGTCTGGTTGAAGCGCAATCCACAGGGTGCTGCGGCGTCGTTTCGGCACATGTTTTCGAACAAGGTGTGGATGCCGTTCGTCAAGAGCTGGCTCg                                                                                 <  2:310885/151‑1 (MQ=255)

                                                                    ccGGGATGCGCCATCACCCGGTAAATCCCATGACCGACAGCCATCTTCCCCGTCTGGTTGAAGCGCAATCCACAGGGTGCTGCGGCGTCGTTTCGGCACATGTTTTCGAACAAGGTGTGGATGCCGTTCGTCAAGAGCTGGCTCGCTTAc                                                                            <  1:392740/150‑1 (MQ=255)

                                                                     cGGGATGCGCCATCCCCCGGTAAATCCCATGACCGACAGCCATCTTCCCCGTCTGGTTGAAGCGCAATCCACAGGGTGCTGCGGCGTCGTTTCGGCACATGTTTTCGAACAAGGTGTGGATGCCGTTCGTCAAGAGCTGGCTCGCTTACa                                                                           <  1:83606/150‑1 (MQ=255)

                                                                      gggATGCGCCATCACCTGGTAAATCCCATGACCGACAGCCATCTTCCACGTCTGGTTGAAGCGCAATCCACAGGCTGCTGCGGCGTCGTTTCGGCACATGTTTTCGAACAAGGTGTGGATGCCGTTCGTCAAGAGCTGGCTCGCTTACa                                                                           >  2:169676/1‑149 (MQ=255)

                                                                       ggATGCGCCATCACCCGGTAAATCCCATGACCGACAGCCATCTTCCCCGTCTGGTTGAAGCGCAATCCACAGGGTGCTGCGGCGTCGTTTCGGCACATGTTTTCGAACAAGGTGTGGATGCCGTTCGTCAAGAGCTGGCTCGCTTACAGCa                                                                        <  2:700742/151‑1 (MQ=255)

                                                                       ggATGCGCCATCACCCGGTAAATCCCATGACCGACAGCCATCTTCCCCGTCTGGTTGAAGCGCAATCCACAGGGTGCTGCGGCGTCGTTTCGGCACATGTTTTCGAACAAGGTGTGGATGCCGTTCGTCAAGAGCTGGCTCGCTTACAGCa                                                                        <  2:468968/151‑1 (MQ=255)

                                                                       ggATGCGCCATCACCCGGTAAATCCCAGGACCGACAGCCATCTTCCCCGTCGGGTTGAAGCGTAATCCACAGGGTGCTGCGGCGTCGTTTCGGCACATGTTTTCGAACAAGGTTTGGATGCCGGTCGTCAAAAGCTGGCTCGCTTACAGcg                                                                        <  1:609872/151‑2 (MQ=255)

                                                                                aTCACCCGGTAAATCCCATGACCGACAGCCATCTTCCCCGTCTGGTTGAAGCGCAATCCACAGGGTGCTGCGGCGTCGTTTCGGCACATGTTTTCGAACAAGGTGTGGATGCCGTTCGTCAAGAGCTGGCTCGCTTACAGCAAGAGGGCt                                                                >  2:653610/1‑150 (MQ=255)

                                                                                  cACCCGGTAAATCCCATGACCGACAGCCATCTTCCCCGTCTGGTTGAAGCGCAATCCACAGGGTGCTGCGGCGTCGTTTCGGCACATGTTTTCGAACAAGGTGTGGATGCCGTTCGTCAAGAGCTGGCTCGCTTACAGCAAGAGGGCTAcc                                                             <  1:740426/151‑1 (MQ=255)

                                                                                         tAAATCCCATGACCGACAGCCATCTTCCCCGTCTGGTTGAAGCGCAATCCACAGGGTGCTGCGGCGTCGTTTCGGCACATGTTTTCGAACAAGGTGTGGATGCCGTTCGTCAAGAGCTGGCTCGCTTACAGCAAGAGGGCTACCGCTAcg                                                       >  2:469619/1‑150 (MQ=255)

                                                                                                   gACCGACAGCCATCTTCCCCGTCTGGTTGAAGCGCAATCCACAGGGTGCTGCGGCGTCGTTTCGGCACATGTTTTCGAACAAGGTGTGGATGCCGTTCGTCAAGAGCTGGCTCGCTTACAGCAAGAGGGCTACCGCTACGCGGTGCTTGAt                                            <  2:722924/151‑1 (MQ=255)

                                                                                                   gACCGACAGCCATCTTCCCCGTCTGGTTGAAGCGCAATCCACAGGGTGCTGCGGCGTCGTTTCGGCACATGTTTTCGAACAAGGTGTGGATGCCGTTCGTCAAGAGCTGGCTCGCTTACAGCAAGAGGGCTACCGCTACGCGGTGCTTGAt                                            >  1:558042/1‑151 (MQ=255)

                                                                                                        aCAGCCATCTTCCCCGTCTGGTTGAAGCGCAATCCACAGGGTGCTGCGGCGTCGTTTCGGCACATGTTTTCGAACAAGGTGTGGATGCCGTTCGTCAAGAGCTGGCTCGCTTACAGCAAGAGGGCTACCGCTACGCGGTGCTTGATGCGCt                                       <  2:681039/151‑1 (MQ=255)

                                                                                                            ccaTCTTCCCCGTCTGGTTGAAGCGCAATCCACAGGGTGCTGCGGCGTCGTTTCGGCACATGTTTTCGAACAAGGTGTGGATGCCGTTCGTCAAGAGCTGGCTCGCTTACAGCAAGAGGGCTACCGCTACGCGGTGCTTGATGCGCTGAcc                                   <  1:761034/149‑1 (MQ=255)

                                                                                                                          tGGTTGAAGCGCAATCCACAGGGTGCTGCGGCGTCGTTTCGGCACATGTTTTCGAACAAGGTGTGGATGCCGTTCGTCAAGAGCTGGCTCGCTTACAGCAAGAGGGCTACCGCTACGCGGTGCTTGATGCGCTGACCGAACACCATCTgg                      >  2:300023/1‑150 (MQ=255)

                                                                                                                           ggTTGAAGCGCAATCCACAGGGTGCTGCGGCGTCGTTTCGGCACATGTTTTCGAACAAGGTGTGGATGCCGTTCGTCAAGAGCTGGCTCGCTTACAGCAAGAGGGCTACCGCTACGCGGTGCTTGATGCGCTGACCGAACACCATCTgg                      <  1:369667/149‑1 (MQ=255)

                                                                                                                           ggTTGAAGCGCAATCCACAGGGTGCTGCGGCGTCGTTTCGGCACATGTTTTCGAACAAGGTGTGGATGCCGTTCGTCAAGAGCTGGCTCGCTTACAGCAAGAGGGCTACCGCTACGCGGTGCTTGATGCGCTGACCGAACACCATCTGGaa                    <  1:249825/151‑1 (MQ=255)

                                                                                                                           ggTTGAAGCGCAATCCACAGGGTGCTGCGGCGTCGTTTCGGCACATGTTTTCGAACAAGGTGTGGATGCCGTTCGTCAAGAGCTGGCTCGCTTACAGCAAGAGGGCTACCGCTACGCGGTGCTTGATGCGCTGACCGAACACCATCTGGa                     <  2:644180/150‑1 (MQ=255)

                                                                                                                             ttGAAGCGCAATCCACAGGGTGCTGCGGCGTCGTTTCGGCACATGTTTTCGAACAAGGTGTGGATGCCGTTCGTCAAGAGCTGGCTCGCTTACAGCAAGAGGGCTACCGCTACGCGGTGCTTGATGCGCTGACCGAACACCATCTGGAAAt                  >  1:591251/1‑151 (MQ=255)

                                                                                                                                    gcAATCCACAGGGTGCTGCGGCGTCGTTTCGGCACATGTTTTCGAACAAGGTGTGGATGCCGTTCGTCAAGAGCTGGCTCGCTTACAGCAAGAGGGCTACCGCTACGCGGTGCTTGATGCGCTGACCGAACACCATCTGGAAATTCAggg            >  2:269274/1‑150 (MQ=255)

                                                                                                                                             aGGGTGCTGCGGCGTCGTTTCGGCACATGTTTTCGAACAAGGTGTGGATGCCGTTCGTCAAGAGCTGGCTCGCTTACAGCAAGAGGGCTACCGCTACGCGGTGCTTGATGCGCTGACCGAACACCATCTGGAAATTCAGGGAGAAGCCTTg  <  2:598630/151‑1 (MQ=255)

                                                                                                                                              gggTGCTGCGGCGTCGTTTCGGCACATGTTTTCGAACAAGGTGTGGATGCCGTTCGTCAAGAGCTGGCTCGCTTACAGCAAGAGGGCTACCGCTACGCGGTGCTTGATGCGCTGACCGAACGCCATCTGGAAATTCAGGGAGAAGCCtt   <  1:101502/149‑1 (MQ=255)

CCTGCCGGTCAACGGACGTACGGTTTATCAGGGGTATTTGTTCGTAATGAATCAACTGCTGGCCGAATCCGGGATGCGCCATCACCCGGTAAATCCCATGACCGACAGCTATCTTCCCCGTCTGGTTGAAGCGCAATCCACAGGGCGCTGCGGCGTCGTTTCGGCACATGTTTTCGAACAAGGTGTGGATGCCGTTCGTCAAGAGCTGGCTCGCTTACAGCAAGAGGGCTACCGCTACGCGGTGCTTGATGCGCTGACCGAACACCATCTGGAAATTCAGGGAGAAGCCTTG  >  NC_000913/2862682‑2862973

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A