Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A3 F50 I2 R1	257	49.9	1554294	99.0%	1538751	150.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,923,308	T→C	G159G (GGA→GGG)	truC ←	tRNA(Ile1,Asp) pseudouridine(65) synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,923,308	0	T	C	100.0%	144.6 / NA	44	G159G (GGA→GGG)	truC	tRNA(Ile1,Asp) pseudouridine(65) synthase
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (23/21); total (23/21)

CAATGACAACTGACTGGCATGTAGCATTAACCGCTGGAGGCCAAAATGCTCAGCACCGCTGCGATTCTGGCGTAAATCGCCATGTTTGCTATCGCCAATAATCGGATGACGCAAATGGGCAAGATGTCGGCGAAGCTGATGTTTGCGTCCGGTTTTCGGCTCCAGTTCCACCAGGCCGTAGCGCGTGGTCGGGTAACGTCCGGTCGCTACCGGCATTTCTACGGTCGCCAGACCGCGATAATGCGTCACTGCTGGCTGCGGGCCTTTATCTTCGCGGGCAAATTTATC  >  NC_000913/2923161‑2923448

cAATGACAACTGACTGGCATGTAGCATTAACCGCTGGAGGCCAAAATGCTCAGCACCGCTGCGATTCTGGCGTAAATCGCCATGTTTGCTATCGCCAATAATCGGATGACGCAAATGGGCAAGATGTCGGCGAAGCTGATGTTTGCGccc                                                                                                                                            <  2:609479/150‑1 (MQ=255)

cAATGACAACTGACTGGCATGTAGCATTAACCGCTGGAGGCCAAAATGCTCAGCACCGCTGCGATTCTGGCGTAAATCGCCATGTTTGCTATCGCCAATAATCGGATGACGCAAATGGGCAAGATGTCGGCGAAGCTGATGTTTGCGCCCg                                                                                                                                           >  1:220535/1‑151 (MQ=255)

    gACAACTGACTGGCATGTAGCATTAACCGCTGGAGGCCAAAATGCTCAGCACCGCTGCGATTCTGGCGTAAATCGCCATGTTTGCTATCGCCAATAATCGGATGACGCAAATGGGCAAGATGTCGGCGAAGCTGATGTTTGCGCCCGGtt                                                                                                                                        <  2:247565/150‑1 (MQ=255)

     aCAACTGACTGGCATGTAGCATTAACCGCTGGAGGCCAAAATGCTCAGCACCGCTGCGATTCTGGCGTAAATCGCCATGTTTGCTATCGCCAATAATCGGATGACGCAAATGGGCAAGATGTCGGCGAAGCTGATGTTTGCGCCCGGtttt                                                                                                                                      <  1:537046/151‑1 (MQ=255)

         ctgactgGCATGTAGCATTAACCGCTGGAGGCCAAAATGCTCAGCACCGCTGCGATTCTGGCGTAAATCGCCATGTTTGCTATCGCCAATAATCGGATGACGCAAATGGGCAAGATGTCGGCGAAGCTGATGTTTGCGCCCGGTTTTCgg                                                                                                                                   >  2:597442/1‑150 (MQ=255)

         ctgactgGCATGTAGCATTAACCGCTGGAGGCCAAAATGCTCAGCACCGCTGCGATTCTGGCGTAAATCGCCATGTTTGCTATCGCCAATAATCGGATGACGCAAATGGGCAAGATGTCGGCGAAGCTGATGTTTGCGCCCGGTTTTCGGc                                                                                                                                  >  2:528507/1‑151 (MQ=255)

            actgGCATGTAGCATTAACCGCTGGAGGCCAAAATGCTCAGCACCGCTGCGATTCTGGCGTAAATCGCCATGTTTGCTATCGCCAATAATCGGATGACGCAAATGGGCAAGATGTCGGCGAAGCTGATGTTTGCGCCCGGTTTTCGGc                                                                                                                                  <  1:271932/148‑1 (MQ=255)

                 cATGTAGCATTAACCGCTGGAGGCCAAAATGCTCAGCACCGCTGCGATTCTGGCGTAAATCGCCATGTTTGCTATCGCCAATAATCGGATGACGCAAATGGGCAAGATGTCGGCGAAGCTGATGTTTGCGCCCGGTTTTCGGCTCCAGTTc                                                                                                                          >  2:704085/1‑151 (MQ=255)

                                      ggCCAAAATGCTCAGCACCGCTGCGAATCTGGCGTAAATCGCCATGTTTGCTATCGCCAATAATCGGATGACGCAAATGGGCACGATGTCGGCGAAGCTGATGTTTGCGCCCGGTTTTCGGCTCCAGTTCCACCAGGCCGCAGCGCGTg                                                                                                       >  1:28120/1‑149 (MQ=255)

                                         cAAAATGCTCAGCAGCGCTGCGATTCTGGCGTAAATCGCCATGTTTGCTATCGCCAATAATCGGATGACGCAAATGGGCAAGATGTCGGCGAAGCTGATGTTTGCGCCCGGTTTTCGGCTCCAGTTCCACCAGGCCGTAGCGCGTGGTCgg                                                                                                  <  1:648630/151‑1 (MQ=255)

                                               gCTCAGCACCGCTGCGATTCTGGCGTAAATCGCCATGTTTGCTATCGCCAATAATCGGATGACGCAAATGGGCAAGATGTCGGCGAAGCTGATGTTTGCGCCCGGTTTTCGGCTCCAGTTCCACCAGGCCGTAGCGCGTGGTCGGGTAACg                                                                                            <  1:79937/151‑1 (MQ=255)

                                               gCTCAGCACCGCTGCGATTCTGGCGTAAATCGCCATGTTTGCTATCGCCAATAAGCGGATGACGCAAATGGGCAAGATGTCGGCGAAGCTGATGTTTGCGCCCGGTTTTCGGCTCCAGTTCCACCAGGCCGTAGCGCGTGGTCGGGTAACg                                                                                            <  2:258448/151‑1 (MQ=255)

                                                           tGCGATTCTGGCGTAAATCGCCATGTTTGCTATCGCCAATAATCGGATGACGCAAATGGGCAAGATGTCGGCGAAGCTGATGTTTGCGCCCGGTTTTCGGCTCCAGTTCCACCAGGCCGTAGCGCGTGGTCGGGTAACGTCCGGTCGCTAc                                                                                >  2:361836/1‑151 (MQ=255)

                                                            gCGATTCTGGCGTAAATCGCCATGTTTGCTATCGCCAATAATCGGATGACGCAAATGGGCAAGATGTCGGCGAAGCTGATGTTTGCGCCCGGTTTTCGGCTCCAGTTCCACCAGGCCGTAGCGCGTGGTCGGGTAACGTCCGGTCGCTAcc                                                                               <  2:356340/151‑1 (MQ=255)

                                                            gCGATTCTGGCGTAAATCGCCATGTTTGCTATCGCCAATAATCGGATGACGCAAATGGGCAAGATGTCGGCGAAGCTGATGTTTGCGCCCGGTTTTCGGCTCCAGTTCCACCAGGCCGTAGCGCGTGGTCGGGTAACGTCCGGTCGCTAcc                                                                               >  1:390950/1‑151 (MQ=255)

                                                            gCGATTCTGGCGTAAATCGCCATGTTTGCTATCGCCAATAATCGGATGACGCAAATGGGCAAGATGTCGGCGAAGCTGATGTTTGCGCCCGGTTTTCGGCTCCAGTTCCACCAGGCCGTAGCGCGTGGTCGGGTAACGTCCGGTCGCTAcc                                                                               <  2:332612/151‑1 (MQ=255)

                                                             cGATTCTGGCGGAAATCGCCATGTTTGCTATCGCCAATAAACGGATGACGCAAATGGGCAAGATGTCGGCGAAGCTGATGTTTGCGCTGGGTTTTCGGCTCCAGTTCCCCCAGGCCGTAGCGCGTGGTCGTGTAACGCCCGGTCGCTAcc                                                                               <  1:41900/150‑1 (MQ=255)

                                                               aTTCTGGCGTAAATCGCCATGTTTGCTATCGCCAATAATCGGATGACGCAAATGGGCAAGATGTCGGCGAAGCTGATGTTTGCGCCCGGTTTTCGGCTCCAGTTCCACCAGGCCGTAGCGCGTGGTCGGGTAACGTCCGGTCGCTACCGGc                                                                            >  1:349773/1‑151 (MQ=255)

                                                                          aaTCGCCATGTTTGCTATCGCCAATAATCGGATGACGCAAATGGGGAAGATGTCGGCGAAGCTGATGTTTGCGCCCGGTTTTCGGCTCCAGTTCCACCAGGCCGTAGCGCGTGGTCGGGTAACGTCCGGTCGCTACCGGCATTTCTACGGt                                                                 >  1:75320/1‑151 (MQ=255)

                                                                            tCGCCATGTTTGCTATCGCCAATAATCGGATGACGCAAATGGGCAAGATGTCGGCGAAGCTGATGTTTGCGCCCGGTTTTCGGCTCCAGTTCCACCAGGCCGTAGCGCGTGGTCGGGTAACGTCCGGTCGCTACCGGCATTTCTACGGTCg                                                               >  2:612236/1‑151 (MQ=255)

                                                                              gCCATGTTTGCTATCGCCAATAATCGGATGACGCAAATGGGCAAGATGTCGGCGAAGCTGATGTTTGCGCCCGGTTTTCGGCTCCAGTTCCACCAGGCCGTAGCGCGTGGTCGGGTAACGTCCGGTCGCTACCGGCATTTCTACGGTCGcc                                                             >  1:667531/1‑151 (MQ=255)

                                                                               ccATGTTTGCTATCGCCAATAATCGGATGACGCAAATGGGCAAGATGTCGGCGAAGCTGATGTTTGCGCCCGGTTTTCGGCTCCAGTTCCACCAGGCCGTAGCGCGTGGTCGGGTAACGTCCGGTCGCTACCGGCATTTCTACGGTCGCCa                                                            <  2:555778/151‑1 (MQ=255)

                                                                                       gCTATCGCCAATAATCGGATGACGCAAATGGGCAAGATGTCGGCGAAGCTGATGTTTGCGCCCGGTTTTCGGCTCCAGTTCCACCAGGCCGTAGCGCGTGGTCGGGTAACGTCCGGTCGCTACCGGCATTTCTACGGTCGCCAGACcgcg                                                     >  1:198490/1‑150 (MQ=255)

                                                                                        cTATCGCCAATAATCGGATGACGCAAATGGGCAAGATGTCGGCGAAGCTGATGTTTGCGCCCGGTTTTCGGCTCCAGTTCCACCAGGCCGTAGCGCGTGGTCGGGTAACGTCCGGTCGCTACCGGCATTTCTACGGTCGCCAGACCGCGAt                                                   >  2:313194/1‑151 (MQ=255)

                                                                                         tATCGCCAATAATCGGATGACGCAAATGGGCAAGATGTCGGCGAAGCTGATGTTTGCGCCCGGTTTTCGGCTCCAGTTCCACCAGGCCGTAGCGCGTGGTCGGGTAACGTCCGGTCGCTACCGGCATTTCTACGGTCGCCAGACCGCGAt                                                   >  1:528991/1‑150 (MQ=255)

                                                                                               cAATAATCGGATGACGCAAATGGGCAAGATGTCGGCGAAGCTGATGTTTGCGCCCGGTTTTCGGCTCCAGTTCCACCAGGCCGTAGCGCGTGGTCGGGTAACGTCCGGTCGCTACCGGCATTTCTACGGTCGCCAGACCGCGATAATGCGt                                            <  2:26799/151‑1 (MQ=255)

                                                                                                 ataatCGGATGACGCAAATGGGCAAGATGTCGGCGAAGCTGATGTTTGCGCCCGGTTTTCGGCTCCAGTTCCACCAGGCCGTAGCGCGTGGTCGGGTAACGTCCGGTCGCTACCGGCATTTCTACGGTCGCCAGACCGCGATAATGCGTCa                                          <  2:487150/151‑1 (MQ=255)

                                                                                                    atCGGATGACGCAAATGGGCAAGATGTCGGCGAAGCTGATGTTTGCGCCCGGTTTTCGGCTCCAGTTCCACCAGGCCGTAGCGCGTGGTCGGGTAACGTCCGGTCGCTACCGGCATTTCTACGGTCGCCAGACCGCGATAATGCGTCActg                                       <  1:42352/151‑1 (MQ=255)

                                                                                                      cGGATGACGCAAATGGGCAAGATGTCGGCGAAGCTGATGTTTGCGCCCGGTTTTCGGCTCCAGTTCCACCAGGCCGTAGCGCGTGGTCGGGTAACGTCCGGTCGCTACCGGCATTTCTACGGTCGCCAGACCGCGATAATGCGTCActgct                                     >  1:464214/1‑151 (MQ=255)

                                                                                                            aCGCAAATGGGCAAGATGTCGGCGAAGCTGATGTTTGCGCCCGGTTTTCGGCTCCAGTTCCACCAGGCCGTAGCGCGTGGTCGGGTAACGTCCGGTCGCTACCGGCATTTCTACGGTCGCCAGACCGCGATAATGCGTCACTGCTGGCTGc                               >  2:401457/1‑151 (MQ=255)

                                                                                                             cGCAAATGGGCAAGATGTCGGCGAAGCTGATGTTTGCGCCCGGTTTTCGGCTCCAGTTCCACCAGGCCGTAGCGCGTGGTCGGGTAACGTCCGGTCGCTACCGGCATTTCTACGGTCGCCAGACCGCGATAATGCGTCACTGCTGGCTGCg                              <  2:293159/151‑1 (MQ=255)

                                                                                                                aaaTGGGCAAGATGTCGGCGAAGCTGATGTTTGCGCCCGGTTTTCGGCTCCAGTTCCACCAGGCCGTAGCGCGTGGTCGGGTAACGTCCGGTCGCTACCGGCATTTCTACGGTCGCCAGACCGCGATAATGCGTCACTGCTGGCTGCGGGc                           <  1:201240/151‑1 (MQ=255)

                                                                                                                 aaTGGGCAAGATGTCGGCGAAGCTGATGTTTGCGCCCGGTTTTCGGCTCCAGTTCCACCAGGCCGTAGCGCGTGGTCGGGTAACGTCCGGTCGCTACCGGCATTTCTACGGTCGCCAGACCGCGATAATGCGTCACTGCTGGCTGCGGGcc                          >  1:462769/1‑151 (MQ=255)

                                                                                                                   tGGGCAAGATGTCGGCGAAGCTGATGTTTGCGCCCGGTTTTCGGCTCCAGTTCCACCAGGCCGTAGCGCGTGGTCGGGTAACGTCCGGTCGCTACCGGCATTTCTACGGTCGCCAGACCGCGATAATGCGTCACTGCTGGCTGCGGGCCtt                        >  1:680141/1‑151 (MQ=255)

                                                                                                                      gCAGGATGTCGGCGAAGCTGATGTTTGCGCCCGGTTTTCGGCTCCAGTTCCACCAGGCCGTAGCGCGTGGTCGGGTAACGTCCGGTCGCTACCGGCATTTCTACGGTCGCCAGACCGCGATAATGCGTCACTGCTGGCTGCGGGCCTTTa                      <  1:55768/150‑1 (MQ=255)

                                                                                                                          gATGTCGGCGAAGCTGATGTTTGCGCCCGGTTTTCGGCTCCAGTTCCACCAGGCCGTAGCGCGTGGTCGGGTAACGTCCGGTCGCTACCGGCATTTCTACGGTCGCCAGACCGCGATAATGCGTCACTGCTGGCTGCGGGCCTTTATCTTc                 <  1:117589/151‑1 (MQ=255)

                                                                                                                               cGGCGAAGCTGATGTTTGCGCCCGGTTTTCGGCTCCAGTTCCACCAGGCCGTAGCGCGTGGTCGGGTAACGTCCGGTCGCTACCGGCATTTCTACGGTCGCCAGACCGCGATAATGCGTCACTGCTGGCTGCGGGCCTTTATCTTCGCgg             <  2:146496/150‑1 (MQ=255)

                                                                                                                                ggCGAAGCTGATGTTTGCGCCCGGTTTTCGGCTCCAGTTCCACCAGGCCGTAGCGCGTGGTCGGGTAACGTCCGGTCGCTACCGGCATTTCTACGGTCGCCAGACCGCGATAATGCGTCACTGCTGGCTGCGGGCCTTTATCTTCGCGGGc           >  1:494624/1‑151 (MQ=255)

                                                                                                                                ggCGAAGCTGATGTTTGCGCCCGGTTTTCGGCTCCAGTTCCACCAGGCCGTAGCGCGTGGTCGGGTAACGTCCGGTCGCTACCGGCATTTCTACGGTCGCCAGACCGCGATAATGCGTCACTGCTGGCTGCGGGCCTTTATCTTCGCGGGc           >  2:649596/1‑151 (MQ=255)

                                                                                                                                 gCGAAGCTGATGTTTGCGCCCGGTTTTCGGCTCCAGTTCCACCAGGCCGTAGCGCGTGGTCGGGTAACGTCCGGTCGCTACCGGCATTTCTACGGTCGCCAGACCGCGATAATGCGTCACTGCTGGCTGCGGGCCTTTATCTTCGCGGGCa          <  2:127983/151‑1 (MQ=255)

                                                                                                                                     aGCTGATGTTTGCGCCCGGTTTTCGGCTCCAGTTCCACCAGGCCGTAGCGCGTGGTCGGGTAACGTCCGGTCGCTACCGGCATTTCTACGGTCGCCAGACCGCGATAATGCGTCACTGCTGGCTGCGGGCCTTTATCTTCGCGGGCAAAtt      >  2:648672/1‑151 (MQ=255)

                                                                                                                                         gATGTTTGCGCCCGGTTTTCGGCTCCAGTTCCACCAGGCCGTAGCGCGTGGTCGGGTAACGTCCGGTCGCTACCGGCATTTCTACGGTCGCCAGACCGCGATAATGCGTCACTGCTGGCTGCGGGCCTTTATCTTCGCGGGCAAATTTa    <  1:381696/149‑1 (MQ=255)

                                                                                                                                         gATGTTTGCGCCCGGTTTTCGGCTCCAGTTCCACCAGGCCGTAGCGCGTGGTCGGGTAACGTCCGGTCGCTACCGGCATTTCTACGGTCGCCAGACCGCGATAATGCGTCACTGCTGGCTGCGGGCCTTTATCTTCGCGGGCAAATTTAt   >  2:193179/1‑150 (MQ=255)

                                                                                                                                         gATGTTTGCGCCCGGTTTTCGGCTCCAGTTCCACCAGGCCGTAGCGCGTGGTCGGGTAACGTCCGGTCGCTACCGGCATTTCTACGGTCGCCAGACCGCGATAATGCGTCACTGCTGGCTGCGGGCCTTTATCTTCGCGGGCAAATTTATc  <  2:93327/151‑1 (MQ=255)

                                                                                                                                          aTGTTTGCGCCCGGTTTTCGGCTCCAGTTCCACCAGGCCGTAGCGCGTGGTCGGGTAACGTCCGGTCGCTACCGGCATTTCTACGGTCGCCAGACCGCGATAATGCGTCACTGCTGGCTGCGGGCCTTTATCTTCGCGGGCAAATTTATc  >  1:537437/1‑150 (MQ=255)

CAATGACAACTGACTGGCATGTAGCATTAACCGCTGGAGGCCAAAATGCTCAGCACCGCTGCGATTCTGGCGTAAATCGCCATGTTTGCTATCGCCAATAATCGGATGACGCAAATGGGCAAGATGTCGGCGAAGCTGATGTTTGCGTCCGGTTTTCGGCTCCAGTTCCACCAGGCCGTAGCGCGTGGTCGGGTAACGTCCGGTCGCTACCGGCATTTCTACGGTCGCCAGACCGCGATAATGCGTCACTGCTGGCTGCGGGCCTTTATCTTCGCGGGCAAATTTATC  >  NC_000913/2923161‑2923448

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A