Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A3 F50 I2 R1	257	49.9	1554294	99.0%	1538751	150.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,000,899	A→G	E185E (GAA→GAG)	xdhA →	xanthine dehydrogenase, molybdenum binding subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,000,899	0	A	G	100.0%	129.7 / NA	36	E185E (GAA→GAG)	xdhA	xanthine dehydrogenase, molybdenum binding subunit
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (16/20); total (16/20)

CAGAAGACGCTGCACCAATCCATAACGGTGGCAATTTACTGAAACAAAGCACGATGTCGACGGGTAATGTCCAACAAACAATCGATGCCGCCGACTACCAGGTACAGGGGCACTATCAGACCCCCGTTATTCAACATTGTCACATGGAAAGCGTAACATCGCTGGCGTGGATGGAGGATGACTCGCGAATTACCATCGTTTCCAGCACCCAGATCCCGCACATTGTTCGCCGCGTGGTTGGTCAGGCGCTGGATATTCCCTGGTCATGCGTACGAGTCATCAAACCATTTGTCGG  >  NC_000913/3000751‑3001045

cAGAAGACGCTGCACCAATCCATAACGGTGGCAATTTACTGAAACAAAGCACGATGTCGACGGGTAATGTCCAACAAACAATCGATGCCGCCGACTACCAGGTACAGGGGCACTATCAGACCCCCGTTATTCAACATTGTCACATGGagag                                                                                                                                                  <  1:633772/151‑1 (MQ=255)

 agaagaCGCTGCACCAATCCATAACGGTGGCAATTTACTGAAACAAAGCACGATGTCGACGGGTAATGTCCAACAAACAATCGATGCCGCCGACTACCAGGTACAGGGGCACTATCAGACCCCCGTTATTCAACATTGTCACATGGAGAGc                                                                                                                                                 <  1:251596/151‑1 (MQ=255)

   aagaCGCTGCACCAATCCATAACGGTGGCAATTTACTGAAACAAAGCACGATGTCGACGGGTAATGTCCAACAAACAATCGATGCCGCCGACTACCAGGTACAGGGGCACTATCAGACCCCCGTTATTCAACATTGTCACATGGAGAGCGt                                                                                                                                               <  2:691358/151‑1 (MQ=255)

      aCGCTGCACCAATCCATAACGGTGGCAATTTACTGAAACAAAGCACGATGTCGACGGGTAATGTCCAACAAACAATCGATGCCGCCGACTACCAGGCACAGGGGCACTATCAGACCCCCGTTATTCAACATTGTCACATGGAGAGCGTAAc                                                                                                                                            >  1:499792/1‑151 (MQ=255)

                 aTCCATAACGGTGGCAATTTACTGAAACAAAGCACGATGTCGACGGGTAATGTCCAACAAACAATCGATGCCGCCGACTACCAGGTACAGGGGCACTATCAGACCCCCGTTATTCAACATTGTCACATGGAGAGCGTAACATCGCTGGCGt                                                                                                                                 <  2:324141/151‑1 (MQ=255)

                  tCCATAACGGTGGCAATTTACTGAAACAAAGCACGATGTCGACGGGTAATGTCCAACAAACAATCGATGCCGCCGACTACCAGGTACAGGGGCACTATCAGACCCCCGTTATTCAACATTGTCACATGGAGAGCGTAACATCGCTGGCGt                                                                                                                                 >  2:220694/1‑150 (MQ=255)

                   ccATAACGGTGGCAATTTACTGAAACAAAGCACGATGTCGACGGGTAATGTCCAACAAACAATCGATGCCGCCGACTACCAGGTACAGGGGCACTATCAGACCCCCGTTATTCAACATTGTCACATGGAGAGCGTAACATCGCTGGCGtg                                                                                                                                <  2:213502/150‑1 (MQ=255)

                     aTAACGGTGGCAATTTACTGAAACAAAGCACGATGTCGACGGGTAATGTCCAACAAACAATCGATGCCGCCGACTACCAGGTACAGGGGCACTATCAGACCCCCGTTATTCAACATTGTCACATGGAGAGCGTAACATCGCTGGCGtggat                                                                                                                             >  2:528661/1‑151 (MQ=255)

                       aaCGGTGGCAATTTACTGAAACAAAGCACGATGTCGACGGGTAATGTCCAACAAACAATCGATGCCGCCGACTACCAGGTACAGGGGCACTATCAGACCCCCGTTATTCAACATTGTCACATGGAGAGCGTAACATCGCTGGCGtggatg                                                                                                                            <  2:488913/150‑1 (MQ=255)

                        aCGGTGGCAATTTACTGAAACAAAGCACGATGTCGACGGGTAATGTCCAACAAACAATCGATGCCGCCGACTACCAGGTACAGGGGCACTATCAGACCCCCGTTATTCAACATTGTCACATGGAGAGCGTAACATCGCTGGCGtggatgg                                                                                                                           >  2:300763/1‑150 (MQ=255)

                          ggTGGCAATTTACTGAAACAAAGCACGATGTCGACGGGTAATGTCCAACAAACAATCGATGCCGCCGACTACCAGGTACAGGGGCACTATCAGACCCCCGTTATTCAACATTGTCACATGGAGAGCGTAACATCGCTGGCGTGGATggagg                                                                                                                        >  1:244379/1‑151 (MQ=255)

                                aaTTTACTGAAACAAAGCACGATGTCGACGGGTAATGTCCAACAAACATTCGATGCCGCCGACTACCAGGTACAGGGGCACTATCAGACCCCCGTTATTCAACATTGTCACATGGAGAGCGTAACATCGCTGGCGTGGATGGAGGATGACt                                                                                                                  <  1:298331/151‑1 (MQ=255)

                                      cTGACACAAAGCACGATGTCGAAGGGTAATGTCCAACAAACAATCGATGCCGCCGACTACCAGGCACAGGGGCACTATCAGACCCCCGTTATTCAACATTGTCACATGGAGAGCGTAACATCGCTGGCGTGGATGGAGGATGACTCGCGa                                                                                                             >  1:45873/1‑150 (MQ=255)

                                        gAAACAAAGCACGATGTCGACGGGTAATGTCCAACAAACAATCGATGCCGCCGACTACCAGGTACAGGGGCACTATCAGACCCCCGTTATTCAACATTGTCACATGGAGAGCGTAACATCGCTGGCGTGGATGGAGGATGACTCGCGAAtt                                                                                                          >  1:236525/1‑151 (MQ=255)

                                        gAAACAAAGCACGATGTCGACGGGTAATGTCCAACAAACAATCGATGCCGCCGACTACCAGGTACAGGGGCACTATCAGACCCCCGCTATTCAACATTGTCACATGGCGAGCGTAACATCGCTGGCGTGGTTGGAGGATGACTCGCGAAtt                                                                                                          >  2:328098/1‑151 (MQ=255)

                                          aaCAAAGCACGATGTCGACGGGTAATGTCCAACAAACAATCGATGCCGCCGACTACCAGGTACAGGGGCACTATCAGACCCCCGTTATTCAACATTGTCACATGGAGAGCGTAACATCGCTGGCGTGGATGGAGGATGACTCGCGAATTAc                                                                                                        <  1:401840/151‑1 (MQ=255)

                                                gCACGATGTCGACGGGTAATGTCCAACAAACAATCGATGCCGCCGACTACCAGGTACAGGGGCACTATCAGACCCCCGTTATTCAACATTGTCACATGGAGAGCGTAACATCGCTGGCGTGGATGGAGGATGACTCGCGAATTACCATCGt                                                                                                  <  2:250827/151‑1 (MQ=255)

                                                                   tGTCCAACAAACAATCGATGCCGCCGACTACCAGGTACAGGGGCACTATCAGACCCCCGTTATTCAACATTGTCACATGGAGAGCGTAACATCGCTGGCGTGGATGGAGGATGACTCGCGAATTACCATCGTTTCCAGCACCCAGATCCCg                                                                               >  2:622156/1‑151 (MQ=255)

                                                                   tGTCCAACAAACAATCGATGCCGCCGACTACCAGGTACAGGGGCACTATCAGACCCCCGTTATTCAACATTGTCACATGGAGAGCGTAACATCGCTGGCGTGGATGGAGGATGACTCGCGAATTACCATCGTTTCCAGCACCCAGATCCCg                                                                               >  2:675479/1‑151 (MQ=255)

                                                                      ccAACAAACAATCGATGCCGCCGACTACCAGGTACAGGGGCACTATCAGACCCCCGTTATTCAACATTGTCACATGGAGAGCGTAACATCGCTGGCGTGGATGGAGGATGACTCGCGAATTACCATCGTTTCCAGCACCCAGATCCCGcac                                                                            <  2:205634/151‑1 (MQ=255)

                                                                              caaTCGATGCCGCCGACTACCAGGTACAGGGGCACTATCAGACCCCCGTTATTCAACATTGTCACATGGAGAGCGTAACATCGCTGGCGTGGATGGAGGATGACTCGCGAATTACCATCGTTTCCAGCACCCAGATCCCGCACATTGTTcg                                                                    <  2:60088/151‑1 (MQ=255)

                                                                                  cGATGCCGCCGACTACCAGGTACAGGGGCACTATCAGACCCCCGTTATTCAGCATTGTCACATGGAGAGCGTAACATCGCTGGCGTGGATGGAGGATGACTCGCGAATTACCATCGTTTCCAGCACCCAGATCCCGCACATTGTTcgccgc                                                                <  2:466933/151‑1 (MQ=255)

                                                                                           cgACTACCAGGTACAGGGGCACTATCAGACCCCCGTTATTCAACATTGTCACATGGAGAGCGTAACATCGCTGGCGTGGATGGAGGATGACTCGCGAATTACCATCGTTTCCAGCACCCAGATCCCGCACATTGTTCGCCGCGtggttggt                                                       >  2:592381/1‑151 (MQ=255)

                                                                                                aCCAGGTACAGGGGCACTATCAGACCCCCGTTATTCAACATTGTCACATGGAGAGCGTAACATCGCTGGCGTGGATGGAGGATGACTCGCGAATTACCATCGTTTCCAGCACCCAGATCCCGCACATTGTTCGCCGCGTGGTTGGTCAGgc                                                  >  2:283727/1‑151 (MQ=255)

                                                                                                    ggTACAGGGGCACTATCAGACCCCCGTTATTCAACATTGTCACATGGAGAGCGTAACATCGCTGGCGTGGATGGAGGATGACTCGCGAATTACCATCGTTTCCAGCACCCAGATCCCGCACATTGTTCGCCGCGTGGTTGGTCAGGCGCTg                                              <  2:763933/151‑1 (MQ=255)

                                                                                                       aCAGGGGCACTATCAGACCCCCGTTATTCAACATTGTCACATGGAGAGCGTAACATCGCTGGCGTGGATGGAGGATGACTCGCGAATTACCATCGTTTCCAGCACCCAGATCCCGCACATTGTTCGCCGCGTGGTTGGTCAGGCGCTGGat                                           >  1:37816/1‑151 (MQ=255)

                                                                                                                  aTCAGACCCCCGTTATTCAACATTGTCACATGGAGAGCGTAACATCGCTGGCGTGGATGGAGGATGACTCGCGAATTACCATCGTTTCCAGCACCCAGATCCCGCACATTGTTCGCCGCGTGGTTGGTCAGGCGCTGGATATTCCCTGGTc                                <  1:713392/151‑1 (MQ=255)

                                                                                                                       aCCCCCGTTATTCAACATTGTCACATGGAGAGCGTAACATCGCTGGCGTGGATGGAGGATGACTCGCGAATTACCATCGTTTCCAGCACCCAGATCCCGCACATTGTTCGCCGCGTGGTTGGTCAGGCGCTGGATATTCCCTGGTCATGCg                           <  2:236558/151‑1 (MQ=255)

                                                                                                                             gTTATTCAACATTGTCACATGGAGAGCGTAACATCGCTGGCGTGGATGGAGGATGACTCGCGAATTACCATCGTTTCCAGCACCCAGATCCCGCACATTGTTCGCCGCGTGGTTGGTCAGGCGCTGGATATTCCCTGGTCATGCGTACGAg                     <  1:541793/151‑1 (MQ=255)

                                                                                                                                 ttCAACATTGTCACATGGAGAGCGTAACATCGCTGGCGTGGATGGAGGATGACTCGCGAATTACCATCGTTTCCAGCACCCAGATCCCGCACATTGTTCGCCGCGTGGTTGGTCAGGCGCTGGATATTCCCTGGTCATGCGTAc                        >  1:128655/1‑144 (MQ=255)

                                                                                                                                 ttCAACATTGTCACATGGAGAGCGTAACATCGCTGGCGTGGATGGAGGATGACTCGCGAATTACCATCGTTTCCAGCACCCAGATCCCGCACATTGTTCGCCGCGTGGTTGGTCAGGCGCTGGATATTCCCTGGTCATGCGTAc                        <  2:128688/144‑1 (MQ=255)

                                                                                                                                  tCAACATTGTCACATGGAGAGCGTAACATCGCTGGCGTGGATGGAGGATGACTCGCGAATTACCATCGTTTCCAGCACCCAGATCCCGCACATTGTTCGCCGCGTGGTTGGTCAGGCGCTGGATATTCCCTGGTCATGCGTACGAGtcatc                >  2:553465/1‑151 (MQ=255)

                                                                                                                                      cATTGTCACATGGAGAGCGTAACATCGCTGGCGTGGATGGAGGATGACTCGCGAATTACCATCGTTTCCAGCACCCAGATCCCGCACATTGTTCGCCGCGTGGTTGGTCAGGCGCTGGATATTCCCTGGTCATGCGTACGAGTCATCAAAc            <  2:441517/151‑1 (MQ=255)

                                                                                                                                          gTCACATGGAGAGCGTAACATCGCTGGCGTGGATGGAGGATGACTCGCGAATTACCATCGTTTCCAGCACCCAGATCCCGCACATTGTTCGCCGCGTGGTTGGTCAGGCGCTGGATATTCCCTGGTCATGCGTACGAGTCATCAAACCAt         <  2:586352/150‑1 (MQ=255)

                                                                                                                                              caTGGAGAGCGTAACATCGCTGGCGTGGATGGAGGATGACTCGCGAATTACCATCGTTTCCAGCACCCAGATCCCGCACATTGTTCGCCGCGTGGTTGGTCAGGCGCTGGATATTCCCTGGTCATGCGTACGAGTCATCAAACCATTTGTc    <  2:418703/151‑1 (MQ=255)

                                                                                                                                              caTGGAGAGCGTAACATCGCTGGCGTGGATGGAGGATGACTCGCGAATTACCATCGTTTCCAGCACCCAGATCCCGCACATTGTTCGCCGCGTGGTTGGTCAGGCGCTGGATATTCCCTGGTCATGCGTACGAGTCATCAAACCATTTGTc    >  1:186673/1‑151 (MQ=255)

                                                                                                                                                 ggAGAGCGTAACATCGCTGGCGTGGATGGAGGATGACTCGCGAATTACCATCGTTTCCAGCACCCAGATCCCGCACATTGTTCGCCGCGTGGTTGGTCAGGCGCTGGATATTCCCTGGTCATGCGTACGAGTCATCAAACCATTTGTCgg  <  1:322640/150‑1 (MQ=255)

CAGAAGACGCTGCACCAATCCATAACGGTGGCAATTTACTGAAACAAAGCACGATGTCGACGGGTAATGTCCAACAAACAATCGATGCCGCCGACTACCAGGTACAGGGGCACTATCAGACCCCCGTTATTCAACATTGTCACATGGAAAGCGTAACATCGCTGGCGTGGATGGAGGATGACTCGCGAATTACCATCGTTTCCAGCACCCAGATCCCGCACATTGTTCGCCGCGTGGTTGGTCAGGCGCTGGATATTCCCTGGTCATGCGTACGAGTCATCAAACCATTTGTCGG  >  NC_000913/3000751‑3001045

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A