Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A3 F50 I1 R1	300	54.7	1718999	98.4%	1691495	149.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	982,139	C→T	R365* (CGA→TGA)	ldtD →	murein L,D‑transpeptidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	982,139	0	C	T	100.0%	186.3 / NA	51	R365* (CGA→TGA)	ldtD	murein L,D‑transpeptidase
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (21/30); total (21/30)

CTACGATAATGAACTGGTGGAAGCCGTTAAACGTTTTCAGGCATGGCAAGGATTGGGGGCAGATGGTGCTATTGGCCCGGCAACACGTGACTGGTTAAACGTAACGCCCGCCCAGCGTGCTGGTGTGTTGGCTCTCAACATCCAGCGATTGCGCTTGCTGCCAACAGAGCTTTCTACCGGGATCATGGTTAACATTCCGGCCTATTCGCTGGTCTACTATCAGAACGGCAATCAGGTGCTGGATTCGCGAGTCATTGTCGGTCGCCCCGATCGCAAAACGCCGATGATGAG  >  NC_000913/981994‑982284

cTACGATAATGAACTGGTGGAAGCCGTTAAACGTTTTCAGGCATGGCAAGGATTGGGGGCAGATGGTGCTATTGGCCCGGCAACACGTGACTGGTTAAACGTAACGCCCGCCCAGCGTGCTGGTGTGTTGGCTCTCAACATCCAGTGATTg                                                                                                                                              >  1:830681/1‑151 (MQ=255)

    gATAATGAACTGGTGGAAGCCGTTAAACGTTTTCAGGCATGGCAAGGATTGGGGGCAGATGGTGCTATTGGCCCGGCAACACGTGACTGGTTAAACGTAACGCCCGCCCAGCGTGCTGGTGTGTTGGCTCTCAACATCCAGTGATTGCGCt                                                                                                                                          <  1:186939/151‑1 (MQ=255)

       aaTGAACTGGTGGAAGCCGTTAAACGTTTTCAGGCATGGCAAGGATTGGGGGCAGATGGTGCTATTGGCCCGGCAACACGTGACTGGTTAAACGTAACGCCCGCCCAGCGTGCTGGTGTGTTGGCTCTCAACATCCAGTGATTGCGCTtg                                                                                                                                        <  2:529991/150‑1 (MQ=255)

        aTGAACTGGTGGAAGCCGTTAAACGTTTTCAGGCATGGCAAGGATTGGGGGCAGATGGTGCTATTGGCCCGGCAACACGTGACTGGTTAAACGTAACGCCCGCCCAGCGTGCTGGTGTGTTGGCTCTCAACATCCAGTGATTGCGCTtg                                                                                                                                        <  1:28580/149‑1 (MQ=255)

          gAACTGGTGGAAGCCGTTAAACGTTTTCAGGCATGGCAAGGATTGGGGGCAGATGGTGCTATTGGCCCGGCAACACGTGACTGGTTAAACGTAACGCCCGCCCAGCGTGCTGGTGTGTTGGCTCTCAACATCCAGTGATTGCGCTtgctgc                                                                                                                                    <  2:617077/151‑1 (MQ=255)

           aaCTGGTGGAAGCCGTTAAACGTTTTCAGGCATGGCAAGGATTGGGGGCAGATGGTGCTATTGGCCCGGCAACACGTGACTGGTTAAACGTAACGCCCGCCCAGCGTGCTGGTGTGTTGGCTCTCAACATCCAGTGATTGCGCTTGCTGcc                                                                                                                                   >  1:50877/1‑151 (MQ=255)

            aCTGGTGGAAGCCGTTAAACGTTTTCAGGCATGGCAAGGATTGGGGGCAGATGGTGCTATTGGCCCGGCAACACGTGACTGGTTAAACGTAACGCCCGCCCAGCGTGCTGGTGTGTTGGCTCTCAACATCCAGTGATTGCGCTTGCTGCCa                                                                                                                                  <  1:732695/151‑1 (MQ=255)

                    aaGCCGTTAAACGTTTTCAGGCATGGCAAGGATTGGGGGCAGATGGTGCTATTGGCCCGGCAACACGTGACTGGTTAAACGTAACGCCCGCCCAGCGTGCTGGTGTGTTGGCTCTCAACATCCAGTGATTGCGCTTGCTGCCAACAGAGCt                                                                                                                          <  1:663227/151‑1 (MQ=255)

                     aGCCGTTAAACGTTTTCAGGCATGGCAAGGATTGGGGGCAGATGGTGCTATTGGCCCGGCAACACGTGACTGGTTAAACGTAACGCCCGCCCAGCGTGCTGGTGTGTTGGCTCTCAACATCCAGTGATTGCGCTTGCTGCCAACAGAGCtt                                                                                                                         <  1:727419/151‑1 (MQ=255)

                      gCCGTTAAACGTTTTCAGGCATGGCAAGGATTGGGGGCAGATGGTGCTATTGGCCCGGCAACACGTGACTGGTTAAACGTAACGCCCGCCCAGCGTGCTGGTGTGTTGGCTCTCAACATCCAGTGATTGCGCTTGCTGCCAACAGAGCttt                                                                                                                        <  2:434180/151‑1 (MQ=255)

                            aaaCGTTTTCAGGCATGGCAAGGATTGGGGGCAGATGGTGCTATTGGCCCGGCAACACGTGACTGGTTAAACGTAACGCCCGCCCAGCGTGCTGGTGTGTTGGCTCTCAACATCCAGTGATTGCGCTTGCTGCCAACAGAGCTTTCTACCg                                                                                                                  >  2:832719/1‑151 (MQ=255)

                            aaaCGTTTTCAGGCATGGCAAGGATTGGGGGCAGATGGTGCTATTGGCCCGGCAACACGTGACTGGTTAAACGTAACGCCCGCCCAGCGTGCTGGTGTGTTGGCTCTCAACATCCAGTGATTGCGCTTGCTGCCAACAGAGCTTTCTACCg                                                                                                                  <  2:389595/151‑1 (MQ=255)

                               cGTTTTCAGGCATGGCAAGGATTGGGGGCAGATGGTGCTATTGGCCCGGCAACACGTGACTGGTTAAACGTAACGCCCGCCCAGCGTGCTGGTGTGTTGGCTCTCAACATCCAGTGATTGCGCTTGCTGCCAACAGAGCTTTCTACCGGGa                                                                                                               <  2:854721/151‑1 (MQ=255)

                                gTTTTCAGGGATGGCAAGGATTGGAGGCAGATGGTGCTATTGGCCCGGCAACACGTGACTGGTTAAACGTAACGCCCGCCCAGCGTGCTGGTGTGTTGGCTCTCAACATCCAGTGATTGCGCTTGCTGCCAACAGAGCTTTCTACCGTGa                                                                                                               <  1:692249/150‑1 (MQ=255)

                                gTTTTCAGGCATGGCAAGGATTGGGGGCAGATGGTGCTATTGGCCCGGCAACACGTGACTGGTTAAACGTAACGCCCGCCCAGCGTGCTGGTGTGTTGGCTCTCAACATCCAGTGATTGCGCTCGCTGCCAACAGAGCTTTCTACCGGGAt                                                                                                              <  2:176412/151‑1 (MQ=255)

                                  tttCAGGCATGGCAAGGATTGGGGGCAGATGGTGCTATTGGCCCGGCAACACGTGACTGGTTAAACGTAACGCCCGCCCAGCGTGCTGGTGTGTTGGCTCTCAACATCCAGTGATTGCGCTTGCTGCCAACAGAGCTTTCTACCGGGAt                                                                                                              >  1:541842/1‑149 (MQ=255)

                                  tttCAGGCATGGCAAGGATTGGGGGCAGATGGTGCTATTGGCCCGGCAACACGTGACTGGTTAAACGTAACGCCCGCCCAGCGTGCTGGTGTGTTGGCTCTCAACATCCAGTGATTGCGCTTGCTGCCAACAGAGCTTTCTACCGGGATc                                                                                                             <  1:365774/150‑1 (MQ=255)

                                   ttCAGGCATGGCAAGGATTGGGGGCAGATGGTGCTATTGGCCCGGCAACACGTGACTGGTTAAACGTAACGCCCGCCCAGCGTGCTGGTGTGTTGGCTCTCAACATCCAGTGATTGCGCTTGCTGCCAACAGAGCTTTCTACCGGGATCa                                                                                                            <  2:424016/150‑1 (MQ=255)

                                    tCAGGCATGGCAAGGATTGGGGGCAGATGGTGCTATTGGCCCGGCAACACGTGACTGGTTAAACGTAACGCCCGCCCAGCGTGCTGGTGTGTTGGCTCTCAACATCCAGTGATTGCGCTTGCTGCCAACAGAGCTTTCTACCGGGATCAt                                                                                                           <  1:340635/150‑1 (MQ=255)

                                      aGGCATGGCAAGGATTGGGGGCAGATGGTGCTATTGGCCCGGCAACACGTGACTGGTTAAACGTAACGCCCGCCCAGCGTGCTGGTGTGTTGGCTCTCAACATCCAGTGATTGCGCTTGCTGCCAACAGAGCTTTCTACCGGGATCATGGt                                                                                                        >  2:172856/1‑151 (MQ=255)

                                          aTGGCAAGGATTGGGGGCAGATGGTGCTATTGGCCCGGCAACACGTGACTGGTTAAACGTAACGCCCGCCCAGCGTGCTGGTGTGTTGGCTCTCAACATCCAGTGATTGCGCTTGCTGCCAACAGAGCTTTCTACCGGGATCATGGTTAAc                                                                                                    >  1:359692/1‑151 (MQ=255)

                                               aaGGATTGGGGGCAGATGGTGCTATTGGCCCGGCAACACGTGACTGGTTAAACGTAACGCCCGCCCAGCGTGCTGGTGTGTTGGCTCTCAACATCCAGTGATTGCGCTTGCTGCCAACAGAGCTTTCTACCGGGATCATGGTTAACATTcc                                                                                               <  1:565787/151‑1 (MQ=255)

                                               aaGGATTCGGGGCAGATGGTGCTATTGTCCCGGCAACACGTGACTGGTTAAACGTAACGCCCGCCCAGCGTGCTGGTGTGTTGGCTCTCAACATCCAGTGATTGCGCTTGCTGCCAACAGAGCTTTCTACCGGGATCATGGTTAACATTcc                                                                                               >  1:182313/1‑151 (MQ=255)

                                                aGGATTGGGGGCAGATGGTGCTATTGGCCCGGCAACACGTGACTGGTTAAACGTAACGCCCGCCCAGCGTGCTGGTGTGTTGGCTCTCAACATCCAGTGATTGCGCTTGCTGCCAACAGAGCTTTCTACCGGGATCATGGTTAACATTCCg                                                                                              <  2:220149/151‑1 (MQ=255)

                                                 ggATTGGGGGCAGATGGTGCTATTGGCCCGGCAACACGTGACTGGTTAAACGTAACGCCCGCCCAGCGTGCTGGTGTGTTGGCTCTCAACATCCAGTGATTGCGCTTGCTGCCAACAGAGCTTTCTACCGGGATCATGGTTAACATTCCgg                                                                                             >  2:803120/1‑151 (MQ=255)

                                                     tGGGGGCAGATGGTGCTATTGGCCCGGCAACACGTGACTGGTTAAACGTAACGCCCGCCCAGCGTGCTGGTGCGTTGGCTCTCAACATCCAGTGATTGCGCTTGCTGCCAACAGAGCTTTCTACCGGGATCATGGTTAACATTCCGGCCt                                                                                          >  2:754700/1‑150 (MQ=255)

                                                      gggggCAGATGGTGCTATTGGCCCGGCAACACGTGACTGGTTAAACGTAACGCCCGCCCAGCGTGCTGGTGTGTTGGCTCTCAACATCCAGTGATTGCGCTTGCTGCCAACAGAGCTTGCTACCGGGATCATGGTTAACATTCCGGCCTa                                                                                         <  1:316022/150‑1 (MQ=255)

                                                        gggCAGATGGTGCTATTGGCCCGGCAACACGTGACTGGTTAAACGTAACGCCCGCCCAGCGTGCT‑GTGTGTTGGCTCTCAACATCCAGTGATTGCGCTTGCTGCCAACAGAGCTTTCTACCGGGATCATGGTTAACATTCCGGCCTATTCg                                                                                     >  2:687382/1‑151 (MQ=255)

                                                         ggCAGATGGTGCTATTGGCCCGGCAACACGTGACTGGTTAAACGTAACGCCCGCCCAGCGTGCTGGTGTGTTGGCTCTCAACATCCAGTGATTGCGCTTGCTGCCAACAGAGCTTTCTACCGGGATCATGGTTAACATTCCGGCCTATTCg                                                                                     <  2:483526/151‑1 (MQ=255)

                                                                        tGGCCCGGCAACACGTGACTGGTTAAACGTAACGCCCGCCCAGCGTGCTGGTGTGTTGGCTCTCAACATCCAGTGATTGCGCTTGCTGCCAACAGAGCTTTCTACCGGGATCATGGTTAACATTCCGGCCTATTCGCTGGTCTACTATc                                                                        >  2:701735/1‑149 (MQ=255)

                                                                           cccGGCAACACGTGACTGGTTAAACGTAACGCCCGCCCAGCGTGCTGGTGTGTTGGCTCTCAACATCCAGTGATTGCGCTTGCTGCCAACAGAGCTTTCTACCGGGATCATGGTTAACATTCCGGCCTATTCGCTGGTCTACTATCAGAAc                                                                   >  2:221271/1‑151 (MQ=255)

                                                                            ccGGCAACACGTGACTGGTTAAACGTAACGCCCGCCCAGCGTGCTGGTGTGTTGGCTCTCAACATCCAGTGATTGCGCTTGCTGCCAACAGAGCTTTCTACCGGGATCATGGTTAACATTCCGGCCTATTCGCTGGTCTACTATCAGAACg                                                                  >  2:455708/1‑151 (MQ=255)

                                                                            ccGGCAACACGTGACTGGTTAAACGTAACGCCCGCCCAGCGTGCTGGTGTGTTGGCTCTCAACATCCAGTGATTGCGCTTGCTGCCAACAGAGCTTTCTACCGGGATCATGGTTAACATTCCGGCCTATTCGCTGGTCTACTATCAGAACg                                                                  >  1:29896/1‑151 (MQ=255)

                                                                               gCAACACGTGACTGGTTAAACGTAACGCCCGCCCAGCGTGCTGGTGTGTTGGCTCTCAACATCCAGTGATTGCGCTTGCTGCCAACAGAGCTTTCTACCGGGATCATGGTTAACATTCCGGCCTATTCGCTGGTCTACTATCAGAACGGc                                                                >  2:536119/1‑150 (MQ=255)

                                                                                    acGTGACTGGTTAAACGTAACGCCCGCCCAGCGTGCTGGTGTGTTGGCTCTCAACATCCAGTGATTGCGCTTGCTGCCAACAGAGCTTTCTACCGGGATCATGGTTAACATTCCGGCCTATTCGCTGGTCTACTATCAGAACGGCAATc                                                            >  1:144342/1‑149 (MQ=255)

                                                                                    acGTGACTGGTTAAACGTAACGCCCGCCCAGCGTGCTGGTGTGTTGGCTCTCAACATCCAGTGATTGCGCTTGCTGCCAACAGAGCTTTCTACCGGGATCATGGTTAACATTCCGGCCTATTCGCTGGTCTACTATCAGAACGGCAATCAg                                                          <  2:305061/151‑1 (MQ=255)

                                                                                         aCTGGTTAAACGTAACGCCCGCCCAGCGTGCTGGTGTGTTGGCTCTCAACATCCAGTGATTGCGCTTGCTGCCAACAGAGCTTTCTACCGGGATCATGGTTAACATTCCGGCCTATTCGCTGGTCTACTATCAGAACGGCAATCAGGTGc                                                      >  1:85314/1‑150 (MQ=255)

                                                                                          cTGGTTAAACGTAACGCCCGCCCAGCGTGCTGGTGTGTTGGCTCTCAACATCCAGTGATTGCGCTTGCTGCCAACAGAGCTTTCTACCGGGATCATGGTTAACATTCCGGCCTATTCGCTGGTCTACTATCAGAACGGCAATCAGGTGCTg                                                    >  1:625242/1‑151 (MQ=255)

                                                                                            ggTTAAACGTAACGCCCGCCCAGCGTGCTGGTGTGTTGGCTCTCAACATCCAGTGATTGCGCTTGCTGCCAACAGAGCTTTCTACCGGGATCATGGTTAACATTCCGGCCTATTCGCTGGTCTACTATCAGAACGGCAATCAGGTGCTGGa                                                  <  1:2400/151‑1 (MQ=255)

                                                                                              ttAAACGTAACGACCGCCCAGCGTGCTGGTGTGTTGGCTCTCAACATCCAGTGATTGCGCTTGCTGCCATCAGAGCTTTCTCCCGGGATCATGGTTACCATTCCGGCCTATTCGCTGATCTACTATCAGAACGGCAATCAGGTg                                                       <  1:514240/144‑1 (MQ=255)

                                                                                              ttAAAAGTAACGCCCGAACAGCGTGCTGGTGTGTTGGCTCTCAACATCCAGTGATTGCGCTTGCTGCCAACAGAGCTTTCTACCGGGATCATGGTTAACATTCCGGCCTATTCGCTGGTCTACTATCAGAACGGCAATCAGGTGCTGGAtt                                                >  1:106683/1‑151 (MQ=255)

                                                                                                   cGTAACGCCCGCCCAGCGTGCTGGTGTGTTGGCTCTCAACATCCAGTGATTGCGCTTGCTGCCAACAGAGCTTTCTACCGGGATCATGGTTAACATTCCGGCCTATTCGCTGGTCTACTATCAGAACGGCAATCAGGTGCTGGATTcgcg                                            >  1:93158/1‑150 (MQ=255)

                                                                                                      aaCGCCCGCCCAGCGTGCTGGTGTGTTGGCTCTCAACATCCAGTGATTGCGCTTGCTGCCAACAGAGCTTTCTACCGGGATCATGGTTAACATTCCGGCCTATTCGCTGGTCTACTATCAGAACGGCAATCAGGTGCTGGATTCGCGAGTc                                        <  2:833424/151‑1 (MQ=255)

                                                                                                                 aGCGTGCTGGTGTGTTGGCTCTCAACATCCAGTGATTGCGCTTGCTGCCAACAGAGCTTTCTACCGGGATCATGGTTAACATTCCGGCCTATTCGCTGGTCTACTATCAGAACGGCAATCAGGTGCTGGATTCGCGAGTCATTgtcggtcg                             <  2:859452/151‑1 (MQ=255)

                                                                                                                          gtgtgtTGGCTCTCAACATCCAGTGATTGCGCTTGCTGCCAACAGAGCTTTCTACCGGGATCATGGTTAACATTCCGGCCTATTCGCTGGTCTACTATCAGAACGGCAATCAGGTGCTGGATTCGCGAGTCATTGTCGGTCGCCCCGATCg                    <  1:475570/151‑1 (MQ=255)

                                                                                                                            gtgtTGGCTCTCAACATCCAGTGATTGCGCTTGCTGCCAACAGAGCTTTCTACCGGGATCATGGTTAACATTCCGGCCTATTCGCTGGTCTACTATCAGAACGGCAATCAGGTGCTGGATTCGCGAGTCATTGTCGGTCGCCCCGATCGc                   <  1:651907/150‑1 (MQ=255)

                                                                                                                            gtgtTGGCTCTCAACATCCAGTGATTGCGCTTGCTGCCAACAGAGCTTTCTACCGGGATCATGGTTAACATTCCGGCCTATTCGCTGGTCTACTATCAGAACGGCAATCAGGTGCTGGATTCGCGAGTCATTGTCGGTCGCCCCGATCGc                   <  2:721876/150‑1 (MQ=255)

                                                                                                                                tGGCTCTCAACATCCAGTGATTGCGCTTGCTGCCAACAGAGCTTTCTACCGGGATCATGGTTAACATTCCGGCCTATTCGCTGGTCTACTATCAGAACGGCAATCAGGTGCTGGATTCGCGAGTCATTGTCGGTCGCCCCGATCGCAAAAc              >  2:781193/1‑151 (MQ=255)

                                                                                                                                  gCTCTCAACATCCAGTGATTGCGCTTGCTGCCAACAGAGCTTTCTACCGGGATCATGGTTAACATTCCGGCCTATTCGCTGGTCTACTATCAGAACGGCAATCAGGTGCTGGATTCGCGAGTCATTGTCGGTCGCCCCGATCGCAAAACGc            <  1:174225/151‑1 (MQ=255)

                                                                                                                                       cAACATCCAGTGATTGCGCTTGCTGCCAACAGAGCTTTCTACCGGGATCATGGTTAACATTCCGGCCTATTCGCTGGTCTACTATCAGAACGGCAATCAGGTGCTGGATTCGCGAGTCATTGTCGGTCGCCCCGATCGCAAAACGCCgat        <  1:86779/150‑1 (MQ=255)

                                                                                                                                             ccAGTGATTGCGCTTGCTGCCAACAGAGCTTTCTACCGGGATCATGGTTAACATTCCGGCCTATTCGCTGGTCTACTATCAGAACGGCAATCAGGTGCTGGATTCGCGAGTCATTGTCGGTCGCCCCGATCGCAAAACGCCGATGATGAg  <  1:473284/150‑1 (MQ=255)

CTACGATAATGAACTGGTGGAAGCCGTTAAACGTTTTCAGGCATGGCAAGGATTGGGGGCAGATGGTGCTATTGGCCCGGCAACACGTGACTGGTTAAACGTAACGCCCGCCCAGCGTGCTGGTGTGTTGGCTCTCAACATCCAGCGATTGCGCTTGCTGCCAACAGAGCTTTCTACCGGGATCATGGTTAACATTCCGGCCTATTCGCTGGTCTACTATCAGAACGGCAATCAGGTGCTGGATTCGCGAGTCATTGTCGGTCGCCCCGATCGCAAAACGCCGATGATGAG  >  NC_000913/981994‑982284

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A