Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A3 F50 I1 R1	300	54.7	1718999	98.4%	1691495	149.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,072,718	A→G	V49A (GTC→GCC)	rutB ←	ureidoacrylate amidohydrolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,072,718	0	A	G	96.9%	97.2 / NA	32	V49A (GTC→GCC)	rutB	ureidoacrylate amidohydrolase
Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (14/17); minor base T (0/1); total (14/18)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GGGCGTTCGATTTATGAAAATTCGGTGAGCCGGGTCCGCCAGCCTCGACATACTGTTCATCCCAGCCATTTTGAAACCAGATGATCAGCATCCCTGCCGCTCGCGCTGCGGTCACGGCGGTTTGAATGTTGGCAATGACCGGGCGAGTGGTTGAGACATCAAACCCGGCGAGATCTAAGTAGCCGCCTGGCGTGGCATAAGCGTTTTGCATATCCACCACGATCAGCGCACTTTGCTGCGGATCGAAGGTAATGGCTTCCGGTCGAGCGGTTAAGGTCG  >  NC_000913/1072581‑1072859

gggCGTTCGATTTATGAAAATTCGGTGAGCCGGGTCCGCCAGCCTCGACATACTGTTCATCCCAGCCATTTTGAAACCAGATGATCAGCATCCCTGCCGCTCGCGCTGCGGTCACGGCGGTTTGAATGTTGGCAATGGCCGGGCGAGTg                                                                                                                                    <  2:16444/149‑1 (MQ=255)

gggCGTTCGATTTATGAAAATTCGGTGAGCCGGGTCCGCCAGCCTCGACATACTGTTCATCCCAGCCATTTTGAAACCAGATGATCAGCATCCCTGCCGCTCGCGCTGCGGTCACGGCGGTTTGAATGTTGGCAATGGCCGGGCGAGTGGt                                                                                                                                  <  1:299259/151‑1 (MQ=255)

 ggCGTTCGATTTATGAAAATTCGGTGAGCCGGGTCCGCCAGCCTCGACATACTGTTCATCCCAGCCATTTTGAAACCAGATGATCAGCATCCCTGCCGCTCGCGCTGCGGTCACGGCGGTTTTAATGTTGGCAATGGCCcggggggggg                                                                                                                                   >  2:412199/1‑142 (MQ=255)

     ttCGATTTATGAAAATTCGGTGAGCCGGGTCCGCCAGCCTCGACATACTGTTCATCCCAGCCATTTTGAAACCAGATGATCAGCATCCCTGCCGCTCGCGCTGCGGTCACGGCGGTTTGAAAGTTGGCAATGGCCgggggggggg                                                                                                                                   >  2:252681/1‑138 (MQ=255)

        gATTTATGAAAATTCGGTGAGCCGGGTCCGCCAGCCTCGACATACTGTTCATCCCAGCCATTTTGAAACCAGATGATCAGCATCCCTGCCGCTCGCGCTGCGGTCACGGCGGTTTGAATGTTGGCAATGGCCGGGCGAGTGGTTGAGACa                                                                                                                           <  2:805133/150‑1 (MQ=255)

             aTGAAAATTCGGTGAGCCGGGTCCGCCAGCCTCGACATACTGTTCATCCCAGCCATTTTGAAACCAGATGATCAGCATCCCTGCCGCTCGCGCTGCGGTCACGGCGGTTTGAATGTTGGCAATGGCCGGGCGAGTGGTTGAGACATCAAAc                                                                                                                     <  2:519172/151‑1 (MQ=255)

                   aTTCGGTGAGCCGGGTCCGCCAGCCTCGACATACTGTTCATCCCAGCCATTTTGAAACCAGATGATCAGCATCCCTGCCGCTCGCGCTGCGGTCACGGCGGTTTTTATTTTTGCAATGGCCGGGGGGGTGGGTGGGAAATCAAAacc                                                                                                                   >  1:746335/1‑147 (MQ=255)

                      cGGTGAGCCGGGTCCGCCAGCCTCGACATACTGTTCATCCCAGCCATTTTGAAACCAGATGATCAGCATCCCTGCCGCTCGCGCTGCGGTCACGGCGGTTTGAATGTTGGCAATGGCCGGGCGAGTGGTTGAGACATCAAACCCGGCgag                                                                                                             <  2:410914/150‑1 (MQ=255)

                                       cAGCCTCGACATACTGTTCATCCCAGCCATTTTGAAACCAGATGATCAGCATCCCTGCCGCTCGCGCTGCGGTCACGGCGGTTTGAATGTTTGCCATGGCCGGGCGAGTGGTTGAGACATCAAACCCCGCGAGATCTAAGTAgccgcc                                                                                              >  2:190778/1‑148 (MQ=255)

                                        aGCCTCGACATACTGTTCATCCCAGCCATTTTGAAACCAGATGATCAGCATCCCTGCCGCTCGCGCTGCGGTCACGGCGGTTTGAATTTTGGCAATGGGCGGGGGGGTGGGTGAGACATCAAACCCCGCGAGATCTAAGTAGCCGCCggg                                                                                           >  2:783637/1‑150 (MQ=255)

                                                cATGCTGTTCACCCCAGCCATTTTGAAACCAGATGATCAGCATCCCTGCCGCTCGCGCTGCGGTCACGGCGGTTTGAATGTTGGCAATGGCCGGGCGAGTGGTTGAGACATCAAACCCGGCGAGATCTAAGTAGCCGCCTGGCGTGGCATa                                                                                  <  1:56824/151‑1 (MQ=255)

                                                cATACTGTTCATCCCAGCCATTTTGAAACCAGATGATCAGCATCCCTGCCGCTCGCGCTGCGGTCACGGCGGTTTGGATGTTGGCAATGGCCGGGCGCGGGGGTGGGACATCAAACCCCGCGGGGGCTAAATAGGCGCCTgggggg                                                                                       >  1:149807/1‑142 (MQ=255)

                                                           tCCCAGCCATTTTGAAACCAGATGATCAGCATCCCTGCCGCTCGCGCTGCGGTCACGGCGGTTTTAAAGGTGGCAACGGCGGGGGGGGAGGGTGAGGCATCAAACCCAGGGAGATCTTAGGAGGCGCCCTGCGGGGCATAAGGGGTTTg                                                                         >  1:764627/1‑149 (MQ=255)

                                                              cAGCCATTTTGAAACCAGATGATCAGCATCCCTGCCGCTCGCGCTGCGGTCACGGCGGTTTGAATGTTGGCAATGGCCGGGCGAGTGGTTGAGACATCAAACCCGGCGAGATCTAAGTAGCCGCCTGGCGTGGCATAAGCGTTTTGCatat                                                                    <  2:356236/151‑1 (MQ=255)

                                                                 ccATTTTGAAACAAGATGATCAGCATCCCTGCCGCTCGCGCTGCGGTCACGGCGGTTTGAATGTTGGCAATGGCCGGGCGAGTGGTTGAGACATCAAACCCGGCGAGATCTAAGTAGCCGCCTGGCGTGGCATAAGCGTTTTg                                                                         <  2:363784/143‑1 (MQ=255)

                                                                   aTTTTGAAACCAGATGATCAGCATCCCTGCCGCTCGCGCTGCGGTCACGGCGGTTTGAATGTTGGCAATGGCCGGGCGAGTGGTTGAGACATCAAACCCGGCGAGATCTAAGTAGCCGCCTGGCGTGGCATAAGCGTTTTGCATATccacc                                                               <  1:783581/151‑1 (MQ=255)

                                                                       tGAAACCAGATGATCAGCATCCCTGCCGCTCGCGCTGCGGTCACGGCGGTTTGAATGTTGGCAATGGCCGGGCGAGGGGGTGAGACATCAAACCCGGCGAGATCTAAGTAGCCGCCCGGCGTGGCATAAGCGTTTTTCATATccacc                                                               >  2:461177/1‑147 (MQ=255)

                                                                             cAGATGATCAGCATCCCTGCCGCTCGCGCTGCGGTCACGGCGGTTTGAATGTTGGCAATGGCCGGGGGGGGGGTTGAGACATCAAAACCGGCGAGATCTAAGTAACCGCCTGGCGTGGGATAAGCGGTTTGCATATccacc                                                               >  2:730024/1‑141 (MQ=255)

                                                                              aGATGATCAGCATCCCTGCCGCTCGCGCTGCGGTCACGGCGGTTTGAATGTTGGCAATGGCCGGGCGAGTGGTTGAGACATCAAACCCGGCGAGATCTAAGTAGCCGCCTGGCGTGGCATAAGCGTTTTGCATATCCACCACGATCAgcgc                                                    <  2:559844/151‑1 (MQ=255)

                                                                              aGATGATCAGCATCCCTGCCGCTCGCGCTGCGGTCACGGCGGTTTGAATGTTGGCAATGGCCGGGCGAGTGGTTGAGACATCAAACCCGGCGAGATCTAAGTAGCCGCCTGGCGTGGCATAAGCGTTTTGCATATCCACCACGATCAgcg                                                     <  1:461141/150‑1 (MQ=255)

                                                                                atgatCAGCATCCCTGCCGCTCGCGCTGCGGTCACGGCGGTTTTAATGTTGGCAATGGGCGGGCGGGGGGGTGGGACATCAAAACCCGCGGGATCTAAGGAGCCGCCCGGCGGGGGATAAAGGGTTTTCATATCCACCACGATCAgcgccc                                                  >  2:535024/1‑149 (MQ=255)

                                                                                   atCAGCATCCCTGCCGCTCGCGCTGCGGTCACGGCGGTTTGAATGTTGGCAATGGCCGGGCGAGTGGTTGAGACATCAAACCCGGCGAGATCTAAGTAGCCGCCTGGCGTGGCATAAGCGTTTTGCATATCCACCACGATCAGCGCACttt                                               <  2:577385/151‑1 (MQ=255)

                                                                                     cAGCATCCCTGCCGCTCGCGCTGCGGTCACGGCGGTTTGAATGTTGGCAATGGCCGGGCGAGTGGTTGAGACATCAAACCCGGCGAGATCTAAGTAGCCGCCTGGCGTGGCATAAGCGTTTTGCATATCCACCACGATCAGCGCACTTtg                                              <  1:815315/150‑1 (MQ=255)

                                                                                      aGCATCCCTGCCGCTCGCGCTGCGGTCACGGCGGTTTTTATGTTGGCCAAGGCCGGGCGGGGGGGTGAGACATCAAACCCGGCGGGATCTAAGTAGGCGCCTGGCGGGGCATAAGAGGTTTGCATATCCACCACGATCAGGGCACttt                                               >  2:181436/1‑148 (MQ=255)

                                                                                           cccTGCCGCTCGCGCTGCGGTCACGGCGGTTTGAATGTTGGCAATGGCCGGGCGAGTGGTTGAGACATCAAACCCGGCGAGATCTAAGTAGCCGCCTGGCGTGGCATAAGCGTTTTGCATATCCACCACGATCAGCGCACTTTGCTGCGGa                                       <  1:343830/151‑1 (MQ=255)

                                                                                              tGCCGCTCGCGCTGCGGTCACGGCGGTTTGGATGTTGGCAATGGCCGGGCGAGCGGTTGAGACACCAAACCCGGCGAGGTCTAAGTAGCCGCCTTGCGTGGCCTAAGGGTTTTTCATATCCACCACGATCAGCGCCCTTTTCTGCGGACCg                                    >  2:566976/1‑151 (MQ=255)

                                                                                                  gCTCGCGCTGCGGTCACGGCGGTTTGAATGTTGGCAATGGCCGGGCGAGTGGTTGAGACATCAAACCCGGCGAGATCTAAGTAGCCGCCTGGCGTGGCATAAGCGTTTTGCATATCCACCACGATCAGCGCACTTTGCTGCGGATCGAAgg                                <  2:425227/151‑1 (MQ=255)

                                                                                                       cgcTGCGGTCACGGCGGTTTGAATGTTGGCAATGGCCGGGCGAGTGGTTGAGACATCAAACCCGGCGAGATCTAAGTAGCCGCCTGGCGTGGCATAAGCGTTTTGCATATCCACCACGATCAGCGCACTTTGCTGCGGATCGAAGGTAAt                            <  2:193350/150‑1 (MQ=255)

                                                                                                               tCACGGTGGTTTCAATGTTGGCAATGTCCGGGCGAGTGGTTGAGACATCAAACCCGGCCAGATCTAAGTAGCCGCCTGGCGTGGCATAAGCGTTTTGCATATCCTCCACGACCAGCGCCCTTTGCTGCGGATCGAAGGTAATGTCTTcc                     <  1:295588/149‑1 (MQ=255)

                                                                                                                       gTTTGAATGTTGGCAATGGCCGGGCGAGTGGTTGAGACATCAAACCCGGCGAGATCTAAGTAGCCGCCTGGCGTGGCATAAGCGTTTTGCATATCCACCACGATCAGCGCACTTTGCTGCGGATCGAAGGTAATGGCTTCCGGTCGAGCgg           >  1:685606/1‑151 (MQ=255)

                                                                                                                               gTTGGCAATGGCCGGGCGAGTGGTTGAGACATCAAACCCGGCGAGATCTAAGTAGCCGCCTGGCGTGGCATAAGCGTTTTGCATATCCACCACGATCAGCGCACTTTGCTGCGGATCGAAGGTAATGGCTTCCGGTCGAGCGGTTAAGgtc   <  2:162563/151‑1 (MQ=255)

                                                                                                                                ttGGCAATGGCCGGGCGAGTGGTTGAGACATCAAACCCGGCGAGATCTAAGTAGCCGCCTGGCGTGGCATAAGCGTTTTGCATATCCACCACGATCAGCGCACTTTGCTGCGGATCGAAGGTAATGGCTTCCGGTCGAGCGGTTAAGgtcg  >  2:688159/1‑151 (MQ=255)

GGGCGTTCGATTTATGAAAATTCGGTGAGCCGGGTCCGCCAGCCTCGACATACTGTTCATCCCAGCCATTTTGAAACCAGATGATCAGCATCCCTGCCGCTCGCGCTGCGGTCACGGCGGTTTGAATGTTGGCAATGACCGGGCGAGTGGTTGAGACATCAAACCCGGCGAGATCTAAGTAGCCGCCTGGCGTGGCATAAGCGTTTTGCATATCCACCACGATCAGCGCACTTTGCTGCGGATCGAAGGTAATGGCTTCCGGTCGAGCGGTTAAGGTCG  >  NC_000913/1072581‑1072859

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A