Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A3 F50 I1 R1	300	54.7	1718999	98.4%	1691495	149.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,238,031	T→C	I154T (ATC→ACC)	dadA →	D‑amino acid dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,238,031	0	T	C	100.0%	146.0 / NA	42	I154T (ATC→ACC)	dadA	D‑amino acid dehydrogenase
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (18/24); total (18/24)

CGGATGGTGCGTCTGGCGGAATACAGCCGTGATTGCCTGAAAGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGACGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGACCCGCGATATCGCCGTGCTGGAAGATGCCGGCGTACCGTATCAGCTGCTGGAATCCAGCCGCCTGGCGGAAGTGGAGCCCGCGCTGGCAGAAGTGGCGCACAAACTGACGGGCGGCCTGCAGTTACCCAATGATGAAACCGGAGACTGTCA  >  NC_000913/1237889‑1238172

cGGATGGTGCGTCTGGCGGAATACAGCCGTGATTGCCTGAAAGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGACGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGACCCGCGATACCGCCGTGc                                                                                                                                       <  1:171328/151‑1 (MQ=255)

 ggATGGTGCGTCTGGCGGAATACAGCCGTGATTGCCTGAAAGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGACGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGACCCGCGATACCGCCGTGCt                                                                                                                                      <  2:270730/151‑1 (MQ=255)

  gATGGTGCGTCTGGCGGAATACAGCCGTGATTGCCTGAAAGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGACGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGACCCGCGATACCGCCGTGCTg                                                                                                                                     <  2:556389/151‑1 (MQ=255)

  gATGGTGCGTCTGGCGGAATACAGCCGTGATTGCCTGAAAGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGACGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGACCCGCGATACCGCCGTGCTg                                                                                                                                     >  2:48218/1‑151 (MQ=255)

    tGGTGCGTCAGGCGGAATACAGCCGTGATTGCCTGAAAGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGACGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGACCCGCGATACCGCCGTGCt                                                                                                                                      <  2:545711/148‑1 (MQ=255)

             tGGCGGAATACAGCCGTGATTGCCTGAAAGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGACGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGACCCGCGATACCGCCGTGCTGGAAGATGCCgg                                                                                                                          >  1:85612/1‑151 (MQ=255)

              ggCGGAATACAGCCGTGATTGCCTGAAAGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGACGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGACCCGCGATACCGCCGTGCTGGAAGATGCCgg                                                                                                                          <  2:829266/150‑1 (MQ=255)

              ggCGGAATACAGCCGTGATTGCCTGAAAGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGACGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGACCCGCGATACCGCCGTGCTGGAAGATGCCGGc                                                                                                                         <  2:306342/151‑1 (MQ=255)

                  gAATACAGCCGTGATTGCCTGAAAGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGACGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGACCCGCGATACCGCCGTGCTGGAAGATGCCGGCGTAc                                                                                                                     >  2:360859/1‑151 (MQ=255)

                    aTACAGCCGTGATTGCCTGAAAGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGACGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGACCCGCGATACCGCCGTGCTGGAAGATGCCGGCGTACCg                                                                                                                   >  2:321280/1‑151 (MQ=255)

                            gTGATTGCCTGAAAGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGACGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGACCCGCGATACCGCCGTGCTGGAAGATGCCGGCGTACCGt                                                                                                                  >  2:859441/1‑144 (MQ=255)

                            gTGATTGCCTGAAAGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGACGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGACCCGCGATACCGCCGTGCTGGAAGATGCCGGCGTACCGt                                                                                                                  <  1:859382/144‑1 (MQ=255)

                              gATTGCCTGAAAGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGACGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGACCCGCGATACCGCCGTGCTGGAAGATGCCGGCGTACCGTATCAgctgc                                                                                                         >  1:199693/1‑151 (MQ=255)

                                  gCCTGAAAGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGACGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGACCCGCGATACCGCCGTGCTGGAAGATGCCGGCGTACCGTATCAGCTGCTgg                                                                                                      <  1:191238/150‑1 (MQ=255)

                                   ccTGAAAGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGACGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGACCCGCGATACCGCCGTGCTGGAAGATGCCGGCGTACCGTATCAGCTGCTGGaa                                                                                                    <  1:484761/151‑1 (MQ=255)

                                            aTTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGACGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGACCCGCGATACCGCCGTGCTGGAAGATGCCGGCGTACCGTATCAGCTGCTGGAATCCAgccgc                                                                                           <  2:290153/151‑1 (MQ=255)

                                               gcgcgcCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGACGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGACCCGCGATACCGCCGTGCTGGAAGATGCCGGCGTACCGTATCAGCTGCTGGAATCCAGCCGCCt                                                                                         <  2:160308/150‑1 (MQ=255)

                                                      gAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGACGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGACCCGCGATACCGCCGTGCTGGAAGATGCCGGCGTACCGTATCAGCTGCTGGAATCCAGCCGCCTGGCGGa                                                                                   >  1:178954/1‑149 (MQ=255)

                                                           cAATATTCAGTATGAAGGGCGTCAGGGTGGGACGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGACCCGCGATACCGCCGTGCTGGAAGATGCCGGCGTACCGTATCAGCTGCTGGAATCCAGCCGCCTGGCGGAAGTGGAg                                                                            <  1:40846/151‑1 (MQ=255)

                                                            aaTATTCAGTATGAAGGGCGTCAGGGTGGGACGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGACCCGCGATACCGCCGTGCTGGAAGATGCCGGCGTACCGTATCAGCTGCTGGAATCCAGCCGCCTGGCGGAAGTGGAGc                                                                           <  1:473675/151‑1 (MQ=255)

                                                             atatTCAGTATGAAGGGCGTCAGGGTGGGACGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGACCCGCGATACCGCCGTGCTGGAAGATGCCGGCGTACCGTATCAGCTGCTGGAATCCAGCCGCCTGGCGGAAGTGGAGcc                                                                          >  2:79384/1‑151 (MQ=255)

                                                               atTCAGTATGAAGGGCGTCAGGGTGGGACGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGACCCGCGATACCGCCGTGCTGGAAGATGCCGGCGTACCGTATCAGCTGCTGGAATCCAGCCGCCTGGCGGAAGTGGAGccc                                                                         <  1:230731/150‑1 (MQ=255)

                                                                     tATGAAGGGCGTCAGGGTGGGACGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGACCCGCGATACCGCCGTGCTGGAAGATGCCGGCGTACCGTATCAGCTGCTGGAATCCAGCCGCCTGGCGGAAGTGGAGCCCGCGCTgg                                                                  >  1:568503/1‑151 (MQ=255)

                                                                      aTGAAGGGCGTCAGGGTGGGACGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGACCCGCGATACCGCCGTGCTGGAAGATGCCGGCGTACCGTATCAGCTGCTGGAATCCAGCCGCCTGGCGGAAGTGGAGCCCGCGCTGGc                                                                 >  1:789375/1‑151 (MQ=255)

                                                                              cGTCAGGGTGGGACGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGACCCGCGATACCGCCGTGCTGGAAGATGCCGGCGTACCGTATCAGCTGCTGGAATCCAGCCGCCTGGCGGAAGTGGAGCCCGCGCTGGCAGAAGTgg                                                         >  2:517385/1‑151 (MQ=255)

                                                                               gTCAGGGTGGGACGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGACCCGCGATACCGCCGTGCTGGAAGATGCCGGCGTACCGTATCAGCTGCTGGAATCCAGCCGCCTGGCGGAAGTGGAGCCCGCGCTGGCAGAAGTGgc                                                        >  1:840256/1‑151 (MQ=255)

                                                                                  aGGGTGGGACGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGACCCGCGATACCGCCGTGCTGGAAGATGCCGGCGTACCGTATCAGCTGCTGGAATCCAGCCGCCTGGCGGAAGTGGAGCCCGCGCTGGCAGAAGTGGCGca                                                     <  2:639244/151‑1 (MQ=255)

                                                                                   gggTGGGACGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGACCCGCGATACCGCCGTGCTGGAAGATGCCGGCGTACCGTATCAGCTGCTGGAATCCAGCCGCCTGGCGGAAGTGGAGCCCGCGCTGGCAGAAGTGGCGcac                                                    >  1:530462/1‑151 (MQ=255)

                                                                                   gggTGGGACGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGACCCGCGATACCGCCGTGCTGGAAGATGCCGGCGTACCGTATCAGCTGCTGGAATCCAGCCGCCTGGCGGAAGTGGAGCCCGCGCTGGCAGAAGTGGCGcac                                                    <  2:625925/151‑1 (MQ=255)

                                                                                    ggTGGGACGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGACCCGCGATACCGCCGTGCTGGAAGATGCCGGCGTACCGTATCAGCTGCTGGAATCCAGCCGCCTGGCGGAAGTGGAGCCCGCGCTGGCAGAAGTGGCGcaca                                                   <  1:847594/151‑1 (MQ=255)

                                                                                       gggACGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGACCCGCGATACCGCCGTGCTGGAAGATGCCGGCGTACCGTATCAGCTGCTGGAATCCAGCCGCCTGGCGGAAGTGGAGCCCGCGCTGGCAGAAGTGGCGCACAAAc                                                >  1:640583/1‑151 (MQ=255)

                                                                                                cAACTGTTCCGTACCGAACAACAGTATGAAAATGCGACCCGCGATACCGCCGTGCTGGAAGATGCCGGCGTACCGTATCAGCTGCTGGAATCCAGCCGCCTGGCGGAAGTGGAGCCCGCGCTGGCAGAAGTGGCGCACAAACTGACGggcg                                       <  2:85635/151‑1 (MQ=255)

                                                                                                  aCTGTTCCGTACCGAACAACAGTATGAAAATGCGACCCGCGATACCGCCGTGCTGGAAGATGCCGGCGTACCGTATCAGCTGCTGGAATCCAGCCGCCTGGCGGAAGTGGAGCCCGCGCTGGCAGAAGTGGCGCACAAACTGACGggcggc                                     <  1:158797/151‑1 (MQ=255)

                                                                                                       tCCGTACCGAACAACAGTATGAAAATGCGACCCGCGATACCGCCGTGCTGGAAGATGCCGGCGTACCGTATCAGCTGCTGGAATCCAGCCGCCTGGCGGAAGTGGAGCCCGCGCTGGCAGAAGTGGCGCACAAACTGACGGGCGGCCTGc                                 <  1:745751/150‑1 (MQ=255)

                                                                                                                   aacaGTATGAAAATGCGACCCGCGATACCGCCGTGCTGGAAGATGCCGGCGTACCGTATCAGCTGCTGGAATCCAGCCGCCTGGCGGAAGTGGAGCCCGCGCTGGCAGAAGTGGCGCACAAACTGACGGGCGGCCTGCAGTTACCCAatga                    <  1:81046/151‑1 (MQ=255)

                                                                                                                    acaGTATGAAAATGCGACCCGCGATACCGCCGTGCTGGAAGATGCCGGCGTACCGTATCAGCTGCTGGAATCCAGCCGCCTGGCGGAAGTGGAGCCCGCGCTGGCAGAAGTGGCGCACAAACTGACGGGCGGCCTGCAGTTACCCAatgat                   <  1:699397/151‑1 (MQ=255)

                                                                                                                         aTGAAAATGCGACCCGCGATACCGCCGTGCTGGAAGATGCCGGCGTACCGTATCAGCTGCTGGAATCCAGCCGCCTGGCGGAAGTGGAGCCCGCGCTGGCAGAAGTGGCGCACAAACTGACGGGCGGCCTGCAGTTACCCAATGATGAAAc              >  2:19163/1‑151 (MQ=255)

                                                                                                                           gAAAATGCGACCCGCGATACCGCCGTGCTGGAAGATGCCGGCGTACCGTATCAGCTGCTGGAATCCAGCCGCCTGGCGGAAGTGGAGCCCGCGCTGGCAGAAGTGGCGCACAAACTGACGGGCGGCCTGCAGTTACCCAATGATGAAACCg            <  1:92200/151‑1 (MQ=255)

                                                                                                                             aaaTGCGACCCGCGATACCGCCGTGCTGGAAGATGCCGGCGTACCGTATCAGCTGCTGGAATCCAGCCGCCTGGCGGAAGTGGAGCCCGCGCTGGCAGAAGTGGCGCACAAACTGACGGGCGGCCTGCAGTTACCCAATGATGAAACCgg           >  2:764322/1‑150 (MQ=255)

                                                                                                                              aaTGCGACCCGCGATACCGCCGTGCTGGAAGATGCCGGCGTACCGTATCAGCTGCTGGAATCCAGCCGCCTGGCGGAAGTGGAGCCCGCGCTGGCAGAAGTGGCGCACAAACTGACGGGCGGCCTGCAGTTACCCAATGATGAAACCgg           >  1:339642/1‑149 (MQ=255)

                                                                                                                                  cGACCCGCGATACCGCCGTGCTGGAAGATGCCGGCGTACCGTATCAGCTGCTGGAATCCAGCCGCCTGGCGGAAGTGGAGCCCGCGCTGGCAGAAGTGGCGCACAAACTGACGGGCGGCCTGCAGTTACCCAATGATGAAACCGGAGACTg     >  1:531578/1‑151 (MQ=255)

                                                                                                                                     cccGCGATACCGCCGTGCTGGAAGATGCCGGCGTACCGTATCAGCTGCTGGAATCCAGCCGCCTGGCGGAAGTGGAGCCCGCGCTGGCAGAAGTGGCGCACAAACTGACGGGCGGCCTGCAGTTACCCAATGATGAAACCGGAGACTGTCa  <  2:345219/151‑1 (MQ=255)

CGGATGGTGCGTCTGGCGGAATACAGCCGTGATTGCCTGAAAGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGACGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGACCCGCGATATCGCCGTGCTGGAAGATGCCGGCGTACCGTATCAGCTGCTGGAATCCAGCCGCCTGGCGGAAGTGGAGCCCGCGCTGGCAGAAGTGGCGCACAAACTGACGGGCGGCCTGCAGTTACCCAATGATGAAACCGGAGACTGTCA  >  NC_000913/1237889‑1238172

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A