Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A3 F50 I1 R1	300	54.7	1718999	98.4%	1691495	149.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,336,703	T→C	G291G (GGT→GGC)	acnA →	aconitate hydratase 1; aconitase A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,336,703	0	T	C	100.0%	187.0 / NA	51	G291G (GGT→GGC)	acnA	aconitate hydratase 1; aconitase A
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (22/29); total (22/29)

TTTCCATGCTTATCCCGGATGTAGTGGGCTTCAAACTTACCGGAAAATTACGTGAAGGTATTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTGATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAACCCTCGATTACATGCGTTTAAGCGGGCGCAGCGAAGATCAGGTCGAGTTGGT  >  NC_000913/1336555‑1336849

tttCCATGCTTATCCCGGATGTAGTGGGCTTCAAACTTACCGGAAAATTACGTGAAGGTATTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTGATGGCCt                                                                                                                                                  >  1:512557/1‑151 (MQ=255)

 ttCCATGCTTATCCCGGATGTAGTGGGCTTCAAACTTACCGGAAAATTACGTGAAGGTATTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTGATGGCCt                                                                                                                                                  <  1:342251/150‑1 (MQ=255)

  tCCATGCTTATCCCGGATGTAGTGGGCTTCAAACTTACCGGAAAATTACGTGAAGGTATTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTGATGGCCTgg                                                                                                                                                >  2:218487/1‑151 (MQ=255)

    cATGCTTATCCCGGATGTAGTGGGCTTCAACCTTACCGGAAAATTACGTGAAGGTATTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTGATGGCCTgg                                                                                                                                                <  1:667600/149‑1 (MQ=255)

         ttATCCCGGATGTAGTGGGCTTCAAACTTACCGGAAAATTACGTGAAGGTATTACCGCCACAGACCTGGGTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTGATGGCCTGGATTCACt                                                                                                                                         >  1:701178/1‑151 (MQ=255)

          tATCCCGGATGTAGTGGGCTTCAAACTTACCGGAAAATTACGTGAAGGTATTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTGATGGCCTGGATTCACt                                                                                                                                         >  2:77530/1‑150 (MQ=255)

             cccGGATGTAGTGGGCTTCAAACTTACCGGAAAATTACGTGAAGGTATTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTGATGGCCTGGATTCACTACCg                                                                                                                                     >  2:490979/1‑151 (MQ=255)

              ccGGATGTAGTGGGCTTCAAACTTACCGGAAAATTACGTGAAGGTATTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACACGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTGATGGCCTGGATTCACTACCGt                                                                                                                                    >  2:592529/1‑151 (MQ=255)

                  aTGTAGTGGGCTTCAAACTTACCGGAAAATTACGTGAAGGTATTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTGATGGCCTGGATTCACTACCGTTgg                                                                                                                                 <  2:72712/150‑1 (MQ=255)

                    gTAGTGGGCTTCAATCTGACCGGAAAATTACGTGAAGGTATTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTGATGGCCTGGATTCACTACCGTTGGc                                                                                                                                <  1:473172/149‑1 (MQ=255)

                          ggCTTCAAACTTACCGGAAAATTACGTGAAGGTATTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTGATGGCCTGGATTCACTACCGTTGGCGGATcgcg                                                                                                                        <  1:567773/151‑1 (MQ=255)

                                       ccGAAAAATTACGTGAAGGTATTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTGATGGCCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGcc                                                                                                             <  2:754088/149‑1 (MQ=255)

                                       ccGAAAAATTACGTGAAGGTATTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTGATGGCCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGcc                                                                                                             <  2:43687/149‑1 (MQ=255)

                                        cGGAAAATTACGTGAAGGTATTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTGATGGCCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAat                                                                                                          <  2:107256/151‑1 (MQ=255)

                                         ggAAAATTACGTGAAGGTATTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTGATGGCCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAat                                                                                                          >  1:771276/1‑150 (MQ=255)

                                               ttACGTGAAGGTATTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTGATGGCCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCg                                                                                                    <  1:44483/150‑1 (MQ=255)

                                                tACGTGAAGGTATTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTGATGGCCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGcc                                                                                                  >  1:97364/1‑151 (MQ=255)

                                                 aCGTGAAGCTATAACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACAGGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTGATGGCCTGGATTCACTACCGTTGGCGGATCGCGCCGCCATTGCCAATATGTCGcc                                                                                                  <  1:82347/150‑1 (MQ=255)

                                                       aGGTATTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTGATGGCCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAatat                                                                                           <  2:584449/151‑1 (MQ=255)

                                                       aGGCATTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTGATGGCCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAatat                                                                                           >  1:214965/1‑151 (MQ=255)

                                                        ggTATTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTGATGGCCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATg                                                                                          >  1:389020/1‑151 (MQ=255)

                                                          tATTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTGATGGCCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGt                                                                                        >  2:324627/1‑151 (MQ=255)

                                                           aTTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTGATGGCCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGTg                                                                                       <  2:96424/151‑1 (MQ=255)

                                                            ttACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTGATGGCCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGt                                                                                        <  1:770545/149‑1 (MQ=255)

                                                             tACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTGATGGCCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAatat                                                                                                        >  1:416160/1‑132 (MQ=255)

                                                             tACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTGATGGCCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAatat                                                                                                        <  2:416193/132‑1 (MQ=255)

                                                                  ccACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTGATGGCCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGTGCCACCtg                                                                                <  1:848899/151‑1 (MQ=255)

                                                                        aCCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTGATGGCCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGctt                                                                          <  1:450802/151‑1 (MQ=255)

                                                                           tGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTGATGGCCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGcttctt                                                                       >  2:331086/1‑151 (MQ=255)

                                                                              ttCTCAATGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTGATGGCCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCATCTTccc                                                                    >  1:821262/1‑151 (MQ=255)

                                                                                  cACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTGATGGCCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTCTTCCCAAt                                                                 <  2:195357/150‑1 (MQ=255)

                                                                                      gTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTGATGGCCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTCTTCCCAATCGa                                                              <  1:394544/149‑1 (MQ=255)

                                                                                         aCCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTGATGGCCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTCTTCCCAATCGATGCt                                                          >  1:43906/1‑150 (MQ=255)

                                                                                         aCCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTGATGGCCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTCTTCCCAATCGATGCTg                                                         <  1:166432/151‑1 (MQ=255)

                                                                                                 gctgcGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTGATGGCCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAACCCt                                                  <  1:46890/150‑1 (MQ=255)

                                                                                                 gctgcGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTGATGGCCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAACCCTc                                                 <  2:656317/151‑1 (MQ=255)

                                                                                                    gcgcAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTGATGGCCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAACCCTCGa                                               <  1:639499/150‑1 (MQ=255)

                                                                                                     cgcAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTGATGGCCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAACCCTCGAtt                                             <  1:405430/151‑1 (MQ=255)

                                                                                                              ggCGTGGTGGGGAAATTCGTCGAATTTTATGGTGATGGCCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAACCCTCGATTACATGCGtt                                    <  2:177028/151‑1 (MQ=255)

                                                                                                                      ggggAAATTCGTCGAATTTTATGGTGATGGCCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAACCCTCGATTACATGCGTTTAAGCggg                            >  1:8808/1‑151 (MQ=255)

                                                                                                                          aaaTTCGTCGAATTTTATGGTGATGGCCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAACCCTCGATTACATGCGTTTAAGCGGGCGCa                        <  2:674391/151‑1 (MQ=255)

                                                                                                                           aaTTCGTCGAATTTTATGGTGATGGCCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAACCCTCGATTACATGCGTTTAAGCGGgcgc                         >  1:159552/1‑149 (MQ=255)

                                                                                                                            aTTCGTCGAATTTTATGGTGATGGCCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAACCCTCGATTACATGCGTTTAAGCGGGCGCAGc                      >  2:811693/1‑151 (MQ=255)

                                                                                                                                gtcgAATTTTATGGTGATGGCCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAACCCTCGATTACATGCGTTTAAGCGGGCGCAGCGAAg                  <  1:295360/151‑1 (MQ=255)

                                                                                                                                  cgAATTTTATGGTGATGGCCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAACCCTCGATTACATGCGTTTAAGCGGGCGCAGCGa                    >  1:578041/1‑147 (MQ=255)

                                                                                                                                       tttCTGGTGATGGCCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGTCAATATGTCTCCAGTTTATGGTGCGACCTGTGGCTTCTTCCCAATCGATGCTGTAACCCTCGATTACATGCGTTTAAGCGGGCGCAGCGCAGATCAGGt           >  1:550341/1‑151 (MQ=255)

                                                                                                                                        ttATGGTGATGGCCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAACCCTCGATTACATGCGTTTAAGCGGGCGCAGCGAAGATCAGGTc          >  2:122167/1‑151 (MQ=255)

                                                                                                                                              tGATGGCCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAACCCTCGATTACATGCGTTTAAGCGGGCGCAGCGAAGATCAGGTCGAGTTg    >  1:278210/1‑151 (MQ=255)

                                                                                                                                               gATGGCCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAACCCTCGATTACATGCGTTTAAGCGGGCGCAGCGAAGATCAGGTCGAGTTg    <  2:295336/150‑1 (MQ=255)

                                                                                                                                                aTGGCCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAACCCTCGATTACATGCGTTTAAGCGGGCGCAGCGAAGATCAGGTCGAGTTGGt  <  2:46674/151‑1 (MQ=255)

                                                                                                                                                aTGGCCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAACCCTCGATTACATGCGTTTAAGCGGGCGCAGCGAAGATCAGGTCGAGTTGGt  <  2:404275/151‑1 (MQ=255)

TTTCCATGCTTATCCCGGATGTAGTGGGCTTCAAACTTACCGGAAAATTACGTGAAGGTATTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTGATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAACCCTCGATTACATGCGTTTAAGCGGGCGCAGCGAAGATCAGGTCGAGTTGGT  >  NC_000913/1336555‑1336849

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A