Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A3 F50 I1 R1	300	54.7	1718999	98.4%	1691495	149.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,351,196	(G)_7→8	intergenic (‑157/+211)	fabI ← / ← ycjD	enoyl‑[acyl‑carrier‑protein] reductase, NADH‑dependent/DUF559 family endonuclease‑related protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,351,189	1	.	G	91.7%	145.9 / 6.7	48	intergenic (‑150/+218)	fabI/ycjD	enoyl‑[acyl‑carrier‑protein] reductase, NADH‑dependent/DUF559 family endonuclease‑related protein
Reads supporting (aligned to +/- strand): ref base . (1/3); new base G (26/18); total (27/21)
Fisher's exact test for biased strand distribution p-value = 3.06e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01

GCTTTAATCCTTATTGTTGATGCTTGTTGTGCCTGAAAATCAGGCGATTCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCA‑‑GGG‑GGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGC‑AAATATTGTTACCCCAGCAACAAACAGGCTCAT‑ACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGTT  >  NC_000913/1351041‑1351324

gCTTTAATCCTTATTGTTGATGCTTGTTGTGCCTGAAAATCAGGCGATTCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCA‑‑gg                                                                                                                                          >  1:365079/1‑151 (MQ=255)

  tttAATCCTTATTGTTGATGCTTGTTGTGCCTGAAAATCAGGCGATTCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCA‑‑ggg‑g                                                                                                                                       >  2:309604/1‑151 (MQ=255)

           tATTGTTGATGCTTGTTGTGCCTGAAAATCAGGCGATTAGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAACAGGCTGCGGCA‑‑ggg‑gggggcgc                                                                                                                                >  1:309430/1‑145 (MQ=255)

            aTTGTTGATGCTTGTTGTGCCTGAAAATCAGGCGATTCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAG‑GGG‑GGGGCGCttt                                                                                                                              <  1:784476/151‑1 (MQ=255)

            aTTGTTGAGGCTTGTTGTGCCTGAAAATCAGGCGATTCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAG‑GGG‑GGGGCGCttt                                                                                                                              >  1:542191/1‑151 (MQ=255)

                  gATGCTTGTTGTGCCTGAAAATCAGGCGATTCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAG‑GGG‑GGGGCGCTTTTCCCCt                                                                                                                        >  2:250706/1‑151 (MQ=255)

                           tgtgCCTGAAAATCAGGCGATTCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAG‑GGG‑GGGGCGCTTTTCCCCTCAccc                                                                                                                   >  1:464279/1‑147 (MQ=255)

                              gCCTGAAAATCAGGCGATTCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAG‑GGG‑GGGGCGCTTTTCCCCTCACCCTAAccc                                                                                                             <  1:580923/150‑1 (MQ=255)

                                 tGAAAATCAGGCGATTCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAG‑GGG‑GGGGCGCTTTTCCCCTCCCCCTAACCCtctc                                                                                                         >  1:461829/1‑151 (MQ=255)

                                       tCAGGCGATTCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAG‑GGG‑GGGGCGCTTTTCCCCCCACCCTAACCCCCTaccc                                                                                                      >  2:507547/1‑148 (MQ=255)

                                           gCGATTCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAG‑GGG‑GGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAgggg                                                                                               <  1:741427/151‑1 (MQ=255)

                                            cGATTCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCAAGGTCCGACCGGAGCAGGCTGCGGCAG‑GGG‑GGGGCGCTTTTCCCCTCACCCTTAACCCCTTCCCCGAgggg                                                                                               >  2:731834/1‑150 (MQ=255)

                                               ttCGTCGTTTTAGTAAACAGTACGAACAGATCAACGGTTATTATATTCAACCTGGCTGTGAGTATTTATAGTTACTAGGTCCGCCCGGAGCAGGCTGCGGCC‑‑GGGAGGGGGGTTTTTCTCCTCCCCCTAAATCTCTCCCCaaag                                                                                                  >  1:558695/1‑144 (MQ=255)

                                                    cgtTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAG‑GGG‑GGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAgggg                                                                                        >  1:614031/1‑149 (MQ=255)

                                                     gtTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAG‑GGG‑GGGGCGCTTTTCCCCCCCCCCTCACCCCCCCCCCAGAGGGGGGAgggg                                                                                        >  2:696914/1‑148 (MQ=255)

                                                        ttAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAG‑GGG‑GGGGCGCTTTTTCCCTCACCCCAACCCTCTCCCCCGAGGGGTGAGGGGAcc                                                                                     >  2:729709/1‑148 (MQ=255)

                                                          aGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAG‑GGG‑GGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCCGAGGGGCGAGGGGACCGTAtt                                                                                >  1:26553/1‑151 (MQ=255)

                                                           gTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAG‑GGG‑GGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTAtt                                                                                >  1:339016/1‑150 (MQ=255)

                                                           gTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCA‑‑GGG‑GGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATtgt                                                                              <  2:328808/151‑1 (MQ=255)

                                                                  gTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAG‑GGG‑GGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGC‑AAat                                                                       <  1:588635/151‑1 (MQ=255)

                                                                    aCGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAG‑GGG‑GGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGC‑AAata                                                                      <  1:490444/150‑1 (MQ=255)

                                                                       aaCAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCA‑‑GGG‑GGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGC‑AAATATTGtt                                                                 <  2:134407/151‑1 (MQ=255)

                                                                          aGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAG‑GGG‑GGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGGCCGGATTGTGC‑AAATATTGtt                                                                 >  1:47221/1‑149 (MQ=255)

                                                                           gATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAG‑GGG‑GGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGC‑AAATATTGTTAcc                                                              <  2:461865/151‑1 (MQ=255)

                                                                              aaaCGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAG‑GGG‑GGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGC‑AAATATTGTTACCCCa                                                           <  2:47243/151‑1 (MQ=255)

                                                                              aaaCGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAG‑GGG‑GGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCCAGGGGGCCGCTTTGTGC‑AAATATTTTTAcccc                                                            >  1:442258/1‑150 (MQ=38)

                                                                                aCGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAG‑GGG‑GGGGCGCTTTTCCCCTCACCCTAACCCTTTCCCCAGAGGGGCGAGGGGACCGTATTGTGC‑AAATATTGTTACCCCAg                                                          >  2:248500/1‑150 (MQ=255)

                                                                                aCGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAG‑GGG‑GGGGCGCTTTTCCCCTCAACCTTACCCTCCCCCCAAGGGGGGGAGGGGGACGTATTGTGCAAAAAATTTTTAccc                                                             >  1:855557/1‑148 (MQ=255)

                                                                                     tattatAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAG‑GGG‑GGGGCGCTTTTCCCCTCACCCTAACCCCCTCCCCAGAGGGGGGAGGGGGCCGGATTTTGC‑AAATATTTTTTcccccacaaaa                                                     >  2:454861/1‑143 (MQ=255)

                                                                                      attatAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAG‑GGG‑GGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGC‑AAATATTGTTACCCCAGcaacaa                                                    <  2:154722/150‑1 (MQ=255)

                                                                                      attatAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGAAGCAGGCTGCGGCAG‑GGG‑GGGGCGCTTTTCCCCTCACCCCAACCCCCCCCCCAGAAGGGCGGGGGGGCCGTCTCGGGC‑AAATCTTTTTACCCCAGcaa                                                       >  1:781288/1‑147 (MQ=255)

                                                                                         ataATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAG‑GGG‑GGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGC‑AAATATTGTTACCCCAGCaacaaaca                                                 <  2:594644/150‑1 (MQ=255)

                                                                                           aaTCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAG‑GGG‑GGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGC‑AAATATTGTTACCCCAGCAACAAACAgg                                               <  2:84058/150‑1 (MQ=255)

                                                                                            aTCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAG‑GGG‑GGGGCGCTTTTCCCCTTCCCCTAACCCTCTCCCCCGAGGGGGGGGGGGCACGTATTTTGC‑AAATATTTTTAACCCCGCaacaaaaa                                                 >  1:433361/1‑145 (MQ=255)

                                                                                            aTCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAG‑GGG‑GGGGCGCTTTTCCCCTCACCCCAACCCTCCCCCCAGAGGGGCGAGGGGACCGTATTGTGC‑AAATATTGTTACCCCAGCAACAAACAgg                                               >  1:396290/1‑149 (MQ=255)

                                                                                               aaCCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAG‑GGG‑GGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGC‑AAATATTGTTACCCCAGCAACAAACAGGCTCAt                                          <  2:723457/151‑1 (MQ=255)

                                                                                                   tGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAG‑GGG‑GGGGCGCTTTTCCCCTCCCCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGC‑CAATATTGTTACCCCAGCAAAAAACAGGCTCAT‑ACa                                      >  2:105654/1‑150 (MQ=255)

                                                                                                      atgtgAGTAGCTTTAGTTGCGAGGTCCGACCGGAGCAGGCTGCATCAG‑GGG‑GGGGCGCTTTTCCCCTCCTCCTAACCCTTTCCCCACTGGGGGGGGGGGACCCTATTGTGC‑AAATATTTTTACCCCAGCAACAAAAAGGCTTAT‑ACAGcc                                   >  1:36056/2‑150 (MQ=255)

                                                                                                        gtgAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAG‑GGG‑GGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGC‑AAATATTGTTACCCCAGCAACAAACAGGCTCAT‑ACAGCCCCt                                <  1:154666/151‑1 (MQ=255)

                                                                                                          gAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAG‑GGG‑GGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCCGAGGGGCGGGGGGACCGTATTGTGC‑CAATATTTTTACCCCCGCAACAAACAGGCTCAT‑ACAGcccc                                 >  2:686571/1‑148 (MQ=38)

                                                                                                          gAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAG‑GGG‑GGGGCGCTTTTCCCCTCACCCTAAACCTCTCCCCCGAGGGGCGGGGGGGCCGGATTGTGC‑AAATATTGTTAACCCAGCAACAAACAGGCTCAT‑ACAGccccca                               >  2:837666/1‑148 (MQ=255)

                                                                                                           aGTAGCTATAGTAGCCAGGTCTGACCGGAGCAGGCTGTGGCA‑‑GGG‑GGGGCTCTTTTCTCCTCACCCTAACCCTCTCCCCAGACGGGCGAGGGGACCGTATTGTGT‑AAATATTGTTACCCCAGCAACAAACAGGCTCAT‑ACAGCGCCTaa                              <  1:344357/149‑1 (MQ=38)

                                                                                                             tAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAG‑GGG‑GGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGGGGGGACCGTATTGTGC‑CAATATTGTTACCCCCGCAAAAAACAGGCTCAT‑ACAACCCCTAAccc                           >  2:90300/1‑151 (MQ=38)

                                                                                                               gCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAG‑GGG‑GGGGCGCTTTTCCCCTCACCCTTAACCTCTCCCCCGAGGGGCGGGGGGACCGGATTGTGC‑AAATATTTTTACCCCCGCAACAAACAAGGTCATAACGGCCCCAAAcctt                          >  2:148244/1‑149 (MQ=255)

                                                                                                                  ataGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGG‑GGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGC‑AAATATTGTTACCCCAGCAACAAACAGGCTCAT‑ACAGCCCCTAACCCtt                         <  2:485146/149‑1 (MQ=255)

                                                                                                                    aGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAG‑GGG‑GGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGC‑AAATATTGTTACCCCAGCAACAAACAGGCTCAT‑ACAGCCCCTAACCCTTTCATg                    <  2:570053/151‑1 (MQ=255)

                                                                                                                         ccAGGTCCGACCGGAGCAGGCTGCGGCAG‑GGG‑GGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCCGAGGGGCGAGGGGGCCGTATTGTGC‑AAATATTGTTACCCCAGCAACAAACAGGCTCAT‑ACAGCCCCTAACCCTTTCATGGCGa                >  1:388322/1‑150 (MQ=255)

                                                                                                                           aGGTCCGACCGGAGCAGGCTGCGGCAG‑GGG‑GGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGC‑AAATATTGTTACCCCAGCAACAAACAGGCTCAT‑ACAGCCCCTAACCCTTTCATGGCGATgg             <  1:300063/151‑1 (MQ=255)

                                                                                                                                cGACCGGAGCAGGCTGCGGCAG‑GGG‑GGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGC‑AAATATTGTTACCCCAGCAACAAACAGGCTCAT‑ACAGCCCCTAACCCTTTCATGGCGATGGCTggg        <  1:681319/151‑1 (MQ=255)

                                                                                                                                     ggAGCAGGCTGCGGCAG‑GGG‑GGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGC‑AAATATTGTTACCCCAGCAACAAACAGGCTCAT‑ACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGt   <  1:674120/151‑1 (MQ=255)

                                                                                                                                      gAGCAGGCTGCGGCAG‑GGG‑GGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGC‑AAATATTGTTACCCCAGCAACAAACAGGCTCAT‑ACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGtt  <  2:725459/151‑1 (MQ=255)

GCTTTAATCCTTATTGTTGATGCTTGTTGTGCCTGAAAATCAGGCGATTCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCA‑‑GGG‑GGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGC‑AAATATTGTTACCCCAGCAACAAACAGGCTCAT‑ACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGTT  >  NC_000913/1351041‑1351324

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A