Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A3 F50 I1 R1 300 54.7 1718999 98.4% 1691495 149.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,381,934 A→G intergenic (+32/‑13) ycjV → / → ompG pseudogene; putative ATP‑binding component of a transport system/outer membrane porin G

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,381,9340AG100.0% 210.7 / NA 57intergenic (+32/‑13)ycjV/ompGpseudogene; putative ATP‑binding component of a transport system/outer membrane porin G
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (28/29);  total (28/29)

TAGTGGTCCGTGCTGGTGCGTTAAATGATTATCATGCAGGAGAAAATATCACTATTCATTTTGATATGACGAAATGTCATTTCTTTGATGCAGAAACGGAAATAGCAATTCGCTAAATACAGGGGGAAGGCATTCCCCCAGGATAATACAAGGAACAATAATGAAAAAGTTATTACCCTGTACCGCACTGGTGATGTGTGCGGGAATGGCCTGCGCACAGGCCGAGGAAAGGAACGACTGGCACTTTAATATCGGCGCGATGTACGAAATAGAAAACGTCGAGGGTTATGGCG  >  NC_000913/1381787‑1382079
                                                                                                                                                   |                                                                                                                                                 
tAGTGGTCCGTGCTGGTGCGTTAAATGATTATCATGCAGGAGAAAATATCACTATTCATTTTGATATGACGAAATGTCATTTCTTTGATGCAGAAACGGAAATAGCAATTCGCTAAATACAGGGGGAAGGCATTCCCCCAGGATAATGCa                                                                                                                                                 >  2:107894/1‑150 (MQ=255)
         gTGCTGGTGCGTTAAATGATTATCATGCAGGAGAAAATATCACTATTCATTTTGATATGACGAAATGTCATTTCTTTGATGCAGAAACGGAAATAGCAATTCGCTAAATACAGGGCGAAGGCATTCCCCCATGATAATGCAAGGAACaata                                                                                                                                       <  2:703087/151‑1 (MQ=255)
           gCTGGTGCGTTAAATGATTATCATGCAGGAGAAAATATCACTATTCATTTTGATATGACGAAATGTCATTTCTTTGATGCAGAAACGGAAATAGCAATTCGCTAAATACAGGGGGAAGGCATTCCCCCAGGATAATGCAAGGAACaataat                                                                                                                                     >  1:152842/1‑151 (MQ=255)
           gCTGGTGCGTTAAATGATTATCATGCAGGAGAAAATATCACTATTCATTTTGATATGACGAAATGTCATTTCTTTGATGCAGAAACGGAAATAGCAATTCGCTAAATACAGGGGGAAGGCATTCCCCCAGGATAATGCAAGGAACaataat                                                                                                                                     <  1:525216/151‑1 (MQ=255)
           gCTGGTGCGTTAAATGATTATCATGCAGGAGAAAATATCACTATTCATTTTGATATGACGAAATGTCATTTCTTTGATGCAGAAACGGAAATAGCAATTCGCTAAATACAGGGGGAAGGCATTCCCCCAGGATAATGCAAGGAACaataa                                                                                                                                      <  1:156761/150‑1 (MQ=255)
             tGGTGCGTTAAATGATTATCATGCAGGAGAAAATATCACTATTCATTTTGATATGACGAAATGTCATTTCTTTGATGCAGAAACGGAAATAGCAATTCGCTAAATACAGGGGGAAGGCATTCCCCCAGGATAATGCAAGGAACAATAATg                                                                                                                                    >  1:623583/1‑150 (MQ=255)
              ggTGCGTTAAATGATTATCATGCAGGAGAAAATATCACTATTCATTTTGATATGACGAAATGTCATTTCTTTGATGCAGAAACGGAAATAGCAATTCGCTAAATACAGGGGGAAGGCATTCCCCCAGGATAATGCAAGGAACAATAATg                                                                                                                                    <  1:304405/149‑1 (MQ=255)
                tGCGTTAAATGATTATCATGCAGGAGAAAATATCACTATTCATTTTGATATGACGAAATGTCATTTCTTTGATGCAGAAACGGAAATAGCAATTCGCTAAATACAGGGGGAAGGCATTCCCCCAGGATAATGCAAGGAACAATAATGaaa                                                                                                                                 >  1:446527/1‑150 (MQ=255)
                 gCGTTAAATGATTATCATGCAGGAGAAAATATCACTATTCATTTTGATATGACGAAATGTCATTTCTTTGATGCAGAAACGGAAATAGCAATTCGCTAAATACAGGGGGAAGGCATTCCCCCAGGATAATGCAAGGAACAATAATGaaaaa                                                                                                                               <  1:532359/151‑1 (MQ=255)
                  cGTTAAATGATTATCATGCAGGAGAAAATATCACTATTCATTTTGATATGACGAAATGTCATTTCTTTGATGCAGAAACGGAAATAGCAATTCGCTAAATACAGGGGGAAGGCATTCCCCCAGGATAATGCAAGGAACAATAATGaaaa                                                                                                                                >  2:804244/1‑149 (MQ=255)
                         tGATTACCATGCAGGAGAAAATATCACTATTCATTTTGATATGACGAAATGTCATTTCTTTGATGCAGAAACGGAAATAGCAATTCGCTAAATACAGGGGGAAGGCATTCCCCCAGGATAATGCAAGGAACAATAATGAAAAAGTTATTAc                                                                                                                       >  2:858043/1‑151 (MQ=255)
                          gATTATCATGCAGGAGAAAATATCACTATTCATTTTGATATGACGAAATGTCATTTCTTTGATGCAGAAACGGAAATAGCAATTCGCTAAATACAGGGGGAAGGCATTCCCCCAGGATAATGCAAGGAACAATAATGAAAAAGTTATTAc                                                                                                                       <  2:61858/150‑1 (MQ=255)
                          gATTATCATGCAGGAGAAAATATCACTATTCATTTTGATATGACGAAATGTCATTTCTTTGATGCAGAAACGGAAATAGCAATTCGCTAAATACAGGGGGAAGGCATTCCCCCAGGATAATGCAAGGAACAATAATGAAAAAGTTATTAc                                                                                                                       >  1:800933/1‑150 (MQ=255)
                           aTTATCATGCAGGAGAAAATATCACTATTCATTTTGATATGACGAAATGTCATTTCTTTGATGCAGAAACGGAAATAGCAATTCGCTAAATACAGGGGGAAGGCATTCCCCCAGGATAATGCAAGGAACAATAATGAAAAAGTTATTAccc                                                                                                                     >  2:459717/1‑151 (MQ=255)
                               tCATGCAGGAGAAAATATCACTATTCATTTTGATATGACGAAATGTCATTTCTTTGATGAAGAAACGGAAATAGCAATTCGCTAAATACAGGGGGAAGGCATTCCCCCAGGATAATGCAAGGAACAATAATGAAAAAGTTATTACCCTGt                                                                                                                  >  2:770609/1‑150 (MQ=255)
                                  tGCAGGAGAAAATATCACTATTCATTTTGATATGACGAAATGTCATTTCTTTGATGCAGAAACGGAAATAGCAATTCGCTAAATACAGGGGGAAGGCATTCCCCCAGGATAATGCAAGGAACAATAATGAAAAAGTTATTACCCTGTACCg                                                                                                              >  2:31609/1‑151 (MQ=255)
                                     aGGAGAAAATATCACTATTCATTTTGATATGACGAAATGTCATTTCTTTGATGCAGAAACGGAAATAGCAATTCGCTAAATACAGGGGGAAGGCATTCCCCCAGGATAATGCAAGGAACAATAATGAAAAAGTTATTACCCTGTACCGCAc                                                                                                           >  1:274729/1‑151 (MQ=255)
                                      ggggaAAATATCACTATTCATTTTGATATGAAGAAATGTCATTTCGTTAATGCAGAAACGGAAATAGCAATTCGCTAAATACAGGGGGAAGGCATTACCCCAGGATAATGCAAGGAACAATAATGAAAAAGTTATTACCATGTACCGCACt                                                                                                          >  1:209964/4‑151 (MQ=255)
                                      ggAGAAAATATCACTATTCATTTTGATATGACGAAATGTCATTTCTTTGATGCAGAAACGGAAATAGCAATTCGCTAAATACAGGGGGAAGGCATTCCCCCAGGATAATGCAAGGAACAATAATGAAAAAGTTATTACCCTGTACCGCACt                                                                                                          <  1:119323/151‑1 (MQ=255)
                                       gagaAAATATCACTATTCATTTTGATATGACGAAATGTCATTTCTTTGATGCAGAAACGGAAATAGCAATTCGCTAAATACAGGGGGAAGGCATTCCCCCAGGATAATGCAAGGAACAATAATGAAAAAGTTATTACCCTGTACCGCACTg                                                                                                         <  1:230991/151‑1 (MQ=255)
                                         gaAAATATCACTATTCATTTTGATATGACGAAATGTCATTTCTTTGATGCAGAAACGGAAATAGCAATTCGCTAAATACAGGGGGAAGGCATTCCCCCAGGATAATGCAAGGAACAATAATGAAAAAGTTATTACCCTGTACCGCACTGGt                                                                                                       <  2:413382/151‑1 (MQ=255)
                                              tatCACTATTCATTTTGATATGACGAAATGTCATTTCTTTGATGCAGAAACGGAAATAGCAATTCGCTAAATACAGGGGGAAGGCATTCCCCCAGGATAATGCAAGGAACAATAATGAAAAAGTTATTACCCTGTACCGCACTGGTGAtgt                                                                                                  >  2:185844/1‑151 (MQ=255)
                                               atCACTATTCATTTTGATATGACGAAATGTCATTTCTTTGATGCAGAAACGGAAATAGCAATTCGCTAAATACAGGGGGAAGGCATTCCCCCAGGATAATGCAAGGAACAATAATGAAAAAGTTATTACCCTGTACCGCACTGGTGAtgtg                                                                                                 <  2:429555/151‑1 (MQ=255)
                                                  aCTATTCATTTTGATATGACGAAATGTCATTTCTTTGATGCAGAAACGGAAATAGCAATTCGCTAAATACAGGGGGAAGGCATTCCCCC‑GGATAATGCAAGGAACAATAATGAAAAAGTTATTACCCTGTACCGCACTGGTGATGTGTGCg                                                                                             >  1:369151/1‑151 (MQ=255)
                                                     aTTCATTTTGATATGACGAAATGTCATTTCTTTGATGCAGAAACGGAAATAGCAATTCGCTAAATACAGGGGGAAGGCATTCCCCCAGGATAATGCAAGGAACAATAATGAAAAAGTTATTACCCTGTACCGCACTGGTGATGTGTGCggg                                                                                           >  2:94080/1‑151 (MQ=255)
                                                     aTTCATTTTGATATGACGAAATGTCATTTCTTTGATGCAGAAACGGAAATAGCAATTCGCTAAATACAGGGGGAAGGCATTCCCCCAGGATAATGCAAGGAACAATAATGAAAAAGTTATTACCCTGTACCGCACTGGTGATGTGTGCggg                                                                                           >  1:345802/1‑151 (MQ=255)
                                                     aTTCATTTTGATATGACGAAATGTCATTTCTTTGATGCAGAAACGGAAATAGCAATTCGCTAAATACAGGGGGAAGGCATTCCCCCAGGATAATGCAAGGAACAATAATGAAAAAGTTATTACCCTGTACCGCACTGGTGATGTGTGCgg                                                                                            <  2:430785/150‑1 (MQ=255)
                                                     aTTCATTTTGATATGACGAAATGTCATTTCTTTGATGCAGAAACGGAAATAGCAATTCGCTAAATACAGGGGGAAGGCATTCCCCCAGGATAATGCAAGGAACAATAATGAAAAAGTTATTACCCTGTACCGCACTGGTGATGTGTGCgg                                                                                            <  2:748015/150‑1 (MQ=255)
                                                      ttCATTTTGATATGACGAAATGTCATTTCTTTGATGCAGAAACGGAAATAGCAATTCGCTAAATACAGGGGGAAGGCATTCCCCCAGGATAATGCAAGGAACAATAATGAAAAAGTTATTACCCTGTACCGCACTGGTGATGTGTGCggg                                                                                           >  2:8297/1‑150 (MQ=255)
                                                              gATATGACGAAATGTCATTTCTTTGATGCAGAAACGGAAATAGCAATTCGCTAAATACAGGGGGAAGGCATTCCCCCAGGATAATGCAAGGAACAATAACGAAAAAGTTATTACCCTGTACCGCACTGGTGATGTGTGCGGGAATGGCCTg                                                                                  <  2:51346/151‑1 (MQ=255)
                                                                tatGACGAAATGTCATTTCTTTGATGCAGAAACGGAAATAGCAATTCGCTAAATACAGGGGGAAGGCATTCCCCCAGGATAATGCAAGGAACAATAATGAAAAAGTTATTACCCTGTACCGCACTGGTGATGTGTGCGGGAATGGCCTgcg                                                                                >  2:664093/1‑151 (MQ=255)
                                                                 atGACGAAATGTCATTTCTTTGATGCAGAAACGGAAATAGCAATTCGCTAAATACAGGGGGAAGGCATTCCCCCAGGATAATGCAAGGAACAATAATGAAAAAGTTATTACCCTGTACCGCACTGGTGATGTGTGCGGGAATGGCCTgcgc                                                                               <  1:323164/151‑1 (MQ=255)
                                                                        aaTGTCATTTCTTTGATGCAGAAACGGAAATAGCAATTCGCTAAATACAGGGGGAAGGCATTCCCCCAGGATAATGCAAGGAACAATAATGAAAAAGTTATTACCCTGTACCGCACTGGTGATGTGTGCGGGAATGGCCTGCGCACAGGcc                                                                        >  2:198736/1‑151 (MQ=255)
                                                                                        tGCAGAAACGGAAATAGCAATTCGCTAAATACAGGGGGAAGGCATTCCCCCAGGATAATGCAAGGAACAATAATGAAAAAGTTATTACCCTGTACCGCACTGGTGATGTGTGCGGGAATGGCCTGCGCACAGGCCGAGGAAAGGAACGACt                                                        >  1:649463/1‑151 (MQ=255)
                                                                                           aGAAACGGAAATAGCAATTCGTTAAATACAGGGGGAAGGCATTCCCTCAGGATAATGCAAGGAACAATAATGAAAAAGTTATTACCCTGTACCGCACTGGTGATGTGTGCGGGAATGGCCTGCGCAGAGGCTGAGGGAAGGAACGACTg                                                       >  1:737724/1‑149 (MQ=255)
                                                                                           aGAAACGGAAATAGCAATTCGCTAAATACAGGGGGAAGGCATTCCCCCAGGATAATGCAAGGAACAATAATGAAAAAGTTATTACCCTGTACCGCACTGGTGATGTGTGCGGGAATGGCCTGCGCACAGGCCGAGGAAAGGAACGACTGGc                                                     <  1:80360/151‑1 (MQ=255)
                                                                                            gAAACGGAAATAGCAATTCGCTAAATACAGGGGGAAGGCATTCCCCCAGGATAATGCAAGGAACAATAATGAAAAAGTTATTACCCTGTACCGCACTGGTGATGTGTGCgg                                                                                            >  2:808171/1‑111 (MQ=255)
                                                                                            gAAACGGAAATAGCAATTCGCTAAATACAGGGGGAAGGCATTCCCCCAGGATAATGCAAGGAACAATAATGAAAAAGTTATTACCCTGTACCGCACTGGTGATGTGTGCgg                                                                                            <  1:808115/111‑1 (MQ=255)
                                                                                             aaaCGGAAATAGCAATTCGCTAAATACAGGGGGAAGGCATTCCCCCAGGATAATGCAAGGAACAATAATGAAAAAGTTATTACCCTGTACCGCACTGGTGATGTGTGCGGGAATGGCCTGCGCACAGGCCGAGGAAAGGAACGACTGGCAc                                                   <  2:544474/151‑1 (MQ=255)
                                                                                                 ggAAATAGCAATTCGCTAAATACAGGGGGAAGGCATTCCCCCAGGATAATGCAAGGAACAATAATGAAAAAGTTATTACCCTGTACCGCACTGGTGATGTGTGCGGGAATGGCCTGCGCACAGGCCGAGGAAAGGAACGACTGGCACTTTa                                               <  1:765558/151‑1 (MQ=255)
                                                                                                   aaaTAGCAATTCGCTAAATACAGGGGGAAGGCATTCCCCCAGGATAATGCAAGGAACAATAATGAAAAAGTTATTACCCTGTACCGCACTGGTGATGTGTGCGGGAATGGCCTGCGCACAGGCCGAGGAAAGGAACGACTGGCACTTTAat                                             <  1:31587/151‑1 (MQ=255)
                                                                                                   aaaTAGCAATTCGCTAAATACAGGGGGAAGGCATTCCCCCAGGATAATGCAAGGAACAATAATGAAAAAGTTATTACCCTGTACCGCACTGGTGATGTGTGCGGGAATGGCCTGCGCACAGGCCGAGGAAAGGAACGACTGGCACTTTAat                                             <  2:169990/151‑1 (MQ=255)
                                                                                                   aaaTAGCAATTCGCTAAATACAGGGGGAAGGCATTCCCCCAGGATAATGCAAGGAACAATAATGAAAAAGTTATTACCCTGTACCGCACTGGTGATGTGTGCGGGAATGGCCTGCGCACAGGCCGAGGAAAGGAACGACTGGCACTTTAat                                             >  1:13135/1‑151 (MQ=255)
                                                                                                                        aGGGGGAAGGCATTCCCCCAGGATAATGCAAGGAACAATAATGAAAAAGTTATTACCCTGTACCGCACTGGTGATGTGTGCGGGAATGGCCTGCGCACAGGCCGAGGAAAGGAACGACTGGCACTTTAATATCGGCGCGATGTACGAAATa                        <  1:806944/151‑1 (MQ=255)
                                                                                                                            ggAAGGCATTCCCCCAGGATAATGCAAGGAACAATAATGAAAAAGTTATTACCCTGTACCGCACTGGTGATGTGTGCGGGAATGGCCTGCGCACAGGCCGAGGAAAGGAACGACTGGCACTTTAATATCGGCGCGATGTACGAAATAGaaa                    <  2:345825/151‑1 (MQ=255)
                                                                                                                            ggAAGGCATTCCCCCAGGATAATGCAAGGAACAATAATGAAAAAGTTATTACCCTGTACCGCACTGGTGATGTGTGCGGGAATGGCCTGCGCACAGGCCGAGGAAAGGAACGACTGGCACTTTAATATCGGCGCGATGTACGAAATAGaaa                    <  1:105327/151‑1 (MQ=255)
                                                                                                                             gAAGGCATTCCCCCAGGATAATGCAAGGAACAATAATGAAAAAGTTATTACCCTGTACCGCACTGGTGATGTGTGCGGGAATGGCCTGCGCACAGGCCGAGGAAAGGAACGACTGGCACTTTAATATCGGCGCGATGTACGAAATAGaaaa                   <  2:346861/151‑1 (MQ=255)
                                                                                                                              aaGGCATTCCCCCAGGATAATGCAAGGAACAATAATGAAAAAGTTATTACCCTGTACCGCACTGGTGATGTGTGCGGTAATGGCCTGCGCACAGGCCGAGGAAAGGAACGACTGGCACTTTAATATCGGCGCGATGTACGAAATAGAAAAc                  <  1:390633/151‑1 (MQ=255)
                                                                                                                                 gCATTCCCCCAGGATAATGCAAGGAACAATAATGAAAAAGTTATTACCCTGTACCGCACTGGTGATGTGTGCGGGAATGGCCTGCGCACAGGCCGAGGAAAGGAACGACTGGCACTTTAATATCGGCGCGATGTACGAAATAGAAAACGTc               >  2:582903/1‑151 (MQ=255)
                                                                                                                                 gCATTCCCCCAGGATAATGCAAGGAACAATAATGAAAAAGTTATTACCCTGTACCGCACTGGTGATGTGTGCGGGAATGGCCTGCGCACAGGCCGAGGAAAGGAACGACTGGCACTTTAATATCGGCGCGATGTACGAAATAGAAAACGTc               <  1:783676/151‑1 (MQ=255)
                                                                                                                                  cATTCCCCCAGGATAATGCAAGGAACAATAATGAAAAAGTTATTACCCTGTACCGCACTGGTGATGTGTGCGGGAATGGCCTGCGCACAGGCCGAGGAAAGGAACGACTGGCACTTTAATATCGGCGCGATGTACGAAATAGAAAACGTCg              >  2:234968/1‑151 (MQ=255)
                                                                                                                                     tCCCCCAGGATAATGCAAGGAACAATAATGAAAAAGTTATTACCCTGTACCGCACTGGTGATGTGTGCGGGAATGGCCTGCGCACAGGCCGAGGAAAGGAACGACTGGCACTTTAATATCGGCGCGATGTACGAAATAGAAAACGTCGAg            <  2:133090/150‑1 (MQ=255)
                                                                                                                                      cccccAGGATAATGCAAGGAACAATAATGAAAAAGTTATTACCCTGTACCGCACTGGTGATGTGTGCGGGAATGGCCTGCGCACAGGCCGAGGAAAGGAACGACTGGCACTTTAATATCGGCGCGATGTACGAAATAGAAAACGTCGAggg          >  1:493580/1‑151 (MQ=255)
                                                                                                                                        cccAGGATAATGCAAGGAACAATAATGAAAAAGTTATTACCCTGTACCGCACTGGTGATGTGTGCGGGAATGGCCTGCGCACAGGCCGAGGAAAGGAACGACTGGCACTTTAATATCGGCGCGATGTACGAAATAGAAAACGTCGAGGGtt        >  1:601015/1‑151 (MQ=255)
                                                                                                                                          cAGGATAATGCAAGGAACAATAATGAAAAAGTTATTACCCTGTACCGCACTGGTGATGTGTGCGGGAATGGCCTGCGCACAGGCCGAGGAAAGGAACGACTGGCACTTTAATATCGGCGCGATGTACGAAATAGAAAACGTCGAGGGTTAt      <  2:736011/151‑1 (MQ=255)
                                                                                                                                          cAGGATAATGCAAGGAACAATAATGAAAAAGTTATTACCCTGTACCGCACTGGTGATGTGTGCGGGAATGGCCTGCGCACAGGCCGAGGAAAGGAACGACTGGCACTTTAATATCGGCGCGATGTACGAAACAGAAAACGTCGAGGGTTAt      <  2:708419/151‑1 (MQ=255)
                                                                                                                                             gATAATGCAAGGAACAATAATGAAAAAGTTATTACCCTGTACCGCACTGGTGATGTGTGCGGGAATGGCCTGCGCACAGGCCGAGGAAAGGAACGACTGGCACTTTAATATCGGCGCGATGTACGAAATAGAAAACGTCGAGGGTTATGGc   >  2:393272/1‑151 (MQ=255)
                                                                                                                                              ataatgCAAGGAACAATAATGAAAAAGTTATTACCCTGTACCGCACTGGTGATGTGTGCGGGAATGGCCTGCGCACAGGCCGAGGAAAGGAACGACTGGCACTTTAATATCGGCGCGATGTACGAAATAGAAAACGTCGAGGGTTATGGCg  <  2:419185/151‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                 
TAGTGGTCCGTGCTGGTGCGTTAAATGATTATCATGCAGGAGAAAATATCACTATTCATTTTGATATGACGAAATGTCATTTCTTTGATGCAGAAACGGAAATAGCAATTCGCTAAATACAGGGGGAAGGCATTCCCCCAGGATAATACAAGGAACAATAATGAAAAAGTTATTACCCTGTACCGCACTGGTGATGTGTGCGGGAATGGCCTGCGCACAGGCCGAGGAAAGGAACGACTGGCACTTTAATATCGGCGCGATGTACGAAATAGAAAACGTCGAGGGTTATGGCG  >  NC_000913/1381787‑1382079

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A