Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A3 F50 I1 R1	300	54.7	1718999	98.4%	1691495	149.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,642,084	G→A	A254V (GCG→GTG)	rodZ ←	MreB assembly cytoskeletal protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,642,084	0	G	A	97.9%	161.6 / ‑4.3	47	A254V (GCG→GTG)	rodZ	MreB assembly cytoskeletal protein
Reads supporting (aligned to +/- strand): ref base G (0/1); new base A (23/23); total (23/24)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.67e-01

GGCGCACCAATTTTCAGTTTGTACGGTGCCTGGCCGGTTAAGTTCAAATTACCGTCTTTACGCTGCATACCGCTAAACAATTTTTTACCGGTAGCATCAGTGACCTCCAGCCAGCAATCGGCAGTAAAGTTCATCACCAGCGCATTCGGATCAGCCACCGGCGTGGTCACGCCAGCCTGATCGGTTGGCAACGGCGCAGCACCATCTGGCGTTGTTGCTGCCGTTGGTGCCGGGGTCGCGGCGGTATCAACATTTGCCTGCGAAGGCGAAACAACCGCATTCTGTTGCG  >  NC_000913/2641943‑2642231

ggCGCACCAATTTTCAGTTTGTACGGTGCCTGGCCGGTTAAGTTCAAATTACCGTCTTTACGCTGCATACCGCTAAACAATTTTTTACCGGTAGCATCAGTGACCTCCAGCCAGCAATCGGCAGTAAAGTTCATCACCAGCACATTCGGAt                                                                                                                                            >  1:479699/1‑151 (MQ=255)

          ttttCAGTTTGCACGGTGCCTGGCCGGTTAAGTTCAAATTACCGTCTTTACGCTGCATACCGCTAAACAATTTTTTACCGGTAGCATCAGTGACCTCCAGCCAGCAATCGGCAGTAAAGTTCATCACCTGCACTTTCGGATCAGCCACCg                                                                                                                                   <  1:653041/150‑1 (MQ=255)

                       cGGTGCCTGGCCGGTTAAGTTCAAATTACCGTCTTTACGCTGCATACCGCTAAACAATTTTTTACCGGTAGCATCAGTGACCTCCAGCCAGCAATCGGCAGTAAAGTTCATCACCAGCACATTCGGATCAGCCACCGGCGTGGTCACGCCa                                                                                                                     <  2:410841/151‑1 (MQ=255)

                              tGGCCGGTTAAGTTCAAATTACCGTCTTTACGCTGCATACCGCTAAACAATTTTTTACCGGTAGCATCAGTGACCTCCAGCCAGCAATCGGCAGTAAAGTTCATCACCAGCACATTCGGATCAGCCACCGGCGTGGTCACGCCAGCCTGAt                                                                                                              >  2:524537/1‑151 (MQ=255)

                                  cGGTTAAGTTCAAATTACCGTCTTTACGCTGCATACCGCTAAACAATTTTTTACCGGTAGCATCAGTGACCTCCAGCCAGCAATCGGCAGTAAAGTTCATCACCAGCACATTCGGATCAGCCACCGGCGTGGTCACGCCAGCCTGATCgg                                                                                                           >  2:466994/1‑150 (MQ=255)

                                  cGGTTAAGTTCAAATTACCGTCTTTACGCTGCATACCGCTAAACAATTTTTTACCGGTAGCATCAGTGACCTCCAGCCAGCAATCGGCAGTAAAGTTCATCACCAGCACATTCGGATCAGCCACCGGCGTGGTCACGCCAGCCTGATCGGt                                                                                                          >  2:135021/1‑151 (MQ=255)

                                   ggTTAAGTTCAAATTACCGTCTTTACGCTGCATACCGCTAAACAATTTTTTACCGGTAGCATCAGTGACCTCCAGCCAGCAATCGGCAGTAAAGTTCATCACCAGCACATTCGGATCAGCCACCGGCGTGGTCACGCCAGCCTGATCGGtt                                                                                                         >  1:182134/1‑151 (MQ=255)

                                    gTTAAGTTCAAATTACCGTCTTTACGCTGCATACCGCTAAACAATTTTTTACCGGTAGCATCAGTGACCTCCAGCCAGCAATCGGCAGTAAAGTTCATCACCAGCACATTCGGATCAGCCACCGGCGTGGTCACGCCAGCCTGATCGGTTg                                                                                                        <  1:283246/151‑1 (MQ=255)

                                    gTTAAGTTCAAATTACCGTCTTTACGCTGCATACCGCTAAACAATTTTTTACCGGTAGCATCAGTGACCTCCAGCCAGCAATCGGCAGTAAAGTTCATCACCAGCACATTCGGATCAGCCACCGGCGTGGTCACGCCAGCCTGATCGGTTg                                                                                                        <  1:4187/151‑1 (MQ=255)

                                    gTTAAGTTCAAATTACCGTCTTTACGCTGCATACCGCTAAACAATTTTTTACCGGTAGCATCAGTGACCTCCAGCCAGCAATCGGCAGTAAAGTTCATCACCAGCACATTCGGATCAGCCACCGGCGTGGTCACGCCAGCCTGATCGGTTg                                                                                                        >  2:315603/1‑151 (MQ=255)

                                     ttAAGTTCAAATTACCGTCTTTACGCTGCATACCGCTAAACAATTTTTTACCGGTAGCATCAGTGACCTCCAGCCAGCAATCGGCAGTAAAGTTCATCACCAGCACATTCGGATCAGCCACCGGCGTGGTCACGCCAGCCTGATCGGTTgg                                                                                                       >  1:405397/1‑151 (MQ=255)

                                        aGTTCAAATTACCGTCTTTACGCTGCATACCGCTAAACAATTTTTTACCGGTAGCATCAGTGACCTCCAGCCAGCAATCGGCAGTAAAGTTCATCACCAGCACATTCGGATCAGCCACCGGCGTGGTCACGCCAGCCTGATCGGt                                                                                                          >  2:739452/1‑145 (MQ=255)

                                                  aCCGTCTTTACGCTGCATACCGCTAAACAATTTTTTACCGGTAGCATCAGTGACCTCCAGCCAGCAATCGGCAGTAAAGTTCATCACCAGCACATTCGGATCAGCCACCGGCGTGGTCACGCCAGCCTGATCGGTTGGCAACGGCgcagc                                                                                           >  2:398819/1‑150 (MQ=255)

                                                   ccGTCTTTACGCTGCATACCGCTAAACAATTTTTTACCGGTAGCATCAGTGACCTCCAGCCAGCAATCGGCAGTAAAGTTCATCACCAGCACATTCGGATCAGCCACCGGCGTGGTCACGCCAGCCTGATCGGTTGGCAACGGCGCAGCAc                                                                                         <  1:510954/151‑1 (MQ=255)

                                                    cGTCTTTACGCTGCATACCGCTAAACAATTTTTTACCGGTAGCATCAGTGACCTCCAGCCAGCAATCGGCAGTAAAGTTCATCACCAGCACATTCGGATCAGCCACCGGCGTGGTCACGCCAGCCTGATCGGTTGGCAACGGCGCAGCAcc                                                                                        >  2:142218/1‑151 (MQ=255)

                                                      tCTTTACGCTGCATACCGCTAAACAATTTTTTACCGGTAGCATCAGTGACCTCCAGCCAGCAATCGGCAGTAAAGTTCATCACCAGCACATTCGGATCAGCCACCGGCGTGGTCACGCCAGCCTGATCGGTTGGCAACGGCGCAGCACCAt                                                                                      >  1:481938/1‑151 (MQ=255)

                                                         ttACGCTGCATACCGCTAAACAATTTTTTACCGGTAGCATCAGTGACCTCCAGCCAGCAATCGGCAGTAAAGTTCATCACCAGCACATTCGGATCAGCCACCGGCGTGGTCACGCCAGCCTGATCGGTTGGCAACGGCGCAGCACCATc                                                                                     <  2:104032/149‑1 (MQ=255)

                                                                       gCTAAACAATTTTTTACCGGTAGCATCAGTGACCTCCAGCCAGCAATCGGCAGTAAAGTTCATCACCAGCACATTCGGATCAGCCACCGGCGTGGTCACGCCAGCCTGATCGGTTGGCAACGGCGCAGCACCATCTGGCGTTGTTGCTGcc                                                                     <  1:619396/151‑1 (MQ=255)

                                                                           aaCAATTTTTTACCGGTAGCATCAGTGACCTCCAGCCAGCAATCGGCAGTAAAGTTCATCACCAGCACATTCGGATCAGCCACCGGCGTGGTCACGCCAGCCTGATCGGTTGGCAACGGCGCAGCACCATCTGGCGTTGTTGCTGCCGt                                                                   >  1:599039/1‑149 (MQ=255)

                                                                              aaTTTTTTACCTGTAGCATCAGTGACCTCCAGCCAGCAATCGGCAGTAAAGTTCATCACCAGCGCATTCGGATCAGCCACCGGCGTGTTCACGCCAGCCTGATCTGTTGGCAACGGCGCAGCACCATCTGGCGTTGTTGCTGCCGTTGGTg                                                              <  1:343818/151‑1 (MQ=255)

                                                                                ttttttACCGGTAGCATCAGTGACCTCCAGCCAGCAATCGGCAGTAAAGTTCATCACCAGCACATTCGGATCAGCCACCGGCGTGGTCACGCCAGCCTGATCGGTTGGCAACGGCGCAGCACCATCTGGCGTTGTTGCTGCCGTTGGTGcc                                                            >  2:578225/1‑151 (MQ=255)

                                                                                ttttttACCGGTAGCATCAGTGACCTCCAGCCAGCAATCGGCAGTAAAGTTCATCACCAGCACATTCGGATCAGCCACCGGCGTGGTCACGCCAGCCTGATCGGTTGGCAACGGAGCAGCACCATCTGGCGTTGTTGCTGCCGTTGGTg                                                              >  1:701220/1‑149 (MQ=255)

                                                                                 tttttACCGGTAGCATCAGTGACCTCCAGCCAGCAATCCGCCGTAAAGTTCATCACCAGCACATTCGGCTCAGCCACCGGCGTGGTCACGCTAGCCTGATCGGTTGGCAACGGCGCAGCACCATCTGGCGTTGTGGCTGCCGTTGGTg                                                              >  1:614544/1‑148 (MQ=255)

                                                                                  ttttACCGGTAGCATCAGTGACCTCCAGCCAGCAATCGGCAGTAAAGTTCATCACCAGCACATTCGGATCAGCCACCGGCGTGGTCACGCCAGCCTGATCGGTTGGCAACGGCGCAGCACCATCTGGCGTTGTTGCTGCCGTTGGTGCCgg                                                          >  1:604571/1‑151 (MQ=255)

                                                                                      aCCGGTAGCATCAGTGACCTCCAGCCAGCAATCGGCAGTAAAGTTCATCACCAGCACATTCGGATCAGCCACCGGCGTGGTCACGCCAGCCTGATCGGTTGGCAACGGCGCAGCACCATCTGGCGTTGTTGCTGCCGTTGGTGCCGGGGTc                                                      <  2:498628/151‑1 (MQ=255)

                                                                                       ccGGTAGCATCAGTGACCTCCAGCCAGCAATCGGCAGTAAAGTTCATCACCAGCACATTCGGATCAGCCACCGGCGTGGTCACGCCAGCCTGATCGGTTGGCAACGGCGCAGCACCATCTGGCGTTGTTGCTGCCGTTGGTGCCGGGGTc                                                      >  1:823151/1‑150 (MQ=255)

                                                                                        cGGTAGCATCAGTGACCTCCAGCCAGCAATCGGCAGTAAAGTTCATCACCAGCACATTCGGATCAGCCACCGGCGTGGTCACGCCAGCCTGATCGGTTGGCAACGGCGCAGCACCATCTGGCGTTGTTGCTGCCGTTGGTGCCGGGGTcgc                                                    <  1:787626/151‑1 (MQ=255)

                                                                                        cGGTAGCATCAGTGACCTCCAGCCAGCAATCGGCAGTAAAGTTCATCACCAGCACATTCGGATCAGCCACCGGCGTGGTCACGCCAGCCTGATCGGTTGGCAACGGCGCAGCACCATCTGGCGTTGTTGCTGCCGTTGGTGCCGGGGTcgc                                                    <  1:391519/151‑1 (MQ=255)

                                                                                            aGCATCAGTGACCTCCAGCCAGCAATCGGCAGTAAAGTTCATCACCAGCACATTCGGATCAGCCACCGGCGTGGTCACGCCAGCCTGATCGGTTGGCAACGGCGCAGCACCATCTGGCGTTGTTGCTGCCGTTGGTGCCGGGGTCgcggcg                                                >  2:771632/1‑151 (MQ=255)

                                                                                            aGCATCAGTGACCTCCAGCCAGCAATCGGCAGTAAAGTTCATCACCAGCACATTCGGATCAGCCACCGGCGTGGTCACGCCAGCCTGATCGGTTGGCAACGGCGCAGCACCATCTGGCGTTGTTGCTGCCGTTGGTGCCGGGGTCgcggc                                                 <  1:139746/150‑1 (MQ=255)

                                                                                                      aCCTCCAGCCATCAAGCGGCAGTAAAGTTCATCACCAGCACATTCGGATCAGCCTCCGGCGTGGTGACGCCAGCCTGATCGGTTGGCAAAGGCGCAGCACCATCTGGCGTTGTTGCTGCCGTTGGTGCCGGTGTCGCGGCGGTa                                             <  1:216392/144‑1 (MQ=255)

                                                                                                       ccTCCAGCCAGCAATCGGCAGTAAAGTTCATCACCAGCACATTCGGATCAGCCACCGGCGTGGTCACGCCAGCCTGATCGGTTGGCAACGGCGCAGCACCATCTGGCGTTGTTGCTGCCGTTGGTGCCGGGGTCGCGGCGGTATCAACAt                                      <  2:512753/150‑1 (MQ=255)

                                                                                                          ccagccagCAATCGGCAGTAAAGTTCATCACCAGCACATTCGGATCAGCCACCGGCGTGGTCACGCCAGCCTGATCGGTTGGCAACGGCGCAGCACCATCTGGCGTTGTTGCTGCCGTTGGTGCCGGGGTCGCGGCGGTATCAACATTTGc                                  <  2:205523/151‑1 (MQ=255)

                                                                                                           cagccagcAATCGGCAGTAAAGTTCATCACCAGCACATTCGGATCAGCCACCGGCGTGGTCACGCCAGCCTGATCGGTTGGCAACGGCGCAGCACCATCTGGCGTTGTTGCTGCCGTTGGTGCCGGGGTCGCGGCGGTATCAACATTTGcc                                 >  2:621851/1‑151 (MQ=255)

                                                                                                                agcAATCGGCAGTAAAGTTCATCACCAGCACATTCGGATCAGCCACCGGCGTGGTCACGCCAGCCTGATCGGTTGGCAACGGCGCAGCACCATCTGGCGTTGTTGCTGCCGTTGGTGCCGGGGTCGCGGCGGTATCAACATTTGCCTGCGa                            <  1:578187/151‑1 (MQ=255)

                                                                                                                 gcAATCGGCAGTAAAGTTCATCACCAGCACATTCGGATCAGCCACCGGCGTGGTCACGCCAGCCTGATCGGTTGGCAACGGCGCAGCACCATCTGGCGTTGTTGCTGCCGTTGGTGCCGGGGTCGCGGCGGTATCAACATTTGCCTGCGaa                           <  2:820666/151‑1 (MQ=255)

                                                                                                                    aTCGGCAGTAAAGTTCATCACCAGCACATTCGGATCAGCCACCGGCGTGGTCACGCCAGCCTGATCGGTTGGCAACGGCGCAGCACCATCTGGCGTTGTTGCTGCCGTTGGTGCCGGGGTCGCGGCGGTATCAACATTTGCCTGCGAAGGc                        <  2:435367/151‑1 (MQ=255)

                                                                                                                      cGGCAGTAAAGTTCATCACCAGCACATTCGGATCAGCCACCGGCGTGGTCACGCCAGCCTGATCGGTTGGCAACGGCGCAGCACCATCTGGCGTTGTTGCTGCCGTTGGTGCCGGGGTCGCGGCGGTATCAACATTTGCCTGCGAAGGCGa                      <  1:608472/151‑1 (MQ=255)

                                                                                                                        gCAGTAAAGTTCATCACCAGCACATTCGGATCAGCCACCGGCGTGGTCACGCCAGCCTGATCGGTTGGCAACGGCGCAGCACCATCTGGCGTTGTTGCTGCCGTTGGTGCCGGGGTCGCGGCGGTATCAACATTTGCCTGCGAAGGCGaaa                    <  1:744535/151‑1 (MQ=255)

                                                                                                                          aGTAAAGTTCATCACCAGCACATTCGGATCAGCCACCGGCGTGGTCACGCCAGCCTGATCGGTTGGCAACGGCGCAGCACCATCTGGCGTTGTTGCTGCCGTTGGTGCCGGGGTCGCGGCGGTATCAACATTTGCCTGCGAAGGCGAaaca                  <  1:470208/151‑1 (MQ=255)

                                                                                                                            tAAAGTTCATCACCAGCACATTCGGATCAGCCACCGGCGTGGTCACGCCAGCCTGATCGGTTGGCAACGGCGCAGCACCATCTGGCGTTGTTGCTGCCGTTGGTGCCGGGGTCGCGGCGGTATCAACATTTGCCTGCGAAGGCGAaacaac                >  2:118008/1‑151 (MQ=255)

                                                                                                                              aaGTTCATCACCAGCACATTCGGATCAGCCACCGGCGTGGTCACGCCAGCCTGATCGGTTGGCAACGGCGCAGCACCATCTGGCGTTGTTGCTGCCGTTGGTGCCGGGGTCGCGGCGGTATCAACATTTGCCTGCGAAGGCGAAACAAcc               >  1:518301/1‑150 (MQ=255)

                                                                                                                                gTTCATCACCAGCACATTCGGATCAGCCACCGGCGGGGTCACGCCAGCCTGATCGGTTGGCAACGGCGCAGCACCATCTGGCGTTGTTGCTGCCGTTGGTGCCGGGGTCGCGGCGGTATCAACATTTGCCTGCGAAGGCGAAACAACCGCa            <  1:212519/151‑1 (MQ=255)

                                                                                                                                  tcatcaCCAGCACATTCGGATCAGCCACCGGCGTGGTCACGCCAGCCTGATCGGTTGGCAACGGCGCAGCACCATCTGGCGTTGTTGCTGCCGTTGGTGCCGGGGTCGCGGCGGTATCAACATTTGCCTGCGAAGGCGAAACAACCGCAtt          >  2:266652/1‑151 (MQ=255)

                                                                                                                                    atcaCCAGCACATTCGGATCAGCCACCGGCGTGGTCACGCCAGCCTGATCGGTTGGCAACGGCGCAGCACCATCTGGCGTTGTTGCTGCCGTTGGTGCCGGGGTCGCGGCGGTATCAACATTTGCCTGCGAAGGCGAAACAACCGCATTCt        <  1:184192/151‑1 (MQ=255)

                                                                                                                                         cAGCACATTCGGATCAGCCACCGGCGTGGTCACGCCAGCCTGATCGGTTGGCAACGGCGCAGCACCATCTGGCGTTGTTGCTGCCGTTGGTGCCGGGGTCGCGGCGGTATCAACATTTTCCTGCGAAGGCGAAACAACCGCATTCTGTTGc   >  1:227622/1‑151 (MQ=255)

                                                                                                                                          aGCACATTCGGATCAGCCACCGGCGTGGTCACGCCAGCCTGATCGGTTGGCAACGGCGCAGCACCATCTGGCGTTGTTGCTGCCGTTGGTGCCGGGGTCGCGGCGGTATCAACATTTGCCTGCGAAGGCGAAACAACCGCATTCTGTTGCg  <  2:462126/151‑1 (MQ=255)

GGCGCACCAATTTTCAGTTTGTACGGTGCCTGGCCGGTTAAGTTCAAATTACCGTCTTTACGCTGCATACCGCTAAACAATTTTTTACCGGTAGCATCAGTGACCTCCAGCCAGCAATCGGCAGTAAAGTTCATCACCAGCGCATTCGGATCAGCCACCGGCGTGGTCACGCCAGCCTGATCGGTTGGCAACGGCGCAGCACCATCTGGCGTTGTTGCTGCCGTTGGTGCCGGGGTCGCGGCGGTATCAACATTTGCCTGCGAAGGCGAAACAACCGCATTCTGTTGCG  >  NC_000913/2641943‑2642231

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A