Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A3 F50 I1 R1	300	54.7	1718999	98.4%	1691495	149.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,735,653	A→G	L120P (CTT→CCT)	rluD ←	23S rRNA pseudouridine(1911,1915,1917) synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,735,653	0	A	G	100.0%	194.7 / NA	52	L120P (CTT→CCT)	rluD	23S rRNA pseudouridine(1911,1915,1917) synthase
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (24/28); total (24/28)

ACGAGTAATTTCACGCCGTTGCAAAGATTCGACTAAACGCGTCTGAGCCGGAACGGTTTTTGCCACAACCATCAGGCCAGTGGTGTCTTTATCCAGACGATGGACGATGCCCGCACGCGGTACATCGGCAATGGGTGGATAGTAATGAAGCAACGCATTCAGTACCGTGCCATCCGGGTTACCCGCGCCAGGATGTACCACCAGGTCGCGCGGTTTATTAATGATAATAATGTCTTCATCTTCATAGACGATATCCAGCGGGATATCCTGCGGTTCAAAACGCGCTTCTTCT  >  NC_000913/2735505‑2735796

aCGAGTAATTTCACGCCGTTGCAAAGATTCGACTAAACGCGTCTGAGCCGGAACGGTTTTTGCCACAACCATCAGGCCAGTGGTGTCTTTATCCAGACGATGGACGATGCCCGCACGCGGTACATCGGCAATGGGTGGATAGTAATGAGGc                                                                                                                                               <  2:468063/151‑1 (MQ=255)

aCGAGTAATTTCACGCCGTTGCAAAGATTCGACTAAACGCGTCTGAGCCGGAACGGTTTTTGCCACAACCATCAGGCCAGTGGTGTCTTTATCCAGACGATGGACGATGCCCGCACGCGGTACATCGGCAATGGGTGGATAGTAATGAGGc                                                                                                                                               <  2:257226/151‑1 (MQ=255)

aCGAGTAATTTCACGCCGTTGCAAAGATTCGACTAAACGCGTCTGAGCCGGAACGGTTTTTGCCACAACCATCAGGCCAGTGGTGTCTTTATCCAGACGATGGACGATGCCCGCACGCGGTACATCGGCAATGGGTGGATAGTAATGAGGc                                                                                                                                               >  2:154914/1‑151 (MQ=255)

  gAGTAATTTCACGCCGTTGCAAAGATTCGACTAAACGCGTCTGAGCCGGAACGGTTTTTGCCACAACCATCAGGCCAGTGGTGTCTTTATCCAGACGATGGACGATGCCCGCACGCGGTACATCGGCAATGGGTGGATAGTAATGAGGCaa                                                                                                                                             <  1:781010/151‑1 (MQ=255)

    gTAATTTCACGCCGCTGCAAAGATTCGACTAAACGCGCCTGAGCCGGTACGGTTTTTGCCACAACCATCAGGCCAGTGTTGTCTTTATCCCTACGATGTACTATGCCCGTACGCGCTACCTCGGCCATGGGTGGATAGTACTGAGGCatc                                                                                                                                            >  1:371364/1‑148 (MQ=255)

          tCACGCCGTTGCAAAGATTCGACTAAACGCGTCTGAGCCGGAACGGTTTTTGCCACAACCATCAGGCCAGTGGTGTCTTTATCCAGACGATGGACGATGCCCGCACGCGGTACATCGGCAATGGGTGGATAGTAATGAGGCAACGCATTc                                                                                                                                      <  1:273821/150‑1 (MQ=255)

                cGTTGCAAAGATTCGACTAAACGCGTCTGAGCCGGAACGGTTTTTGCCACAACCATCAGGCCAGTGGTGTCTTTATCCAGACGATGGACGATGCCCGCACGCGGTACATCGGCAATGGGTGGATAGTAATGAGGCAACGCATTCAGTACCg                                                                                                                               >  1:631643/1‑151 (MQ=255)

                 gTTGCAAAGATTCGACTAAACGCGTCTGAGCCGGAACGGTTTTTGCCACAACCATCAGGCCAGTGGTGTCTTTATCCAGACGATGGACGATGCCCGCACGCGGTACATCGGCAATGGGTGGATAGTAATGAGGCAACGCATTCAGTACCGt                                                                                                                              <  1:432416/151‑1 (MQ=255)

                   tGCAAAGATTCGACTAAACGCGTCTGAGCCGGAACGGTTTTTGCCACAACCATCAGGCCAGTGGTGTCTTTATCCAGACGATGGACGATGCCCGCACGCGGTACATCGGCAATGGGTGGATAGTAATGAGGCAACGCAtt                                                                                                                                       <  1:482406/140‑1 (MQ=255)

                   tGCAAAGATTCGACTAAACGCGTCTGAGCCGGAACGGTTTTTGCCACAACCATCAGGCCAGTGGTGTCTTTATCCAGACGATGGACGATGCCCGCACGCGGTACATCGGCAATGGGTGGATAGTAATGAGGCAACGCATTCAGTACCGTGc                                                                                                                            >  1:163422/1‑151 (MQ=255)

                   tGCAAAGATTCGACTAAACGCGTCTGAGCCGGAACGGTTTTTGCCACAACCATCAGGCCAGTGGTGTCTTTATCCAGACGATGGACGATGCCCGCACGCGGTACATCGGCAATGGGTGGATAGTAATGAGGCAACGCATTCAGTACCGTGc                                                                                                                            >  2:120470/1‑151 (MQ=255)

                      aaaGATTCGACTAAACGCGTCTGAGCCGGAACGGTTTTTGCCACAACCATCAGGCCAGTGGTGTCTTTATCCAGACGATGGACGATGCCCGCACGCGGTACATCGGCAATGGGTGGATAGTAATGAGGCAACGCATTCAGTACCGTGCCAt                                                                                                                         >  2:793136/1‑151 (MQ=255)

                       aaGATTCGACTAAACGCGTCTGAGCCGGAACGGTTTTTGCCACAACCATCAGGCCAGTGGTGTCTTTATCCAGACGATGGACGATGCCCGCACGCGGTACATCGGCAATGGGTGGATAGTAATGAGGCAACGCATTCAGTACCGTGCCATc                                                                                                                        <  2:554694/151‑1 (MQ=255)

                       aaGATTCGACTAAACGCGTCTGAGCCGGAACGGTTTTTGCCACAACCATCAGGCCAGTGGTGTCTTTATCCAGACGATGGACGATGCCCGCACGCGGTACATCGGCAATGGGTGGATAGTAATGAGGCAACGCATTCAGTACCGTGCCATc                                                                                                                        <  2:391762/151‑1 (MQ=255)

                        aGATTCGACTAAACGCGTCTGAGCCGGAACGGTTTTTGCCACAACCATCAGGCCAGTGGTGTCTTTATCCAGACGATGGACGATGCCCGCACGCGGTACATCGGCAATGGGTGGATAGTAATGAGGCAACGCATTCAGTACCGTGCCATcc                                                                                                                       <  1:485615/151‑1 (MQ=255)

                        aGATTCGACTAAACGCGTCTGAGCCGGAACGGTTTTTGCCACAACCATCAGGCCAGTGGTGTCTTTATCCAGACGATGGACGATGCCCGCACGCGGTACATCGGCAATGGGTGGATAGTAATGAGGCAACGCATTCAGTACCGTGCCATcc                                                                                                                       <  1:566271/151‑1 (MQ=255)

                         gATTCGACTAAACGCGTCTGAGCCGGAACGGTTTTTGCCACAACCATCAGGCCAGTGGTGTCTTTATCCAGACGATGGACGATGCCCGCACGCGGTACATCGGCAATGGGTGGATAGTAATGAGGCAACGCATTCAGTACCGTGCCATCCg                                                                                                                      >  2:189794/1‑151 (MQ=255)

                          aTTCGACTAAACGCGTCTGAGCCGGAACGGTTTTTGCCACAACCATCAGGCCAGTGGTGTCTTTATCCAGACGATGGACGATGCCCGCACGCGGTACATCGGCAATGGGTGGATAGTAATGAGGCAACGCATTCAGTACCGTGCCATCCgg                                                                                                                     <  1:191425/151‑1 (MQ=255)

                            tCGACTAAACGCGTCTGAGCCGGAACGGTTTTTGCCACAACCATCAGGCCAGTGGTGTCTTTATCCAGACGATGGACGATGCCCGCACGCGGTACATCGGCAATGGGTGGATAGTAATGAGGCAACGCATTCAGTACCGTGCCATCCggg                                                                                                                    <  1:17364/150‑1 (MQ=255)

                              gACTAAACGCGTCTGAGCCGGAACGGTTTTTGCCACAACCATCAGGACAGTGGTGTCTTTATCCAGACGATGGACGATGCCCGCACGCGGTACATCGGCAATGGGTGGATAGTAATGAGGCAACGCATTCAGTACCGTGCCATCCGGGtt                                                                                                                  >  2:525707/1‑150 (MQ=255)

                                         tCTGAGCCGGAACGGTTTTTGCCACAACCATCAGGCCAGTGGTGTCTTTATCCAGACGATGGACGATGCCCGCACGCGGTACATCGGCAATGGGTGGATAGTAATGAGGCAACGCATTCAGTACCGTGCCATCCGGGTTACCCGCGCCAgg                                                                                                      >  1:173999/1‑151 (MQ=255)

                                          cTGAGCCGGAACGGTTTTTGCCACAACCATCAGGCCAGTGGTGTCTTTATCCAGACGATGGACGATGCCCGCACGCGGTACATCGGCAATGGGTGGATAGTAATGAGGCAACGCATTCAGTACCGTGCCATCCGGGTTACCCGCGCCAgg                                                                                                      >  2:48221/1‑150 (MQ=255)

                                             aGCCGGAACGGTTTTTGCCACAACCATCAGGCCAGTGGTGTCTTTATCCAGACGATGGACGATGCCCGCACGCGGTACATCGGCAATGGGTGGATAGTAATGAGGCAACGCATTCAGTACCGTGCCATCCGGGTTACCCGCGCCAGGATGt                                                                                                  >  2:364597/1‑151 (MQ=255)

                                                   aaCGGTTTTTGCCACAACCATCAGGCCAGTGGTGTCTTTATCCAGACGATGGTCGATGCCCGCACGCGGTACATCGGCAATGGGTGGATAGTAATGAGGCAACGCATTCAGTACCGTGCCATCCGGGTTACCCGCGCCAGGATGTaccacc                                                                                            >  1:515165/1‑151 (MQ=255)

                                                       gTTTTTGCCACAACCATCAGGCCAGTGGTGTCTTTATCCAGACGATGGACGATGCCCGCACGCGGTACATCGGCAATGGGTGGATAGTAATGAGGCAACGCATTCAGTACCGTGCCATCCGGGTTACCCGCGCCAGGATGTACCACCAGGt                                                                                        <  2:54230/151‑1 (MQ=255)

                                                         ttttGCCACAACCATCAGGCCAGTGGTGGCTTTATCCAGACGATGGACGATGCCCGCACGCGGTACATCGGCAATGGGTGGATAGTAATGAGGCAACGCATTCAGTACCGTGCCATCCGGGTTACCCGCGCCAGGATGTACCACCAGGTc                                                                                       <  1:160895/150‑1 (MQ=255)

                                                                 caACCATCAGGCCAGTGGTGTCTTTATCCAGACGATGGACGATGCCCGCACGCGGTACATCGGCAATGGGTGGATAGTAATGAGGCAACGCATTCAGTACCGTGCCATCCGGGTTACCCGCGCCAGGATGTACCACCAGGTCGCGCGGttt                                                                              <  1:682259/151‑1 (MQ=255)

                                                                  aaCCATCAGGCCAGTGGTGTCTTTATCCAGACGATGGACGATGCCCGCACGCGGTACATCGGCAATGGGTGGATAGTAATGAGGCAACGCATTCAGTACCGTGCCATCCGGGTTACCCGCGCCAGGATGTACCACCAGGTCGCGCGGttt                                                                              >  1:234779/1‑150 (MQ=255)

                                                                  aaCCATCAGGCCAGTGGTGTCTTTATCCAGACGATGGACGATGCCCGCACGCGGTACATCGGCAATGGGTGGATAGTAATGAGGCAACGCATTCAGTACCGTGCCATCCGGGTTACCCGCGCCAGGATGTACCACCAGGTCGCGCGGttt                                                                              >  2:743785/1‑150 (MQ=255)

                                                                    ccATCAGGCCAGTGGTGTCTTTATCCAGACGATGGACGATGCCCGCACGCGGTACATCGGCAATGGGTGGATAGTAATGAGGCAACGCATTCAGTACCGTGCCATCCGGGTTACCCGCGCCAGGATGTACCACCAGGTCGCGCGGTttatt                                                                           <  2:536994/151‑1 (MQ=255)

                                                                      aTCAGGCCAGTGGTGTCTTTATCCAGACGATGGACGATGCCCGCACGCGGTACATCGGCAATGGGTGGATAGTAATGAGGCAACGCATTCAGTACCGTGCCATCCGGGTTACCCGCGCCAGGATGTACCACCAGGTCGCGCGGTTTATTaa                                                                         <  1:527978/151‑1 (MQ=255)

                                                                        cAGGCCAGTGGTGTCTTTATCCAGACGATGGACGATGCCCGCACGCTGTACATCGGCAATGGGTGTATAGTAATGAGGCAACGCATTCAGTACCGTGCCATCCGGGTTACCCGCGCCAGGATGTACCACCAGGTCGCGCGGTTTATTAAt                                                                        <  1:793104/150‑1 (MQ=255)

                                                                              aGTGGTGTCTTTATCCAGACGATGGACGATGCCCGCACGCGGTACATCGGCAATGGGTGGATAGTAATGAGGCAACGCATTCAGTACCGTGCCATCCGGGTTACCCGCGCCAGGATGTACCACCAGGTCGCGCGGTTTATTAATGataata                                                                 <  1:214209/151‑1 (MQ=255)

                                                                                 ggtgTCTTTATCCAGACGATGGACGATGCCCGCACGCGGTACATCGGCAATGGGTGGATAGTAATGAGGCAACGCATTCAGTACCGTGCCATCCGGGTTACCCGCGCCGGGATGTACCACCAGGTCGCGCGGTTTATTAATGATaataat                                                               <  1:330290/150‑1 (MQ=255)

                                                                                    gtCTTTATCCAGACGATGGACGATGCCCGCACGCGGTACATCGGCAATGGGTGGATAGTAATGAGGCAACGCATTCAGTACCGTGCCATCCGGGTTACCCGCGCCAGGATGTACCACCAGGTCGCGCGGTTTATTAATGATAATAATGtct                                                           <  1:179279/151‑1 (MQ=255)

                                                                                    gtCTTTATCCAGACGATGGACGATGCCCGCACGCGGTACATCGGCAATGGGTGGATAGTAATGAGGCAACGCATTCAGTACCGTGCCATCCGGGTTACCCGCGCCAGGATGTACCACCAGGTCGCGCGGTTTATTAATGATAATAATGtct                                                           <  1:845298/151‑1 (MQ=255)

                                                                                          aTCCAGACGATGGACGATGCCCGCACGCGGTACATCGGCAATGGGTGGATAGTAATGAGGCAACGCATTCAGTACCGTGCCATCCGGGTTACCCGCGCCAGGATGTACCACCAGGTCGCGCGGTTTATTAATGATAATAATGtcttcatct                                                     >  2:751534/1‑151 (MQ=255)

                                                                                                            gCCCGCACGCGGTACATCGGCAATGGGTGGATAGTAATGAGGCAACGCATTCAGTACCGTGCCATCCGGGTTACCCGCGCCAGGATGTACCACCAGGTCGCGCGGTTTATTAATGATAATAATGTCTTCATCTTCATAGACGATATCCAGc                                   >  1:801038/1‑151 (MQ=255)

                                                                                                            gCCCGCACGCGGTACATCGGCAATGGGTGGATAGTAATGAGGCAACGCATTCAGTACCGTGCCATCCGGGTTACCCGCGCCAGGATGTACCACCAGGTAGCGCGGTTTATTAATGATAATAATGTCTTCATCTTCATAGACGATATCCAGc                                   >  1:544552/1‑151 (MQ=255)

                                                                                                               cGCACGCGGTACATCGGCAATGGGTGGATAGTAATGAGGCAACGCATTCAGTACCGTGCCATCCGGGTTACCCGCGCCAGGATGTACCACCAGGTCGCGCGGTTTATTAATGATAATAATGTCTTCATCTTCATAGACGATATCCAGCggg                                <  2:358003/151‑1 (MQ=255)

                                                                                                                gCACGCGGTACATCGGCAATGGGTGGATAGTAATGAGGCAACGCATTCAGTACCGTGCCATCCGGGTTACCCGCGCCAGGATGTACCACCAGGTCGCGCGGTTTATTAATGATAATAATGTCTTCATCTTCATAGACGATATCCAGCggg                                >  2:481288/1‑150 (MQ=255)

                                                                                                                   cgcgGTACATCGGCAATGGGTGGATAGTAATGAGGCAACGCATTCAGTACCGTGCCATCCGGGTTACCCGCGCCAGGATGTACCACCAGGTCGCGCGGTTTATTAATGATAATAATGTCTTCATCTTCATAGACGATATCCAGCGGGatat                            >  1:733610/1‑151 (MQ=255)

                                                                                                                      ggTACATCGGCAATGGGTGGATAGTAATGAGGCAACGCATTCAGTACCGTGCCATCCGGGTTACCCGCGCCAGGATGTACCACCAGGTCGCGCGGTTTATTAATGATAATAATGTCTTCATCTTCATAGACGATATCCAGCGGGATATCCt                         <  2:355137/151‑1 (MQ=255)

                                                                                                                         aCATCGGCAATGGGTGGATAGTAATGAGGCAACGCATTCAGTACCGTGCCATCCGGGTTACCCGCGCCAGGATGTACCACCAGGTCGCGCGGTTTATTAATGATAATAATGTCTTCATCTTCATAGACGATATCCAGCGGGATATCCTGCg                      <  2:419548/151‑1 (MQ=255)

                                                                                                                         aCATCGGCAATGGGTGGATAGTAATGAGGCAACGCATTCAGTACCGTGCCATCCGGGTTACCCGCGCCAGGATGTACCACCAGGTCGCGCGGTTTATTAATGATAATAATGTCTTCATCTTCATAGACGATATCCAGCGGGATATCCTGCg                      <  2:381708/151‑1 (MQ=255)

                                                                                                                            tCGGCAATGGGTGGATAGTAATGAGGCAACGCATTCAGTACCGTGCCATCCGGGTTACCCGCGCCAGGATGTACCACCAGGTCGCGCGGTTTATTAATGATAATAATGTCTTCATCTTCATAGACGATATCCAGCGGGATATCCTGCGGtt                   >  1:531414/1‑151 (MQ=255)

                                                                                                                              ggCAATGGGTGGATAATAATGAGGCAACGCATTCAGTACCGTGCCATCCGGGTTACCCGCGCCAGGATGTACCACCAGGTCGCGCGGTTTATTAATGATAATAATGTCTTCATCTTCATAGACGATATCCAGCGGGATATCCTGCGGTTc                  >  2:628988/1‑150 (MQ=255)

                                                                                                                               gCAATGGGTGGATAGTAATGAGGCAACGCATTCAGTACCGTGCCATCCGGGTTACCCGCGCCAGGATGTACCACCAGGTCGCGCGGTTTATTAATGATAATAATGTCTTCATCTTCATAGACGATATCCAGCGGGATATCCTGCGGTTCa                 >  1:773067/1‑150 (MQ=255)

                                                                                                                                  aTGGGTGGATAGTAATGAGGCAACGCATTCAGTACCGTGCCATCCGGGTTACCCGCGCCAGGATGTGCCACCAGGTCGCGCGGTTTATTAATGATAATAATGTCTTCATCTTCATAGACGATATCCAGCGGGATATCCTGCGGTTCAAAAc             <  1:702254/151‑1 (MQ=255)

                                                                                                                                           tAGTAATGAGGCAACGCATTCAGTACCGTGCCATCCGGGTTACCCGCGCCAGGATGTACCACCAGGTCGCGCGGTTTATTAATGATAATAATGTCTTCATCTTCATAGACGATATCCAGCGGGATATCCTGCGGTTCAAAACGCGcttct     <  1:133991/150‑1 (MQ=255)

                                                                                                                                            aGTAATGAGGCAACGCATTCAGTACCGTGCCATCCGGGTTACCCGCGCCAGGATGTACCACCAGGTCGCGCGGTTTATTAATGATAATAATGTCTTCATCTTCATAGACGATATCCAGCGGGATATCCTGCGGTTCAAAACGCGcttcttc   >  1:608237/1‑151 (MQ=255)

                                                                                                                                             gTAATGAGGCAACGCATTCAGTACCGTGCCATCCGGGTTACCCGCGCCAGGATGTACCACCAGGTCGCGCGGTTTATTAATGATAATAATGTCTTCATCTTCATAGACGATATCCAGCGGGATATCCTGCGGTTCAAAACGCGcttcttct  >  2:559807/1‑151 (MQ=255)

ACGAGTAATTTCACGCCGTTGCAAAGATTCGACTAAACGCGTCTGAGCCGGAACGGTTTTTGCCACAACCATCAGGCCAGTGGTGTCTTTATCCAGACGATGGACGATGCCCGCACGCGGTACATCGGCAATGGGTGGATAGTAATGAAGCAACGCATTCAGTACCGTGCCATCCGGGTTACCCGCGCCAGGATGTACCACCAGGTCGCGCGGTTTATTAATGATAATAATGTCTTCATCTTCATAGACGATATCCAGCGGGATATCCTGCGGTTCAAAACGCGCTTCTTCT  >  NC_000913/2735505‑2735796

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A