Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A3 F50 I1 R1	300	54.7	1718999	98.4%	1691495	149.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,811,185	T→C	L139P (CTC→CCC)	mprA →	transcriptional repressor of microcin B17 synthesis and multidrug efflux

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,811,185	0	T	C	100.0%	179.7 / NA	50	L139P (CTC→CCC)	mprA	transcriptional repressor of microcin B17 synthesis and multidrug efflux
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (21/29); total (21/29)

GTATTGCCGATGAACTGGAAAAACGCGGTTGGATCGAACGTCGTGAAAGCGATAACGATCGCCGCTGCCTGCATCTGCAATTAACGGAAAAAGGTCACGAGTTTTTGCGCGAGGTTTTACCACCGCAGCATAACTGCCTGCATCAACTCTGGTCCGCGCTCAGCACAACAGAAAAAGATCAGCTCGAGCAAATCACCCGCAAATTGCTCTCCCGTCTCGACCAGATGGAACAAGACGGTGTGGTTCTCGAAGCGATGAGCTAACGCGTCATCTCGCTCAAAAATCCAGATT  >  NC_000913/2811038‑2811328

gTATTGCCGATGAACTGGAAAAACGCGGTTGGATCGAACGTCGTGAAAGCGATAACGATCGCCGCTGCCTGCATCTGCAATTAACGGAAAAAGGTCACGAGTTTTTGCGCGAGGTTTTACCACCGCAGCATAACTGCCTGCATCAACCCTg                                                                                                                                              <  2:242002/151‑1 (MQ=255)

  aTTGCCGATGAACTGGAAAAACGCGGTTGGATCGAACGTCGTGAAAGCGATAACGATCGCCGCTGCCTGCATCTGCAATTAACGGAAAAAGGTCACGAGTTTTTGCGCGAGGTTTTACCACCGCAGCATAACTGCCTGCATCAACCCTGGt                                                                                                                                            >  2:741948/1‑151 (MQ=255)

          tGAACTGGAAAAACGCGGTTGGATCGAACGTCGTGAAAGCGATAACGATCGCCGCTGCCTGCATCTGCAATTAACGGAAAAAGGTCACGAGTTTTTGCGCGAGGTTTTACCACCGCAGCATAACTGCCTGCATCAACCCTGGTCCGCGCTc                                                                                                                                    >  2:536132/1‑151 (MQ=255)

           gAACTGGAAAAACGCGGTTGGATCGAACGTCGTGAAAGCGATAACGATCGCCGCTGCCTGCATCTGCAATTAACGGAAAAAGGTCACGAGTTTTTGCGCGAGGTTTTACCACCGCAGCATAACTGCCTGCATCAACCCTGGTCCGCGCTCa                                                                                                                                   <  1:741905/151‑1 (MQ=255)

            aaCTGGAAAAACGCGGTTGGATCGAACGTCGTGAAAGCGATAACGATCGCCGCTGCCTGCATCTGCAATTAACGGAAAAAGGTCACGAGTTTTTGCGCGAGGTTTTACCACCGCAGCATAACTGCCTGCATCAACCCTGGTCCGCGCTc                                                                                                                                    >  2:599710/1‑149 (MQ=255)

                   aaaaCGCGGTTGGATCGAACGTCGTGAAAGCGATAACGATCGCCGCTGCCTGCATCTGCAATTAACGGAAAAAGGTCACGAGTTTTTGCGCGAGGTTTTACCACCGCAGCATAACTGCCTGCATCAACCCTGGTCCGCGCTCAGCacaaca                                                                                                                           <  1:740661/151‑1 (MQ=255)

                   aaaaCGCGGTTGGATCGAACGTCGTGAAAGCGATAACGATCGCCGCTGCCTGCATCTGCAATTAACGGAAAAAGGTCACGAGTTTTTGCGCGAGGTTTTACCACCGCAGCATAACTGCCTGCATCAACCCTGGTCCGCGCTCAGCacaaca                                                                                                                           <  2:248971/151‑1 (MQ=255)

                         cgGTTGGATCGAACGTCGTGAAAGCGATAACGATCGCCGCTGCCTGCATCTGCAATTAACGGAAAAAGGTCACGAGTTTTTGCGCGAGGTTTTACCACCGCAGCATAACTGCCTGCATCAACCCTGGTCCGCGCTCAGCACAACAGaaaaa                                                                                                                     <  1:358324/151‑1 (MQ=255)

                          ggTTGGATCGAACGTCGTGAAAGCGATAACGATCGCCGCTGCCTGCATCTGCAATTAACGGAAAAAGGTCACGAGTTTTTGCGCGAGGTTTTACCACCGCAGCATAACTGCCTGCATCAACCCTGGTCCGCGCTCAGCACAACAGAAAAAg                                                                                                                    <  2:213231/151‑1 (MQ=255)

                             tGGATCGAACGTCGTGAAAGCGATAACGATCGCCGCTGCCTGCATCTGCAATTAACGGAAAAAGGTCACGAGTTTTTGCGCGAGGTTTTACCACCGCAGCATAACTGCCTGCATCAACCCTGGTCCGCGCTCAGCACAACAGaaaa                                                                                                                      >  2:179362/1‑146 (MQ=255)

                               gATCGAACGTCGTGAAAGCGATAACGATCGCCGCTGCCTGCATCTGCAATTAACGGAAAAAGGTCACGAGTTTTTGCGCGAGGTTTTACCACCGCAGCATAACTGCCTGCATCAACCCTGGTCCGCGCTCAGCACAACAGAAAAAGATCAg                                                                                                               <  2:777153/151‑1 (MQ=255)

                                    aaCGTCGTGAAAGCGATAACGATCGCCGCTGCCTGCATCTGCAATTAACGGAAAAAGGTCACGAGTTTTTGCGCGAGGTTTTACCACCGCAGCATAACTGCCTGCATCAACCCTGGTCCGCGCTCAGCACAACAGAAAAAGATCAGCTCg                                                                                                           >  1:462310/1‑150 (MQ=255)

                                    aaCGTCGTGAAAGCGATAACGATCGCCGCTGCCTGCATCTGCAATTAACGGAAAAAGGTCACGAGTTTTTGCGCGAGGTTTTACCACCGCAGCATAACTGCCTGCATCAACCCTGGTCCGCGCTCAGCACAACAGAAAAAGATCAGCTCGa                                                                                                          <  1:149576/151‑1 (MQ=255)

                                    aaCGTCGTGAAAGCGATAACGATCGCCGCTGCCTGCATCTGCAATTAACGGAAAAAGGTCACGAGTTTTTGCGCGAGGTTTTACCACCGCAGCATAACTGCCTGCATCAACCCTGGTCCGCGCTCAGCACAACAGAAAAAGATCAGCTCGa                                                                                                          <  2:855100/151‑1 (MQ=255)

                                       gtcgtGAAAGCGATAACGATCGCCGCTGCCTGCATCTGCAATTAACGGAAAAAGGTCACGAGTTTTTGCGCGAGGTTTTACCACCGCAGCATAACTGCCTGCATCAACCCTGGTCCGCGCTCAGCACAACAGAAAAAGATCAGCTCGAGCa                                                                                                       <  2:216744/151‑1 (MQ=255)

                                         cgtGAAAGCGATAACGATCGCCGCTGCCTGCATCTGCAATTAACGGAAAAAGGTCACGAGTTTTTGCGCGAGGTTTTACCACCGCAGCATAACTGCCTGCATCAACCCTGGTCCGCGCTCAGCACAACAGAAAAAGATCAGCTCGAGCaa                                                                                                      <  2:339186/150‑1 (MQ=255)

                                          gtGAAAGCGATAACGATCGCCGCTGCATGCATCTGCAATTAACGGAAAAAGGTCACGAGTTTTTGCGCGAGGTTTTACCACCGCAGAATAACTGCCTGCATCAAACCTGGTCCGCGCTCAGCACAACAGAAAAAGATCAGCCCGAGCaa                                                                                                      >  1:592765/1‑149 (MQ=255)

                                           tGAAAGCGATAACGATCGCCGCTGCCTGCATCTGCAATTAACGGAAAAAGGTCACGAGTTTTTGCGCGAGGTTTTACCACCGCAGCATAACTGCCTGCATCAACCCTGGTCCGCGCTCAGCACAACAGAAAAAGATCAGCTCGAGCAAATc                                                                                                   >  2:785854/1‑151 (MQ=255)

                                                   aTAACGATCGCCGCTGCCTGCATCTGCAATTAACGGAAAAAGGTCACGAGTTTTTGCGCGAGGTTTTACCACCGCAGCATAACTGCCTGCATCAACCCTGGTCCGCGCTCAGcac                                                                                                                               <  2:374067/115‑1 (MQ=255)

                                                   aTAACGATCGCCGCTGCCTGCATCTGCAATTAACGGAAAAAGGTCACGAGTTTTTGCGCGAGGTTTTACCACCGCAGCATAACTGCCTGCATCAACCCTGGTCCGCGCTCAGcac                                                                                                                               >  1:374042/1‑115 (MQ=255)

                                                   aTAACGATCGCCGCTGCCTGCATCTGCAATTAACGGAAAAAGGTCACGAGTTTTTGCGCGAGGTTTTACCACCGCAGCATAACTGCCTGCATCAACCCTGGTCCGCGCTCAGCACAACAGAAAAAGATCAGCTCGAGCAAATCACCCGCa                                                                                            >  1:638470/1‑150 (MQ=255)

                                                     aaCGATCGCCGCTGCCTGCATCTGCAATTAACGGAAAAAGGTCACGAGTTTTTGCGCGAGGTTTTACCACCGCAGCATAACTGCCTGCATCAACCCTGGTCCGCGCTCAGCACAACAGAAAAAGATCAGCTCGAGCAAATCACCCGCaaa                                                                                          <  1:36400/150‑1 (MQ=255)

                                                     aaCGATCGCCGCTGCCTGCATCTGCAATTAACGGAAAAAGGTCACGAGTTTTTGCGCGAGGTTTTACCACCGCAGCATAACTGCCTGCATCAACCCTGGTCCGCGCTCAGCACAACAGAAAAAGATCAGCTCGAGCAAATCACCCGCAAAt                                                                                         >  2:561278/1‑151 (MQ=255)

                                                     aaCGATCGCCGCTGCCTGCATCTGCAATTAACGGAAAAAGGTCACGAGTTTTTGCGCGAGGTTTTACCACCGCAGCATAACTGCCTGCATCAACCCTGGTCCGCGCTCAGCACAACAGAAAAAGATCAGCTCGAGCAAATCACCCGCAAAt                                                                                         >  2:283933/1‑151 (MQ=255)

                                                       cGATCGCCGCTGCCTGCATCTGCAATTAACGGAAAAAGGTCACGAGTTTTTGCGCGAGGTTTTACCACCGCAGCATAACTGCCTGCATCAACCCTGGTCCGCGCTCAGCACAACAGAAAAAGATCAGCTCGAGCAAATCACCCGCAAATTg                                                                                       <  1:617145/151‑1 (MQ=255)

                                                            gccgcTGCCTGCATCTGCAATTAACGGAAAAAGGTCACGAGTTTTTGCGCGAGGTTTTACCACCGCAGCATAACTGCCTGCATCAACCCTGGTCCGCGCTCAGCACAACAGAAAAAGATCAGCTCGAGCAAATCACCCGCAAATTGCtctc                                                                                  >  2:282930/1‑151 (MQ=255)

                                                               gcTGCCTGCATCTGCAATTAACGGAAAAAGGTCACGAGTTTTTGCGCGAGGTTTTACCACCGCAGCATAACTGCCTGCATCAACCCTGGTCCGCGCTCAGCACAACAGAAAAAGATCAGCTCGAGCAAATCACCCGCAAATTGCTCTCCCg                                                                               >  1:243276/1‑151 (MQ=255)

                                                                      gcATCTGCAATTAACGGAAAAAGGTCACGAGTTTTTGCGCGAGGTTTTACCACCGCAGCATAACTGCCTGCATCAACCCTGGTCCGCGCTCAGCACAACAGAAAAAGATCAGCTCGAGCAAATCACCCGCAAATTGCTCTCCCGTCTCGa                                                                         <  1:15563/150‑1 (MQ=255)

                                                                            gCAATTAACGGAAAAAGGTCACGAGTTTTTGCGCGAGGTTTTACCACCGCAGCATAACTGCCTGCATCAACCCTGGTCCGCGCTCAGCACAACAGAAAAAGATCAGCTCGAGCAAATCACCCGCAAATTGCTCTCCCGTCTCGACCAg                                                                     <  1:354985/148‑1 (MQ=255)

                                                                                  aaCGGAAAAAGGTCACGAGTTTTTGCGCGAGGTTTTACCACCGCAGCATAACTGCCTGCATCAACCCTGGTCCGCGCTCAGCACAACAGAAAAAGATCAGCTCGAGCAAATCACCCGCAAATTGCTCTCCCGTCTCGACCAGATGGAACaa                                                            <  2:394300/151‑1 (MQ=255)

                                                                                    cGGAAAAAGGTCACGAGTTTTTGCGCGAGGTTTTACCACCGCAGCATAACTGCCTGCATCAACCCTGGTCCGCGCTCAGCACAACAGAAAAAGATCAGCTCGAGCAAATCACCCGCAAATTGCTCTCCCGTCTCGACCAGATGGAACa                                                             >  2:151178/1‑148 (MQ=255)

                                                                                       aaaaaGGTCACGAGTTTTTGCGCGAGGTTTTACCACCGCAGCATAACTGCCTGCATCAACCCTGGTCCGCGCTCAGCACAACAGAAAAAGATCAGCTCGAGCAAATCACCCGCAAATTGCTCTCCCGTCTCGACCAGATGGAACAAGACgg                                                       <  1:599671/151‑1 (MQ=255)

                                                                                           aGGTCACGAGTTTTTGCGCGAGGTTTTACCACCGCAGCATAACTGCCTGCATCAACCCTGGTCCGCGCTCAGCACAACAGAAAAAGATCAGCTCGAGCAAATCACCCGCAAATTGCTCTCCCGTCTCGACCAGATGGAACAAGACGGtgtg                                                   >  2:248382/1‑151 (MQ=255)

                                                                                                 cGAGTTTTTGCGCGAGGTTTTACCACCGCAGCATAACTGCCTGCATCAACCCTGGTCCGCGCTCAGCACAACAGAAAAAGATCAGCTCGAGCAAATCACCCGCAAATTGCTCTCCCGTCTCGACCAGATGGAACAAGACGGTGTGGTtctc                                             <  2:847104/151‑1 (MQ=255)

                                                                                                       tttGCGCGAGGTTTTACCACCGCAGCATAACTGCCTGCATCAACCCTGGTCCGCGCTCAGCACAACAGAAAAAGATCAGCTCGAGCAAATCACCCGCAAATTGCTCTCCCGTCTCGACCAGATGGAACAAGACGGTGTGGTTCTCGAAGc                                        <  2:638512/150‑1 (MQ=255)

                                                                                                        ttGCGCGAGGTTTTACCACCGCAGCATAACTGCCTGCATCAACCCTGGTCCGCGCTCAGCACAACAGAAAAAGATCAGCTCGAGCAAATCACCCGCAAATTGCTCTCCCGTCTCGACCAGATGGAACAAGACGGTGTGGTTCTCGAAGCg                                       >  2:533696/1‑150 (MQ=255)

                                                                                                           cgcgAGGTTTTACCACCGCAGCATAACTGCCTGCATCAACCCTGGTCCGCGCTCAGCACAACAGAAAAAGATCAGCTCGAGCAAATCACCCGCAAATTGCTCTCCCGTCTCGACCAGATGGAACAAGACGGTGTGGTTCTCGAAGCGATg                                    >  1:384314/1‑150 (MQ=255)

                                                                                                           cgcgAGGTTTTACCACCGCAGCATAACTGCCTGCATCAACCCTGGTCCGCGCTCAGCACAACAGAAAAAGATCAGCTCGAGCAAATCACCCGCAAATTGCTCTCCCGTCTCGACCAGATGGAACAAGACGGTGTGGTTCTCGAAGCGATGa                                   <  2:229679/151‑1 (MQ=255)

                                                                                                            gcgAGGTTTTACCACCGCAGCATAACTGCCTGCATCAACCCTGGTCCGCGCTCAGCACAACAGAAAAAGATCAGCTCGAGCAAATCACCCGCAAATTGCTCTCCCGTCTCGACCAGATGGAACAAGACGGTGTGGTTCTCGAAGCGATg                                    >  2:485520/1‑149 (MQ=255)

                                                                                                                   tttACCACCGCAGCATAACTGCCTGCATCAACCCTGGTCCGCGCTCAGCACAACAGAAAAAGATCAGCTCGAGCAAATCACCCGCAAATTGCTCTCCCGTCTCGACCAGATGGAACAAGACGGTGTGGTTCTCGAAGCGATGAGCTAAcg                            <  2:731620/150‑1 (MQ=255)

                                                                                                                     tACCACCGCAGCATAACTGCCTGCATCAACCCTGGTCCGCGCTCAGCACAACAGAAAAAGATCAGCTCGAGCAAATCACCCGCAAATTGCTCTCCCGTCTCGACCAGATGGAACAAGACGGTGTGGTTCTCGAAGCGATGAGCTAAcgcg                          <  1:326877/150‑1 (MQ=255)

                                                                                                                           cGCAGCATAACTGCCTGCATCAACCCTGGTCCGCGCTCAGCACAACAGAAAAAGATCAGCTCGAGCAAATCACCCGCAAATTGCTCTCCCGTCTCGACCAGATGGAACAAGACGGTGTGGTTCTCGAAGCGATGAGCTAACGCGTCAtctc                   <  2:581083/151‑1 (MQ=255)

                                                                                                                             cagcaTAACTGCCTGCATCAACCCTGGTCCGCGCTCAGCACAACAGAAAAAGATCAGCTCGAGCAAATCACCCGCAAATTGCTCTCCCGTCTCGACCAGATGGAACAAGACGGTGTGGTTCTCGAAGCGATGAGCTAACGCGTCATCTCGc                 >  2:168280/1‑151 (MQ=255)

                                                                                                                              agcaTAACTGCCTGCATCAACCCTGGTCCGCGCTCAGCACAACAGAAAAAGATCAGCTCGAGCAAATCACCCGCAAATTGCTCTCCCGTCTCGACCAGATGGAACAAGACGGTGTGGTTCTCGAAGCGATGAGCTAACGCGTCATCTCGCt                <  2:540152/151‑1 (MQ=255)

                                                                                                                               gcaTAACTGCCTGCATCAACCCTGGTCCGCGCTCAGCACAACAGAAAAAGATCAGCTCGAGCAAATCACCCGCAAATTGCTCTCCCGTCTCGACCAGATGGAACAAGACGGTGTGGTTCTCGAAGCGATGAGCTAACGCGTCATCTCGCTc               >  2:205500/1‑151 (MQ=255)

                                                                                                                                       gcctgcATCAACCCTGGTCCGCGCTCAGCACAACAGAAAAAGATCAGCTCGAGCAAATCACCCGCAAATTGCTCTCCCGTCTCGACCAGATGGAACAAGACGGTGTGGTTCTCGAAGCGATGAGCTAACGCGTCATCTCGCTCAAAAATcc       <  2:363342/151‑1 (MQ=255)

                                                                                                                                          tgcATCAACCCTGGTCCGCGCTCAGCACAACAGAAAAAGATCAGCTCGAGCAAATCACCCGCAAATTGCTCTCCCGTCTCGACCAGATGGAACAAGACGGTGTGGTTCTCGAAGCGATGAGCTAACGCGTCATCTCGCTCAAAAATcc       >  1:762362/1‑148 (MQ=255)

                                                                                                                                           gcATCAACCCTGGTCCGCGCTCAGCACAACAGAAAAAGATCAGCTCGAGCAAATCACCCGCAAATTGCTCTCCCGTCTCGACCAGATGGAACAAGACGGTGTGGTTCTCGAAGCGATGAGCTAACGCGTCATCTCGCTCAAAAATCCAg     <  2:205646/149‑1 (MQ=255)

                                                                                                                                           gcATCAACCCTGGTCCGCGCTCAGCACAACAGAAAAAGATCAGCTCGAGCAAATCACCCGCAAATTGCTCTCCCGTCTCGACCAGATGGAACAAGACGGTGTGGTTCTCGAAGCGATGAGCTAACGCGTCATCTCGCTCAAAAATCCAGAt   <  1:812125/151‑1 (MQ=255)

                                                                                                                                            cATCAACCCTGGTCCGCGCTCAGCACAACAGAAAAAGATCAGCTCGAGCAAATCACCCGCAAATTGCTCTCCCGTCTCGACCAGATGGAACAAGACGGTGTGGTTCTCGAAGCGATGAGCTAACGCGTCATCTCGCTCAAAAATCCAGAtt  <  2:690472/151‑1 (MQ=255)

GTATTGCCGATGAACTGGAAAAACGCGGTTGGATCGAACGTCGTGAAAGCGATAACGATCGCCGCTGCCTGCATCTGCAATTAACGGAAAAAGGTCACGAGTTTTTGCGCGAGGTTTTACCACCGCAGCATAACTGCCTGCATCAACTCTGGTCCGCGCTCAGCACAACAGAAAAAGATCAGCTCGAGCAAATCACCCGCAAATTGCTCTCCCGTCTCGACCAGATGGAACAAGACGGTGTGGTTCTCGAAGCGATGAGCTAACGCGTCATCTCGCTCAAAAATCCAGATT  >  NC_000913/2811038‑2811328

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A