Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A3 F50 I1 R1
|
300 |
54.7 |
1718999 |
98.4% |
1691495 |
149.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
2,817,861 |
A→G |
intergenic (‑1/+198) |
argQ ← / ← argZ |
tRNA‑Arg/tRNA‑Arg |
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 2,817,861 | 0 | A | G | 87.9%
| 138.9
/ 6.7
| 58 | intergenic (‑1/+198) | argQ/argZ | tRNA‑Arg/tRNA‑Arg |
| Reads supporting (aligned to +/- strand): ref base A (5/2); new base G (19/32); total (24/34) |
| Fisher's exact test for biased strand distribution p-value = 1.14e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01 |
ATCCTTCCTGGTGCCTGCGGTTAATACCGCTATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTC‑GCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGG > NC_000913/2817713‑2818007
|
aTCCTTCCTGGTGCCTGCGGTTAATACCGCTATCTCTTCGATACCTTCATTGCTGAAGGTTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATc < 1:152900/151‑1 (MQ=255)
cttcctGGTGCCTGCGGTTAATACCGCCATCTCTTCGATACCTTCATTGCTGAAGGTTAGGGGGAATATGGATCATCCGGGCGGCTTGGAACCTCCGAGCGCTCGGTTCGGAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCGTCggg > 1:331324/1‑151 (MQ=14)
tcctGGTGCCTGCGGTTAATACCGCCATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCGTCggg < 1:156521/149‑1 (MQ=255)
tcctGGTGCCTGCGGTTAATACCGCCATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCGTCGGGa < 1:75238/150‑1 (MQ=255)
tGGTGCCTGCGGTTAATACCGCCATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCg > 1:474590/1‑144 (MQ=255)
gTGCCTGCGGTTAATACCGCCATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCGTCGGGAAAACtt < 1:773637/151‑1 (MQ=255)
gCCTGCGGTTAATACCGCCATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCGTCGGGAAAACTTAt < 2:585723/151‑1 (MQ=255)
gCCTGCGGTTAATACCGCCATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCGTCGGGAAAACTTAt < 1:57936/151‑1 (MQ=255)
cGGTTAATACCGCCATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCGTCGGGAAAACTTATTCTAc < 2:64756/151‑1 (MQ=255)
tAATACCGCCATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCGTCGGGAAAACTTATTCTActgc < 2:728495/150‑1 (MQ=255)
tACCGCCATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAACTTACTTTActgccggtt > 1:177996/1‑146 (MQ=11)
aCCGCCATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCGTCGGGAAAACTTATTCTACTGCTGa‑‑ta < 2:635615/151‑1 (MQ=255)
aCCGCCATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCGTCGGGAAAACTTATTCTACTGCTGa‑‑t < 2:554216/150‑1 (MQ=255)
ccGCCATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATAGGGagatgtagttgtgt > 1:740301/1‑129 (MQ=11)
aTCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCGTCGGGAAAATTTATTCTActgcggttttt > 2:65601/1‑140 (MQ=255)
ctctTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCGTCGGGAAAA‑TTATTTTACTGCCGA‑‑tttttggatcc > 1:321375/1‑144 (MQ=11)
ctTCGATACCTTCATTGCTGAAGGTTACGGTGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCGTCGGGAAAACTTATTCTACTGCTGA‑‑TATTGATACCGc > 2:399484/1‑151 (MQ=255)
ctTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCGTCGGGAAAACTTATTCTACTGCTGA‑‑TATTGATa < 1:788657/147‑1 (MQ=255)
ttCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCGTCGGGAAAACTTATTCTACTGCTGA‑‑TATTGATACCGc > 2:617907/1‑150 (MQ=255)
aTACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAACTTACTTTACTGCAGATTTTTTGATACCGctact < 1:616883/151‑1 (MQ=11)
tCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCGTCGGGAAAACTTATTCTACTGCTGA‑‑TATTGATACCGCTACTAAAGc < 2:807840/149‑1 (MQ=255)
tGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCGTCGGGAAAACTTATTCTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCat > 2:479289/1‑148 (MQ=255)
gCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCGTCGGGAAAACTTATTCTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCa < 1:449910/151‑1 (MQ=255)
gCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCGTCGGGAAAACTTATTCTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCa < 1:807606/151‑1 (MQ=255)
tACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCGTCGGGAAAACTTATTCTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAATTAagag < 1:231267/150‑1 (MQ=255)
tACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCGTCGGGAAAACTTATTCTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAATTAag > 1:392865/1‑148 (MQ=255)
atatGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCGTCGGGAAAACTTATTCTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCAt < 2:392897/151‑1 (MQ=255)
ataggtggCATCCGGGAGAATTCGAACCTCCGGCCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCGTCTGGAAACCTTATTCTACTGTTGA‑‑TATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCAt < 1:562496/144‑1 (MQ=14)
tatGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCCGGCAAGGCGACCTGCGGGGGCAGAGGGAAAAGTTCTTCTATTGCTGA‑‑TATTGATTCCCCCACTAAAGCCATTACAAATAAGGAATgggggctc > 1:111337/1‑145 (MQ=2)
ggTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCGTCGGGAAAACTTATTCTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCgg < 2:851578/151‑1 (MQ=255)
gTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCGTCGGGAAAACTTATTCTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCggg < 2:472027/151‑1 (MQ=255)
aTCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCGTCGGGAAAACTTATTCTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGggagg > 1:494253/1‑150 (MQ=255)
ccGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCGTCGGGAAAACTTATTCTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGAtt < 1:396471/151‑1 (MQ=255)
ccGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCGTCGGGAAAACTTATTCTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGAt < 2:639083/150‑1 (MQ=255)
cGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCGTCGGGAAAACTTATTCTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGAtt > 2:181856/1‑150 (MQ=255)
ggaggaTTCGAACCACCGACCGCTCGGTTCGTAGCCGAGTACTCTAGCCAGCTGAGCTACGGATGCGTCGGGAAAACTTATTCTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACt < 2:48717/151‑1 (MQ=255)
aggaTTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGCTGCGCCGGGCAGACCTTTTCTACTGCTGA‑‑TCTCGCAACCCCCACTCAAACGAAATCAATCAATAGAAGGTGCATCCGGTAAGAtacctc > 1:332003/1‑145 (MQ=9)
aggaTTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCGTCGGGAAAACTTATTCTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCg < 1:248851/151‑1 (MQ=255)
ggaTTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCGTCGGGAAAACTTATTCTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGc < 1:283469/151‑1 (MQ=255)
gaTTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCGTCGGGAAAACTTATTCTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGcc < 2:850123/151‑1 (MQ=255)
aTTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCGTCGGGAAAACTTATTCTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCt > 2:47711/1‑151 (MQ=255)
ccGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCGTCGGGAAAACTTATTCTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTC‑GCCCt < 1:47689/150‑1 (MQ=255)
ggTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCGTCGGGAAAACTTATTCTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTC‑GCCCTTCGGGcc < 2:241432/151‑1 (MQ=255)
ggTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCGTCGGGAAAACTTATTCTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGGGCATCCGGGGGGATTACTCGCCTTTCgtccggaaaggcggg > 1:227630/1‑135 (MQ=17)
cgtaaaggAGTACTCTTTTCAGAAGAGTTGCGGATCCGGCGGCAAAAATTATTATCCTACTAA‑‑TATTGATACCGCTACAAAAGCCATTTCAATTAAGAGATGGTGCATCAGGGAGGATTACTCGCCTGGCGGCTC‑GCCCTTCGGTCCGTTg < 1:684141/144‑1 (MQ=255)
ccGAGTACTCTATCCAGCTGAGCTACGGATGCGTCGGGAAAACTTATTCTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTC‑GCCCTTCGGGCCGTTGCTaa < 2:465791/150‑1 (MQ=255)
cGAGTACTCTATCCAGCTGAGCTACGGATGCGTCGGGAAAACTTATTCTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTC‑GCCCTTCGGGCCGTTGCTAAAg < 2:477258/151‑1 (MQ=255)
aCTCTATCCAGCTGAGCTACGGATGCGTCGGGAAAACTTATTCTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTC‑GCCCTTCGGGCCGTTGCTaaaacca > 2:452807/1‑145 (MQ=255)
aTCCAGCTGAGCTACGGATGCGTCGGGAAAATTAATTTTACGGCTAT‑‑TTTTGATACCGCTCCTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATCACTCCCCCGGCCGCTC‑GGTCTTCCGGCCGGTTCTAAACCaagct > 2:194034/1‑144 (MQ=9)
ccAGCTGAGCTACGGATGCGTCGGGAAAACTTATTCTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGGCCCTTC‑GGCCGGTGGCAAAGGAACGGTat > 1:123814/1‑148 (MQ=17)
ccAGCTGAGCTACGGATGCGTCGGGAAAACTTATTCTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTC‑GCCCTTCGGGCCGGTGCTTAAACAACGGTatcc > 1:165544/1‑149 (MQ=17)
cAGCTGAGCTACGGATGCGTCGGGAAAACTTATTCTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTC‑GCCCTTCGGGCCGTTGCTAAAGCAACGTTatcatc < 2:261273/151‑1 (MQ=255)
gAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTC‑GCCCTTCGGGCCGTTGCTAAAGCAACGTTATCCTCCCtgg > 2:92226/1‑148 (MQ=11)
aGCTACGGATGCGTCGGGAAAACTTATTCTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTC‑GCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGt < 2:473690/151‑1 (MQ=255)
cTACGGATGCGTCGGGAAAACTTATTCTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTC‑GCCCTTCCGGCCGTTTCCAAAGCAACGTTATCCTCCCCAg > 1:209650/1‑148 (MQ=17)
gATGCGTCGGGAAAACTTATTCTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTC‑CCCCTTCTCGCCGGTGGTTAAGCCACGCTGTCCTCCCTGGTccccg > 1:418443/1‑147 (MQ=17)
aTGCGTCGGGAAAACTTATTCTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTC‑GCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCgg < 2:227655/151‑1 (MQ=255)
aTGCGTCGGGAAAACTTATTCTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTC‑GCCCTTCGGGCCGTGGCTTAAACACAGTTATCATCCCTAGGCCCTGCgg > 1:129317/1‑151 (MQ=18)
|
ATCCTTCCTGGTGCCTGCGGTTAATACCGCTATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTC‑GCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGG > NC_000913/2817713‑2818007
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A