Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A3 F50 I1 R1	300	54.7	1718999	98.4%	1691495	149.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,955,448	T→C	T186A (ACC→GCC)	recB ←	exonuclease V (RecBCD complex), beta subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,955,448	0	T	C	100.0%	182.0 / NA	50	T186A (ACC→GCC)	recB	exonuclease V (RecBCD complex), beta subunit
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (21/29); total (21/29)

TGCTGTTTTACCGTATCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGTTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAGATAACGATTAATATCGCGCAGCAACGCCTGCGGCCCTTTCCAGGTTTCAAAGACGACCTGGGCTATTTCACGCGGCAGCGGGTAGCAGTGGCGACGCCAGAAATCGGCGCAGGCCTGGTAGCGTAGCAGAGACTCATCTTCAATCAGCTGCTGCTCAAACAGCATGCCGGATTCAAAGGCATTCAGGTTGA  >  NC_000913/2955300‑2955594

tgctgTTTTACCGTATCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGTTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAGATAACGATTAATATCGCGCAGCAACGCCTGCGGCCCTTTCCAGGctt                                                                                                                                                  >  2:526431/1‑151 (MQ=255)

 gctgTTTTACCGTATCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGTTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAGATAACGATTAATATCGCGCAGCAACGCCTGCGGCCCTTTCCAGGCTTc                                                                                                                                                 >  1:407729/1‑151 (MQ=255)

  ctgTTTTACCGTATCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGTTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAGATAACGATTAATATCGCGCAGCAACGCCTGCGGCCCTTTCCAGGCTTc                                                                                                                                                 >  1:355003/1‑150 (MQ=255)

      tttACCGTATCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGTTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAGATAACGATTAATATCGCGCAGCAACGCCTGCGGCCCTTTCCAGGCTTCAAAg                                                                                                                                             <  1:628176/150‑1 (MQ=255)

            gTATCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGTTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAGATAACGATTAATATCGCGCAGCAACGCCTGCGGCCCTTTCCAGGCTTCAAAGACGACCt                                                                                                                                      <  2:379460/151‑1 (MQ=255)

            gTATCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGTTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAGATAACGATTAATATCGCGCAGCAACGCCTGCGGCCCTTTCCAGGCTTCAAAGACGACCt                                                                                                                                      <  1:244344/151‑1 (MQ=255)

              aTCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGTTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAGATAACGATTAATATCGCGCAGCAACGCCTGCGGCCCTTTCCAGGCTTCAAAGACGACCTgg                                                                                                                                    <  2:712539/151‑1 (MQ=255)

                 aaTACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGTTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAGATAACGATTAATATCGCGCAGCAACGCCTGCGGCCCTTTCCAGGCTTCAAAGACGACCTGGGCt                                                                                                                                 >  1:152973/1‑151 (MQ=255)

                    aCGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGTTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAGATAACGATTAATATCGCGCAGCAACGCCTGCGGCCCTTTCCAGGCTTCAAAGACGACCTGGGCTAtt                                                                                                                              >  2:812146/1‑151 (MQ=255)

                    aCGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGTTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAGATAACGATTAATATCGCGCAGCAACGCCTGCGGCCCTTTCCAGGCTTCAAAGACGACCTGGGCTAt                                                                                                                               <  2:198870/150‑1 (MQ=255)

                               tttGCGCGTGACGGGAAGCCAGCGTTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAGATAACGATTAATATCGCGCAGCAACGCCTGCGGCCCTTTCCAGGCTTCAAAGACGACCTGGGCTATTTCACGCGGc                                                                                                                     >  2:203392/1‑149 (MQ=255)

                                      gTGACGGGAAGCCAGCGTTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAGATAACGATTAATATCGCGCAGCAACGCCTGCGGCCCTTTCCAGGCTTCAAAGACGACCTGGGCTATTTCACGCGGCAGCGGGTAg                                                                                                            <  1:526153/151‑1 (MQ=255)

                                        gACGGGAAGCCAGCGTTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAGATAACGATTAATATCGCGCAGCAACGCCTGCGGCCCTTTCCAGGCTTCAAAGACGACCTGGGCTATTTCACGCGGCAGCGGGTAGc                                                                                                           >  1:857081/1‑150 (MQ=255)

                                          cGGGAAGCCAGCGTTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAGATAACGATTAATATCGCGCAGCAACGCCTGCGGCCCTTTCCAGGCTTCAAAGACGACCTGGGCTATTTCACGCGGCAGCGGGTAGCAGt                                                                                                        <  1:203369/151‑1 (MQ=255)

                                                    gCGTTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAGATAACGATTAATATCGCGCAGCAACGCCTGCGGCCCTTTCCAGGCTTCAAAGACGACCTGGGCTATTTCACGCGGCAGCGGGTAGCAGTGGCGACGCCa                                                                                              <  1:305338/151‑1 (MQ=255)

                                                       tttCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAGATAACGATTAATATCGCGCAGCAACGCCTGCGGCCCTTTCCAGGCTTCAAAGACGACCTGGGCTATTTCACGCGGCAGCGGGTAGCAGTGGCGACGCCAGa                                                                                            >  1:26351/1‑150 (MQ=255)

                                                        ttCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAGATAACGATTAATATCGCGCAGCAACGCCTGCGGCCCTTTCCAGGCTTCAAAGACGACCTGGGCTAtt                                                                                                                              <  2:737914/115‑1 (MQ=255)

                                                        ttCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAGATAACGATTAATATCGCGCAGCAACGCCTGCGGCCCTTTCCAGGCTTCAAAGACGACCTGGGCTAtt                                                                                                                              >  1:737871/1‑115 (MQ=255)

                                                                 gggCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAGATAACGATTAATATCGCGCAGCAACGCCTGCGGCCCTTTCCAGGCTTCAAAGACGACCTGGGCTATTTCACGCGGCAGCGGGTAGCAGTGGCGACGCCAGAAATCGGCGCAg                                                                                 <  1:283210/151‑1 (MQ=255)

                                                                 gggCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAGATAACGATTAATATCGCGCAGCAACGCCTGCGGCCCTTTCCAGGCTTCAAAGACGACCTGGGCTATTTCACGCGGCAGCGGGTAGCAGTGGCGACGCCAGAAATCGGCGCAg                                                                                 <  2:663342/151‑1 (MQ=255)

                                                                 gggCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAGATAACGATTAATATCGCGCAGCAACGCCTGCGGCCCTTTCCAGGCTTCAAAGACGACCTGGGCTATTTCACGCGGCAGCGGGTAGCAGTGGCGACGCCAGAAATCGGCGCAg                                                                                 <  2:369941/151‑1 (MQ=255)

                                                                              ttGATAACCGGCGCTTCGCCTTGCAGATAACGATTAATATCGCGCAGCAACGCCTGCGGCCCTTTCCAGGCTTCAAAGACGACCTGGGCTATTTCACGCGGCAGCGGGTAGCAGTGGCGACGCCAGAAATCGGCGCAGGCCTGgtagcg                                                                      <  2:689980/149‑1 (MQ=255)

                                                                               tGATAACCGGCGCTTCGCCTTGCAGATAACGATTAATATCGCGCAGCAACGCCTGCGGCCCTTTCCAGGCTTCAAAGACGACCTGGGCTATTTCACGCGGCAGCGGGTAGCAGTGGCGACGCCAGAAATCGGCGCAGGCCTGgtagcgt                                                                     >  1:228838/1‑149 (MQ=255)

                                                                                 aTAACCGGCGCTTCGCCTTGCAGATAACGATTAATATCGCGCAGCAACGCCTGCGGCCCTTTCCAGGCTTCAAAGACGACCTGGGCTATTTCACGCGGCAGCGGGTAGCAGTGGCGACGCCAGAAATCGGCGCAGGCCTGgtagcgtagc                                                                  >  1:129556/1‑150 (MQ=255)

                                                                                         cgcTTCGCCTTGCAGATAACGATTAATATCGCGCAGCAACGCCTGCGGCCCTTTCCAGGCTTCAAAGACGACCTGGGCTATTTCACGCGGCAGCGGGTAGCAGTGGCGACGCCAGAAATCGGCGCAGGCCTGGTAGCGTAGCAGAGACTc                                                          >  1:417047/1‑150 (MQ=255)

                                                                                         cgcTTCGCCTTGCAGATAACGATTAATATCGCGCAGCAACGCCTGCGGCCCTTTCCAGGCTTCAAAGACGACCTGGGCTATTTCACGCGGCAGCGGGTAGCAGTGGCGACGCCAGAAATCGGCGCAGGCCTGGTAGCGTAGCAGAGACTCa                                                         <  2:805206/151‑1 (MQ=255)

                                                                                              cGCCTTGCAGATAACGATTAATATCGCGCAGCAACGCCTGCGGCCCTTTCCAGGCTTCAAAGACGACCTGGGCTATTTCACGCGGCAGCGGGTAGCAGTGGCGACGCCAGAAATCGGCGCAGGCCTGGTAGCGTAGCAGAGACTCATCtt                                                     <  2:334646/150‑1 (MQ=255)

                                                                                                   tGCAGATAACGATTAATATCGCGCAGCAACGCCTGCGGCCCTTTCCAGGCTTCAAAGACGACCTGGGCTATTTCACGCGGCAGCGGGTAGCAGTGGCGACGCCAGAAATCGGCGCAGGCCTGGTAGCGTAGCAGAGACTCATCTTCAATc                                                <  2:706527/150‑1 (MQ=255)

                                                                                                    gCAGATAACGATTAATATCGCGCAGCAACGCCTGCGGCCCTTTCCAGGCTTCAAAGACGACCTGGGCTATTTCACGCGGCAGCGGGTAGCAGTGGCGACGCCAGAAATCGGCGCAGGCCTGGTAGCGTAGCAGAGACTCATCTTCAATCAg                                              <  2:633823/151‑1 (MQ=255)

                                                                                                      aGATAACGATTAATATCGCGCAGCAACGCCTGCGGCGCTTTCCAGGCTTCAAAGACGACCTGGGCTATTTCACGCGGCAGCGGGTAGCAGTGGCGACGCCAGAAATCGGCGCAGGCCTGGTAGCGTAGCAGAGACTCATCTTCAATCAgct                                            >  2:575618/1‑151 (MQ=255)

                                                                                                          aaCGATTAATATCGCGCAGCAACGCCTGCGGCCCTTTCCAGGCTTCAAAGACGACCTGGGCTATTTCACGCGGCAGCGGGTAGCAGTGGCGACGCCAGAAATCGGCGCAGGCCTGGTAGCGTAGCAGAGACTCATCTTCAATCAgctgct                                         <  2:792558/150‑1 (MQ=255)

                                                                                                          aaCGATTAATATCGCGCAGCAACGCCTGCGGCCCTTTCCAGGCTTCAAAGACGACCTGGGCTATTTCACGCGGCAGCGGGTAGCAGTGGCGACGCCAGAAATCGGCGCAGGCCTGGAAGCGTAGCAGAGACTCATCTTCAATCAgctgctg                                        >  1:382557/1‑151 (MQ=255)

                                                                                                           aCGATTAATATCGCGCAGCAACGCCTGCGGCCCTTTCCAGGCTTCAAAGACGACCTGGGCTATTTCACGCGGCAGCGGGTAGCAGTGGCGACGCCAGAAATCGGCGCAGGCCTGGTAGCGTAGCAGAGACTCATCTTCAATCAgctgctgc                                       <  1:779381/151‑1 (MQ=255)

                                                                                                             gATTAATATCGCGCAGCAACGCCTGCGGCCCTTTCCAGGCTTCAAAGACGACCTGGGCTATTTCACGCGGCAGCGGGTAGCAGTGGCGACGCCAGAAATCGGCGCAGGCCTGGTAGCGTAGCAGAGACTCATCTTCAATCAGCTGCTGCTc                                     <  1:442368/151‑1 (MQ=255)

                                                                                                              aTTAATATCGCGCAGCAACGCCTGCGGCCCTTTCCAGGCTTCAAAGACGACCTGGGCTATTTCACGCGGCAGCGGGTAGCAGTGGCGACGCCAGAAATCGGCGCAGGCCTGGTAGCGTAGCAGAGACTCATCTTCAATCAGCTGCTGCTCa                                    <  2:661203/151‑1 (MQ=255)

                                                                                                              aTTAATATCGCGCAGCAACGCCTGCGGCCCTTTCCAGGCTTCAAAGACGACCTGGGCTATTTCACGCGGCAGCGGGTAGCAGTGGCGACGCCAGAAATCGGCGCAGGCCTGGTAGCGTAGCAGAGACTCATCTTCAATCAGCTGCTGCTCa                                    <  1:724042/151‑1 (MQ=255)

                                                                                                                tAATATCGCGCAGCAACGCCTGCGGCCCTTTCCAGGCTTCAAAGACGACCTGGGCTATTTCACGCGGCAGCGGGTAGCAGTGGCGACGCCAGAAATCGGCGCAGGCCTGGTAGCGTAGCAGAGACTCATCTTCAATCAGCTGCTGCTCaa                                   >  2:795906/1‑150 (MQ=255)

                                                                                                                 aaTATCGCGCAGCAACGCCTGCGGCCCTTTCCAGGCTTCAAAGACGACCTGGGCTATTTCACGCGGCAGCGGGTAGCAGTGGCGACGCCAGAAATCGGCGCAGGCCTGGTAGCGTAGCAGAGACTCATCTTCAATCAGCTGCTGCTCaaa                                  <  1:548196/150‑1 (MQ=255)

                                                                                                                 aaTATCGCGCAGCAACGCCTGCGGCCCTTTCCAGGCTTCAAAGACGACCTGGGCTATTTCACGCGGCAGCGGGTAGCAGTGGCGACGCCAGAAATCGGCGCAGGCCTGGTAGCGTAGCAGAGACTCATCTTCAATCAGCTGCTGCTCAAAc                                 <  2:407761/151‑1 (MQ=255)

                                                                                                                  atatCGCGCAGCAACGCCTGCGGCCCTTTCCAGGCTTCAAAGACGACCTGGGCTATTTCACGCGGCAGCGGGTAGCAGTGGCGACGCCAGAAATCGGCGCAGGCCTGGTAGCGTAGCAGAGACTCATCTTCAATCAGCTGCTGCTCaaa                                  <  2:459232/149‑1 (MQ=255)

                                                                                                                    atCGCGCAGCAACGCCTGCGGCCCTTTCCAGGCTTCAAAGACGACCTGGGCTATTTCACGCGGCAGCGGGTAGCAGTGGCGACGCCAGAAATCGGCGCAGGCCTGGTAGCGTAGCAGAGACTCATCTTCAATCAGCTGCTGCTCAAACAGc                              >  2:617545/1‑151 (MQ=255)

                                                                                                                       gcgcAGCAACGCCTGCGGCCCTTTCCAGGCTTCAAAGACGACCTGGGCTATTTCACGCGGCAGCGGGTAGCAGTGGCGACGCCAGAAATCGGCGCAGGCCTGGTAGCGTAGCAGAGACTCATCTTCAATCAGCTGCTGCTCAAACAGCATg                           >  2:106923/1‑151 (MQ=255)

                                                                                                                         gcagcaACGCCTGCGGCCCTTTCCAGGCTTCAAAGACGACCTGGGCTATTTCACGCGGCAGCGGGTAGCAGTGGCGACGCCAGAAATCGGCGCAGGCCTGGTAGCGTAGCAGAGACTCATCTTCAATCAGCTGCTGCTCAAACAGCATGcc                         >  1:668283/1‑151 (MQ=255)

                                                                                                                          cagcaACGCCTGCGGCCCTTTCCAGGCTTCAAAGACGACCTGGGCTATTTCACGCGGCAGCGGGTAGCAGTGGCGACGCCAGAAATCGGCGCAGGCCTGGTAGCGTAGCAGAGACTCATCTTCAATCAGCTGCTGCTCAAACAGCATGCCg                        >  2:564226/1‑151 (MQ=255)

                                                                                                                             caACGCCTGCGGCCCTTTCCAGGCTTCAAAGACGACCTGGGCTATTTCACGCGGCAGCGGGTAGCAGTGGCGACGCCAGAAATCGGCGCAGGCCTGGTAGCGTAGCAGAGACTCATCTTCAATCAGCTGCTGCTCAAACAGCATGCCGGAt                     <  2:448891/151‑1 (MQ=255)

                                                                                                                                    tGCGGCCCTTTCCAGGCTTCAAAGACGACCTGGGCTATTTCACGCGGCAGCGGGTAGCAGTGGCGACGCCAGAAATCGGCGCAGGCCTGGTAGCGTAGCAGAGACTCATCTTCAATCAGCTGCTGCTCAAACAGCATGCCGGATTCAAAgg              >  1:706178/1‑151 (MQ=255)

                                                                                                                                        gCCCTTTCCAGGCTTCAAAGACGACCTGGGCTATTTCACGCGGCAGCGGGTAGCAGTGGCGACGCCAGAAATCGGCGCAGGCCTGGTAGCGTAGCAGAGACTCATCTTCAATCAGCTGCTGCTCAAACAGCATGCCGGATTCAAAGGCAtt          <  1:637244/151‑1 (MQ=255)

                                                                                                                                          ccTTTCCAGGCTTCAAAGTCGACCTGGGCTATTTCACGCGGCAGCGGGTAGCAGTGGCGACGCCAGAAATCGGCGCAGGCCTGGTAGCGTAGCAGAGACTCATCTTCAATCAGCTGCTGCTCAAACAGCATGCCGGATTCAAAGGCATTCa        <  1:235206/151‑1 (MQ=255)

                                                                                                                                           cTTTCCAGGCTTCAAAGACGACCTGGGCTATTTCACGCGGCAGCGGGTAGCAGTGGCGACGCCAGAAATCGGCGCAGGCCTGGTAGCGTAGCAGAGACTCATCTTCAATCAGCTGCTGCTCAAACAGCATGCCGGATTCAAAGGCATTc         >  2:705444/1‑149 (MQ=255)

                                                                                                                                             ttCCAGGCTTCAAAGACGACCTTGGCTATTTCACGCGGCAGCGGGGATCAGTGGCGACGCCAGAAATCGGCGCAGGCCTGGTAGCGTAGCAGAGACTCATCTTCAATCAGCTGCTGCTCAAACAGCATGCTGGATTCAAAGGCATTCAGGt     >  1:460999/1‑151 (MQ=255)

                                                                                                                                                cAGGCTTCAAAGACGACCTGGGCTATTTCACGCGGCAGCGGGTAGCAGTGGCGACGCCAGAAATCGGCGCAGGCCTGGTAGCGTAGCAGAGACTCATCTTCAATCAGCTGCTGCTCAAACAGCATGCCGGATTCAAAGGCATTCAGGTTGa  <  1:811505/151‑1 (MQ=255)

TGCTGTTTTACCGTATCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGTTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAGATAACGATTAATATCGCGCAGCAACGCCTGCGGCCCTTTCCAGGTTTCAAAGACGACCTGGGCTATTTCACGCGGCAGCGGGTAGCAGTGGCGACGCCAGAAATCGGCGCAGGCCTGGTAGCGTAGCAGAGACTCATCTTCAATCAGCTGCTGCTCAAACAGCATGCCGGATTCAAAGGCATTCAGGTTGA  >  NC_000913/2955300‑2955594

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A