Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A3 F50 I1 R1	300	54.7	1718999	98.4%	1691495	149.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,989,983	A→G	A126A (GCT→GCC)	yqeK ←	uncharacterized protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,989,983	0	A	G	100.0%	189.2 / NA	52	A126A (GCT→GCC)	yqeK	uncharacterized protein
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (22/30); total (22/30)

AAAATTAAATATTCTCGCAGTATATGGCAGTCTAAAGCATCAAAGATTTGATCAACATCTTTCATTTTAGACATCTCCTTGCAATGTAAAATATCATGTATCAACAACATCTGGTGCAAATCCATGAGTCGAACTCGACATTCATCTTAGCTCGATTATTATTCCTTCGTACAGTCGATGTAAACAATACAGAAAGAGGATTATTAAGAACCAGTTTACTCAGTGAATCAATAGAGGAAAGGACTAACGTTTCTTTAAAAGAATTGATTTCATCATCTGTTAAACTAAACTCATC  >  NC_000913/2989835‑2990129

aaaaTTAAATATTCTCGCAGTATATGGCAGTCTAAAGCATCAAAGATTTGATCAACATCTTTCATTTTAGACATCTCCTTGCAATGTAAAATATCATGTATCAACAACATCTGGTGCAAATCCATGAGTCGAACTCGACATTCATCTTGGc                                                                                                                                                  >  1:734625/1‑151 (MQ=255)

  aaTTAAATATTCTCGCAGTATATGGCAGTCTAAAGCATCAAAGATTTGATCAACATCTTTCATTTTAGACATCTCCTTGCAATGTAAAATATCATGTATCAACAACATCTGGTGCAAATCCATGAGTCGAACTCGACATTCATCTTGGCTc                                                                                                                                                >  2:61514/1‑151 (MQ=255)

       aaTATTCTCGCAGTATATGGCAGTCTAAAGCATCAAAGATTTGATCAACATCTTTCATTTTAGACATCTCCTTGCAATGTAAAATATCATGTATCAACAACATCTGGTGCAAATCCATGAGTCGAACTCGACATTCATCTTGGCTCGatta                                                                                                                                           <  1:114303/151‑1 (MQ=255)

       aaTATTCTCGCAGTATATGGCAGTCTAAAGCATCAAAGATTTGATCAACATCTTTCATTTTAGACATCTCCTTGCAATGTAAAATATCATGTATCAACAACATCTGGTGCAAATCCATGAGTCGAACTCGACATTCATCTTGGCTCGatta                                                                                                                                           <  2:595290/151‑1 (MQ=255)

        atatTCTCGCAGTATATGGCAGTCTAAAGCATCAAAGATTTGATCAACATCTTTCATTTTAGACATCTCCTTGCAATGTAAAATATCATGTATCAACAACATCTGGTGCAAATCCATGAGTCGAACTCGACATTCATCTTGGCTCGattat                                                                                                                                          <  2:73691/151‑1 (MQ=255)

          atTCTCGCAGTATATGGCAGTCTAAAGCATCAAAGATCTGATCAACATCTTTCATTTTAGACATCTCCTTGCAATGTAAAATATCATGTATCAACAACATCTGGTGCAAATCCATGAGTCGAACTCGACTTTCATCTTGGCTCGattatt                                                                                                                                         <  1:520199/150‑1 (MQ=255)

               cGCAGTATATGGCAGTCTAAAGCATCAAAGATTTGATCAACATCTTTCATTTTAGACATCTCCTTGCAATGTAAAATATCATGTATCAACAACATCTGGTGCAAATCCATGAGTCGAACTCGACATTCATCTTGGCTCGATTATTATTc                                                                                                                                     <  1:843831/149‑1 (MQ=255)

               cGCAGTATATGGCAGTCTAAAGCATCAAAGATTTGATCAACATCTTTCATTTTAGACATCTCCTTGCAATGTAAAATATCATGTATCAACAACATCTGGTGCAAATCCATGAGTCGAACTCGACATTCATCTTGGCTCGATTATTATTCCt                                                                                                                                   >  2:437406/1‑151 (MQ=255)

                         ggCAGTCTAAAGCATCAAAGATTTGATCAACATCTTTCATTTTAGACATCTCCTTGCAATGTAAAATATCATGTATCAACAACATCTGGTGCAAATCCATGAGTCGAACTCGACATTCATCTTGGCTCGATTATTATTCCTTCGTACAGt                                                                                                                          <  2:828694/150‑1 (MQ=255)

                         ggCAGTCTAAAGCATCAAAGATTTGATCAACATCTTTCATTTTAGACATCTCCTTGCAATGTAAAATATCATGTATCAACAACATCTGGTGCAAATCCATGAGTCGAACTCGACATTCATCTTGGCTCGATTATTATTCCTTCGTACAGt                                                                                                                          <  2:709356/150‑1 (MQ=255)

                         ggCAGTCTAAAGCATCAAAGATTTGATCAACATCTTTCATTTTAGACATCTCCTTGCAATGTAAAATATCATGTATCAACAACATCTGGTGCAAATCCATGAGTCGAACTCGACATTCATCTTGGCTCGATTATTATTCCTTCGTACAGTc                                                                                                                         <  1:716365/151‑1 (MQ=255)

                            aGTCTAAAGCATCAAAGATTTGATCAACATCTTTCATTTTAGACATCTCCTTGCAATGTAAAATATCATGTATCAACAACATCTGGTGCAAATCCATGAGTCGAACTCGACATTCATCTTGGCTCGATTATTATTCCTTCGTACAGTCGAt                                                                                                                      >  2:538159/1‑151 (MQ=255)

                              tCTAAATCATCAAAGATTCGATCAACATCTTTCATTTTAGACATCTCCTGGCAATGTAAAATATCATGTATCAACAACATCTGGTGCAAATCCATGAGTCGAACTCGACATTCATCTTGGCTCGAGTATTATTCCTTCGGACAGTCGTTg                                                                                                                     <  1:17278/150‑1 (MQ=255)

                                   aGCATCAAAGATTTGATCAACATCTTTCATTTTAGACATCTCCTTGCAATGTAAAATATCATGTATCAACAACATCTGGTGCAAATCCATGAGTCGAACTCGACATTCATCTTGGCTCGATTATTATTCCTTCGTACAGTCGATGTAAAc                                                                                                                >  2:548978/1‑150 (MQ=255)

                                        cAAAGATTTGATCAACATCTTTCATTTTAGACATCTCCTTGCAATGTAAAATATCATGTATCAACAACATCTGGTGCAAATCCATGAGTCGAACTCGACATTCATCTTGGCTCGATTATTATTCCTTCGTACAGTCGATGTAAACAATACa                                                                                                          <  1:319327/151‑1 (MQ=255)

                                        cAAAGATTTGATCAACATCTTTCATTTTAGACATCTCCTTGCAATGTAAAATATCATGTATCAACAACATCTGGTGCAAATCCATGAGTCGAACTCGACATTCATCTTGGCTCGATTATTATTCCTTCGTACAGTCGATGTAAACAATACa                                                                                                          <  2:613270/151‑1 (MQ=255)

                                          aaGATTTGATCAACATCTTTCATTTTAGACATCTCCTTGCAATGTAAAATATCATGTATCAACAACATCTGGTGCAAATCCATGAGTCGAACTCGACATTCATCTTGGCTCGATTATTATTCCTTCGTACAGTCGATGTAAACAATACAGa                                                                                                        <  1:496542/151‑1 (MQ=255)

                                            gATTTGATCAACATCTTTCATTTTAGACATCTCCTTGCAATGTAAAATATCATGTATCAACAACATCTGGTGCAAATCCATGAGTCGAACTCGACATTCATCTTGGCTCGATTATTATTCCTTCGTACAGTCGATGTAAACAATACAGaaa                                                                                                      <  2:454948/151‑1 (MQ=255)

                                                tGATCAACATCTTTCATTTTAGACATCTCCTTGCAATGTAAAATATCATGTATCAACAACATCTGGTGCAAATCCATGAGTCGAACTCGACATTCATCTTGGCTCGATTATTATTCCTTCGTACAGTCGATGTAAACAATACAGAAagag                                                                                                   <  1:393507/150‑1 (MQ=255)

                                                tGATCAACATCTTTCATTTTAGACATCTCCTTGCAATGTAAAATATCATGTATCAACAACATCTGGTGCAAATCCATGAGTCGAACTCGACATTCATCTTGGCTCGATTATTATTCCTTCGTACAGTCGATGTAAACAATACAGAAagag                                                                                                   <  2:503930/150‑1 (MQ=255)

                                                 gATCAACATCTTTCATTTTAGACATCTCCTTGCAATGTAAAATATCATGTATCAACAACATCTGGTGCAAATCCATGAGTCGAACTCGACATTCATCTTGGCTCGATTATTATTCCTTCGTACAGTCGATGTAAACAATACAGAAAGAgg                                                                                                  >  2:188204/1‑150 (MQ=255)

                                                 gATCAACATCTTTCATTTTAGACATCTCCTTGCAATGTAAAATATCATGTATCAACAACATCTGGTGCAAATCCATGAGTCGAACTCGACATTCATCTTGGCTCGATTATTATTCCTTCGTACAGTCGATGTAAACAATACAGAAAGAgg                                                                                                  >  2:392784/1‑150 (MQ=255)

                                                       cATCTTTCATTTTAGACATCTCCTTGCAATGTAAAATATCATGTATCAACAACATCTGGTGCAAATCCATGAGTCGAACTCGACATTCATCTTGGCTCGATTATTATTCCTTCGTACAGTCGATGTAAACAATACAGAAAGAGGattatt                                                                                            >  1:197881/1‑150 (MQ=255)

                                                        aTCTTTCATTTTAGACATCTCCTTGCAATGTAAAATATCATGTATCAACAACATCTGGTGCAAATCCATGAGTCGAACTCGACATTCATCTTGGCTCGATTATTATTCCTTCGTACAGTCGATGTAAACAATACAGAAAGAGGAttatta                                                                                           >  2:588397/1‑150 (MQ=255)

                                                         tCTTTCATTTTAGACATCTCCTTGCAATGTAAAATATCATGTATCAACAACATCTGGTGCAAATCCATGAGTCGAACTCGACATTCATCTTGGCTCGATTATTATTCCTTCGTACAGTCGATGTAAACAATACAGAAAGAGGAttatta                                                                                           >  1:410637/1‑149 (MQ=255)

                                                          cTTTCATTTTAGACATCTCCTTGCAATGTAAAATATCATGTATCAACAACATCTGGTGCAAATCCATGAGTCGAACTCGACATTCATCTTGGCTCGATTATTATTCCTTCGTACAGTCGATGTAAACAATACAGAAAGAGGAttatta                                                                                           >  2:226294/1‑148 (MQ=255)

                                                              cATTTTAGACATCTCCTTGCAATGTAAAATATCATGTATCAACAACATCTGGTGCAAATCCATGAGTCGAACTCGACATTCATCTTGGCTCGATTATTATTCCTTCGTACAGTCGATGTAAACAATACAGAAAGAGGATTATTAAGAACCa                                                                                    <  1:519454/151‑1 (MQ=255)

                                                                   tAGACATCTCCTTGCAATGTAAAATATCATGTATCAACAACATCTGGTGCAAATCCATGAGTCGAACTCGACATTCATCTTGGCTCGATTATTATTCCTTCGTACAGTCGATGTAAACAATACAGAAAGAGGATTATTAAGAACCAGttt                                                                                >  2:718462/1‑150 (MQ=255)

                                                                         tctcCTTGCAATGTAAAATATCATGTATCAACAACATCTGGTGCAAATCCATGAGTCGAACTCGACATTCATCTTGGCTCGATTATTATTCCTTCGTACAGTCGATGTAAACAATACAGAAAGAGGATTATTAAGAACCAGTTTACTCAg                                                                          >  1:248243/1‑150 (MQ=255)

                                                                               tGCAATGTAAAATATCATGTATCAACAACATCTGGTGCAAATCCATGAGTCGAACTCGACATTCATCTTGGCTCGATTATTATTCCTTCGTACAGTCGATGTAAACAATACAGAAAGAGGATTATTAAGAACCAGTTTACTCAGTGAAt                                                                     <  1:809268/149‑1 (MQ=255)

                                                                                 cAATGTAAAATATCATGTATCAACAACATCTGGTGCAAATCCATGAGTCGAACTCGACATTCATCTTGGCTCGATTATTATTCCTTCGTACAGTCGATGTAAACAATACAGAAAGAGGATTATTAAGAACCAGTTTACTCAGTGAATCaa                                                                  <  2:626278/150‑1 (MQ=255)

                                                                                   aTGTAAAATATCATGTATCAACAACATCTGGTGCAAATCCATGAGTCGAACTCGACATTCATCTTGGCTCGATTATTATTCCTTCGTACAGTCGATGTAAACAATACAGAAAGAGGATTATTAAGAACCAGTTTACTCAGTGAATCAATag                                                               <  1:72553/151‑1 (MQ=255)

                                                                                     gTAAAATATCATGTATCAACAACATCTGGTGCAAATCCATGAGTCGAACTCGACATTCATCTTGGCTCGATTATTATTCCTTCGTACAGTCGATGTAAACAATACAGAAAGAGGATTATTAAGAACCAGTTTACTCAGTGAATCAATagag                                                             <  1:165066/151‑1 (MQ=255)

                                                                                     gTAAAATATCATGTATCAACAACATCTGGTGCAAATCCATGAGTCGAACTCGACATTCATCTTGGCTCGATTATTATTCCTTCGTACAGTCGATGTAAACAATACAGAAAGAGGATTATTAAGAACCAGTTTACTCAGTGAATCAATaga                                                              <  2:531254/150‑1 (MQ=255)

                                                                                      tAAAATATCATGTATCAACAACATCTGGTGCAAATCCATGAGTCGAACTCGACATTCATCTTGGCTCGATTATTATTCCTTCGTACAGTCGATGTAAACAATACAGAAAGAGGATTATTAAGAACCAGTTTACTCAGTGAATCAATAGAgg                                                            >  2:240446/1‑151 (MQ=255)

                                                                                        aaaTATCATGTATCAACAACATCTGGTGCAAATCCATGAGTCGAACTCGACATTCATCTTGGCTCGATTATTATTCCTTCGTACAGTCGATGTAAACAATAc                                                                                                           <  2:229851/102‑1 (MQ=255)

                                                                                        aaaTATCATGTATCAACAACATCTGGTGCAAATCCATGAGTCGAACTCGACATTCATCTTGGCTCGATTATTATTCCTTCGTACAGTCGATGTAAACAATAc                                                                                                           >  1:229827/1‑102 (MQ=255)

                                                                                         aaTATCATGTATCAACAACATCTGGTGCAAATCCATGAGTCGAACTCGACATTCATCTTGGCTCGATTATTATTCCTTCGTACAGTCGATGTAAACAATACAGAAAGAGGATTATTAAGAACCAGTTTACTCAGTGAATCAATAGAGGaa                                                          >  1:125566/1‑150 (MQ=255)

                                                                                          atatCATGTATCAACAACATCTGGTGCAAATCCATGAGTCGAACTCGACATTCATCTTGGCTCGATTATTATTCCTTCGTACAGTCGATGTAAACAATACAGGAAGAGGATTCTTAAGAACCAGTTTACTCAGTGAATCAATAGCGGaa                                                          >  2:527654/1‑149 (MQ=255)

                                                                                                 gTATCAACAACATCTGGTGCAAATCCATGAGTCGAACTCGACATTCATCTTGGCTCGATTATTATTCCTTCGTACAGTCGATGTAAACAATACAGAAAGAGGATTATTAAGAACCAGTTTACTCAGTGAATCAATAGAGGAAAGGACTaa                                                  <  2:191373/150‑1 (MQ=255)

                                                                                                        caacaTCTGGTGCAAATCCATGAGTCGAACTCGACATTCATCTTGGCTCGATTATTATTCCTTCGTACAGTCGATGTAAACAATACAGAAAGAGGATTATTAAGAACCAGTTTACTCAGTGAATCAATAGAGGAAAGGACTAACGTTTCtt                                          <  1:629950/151‑1 (MQ=255)

                                                                                                         aacaTCTGGTGCAAATCCATGAGTCGAACTCGACATTCATCTTGGCTCGATTATTATTCCTTCGTACAGTCGATGTAAACAATACAGAAAGAGGATTATTAAGAACCAGTTTACTCAGTGAATCAATAGAGGAAAGGACTAACGTTTCttt                                         <  1:706177/151‑1 (MQ=255)

                                                                                                              cTGGTGCAAATCCATGAGTCGAACTCGACATTCATCTTGGCTCGATTATTATTCCTTCGTACAGTCGATGTAAACAATACAGAAAGAGGATTATTAAGAACCAGTTTACTCAGTGAATCAATAGAGGAAAGGACTAACGTTTCTTTaaa                                      >  1:537052/1‑149 (MQ=255)

                                                                                                                ggTGCAAATCCATGAGTCGAACTCGACATTCATCTTGGCTCGATTATTATTCCTTCGTACAGTCGATGTAAACAATACAGAAAGAGGATTATTAAGAACCAGTTTACTCAGTGAATCAATAGAGGAAAGGACTAACGTTTCTTTaaa                                      <  1:771232/147‑1 (MQ=255)

                                                                                                                ggTGCAAATCCATGAGTCGAACTCGACATTCATCTTGGCTCGATTATTATTCCTTCGTACAGTCGATGTAAACAATACAGAAAGAGGATTATTAAGAACCAGTTTACTCAGTGAATCAATAGAGGAAAGGACTAACGTTTCTTTAAAAGaa                                  <  1:540542/151‑1 (MQ=255)

                                                                                                                          cATGAGTCGAACTCGACATTCATCTTGGCTCGATTATTATTCCTTCGTACAGTCGATGTAAACAATACAGAAAGAGGATTATTAAGAACCAGTTTACTCAGTGAATCAATAGAGGAAAGGACTAACGTTTCTTTAAAAGAATTGATTtcat                        <  1:429387/151‑1 (MQ=255)

                                                                                                                              aGTCGAACTCGACATTCATCTTGGCTCGATTATTATTCCTTCGTACAGTCGATGTAAACAATACAGAAAGAGGATTATTAAGAACCAGTTTACTCAGTGAATCAATAGAGGAAAGGACTAACGTTTCTTTAAAAGAATTGATTTcatcat                     >  1:284843/1‑150 (MQ=255)

                                                                                                                                       cGACATTCATCTTGGCTCGATTATTATTCCTTCGTACAGTCGATGTAAACAATACAGAAAGAGGATTATTAAGAACCAGTTTACTCAGTGAATCAATAGAGGAAAGGACTAACGTTTCTTTAAAAGAATTGATTTCATCATCTGTtaaac            <  1:511551/150‑1 (MQ=255)

                                                                                                                                          cATTCATCTTGGCTCGATTATTATTCCTTCGTACAGTCGATGTAAACAATACAGAAAGAGGATTATTAAGAACCAGTTTACTCAGTGAATCAATAGAGGAAAGGACTAACGTTTCTTTAAAAGAATTGATTTCATCATCTGTtaaactaa         >  2:816038/1‑150 (MQ=255)

                                                                                                                                           aTTCATCTTGGCTCGATTATTATTCCTTCGTATAGTCGATGTAAACAATACAGAAAGAGGATTATTAAGAACCAGTTTACTCAGTGAATCAATAGAGGAAAGGACTAACGTTTCTTTAAAAGAATTGATTTCATCATCTGTtaaactaaac       <  2:192899/151‑1 (MQ=255)

                                                                                                                                                tCTTGGCTCGATTATTATTCCTTCGTACAGTCGATGTAAACAATACAGAAAGAGGATTATTAAGAACCAGTTTACTCAGTGAATCAATAGAGGAAAGGACTAACGTTTCTTTAAAAGAATTGATTTCATCATCTGTTAAACTAAACtcatc  >  1:615322/1‑151 (MQ=255)

                                                                                                                                                 cTTGGCTCGATTATTATTCCTTCGTACAGTCGATGTAAACAATACAGAAAGAGGATTATTAAGAACCAGTTTACTCAGTGAATCAATAGAGGAAAGGACTAACGTTTCTTTAAAAGAATTGATTTCATCATCTGTTAAACTAAACtcatc  >  1:566668/1‑150 (MQ=255)

AAAATTAAATATTCTCGCAGTATATGGCAGTCTAAAGCATCAAAGATTTGATCAACATCTTTCATTTTAGACATCTCCTTGCAATGTAAAATATCATGTATCAACAACATCTGGTGCAAATCCATGAGTCGAACTCGACATTCATCTTAGCTCGATTATTATTCCTTCGTACAGTCGATGTAAACAATACAGAAAGAGGATTATTAAGAACCAGTTTACTCAGTGAATCAATAGAGGAAAGGACTAACGTTTCTTTAAAAGAATTGATTTCATCATCTGTTAAACTAAACTCATC  >  NC_000913/2989835‑2990129

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A