Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A3 F50 I1 R1	300	54.7	1718999	98.4%	1691495	149.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,019,115	T→C	V1019A (GTC→GCC)	ygfK →	putative Fe‑S subunit oxidoreductase subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,019,115	0	T	C	100.0%	152.5 / NA	42	V1019A (GTC→GCC)	ygfK	putative Fe‑S subunit oxidoreductase subunit
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (24/18); total (24/18)

ACAGCAGCAACCCAGGCTTCCTTGTGGAAGATTGCCGGGTACGAGTACGTCTGAATAACCAAAGCTGGGTGTTAAACATCGACAGCAAAGGTCAGTTTAACAACGTACCACCGGAGCTGAACGATATGTGCCGCATCATCAGCCATGTCCACCAGCATCATCATTATCTGCTGGGCCGCGTGGAGGTGTAATCATGTTGATTCTGAAGAATGTCACTGCGGTACAGCTACACCCGGCAAAAGTGCAGGAAGGCGTTGATATCGCCATCGAAAACGATGTGATTGTCGCTATCGG  >  NC_000913/3018968‑3019261

aCAGCAGCAACCCAGGCTTCCTTGTGGAAGATTGCCGGGTACGAGTACGTCTGAATAACCAAAGCTGGGTGTTAAACATCGACAGCAAAGGTCAGTTTAACAACGTACCACCGGAGCTGAACGATATGTGCCGCATCATCAGCCATGccc                                                                                                                                                  >  1:203811/1‑150 (MQ=255)

aCAGCAGCAACCCAGGCTTCCTTGTGGAAGATTGCCGGGTACGAGTACGTCTGAATAACCAAAGCTGGGTGTTAAACATCGACAGCAAAGGTCAGTTTAACAACGTACCACCGGAGCTGAACGATATGTGCCGCATCATCAGCCATGccc                                                                                                                                                  >  1:437527/1‑150 (MQ=255)

 cagcagCAACCCAGGCTTCCTTGTGGAAGATTGCCGGGTACGAGTACGTCTGAATAACCAAAGCTGGGTGTTAAACATCGACAGCAAAGGTCAGTTTAACAACGTACCACCGGAGCTGAACGATATGTGCCGCATCATCAGCCATGCccac                                                                                                                                                >  1:60773/1‑151 (MQ=255)

     agcaACCCAGGCTTCCTTGTGGAAGATTGCCGGGTACGAGTACGTCTGAATAACCAAAGCTGGGTGTTAAACATCGACAGCAAAGGTCAGTTTAACAACGTACCACCGGAGCTGAACGATATGTGCCGCATCATCAGCCATGCCCACCAGc                                                                                                                                            <  2:745897/151‑1 (MQ=255)

      gcaACCCAGGCTTCCTTGTGGAAGATTGCCGGGTACGAGTACGTCTGAATAACCAAAGCTGGGTGTTAAACATCGACAGCAAAGGTCAGTTTAACAACGTACCACCGGAGCTGAACGATATGTGCCGCATCATCAGCCATGCCCACCAGc                                                                                                                                            >  2:595369/1‑150 (MQ=255)

       caACCCAGGCTTCCTTGTGGAAGATTGCCGGGTACGAGTACGTCTGAATAACCAAAGCTGGGTGTTAAACATCGACAGCAAAGGTCAGTTTAACAACGTACCACCGGAGCTGAACGATATGTGCCGCATCATCAGCCATGCCCACCAGcat                                                                                                                                          >  2:714093/1‑151 (MQ=255)

           ccAGGCTTCCTTGTGGAAGATTGCCGGGTACGAGTACGTCTGAATAACCAAAGCTGGGTGTTAAACATCGACAGCAAAGGTCAGTTTAACAACGTACCACCGGAGCTGAACGATATGTGCCGCATCATCAGCCATGCCCACCAGcatcatc                                                                                                                                      <  2:698333/151‑1 (MQ=255)

             aGGCTTCCTTGTGGAAGATTGCCGGGTACGAGTACGTCTGAATAACCAAAGCTGGGTGTTAAACATCGACAGCAAAGGTCAGTTTAACAACGTACCACCGGAGCTGAACGATATGTGCCGCATCATCAGCCATGCCCACCAGcatcatcat                                                                                                                                    <  2:51459/151‑1 (MQ=255)

              ggCTTCCTTGTGGAAGATTGCCGGGTACGAGTACGTCTGAATAACCAAAGCTGGGTGTTAAACATCGACAGCAAAGGTCAGTTTAACAACGTACCACCGGAGCTGAACGATATGTGCCGCATCATCAGCCATGCCCACCAGCATCATCAtt                                                                                                                                   >  1:24279/1‑151 (MQ=255)

               gCTTCCTTGTGGAAGATTGCCGGGTACGAGTACGTCTGAATAACCAAAGCTGGGTGTTAAACATCGACAGCAAAGGTCAGTTTAACAACGTACCACCGGAGCTGAACGATATGTGCCGCATCATCAGCCATGCCCACCAGCATCATCAtt                                                                                                                                   >  1:172550/1‑150 (MQ=255)

                  tCCTTGTGGAAGATTGCCGGGTACGAGTACGTCTGAATAACCAAAGCTGGGTGTTAAACATCGACAGCAAAGGTCAGTTTAACAACGTACCACCGGAGCTGAACGATATGTGCCGCATCATCAGCCATGCCCACCAGCATCATCATTATc                                                                                                                                <  2:589238/150‑1 (MQ=255)

                   ccTTGTGGAAGATTGCCGGGTACGAGTACGTCTGAATAACCAAAGCTGGGTGTTAAACATCGACAGCAAAGGTCAGTTTAACAACGTACCACCGGAGCTGAACGATATGTGCCGCATCATCAGCCATGCCCACCAGCATCATCATTATctg                                                                                                                              <  1:307027/151‑1 (MQ=255)

                              aTTGCCGGGTACGAGTACGTCTGAATAACCAAAGCTGGGTGTTAAACATCGACAGCAAAGGTCAGTTTAACAACGTACCACCGGAGCTGAACGATATGTGCCGCATCATCAGCCATGCCCACCAGCATCATCATTATCTGCTGGGCCGCGt                                                                                                                   >  2:594938/1‑151 (MQ=255)

                                 gCCGGGTACGAGTACGTCTGAATAACCAAAGCTGGGTGTTAAACATCGACAGCAAAGGTCAGTTTAACAACGTACCACCGGAGCTGAACGATATGTGCCGCATCATCAGCCATGCCCACCAGCATCATCATTATCTGCTGGGCCGCGTgg                                                                                                                 >  1:141405/1‑150 (MQ=255)

                                   cGGGTACGAGTACGTCTGAATAACCAAAGCTGGGTGTTAAACATCGACAGCAAAGGTCAGTTTAACAACGTACCACCGGAGCTGAACGATATGTGCCGCATCATCAGCCATGCCCACCAGCATCATCATTATCTGCTGGGCCGCGTGGAgg                                                                                                              <  1:843246/151‑1 (MQ=255)

                                     ggTACGAGTACGTCTGAATAACCAAAGCTGGGTGTTAAACATCGACAGCAAAGGTCAGTTTAACAACGTACCACCGGAGCTGAACGATATGTGCCGCATCATCAGCCATGCCCACCAGCATCATCATTATCTGCTGGGCCGCGTGGAGgtg                                                                                                            <  1:631144/151‑1 (MQ=255)

                                     ggTACGAGTACGTCTGAATAACCAAAGCTGGGTGTTAAACATCGACAGCAAAGGTCAGTTTAACAACGTACCACCGGAGCTGAACGATATGTGCCGCATCATCAGCCATGCCCACCAGCATCATCATTATCTGCTGGGCCGCGTGGAGgt                                                                                                             <  2:54671/150‑1 (MQ=255)

                                           aGTACGTCTGAATAACCAAAGCTGGGTGTTAAACATCGACAGCAAAGGTCAGTTTAACAACGTACCACCGGAGCTGAACGATATGTGCCGCATCATCAGCCATGCCCACCAGCATCATCATTATCTGCTGGGCCGCGTGGAGGTGTAATc                                                                                                       >  2:49803/1‑150 (MQ=255)

                                            gTACGTCTGAATAACCAAAGCTGGGTGTTAAACATCGACAGCAAAGGTCAGTTTAACAACGTACCACCGGAGCTGAACGATATGTGCCGCATCATCAGCCATGCCCACCAGCATCATCATTATCTGCTGGGCCGCGTGGAGGTGTAATCAt                                                                                                     <  1:557260/151‑1 (MQ=255)

                                                gTCTGAATAACCAAAGCTGGGTGTTAAACATCGACAGCAAAGGTCAGTTTAACAACGTACCACCGGAGCTGAACGATATGTGCCGCATCATCAGCCATGCCCACCAGCATCATCATTATCTGCTGGGCCGCGTGGAGGTGTAATCATGTTg                                                                                                 >  2:637325/1‑151 (MQ=255)

                                                    gAATAACCAAAGCTGGGTGTTAAACATCGACAGCAAAGGTCAGTTTAACAACGTACCACCGGAGCTGAACGATATGTGCCGCATCATCAGCCATGCCCACCAGCATCATCATTATCTGCTGGGCCGCGTGGAGGTGTAATCATGTTGATTc                                                                                             >  2:707318/1‑151 (MQ=255)

                                                     gctcttccgatcTGGGTGTTAAACATCGACAGCAAAGGTCAGTTTAACAACGTACCACCGGAGCTGAACGATATGTGCCGCATCATCAGCCATGCCCACCAGCATCATCATTATCTGCTGGGCCGCGTGGAGGTGTAATCATGTTGATTCt                                                                                            >  2:104904/12‑151 (MQ=255)

                                                        aaCCAAAGCTGGGTGTTAAACATCGACAGCAAAGGTCAGTTTAACAACGTACCACCGGAGCTGAACGATATGTGCCGCATCATCAGCCATGCCCACCAGCATCATCATTATCTGCTGGGCCGCGTGGAGGTGTAATCATGTTGATTCTgaa                                                                                         <  2:490313/151‑1 (MQ=255)

                                                         aCCAAAGCTGGGTGTTAAACATCGACAGCAAAGGTCAGTTTAACCACGTACCACCGGAGCTGAACGATATGTGCCGCATCATCAGCCATGCCCACCAGCATCATCATTATCTGCTGGGCCGCGTGGAGCTGTAATCATGTTGCTTCTg                                                                                           >  1:3198/1‑148 (MQ=255)

                                                             aaGCTGGGTGTTAAACATCGACAGCAAAGGTCAGTTTAACAACGTACCACCGGAGCTGAACGATATGTGCCGCATCATCAGCCATGCCCACCAGCATCATCATTATCTGCTGGGCCGCGTGGAGGTGTAATCATGTTGATTCTGAAGAATg                                                                                    >  1:168089/1‑151 (MQ=255)

                                                                  gggTGTTAAACATCGACAGCAAAGGTCAGTTTAACAACGTACCACCGGAGCTGAACGATATGTGCCGCATCATCAGCCATGCCCACCAGCATCATCATTATCTGCTGGGCCGCGTGGAGGTGTAATCATGTTGATTCTGAAGAATGTCa                                                                                 <  2:193138/149‑1 (MQ=255)

                                                                   ggTGTTAAACATCGACAGCAAAGGTCAGTTTAACAACGTACCACCGGAGCTGAACGATATGTGCCGCATCATCAGCCATGCCCACCAGCATCATCATTATCTGCTGGGCCGCGTGGAGGTGTAATCATGTTGATTCTGAAGAATGTCACTg                                                                              >  1:705082/1‑151 (MQ=255)

                                                                         aaaCATCGACAGCAAAGGTCAGTTTAACAACGTACCACCGGAGCTGAACGATATGTGCCGCATCATCAGCCATGCCCACCAGCATCATCATTATCTGCTGGGCCGCGTGGAGGTGTAATCATGTTGATTCTGAAGAATGTCACTGCGGTAc                                                                        >  2:43605/1‑151 (MQ=255)

                                                                                gACAGCAAAGGTCAGTTTAACAACGTACCACCGGAGCTGAACGATATGTGCCGCATCATCAGCCATGCCCACCAGCATCATCATTATCTGCTGGGCCGCGTGGAGGTGTAATCATGTTGATTCTGAAGAATGTCACTGCGGTACAGCTac                                                                  >  1:703991/1‑150 (MQ=255)

                                                                                 aCAGCAAAGGTCAGTTTAACAACGTACCACCGGAGCTGAACGATATGTGCCGCATCATCAGCCATGCCCACCAGCATCATCATTATCTGCTGGGCCGCGTGGAGGTGTAATCATGTTGATTCTGAAGAATGTCACTGCGGTACAGCTacac                                                                <  2:835409/151‑1 (MQ=255)

                                                                                   aGCAAAGGTCAGTTTAACAACGTACCACCGGAGCTGAACGATATGTGCCGCATCATCAGCCATGCCCACCAGCATCATCATTATCTGCTGGGCCGCGTGGAGGTGTAATCATGTTGATTCTGAAGAATGTCACTGCGGTACAGCTACAccc                                                              <  1:484403/151‑1 (MQ=255)

                                                                                       aaGGTCAGTTTAACAACGTACCACCGGAGCTGAACGATATGTGCCGCATCATCAGCCATGCCCACCAGCATCATCATTATCTGCTGGGCCGCGTGGAGGTGTAATCATGTTGATTCTGAAGAATGTCACTGCGGTACAGCTACACCCGGc                                                           >  1:581714/1‑150 (MQ=255)

                                                                                        aGGTCAGTTTAACAACGTACCACCGGAGCTGAACGATATGTGCCGCATCATCAGCCATGCCCACCAGCATCATCATTATCTGCTGGGCCGCGTGGAGGTGTAATCATGTTGATTCTGAAGAATGTCACTGCGGTACAGCTACACCCGGCa                                                          >  1:452006/1‑150 (MQ=255)

                                                                                                   acaacGTACCACCGGAGCTGAACGATATGTGCCGCATCATCAGCCATGCCCACCAGCATCATCATTATCTGCTGGGCCGCGTGGAGGTGTAATCATGTTGATTCTGAAGAATGTCACTGCGGTACAGCTACACCCGGCAAAAGTGCAGGaa                                              <  2:373013/151‑1 (MQ=255)

                                                                                                      acGTACCACCGGAGCTGAACGATATGTGCCGCATCATCAGCCATGCCCACCAGCATCATCATTATCTGCTGGGCCGCGTGGAGGTGTAATCATGTTGATTCTGAAGAATGTCACTGCGGTACAGCTACACCCGGCAAAAGTGCAGGAAGGc                                           >  2:521943/1‑151 (MQ=255)

                                                                                                             accGGAGCTGAACGATATGTGCCGCATCATCAGCCATGCCCACCAGCATCATCATTATCTGCTGGGCCGCGTGGAGGTGTAATCATGTTGATTCTGAAGAATGTCACTGCGGTACAGCTACACCCGGCAAAAGTGCAGGAAGGCGTTGat                                     >  1:352421/1‑150 (MQ=255)

                                                                                                                ggAGCTGAACGATATGTGCCGCATCATCAGCCATGCCCACCAGCATCATCATTATCTGCTGGGCCGCGTGGAGGTGTAATCATGTTGATTCTGAAGAATGTCACTGCGGTACAGCTACACCCGGCAAAAGTGCAGGAAGGCGTTGATATCg                                 <  1:691246/151‑1 (MQ=255)

                                                                                                                     tGAACGATATGTGCCGCATCATCAGCCATGCCCACCAGCATCATCATTATCTGCTGGGCCGCGTGGAGGTGTAATCATGTTGATTCTGAAGAATGTCACTGCGGTACAGCTACACCCGGCAAAAGCGCAGGAAGGCGTTGATATCGCCAt                             >  1:429016/1‑150 (MQ=255)

                                                                                                                      gAACGATATGTGCCGCATCATCAGCCATGCCCACCAGCATCATCATTATCTGCTGGGCCGCGTGGAGGTGTAATCATGTTGATTCTGAAGAATGTCACTGCGGTACAGCTACACCCGGCAAAAGTGCAGGAAGGCGTTGATATCGCCATc                            <  2:805948/150‑1 (MQ=255)

                                                                                                                           atatGTGCCGCATCATCAGCCATGCCCACCAGCATCATCATTATCTGCTGGGCCGCGTGGAGGTGTAATCATGTTGATTCTGAAGAATGTCACTGCGGTACAGCTACACCCGGCAAAAGTGCAGGAAGGCGTTGATATCGCCATCGAAAAc                      <  1:382755/151‑1 (MQ=255)

                                                                                                                                    gCATCATCAGCCATGCCCACCAGCATCATCATTATCTGCTGGGCCGCGTGGAGGTGTAATCATGTTGATTCTGAAGAATGTCACTGCGGTACAGCTACACCCGGCAAAAGTGCAGGAAGGCGTTGATATCGCCATCGAAAACGATGTGAtt             >  1:500742/1‑151 (MQ=255)

                                                                                                                                     catcatCAGCCATGCCCACCAGCATCATCATTATCTGCTGGGCCGCGTGGAGGTGTAATCATGTTGATTCTGAAGAATGTCACTGCGGTACAGCTACACCCGGCAAAAGTGCAGGAAGGCGTTGATATCGCCATCGAAAACGATGTGATTg            >  2:211082/1‑151 (MQ=255)

                                                                                                                                       tcatcaGCCATGCCCACCAGCATCATCATTATCTGCTGGGCCGCGTGGAGGTGTAATCATGTTGATTCTGAAGAATGTCACTGCGGTACAGCTACACCCGGCAAAAGTGCAGGAAGGCGTTGATATCGCCATCGAAAACGATGTGATTGTc          >  1:853048/1‑151 (MQ=255)

                                                                                                                                               cATGCCCACCAGCATCATCATTATCTGCTGGGCCGCGTGGAGGTGTAATCATGTTGATTCTGAAGAATGTCACTGCGGTACAGCTACACCCGGCAAAAGTGCAGGAAGGCGTTGATATCGCCATCGAAAACGATGTGATTGTCGCTATCgg  <  1:754743/151‑1 (MQ=255)

ACAGCAGCAACCCAGGCTTCCTTGTGGAAGATTGCCGGGTACGAGTACGTCTGAATAACCAAAGCTGGGTGTTAAACATCGACAGCAAAGGTCAGTTTAACAACGTACCACCGGAGCTGAACGATATGTGCCGCATCATCAGCCATGTCCACCAGCATCATCATTATCTGCTGGGCCGCGTGGAGGTGTAATCATGTTGATTCTGAAGAATGTCACTGCGGTACAGCTACACCCGGCAAAAGTGCAGGAAGGCGTTGATATCGCCATCGAAAACGATGTGATTGTCGCTATCGG  >  NC_000913/3018968‑3019261

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A