Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A3 F50 I1 R1	300	54.7	1718999	98.4%	1691495	149.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,039,247	A→G	V166A (GTG→GCG)	xerD ←	site‑specific tyrosine recombinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,039,247	0	A	G	100.0%	164.6 / NA	47	V166A (GTG→GCG)	xerD	site‑specific tyrosine recombinase
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (22/25); total (22/25)

CGCACGCTGGCTGGGAAACAACACGTCAATTGACACACCATTCAGCAGCCACGGACGCCCATGTTCCAGATAGGTTTCCAGCCAGTAAACCGCCTCTTCACCTAACGGCACCAGACGCTCTTTGTTGCCTTTACCAATGACCCGTACCACGCCCTGACGCAGGCTGATATCACTCATTGTCAGTCCGACCAGTTCAGAGACACGCAGCCCGGTAGCATACAACACTTCAAGCATGGCTTTATCGCGTAGCTCCAGTGGCTGATCGATTAATGGTGCCTGTAATAAACGTTCGAC  >  NC_000913/3039099‑3039392

cgcACGCTGGCTGGGAAACAACACGTCAATTGACACACCATTCAGCAGCCCCGGACGCCCATGTTCCAGATAGGTTTCCAGCCAGTAAACCGCCTCTTCACCTAACGGCACCAGACGCTCTTTGTTGCCTTTACCAATGACCCGTACcgcg                                                                                                                                                 <  2:316320/151‑1 (MQ=255)

cgcACGCTGGCTGGGAAACAACACGTCAATTGACACACCATTCAGCAGCCACGGACGCCCATGTTCCAGATAGGTTTCCAGCCAGTAAACCGCCTCTTCACCTAACGGCACCAGACGCTCTTTGTTGCCTTTACCAATGACCCGTACcgcg                                                                                                                                                 >  2:600547/1‑151 (MQ=255)

           tggGAAACAACACGTCAATTGACACACCATTTAGCAGCCACGGACGCCCATGTTCCAGATAGGTTTCCAGCCAGTAAACCGCCTCTTCACCTAACGGCACCAGACGCTCTTTGTTGCCTTTACCAATGACCCGTACCGCGCCCTGACGc                                                                                                                                        >  1:325856/1‑149 (MQ=255)

            gggAAACAACACGTCAATTGACACACCATTCAGCAGCCACGGACGCCCATGTTCCAGATAGGTTTCCAGCCAGTAAACCGCCTCTTCACCTAACGGCACCAGACGCTCTTTGTTGCCTTTACCAATGACCCGTACCGCGCCCTGACGCAg                                                                                                                                      <  2:15424/150‑1 (MQ=255)

                 acaacaCGTCAATTGACACACCATTCAGCAGCCACGGACGCCCATGTTCCAGATAGGTTTCCAGCCAGTAAACCGCCTCTTCACCTAACGGCACCAGACGCTCTTTGTTGCCTTTACCAATGACCCGTACCGCGCCCTGACGCAGGCTGat                                                                                                                                >  2:539875/1‑151 (MQ=255)

                     cacGTCAATTGCCACACCATTCAGCACCCACGGACGCCCATGTTCCAGATAGGTTTCCAGCCAGTAAACCGCCTCTTCACCTAACGGCACCAGACGCTCTTTGTTGCCTTTACCAATGACCCGTACCGCGCCCTGACGCAGGCTGATATc                                                                                                                             <  1:729283/150‑1 (MQ=255)

                       cGTCAATTGACACACCATTCAGCAGCCACGGACGCCCATGTTCCAGATAGGTTTCCAGCCAGTAAACCGCCTCTTCACCTAACGGCACCAGACGCTCTTTGTTGCCTTTACCAATGACCCGTACCGCGCCCTGACGCAGGCTGATATCACt                                                                                                                          >  1:297147/1‑151 (MQ=255)

                           aaTTGACACCCCATCCAGCAGCCCCGGACGCCCATGTTCCAGATAGGTTTCCAGCCAGTAAACCGCCTCTTCACCTAACGGCACCAGACGCTCTTTGTTGCCTTTACCAATGACCCGTACCGCGCCCTGACGCAGGCTGATATCACTCAtt                                                                                                                      <  1:711811/151‑1 (MQ=255)

                           aaTTGACACACCATTCAGCAGCCACGGACGCCCATGTTCCAGATAGGTTTCCAGCCAGTAAACCGCCTCTTCACCTAACGGCACCAGACGCTCTTTGTTGCCTTTACCAATGACCCGTACCGCGCCCTGACGCAGGCTGATATCAc                                                                                                                           <  1:116416/146‑1 (MQ=255)

                             ttGACACACCATTCAGCAGCCACGGCCGCCCATGTTCCAAATAGGTTTCCAGCCAGTAAACCGCCTCTTCACCTAACGGCACCAGACGCTCTTTGTTGCCTTTACCAATGACCCGTACCGCGCCCTGACGCAGGCTGATATCACTCATTg                                                                                                                     <  2:641891/150‑1 (MQ=255)

                               gACACACCATTCAGCAGCCACGGACGCCCATGTTCCAGATAGGTTTCCAGCCAGTAAACCGCCTCTTCACCTAACGGCACCAGACGCTCTTTGTTGCCTTTACCAATGACCCGTACCGCGCCCTGACGCAGGCTGATATCACTCATTGTc                                                                                                                   >  1:835987/1‑150 (MQ=255)

                                           agcagcCACGGACGCCCATGTTCCAGATAGGTTTCCAGCCAGTAAACCGCCTCTTCACCTAACGGCACCAGACGCTCTTTGTTGCCTTTACCAATGACCCGTACCGCGCCCTGACGCAGGCTGATATCACTCATTGTCAGTCCGACCAGtt                                                                                                      >  1:200573/1‑151 (MQ=255)

                                             cagcCACGGACGCCCATGTTCCAGATAGGTTTCCAGCCAGTAAACCGCCTCTTCACCTAACGGCACCAGACGCTCTTTGTTGCCTTTACCAATGACCCGTACCGCGCCCTGACGCAGGCTGATATCACTCATTGTCAGTCCGACCAGTTc                                                                                                     >  1:199871/1‑150 (MQ=255)

                                                ccccGGACGCCCATGTTCCAGATGGGTTCCCAGCCAGTAAACCGCCTCTTCACCTAACGGCACCAGACGCTCTTTGTTGCCTTTACCAATGACCCGTACCGCGCCCTGACGCAGGCTGATATCACTCATTGTCAGTCCGACCAGTTCa                                                                                                    <  1:64797/145‑1 (MQ=255)

                                                 cACGGACGCCCATGTTCCAGATAGGTTTCCAGCCAGTAAACCGCCTCTTCACCTAACGGCACCAGACGCTCTTTGTTGCCTTTACCAATGACCCGTACCGCGCCCTGACGCAGGCTGATATCACTCATTGTCAGTCCGACCAGTTCAgaga                                                                                                <  2:267831/151‑1 (MQ=255)

                                                      aCGCCCATGTTCCAGATAGGTTTCCAGCCAGTAAACCGCCTCTTCACCTAACGGCACCAGACGCTCTTTGTTGCCTTTACCAATGACCCGTACCGCGCCCTGACGCAGGCTGATATCACTCATTGTCAGTCCGACCAGTTCAGAGACACGc                                                                                           >  1:740640/1‑151 (MQ=255)

                                                      aCGCCCATGTTCCAGATAGGTTTCCAGCCAGTAAACCGCCTCTTCACCTAACGGCACCAGACGCTCTTTGTTGCCTTTACCAATGACCCGTACCGCGCCCTGACGCAGGCTGATATCACTCATTGTCAGTCCGACCAGTTCAGAGACACGc                                                                                           >  1:377906/1‑151 (MQ=255)

                                                        gCCCATGTTCCAGATAGGTTTCCAGCCAGTAAACCGCCTCTTCACCTAACGGCACCAGACGCTCTTTGTTGCCTTTACCAATGACCCGTACCGCGCCCTGACGCAGGCTGATATCACTCATTGTCAGTCCGACCAGTTCAGAGACACGc                                                                                           >  1:708611/1‑149 (MQ=255)

                                                         cccATGTTCCAGATAGGTTTCCAGCCAGTAAACCGCCTCTTCACCTAACGGCACCAGACGCTCTTTGTTGCCTTTACCAATGACCCGTACCGCGCCCTGACGCAGGCTGATATCACTCATTGTCAGTCCGACCAGTTCAGAGACACGCAGc                                                                                        <  2:207296/151‑1 (MQ=255)

                                                         cccATGTTCCAGAAAGGTTCCCAGCCAGTAAACCGCCTCTTCACCTAACGGCACCAGACGCTCTTTGTTGCCTTTACCAATGACCCGTACCGCGCCCTGACGCAGGCTGATATCACTCATTGTCAGTCCGACCAGTTCAGAGACACGCAg                                                                                         <  1:850734/150‑1 (MQ=255)

                                                          ccATGTTCCAGATAGGTTTCCAGCCAGTAAACCGCCTCTTCACCTAACGGCACCAGACGCTCTTTGTTGCCTTTACCAATGACCCGTACCGCGCCCTGACGCAGGCTGATATCACTCATTGTCAGTCCGACCAGTTCAGAGACACGCAGcc                                                                                       >  2:683459/1‑151 (MQ=255)

                                                           cATGTTCCAGATAGGTTTCCAGCCAGTAAACCGCCTCTTCACCTAACGGCACCAGACGCTCTTTGTTGCCTTTACCAATGACCCGTACCGCGCCCTGACGCAGGCTGATATCACTCATTGTCAGTCCGACCAGTTCAGAGACACGCAGccc                                                                                      >  2:529703/1‑151 (MQ=255)

                                                                  cAGATAGGTTTCCAGCCAGTAAACCGCCTCTTCACCTAACGGCACCAGACGCTCTTTGTTGCCTTTACCAATGACCCGTACCGCGCCCTGACGCAGGCTGATATCACTCATTGTCAGTCCGACCAGTTCAGAGACACGCAGCCCGGTAGc                                                                                >  1:444866/1‑150 (MQ=255)

                                                                    gATAGGTTTCCAGCCAGTAAACCGCCTCTTCACCTAACGGCACCAGACGCTCTTTGTTGGCTTTACCAATGACCCGTACCGCGCCCTGACGCAGGCTGATATCACTCATTGTCAGTCCGACCAGTTCAGAGACACGCAGCCCGGTAGCAt                                                                              >  1:373628/1‑150 (MQ=255)

                                                                        ggTTTCCAGCCAGTAAACCGCCTCTTCACCTAACGGCACCAGACGCTCTTTGTTGCCTTTACCAATGACCCGTACCGCGCCCTGACGCAGGCTGATATCACTCATTGTCAGTCCGACCAGTTCAGAGACACGCAGCCCGGTAGCATacaa                                                                          <  2:574016/150‑1 (MQ=255)

                                                                                 ccagTAAACCGCCTCTTCACCTAACGGCACCAGACGCTCTTTGTTGCCTTTACCAATGACCCGTACCGCGCCCTGACGCAGGCTGATATCACTCATTGTCAGTCCGACCAGTTCAGAGACACGCAGCCCGGTAGCATACAACACTTCAAGc                                                                >  1:313303/1‑151 (MQ=255)

                                                                                  cagTAAACCGCCTCTTCACCTAACGGCACCAGACGCTCTTTGTTGCCTTTACCAATGACCCGTACCGCGCCCTGACGCAGGCTGATATCACTCATTGTCAGTCCGACCAGTTCAGAGACACGCAGCCCGGTAGCATACAACACTTCAAGc                                                                >  1:110585/1‑150 (MQ=255)

                                                                                               ctTCACCTAACGGCACCAGACGCTCTTTGTTGCCTTTACCAATGACCCGTACCGCGCCCTGACGCAGGCTGATATCACTCATTGTCAGTCCGACCAGTTCAGAGACACGCAGCCCGGTAGCATACAACACTTCAAGCATGGCTTTATcg                                                    <  2:533647/149‑1 (MQ=255)

                                                                                                  cACCTAACGGCACCAGACGCTCTTTGTTGCCTTTACCAATGACCCGTACCGCGCCCTGACGCAGGCTGATATCACTCATTGTCAGTCCGACCAGTTCAGAGACACGCAGCCCGGTAGCATACAACACTTCAAGCATGGCTTTATCGCGTa                                                <  2:720739/150‑1 (MQ=255)

                                                                                                  cACCTAACGGCACCAGACGCTCTTTGTTGCCTTTACCAATGACCCGTACCGCGCCCTGACGCAGGCTGATATCACTCATTGTCAGTCCGACCAGTTCAGAGACACGCAGCCCGGTAGCATACAACACTTCAAGCATGGCTTTATCGCGTa                                                <  2:186591/150‑1 (MQ=255)

                                                                                                       aaCGGCACCAGACGCTCTTTGTTGCCTTTACCAATGACCCGTACCGCGCCCTGACGCAGGCTGATATCACTCATTGTCAGTCCGACCAGTTCAGAGACACGCAGCCCGGTAGCATACAACACTTCAAGCATGGCTTTATCGCGTAGCTCCa                                          <  2:848120/151‑1 (MQ=255)

                                                                                                         cGGCACCAGACGCTCTTTGTTGCCTTTACCAATGACCCGTACCGCGCCCTGACGCAGGCTGATATCACTCATTGTCAGTCCGACCAGTTCAGAGACACGCAGCCCGGTAGCATACAACACTTCAAGCATGGCTTTATCGCGTAGCTCCAGt                                        <  2:377930/151‑1 (MQ=255)

                                                                                                         cGGCACCAGACGCTCTTTGTTGCCTTTACCAATGACCCGTACCGCGCCCTGACGCAGGCTGATATCACTCATTGTCAGTCCGACCAGTTCAGAGACACGCAGCCCGGTAGCATACAACACTTCAAGCATGGCTTTATCGCGTAGCTCCAGt                                        <  2:740683/151‑1 (MQ=255)

                                                                                                          ggCACCAGACGCTCTTTGTTGCCTTTACCAATGACCCGTACCGCGCCCTGACGCAGGCTGATATCACTCATTGTCAGTCCGACCAGTTCAGAGACACGCAGCCCGGTAGCATACAACACTTCAAGCATGGCTTTATCGCGTAGCTCCAGTg                                       <  2:326752/151‑1 (MQ=255)

                                                                                                                aGACGCTCTTTGTTGCCTTTACCAATGACCCGTACCGCGCCCTGACGCAGGCTGATATCACTCATTGTCAGTCCGACCAGTTCAGAGACACGCAGCCCGGTAGCATACAACACTTCAAGCATGGCTTTATCGCGTAGCTCCAGTGGCTGa                                  >  1:761274/1‑150 (MQ=255)

                                                                                                                aGACGCTCTTTGTTGCCTTTACCAATGACCCGTACCGCGCCCTGACGCAGGCTGATATCACTCATTGTCAGTCCGACCAGTTCAGAGACACGCAGCCCGGTAGCATACAACACTTCAAGCATGGCTTTATCGCGTAGCTCCAGTGGCTGAt                                 >  2:622150/1‑151 (MQ=255)

                                                                                                                  aCGCTCTTTGTTGCCTTTACCAATGACCCGTACCGCGCCCTGACGCAGGCTGATATCACTCATTGTCAGTCCGACCAGTTCAGAGACACGCAGCCCGGTAGCATACAACACTTCAAGCATGGCTTTATCGCGTAGCTCCAGTGGCTGATCg                               <  1:539838/151‑1 (MQ=255)

                                                                                                                   cGCTCTTTGTTGCCTTTACCAATGACCCGTACCGCGCCCTGACGCAGGCTGATATCACTCATTGTCAGTCCGACCAGTTCAGAGACACGCAGCCCGGTAGCATACAACACTTCAAGCATGGCTTTATCGCGTAGCTCCAGTGGCTGATCg                               >  1:685856/1‑150 (MQ=255)

                                                                                                                        tttGTTGCCTTTACCAATGACCCGTACCGCGCCCTGACGCAGGCTGATATCACTCATTGTCAGTCCGACCAGTTCAGAGACACGCAGCCCGGTAGCATACAACACTTCAAGCATGGCTTTATCGCGTAGCTCCAGTGGCTGATCGATTAAt                         >  2:827574/1‑151 (MQ=255)

                                                                                                                              gCCTTTACCAATGACCCGTACCGCGCCCTGACGCAGGCTGATATCACTCATTGTCAGTCCGACCAGTTCAGAGACACGCAGCCCGGTAGCATACAACACTTCAAGCATGGCTTTATCGCGTAGCTCCAGTGGCTGATCGATTAATGGTGcc                   >  1:696824/1‑151 (MQ=255)

                                                                                                                               ccTTTACCAATGACCCGTACCGCGCCCTGACGCAGGCTGATATCACTCATTGTCAGTCCGACCAGTTCAGAGACACGCAGCCCGGTAGCATACAACACTTCAAGCATGGCTTTATCGCGTAGCTCCAGTGGCTGATCGATTAATGGTGCCt                  <  1:318150/151‑1 (MQ=255)

                                                                                                                                   tACCAATGACCCGTACCGCGCCCTGACGCAGGCTGATATCACTCATTGTCAGTCCGACCAGTTCAGAGACACGCAGCCCGGTAGCATACAACACTTCAAGCATGGCTTTATCGCGTAGCTCCAGTGGCTGATCGATTAATGGTGCCTGta               <  1:767255/150‑1 (MQ=255)

                                                                                                                                     ccAATGACCCGTACCGCGCCCTGACGCAGGCTGATATCACTCATTGTCAGTCCGACCAGTTCAGAGACACGCAGCCCGGTAGCATACAACACTTCAAGCATGGCTTTATCGCGTAGCTCCAGTGGCTGATCGATTAATGGTGCCTGtaata            <  1:363384/151‑1 (MQ=255)

                                                                                                                                        aTGACCCGTACCGCGCCCTGACGCAGGCTGATATCACTCATTGTCAGTCCGACCAGTTCAGAGACACGCAGCCCGGTAGCATACAACACTTCAAGCATGGCTTTATCGCGTAGCTCCAGTGGCTGATCGATTAATGGTGCCTGTAATAAAc         <  1:286091/151‑1 (MQ=255)

                                                                                                                                        aTGACCCGTACCGCGCCCTGACGCAGGCTGATATCACTCATTGTCAGTCCGACCAGTTCAGAGACACGCAGCCCGGTAGCATACAACACTTCAAGCATGGCTTTATCGCGTAGCTCCAGTGGCTGATCGATTAATGGTGCCTGTAATAAAc         <  1:217134/151‑1 (MQ=255)

                                                                                                                                          gACCCGTACCGCGCCCTGACGCAGGCTGATATCACTCATTGTCAGTCCGACCAGTTCAGAGACACGCAGCCCGGTAGCATACAACACTTCAAGCATGGCTTTATCGCGTAGCTCCAGTGGCTGATCGATTAATGGTGCCTGTAATAAACGt       >  2:240312/1‑151 (MQ=255)

                                                                                                                                           aCCCGTACCGCGCCCTGACGCAGGCTGATATCACTCATTGTCAGTCCGACCAGTTCAGAGACACGCAGCCCGGTAGCATACAACACTTCAAGCATGGCTTTATCGCGTAGCTCCAGTGGCTGATCGATTAATGGTGCCTGTAATAAACGtt      <  1:160768/151‑1 (MQ=255)

                                                                                                                                             ccGTACCGCGCCCTGACGCAGGCTGATATCACTCATTGTCAGTCCGACCAGTTCAGAGACACGCAGCCCGGTAGCATACAACACTTCAAGCATGGCTTTATCGCGTAGCTCCAGTGGCTGATCGATTAATGGTGCCTGTAATAAACGTTCg    >  1:129452/1‑151 (MQ=255)

                                                                                                                                                tACCGCGCCCTGACGCAGGCTGATATCACTCATTGTCAGTCCGACCAGTTCAGAGACACGCAGCCCGGTAGCATACAACACTTCAAGCATGGCTTTATCGCGTAGCTCCAGTGGCTGATCGATTAATGGTGCCTGTAATAAACGTTCGAc  <  2:575823/150‑1 (MQ=255)

CGCACGCTGGCTGGGAAACAACACGTCAATTGACACACCATTCAGCAGCCACGGACGCCCATGTTCCAGATAGGTTTCCAGCCAGTAAACCGCCTCTTCACCTAACGGCACCAGACGCTCTTTGTTGCCTTTACCAATGACCCGTACCACGCCCTGACGCAGGCTGATATCACTCATTGTCAGTCCGACCAGTTCAGAGACACGCAGCCCGGTAGCATACAACACTTCAAGCATGGCTTTATCGCGTAGCTCCAGTGGCTGATCGATTAATGGTGCCTGTAATAAACGTTCGAC  >  NC_000913/3039099‑3039392

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A