Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A3 F50 I1 R1	300	54.7	1718999	98.4%	1691495	149.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,296,292	C→T	R162C (CGC→TGC)	diaA →	DnaA initiator‑associating factor for replication initiation

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,296,292	0	C	T	100.0%	194.7 / NA	52	R162C (CGC→TGC)	diaA	DnaA initiator‑associating factor for replication initiation
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (22/30); total (22/30)

TTGTTAGCCATTTCCACCCGTGGCAACAGCCGCGATATTGTTAAAGCAGTTGAAGCCGCCGTTACGCGTGATATGACCATTGTGGCATTGACCGGCTATGACGGCGGCGAACTTGCAGGTTTGTTAGGGCCACAGGATGTGGAGATCCGCATTCCTTCGCATCGTAGTGCTCGCATTCAGGAAATGCATATGCTGACGGTAAATTGCCTGTGCGATCTGATCGATAACACGCTTTTCCCTCACCAGGATGATTAAGGAGAATACATGAAGGCATTATCGCCAATCGCAGTCCT  >  NC_000913/3296145‑3296437

ttGTTAGCCATTTCCACCCGTGGCAACAGCCGCGATATTGTTAAAGCAGTTGAAGCCGCCGTTACGCGTGATATGACCATTGTGGCATTGACCGGCTATGACGGCGGCGAACTTGCAGGTTTGTTAGGGCCACAGGATGTGGAGATCTGc                                                                                                                                                 >  1:814750/1‑150 (MQ=255)

  gTTAGCCATTTCCACCCGTGGCAACAGCCGCGATATTGTTAAAGCAGTTGAAGCCGCCGTTACGCGTGATATGACCATTGTGGCATTGACCGGCTATGACGGCGGCGAACTTGCAGGTTTGTTAGGGCCACAGGATGTGGAGATCTGCAt                                                                                                                                               <  1:601344/150‑1 (MQ=255)

  gTTAGCCATTCCCCCCCGTGGCACCAGCCGGGATATTGTTAAAGCAGTTGAAGCCGCCGTTACGCGTGATATGACCATTGTGGCATTGACCGGCTATGACGGCGGCGAACTTGCAGGTTTGTTAGGGCCACAGGATGTGGAGATCTGCa                                                                                                                                                <  1:188545/149‑1 (MQ=255)

   ttAGCCATTTCCACCCGTGGCAACAGCCGCGATATTGTTAAAGCAGTTGAAGCCGCCGTTACGCGTGATATGACCATTGTGGCATTGACCGGCTATGACGGCGGCGAACTTGCAGGTTTGTTAGGGCCACAGGATGTGGAGATCTGCAtt                                                                                                                                              <  1:393257/150‑1 (MQ=255)

        cATTTCCACCCGTGGCAACAGCCGCGATATTGTTAAAGCAGTTGAAGCCGCCGTTACGCGTGATATGACCATTGTGGCATTGACCGGCTATGACGGCGGCGAACTTGCAGGTTTGTTAGGGCCACAGGATGTGGAGATCTGCATTCCTTCg                                                                                                                                        >  1:179134/1‑151 (MQ=255)

            tCCACCCGTGGCAACAGCCGCGATATTGTTAAAGCAGTTGAAGCCGCCGTTACGCGTGATATGACCATTGTGGCATTGACCGGCTATGACGGCGGCGAACTTGCAGGTTTGTTAGGGCCACAGGATGTGGAGATCTGCATTCCTTCGCATc                                                                                                                                    >  2:128386/1‑151 (MQ=255)

             ccccccGTGGCAACAGCCGCGATATTGTTAAAGCAGTTGAAGCCGCCGTTACGCGTGATATGACCATTGTGGCATTGACCGGCTATGACGGCGGCGAACTTGCAGGTTTGTTAGGGCCACAGGATGTGGAGATCTGCATTCCTTCGCAt                                                                                                                                     <  1:626945/146‑1 (MQ=255)

              cACCCGTGGCAACAGCCGCGATATTGTTAAAGCAGTTGAAGCCGCCGTTACGCGTGATATGACCATTGTGGCATTGACCGGCTATGACGGCGGCGAACTTGCAGGTTTGTTAGGGCCACAGGATGTGGAGATCTGCATTCCTTCGCATCGt                                                                                                                                  >  2:299273/1‑151 (MQ=255)

                 ccGTGGCAACAGCCGCGATATTGTTAAAGCAGTTGAAGCCGCCGTTACGCGTGATATGACCATTGTGGCATTGACCGGCTATGACGGCGGCGAACTTGCAGGTTTGTTAGGGCCACAGGATGTGGAGATCTGCATTCCTTCGCATCGTAg                                                                                                                                >  2:629230/1‑150 (MQ=255)

                   gTGGCAACAGCCGCGATATTGTTAAAGCAGTTGAAGCCGCCGTTACGCGTGATATGACCATTGTGGCATTGACCGGCTATGACGGCGGCGAACTTGCAGGTTTGTTAGGGCCACAGGATGTGGAGATCTGCATTCCTTCGCATCGTAGTGc                                                                                                                             >  1:206147/1‑151 (MQ=255)

                   gTGGCAACAGCCGCGATATTGTTAAAGCAGTTGAAGCCGCCGTTACGCGTGATATGACCATTGTGGCATTGACCGGCTATGACGGCGGCGAACTTGCAGGTTTGTTAGGGCCACAGGATGTGGAGATCTGCATTCCTTCGCATCGTAGTGc                                                                                                                             >  1:376305/1‑151 (MQ=255)

                        aaCAGCCGCGATATTGTTAAAGCAGTTGAAGCCGCCGTTACGCGTGATATGACCATTGTGGCATTGACCGGCTATGACGGCGGCGAACTTGCAGGTTTGTTAGGGCCACAGGATGTGGAGATCTGCATTCCTTCGCATCGTAGTGCTCGc                                                                                                                         >  1:609297/1‑150 (MQ=255)

                                   tatTGTTAAAGCAGTTGAAGCCGCCGTTACGCGTGATATGACCATTGTGGCATTGACCGGCTATGACGGCGGCGAACTTGCAGGTTTGTTAGGGCCACAGGATGTGGAGATCTGCATTCCTTCGCATCGTAGTGCTCGCATTCAGGAAATg                                                                                                             >  2:201611/1‑151 (MQ=255)

                                     ttGTTAAAGCAGTTGAAGCCGCCGTTACGCGTGATATGACCATTGTGGCATTGACCGGCTATGACGGCGGCGAACTTGCAGGTTTGTTAGGGCCACAGGATGTGGAGATCTGCATTCCTTCGCATCGTAGTGCTCGCATTCAGGAAATGc                                                                                                            <  2:831323/150‑1 (MQ=255)

                                          aaaGCATTTGAAGCCGCCGTTACGCGTGATATGACCATTGTGGCATTGACCGGCTATGACGGCGGCGAACTTGCAGGTTTGTTAGGGCCACAGGATGTGGAGATCTGCATTCCTTCGCATCGTAGTGCTCGCATTCAGGAAATGCATATg                                                                                                       <  1:173678/150‑1 (MQ=255)

                                          aaaGCAGTTGAAGCCGCCGTTACGCGTGATATGACCATTGTGGCATTGACCGGCTATGACGGCGGCGAACTTGCAGGTTTGTTAGGGCCACAGGATGTGGAGATCTGCATTCCTTCGCATCGTAGTGCTCGCATTCAGGAAATGCATATg                                                                                                       <  1:127199/150‑1 (MQ=255)

                                           aaGCAGTTGAAGCCGCCGTTACGCGTGATATGACCATTGTGGCATTGACCGGCTATGACGGCGGCGAACTTGCAGGTTTGTTAGGGCCACAGGATGTGGAGATCTGCATTCCTTCGCATCGTAGTGCTCGCATTCAGGAAATGCATATGCt                                                                                                     <  1:348670/151‑1 (MQ=255)

                                           aaGCAGTTAAAGCCGCCGTTACGCGTGATATGACCATTGTGGCATTGACCGGCTATGACGGCGGCGAACTTGCAGGTTTGTTAGGGCCACAGGATGTGGAGATCTGCATTCCTTCGCATCGTAGTGCTCGCATTCAGGAAATGCat                                                                                                          <  1:450282/146‑1 (MQ=255)

                                                        cgcggTTACGCGTGATATGACCATTGTGGCATTGGCCGGCTATGACGGCGGCGAACTTGCAGGTTTGTTAGGGCCACAGGATGTGGAGATCTGCATTCCTTCGCATCGTAGTGCTCGCATTCAGGAAATGCATATGCTGACGGTAAATTGc                                                                                        <  2:341359/151‑1 (MQ=255)

                                                        cgccgTTACGCGTGATATGACCATTGTGGCATTGACCGGCTATGACGGCGGCGAACTTGCAGGTTTGTTAGGGCCACAGGATGTGGAGATCTGCATTCCTTCGCATCGTAGTGCTCGCATTCAGGAAATGCATATGCTGACGGTAAATTGc                                                                                        <  1:643953/151‑1 (MQ=255)

                                                         gccgTTACGCGTGATATGACCATTGTGGCATTGACCGGCTATGACGGCGGCGAACTTGCAGGTTTGTTAGGGCCACAGGATGTGGAGATCTGCATTCCTTCGCATCGTAGTGCTCGCATTCAGGAAATGCATATGCTGACGGTAAATTGcc                                                                                       >  2:366632/1‑151 (MQ=255)

                                                          ccgTTACGCGTGATATGACCATTGTGGCATTGACCGGCTATGACGGCGGCGAACTTGCAGGTTTGTTAGGGCCACAGGATGTGGAGATCTGCATTCCTTCGCATCGTAGTGCTCGCATTCAGGAAATGCATATGCTGACGGTAAATTGCCt                                                                                      >  2:687216/1‑151 (MQ=255)

                                                          ccgTTACGCGTGATATGACCATTGTGGCATTGACCGGCTATGACGGCGGCGAACTTGCAGGTTTGTTAGGGCCACAGGATGTGGAGATCTGCATTCCTTCGCATCGTAGTGCTCGCATTCAGGAAATGCATATGCTGACGGTAAATTGCCt                                                                                      <  2:191975/151‑1 (MQ=255)

                                                           cgTTACGCGTGATATGACCATTGTGGCATTGACCGGCTATGACGGCGGCGAACTTGCAGGTTTGTTAGGGCCACAGGATGTGGAGATCTGCATTCCTTCGCATCGTAGTGCTCGCATTCAGGAAATGCATATGCTGACGGTAAATTGCCtg                                                                                     >  1:781773/1‑151 (MQ=255)

                                                               aCGCGTGATATGACCATTGTGGCATTGACCGGCTATGACGGCGGCGAACTTGCAGGTTTGTTAGGGCCACAGGATGTGGAGATCTGCATTCCTTCGCATCGTAGTGCTCGCATTCAGGAAATGCATATGCTGACGGTAAATTGCCTGTGCg                                                                                 <  1:240305/151‑1 (MQ=255)

                                                                        atGACCATTGTGGCATTGACCGGCTATGACGGCGGCGAACTTGCAGGTTTGTTAGGGCCACAGGATGTGGAGATCTGCATTCCTTCGCATCGTAGTGCTCGCATTCAGGAAATGCATATGCTGACGGTAAATTGCCTGTGCGATCTGATc                                                                         <  2:236926/150‑1 (MQ=255)

                                                                        atGACCATTGTGGCATTGACCGGCTATGACGGCGGCGAACTTGCAGGTTTGTTAGGGCCACAGGATGTGGAGATCTGCATTCCTTCGCATCGTAGTGCTCGCATTCAGGAAATGCATATGCTGACGGTAAATTGCCTGTGCGATCTGATc                                                                         <  2:107396/150‑1 (MQ=255)

                                                                        atGACCATTGTGGCATTGACCGGCTATGACGGCGGCGAACTTGCAGGTTTGTTAGGGCCACAGGATGTGGAGATCTGCATTCCTTCGCATCGTAGTGCTCGCATTCAGGAAATGCATATGCTGACGGTAAATTGCCTGTGCGATCTGATCg                                                                        >  1:636319/1‑151 (MQ=255)

                                                                        atGACCAGTGTGGTATTGACCGGCGATGACGGCGGCGAACTTGCAGGTTTGTTAGGGCCACACGACGTGGTGATCTGCATTCCCTCGCATCGTAGTGCTCGCATTCACGAAATGCATATGCTGACGGTAAATTGCCTGTTCGATCTGaac                                                                         >  1:611458/1‑148 (MQ=255)

                                                                                 gtgGCATTGACCGGCTATGACGGCGGCGAACTTGCAGGTTTGTTAGGGCCACAGGATGTGGAGATCTGCATTCCTTCGCATCGTAGTGCTCGCATTCAGGAAATGCATATGCTGACGGTAAATTGCCTGTGCGATCTGATCGATAACACg                                                                <  1:85743/150‑1 (MQ=255)

                                                                                      aTTGACCGGCTATGACGGCGGCGAACTTGCAGGTTTGTTAGGGCCACAGGATGTGGAGATCTGCATTCCTTCGCATCGTAGTGCTCGCATTCAGGAAATGCATATGCTGACGGTAAATTGCCTGTGCGATCTGATCGATAACACGCTTTTc                                                          >  2:76741/1‑151 (MQ=255)

                                                                                            cGGCTATGACGGCGGCGAACTTGCAGGTTTGTTAGGGCCACAGGATGTGGAGATCTGCATTCCTTCGCATCGTAGTGCTCGCATTCAGGAAATGCATATGCTGACGGTAAATTGCCTGTGCGATCTGATCGATAACACGCTTTTCCCTCAc                                                    >  1:332823/1‑151 (MQ=255)

                                                                                                  tGACGGCGGCGAACTTGCAGGTTTGTTAGGGCCACAGGATGTGGAGATCTGCATTCCTTCGCATCGTAGTGCTCGCATTCAGGAAATGCATATGCTGACGGTAAATTGCCTGTGCGATCTGATCGATAACACGCTTTTCCCTCACCAGgat                                              >  1:142772/1‑151 (MQ=255)

                                                                                                    aCGGCGGCGAACTTGCAGGTTTGTTAGGGCCACAGGATGTGGAGATCTGCATTCCTTCGCATCGTAGTGCTCGCATTCAGGAAATGCATATGCTGACGGTAAATTGCCTGTGCGATCTGATCGATAACACGCTTTTCCCTCACCAGgatgt                                            <  1:848162/151‑2 (MQ=255)

                                                                                                      ggcggcGAACTTGCAGGTTTGTTAGGGCCACAGGATGTGGAGATCTGCATTCCTTCGCATCGTAGTGCTCGCATTCAGGAAATGCATATGCTGACGGTAAATTGCCTGTGCGATCTGATCGATAACACGCTTTTCCCTCACCAGGATGAtt                                          <  1:652710/151‑1 (MQ=255)

                                                                                                       gcggcgAACTTGCAGGTTTGTTAGGGCCACAGGATGTGGAGATCTGCATTCCTTCGCATCGTAGTGCTTGCATTCAGGAAATGCATATGCTGACGGTAAATTGCCTGTGCGATCTGATCGATAACTCGCTTTTCCCTCACCAGGAttatta                                         <  1:741412/151‑1 (MQ=255)

                                                                                                           cgAACTTGCAGGTTTGTTAGGGCCACAGGATGTGGAGATCTGCATTCCTTCGCATCGTAGTGCTCGCATTCAGGAAATGCATATGCTGACGGTAAATTGCCTGTGCGATCTGATCGATAACACGCTTTTCCCTCACCAGGATGATTAAGga                                     <  1:723362/151‑1 (MQ=255)

                                                                                                             aaCTTGCAGGTTTGTTAGGGCCACAGGATGTGGAGATCTGCATTCCTTCGCATCGTAGTGCTCGCATTCAGGAAATGCATATGCTGACGGTAAATTGCCTGTGCGATCTGATCGATAACACGCTTTTCCCTCACCAGGATGATTAAgg                                      >  1:631466/1‑148 (MQ=255)

                                                                                                             aaCTTGCAGGTTTGTTAGGGCCACAGGATGTGGAGATCTGCATTCCTTCGCATCGTAGTGCTCGCATTCAGGAAATGCATATGCTGACGGTAAATTGCCTGTGCGATCTGATCGATAACACGCTTTTCCCTCACCAGGATGATTAAgg                                      >  2:753736/1‑148 (MQ=255)

                                                                                                                  gCAGGTTTGTTAGGGCCGCAGGATGTTGAGATCTGCATTCCTTCGCATCGTAGTGCTCGCATTCAGGAAATGCATATGCTGACGGTAAATTGCCTGTGCGATCTGATCGATAACACGCTTTTCCCTCACCAGGATGATTAAGGAGAATAc                               <  1:86919/150‑1 (MQ=255)

                                                                                                                  gCAGGTTTGTTAGGGCCACAGGATGTGGAGATCTGCATTCCTTCGCATCGTAGTGCTCGCATTCAGGAAATGCATATGCTGACGGTAAATTGCCTGTGCGATCTGATCGATAACACGCTTTTCCCTCACCAGGATGATTAAGGAGAATACa                              <  2:631506/151‑1 (MQ=255)

                                                                                                                     ggTTTGTTAGGGCCACAGGATGTGGAGATCTGCATTCCTTCGCATCGTAGTGCTCGCATTCAGGAAATGCATATGCTGACGGTAAATTGCCTGTGCGATCTGATCGATAACACGCTTTTCCCTCACCAGGATGATTAAGGAGAATACATg                            >  1:830724/1‑150 (MQ=255)

                                                                                                                        ttGTTAGGGCCACAGGATGTGGAGATCTGCATTCCTTCGCATCGTAGTGCTCGCATTCAGGAAATGCATATGCTGACGGTAAATTGCCTGTGCGATCTGATCGATAACACGCTTTTCCCTCACCAGGATGATTAAGGAGAATACATGAAg                         <  1:657604/150‑1 (MQ=255)

                                                                                                                          gTTAGGGCCACAGGATGTGGAGATCTGCATTCCTTCGCATCGTAGTGCTCGCATTCAGGAAATGCATATGCTGACGGTAAATTGCCTGTGCGATCTGATCGATAACACGCTTTTCCCTCACCAGGATGATTAAGGAGAATACATGAAGGCa                      <  1:264274/151‑1 (MQ=255)

                                                                                                                                  cacaGGATGTGGAGATCTGCATTCCTTCGCATCGTAGTGCTCGCATTCAGGAAATGCATATGCTGACGGTAAATTGCCTGTGCGATCTGATCGATAACACGCTTTTCCCTCACCAGGATGATTAAGGAGAATACATGAAGGCATTATCGcc              >  2:339970/1‑151 (MQ=255)

                                                                                                                                      ggATGTGGAGATCTGCATTCCTTCGCATCGTAGTGCTCGCATTCAGGAAATGCATATGCTGACGGTAAATTGCCTGTGCGATCTGATCGATAACACGCTTTTCCCTCACCAGGATGATTAAGGAGAATACATGAAGGCATTATCGCCAATc          <  1:422236/151‑1 (MQ=255)

                                                                                                                                       gATGTGGAGATCTGCATTCCTTCGCATCGTAGTGCTCGCATTCAGGAAATGCATATGCTGACGGTAAATTGCCTGTGCGATCTGATCGATAACACGCTTTTCCCTCACCAGGATGATTAAGGAGAATACATGAAGGCATTATCGCCAATCg         <  2:581711/151‑1 (MQ=255)

                                                                                                                                        aTGTGGAGATCTGCATTCCTTCGCATCGTAGTGCTCGCATTCAGGAAATGCATATGCTGACGGTAAATTGCCTGTGCGATCTGATCGATAACACGCTTTTCCCTCACCAGGATGATTAAGGAGAATACATGAAGGCATTATCGCCAATc          >  1:339022/1‑149 (MQ=255)

                                                                                                                                        aTGTGGAGATCTGCATTCCTTCGCATCGTAGTGCTCGCATTCAGGAAATGCATATGCTGACGGTAAATTGCCTGTGCGATCTGATCGATAACACGCTTTTCCCTCACCAGGATGATTAAGGAGAATACATGAAGGCATTATCGCCAATCGc        <  1:596439/151‑1 (MQ=255)

                                                                                                                                            ggAGATCTGCATTCCTTCGCATCGTAGTGCTCGCATTCAGGAAATGCATATGCTGACGGTAAATTGCCTGTGCGATCTGATCGATAACACGCTTTTCCCTCACCAGGATGATTAAGGAGAATACATGAAGGCATTATCGCCAATCGCAGTc    <  2:206170/151‑1 (MQ=255)

                                                                                                                                            ggAGATCTGCATTCCTTCGCATCGTAGTGCTCGCATTCAGGAAATGCATATGCTGACGGTAAATTGCCTGTGCGATCTGATCGATAACACGCTTTTCCCTCACCAGGATGATTAAGGAGAATACATGAAGGCATTATCGCCAATCGCAGTc    <  2:390870/151‑1 (MQ=255)

                                                                                                                                              agaTCTGCATTCCTTCGCATCGTAGTGCTCGCATTCAGGAAATGCATATGCTGACGGTAAATTGCCTGTGCGATCTGATCGATAACACGCTTTTCCCTCACCAGGATGATTAAGGAGAATACATGAAGGCATTATCGCCAATCGCAGTCCt  <  1:164774/151‑1 (MQ=255)

TTGTTAGCCATTTCCACCCGTGGCAACAGCCGCGATATTGTTAAAGCAGTTGAAGCCGCCGTTACGCGTGATATGACCATTGTGGCATTGACCGGCTATGACGGCGGCGAACTTGCAGGTTTGTTAGGGCCACAGGATGTGGAGATCCGCATTCCTTCGCATCGTAGTGCTCGCATTCAGGAAATGCATATGCTGACGGTAAATTGCCTGTGCGATCTGATCGATAACACGCTTTTCCCTCACCAGGATGATTAAGGAGAATACATGAAGGCATTATCGCCAATCGCAGTCCT  >  NC_000913/3296145‑3296437

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A