Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A4 F50 I1 R1	14	47.9	1492993	99.0%	1478063	150.2

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,230,727	C→A	intergenic (‑333/‑40)	hlyE ← / → umuD	hemolysin E/translesion error‑prone DNA polymerase V subunit; RecA‑activated auto‑protease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,230,727	0	C	A	100.0%	187.3 / NA	48	intergenic (‑333/‑40)	hlyE/umuD	hemolysin E/translesion error‑prone DNA polymerase V subunit; RecA‑activated auto‑protease
Reads supporting (aligned to +/- strand): ref base C (0/0); new base A (15/33); total (15/33)

AGCCGGGTGATATTTTTCATTTCTGCTGGATGAGCGTCGTCGCCAGAAGGCCACGTGAGCACAAGATAAGAGAACGAAAAATCAGCAGCCTATGCAGCGACAAATATTGATAGCCTGAATCAGTATTGATCTGCTGGCAAGAACAGACTACTGTATATAAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGTGTGGCTTTCCTTCACCGGCAGCAGATTACGT  >  NC_000913/1230580‑1230867

aGCCGGGTGATATTTTTCATTTCTGCTGGATGAGCGTCGTCGCCAGAAGGCCACGTGAGCACAAGATAAGAGAACGAAAAATCAGCAGCCTATGCAGCGACAAATATTGATAGCCTGAATCAGTATTGATCTGCTGGCAAGAACAGaata                                                                                                                                            >  1:694395/1‑150 (MQ=255)

aGCCGGGTGATATTTTTCATTTCTGCTGGATGAGCGTCGTCGCCAGAAGGCCACGTGAGCACAAGATAAGAGAACGAAAAATCAGCAGCCTATGCAGCGACAAATATTGATAGCCTGAATCAGTATTGATCTGCTGGCAAGAACAGaata                                                                                                                                            <  1:568334/150‑1 (MQ=255)

   cGGGTGATATTTTTCATTTCTGCTGGATGAGCGTCGTCGCCAGAAGGCCACGTGAGCACAAGATAAGAGAACGAAAAATCAGCAGCCTATGCAGCGACAAATATTGATAGCCTGAATCAGTATTGATCTGCTGGCAAGAACAGAATACTGt                                                                                                                                        <  1:221659/151‑1 (MQ=255)

     ggTGATATTTTTCATTTCTGCTGGATGAGCGTCGTCGCCAGAAGGCCACGTGAGCACAAGATAAGAGAACGAAAAATCAGCAGCCTATGCAGCGACAAATATTGATAGCCTGAATCAGTATTGATCTGCTGGCAAGAACAGAATACTGtat                                                                                                                                      <  2:453620/151‑1 (MQ=255)

      gTGATATTTTTCATTTCTGCTGGATGAGCGTCGTCGCCAGAAGGCCACGTGAGCACAAGATAAGAGAACGAAAAATCAGCAGCCTATGCAGCGACAAATATTGATAGCCTGAATCAGTATTGATCTGCTGGCAAGAACAGAATACTGtat                                                                                                                                      >  1:271203/1‑150 (MQ=255)

             ttttCATTTCTGCTGGATGAGCGTCGTCGCCAGAAGGCCACGTGAGCACAAGATAAGAGAACGAAAAATCAGCAGCCTATGCAGCGACAAATATTGATAGCCTGAATCAGTATTGATCTGCTGGCAAGAACAGAATACTGTATATaaaaa                                                                                                                               <  1:353806/150‑1 (MQ=255)

                 cATTTCTGCTGGATGAGCGTCGTCGCCAGAAGGCCACGTGAGCACAAGATAAGAGAACGAAAAATCAGCAGCCTATGCAGCGACAAATATTGATAGCCTGAATCAGTATTGATCTGCTGGCAAGAACAGAATACTGTATATAAAAACAGt                                                                                                                           <  1:12548/150‑1 (MQ=255)

                    ttCTGCTGGATGAGCGTCGTCGCCAGAAGGCCACGTGAGCACAAGATAAGAGAACGAAAAATCAGCAGCCTATGCAGCGACAAATATTGATAGCCTGAATCAGTATTGATCTGCTGGCAAGAACAGAATACTGTATATAAAAACAGtata                                                                                                                        <  1:220977/150‑1 (MQ=255)

                        gctgGATGAGCGTCGTCGCCAGAAGGCCACGTGAGCACAAGATAAGAGAACGAAAAATCAGCAGCCTATGCAGCGACAAATATTGATAGCCTGAATCAGTATTGATCTGCTGGCAAGAACAGAATACTGTATATAAAAACAGTATAACtt                                                                                                                    <  1:599220/150‑1 (MQ=255)

                        gctgGATGAGCGTCGTCGCCAGAAGGCCACGTGAGCACAAGATAAGAGAACGAAAAATCAGCAGCCTATGCAGCGACAAATATTGATAGCCTGAATCAGTATTGATCTGCTGGCAAGAACAGAATACTGTATATAAAAACAGTATAACTTc                                                                                                                   >  2:107364/1‑151 (MQ=255)

                           ggATGAGCGTCGTCGCCAGAAGGCCACGTGAGCACAAGATAAGAGAACGAAAAATCAGCAGCCTATGCAGCGACAAATATTGATAGCCTGAATCAGTATTGATCTGCTGGCAAGAACAGAATACTGTATATAAAAACAGTATAACTTCAgg                                                                                                                >  1:352401/1‑151 (MQ=255)

                           ggATGAGCGTCGTCGCCAGAAGGCCACGTGAGCACAAGATAAGAGAACGAAAAATCAGCAGCCTATGCAGCGACAAATATTGATAGCCTGAATCAGTATTGATCTGCTGGCAAGAACAGAATACTGTATATAAAAACAGTATAACTTCAgg                                                                                                                <  1:382072/151‑1 (MQ=255)

                            gATGAGCGTCGTCGCCAGAAGGCCACGTGAGCACAAGATAAGAGAACGAAAAATCAGCAGCCTATGCAGCGACAAATATTGATAGCCTGAATCAGTATTGATCTGCTGGCAAGAACAGAATACTGTATATAAAAACAGTATAACTTCAGGc                                                                                                               >  1:566660/1‑151 (MQ=255)

                                   gtcgtcGCCAGAAGGCCACGTGAGCACAAGATAAGAGAACGAAAAATCAGCAGCCTATGCAGCGACAAATATTGATAGCCTGAATCAGTATTGATCTGCTGGCAAGAACAGAATACTGTATATAAAAACAGTATAACTTCAGGCAGattat                                                                                                        <  1:130782/151‑1 (MQ=255)

                                      gtcgCCAGAAGGCCACGTGAGCACAAGATAAGAGAACGAAAAATCAGCAGCCTATGCAGCGACAAATATTGATAGCCTGAATCAGTATTGATCTGCTGGCAAGAACAGAATACTGTATATAAAAACAGTATAACTTCAGGCAGATtattat                                                                                                     >  1:71325/1‑151 (MQ=255)

                                         gCCAGAAGGCCACGTGAGCACAAGATAAGAGAACGAAAAATCAGCAGCCTATGCAGCGACAAATATTGATAGCCTGAATCAGTATTGATCTGCTGGCAAGAACAGAATACTGTATATAAAAACAGTATAACTTCAGGCAGATTATTAtgtt                                                                                                  <  2:304460/151‑1 (MQ=255)

                                         gCCAGAAGGCCACGTGAGCACAAGATAAGAGAACGAAAAATCAGCAGCCTATGCAGCGACAAATATTGATAGCCTGAATCAGTATTGATCTGCTGGCAAGAACAGAATACTGTATATAAAAACAGTATAACTTCAGGCAGATTATTAtgtt                                                                                                  <  2:142344/151‑1 (MQ=255)

                                          ccAGAAGGCCACGTGAGCACAAGATAAGAGAACGAAAAATCAGCAGCCTATGCAGCGACAAATATTGATAGCCTGAATCAGTATTGATCTGCTGGCAAGAACAGAATACTGTATATAAAAACAGTATAACTTCAGGCAGATTATTAtgttg                                                                                                 <  1:502644/151‑1 (MQ=255)

                                             gAAGGCCACGTGAGCACAAGATAAGAGAACGAAAAATCAGCAGCCTATGCAGCGACAAATATTGATAGCCTGAATCAGTATTGATCTGCTGGCAAGAACAGAATACTGTATATAAAAACAGTATAACTTCAGGCAGATTATTATGTTGttt                                                                                              <  2:116182/151‑1 (MQ=255)

                                             gAAGGCCACGTGAGCACAAGATAAGAGAACGAAAAATCAGCAGCCTATGCAGCGACAAATATTGATAGCCTGAATCAGTATTGATCTGCTGGCAAGAACAGAATACTGTATATAAAAACAGTATAACTTCAGGCAGATTATTATGTTGttt                                                                                              <  1:726559/151‑1 (MQ=255)

                                              aaGGCCACGTGAGCACAAGATAAGAGAACGAAAAATCAGCAGCCTATGCAGCGACAAATATTGATAGCCTGAATCAGTATTGATCTGCTGGCAAGAACAGAATACTGTATATAAAAACAGTATAACTTCAGGCAGATTATTATGTTGttt                                                                                              >  2:2191/1‑150 (MQ=255)

                                               aGGCCACGTGAGCACAAGATAAGAGAACGAAAAATCAGCAGCCTATGCAGCGACAAATATTGATAGCCTGAATCAGTATTGATCTGCTGGCAAGAACAGAATACTGTATATAAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTAt                                                                                            <  2:446137/151‑1 (MQ=255)

                                                   cACGTGAGCACAAGATAAGAGAACGAAAAATCAGCAGCCTATGCAGCGACAAATATTGATAGCCTGAATCAGTATTGATCTGCTGGCAAGAACAGAATACTGTATATAAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAg                                                                                        <  1:311854/151‑1 (MQ=255)

                                                      gTGAGCACAAGATAAGAGAACGAAAAATCAGCAGCCTATGCAGCGACAAATATTGATAGCCTGAATCAGTATTGATCTGCTGGCAAGAACAGAATACTGTATATAAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCt                                                                                     >  1:480580/1‑151 (MQ=255)

                                                       tGAGCACAAGATAAGAGAACGAAAAATCAGCAGCCTATGCAGCGACAAATATTGATAGCCTGAATCAGTATTGATCTGCTGGCAAGAACAGAATACTGTATATAAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTg                                                                                    >  2:100218/1‑151 (MQ=255)

                                                        gAGCACAAGATAAGAGAACGAAAAATCAGCAGCCTATGCAGCGACAAATATTGATAGCCTGAATCAGTATTGATCTGCTGGCAAGAACAGAATACTGTATATAAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTg                                                                                    <  1:664042/150‑1 (MQ=255)

                                                        gAGCACAAGATAAGAGAACGAAAAATCAGCAGCCTATGCAGCGACAAATATTGATAGCCTGAATCAGTATTGATCTGCTGGCAAGAACAGAATACTGTATATAAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGc                                                                                   <  2:684235/151‑1 (MQ=255)

                                                           cacaAGATAAGAGAACGAAAAATCAGCAGCCTATGCAGCGACAAATATTGATAGCCTGAATCAGTATTGATCTGCTGGCAAGAACAGAATACTGTATATAAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCgg                                                                                 >  2:311930/1‑150 (MQ=255)

                                                                 aTAAGAGAACGAAAAATCAGCAGCCTATGCAGCGACAAATATTGATAGCCTGAATCAGTATTGATCTGCTGGCAAGAACAGAATACTGTATATAAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCcg                                                                          >  2:539798/1‑151 (MQ=255)

                                                                          cGAAAAATCAGCAGCCTATGCAGCGACAAATATTGATAGCCTGAATCAGTATTGATCTGCTGGCAAGAACAGAATACTGTATATAAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATtgt                                                                 <  2:582891/151‑1 (MQ=255)

                                                                          cGAAAAATCAGCAGCCTATGCAGCGACAAATATTGATAGCCTGAATCAGTATTGATCTGCTGGCAAGAACAGAATACTGTATATAAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATtgt                                                                 <  2:705663/151‑1 (MQ=255)

                                                                            aaaaaTCAGCAGCCTATGCAGCGACAAATATTGATAGCCTGAATCAGTATTGATCTGCTGGCAAGAACAGAATACTGTATATAAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGa                                                               <  2:386266/151‑1 (MQ=255)

                                                                                          tATGCAGCGACAAATATTGATAGCCTGAATCAGTATTGATCTGCTGGCAAGAACAGAATACTGTATATAAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTAtt                                                  <  1:582703/150‑1 (MQ=255)

                                                                                           aTGCAGCGACAAATATTGATAGCCTGAATCAGTATTGATCTGCTGGCAAGAACAGAATACTGTATATAAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTAtt                                                  <  1:110509/149‑1 (MQ=255)

                                                                                               aGCGACAAATATTGATAGCCTGAATCAGTATTGATCTGCTGGCAAGAACAGAATACTGTATATAAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGa                                            <  1:608982/151‑1 (MQ=255)

                                                                                                gCGACAAATATTGATAGCCTGAATCAGTATTGATCTGCTGGCAAGAACAGAATACTGTATATAAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGa                                            <  2:538134/150‑1 (MQ=255)

                                                                                                gCGACAAATATTGATAGCCTGAATCAGTATTGATCTGCTGGCAAGAACAGAATACTGTATATAAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGAt                                           <  1:231782/151‑1 (MQ=255)

                                                                                                 cGACAAATATTGATAGCCTGAATCAGTATTGATCTGCTGGCAAGAACAGAATACTGTATATAAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATCGTGACTTTTCCGCTATTTAGCGATc                                          <  2:142231/151‑1 (MQ=255)

                                                                                                      aaTATTGATAGCCTGAATCAGTATTGATCTGCTGGCAAGAACAGAATACTGTATATAAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGtt                                     <  2:329326/151‑1 (MQ=255)

                                                                                                         atTGATAGCCTGAATCAGTATTGATCTGCTGGCAAGAACAGAATACTGTATATAAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCa                                   <  2:725133/150‑1 (MQ=255)

                                                                                                             aTAGCCTGAATCAGTATTGATCTGCTGGCAAGAACAGAATACTGTATATAAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGtgtg                              >  2:380206/1‑151 (MQ=255)

                                                                                                               aGCCTGAATCAGTATTGATCTGCTGGCAAGAACAGAATACTGTATATAAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGTGTGGc                            >  1:145078/1‑151 (MQ=255)

                                                                                                                     aaTCAGTATTGATCTGCTGGCAAGAACAGAATACTGTATATAAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGTGTGGCTTTCCt                      <  2:284539/151‑1 (MQ=255)

                                                                                                                            aTTGATCTGCTGGCAAGAACAGAATACTGTATATAAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGTGTGGCTTTCCTTCACCg                <  1:383000/150‑1 (MQ=255)

                                                                                                                              tGATCTGCTGGCAAGAACAGAATACTGTATATAAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGTGTGGCTTTCCTTCACCGgc              >  2:194912/1‑150 (MQ=255)

                                                                                                                               gATCTGCTGGCAAGAACAGAATACTGTATATAAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGTGTGGCTTTCCTTCACCGgc              >  2:655959/1‑149 (MQ=255)

                                                                                                                                aTCTGCTGGCAAGAACAGAATACTGTATATAAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGTGTGGCTTTCCTTCACCGgca             <  1:398018/149‑1 (MQ=255)

                                                                                                                                    gctgGCAAGAACAGAATACTGTATATAAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGTGTGGCTTTCCTTCACCGGCAGCAGAt       <  2:592662/151‑1 (MQ=255)

                                                                                                                                       ggCAAGAACAGAATACTGTATATAAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGTGTGGCTTTCCTTCACCGGCAGCAGATTa     <  2:165926/150‑1 (MQ=255)

                                                                                                                                         cAAGAACAGAATACTGTATATAAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGTGTGGCTTTCCTTCACCGGCAGCAGATTACGt  >  1:691259/1‑151 (MQ=255)

AGCCGGGTGATATTTTTCATTTCTGCTGGATGAGCGTCGTCGCCAGAAGGCCACGTGAGCACAAGATAAGAGAACGAAAAATCAGCAGCCTATGCAGCGACAAATATTGATAGCCTGAATCAGTATTGATCTGCTGGCAAGAACAGACTACTGTATATAAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGTGTGGCTTTCCTTCACCGGCAGCAGATTACGT  >  NC_000913/1230580‑1230867

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A