Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A8 F50 I1 R1	163	55.0	1534755	98.6%	1513268	150.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,715,479	G→A	T19T (ACG→ACA)	eamB →	cysteine and O‑acetylserine exporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,715,479	0	G	A	100.0%	109.1 / NA	30	T19T (ACG→ACA)	eamB	cysteine and O‑acetylserine exporter
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (24/6); total (24/6)

AAACATTCATTTTTTTAAATGTTCCGTGTCGGGTACTGTCTACCAAAACAGAGGAGATAACAAGTGACACCGACCCTTTTAAGTGCTTTTTGGACTTACACCCTGATTACCGCTATGACGCCAGGACCGAACAATATTCTCGCCCTTAGCTCTGCTACGTCGCATGGATTTCGTCAAAGTACCCGCGTGCTGGCAGGGATGAGTCTGGGATTTTTGATTGTGATGTTACTGTGTGCGGGCATTTCATTTTCACTGGCAGTG  >  NC_000913/2715360‑2715620

gaaCATTCATTTTTTTAAATGGTCCGTGGCGGGGACTGTCTACCAAAACCGAGGAGATAACAAGTGACACCGACCCTTTTAAGAGCTTTTTGGACTTACACCCTGATTACCGCTATGACACCAGGAACGAACAATATTCTCGACCTTAGct                                                                                                                >  2:590347/2‑151 (MQ=255)

  aCATTCATTTTTTTAAATGTTCCGTGTCGGGTACTGTCTACCAAAACAGAGGAGATAACAAGTGACACCGACCCTTTTAAGTGCTTTTTGGACTTACACCCTGATTACCGCTATGACACCAGGACCGAACAATATTCTCGCCCTTAGctct                                                                                                              >  1:259091/1‑151 (MQ=255)

   cattcattTTTTTAAATGTTCCGTGTCGGGTACTGTCTACCAAAACAGAGGAGATAACAAGTGACACCGACCCTTTTAAGTGCTTTTTGGACTTACACCCTGATTACCGCTATGACACCAGGACCGAACAATATTCTCGCCCTTAGCTCTg                                                                                                             >  1:275817/1‑151 (MQ=255)

    attcattTTTTTAAATGTTCCGTGTCGGGTACTGTCTACCAAAACAGAGGAGATAACAAGTGACACCGACCCTTTTAAGTGCTTTTTGGACTTACACCCTGATTACCGCTATGACACCAGGACCGAACAATATTCTCGCCCTTAGCTCTGc                                                                                                            >  1:165527/1‑151 (MQ=255)

        attTTTTTAAATGTTCCGTGTCGGGTACTGTCTACCAAAACAGAGGAGATAACAAGTGACACCGACCCTTTTAAGTGCTTTTTGGACTTACACCCTGATTACCGCTATGACACCAGGACCGAACAATATTCTCGCCCTTAGCTCTGCTACg                                                                                                        >  2:581956/1‑151 (MQ=255)

           tttttAAATGTTCCGTGTCGGGTACTGTCTACCAAAACAGAGGAGATAACAAGTGACACCGACCCTTTTAAGTGCTTTTTGGACTTACACCCTGATTACCGCTATGACACCAGGACCGAACAATATTCTCGCCCTTAGCTCTGCTACGTCg                                                                                                     >  2:583130/1‑151 (MQ=255)

            ttttAAATGTTCCGTGTCGGGTACTGTCTACCAAAACAGAGGAGATAACAAGTGACACCGACCCTTTTAAGTGCTTTTTGGACTTACACCCTGATTACCGCTATGACACCAGGACCGAACAATATTCTCGCCCTTAGCTCTGCTACGTCGc                                                                                                    >  1:250654/1‑151 (MQ=255)

               tAAATGTTCCGTGTCGGGTACTGTCTACCAAAACAGAGGAGATAACAAGTGACACCGACCCTTTTAAGTGCTTTTTGGACTTACACCCTGATTACCGCTATGACACCAGGACCGAACAATATTCTCGCCCTTAGCTCTGCTACGTCGCATg                                                                                                 >  2:393355/1‑151 (MQ=255)

                  aTGTTCCGTGTCGGGTACTGTCTACCAAAACAGAGGAGATAACAAGTGACACCGACCCTTTTAAGTGCTTTTTGGACTTACACCCTGATTACCGCTATGACACCAGGACCGAACAATATTCTCGCCCTTAGCTCTGCTACGTCGCATGGAt                                                                                              >  1:687549/1‑151 (MQ=255)

                   tGTTCCGTGTCGGGTACTGTCTACCAAAACAGAGGAGATAACAAGTGACACCGACCCTTTTAAGTGCTTTTTGGACTTACACCCTGATTACCGCTATGACACCAGGACCGAACAATATTCTCGCCCTTAGCTCTGCTACGTCGCATGGAtt                                                                                             >  1:189420/1‑151 (MQ=255)

                     ttCCGTGTCGGGTACTGTCTACCAAAACAGAGGAGATAACAAGTGACACCGACCCTTTTAAGTGCTTTTTGGACTTACACCCTGATTACCGCTATGACACCAGGACCGAACAATATTCTCGCCCTTAGCTCTGCTACGTCGCATGGATTTc                                                                                           >  1:636321/1‑151 (MQ=255)

                     ttCCGTGTCGGGGACTGTCTACCAAAACAGAGGAGATAACAAGTGACACCGACCCTTTTAAGTGCTTTTTGGACTTACACCCTGATTACCGCTATGACACCAGGACCGAACAATATTCTCGCCCTTAGCTCTGCTACGTCGCATGGATTTc                                                                                           >  2:350497/1‑151 (MQ=255)

                      tCCGTGTCGGGTACTGTCTACCAAAACAGAGGAGATAACAAGTGACACCGACCCTTTTAAGTGCTTTTTGGACTTACACCCTGATTACCGCTATGACACCAGGACCGAACAATATTCTCGCCCTTAGCTCTGCTACGTCGCATGGATTTc                                                                                           <  1:278256/150‑1 (MQ=255)

                             cGGGTACTGTCTACCAAAACAGAGGAGATAACAAGTGACACCGACCCTTTTAAGTGCTTTTTGGACTTACACCCTGATTACCGCTATGACACCAGGACCGAACAATATTCTCGCCCTTAGCTCTGCTACGTCGCATGGATTTCGTCAAAg                                                                                    <  2:248489/150‑1 (MQ=255)

                               ggTACTGTCTACCAAAACAGAGGAGATAACAAGTGACACCGACCCTTTTAAGTGCTTTTTGGACTTACACCCTGATTACCGCTATGACACCAGGACCGAACAATATTCTCGCCCTTAGCTCTGCTACGTCGCATGGATTTCGTCAAAGTAc                                                                                 >  1:204763/1‑151 (MQ=255)

                                                   aggagATAACAAGTGACACCGACCCTTTTAAGTGCTTTTTGGACTTACACCCTGATTACCGCTATGACACCAGGACCGAACAATATTCTCGCCCTTAGCTCTGCTACGTCGCATGGATTTCGTCAAAGTACCCGCGTGCTGGCAGGGATg                                                              >  1:582346/1‑150 (MQ=255)

                                                          aaCAAGTGACACCGACCCTTTTAAGTGCTTTTTGGACTTACACCCTGATTACCGCTATGACACCAGGACCGAACAATATTCTCGCCCTTAGCTCTGCTACGTCGCATGGATTTCGTCAAAGTACCCGCGTGCTGGCAGGGATGAGTCTgg                                                       <  1:501130/150‑1 (MQ=255)

                                                            cAAGTGACACCGACCCTTTTAAGTGCTTTTTGGACTTACACCCTGATTACCGCTATGACACCAGGACCGAACAATATTCTCGCCCTTAGCTCTGCTACGTCGCATGGATTTCGTCAAAGTACCCGCGTGCTGGCAGGGATGAGTCTGGGAt                                                    >  1:584876/1‑151 (MQ=255)

                                                                   cacCGACCCTTTTAAGTGCTTTTTGGACTTACACCCTGATTACCGCTATGACACCAGGACCGAACAATATTCTCGCCCTTAGCTCTGCTACGTCGCATGGATTTCGTCAAAGTACCCGCGTGCTGGCAGGGATGAGTCTGGGATTTTTGAt                                             >  2:758410/1‑151 (MQ=255)

                                                                    acCGACCCTTTTAAGTGCTTTTTGGACTTACACCCTGATTACCGCTATGACACCAGGACCGAACAATATTCTCGCCCTTAGCTCTGCTACGTCGCATGGATTTCGTCAAAGTACCCGCGTGCTGGCAGGGATGAGTCTGGGATTTTTGAtt                                            <  2:582310/151‑1 (MQ=255)

                                                                      cGACCCTTTTAAGTGCTTTTTGGACTTACACCCTGATTACCGCTATGACACCAGGACCGAACAATATTCTCGCCCTTAGCTCTGCTACGTCGCATGGATTTCGTCAAAGTACCCGCGTGCTGGCAGGGATGAGTCTGGGATTTTTGATtgt                                          >  1:650728/1‑151 (MQ=255)

                                                                            ttttATGTGCTGTTTGGACTTACACCCTGATTTCCGCTATGACACCAGGACCGGACAATATTCTCGCCCTTAGCTCTGCTACGTCGCATGGATTTCGTCACAGTACCCGCGTGCTGGCAGTGATGAGTCTTGGAATTTTGATTGAGCTGtt                                    >  2:124681/1‑151 (MQ=255)

                                                                            ttttAAGTGCTTTTTGGACTTACACCCTGATTACCGCTATGACACCAGGACCGAACAATATTCTCGCCCTTAGCTCTGCTACGTCGCATGGATTTCGTCAAAGTGCCCGCGTGCTGGCAGGGATGAGTCTGGGATTTTTGATTGTGATGtt                                    >  2:630829/1‑151 (MQ=255)

                                                                             tttAAGTGCTTTTTGGACTTACACCCTGATTACCGCTATGACACCAGGACCGAACAATATTCTCGCCCTTAGCTCTGCTACGTCGCATGGATTTCGTCAAAGTACCCGCGTGCTGGCAGGGATGAGTCTGGGATTTTTGATTGTGATGtt                                    >  2:382733/1‑150 (MQ=255)

                                                                                aaGTGCTTTTTGGACTTACACCCTGATTACCGCTATGACACCAGGACCGAACAATATTCTCGCCCTTAGCTCTGCTACGTCGCATGGATTTCGTCAAAGTACCCGCGTGCTGGCAGGGATGAGTCTGGGATTTTTGATTGTGATGTTACt                                 <  2:259080/150‑1 (MQ=255)

                                                                                          tGGACTTACACCCTGATTACCGCTATGACACCAGGACCGAACAATATTCTCGCCCTTAGCTCTGCTACGTCGCATGGATTTCGTCAAAGTACCCGCGTGCTGGCAGGGATGAGTCTGGGATTTTTGATTGTGATGTTACTGTGTGCGGGc                       >  2:685943/1‑150 (MQ=255)

                                                                                            gACTTACACCCTGATTACCGCTATGACACCAGGACCGAACAATATTCTCGCCCTTAGCTCTGCTACGTCGCATGGATTTCGTCAAAGTACCCGCGTGCTGGCAGGGATGAGTCTGGGATTTTTGATTGTGATGTTACTGTGTGCGGGcatt                    >  1:272957/1‑151 (MQ=255)

                                                                                                             ccGCTATGACACCAGGACCGAACAATATTCTCGCCCTTAGCTCTGCTACGTCGCATGGATTTCGTCAAAGTACCCGCGTGCTGGCAGGGATGAGTCTGGGATTTTTGATTGTGATGTTACTGTGTGCGGGCATTTCATTTTCACTGGCAGt   >  1:596421/1‑151 (MQ=255)

                                                                                                              cGCTATGACACCAGGACCGAACAATATTCTCGCCCTTAGCTCTGCTACGTCGCATGGATTTCGTCAAAGTACCCGCGTGCTGGCAGGGATGAGTCTGGGATTTTTGATTGTGATGTTACTGTGTGCGGGCATTTCATTTTCACTGGCAGTg  <  1:685985/151‑1 (MQ=255)

                                                                                                               gCTATGACACCAGGACCGAACAATATTCTCGCCCTTAGCTCTGCTACGTCGCATGGATTTCGTCAAAGTACCCGCGTGCTGGCAGGGATGAGTCTGGGATTTTTGATTGTGATGTTACTGTGTGCGGGCATTTCATTTTCACTGGCAGTg  >  1:409384/1‑150 (MQ=255)

AAACATTCATTTTTTTAAATGTTCCGTGTCGGGTACTGTCTACCAAAACAGAGGAGATAACAAGTGACACCGACCCTTTTAAGTGCTTTTTGGACTTACACCCTGATTACCGCTATGACGCCAGGACCGAACAATATTCTCGCCCTTAGCTCTGCTACGTCGCATGGATTTCGTCAAAGTACCCGCGTGCTGGCAGGGATGAGTCTGGGATTTTTGATTGTGATGTTACTGTGTGCGGGCATTTCATTTTCACTGGCAGTG  >  NC_000913/2715360‑2715620

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A