Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A8 F50 I1 R1 163 55.0 1534755 98.6% 1513268 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,809,774 (T)7→6 coding (158/738 nt) ygaZ → putative L‑valine exporter, norvaline resistance protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,809,7680T.100.0% 122.6 / NA 26coding (152/738 nt)ygaZputative L‑valine exporter, norvaline resistance protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base . (23/3);  total (23/3)

CCACAGCCTGCTCCTGGTTCGGCGACCTTCATGGAAGGATGCAAAGACAGTTTACCGATTGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGTTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGAGTAGTTTGTGGATTGCTGCACTGACCGTCATGGCAATGGATGTTCGCCATGTGTTGTATGGCCCGTCACT  >  NC_000913/2809632‑2809906
                                                                                                                                        |                                                                                                                                          
ccACAGCCTGCTCCTGGTTCGGCGACCTTCATGGAAGGATGCAAAGACAGTTTACCGATTGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCG‑TTTTTTCTCCTGc                                                                                                                               <  1:600225/149‑1 (MQ=255)
 cacaGCCTGCTCCTGGTTCGGCGACCTTCATGGAAGGATGCAAAGACAGTTTACCGATTGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCG‑TTTTTTCTCCTGcatc                                                                                                                            >  2:178810/1‑151 (MQ=255)
  acaGCCTGCTCCTGGTTCGGCGACCTTCATGGAAGGATGCAAAGACAGTTTACCGATTGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCG‑TTTTTTCTCCTGcatc                                                                                                                            >  2:521523/1‑150 (MQ=255)
     gCCTGCTCCTGGTTCGGCGACCTTCATGGAAGGATGCAAAGACAGCTTACCGATTGTTATTAGTTATATTCAGGTGGCCTTTGCGTTAGGCCTGAATGCGACCCGTCTGGGATTCTCTCCTATCGAAAGCG‑TTTTTTCTCCTGCATCAttt                                                                                                                        >  2:428660/1‑151 (MQ=255)
                     gCGACCTTCATGGAAGGATGCAAAGACAGTTTACCGATTGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCG‑TTTTTTCTCCTGCATCATTTATGCAGGCGCGAGc                                                                                                          >  2:564184/1‑149 (MQ=255)
                      cGACCCTCATGGAAGGATGCAAAGACAGTTTACCGATTGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCG‑TTTTTTCTCCTGCATCATTTATGCAGGCGCGAGcc                                                                                                         >  2:111774/1‑149 (MQ=255)
                          cTTCATGGAAGGATGCAAAGACAGTTTACCGATTGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCG‑TTTTTTCTCCTGAATCATTTATGCAGGCGCGAGCCAGTTCg                                                                                                   >  2:537811/1‑151 (MQ=255)
                             cATGGCAGGATGCAAAGACAGTTTACCGATTGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCG‑TTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTc                                                                                                 >  2:197727/1‑150 (MQ=255)
                             cATGGAAGGATGCAAAGACAGTTTACCGATTGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCG‑TTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTc                                                                                                 >  2:28408/1‑150 (MQ=255)
                                      aTGCAAAGACAGTTTACCGATTGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCG‑TTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACcgcg                                                                                        >  1:129506/1‑150 (MQ=255)
                                      aTGCAAAGACAGTTTACCGATTGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCG‑TTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGa                                                                                       <  1:688014/151‑1 (MQ=255)
                                          aaaGACAGTTTACCGATTGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCG‑TTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCt                                                                                   >  2:287474/1‑151 (MQ=255)
                                            aGACAGTTTACCGATTGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCG‑TTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTgg                                                                                 >  2:217682/1‑151 (MQ=255)
                                                     aCCGATTGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCG‑TTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCggg                                                                         >  2:148860/1‑150 (MQ=255)
                                                            gTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCG‑TTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGAGTAGttt                                                                 >  1:32002/1‑151 (MQ=255)
                                                                 taGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCG‑TTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGAGTAGTTTGTgg                                                             >  1:620740/1‑150 (MQ=255)
                                                                 taGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCG‑TTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGAGTAGTTTGTgg                                                             >  2:11128/1‑150 (MQ=255)
                                                                  aGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCG‑TTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGAGTAGTTTGTGGAt                                                           >  2:167189/1‑151 (MQ=255)
                                                                     tataTTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCG‑TTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGAGTAGTTTGTGGATtgc                                                        >  2:213751/1‑151 (MQ=255)
                                                                                         gTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCG‑TTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGAGTAGTTTGTGGATTGCTGCACTGACCGTCATGGCa                                     >  1:673189/1‑150 (MQ=255)
                                                                                         gTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCG‑TTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGAGTAGTTTGTGGATTGCTGCACTGACCGTCATGGCa                                     >  2:371518/1‑150 (MQ=255)
                                                                                                  gAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCG‑TTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGAGTAGTTTGTGGATTGCTGCACTGACCGTCATGGCAATGGATGTTc                           >  1:348191/1‑151 (MQ=255)
                                                                                                        gACCCGTCTGGGATTCTCTCCTCTCGAAAGCG‑TTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGAGTAGTTTGTGGATTGCTGCACTGACCGTCATGGCAATGGATGTTCGCCAt                      >  2:290657/1‑150 (MQ=255)
                                                                                                         aCCCGTCTGGGATTCTCTCCTCTCGAAAGCG‑TTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGAGTAGTTTGTGGATTGCTGCACTGACCGTCATGGCAATGGATGTTCGCCAtgt                    >  2:311342/1‑151 (MQ=255)
                                                                                                            cGTCTGGGATTCTCTCCTCTCGAAAGCG‑TTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGAGTAGTTTGTGGATTGCTGCACTGACCGTCATGGCAATGGATGTTCGCCATGtgtt                 <  1:111776/151‑1 (MQ=255)
                                                                                                                           cctctcGAAAGCG‑TTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGAGTAGTTTGTGGATTGCTGCACTGACCGTCATGGCAATGGATGTTCGCCATGTGTTGTATGGCCCGTCACt  >  2:261517/1‑151 (MQ=255)
                                                                                                                                        |                                                                                                                                          
CCACAGCCTGCTCCTGGTTCGGCGACCTTCATGGAAGGATGCAAAGACAGTTTACCGATTGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGTTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGAGTAGTTTGTGGATTGCTGCACTGACCGTCATGGCAATGGATGTTCGCCATGTGTTGTATGGCCCGTCACT  >  NC_000913/2809632‑2809906

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A